Integrated CUMS reaction box

Abstract

An integrated CUMS reaction box comprises a box body, at least two spaces for independently bearing mouse cages are arranged in the box body, laboratory mice are arranged in the mouse cages, a box door is movably connected to the side face of the box body, and each space of the box body is correspondingly provided with a lighting mechanism, a sound production mechanism, a heating mechanism, a support mechanism and a water supply mechanism which are used for the mouse cages. The lighting mechanism, the sound production mechanism, the heating mechanism, the support mechanism and the water supply mechanism are integrated in a single box body, various pressure stimulations can be performed on laboratory mice in a mouse cage of the single box body, on one hand, various independent experiment devices are integrated, and the situation that a large space is occupied during an experiment is avoided; 2, the physical stress reaction of the experimental mouse is prevented from being increased by transferring the experimental mouse, and the operation complexity is reduced; and thirdly, variable parameters in the box body are convenient to adjust, the accuracy of parameter regulation and control is improved, and the consistency of experimental results is improved.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment technology, and in particular to an integrated CUMS reaction chamber. Background Technology

[0002] CUMS is an abbreviation for "Chronic Unpredictable Mild Stress." This model is used to study animal depression by exposing them to a series of unpredictable mild stress stimuli over a prolonged period. The main animal subjects are 4-week-old, 14-16g C57BL / 6 mice (before the experiment, the mice should be placed in separate ventilated cages with humidity ranging from 40% to 50% and temperature at 24±1°C, with a 12-hour diurnal cycle, and used for the experiment after one week).

[0003] The various stress stimuli experienced by mice in the chronic unpredictable mild stress model include:

[0004] First, withhold food and water for 24 hours, that is, remove food and water from the rat cage for 24 hours.

[0005] Secondly, heat stress (40°C, 5 min), that is, setting the temperature in the rat cage to 40°C and exposing it to the constant temperature for 5 minutes;

[0006] Third, clamp the tail for 2 minutes, that is, gently clamp the tail of the experimental mouse with hemostatic forceps for 2 minutes.

[0007] Fourth, 24-hour strobe light, which means placing a strobe light above the rat cage and keeping it on for 24 hours.

[0008] Fifth, tilt cage (45°, 24 h), that is, tilt the cage at a 45-degree angle for 24 hours.

[0009] Sixth, empty bottle 24h, that is, providing mice with food and empty water bottles for 24 hours continuously;

[0010] Seventh, the 6-hour crowding test involves placing multiple irregular objects into the rat cage, compressing the normal living space of the experimental rats for 6 hours.

[0011] Eighth, the day and night are reversed 24 hours, that is, the current lighting conditions are changed in 12-hour cycles. In one cycle, 12 hours are light (daytime) and the next 12 hours are darkness (nighttime).

[0012] Ninth, damp straw mat (200ml, 24h): add 200ml of water to the straw mat in the rat cage and leave it for 24 hours.

[0013] Tenth, ice water swimming (4°C, 5 min): Place the mouse cage in ice water at about 4°C, with a volume of water that allows the mouse to float and swim freely, and let it swim for 5 minutes.

[0014] Eleventh, noise stimulation, namely, playing high-decibel noise of 50kb-70kb for 24 hours.

[0015] The various stress stimuli were numbered sequentially and randomly applied using a random number table, with no single stimulus being administered for five consecutive days within a four-week period. Furthermore, the mice were ensured not to be exposed to the same stress stimulus for more than three consecutive days to prevent them from anticipating the occurrence of specific stress stimuli.

[0016] Establishing the aforementioned chronic unpredictable mild stress model requires the alternating use of various independent experimental devices that generate different stress stimuli, such as the restraint heat stress experimental device (application number 202411159752.2), the noise-induced modeling cage (application number 202420121420.4), or the mouse restraint device (application number 202323014069.3). However, using these devices and applying various stress stimuli has the following drawbacks:

[0017] First, it occupies a lot of space: the various independent experimental devices occupy a large amount of laboratory space;

[0018] Secondly, the operation is cumbersome and increases the stress response of the experimental mice: the experimental mice need to be repeatedly transferred according to various stress stimuli, which is cumbersome and increases the stress response of the experimental mice.

[0019] Third, poor variable control: Various variables exist in various independent experimental devices, and the precision of variable parameter control is poor, which affects the experimental results.

[0020] Based on this, an integrated CUMS reaction chamber was designed, which reduces the space occupied by experimental equipment, avoids frequent transfer of mice and stress response, and enables precise control of various variables. Utility Model Content

[0021] The present invention aims to overcome the defects in the prior art and provide an integrated CUMS reaction chamber.

[0022] To achieve the above objectives, the technical solution adopted by this utility model is: an integrated CUMS reaction chamber, including a chamber body, the chamber body having at least two independent spaces for holding mouse cages, each mouse cage containing an experimental mouse, and a door movably connected to the side of the chamber body to open or close the space, each space of the chamber body being correspondingly provided with a lighting mechanism for illuminating the mouse cage, a sound-generating mechanism for generating different decibels in the mouse cage, a heating mechanism for heating the mouse cage, a support mechanism for tilting the mouse cage, and a water supply mechanism for supplying water to the inside of the mouse cage, wherein the lighting mechanism, sound-generating mechanism, heating mechanism, support mechanism, and water supply mechanism can be used selectively or in combination.

[0023] In a preferred embodiment of this utility model, the lighting mechanism is located at the top of the box, the heating mechanism is located at the bottom of the box, the sound-generating mechanism and the water supply mechanism are respectively located on different sides of the box, and the support mechanism is located inside the box and corresponds to the slot at the bottom of the box.

[0024] As a preferred embodiment of this utility model, the box body also has at least one slot, and the slot is provided with a movable plate that can separate two adjacent spaces, and each space is the same size.

[0025] As a preferred embodiment of this utility model, the support mechanism includes a rotating plate, a support plate, a rotating plate movable component, and a support plate movable component. The rotating plate and the support plate are arranged opposite to each other on both sides of the slot. The support plate is rotatably connected to the inner wall of the bottom of the box through the support plate movable component. One end of the rotating plate is rotatably connected to the inner wall of the bottom of the box through the rotating plate movable component. The other end of the rotating plate has a plurality of slots along its length that allow the support plate to be inserted. The support plate is inserted into different slots so that the rotating plate and the bottom of the box form different angles.

[0026] As a preferred embodiment of this utility model, the rotating plate is provided with at least two positioning parts at one end facing the support plate, and the bottom of the box body is connected to the slot with a positioning groove that can support the positioning parts. When the positioning part is located in the positioning groove, the rotating plate is laid flat on the bottom of the box body and located in the slot. The rotating plate is also provided with silicone to prevent the mouse cage from sliding on the side facing the mouse cage.

[0027] As a preferred embodiment of this utility model, the sound-generating mechanism includes an outer shell, an inner shell, and a sound-generating component housed inside the outer shell and the inner shell. The outer shell and the inner shell are fixedly connected, and at least one of the outer shell and the inner shell is fixed to the side of the enclosure. Both the outer shell and the inner shell are provided with a plurality of through holes that allow sound to pass into the interior of the enclosure.

[0028] As a preferred embodiment of this utility model, the heating mechanism includes a heating shell, a heating wire located inside the heating shell, and a temperature sensor near the heating wire. The heating shell is fixedly installed at the bottom of the box.

[0029] As a preferred embodiment of this utility model, the water supply mechanism includes a water inlet and a water pipe passing through the water inlet and capable of injecting water into the rat cage. The water inlet connects the inside and outside of the cage.

[0030] As a preferred embodiment of the present invention, the lighting mechanism includes a lighting housing, a fluorescent lamp and a strobe lamp, wherein the fluorescent lamp and the strobe lamp are fixedly disposed inside the lighting housing, and the lighting housing is located at the top center of the space.

[0031] As a preferred embodiment of this utility model, it includes a PLC controller, which is electrically connected to a fluorescent lamp, a strobe lamp, a sound-generating element, a heating wire, and a temperature sensor via wires.

[0032] The beneficial effects of this utility model are:

[0033] The lighting mechanism, sound generation mechanism, heating mechanism, support mechanism, and water supply mechanism of this utility model are integrated into a single box, and can apply various pressure stimuli to experimental mice in a single box cage. Firstly, it integrates multiple independent experimental devices, avoiding the need to occupy a lot of space during experiments; secondly, it avoids increasing the stress response of experimental mice by transferring them, reducing the complexity of operation; and thirdly, the variable parameters inside the box are easy to adjust, improving the accuracy of parameter control and the consistency of experimental results. Attached Figure Description

[0034] Figure 1 This is a schematic diagram of the structure of this utility model;

[0035] Figure 2 This is a schematic diagram of the internal structure of this utility model;

[0036] Figure 3 This is a schematic diagram of the sound-generating mechanism of this utility model;

[0037] Figure 4 This is a schematic diagram of the lighting mechanism of this utility model;

[0038] Figure 5 This is a partial sectional view of the interior of the heating mechanism of this utility model;

[0039] The attached diagram shows the following reference numerals: 1. Housing, 2. Lighting mechanism, 3. Sound-generating mechanism, 4. Heating mechanism, 5. Support mechanism, 6. Water supply mechanism, 7. Movable plate, 8. PLC controller, 11. Housing door, 12. Slot, 13. Slot, 21. Lighting housing, 22. Fluorescent lamp, 23. Strobe lamp, 31. Outer housing, 32. Inner housing, 33. Sound-generating component, 41. Heating housing, 42. Heating wire, 43. Temperature sensor, 51. Rotating plate, 52. Support plate, 53. Rotating plate movable component, 54. Support plate movable component, 61. Water interface, 62. Water pipe, 100. Space, 121. Positioning slot, 511. Socket, 512. Positioning part, 513. Silicone. Detailed Implementation

[0040] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0041] like Figures 1-5 As shown, an integrated CUMS reaction chamber includes a chamber body 1. The chamber body 1 has at least two independent spaces 100 for holding mouse cages. The mouse cages are equipped with experimental mice. The side of the chamber body 1 is movably connected to a door 11 for opening or closing the spaces 100. Each space 100 of the chamber body 1 is correspondingly provided with a lighting mechanism 2 for illuminating the mouse cage, a sound-generating mechanism 3 for generating different decibels in the mouse cage, a heating mechanism 4 for heating the mouse cage, a support mechanism 5 for tilting the mouse cage, and a water supply mechanism 6 for supplying water to the inside of the mouse cage. The lighting mechanism 2, the sound-generating mechanism 3, the heating mechanism 4, the support mechanism 5, and the water supply mechanism 6 can be used individually or in combination.

[0042] The length of the housing 1 is preferably 600mm, the width is preferably 500mm, and the height is preferably 310mm. The inner wall of the housing 1 is covered with a 1.5mm thick polycarbonate insulation layer.

[0043] The cages used to house laboratory mice are general-purpose components, with the m1 cage being the preferred choice.

[0044] The box 1 also has at least one slot 13 inside, and the slot 13 is provided with a movable plate 7 that can separate two adjacent spaces 100. The movable plate 7 is preferably made of polytetrafluoroethylene. Each space 100 separated by the movable plate 7 is the same size and can be used for independent experiments.

[0045] Specifically, several independent spaces 100 are set up through the activity board 7, and experimental group mice and control group mice are set up in different spaces 100 to support simultaneous experiments of chronic unpredictable mild stress model group and control group, thereby improving experimental efficiency, accuracy and authenticity.

[0046] The lighting mechanism 2 is located at the top of the box 1, the heating mechanism 4 is located at the bottom of the box 1, the sound-emitting mechanism 3 and the water supply mechanism 6 are respectively located on different sides of the box 1, and the support mechanism 5 is located inside the box 1 and corresponds to the slot 12 at the bottom of the box. The specific structures of the lighting mechanism 2, the sound-emitting mechanism 3, the heating mechanism 4, the support mechanism 5 and the water supply mechanism 6 are as follows, which facilitates various pressure stimuli to the experimental mice in the cage.

[0047] The support mechanism 5 includes a rotating plate 51, a support plate 52, a rotating plate movable part 53, and a support plate movable part 54. The rotating plate 51 and the support plate 52 are arranged opposite to each other on both sides of the slot 12. The support plate 52 is rotatably connected to the bottom inner wall of the box 1 through the support plate movable part 54. One end of the rotating plate 51 is rotatably connected to the bottom inner wall of the box 1 through the rotating plate movable part 53. The other end of the rotating plate 51 has a plurality of insertion slots 511 along its length direction, which allow the support plate 52 to be inserted. The support plate 52 is inserted into different insertion slots 511 so that the rotating plate 51 and the bottom of the box 1 are at different angles.

[0048] The rotating plate movable part 53 and the support plate movable part 54 are preferably hinges.

[0049] At least two positioning parts 512 are provided at one end of the rotating plate 51 facing the support plate 52. The bottom of the box 1 is connected to the slot 12 and has a positioning groove 121 that can support the positioning parts 512. When the positioning part 512 is located in the positioning groove 121, the rotating plate 51 is located in the slot 12 and its upper surface is flush with the inner wall of the bottom of the box 1. The side of the rotating plate 51 facing the mouse cage is also provided with silicone 513 to prevent the mouse cage from sliding.

[0050] Specifically, the support plate 52 is inserted into the designated socket 511, so that the rotating plate 51 is at a designated angle to the bottom of the box 1. Correspondingly, the mouse cage located on the rotating plate 51 is also at a designated angle. Furthermore, the rotating plate 51 is provided with silicone 513 to increase friction, so that the mouse cage can be fixed at a designated position on the rotating plate 51, thereby completing the tilt cage pressure stimulation.

[0051] After the tilt cage pressure stimulation is completed, the support plate 52 is removed, and the positioning part 512 on the rotating plate 51 is placed in the positioning groove 121. The upper surface of the rotating plate 51 is flush with the inner wall of the bottom of the box 1, and can be used to place the mouse cage.

[0052] The sound-generating mechanism 3 includes an outer shell 31, an inner shell 32, and a sound-generating element 33 housed inside the outer shell 31 and the inner shell 32. The outer shell 31 and the inner shell 32 are fixedly connected, and at least one of the outer shell 31 and the inner shell 32 is fixed to the side of the enclosure 1. Both the outer shell 31 and the inner shell 32 are provided with a plurality of through holes that allow sound to pass into the interior of the enclosure 1.

[0053] Specifically, after the sound-generating element 33 is placed between the outer shell 31 and the inner shell 32, the two are connected by means of snap-fit ​​or threaded connection. Then, the sound-generating mechanism 3 is threaded to the box 1 by the connectors on the outer shell 31 and / or the inner shell 32. The sound-generating element 33 is controlled by the PLC controller to produce sound, thereby generating noise for the experimental mice in the cage. The noise intensity of the sound-generating element 33 is adjustable, ranging from 50 to 90 dB, thus completing the noise pressure stimulation.

[0054] The entire sound-generating mechanism 3 can also be removed from the side of the enclosure 1 to ensure air circulation inside and outside the enclosure 1.

[0055] The heating mechanism 4 includes a heating housing 41, a heating wire 42 located inside the heating housing 41, and a temperature sensor 43 near the heating wire 42. The temperature sensor 43 is preferably a thermocouple. The heating housing 41 is fixedly installed at the bottom of the box 1. The connection between the heating housing 41 and the box 1 can be welding or threaded connection, etc.

[0056] Specifically, the heating wire 42 inside the heating housing 41 is heated by the PLC controller. At the same time, the temperature sensor 43 located inside the heating housing 41 can collect the temperature of the heating wire 42 and convert it into a signal that can be recognized by the PLC. The PLC controller automatically adjusts the power output of the heating wire 42 according to the deviation between the real-time temperature value fed back by the temperature sensor 43 and the set target temperature value, thereby achieving precise temperature control. Correspondingly, the heating mechanism 4 can heat the bottom of the box 1, thereby completing the thermal stress pressure stimulation.

[0057] The water supply mechanism 6 includes a water interface 61 and a water pipe 62 that passes through the water interface 61 and can inject water into the rat cage. The water interface 61 connects the inside and outside of the box 1.

[0058] Specifically, the amount of water in the water pipe 62 is controllable. On the one hand, an appropriate amount of bedding is laid in the rat cage, and a certain amount of water is sprayed into the bedding in the rat cage through the water pipe 62, thereby completing the pressure stimulation of the wet straw mat. On the other hand, if no bedding is laid in the rat cage, the temperature of the water in the water pipe 62 is controlled, and a certain amount of water that can make the experimental rat float and swim freely is injected into the rat cage, thereby completing the pressure stimulation of ice water swimming.

[0059] The lighting mechanism 2 includes a lighting housing 21, a fluorescent lamp 22 and a strobe lamp 23. The fluorescent lamp 22 and the strobe lamp 23 are fixedly installed inside the lighting housing 21, which is located at the top center of the space 100.

[0060] Specifically, the PLC controller can customize the switching time of the fluorescent lamp 22 to simulate day and night, thereby completing a 24-hour pressure stimulation with day and night reversed; the PLC controller controls the frequency of the strobe lamp 23, thereby completing a 24-hour strobe pressure stimulation.

[0061] It should be noted that the frequency of the strobe lamp 23 in this utility model is adjustable from 0.1 to 100 Hz, and the standard stress parameter is set to a 10 Hz square wave pulse (duty cycle 50%).

[0062] The fluorescent lamp 22, strobe lamp 23, sound-emitting element 33, heating wire 42, and temperature sensor 43 are electrically connected to an external PLC controller via wires, thereby enabling the PLC controller to control the fluorescent lamp 22, strobe lamp 23, sound-emitting element 33, heating wire 42, and temperature sensor 43 in real time.

[0063] Other stress stimuli, such as fasting and water deprivation for 24 hours, can be achieved simply by placing the cage containing the experimental mice in a space of 100, removing the food and water bottles from the cage, and continuing this for 24 hours.

[0064] For example, to perform a 2-minute tail clamping, simply place the cage containing the experimental mouse in space 100, open the box door 11, and gently clamp the tail with hemostatic forceps for 2 minutes to complete the 2-minute tail clamping pressure stimulation.

[0065] For example, for 24 hours of empty bottle stress stimulation, simply place the cage containing the experimental mouse in a space of 100, provide food and an empty water bottle, and continue for 24 hours to complete the 24-hour empty bottle stress stimulation.

[0066] For example, to achieve 6 hours of crowding, place the cage containing the experimental mice in a space of 100 and put multiple irregular objects into the cage to compress the mice's normal living space for 6 hours, thus completing the 6-hour crowding stress stimulation.

[0067] The lighting mechanism, sound generation mechanism, heating mechanism, support mechanism, and water supply mechanism of this utility model are integrated into a single box, and can apply various pressure stimuli to experimental mice in a single box cage. Firstly, it integrates multiple independent experimental devices, avoiding the need to occupy a lot of space during experiments; secondly, it avoids increasing the stress response of experimental mice by transferring them, reducing the complexity of operation; and thirdly, the variable parameters inside the box are easy to adjust, improving the accuracy of parameter control and the consistency of experimental results.

[0068] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention; therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0069] Although this document frequently uses reference numerals from the accompanying drawings: 1. housing, 2. lighting mechanism, 3. sound-generating mechanism, 4. heating mechanism, 5. support mechanism, 6. water supply mechanism, 7. movable plate, 8. PLC controller, 11. housing door, 12. slot, 13. slot, 21. lighting housing, 22. fluorescent lamp, 23. strobe lamp, 31. outer housing, 32. inner housing, 33. sound-generating element, 41. heating housing, 42. heating wire, 43. temperature sensor, 51. rotating plate, 52. support plate, 53. rotating plate movable part, 54. support plate movable part, 61. water interface, 62. water pipe, 100. space, 121. positioning groove, 511. socket, 512. positioning part, 513. silicone, etc., the possibility of using other terms is not excluded; these terms are used merely for the convenience of describing and explaining the essence of this utility model; interpreting them as any additional limitation would be contrary to the spirit of this utility model.

Claims

1. An integrated CUMS reaction chamber, characterized in that: The enclosure includes a box (1), which has at least two independent spaces (100) for carrying mouse cages. Each mouse cage contains an experimental mouse. The side of the box (1) is movably connected to a door (11) for opening or closing the spaces (100). Each space (100) of the box (1) is provided with a lighting mechanism (2) for illuminating the mouse cage, a sound-generating mechanism (3) for generating different decibels in the mouse cage, a heating mechanism (4) for heating the mouse cage, a support mechanism (5) for tilting the mouse cage, and a water supply mechanism (6) for supplying water to the inside of the mouse cage. The lighting mechanism (2), the sound-generating mechanism (3), the heating mechanism (4), the support mechanism (5), and the water supply mechanism (6) can be used individually or in combination.

2. The integrated CUMS reaction chamber according to claim 1, characterized in that: The lighting mechanism (2) is located on the top of the box (1), the heating mechanism (4) is located on the bottom of the box (1), the sound-generating mechanism (3) and the water supply mechanism (6) are respectively located on different sides of the box (1), and the support mechanism (5) is located inside the box (1) and corresponds to the slot (12) at the bottom of the box.

3. The integrated CUMS reaction chamber according to claim 1, characterized in that: The box (1) also has at least one slot (13) inside, and the slot (13) is provided with a movable plate (7) that can separate two adjacent spaces (100), and each space (100) is the same size.

4. An integrated CUMS reaction chamber according to claim 2, characterized in that: The support mechanism (5) includes a rotating plate (51), a support plate (52), a rotating plate movable part (53), and a support plate movable part (54). The rotating plate (51) and the support plate (52) are arranged opposite to each other on both sides of the slot (12). The support plate (52) is rotatably connected to the bottom inner wall of the box (1) through the support plate movable part (54). One end of the rotating plate (51) is rotatably connected to the bottom inner wall of the box (1) through the rotating plate movable part (53). The other end of the rotating plate (51) has several slots (511) along its length direction that allow the support plate (52) to be inserted. The support plate (52) is inserted into different slots (511) so that the rotating plate (51) and the bottom of the box (1) are at different angles.

5. An integrated CUMS reaction chamber according to claim 4, characterized in that: The rotating plate (51) is provided with at least two positioning parts (512) at one end facing the support plate (52). The bottom of the box (1) is connected to the slot (12) and has a positioning groove (131) that can support the positioning parts (512). When the positioning part (512) is located in the positioning groove (131), the rotating plate (51) is laid flat on the bottom of the box (1) and located in the slot (12). The rotating plate (51) is also provided with silicone (513) to prevent the mouse cage from sliding on the side facing the mouse cage.

6. An integrated CUMS reaction chamber according to claim 2, characterized in that: The sound-generating mechanism (3) includes an outer shell (31), an inner shell (32), and a sound-generating element (33) housed inside the outer shell (31) and the inner shell (32). The outer shell (31) and the inner shell (32) are fixedly connected. At least one of the outer shell (31) and the inner shell (32) is fixed to the side of the box (1). The outer shell (31) and the inner shell (32) are each provided with a number of through holes that can allow sound to pass into the box (1).

7. An integrated CUMS reaction chamber according to claim 2, characterized in that: The heating mechanism (4) includes a heating housing (41), a heating wire (42) located inside the heating housing (41), and a temperature sensor (43) near the heating wire (42). The heating housing (41) is fixedly installed at the bottom of the box (1).

8. An integrated CUMS reaction chamber according to claim 2, characterized in that: The water supply mechanism (6) includes a water interface (61) and a water pipe (62) that passes through the water interface (61) and can inject water into the rat cage. The water interface (61) connects the inside and outside of the box (1).

9. An integrated CUMS reaction chamber according to claim 2, characterized in that: The lighting mechanism (2) includes a lighting housing (21), a fluorescent lamp (22) and a strobe lamp (23). The fluorescent lamp (22) and the strobe lamp (23) are fixedly installed inside the lighting housing (21), which is located at the top center of the space (100).

10. An integrated CUMS reaction chamber according to claim 1, characterized in that: The system includes a PLC controller (8), which is electrically connected to a fluorescent lamp (22), a strobe lamp (23), a sound generator (33), a heating wire (42), and a temperature sensor (43) via wires.

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