Hanging accessory for separation process of hematopoietic stem cells of umbilical cord blood

Abstract

The utility model relates to the technical field of biological experiment instruments, in particular to a hanging accessory used in the separation process of umbilical cord blood hematopoietic stem cells, which is mainly used for being connected with a grid at the top of a biological safety cabinet and comprises a fixed end connected with the grid at the top of the biological safety cabinet, and a hook used for being connected with a blood bag is arranged below the fixed end. The fixing end comprises a clamping ring, a torsional spring clamping opening capable of being clamped into a grid is formed in the clamping ring, a limiting sliding groove is formed in the inner side of the clamping ring, and a limiting rod capable of moving up and down along the inner wall of the clamping ring is slidably connected into the limiting sliding groove. A reset spring capable of resetting the limiting rod upwards is arranged between the bottom of the limiting sliding groove and the limiting rod. The technical problems that in the prior art, a blood bag is connected with a grid in a biological safety cabinet through a paper clip, then the blood bag is hung, shaking is large, the blood bag is prone to falling off, a sample possibly needs to be centrifuged many times, and the motility rate of the sample is affected are solved.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, specifically a suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells. Background Technology

[0002] Umbilical cord blood hematopoietic stem cell separation technology refers to the process of separating hematopoietic stem cells from umbilical cord blood, which contains various components such as red blood cells, plasma, and white blood cells. The core of the separation is to remove red blood cells and plasma, retain the white membrane layer (nucleated cell layer) rich in hematopoietic stem cells, and concentrate the hematopoietic stem cells. To prevent sample contamination and ensure cell viability, this operation is usually performed in a biosafety cabinet.

[0003] A biological safety cabin (BSC) is a box-shaped workbench designed to prevent the aerosol release of hazardous or unknown biological particles during experimental procedures. It resembles a four-legged table with a box-shaped cover at the top. The upper chamber contains a negative pressure air purification system that creates negative pressure, causing air inside the cabin to flow upwards and outside air to flow inwards. This prevents air from escaping and thus avoids the release of unknown gases. To facilitate better airflow to the negative pressure air purification system, the bottom of the cabin features crisscrossing horizontal and vertical beams forming a permeable mesh.

[0004] After centrifuging umbilical cord blood, the blood bag needs to be suspended to slowly separate the upper plasma layer from the lower hematopoietic stem cells. Typically, lab personnel would install a hanging rack in the biosafety cabinet to suspend the blood bag. However, this reduces the operating space within the cabinet, making operation inconvenient. Therefore, lab personnel often hang the blood bag on the top grid of the biosafety cabinet, keeping it suspended. This method eliminates the need for additional hanging racks, reduces the number of items on the biosafety cabinet surface, and facilitates operation. Therefore, it is widely adopted by lab personnel.

[0005] To connect the blood bag to the grid at the top of the biosafety cabinet, the operator uses a paperclip to attach to the connection hole at the top of the blood bag and then hangs the paperclip on the grid at the top of the biosafety cabinet, thus suspending the blood bag inside. However, directly suspending the blood bag using a paperclip leaves a gap between the paperclip and the top grid, making it prone to swaying on the grid. This can cause the centrifuged plasma to remix, and there is also a risk that the blood bag may fall during use, potentially leading to multiple centrifugations and affecting sample viability. Utility Model Content

[0006] The purpose of this invention is to provide a suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells, in order to solve the technical problems mentioned above in the prior art, which uses paperclips to connect blood bags to the mesh inside the biosafety cabinet and then suspends the blood bags, resulting in large shaking, easy falling of the blood bags, and the need for multiple centrifugations of the samples, which affects the sample viability.

[0007] To solve the above problems, the technical solution adopted by this utility model is as follows: a suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells, including a fixed end connected to the top grid of a biosafety cabinet, a hook for connecting to a blood bag below the fixed end, the fixed end including a snap ring, the snap ring having a torsion spring snap interface that can snap into the grid, a limiting groove opening on the inner side of the snap ring, a limiting rod that can move up and down along the inner wall of the snap ring slidably connected in the limiting groove, and a return spring that can reset the limiting rod upward between the bottom of the limiting groove and the limiting rod.

[0008] The beneficial effects of this implementation plan are as follows:

[0009] 1. In existing technologies, after centrifuging umbilical cord blood, the blood bag needs to be suspended to allow gravity to slowly separate the upper plasma layer from the lower hematopoietic stem cells. Typically, operators suspend the blood bag on the top grid of a biosafety cabinet. To connect the blood bag to the grid, operators use a paperclip to attach to the connection hole on the top of the blood bag and then hang it on the grid. While this method eliminates the need for additional hanging supports, reducing the number of items on the biosafety cabinet surface and simplifying operation, the gap between the paperclip and the top grid allows the blood bag to wobble, causing the centrifuged plasma to remix. Furthermore, the wobbling poses a risk of the blood bag falling during use, potentially requiring multiple centrifugations, affecting sample viability and ultimately impacting experimental results. This application features a snap-fit ​​ring, which uses a torsion spring-loaded interface to snap onto the crossbeam of the grid, easily connecting to the top grid of the biosafety cabinet. Once snapped in, the limiting rod inside the snap-fit ​​ring, under the action of the return spring, firmly abuts against the lower end of the top grid crossbeam of the biosafety cabinet, preventing it from wobbling. Therefore, compared with existing technologies, this application provides a more stable connection to the grid and is less prone to wobbling.

[0010] Furthermore, the limiting groove is a convex-shaped structure with an opening size larger than the internal size, and the limiting rod has convex-shaped sliders at both ends, the convex-shaped sliders being the same size as the limiting groove.

[0011] Furthermore, the limiting rod is provided with a limiting recess that can accommodate the grid beam.

[0012] Furthermore, the bottom of the hook groove is a V-shaped groove.

[0013] Furthermore, the hook also includes a closing rod that can close the hook opening.

[0014] Furthermore, the hook head is provided with a magnetic suction head, and the closed rod is provided with a hook cap made of magnetic material, the inner diameter of the hook cap being the same as the outer diameter of the magnetic suction head. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model. Detailed Implementation

[0016] The following detailed description illustrates the specific implementation method:

[0017] The reference numerals in the accompanying drawings include: snap ring 1, torsion spring snap interface 11, snap mounting plate 111, snap head 112, rotating shaft 113, limiting rod 12, limiting groove 121, limiting slide groove 122, hook 2, hook post 21, V-shaped hook bottom 211, magnetic head 212, closing rod 22, sliding sleeve 221, and hook cap 222.

[0018] Implementation, for example, attached Figure 1 As shown:

[0019] A suspension accessory for the separation of umbilical cord blood hematopoietic stem cells, primarily used for connection to the top mesh of a biosafety cabinet. The fixed end includes a snap-fit ​​ring 1, which is annular in structure, but not perfectly circular; rather, it has semicircular ends and a rectangular middle section. Figure 1 The direction is described as follows: a torsion spring locking interface 11 is provided at the connection between the upper semicircular structure and the rectangular straight section at the left end of the locking ring 1. The torsion spring locking interface 11 includes a locking head 112. The end of the portion extending downward from the upper semicircular structure at the left end of the locking ring 1 forms a locking mounting plate 111. A connecting thin plate is provided at the upper end of the locking head 112. The thickness of the connecting thin plate of the locking mounting plate 111 and the locking head 112 is half the thickness of the locking ring 1. When the two are put together, their thickness is the same as that of the locking ring 1. A rotating shaft 113 mounting hole is opened at the connection head of the locking mounting plate 111 and the locking head 112. Therefore, by passing the rotating shaft 113 through the mounting holes of both, the locking head 112 and the locking mounting plate 111 can be hinged. A torsion spring is sleeved on the rotating shaft 113. The two ends of the torsion spring are fixedly connected to the locking head 112. Therefore, when the locking head 112 is pressed... The latch head 112 will rotate, but it will automatically reset after being released.

[0020] by Figure 1The direction is described in terms of orientation. The inner wall of the rectangular structure of the snap ring 1 has a limiting groove 122 in the vertical direction. The limiting groove 122 is provided on both opposite sides of the snap ring 1, and its properties and dimensions are completely identical. The limiting groove 122 is a U-shaped groove, with its opening size smaller than its internal size. A limiting rod 12, which can move up and down along the inner wall of the snap ring, is slidably connected within the limiting groove 122. The limiting rod 12 has U-shaped sliders at both ends, whose dimensions are identical to the limiting groove 122, allowing it to be snapped into the limiting groove 122 to form a sliding connection. A return spring is provided between the bottom of the lowest end of the limiting groove 121 and the U-shaped slider of the limiting rod 12. Therefore, when the return spring does not deform, it will lift the limiting rod 12 upwards. It is worth noting that the limiting groove 122 in this application is also provided on the inner side of the latch head 112. When no external force is applied, the return spring can lift the limiting rod 12 to the connection position between the semi-circular structure and the rectangular structure at the upper end of the latching ring 1. Therefore, in use, you need to hold the limiting rod 12 with your finger and slide it downwards to align the torsion spring latch interface 11 on the latching ring 1 with the grid at the upper end of the biosafety cabinet and press it down, so that the latch head 112 rotates and the latching ring 1 is engaged in the grid. At this time, release the finger holding the limiting rod 12, the return spring returns to its deformation, the limiting rod 12 slides upwards, and presses against the lower end face of the grid beam to complete the limiting. In order to make the limiting rod 12 better press against the lower end of the grid beam, a limiting groove 121 is provided in the middle of the limiting rod 12. It is recessed downwards to accommodate the grid beam and to prevent the grid beam from shaking by using the two side walls of the recess.

[0021] A hook 2 is provided below the fixed end. The hook 2 is welded to the lower end of the snap ring 1. The hook 2 first uses a vertically downward cylindrical structure, then sets a semi-circular bend to form a hook structure. A hook post 21 is set at the upper end of the hook structure to form a deep groove, thus ensuring that the blood bag hanging on the hook 2 will not easily slip off the hook. In order to make the blood bag hang on the hook 2 stably, a V-shaped hook bottom 211 is made on the inner side of the lowest point of the semi-circular bend of the hook 2. The V-shaped structure locks the connection hole of the blood bag, making it less likely to shake. A closing rod 22 is also provided on the hook 2. One end of the closing rod 22 is a sliding sleeve 211 that fits onto the hook body. The sliding sleeve 211 and the hook 2 form a clearance fit, allowing the sliding sleeve to slide up and down along the body of the hook 2. The other end of the closing rod 22 is a hook cap 222, which is a cap that just covers the hook post 21. A magnetic head 212 is also provided at the upper end of the hook post 21. The hook cap 222 is also made of magnetic material. Therefore, when the hook cap 222 is used to close the hook 2, it will have a certain magnetic attraction to ensure that the hook cap 222 does not jump off.

[0022] The above descriptions are merely embodiments of this utility model, and common knowledge regarding specific structures and characteristics is not elaborated upon here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this utility model, and these should also be considered within the scope of protection of this utility model. These modifications will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A suspension accessory for the separation of umbilical cord blood hematopoietic stem cells, comprising a fixed end connected to the top mesh of a biosafety cabinet, and a hook below the fixed end for connecting to a blood bag, characterized in that: The fixed end includes a snap ring, which has a torsion spring snap interface that can snap into the mesh. A limit groove is opened on the inner side of the snap ring, and a limit rod that can move up and down along the inner wall of the snap ring is slidably connected in the limit groove. A return spring that can reset the limit rod upward is provided between the bottom of the limit groove and the limit rod.

2. The suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells according to claim 1, characterized in that: The limiting groove is a convex-shaped structure with an opening size larger than the internal size. The limiting rod has convex-shaped sliders at both ends, and the convex-shaped sliders have the same size as the limiting groove.

3. The suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells according to claim 2, characterized in that: The limiting rod is provided with a limiting recess that can accommodate the grid beam.

4. The suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells according to claim 2, characterized in that: The bottom of the hook trench is V-shaped.

5. The suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells according to claim 2, characterized in that: The hook also includes a closing rod that can close the hook opening.

6. The suspension accessory for the separation process of umbilical cord blood hematopoietic stem cells according to claim 5, characterized in that: The hook has a magnetic head, and the closed rod has a magnetic cap. The inner diameter of the cap is the same as the outer diameter of the magnetic head.

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