Auxiliary tool for cell scratch experiment

Abstract

The utility model relates to an auxiliary tool for a cell scratch experiment, which comprises a fixed base, a sliding seat and a scratch pen, the fixed base is portal frame-shaped, one end of the fixed base is screwed with an adjusting bolt I for fixing a cell culture pore plate, and the top of the fixed base is provided with a strip-shaped hole extending along the long axis direction of the fixed base; the sliding seat is slidably mounted at the top end of the fixed base, and the sliding seat linearly slides in the long axis direction of the fixed base; the scratching pen is installed in the center of the sliding seat, and the bottom of the scratching pen is inserted into the strip-shaped hole from top to bottom. The device has the beneficial effects that the adjusting bolt and the fixed base are matched to clamp the cell culture pore plate, and the sliding seat is slidably mounted at the top end of the fixed base to support and guide the scratching pen, so that the scratching pen can stably move in the same straight line direction during scratching operation, and the scratching pen is favorably ensured to be a straight line with uniform width; and the scratch quality is ensured.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to an auxiliary tool for cell scratch experiments. Background Technology

[0002] The cell scratch assay is a simple and intuitive in vitro experimental method that studies cell migration capabilities and the mechanisms of cell-cell interactions based on cell migration characteristics. This assay simulates the migration process of cells after injury and is often used to assess the effects of drugs, genes, or environmental factors on cell migration. The typical method involves artificially creating a cell-free region (i.e., a scratch) on a cell monolayer cultured in vitro, and then placing the cell culture plate in an incubator for further culture. Under suitable environmental conditions, cells around the scratch will migrate towards the scratched area to fill this cell-free region. By observing cell migration in the scratched area at different time points, the cell migration ability can be assessed.

[0003] To create a cell-free area (i.e., a scratch) on a monolayer of cells, a common method is to use a ruler as a guide and gravitate the pipette tip along the ruler to scratch the monolayer of cells in the cell culture plate. Since the scratching process relies on the operator's hands, shaking of the ruler and pipette tip can easily cause the scratch to become distorted, and different pipette tip angles can result in uneven scratch widths, affecting the quality of the scratch. Utility Model Content

[0004] The purpose of this invention is to overcome the above-mentioned problems in the existing technology and provide an auxiliary tool for cell scratch experiments.

[0005] To achieve the above-mentioned technical objectives and effects, this utility model is implemented through the following technical solution:

[0006] A cell scratch assay tool includes:

[0007] A fixed base, which is gantry-shaped, is screwed to one end of the fixed base with an adjusting bolt for fixing the cell culture plate, and the top of the fixed base has a strip-shaped hole extending along the long axis of the fixed base.

[0008] A sliding seat is slidably mounted on the top of a fixed base, and the sliding seat slides in a straight line along the long axis of the fixed base;

[0009] A scratching pen, which is mounted at the center of a sliding base, with its bottom inserted into a strip-shaped hole from top to bottom.

[0010] Each of the fixed bases at both ends of the strip-shaped hole is provided with an upwardly protruding indicator block.

[0011] The fixed base has a screw hole at one end that is parallel to the long axis of the fixed base, and the adjusting bolt is screwed into the screw hole.

[0012] Preferably, the tail of the adjusting bolt is fitted with a rubber protective sleeve for abutting against the side of the cell culture plate.

[0013] The fixed base has two grooves on its top that are parallel to the strip-shaped hole, and the bottom of the sliding seat has two parallel sliders fixedly connected to it. The sliders and the grooves are slidably engaged in a one-to-one correspondence.

[0014] The slider has a through-hole at its center, and the scratch pen is installed in the hole. The sliding seat has a screw hole two perpendicular to the hole on one side, and an adjusting bolt for fixing the scratch pen is screwed into the screw hole two.

[0015] The scratching pen includes a cylindrical pen barrel and a circular rubber ring. A scratching needle coaxial with the pen barrel is fixed to the bottom end of the pen barrel. The rubber ring is sleeved on the pen barrel, and the tail end of the adjusting bolt abuts against the rubber ring.

[0016] The pen barrel has a ball head fixed to its top end, and the diameter of the ball head is larger than the diameter of the pen barrel.

[0017] The beneficial effects of this utility model are: by adjusting the bolts and fixing the base to clamp the cell culture plate, and by using the sliding seat to slide on the top of the fixing base to provide support and guidance for the scratch pen, the scratch pen can move stably along the same straight line direction during scratching operations, which helps to ensure that the scratches are straight lines with uniform width and ensure the quality of the scratches. Attached Figure Description

[0018] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0019] Figure 1 This is a schematic diagram of the structure of the cell scratch experiment auxiliary tool of this utility model, viewed from the upper side.

[0020] Figure 2 This is a schematic diagram of the structure of the cell scratch assay auxiliary tool of this utility model, viewed from the lower side.

[0021] Figure 3 This is a schematic diagram of the fixed base in this utility model, viewed from the side and above.

[0022] Figure 4 This is a schematic diagram of the sliding seat in this utility model;

[0023] Figure 5 This is a schematic diagram of the structure of the marking pen in this utility model;

[0024] The following are the labels in the diagram: Fixed base 1, screw hole 101, strip hole 102, indicator block 103, slide groove 104, sliding seat 2, round hole 201, screw hole 202, slider 203, scratch pen 3, pen barrel 301, scratch needle 302, ball head 303, rubber ring 304, adjusting bolt 1 4, rubber protective sleeve 401, adjusting bolt 2 5. Detailed Implementation

[0025] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0026] like Figures 1 to 5 As shown, a cell scratching experiment aid tool includes a gantry-shaped fixed base 1, a sliding base 2, and a scratching pen 3.

[0027] One end of the fixed base 1 is screwed with an adjusting bolt 4 for fixing the cell culture plate. The specific installation structure is as follows: one end of the fixed base 1 has a screw hole 101 parallel to the long axis of the fixed base 1, and the adjusting bolt 4 is screwed into the screw hole 101.

[0028] The tail of the adjusting bolt 4 is fitted with a rubber protective sleeve 401 for contacting the side of the cell culture plate to prevent wear on the side of the cell culture plate when the adjusting bolt 4 is rotated.

[0029] The top of the fixed base 1 has a strip-shaped hole 102 extending along the long axis of the fixed base 1; an upward protruding indicator block 103 is provided on the fixed base 1 at both ends of the strip-shaped hole 102.

[0030] The sliding seat 2 is slidably mounted on the top of the fixed base 1, and slides in a straight line along the long axis of the fixed base. Specifically, the top of the fixed base 1 is provided with two sliding grooves 104 parallel to the strip hole 102, and the bottom end of the sliding seat 2 is fixed with two parallel sliders 203. The sliders 203 slide in cooperation with the sliding grooves 104, and the sliders 203 slide along the sliding grooves 104 to move the sliding seat 2 along the long axis of the fixed base 1.

[0031] The scratching pen 3 is installed in the center of the sliding base 2. The bottom of the scratching pen 3 is inserted into the strip hole 102 from top to bottom. When the sliding base 2 slides in a straight line along the long axis of the fixed base, the scratching pen 3 moves in the strip hole 102. Specifically, a through circular hole 201 is opened in the center of the sliding base 2, and the scratching pen 3 is installed in the circular hole 201. A screw hole 202 perpendicular to the circular hole 201 is opened on one side of the sliding base 2, and an adjusting bolt 5 for fixing the scratching pen 3 is screwed into the screw hole 202.

[0032] The scratch pen 3 includes a cylindrical pen barrel 301 and a circular rubber ring 304. A scratch needle 302 coaxial with the pen barrel 301 is fixed to the bottom end of the pen barrel 301. The rubber ring 304 is sleeved on the pen barrel 301. The tail end of the adjusting bolt 2 5 abuts against the rubber ring 304. The rubber ring 304 increases the friction between the tail end of the adjusting bolt 2 5 and the scratch pen 3 on the one hand, and prevents the adjusting bolt 2 5 from wearing down the side of the pen barrel 301 on the other hand.

[0033] A ball head 303 is fixed to the top of the pen barrel 301. The diameter of the ball head 303 is larger than the diameter of the pen barrel 301, and the ball head 303 makes it easier for the operator to hold the scratch pen 3.

[0034] The width of the strip hole 102 is greater than the diameter of the pen barrel 301 to prevent the scratch pen from sliding and rubbing against the fixed base on both sides of the strip hole 102 during movement.

[0035] The scratching needle 302 and the pen barrel 301 are made of medical stainless steel in one piece.

[0036] Work process:

[0037] First, place the fixing base 1 across the cell culture plate (six-well plate, twelve-well plate), move the fixing base so that the indicator block 103 is aligned with the position where cell scratching is required, and tighten the adjusting bolt 4 so that the adjusting bolt 4 and the fixing base 1 cooperate to clamp the cell culture plate.

[0038] The second step is to align the slider 203 with the slide groove one by one so that the sliding seat 2 can be slidably installed on the top of the fixed base. The scratch pen 3 is installed into the round hole and the bottom of the scratch pen 3 is inserted into the strip hole from top to bottom. After adjusting the installation height of the scratch pen 3, tighten the adjusting bolt two so that the tail end of the adjusting bolt two abuts against the scratch pen, thereby fixing the relative position between the scratch pen and the sliding seat.

[0039] The third step involves the operator holding the scratch pen or slide it along the square of the strip wells to make the scratching needle scratch the monolayer of cells on the cell culture plate, achieving the scratching effect required for the cell scratching experiment.

[0040] Step 4: After the scratches on a straight line are completed, remove the slide seat and scratch pen from the fixed base together. Loosen the adjusting bolt 1 to adjust the position of the fixed base across the cell culture plate. Tighten the adjusting bolt 1 again. After disinfecting the scratching needle, reinstall the slide seat and scratch pen onto the fixed base and repeat the operation process of step 3.

[0041] Fifth step: After the cell culture plate is finished being scratched, first remove the sliding seat and the scratching pen from the fixed base, then loosen the adjusting bolt to loosen the cell culture plate and remove the fixed base from the cell culture plate.

[0042] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model.

Claims

1. A tool for assisting in cell scratch assays, characterized in that, include: A fixed base, which is gantry-shaped, is screwed to one end of the fixed base with an adjusting bolt for fixing the cell culture plate, and the top of the fixed base has a strip-shaped hole extending along the long axis of the fixed base. A sliding seat is slidably mounted on the top of a fixed base, and the sliding seat slides in a straight line along the long axis of the fixed base; A scratching pen, which is mounted at the center of a sliding base, with its bottom inserted into a strip-shaped hole from top to bottom.

2. The cell scratch assay tool according to claim 1, characterized in that: An upward-protruding indicator block is provided on the fixed base at both ends of the strip hole.

3. The cell scratch assay tool according to claim 1, characterized in that: One end of the fixed base has a screw hole parallel to the long axis of the fixed base, and the adjusting bolt is screwed into the screw hole.

4. The cell scratch assay tool according to claim 1, characterized in that: The tail of the adjusting bolt is fitted with a rubber protective sleeve for abutting against the side of the cell culture plate.

5. The cell scratch assay tool according to claim 1, characterized in that: The top of the fixed base is provided with two sliding grooves parallel to the strip-shaped hole, and the bottom of the sliding seat is fixed with two parallel sliders, which slide in a one-to-one correspondence with the sliding grooves.

6. The cell scratch assay tool according to claim 1, characterized in that: The center of the sliding seat has a through-hole, and the scratch pen is installed in the hole. A screw hole two perpendicular to the hole is provided on one side of the sliding seat, and an adjusting bolt for fixing the scratch pen is screwed into the screw hole two.

7. The cell scratch assay auxiliary tool according to claim 6, characterized in that: The scratching pen includes a cylindrical pen barrel and a circular rubber ring. A scratching needle coaxial with the pen barrel is fixed to the bottom end of the pen barrel. The rubber ring is sleeved on the pen barrel, and the tail end of the adjusting bolt abuts against the rubber ring.

8. The cell scratch assay auxiliary tool according to claim 7, characterized in that: A ball head is fixed to the top of the pen barrel, and the diameter of the ball head is larger than the diameter of the pen barrel.

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