Cryopreservation tube for exosome preparation

Abstract

The utility model relates to the technical field of exosome preparation cryopreservation, in particular to an exosome preparation cryopreservation tube which comprises a tube body, storage cavities are formed in the two ends of the tube body in a penetrating mode, scale marks are marked on the outer tube wall of the tube body, a separation assembly comprises separation pieces installed in the storage cavities in a lifting and sliding mode, and threaded shafts are connected to the separation pieces in a threaded penetrating and inserting mode. The two ends of the threaded shaft are both provided with rotating heads, the two sealing plugs are plugged and connected to the two ends of the storage cavity respectively, a partition piece capable of sliding in a lifting mode is arranged in the pipe body, the storage cavity of the pipe body is divided into an upper part and a lower part through the partition piece, and the size of the upper storage space and the size of the lower storage space are adjusted by adjusting the height of the partition piece. The use efficiency of the cryopreservation tube storage space is improved, and through the design of related limiting structures such as a rotating head and a butt joint groove, the partition pieces with the positions adjusted cannot move relative to the tube body, so that the two cryopreservation tube storage spaces are kept fixed after adjustment.

Description

Technical Field

[0001] This utility model relates to the field of exosome preparation cryopreservation technology, specifically to a cryopreservation tube for exosome preparations. Background Technology

[0002] The cryopreservation of exosome preparations is a key step in ensuring their stability and bioactivity. Selecting appropriate cryopreservation tubes and following standardized operating procedures are of utmost importance.

[0003] The cryopreservation procedure for exosome preparations involves mixing the exosome suspension with a cryoprotectant and then dispensing it into cryopreservation tubes (100-500 μL per tube).

[0004] Labeling: Clearly label the sample name, date, concentration, and other information (using a low-temperature resistant label or an oil-based pen);

[0005] Gradient cooling:

[0006] Programmed cooling: 4℃ (30min) → -20℃ (2h) → -80℃ (overnight) → long-term storage in liquid nitrogen;

[0007] Unprogrammed cooling system: Use an isopropanol cryoprotectant to slowly cool to -80°C;

[0008] Long-term storage: Transfer to **liquid nitrogen gas phase layer (below -150℃)** or -80℃ (short-term storage within 1 year);

[0009] Thawing Precautions:

[0010] Gently shake in a 37°C water bath until completely thawed (avoid repeated freeze-thaw cycles). If using DMSO, wash with PBS after thawing (by ultracentrifugation or filtration).

[0011] To optimize the proportions of exosome preparations and avoid repeated freeze-thaw cycles, the current practice is to repackage them into smaller portions. For example, the Chinese utility model patent with authorization announcement number "CN222090632U" specifically describes "a cryopreservation tube for exosome preparations". Through the separator, the entire tube can be stored in large quantities and used in multiple batches, which is less likely to cause the unused raw materials to be contaminated by the external environment.

[0012] However, the consistent spacing between the dividing components makes each storage chamber of the tube body completely identical in size, which makes it inconvenient to adjust the space of the corresponding storage chamber according to different usage requirements, thus reducing the storage space utilization efficiency of the cryopreservation tube. Utility Model Content

[0013] In view of the above-mentioned shortcomings of the existing technology, the present invention provides a cryopreservation tube for exosome preparations, which can effectively solve the problems mentioned in the background art.

[0014] To achieve the above objectives, this utility model provides the following technical solution:

[0015] This invention provides a cryopreservation tube for exosome preparations, including...

[0016] The tube has storage cavities extending through both ends, and scale lines are marked on the outer wall of the tube.

[0017] The partition assembly includes a partition plate that is lifted and slidably installed in the storage cavity. A threaded shaft is threaded through the partition plate, and a rotating head is installed at both ends of the threaded shaft.

[0018] Two sealing plugs are inserted into both ends of the storage cavity, and each of the two sealing plugs has a mating groove at one end facing each other to facilitate the fitting and snapping of the rotating head.

[0019] Furthermore, both ends of the tube are fixedly installed with mating rings, and each of the two sealing plugs has an annular cavity on one side facing each other. The two mating rings are respectively inserted into the corresponding annular cavities.

[0020] Furthermore, the partition assembly also includes fixed seats that are fixedly installed at the two openings of the storage cavity. The top of each fixed seat has an annular hole extending through the bottom, and the inner wall of each annular hole has an annular groove.

[0021] Furthermore, a rotating ring is rotatably installed inside the annular hole, and the outer ring walls of the two rotating rings are each fixedly fitted with annular rings that are rotatably installed in corresponding annular grooves.

[0022] Furthermore, a threaded shaft is fixedly connected between the two rotating rings, and the two rotating heads are fixedly connected to their respective rotating rings.

[0023] Furthermore, the outer ring wall of the separator is vertically fixedly connected with two centrally symmetrically distributed limiting heads, and the inner wall of the storage cavity is provided with two limiting grooves, with the two limiting heads slidingly installed in the corresponding limiting grooves respectively.

[0024] The technical solution provided by this utility model has the following advantages compared with the known prior art:

[0025] A movable partition is installed inside the tube to divide the storage cavity of the tube into upper and lower parts. By adjusting the height of the partition, the size of the upper and lower storage spaces can be adjusted, thereby improving the utilization efficiency of the cryopreservation tube storage space.

[0026] Furthermore, through the design of relevant limiting structures such as rotating heads and docking grooves, the spacers that have been adjusted will not move relative to the tube body, thereby keeping the storage space of the two cryopreservation tubes fixed after adjustment. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0028] Figure 1 This is a schematic diagram of the overall structure of the cryopreservation tube for the exosome preparation of this utility model;

[0029] Figure 2 This is a schematic diagram of the overall structure of the present invention after the sealing plug is opened;

[0030] Figure 3 This is a schematic diagram of the separator component structure of this utility model;

[0031] Figure 4 This is a schematic diagram of the mounting structure of the fixed base and rotating ring of this utility model;

[0032] Figure 5 This is a schematic diagram of the sealing plug structure of this utility model.

[0033] The labels in the diagram represent:

[0034] 1. Tube body; 11. Connecting ring; 12. Scale lines;

[0035] 21. Fixed base; 22. Rotating ring; 23. Threaded shaft; 24. Rotating head; 25. Separator;

[0036] 3. Sealing plug; 31. Annular cavity; 32. Connecting groove. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0038] The present invention will be further described below with reference to the embodiments.

[0039] Example 1

[0040] Reference Figure 1-5This is the first embodiment of the present invention, which discloses a cryopreservation tube for exosome preparations. The tube is characterized by comprising a tube body 1, with storage cavities extending through both ends of the tube body 1. The outer wall of the tube body 1 is marked with graduation lines 12, which must clearly indicate whether the graduation lines 12 refer to the volume of the exosome suspension (containing a preservative), rather than the volume of pure water (due to different coefficients of expansion at low temperatures). The separating assembly includes a separating plate 25 that is slidably mounted within the storage cavity. The sliding fit between the separating plate 25 and the inner wall of the storage cavity requires the addition of an elastic sealing ring (such as a silicone ring). A threaded shaft 23 is threaded through the separating plate 25, and rotating heads 24 are installed at both ends of the threaded shaft 23. Two sealing plugs 3 are respectively inserted into both ends of the storage cavity, and the sealing plugs 3 are made of low-temperature resistant silicone material.

[0041] Both sealing plugs 3 have mating grooves 32 at opposite ends to facilitate the fitting and engagement of the rotating head 24. Although the shape of the mating grooves 32 is adapted to the rotating head 24, the depth of the mating grooves 32 is greater than the length of the rotating head 24. This allows the mating grooves 32 to engage with the rotating head 24 without the sealing plugs 3 being fully inserted into the storage cavity opening. At the same time, when the sealing plugs 3 are inserted into the storage cavity opening, the rotating head 24 can still be inserted deep into the mating grooves 32.

[0042] Example 2

[0043] Reference Figure 1-5 This is the second embodiment of the present invention. The difference between this embodiment and the first embodiment is that: both ends of the tube body 1 are fixedly installed with docking rings 11, and the two sealing plugs 3 are provided with annular cavities 31 on opposite sides. The two docking rings 11 are respectively adapted to be inserted into the corresponding annular cavities 31. The insertion design of the docking rings 11 and the annular cavities 31 ensures that the seal can be maintained at low temperature to prevent liquid nitrogen from seeping in. The separation component also includes fixed seats 21 fixedly installed at the two openings of the storage cavity. The top of the two fixed seats 21 is provided with annular holes through the bottom, and the inner wall of the two annular holes is provided with annular grooves.

[0044] A rotating ring 22 is rotatably installed in the annular hole. The outer ring walls of the two rotating rings 22 are each fixedly fitted with annular rings that are rotatably installed in the corresponding annular grooves. A threaded shaft 23 is fixedly connected between the two rotating rings 22. Two rotating heads 24 are fixedly connected to the corresponding rotating rings 22 respectively.

[0045] Two centrally symmetrically distributed limiting heads are vertically fixed to the outer ring wall of the separator 25. Two limiting grooves are opened on the inner wall of the storage cavity, and the two limiting heads are slidably installed in the corresponding limiting grooves respectively.

[0046] The structures used in this application that come into contact with the exosome preparation, including but not limited to tube 1, are all made of polypropylene and can withstand liquid nitrogen at -150°C. The rotating head 24 is made of polycarbonate.

[0047] In a liquid nitrogen environment at -150℃, the threaded shaft 23 (made of polypropylene) may experience increased rotational resistance due to cold contraction or icing. Therefore, the threaded shaft 23 employs a large pitch design to reduce the risk of jamming.

[0048] The thread pitch of threaded shaft 23 is ≥2mm;

[0049] The remaining structure is the same as that in Example 1.

[0050] The working process of this utility model is as follows:

[0051] First, adjust the lifting height of the separator 25 according to the capacity of the exosome preparation to be stored. Specifically, place one of the sealing plugs 3 close to the end of the tube 1 so that the rotating head 24 is inserted into the corresponding docking groove 32. At this time, rotate the sealing plug 3, and drive the rotating ring 22 and the threaded shaft 23 to rotate through the snap-fit ​​design of the docking groove 32 and the rotating head 24. Since the separator 25 is threadedly fitted on the outer shaft wall of the threaded shaft 23, and the limiting head fixedly installed on the outer ring wall of the separator 25 is respectively adapted and slidably installed in the corresponding limiting groove, when the threaded shaft 23 rotates, the separator 25 is forced to slide up and down in the storage cavity to adjust the size of the two storage spaces after the storage cavity is divided to meet the capacity of the exosome preparation to be stored. During the adjustment process, the scale line 12 can help to know the size and volume of the two storage spaces after the separator 25 is adjusted.

[0052] Secondly, when the exosome preparation needs to be stored, the sealing plug 3, which has already been connected to the docking groove 32 and the rotating head 24, is pressed forcefully against the tube body 1, so that the docking ring 11 at the end of the tube body 1 is properly inserted into the annular cavity 31, thereby completing the sealing connection between the sealing plug 3 and the tube body 1. Then, the entire tube body 1 is flipped over so that the end of the storage cavity opening that has not yet been inserted with the sealing plug 3 is vertically upward. The exosome preparation is injected into the storage space, and the corresponding sealing plug 3 is inserted, ensuring that the docking groove 32 of the sealing plug 3 is properly inserted into the corresponding rotating head 24. The entire tube body 1 is flipped over again, the sealing plug 3 located at the top is removed, another part of the exosome preparation is injected into the storage space currently located at the top, and the sealing plug 3 is re-sealed and inserted into the storage cavity opening.

[0053] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of this utility model.

Claims

1. A cryopreservation tube for an exosome preparation, characterized in that, include The tube has storage cavities extending through both ends, and scale lines are marked on the outer wall of the tube. The partition assembly includes a partition plate that is slidably mounted in the storage cavity, and a threaded shaft is threaded through the partition plate, with a rotating head installed at both ends of the threaded shaft; Two sealing plugs are respectively inserted into both ends of the storage cavity, and each of the two sealing plugs has a mating groove at one end facing each other to facilitate the fitting and snapping of the rotating head.

2. The cryopreservation tube for the exosome preparation according to claim 1, characterized in that, Both ends of the tube are fixedly installed with docking rings, and the two sealing plugs each have an annular cavity on one side facing each other. The two docking rings are respectively inserted into the corresponding annular cavities in a matching manner.

3. The cryopreservation tube for the exosome preparation according to claim 1, characterized in that, The partition assembly also includes a fixing seat fixedly installed at the two openings of the storage cavity. The top of each fixing seat has an annular hole extending through the bottom, and the inner wall of each annular hole has an annular groove.

4. The cryopreservation tube for the exosome preparation according to claim 3, characterized in that, A rotating ring is rotatably installed inside the annular hole, and each of the two rotating rings has an annular ring fixedly fitted onto its outer ring wall, which is rotatably installed in a corresponding annular groove.

5. The cryopreservation tube for an exosome preparation according to claim 4, characterized in that, A threaded shaft is fixedly connected between the two rotating rings, and the two rotating heads are respectively fixedly connected to the corresponding rotating rings.

6. The cryopreservation tube for the exosome preparation according to claim 1, characterized in that, The outer ring wall of the separator is vertically fixedly connected to two centrally symmetrically distributed limiting heads, and the inner wall of the storage cavity is provided with two limiting grooves. The two limiting heads are respectively adapted to slide in the corresponding limiting grooves.

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