Protein centrifugal extraction device

Abstract

The utility model discloses a protein centrifugal extraction device, which belongs to the field of protein extraction equipment and comprises a shell, a rotating shaft is rotatably mounted at the bottom in the shell, a supporting plate is fixedly mounted at the top of the rotating shaft, a flexible pad is fixedly connected to the top of the supporting plate, and two vertical rods distributed at intervals are fixedly mounted on the supporting plate. Limiting rings are fixedly connected to the vertical rods correspondingly, and a driving mechanism is installed on the shell. A first container placed on the flexible pad is arranged in the shell, the upper end of the first container extends outwards, the upper end of the vertical rod penetrates through the upper end of the first container, the limiting ring is located below the upper end of the first container, and an annular frame is fixedly installed at the lower end of the first container. According to the protein centrifugal extraction device, the first container is limited in the vertical direction, the first container can be prevented from shaking up and down, the first container is prevented from being broken, and equipment is prevented from being damaged, so that the experiment safety is improved, biological pollution diffusion is avoided, and centrifugal accidents are eradicated.

Description

Technical Field

[0001] This utility model relates to the field of protein extraction equipment technology, and in particular to a protein centrifugal extraction device. Background Technology

[0002] Protein extraction by high-speed centrifugation is a commonly used technique in laboratories. Its core lies in using the powerful centrifugal force generated by high-speed rotation to separate proteins from other components based on differences in density and sedimentation coefficient.

[0003] A high-speed centrifuge for protein extraction, with publication number CN119680776A, is disclosed, specifically including a centrifuge rotor, an anti-contamination adsorption pad, and a limiting storage seat. The centrifuge rotor is installed at the axial position inside the high-speed centrifuge. A limiting plate is installed on the top of the centrifuge rotor. Multiple rotation adjustment heads are evenly distributed on the outer wall of the limiting plate. A linkage pushing mechanism is installed at the bottom of the limiting plate. An adjustment cover is installed on the top of the limiting plate, and the adjustment cover is used to control the linkage pushing mechanism. The anti-contamination adsorption pad is installed between the limiting plate and the adjustment cover, and the adsorption ends of the anti-contamination adsorption pad are distributed on the rotation adjustment heads. The limiting storage seat is located on the side of the high-speed centrifuge.

[0004] Existing protein centrifugation extraction devices require centrifuge tubes containing crude protein extract to be placed inside the centrifuge during operation. The centrifuge tubes rotate at high speed during centrifugation, and may vibrate due to factors such as sample balancing errors or operational errors, causing the centrifuge tubes to collide with the inside of the centrifuge and thus damaging the centrifuge tubes and the centrifuge. To solve the above problems, we propose a protein centrifugation extraction device. Utility Model Content

[0005] The purpose of this invention is to solve the problem of centrifuge tubes colliding with the inside of the centrifuge during centrifugation due to shaking in the existing technology, and to propose a protein centrifugation extraction device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A protein centrifugation extraction device includes a housing, a rotating shaft rotatably mounted at the bottom of the housing, a support plate fixedly mounted at the top of the rotating shaft, a flexible pad fixedly connected to the top of the support plate, two spaced vertical rods fixedly mounted on the support plate, each vertical rod being fixedly connected to a limit ring, and a drive mechanism mounted on the housing.

[0008] The housing contains a first container placed on a flexible pad. The upper end of the first container extends outward. The upper end of the vertical rod passes through the upper end of the first container. The limiting ring is located below the upper end of the first container. A ring frame is fixedly installed at the lower end of the first container. The first container contains two sets of mirror-distributed limiting mechanisms. A cover for sealing the upper port of the housing is provided on the top of the housing. The cover is threaded into the upper port of the housing.

[0009] Preferably, the limiting mechanism includes a vertical plate fixedly installed at the bottom of the housing, a horizontally slidable baffle passing through the vertical plate, elastic elements fixedly connected to both sides of the baffle, one end of the elastic element being fixed to the vertical plate, and an inclined plate fixedly installed on the baffle.

[0010] An annular sleeve is fixedly installed at the bottom of the cap, and the annular sleeve covers the outside of the first container.

[0011] Preferably, the drive mechanism includes a servo motor fixedly mounted on the housing, and the output shaft of the servo motor is fixedly connected to the rotating shaft.

[0012] Preferably, the housing has multiple heat dissipation holes, one end of which extends to the servo motor.

[0013] Preferably, a handle is fixedly installed on the top of the cover.

[0014] Preferably, an annular component is fixedly installed inside the first container, and a second container is provided inside the first container. The upper end of the second container extends outward and is threadedly connected to the inner wall of the first container. The annular component is located below the upper end of the second container. The bottom of the second container is open, and the second container is filled with a hydrophilic column.

[0015] Preferably, a sealing plug is provided at the upper port of the second container, and the lower end of the sealing plug is threaded into the second container and used to seal the upper port of the second container.

[0016] Preferably, the upper end of the first container is provided with multiple pressure balancing ports, and the inner cavity of the first container is connected to the outside through the pressure balancing ports.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0018] The protein centrifugation extraction device of this application limits the vertical movement of the first container, which can prevent the first container from shaking up and down, prevent the first container from breaking and the equipment from being damaged, thereby improving experimental safety, avoiding the spread of biological contamination, and eliminating centrifugation accidents.

[0019] After centrifugation, the cap is removed, the annular sleeve moves upward and completely separates from the two inclined plates, and the elastic force of the elastic element drives the baffle to reset and move away from above the annular frame, allowing the first container to be quickly removed. The limiting and unlimiting operations of the first container can be automatically completed through the cap installation and removal steps, simplifying the operation. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of a protein centrifugation extraction device proposed in this utility model;

[0021] Figure 2 This is a schematic diagram of the internal structure of the shell in a protein centrifugation extraction device proposed in this utility model;

[0022] Figure 3 This is a cross-sectional view of a protein centrifugation extraction device proposed in this utility model;

[0023] Figure 4 This is an exploded view of the shell and servo motor in a protein centrifugation extraction device proposed in this utility model.

[0024] Figure 5 This is an enlarged cross-sectional view of a portion of the structure of the first container in a protein centrifugation extraction device proposed in this utility model;

[0025] Figure 6 This is an enlarged schematic diagram of a portion of the annular sleeve in a protein centrifugal extraction device proposed in this utility model.

[0026] In the diagram: 1. Shell; 2. Shaft; 3. Support plate; 4. Flexible pad; 5. Vertical rod; 6. Limiting ring; 7. First container; 8. Ring frame; 9. Cover; 10. Vertical plate; 11. Baffle; 12. Elastic element; 13. Inclined plate; 14. Ring sleeve; 15. Servo motor; 16. Heat dissipation hole; 17. Ring element; 18. Second container; 19. Hydrophilic column; 20. Sealing plug; 21. Pressure balance port. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0028] Example 1

[0029] Reference Figure 1-6A protein centrifugation extraction device includes a housing 1, a rotating shaft 2 rotatably mounted at the bottom of the housing 1, a support plate 3 fixedly mounted on the top of the rotating shaft 2, a flexible pad 4 fixedly connected to the top of the support plate 3, two spaced vertical rods 5 fixedly mounted on the support plate 3, each vertical rod 5 fixedly connected to a limit ring 6, and a drive mechanism mounted on the housing 1.

[0030] The drive mechanism includes a servo motor 15 fixedly mounted on the housing 1, with the output shaft of the servo motor 15 fixedly connected to the rotating shaft 2. The servo motor 15 drives the rotating shaft 2 to rotate, which in turn drives the support plate 3 to rotate. Multiple heat dissipation holes 16 are provided on the housing 1, with one end of each hole extending to the servo motor 15. These heat dissipation holes 16 are used to dissipate heat from the servo motor 15.

[0031] The housing 1 contains a first container 7 placed on a flexible pad 4. The flexible pad 4 is positioned between the first container 7 and the support plate 3, and the flexible pad 4 can protect the bottom of the first container 7. The upper end of the first container 7 extends outward, and the upper end of the vertical rod 5 passes through the upper end of the first container 7. The limiting ring 6 is located below the upper end of the first container 7. The lower end of the first container 7 is fixedly installed with a ring frame 8. The first container 7 contains two sets of mirror-distributed limiting mechanisms. The housing 1 is provided with a cover 9 for sealing the upper port of the housing 1. The cover 9 is threadedly connected to the upper port of the housing 1, and a handle is fixedly installed on the top of the cover 9.

[0032] The limiting mechanism includes a vertical plate 10 fixedly installed at the bottom of the housing 1, a horizontally slidable baffle 11 passing through the vertical plate 10, elastic elements 12 fixedly connected to both sides of the baffle 11, one end of the elastic element 12 fixed to the vertical plate 10, and an inclined plate 13 fixedly installed on the baffle 11. An annular sleeve 14 is fixedly installed at the bottom of the cover 9, covering the first container 7.

[0033] In this embodiment, the first container 7 holds the crude protein extract. The first container 7 is placed inside the shell 1, with the upper end of the vertical rod 5 passing through the upper end of the first container 7. The lower part of the first container 7 is limited by the limiting ring 6. The cap 9 is screwed into the upper port of the shell 1. During the screwing process, the cap 9 rotates and moves downward. The annular sleeve 14 on the cap 9 moves down and contacts the inclined plates 13 on both sides. The annular sleeve 14 can push the inclined plates 13 on both sides closer to each other. The inclined plates 13 and the baffle 11 move synchronously, and the elastic element 12 contracts. After the cap 9 is installed, the baffle 11 moves above the annular frame 8 and limits the upper part of the annular frame 8. The annular frame 8 is connected to the first container 7 and the position remains consistent. Thus, the upper and lower parts of the first container 7 are limited by the baffle 11 and the limiting ring 6, preventing the first container 7 from shaking up and down. The protein centrifugation extraction device of this application limits the vertical movement of the first container 7, which can prevent the first container 7 from shaking up and down, prevent the first container 7 from breaking and the equipment from being damaged, thereby improving experimental safety, avoiding the spread of biological contamination, and eliminating centrifugation accidents.

[0034] During the rotation of the support plate 3, the first container 7 is driven to rotate synchronously through the vertical rod 5. The high-speed rotation of the first container 7 and the resulting strong centrifugal force achieve separation based on the differences in density and sedimentation coefficient between the protein and other components.

[0035] After centrifugation, the cap 9 is removed, the annular sleeve 14 moves upward and completely separates from the two inclined plates 13, and the elastic force of the elastic element 12 drives the baffle 11 to reset and move away from above the annular frame 8, allowing the first container 7 to be quickly removed. The limiting and unlimiting operations of the first container 7 can be automatically completed through the installation and removal steps of the cap 9, simplifying the operation steps.

[0036] Based on Example 1, Example 2:

[0037] Reference Figure 2-6 An annular component 17 is fixedly installed inside the first container 7. A second container 18 is located inside the first container 7, with its upper end extending outwards and threadedly connected to the inner wall of the first container 7. The annular component 17 is located below the upper end of the second container 18. The bottom of the second container 18 is open, and it is filled with a hydrophilic column 19. A sealing plug 20 is provided at the upper port of the second container 18, with its lower end threaded into the second container 18 and used to seal the upper port. Multiple pressure balancing ports 21 are provided at the upper end of the first container 7, and the inner cavity of the first container 7 communicates with the outside through these ports. The pressure balancing ports 21 are used to balance the pressure inside the first container 7.

[0038] In this embodiment, the crude protein extract is placed in the second container 18. During centrifugation, the second container 18 rotates synchronously with the first container 7. The rotation speed of the second container 18 is related to the required rotation speed of the protein solution to be separated, and since different protein solutions have different rotation speed requirements, it is not limited. During centrifugation, because the hydrophilic column 19 inside the second container 18 is made of hydrophilic material, the protein solution can pass through while retaining column lipids, insoluble matter, and cell debris. Therefore, the retained column lipids, insoluble matter, and cell debris remain in the second container 18. Under the action of centrifugal force, the protein solution can pass through the hydrophilic column 19 and enter the first container 7 from the lower port of the second container 18. After centrifugation, the second container 18 is rotated, removed, and discarded. The protein solution in the first container 7 is then aspirated using a pipette to complete the centrifugal extraction of protein. Through the above separation method, the protein solution is less susceptible to contamination, has high purity, and can be directly separated after centrifugation, facilitating pipetting.

[0039] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A protein centrifugal extraction device, comprising a housing (1), characterized in that, The bottom of the housing (1) is rotatably mounted with a rotating shaft (2), the top of the rotating shaft (2) is fixedly mounted with a support plate (3), the top of the support plate (3) is fixedly connected with a flexible pad (4), two spaced vertical rods (5) are fixedly mounted on the support plate (3), and each vertical rod (5) is fixedly connected with a limit ring (6). The housing (1) is equipped with a driving mechanism. The housing (1) contains a first container (7) placed on a flexible pad (4). The upper end of the first container (7) extends outward. The upper end of the vertical rod (5) passes through the upper end of the first container (7). The limiting ring (6) is located below the upper end of the first container (7). A ring frame (8) is fixedly installed at the lower end of the first container (7). Two sets of mirror-distributed limiting mechanisms are provided inside the first container (7). A cover (9) for sealing the upper port of the housing (1) is provided on the upper part of the housing (1). The cover (9) is threadedly connected to the upper port of the housing (1).

2. The protein centrifugation extraction apparatus according to claim 1, characterized in that, The limiting mechanism includes a vertical plate (10) fixedly installed at the bottom of the housing (1), a horizontally sliding baffle (11) passing through the vertical plate (10), elastic elements (12) fixedly connected to both sides of the baffle (11), one end of the elastic element (12) fixed to the vertical plate (10), and an inclined plate (13) fixedly installed on the baffle (11). The bottom of the cover (9) is fixedly fitted with an annular sleeve (14), which covers the outside of the first container (7).

3. The protein centrifugation extraction apparatus according to claim 1, characterized in that, The drive mechanism includes a servo motor (15) fixedly mounted on the housing (1), and the output shaft of the servo motor (15) is fixedly connected to the rotating shaft (2).

4. The protein centrifugal extraction apparatus according to claim 3, characterized in that, The housing (1) has multiple heat dissipation holes (16), one end of which extends to the servo motor (15).

5. The protein centrifugation extraction apparatus according to claim 1, characterized in that, A handle is fixedly installed on the top of the cover (9).

6. The protein centrifugation extraction apparatus according to claim 1, characterized in that, An annular component (17) is fixedly installed inside the first container (7). A second container (18) is provided inside the first container (7). The upper end of the second container (18) extends outward and is threadedly connected to the inner wall of the first container (7). The annular component (17) is located below the upper end of the second container (18). The bottom of the second container (18) is open. The second container (18) is filled with a hydrophilic column (19).

7. The protein centrifugal extraction apparatus according to claim 6, characterized in that, A sealing plug (20) is provided at the upper port of the second container (18). The lower end of the sealing plug (20) is threaded into the second container (18) and is used to seal the upper port of the second container (18).

8. The protein centrifugation extraction apparatus according to claim 7, characterized in that, The first container (7) has multiple pressure balance ports (21) at its upper end, and the inner cavity of the first container (7) is connected to the outside through the pressure balance ports (21).

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