Plant explant liquid tissue culture device

Abstract

The utility model discloses a plant explant liquid tissue culture device which comprises a box body, an inner ring is fixedly connected to the inner wall of the box body, a large aseptic cotton piece is placed at the top of the inner ring, a suction cup and a limiting frame are fixedly connected to the inner bottom face of the box body, the suction cup is located in the limiting frame, and the large aseptic cotton piece is placed in the limiting frame. A culture assembly is arranged in the limiting frame, the culture assembly comprises a culture box, a placement ring, a sterile cotton piece and a small sterile cotton piece, the placement ring is fixedly connected to the inner wall of the culture box, and the sterile cotton piece is fixedly connected to the bottom of the placement ring. By arranging the box body, the inner ring, the large aseptic cotton piece, the culture boxes, the placing ring, the aseptic cotton piece and the small aseptic cotton piece, the large aseptic cotton piece is arranged in the box body, the small aseptic cotton piece is also arranged in each culture box, and air entering the culture boxes can be disinfected, so that external bacterial infection is reduced, and the survival rate of explants is increased.

Description

Technical Field

[0001] This utility model relates to the field of plant explant culture technology, and in particular to a liquid tissue culture device for plant explants. Background Technology

[0002] Explants refer to various inoculation materials used in plant tissue culture. Theoretically, plant cells are totipotent and capable of regenerating new plants; any organ, any tissue, single cell, and protoplast can serve as an explant. Liquid tissue culture of plant explants uses liquid culture media, which, unlike traditional solid media, do not contain solidifying agents such as agar. Explants grow in a liquid environment, resulting in faster growth.

[0003] Existing liquid tissue culture devices for plant explants lack protective measures during the explant culture process, and explants are often infected by external bacteria, resulting in low culture survival rates. Utility Model Content

[0004] The purpose of this invention is to provide a liquid tissue culture device for plant explants. Large sterile cotton pads are placed inside the box, and small sterile cotton pads are also placed inside each culture box. This sterilizes the air entering the culture box, thereby reducing external bacterial infection and improving the survival rate of explants. The culture boxes are placed inside a limiting frame, and suction cups are used to adhere to the bottom of the culture boxes, thus stabilizing them. The sterile cotton pads adhere to the inner wall of the culture boxes, allowing the explants to attach to the sterile cotton pads and grow, thus providing more growth space for the explants.

[0005] To achieve the above objectives, a liquid tissue culture device for plant explants is provided, comprising: a box body, an inner ring fixedly connected to the inner wall of the box body, a large sterile cotton pad placed on the top of the inner ring, a suction cup and a limiting frame fixedly connected to the inner bottom surface of the box body, the suction cup being located inside the limiting frame, a culture component disposed inside the limiting frame, the culture component including a culture box, a placement ring, a sterile cotton pad and a small sterile cotton pad, a placement ring fixedly connected to the inner wall of the culture box, a sterile cotton pad fixedly connected to the bottom of the placement ring, a small sterile cotton pad placed on the top of the placement ring, a top cover snapped onto the top of the box body, an air inlet pipe fixedly connected to the top of the top cover, and a filter screen fixedly connected inside the air inlet pipe.

[0006] According to the aforementioned liquid tissue culture device for plant explants, the culture box is located above the suction cup.

[0007] According to the aforementioned liquid tissue culture device for plant explants, the cross-section of the sterile cotton pad is "L" shaped.

[0008] According to the liquid tissue culture device for plant explants, there are seven limiting frames and seven suction cups, which are evenly distributed inside the box.

[0009] According to the aforementioned liquid tissue culture device for plant explants, the number of culture components is set to seven, and they are evenly distributed inside the box.

[0010] According to the aforementioned liquid tissue culture device for plant explants, the sterile cotton pad is attached to the inner wall of the culture box.

[0011] According to the aforementioned liquid tissue culture device for plant explants, the box, culture container, and top cover are all made of tempered glass.

[0012] According to the liquid tissue culture device for plant explants, the bottom of the box is fixedly connected with a support column, and the number of the support columns is three, which are evenly distributed at the bottom of the box.

[0013] The above-mentioned solution has the following beneficial effects:

[0014] 1. By setting up a box, inner ring, large sterile cotton pads, culture box, placement ring, sterile cotton pads and small sterile cotton pads, the large sterile cotton pads are placed inside the box, and the small sterile cotton pads are also placed inside each culture box, which can sterilize the air entering the culture box, thereby reducing external bacterial infection and improving the survival rate of explants.

[0015] 2. By setting suction cups and a limiting frame, the culture box is placed inside the limiting frame, and the suction cups are used to adhere to the bottom of the culture box, thereby stabilizing the culture box.

[0016] 3. By setting up placement rings and sterile cotton pads, the sterile cotton pads fit snugly against the inner wall of the culture box, allowing explants to attach to the sterile cotton pads and grow, thus providing more growth space for the explants.

[0017] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0018] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0019] Figure 1 This is a front view of a liquid tissue culture device for plant explants according to this utility model;

[0020] Figure 2 This is a schematic diagram of the cross-sectional structure of the box of a liquid tissue culture device for plant explants according to the present invention;

[0021] Figure 3This is a schematic cross-sectional view of the culture box structure of a liquid tissue culture device for plant explants according to this utility model;

[0022] Figure 4 This is a schematic diagram of the upper cover structure of a liquid tissue culture device for plant explants according to the present invention.

[0023] Legend:

[0024] 1. Box body; 2. Inner ring; 3. Large sterile cotton pad; 4. Suction cup; 5. Limiting frame; 6. Culture component; 7. Top cover; 8. Air inlet pipe; 9. Filter screen; 10. Support column; 601. Culture box; 602. Placement ring; 603. Sterile cotton pad; 604. Small sterile cotton pad. Detailed Implementation

[0025] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.

[0026] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0027] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0028] Reference Figure 1-4This utility model discloses a liquid tissue culture device for plant explants, comprising: a box 1, an inner ring 2 fixedly connected to the inner wall of the box 1, a large disinfectant cotton pad 3 placed on the top of the inner ring 2 for preliminary disinfection of the air entering the box 1, thereby reducing the probability of external infection; suction cups 4 and limiting frames 5 fixedly connected to the inner bottom surface of the box 1, the suction cups 4 being located inside the limiting frames 5; seven of each type of device, including the limiting frames 5 and suction cups 4, evenly distributed inside the box 1; and seven culture components 6 evenly distributed inside the limiting frames 5. Each culture component 6 includes a culture box 601 and a placement ring. 602, sterile cotton pads 603, and small disinfectant cotton pads 604 are included. The culture box 601 is located above the suction cup 4. A placement ring 602 is fixedly connected to the inner wall of the culture box 601. A sterile cotton pad 603 is fixedly connected to the bottom of the placement ring 602. The cross-section of the sterile cotton pad 603 is "L" shaped. The sterile cotton pad 603 fits against the inner wall of the culture box 601. The explant can attach to the sterile cotton pad 603 and grow, thus providing more growth space for the explant. A small disinfectant cotton pad 604 is placed on the top of the placement ring 602 to further disinfect the air entering the culture box 601 and reduce the probability of external infection.

[0029] The top of the housing 1 is snapped with a cover 7, and the top of the cover 7 is fixedly connected to an air inlet pipe 8. The inside of the air inlet pipe 8 is fixedly connected to a filter screen 9 to filter the incoming air and prevent dust and other impurities from entering.

[0030] The box 1, culture box 601 and top cover 7 are all made of tempered glass, which allows light to pass through and facilitates the growth of explants. The bottom of the box 1 is fixedly connected with three support columns 10, which are evenly distributed at the bottom of the box 1.

[0031] Working principle: All components are sterilized and then placed in a professional culture room for cultivation. The culture room provides suitable temperature, humidity, and light conditions. Liquid culture medium is added to the culture box 601, and explants are placed into each culture box 601. Then, small sterile cotton pads 604 are placed above the placement ring 602, and the culture box 601 is placed in the limiting frame 5. The bottom of the culture box 601 is then fixed by suction cups 4 to stabilize the culture box 601. Then, large sterile cotton pads 3 are placed on the inner ring 2, and the top cover 7 is closed. External air enters through the air inlet pipe 8, and the filter screen 9 filters the incoming air. After being sterilized by the large sterile cotton pads 3 and small sterile cotton pads 604, the air enters the culture box 601, reducing the probability of external infection. During the operation, the operator needs to disinfect the operating equipment to ensure compliance with laboratory operating procedures. The contents not described in detail in this manual are existing technologies known to those skilled in the art.

[0032] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A liquid tissue culture device for plant explants, comprising: The box body (1) is characterized in that an inner ring (2) is fixedly connected to the inner wall of the box body (1), a large sterile cotton pad (3) is placed on the top of the inner ring (2), a suction cup (4) and a limiting frame (5) are fixedly connected to the inner bottom surface of the box body (1), the suction cup (4) is located inside the limiting frame (5), and a culture component (6) is arranged inside the limiting frame (5). The culture component (6) includes a culture box (601), a placement ring (602), and a sterile cotton pad (603). 03) and small disinfectant cotton pads (604), a placement ring (602) is fixedly connected to the inner wall of the culture box (601), a sterile cotton pad (603) is fixedly connected to the bottom of the placement ring (602), a small disinfectant cotton pad (604) is placed on the top of the placement ring (602), a top cover (7) is snapped onto the top of the box body (1), an air inlet pipe (8) is fixedly connected to the top of the top cover (7), and a filter screen (9) is fixedly connected inside the air inlet pipe (8).

2. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The culture box (601) is located above the suture cup (4).

3. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The cross-section of the sterile cotton pad (603) is "L" shaped.

4. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The number of the limiting frame (5) and the suction cup (4) are both set to seven, and they are evenly distributed inside the box (1).

5. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The number of culture components (6) is set to seven, and they are evenly distributed inside the box (1).

6. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The sterile cotton pad (603) is attached to the inner wall of the culture box (601).

7. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The box (1), culture box (601) and top cover (7) are all made of tempered glass.

8. The liquid tissue culture device for plant explants according to claim 1, characterized in that, The bottom of the box (1) is fixedly connected to a support column (10), and the number of the support column (10) is three, which are evenly distributed at the bottom of the box (1).

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