Fingertip blood detection device containing SFTSV antigen rapid detection colloidal gold test strip

Abstract

The utility model discloses a fingertip blood detection device containing an SFTSV antigen rapid detection colloidal gold test strip, and aims to simplify the operation process and improve the detection efficiency and reliability. The device comprises a deformable first cavity and a deformable second cavity which are respectively provided with a blood collection channel, a first accommodating space and a second accommodating space for packaging lysate; the first cavity and the mixed flow guide channel are communicated through a mixed flow guide channel, the first cavity and the mixed flow guide channel are blocked by a first diaphragm and a second diaphragm respectively, and the rupture strength of the first diaphragm is higher than that of the second diaphragm. During use, the first cavity is pressed, so that the diaphragms are sequentially broken, the lysis solution is sucked into the first accommodating space, then fingertip blood is sucked into the first accommodating space through the collection channel to be mixed, and finally the mixed solution is dropped into the detection test strip; the device adopts a linearly arranged cavity and a channel, so that sufficient mixing is ensured. The device integrates the functions of collecting, mixing and transferring, is suitable for rapid detection of grassroots units, and remarkably reduces the operation time and pollution risks.

Description

Technical Field

[0001] This utility model relates to the field of biomedical technology, and in particular to a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen. Background Technology

[0002] Severe fever with thrombocytopenia syndrome virus (SFTSV), recently renamed Dabiebanda virus by the International Committee on Taxonomy of Viruses (ICTV), also known as neo-Bunyavirus, is an emerging tick-borne pathogen. Infection with this virus can cause fever with thrombocytopenia syndrome (SFTS).

[0003] A current Chinese patent discloses a rapid detection colloidal gold test strip for SFTSV antigen and its preparation method. It employs a double-antibody sandwich method to detect SFTSV NP antigen, using laboratory-prepared human Dabiebanda virus N protein monoclonal antibody 1G as the detection antibody and colloidal gold-labeled human Dabiebanda virus N protein monoclonal antibody 1E as the labeling antibody. The test strip consists of a sample pad, a colloidal gold pad, a cellulose acetate membrane (NC membrane), and absorbent paper sequentially adhered to a PVC base plate along the chromatography direction. The NC membrane is coated with a detection line and a control line along the chromatography direction. The prepared rapid detection colloidal gold test strip for SFTSV antigen can qualitatively detect Dabiebanda virus in peripheral blood, serum, and plasma samples, establishing a rapid, reliable virus detection technology suitable for grassroots units and field applications.

[0004] While the above-mentioned technical solutions improve the detection efficiency and reliability of Dabie Bandar virus in blood, the blood used for testing needs to be treated with lysis buffer. The traditional method is to process the collected blood first, and then transfer the processed blood to the colloidal gold test strip for rapid detection of SFTSV antigen through an aspiration device. The process is lengthy and prone to sample contamination or waste due to improper operation.

[0005] Therefore, in view of the shortcomings of the existing technology, it is necessary to design a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen to solve the above problems.

[0006] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solution of this utility model and facilitating the understanding of those skilled in the art. It should not be assumed that the above content is known to those skilled in the art simply because it has been described in the background section of this utility model. Utility Model Content

[0007] To overcome the shortcomings of the prior art, the present invention aims to disclose a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen, so as to simplify the operation process and improve the detection efficiency and reliability.

[0008] This utility model discloses a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen, comprising:

[0009] The deformable first cavity is provided with a blood collection channel and a first accommodating space for receiving blood from the fingertip being tested. The blood collection channel is sealed by a first diaphragm.

[0010] The deformable second cavity is provided with a second accommodating space, and the second accommodating space is encapsulated with lysis solution;

[0011] The mixing guide channel is connected to the first cavity and the second cavity at its two ends, respectively, and the mixing guide channel is sealed by the second diaphragm;

[0012] The first and second diaphragms are designed to rupture under pressure, with the first diaphragm exhibiting greater rupture strength than the second. During the pressing of the first cavity, the first diaphragm ruptures first, connecting the first and second accommodating spaces. Then, as the pressure increases, the second diaphragm ruptures, connecting the first accommodating space to the outside. The blood collection channel is then connected to a fingertip blood sample. As the first cavity recovers, the fingertip blood is drawn into the first accommodating space, while simultaneously, the lysate from the second accommodating space is drawn into the first accommodating space for mixing. Finally, the first cavity is pressed again, and the mixed fingertip blood is dripped onto the SFTSV antigen rapid detection colloidal gold test strip.

[0013] The preferred technical solution is that the blood collection channel, the first accommodating space, the mixing and guiding channel, and the second accommodating space are arranged linearly in sequence to facilitate the mixing and flow of blood and lysate.

[0014] The preferred technical solution is that the volume of the first accommodating space is 1.2-2 times that of the second accommodating space, ensuring that the lysis solution can be completely drawn into the first accommodating space.

[0015] Preferred technical solution: The second chamber adopts an elastic expansion structure, which can increase the volume by 10%-30% when under pressure, facilitating the full release of the pyrolysis liquid.

[0016] Preferred technical solution: The first diaphragm and the second diaphragm are silicone films with pre-set fracture lines to ensure that they rupture as needed under pressure.

[0017] Preferred technical solution: The rupture strength of the first diaphragm is 20%-50% higher than that of the second diaphragm.

[0018] Preferred technical solution: The blood collection channel is also equipped with an anti-backflow valve to prevent blood from flowing back.

[0019] Preferred technical solution: The anti-backflow valve is a duckbill-type one-way valve, which has a simple structure and good sealing performance.

[0020] Due to the application of the above technical solution, the beneficial effects of this utility model compared with the prior art are as follows:

[0021] 1) It integrates blood collection, lysis buffer mixing and sample transport functions into one unit, requiring no additional tools or complicated steps, significantly reducing operation time; blood and lysis buffer mixing and transport can be completed by pressing the cavity, making it suitable for grassroots units and rapid on-site testing.

[0022] 2) The built-in lysis buffer and closed design avoid the sample exposure process in traditional methods, reducing the possibility of contamination; the anti-backflow valve further prevents blood backflow or external contamination.

[0023] 3) The linear arrangement of channels and cavities facilitates liquid flow and mixing.

[0024] 4) Optimized volume ratio and diaphragm design ensure thorough mixing of blood and lysis buffer, improving detection accuracy. Attached Figure Description

[0025] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the structure of a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to the present invention.

[0027] In the above figures, 1 is the first cavity; 11 is the blood collection channel; 12 is the first accommodating space; 13 is the first diaphragm; 14 is the anti-backflow valve; 2 is the second cavity; 21 is the second accommodating space; 22 is the lysate; 3 is the mixing guide channel; and 31 is the second diaphragm. Detailed Implementation

[0028] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0029] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate for the description of embodiments of this application herein. Furthermore, the terms "comprising" and "having," and their synonyms, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0030] In this application, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing the present invention and its embodiments, and are not intended to limit the indicated device, element, or component to having a specific orientation, or to be constructed and operated in a specific orientation.

[0031] Furthermore, in addition to indicating direction or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in some cases to indicate a certain dependency or connection relationship. Those skilled in the art can understand the specific meaning of these terms in this utility model according to the specific circumstances.

[0032] Furthermore, the terms "installation," "setting," "equipped with," "connection," "linking," "fitting," and "fitting" should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Similarly, "fitting" can mean completely or partially fitted. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0033] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0034] Example:

[0035] like Figure 1As shown, this utility model discloses a finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen, including a first cavity 1, a second cavity 2, and a mixing and guiding channel 3. The main components of this utility model will be described in detail below:

[0036] like Figure 1 As shown, the deformable first cavity 1 is provided with a blood collection channel 11 for collecting fingertip blood and a first accommodating space 12 for receiving the fingertip blood to be tested. The blood collection channel 11 is sealed by a first diaphragm 13.

[0037] like Figure 1 As shown, the deformable second cavity 2 has a second accommodating space 21, and the second accommodating space 21 is encapsulated with lysis solution 22.

[0038] like Figure 1 As shown, the mixing guide channel 3 is connected to the first cavity 1 and the second cavity 2 at both ends, and the mixing guide channel 3 is sealed by the second diaphragm 31.

[0039] like Figure 1 As shown, the first diaphragm 13 and the second diaphragm 31 can rupture under pressure, and the rupture strength of the first diaphragm 13 is higher than that of the second diaphragm 31.

[0040] The method of use and principle of this utility model are as follows:

[0041] Initial state: The first diaphragm 13 and the second diaphragm 31 respectively block the mixing channel 3 and the blood collection channel 11.

[0042] Usage instructions:

[0043] Step 1: Press the first cavity 1 for the first time. The pressure is transmitted to the first diaphragm 13 and the second diaphragm 31. Because the rupture strength of the first diaphragm 13 is higher than that of the second diaphragm 31, the second diaphragm 31 ruptures first, and the first accommodating space 12 and the second accommodating space 21 are connected.

[0044] Step two, continue pressing, the first diaphragm 13 ruptures, and the first accommodating space 12 is connected to the outside through the blood collection channel 11.

[0045] Step 3: Align the blood collection channel 11 with the fingertip blood and release the pressure. The first chamber 1 deforms and recovers to generate negative pressure. The fingertip blood is drawn into the first accommodating space 12 through the channel 11. At the same time, the lysis solution 22 enters the first accommodating space 12 through the mixing guide channel 3 and mixes thoroughly with the fingertip blood.

[0046] Step 4: Press the first chamber 1 again. The mixture will drip through channel 11 onto the rapid detection colloidal gold test strip to complete the test.

[0047] like Figure 1As shown, the blood collection channel 11, the first accommodating space 12, the mixing and guiding channel 3, and the second accommodating space 21 are arranged linearly in sequence, which facilitates liquid flow and mixing during use.

[0048] like Figure 1 As shown, the volume of the first accommodating space 12 is 1.2-2 times that of the second accommodating space 21, ensuring that the lysis solution 22 is completely drawn into the first accommodating space 12.

[0049] like Figure 1 As shown, the second cavity 2 adopts an elastic expansion structure, which can increase the volume by 10%-30% when under pressure. When the second cavity 2 expands and then rebounds, it can promote the pyrolysis liquid 22 inside to enter the first accommodating space 12.

[0050] like Figure 1 As shown, the first diaphragm 13 and the second diaphragm 31 are silicone films with pre-set fracture lines to ensure that they break as needed.

[0051] like Figure 1 As shown, the burst strength of the first diaphragm 13 is 20%-50% higher than that of the second diaphragm 31.

[0052] like Figure 1 As shown, the blood collection channel 11 is also equipped with an anti-backflow valve 14 to prevent blood from flowing back from the fingertip.

[0053] like Figure 1 As shown, the anti-backflow valve 14 is a duckbill type check valve.

[0054] This invention achieves integrated operation of blood collection, lysis buffer mixing, and sample transport through an integrated design. The closed-loop press-driven structure significantly simplifies the process, reduces the risk of contamination, and ensures the high efficiency and accuracy of rapid testing.

[0055] Finally, it should be noted that the above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen, characterized in that, include: The deformable first cavity (1) is provided with a blood collection channel (11) and a first accommodating space (12) for receiving blood from the fingertip being tested. The blood collection channel (11) is sealed by a first diaphragm (13). The deformable second cavity (2) is provided with a second accommodating space (21), and the second accommodating space (21) is encapsulated with pyrolysis solution; The mixing guide channel (3) is connected to the first cavity (1) and the second cavity (2) at both ends, and the mixing guide channel (3) is blocked by the second diaphragm (31); The first diaphragm (13) and the second diaphragm (31) can rupture under pressure, and the rupture strength of the first diaphragm (13) is higher than that of the second diaphragm (31).

2. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 1, characterized in that: The blood collection channel (11), the first accommodating space (12), the mixing and guiding channel (3), and the second accommodating space (21) are arranged linearly in sequence.

3. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 1, characterized in that: The volume of the first accommodating space (12) is 1.2-2 times that of the second accommodating space (21).

4. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 1, characterized in that: The second cavity (2) adopts an elastic expansion structure, and its volume can increase by 10%-30% when under pressure.

5. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 1, characterized in that: The first diaphragm (13) and the second diaphragm (31) are silicone films with pre-set fracture lines.

6. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 5, characterized in that: The rupture strength of the first diaphragm (13) is 20%-50% higher than that of the second diaphragm (31).

7. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 1, characterized in that: The blood collection channel (11) is also equipped with an anti-backflow valve (14).

8. The finger-prick blood testing device containing a colloidal gold test strip for rapid detection of SFTSV antigen according to claim 7, characterized in that: The anti-backflow valve (14) is a duckbill-type one-way valve.

Images