Reaction liquid carrier

Abstract

The utility model provides a reaction liquid carrier which comprises a first cavity structure and a second cavity structure, the heat transfer structure is formed on the outer side and / or the inner side of the reaction cavity, and the heat transfer structure is connected with the first cavity structure. The utility model solves the problem that the heat transfer efficiency and the detection efficiency of the PCR reaction liquid carrier device for QPCR are low.

Description

Technical Field

[0001] This utility model relates to the field of DNA measurement equipment technology, and in particular to a reaction liquid carrier. Background Technology

[0002] The description in this section provides only background information related to the disclosure of this utility model and does not constitute prior art.

[0003] PCR (Polymerase Chain Reaction) is a molecular biology experimental method for the in vitro enzymatic synthesis of specific DNA fragments. The basic principle of PCR is similar to DNA replication in vivo, and its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation, annealing, and extension. Denaturation of the template DNA involves heating it at high temperature for a certain time to dissociate it into single strands, allowing it to bind to the primers. Annealing requires cooling the template DNA to a specific temperature to facilitate primer-template binding according to the complementary base pairing principle. Extension involves raising the temperature to the optimal reaction temperature of DNA polymerase, allowing the primers to form a semi-conservative replicating strand complementary to the template DNA strand according to the base pairing and semi-conservative replication principle. Through several cycles of these three steps (denaturation, annealing, and extension), the target gene can be amplified millions of times. Therefore, temperature control of the PCR reaction solution significantly affects the replication efficiency and effect of PCR.

[0004] Furthermore, in qPCR (Quantitative Real-time Polymerase Chain Reaction), where fluorescent dyes or fluorescent groups are added to the PCR amplification reaction system, the amount of amplified product in each cycle is monitored in real time by collecting fluorescence signals throughout the PCR process. Finally, the sample is quantitatively analyzed using a standard curve and CT values. It is evident that qPCR, compared to traditional PCR, requires multiple photometric measurements to plot data at different reaction stages. Therefore, for qPCR, the heating and cooling rates significantly impact its measurement efficiency and reaction performance. In a PCR instrument, a reaction chamber is typically provided to hold the reaction solution, and a heating device is used to heat the chamber. During annealing, heating is stopped, and a cooling device is used to rapidly cool the reaction solution within the chamber. When photometric measurement is required, the reaction solution is removed. The entire qPCR measurement process involves numerous steps and is inefficient, severely impacting the replication efficiency and effectiveness. Therefore, some PCR instruments offer qPCR modules to improve qPCR measurement efficiency. These modules may incorporate features such as zoned temperature control channels, multi-chamber heating structures, and multiple fluorescence detection channels. Zoned temperature control channels implement different temperature zones for different flow areas to enhance replication efficiency, but this significantly impacts PCR reaction quality. Multi-chamber heating structures require high-precision pipettes to ensure uniform liquid volume in each chamber, preventing temperature variations after heating. Furthermore, to facilitate liquid addition and removal, heating is limited to the bottom of each chamber, severely affecting the temperature rise and fall rates of the liquid within each chamber. The use of multiple fluorescence detection channels necessitates that the diameter of the photometric spot be smaller than that of the fluorescence detection channel to allow the spot to pass through and irradiate the reaction chamber. This limits the minimum diameter of the reaction chamber to meet the photometric requirements of the instrument. The diameter of the reaction chamber also affects the heating and cooling rates of the liquid within each chamber, further contributing to the low qPCR replication efficiency. Furthermore, the heating device is located outside each chamber, heating it via thermal radiation. The significant energy loss during heat transfer due to this radiation also affects the heating and cooling rates of the liquid within each chamber, resulting in lower qPCR replication efficiency.

[0005] It should be noted that the above introduction to the technical background is only for the purpose of providing a clear and complete explanation of the technical solutions of this utility model and facilitating understanding by those skilled in the art. It should not be assumed that these technical solutions are known to those skilled in the art simply because they have been described in the background section of this utility model. Utility Model Content

[0006] The purpose of this invention is to provide a reaction liquid carrier that solves the problem of low heat transfer efficiency and detection efficiency of PCR reaction liquid carrier devices used in qPCR.

[0007] The above-mentioned objectives of this utility model are mainly achieved by the following technical solutions:

[0008] This utility model provides a reaction liquid carrier, comprising:

[0009] The first cavity structure has a reaction chamber formed inside it;

[0010] A heat transfer structure is formed on the outside and / or inside the reaction chamber, and the heat transfer structure is connected to the first chamber structure.

[0011] In one specific embodiment, the heat transfer structure includes at least one heat transfer plate, which extends outward from the outside of the reaction chamber.

[0012] In one specific embodiment, there are two heat transfer plates, which are disposed on opposite sides of the first cavity structure.

[0013] In one specific embodiment, the heat transfer structure includes at least one intermediate plate, which is disposed inside the reaction chamber and divides the reaction chamber into at least two temperature control chambers.

[0014] In one specific embodiment, multiple temperature control chambers are isolated from each other. The first chamber structure has multiple liquid inlets and multiple liquid outlets, and the multiple liquid inlets and multiple liquid outlets are respectively connected to each of the temperature control chambers.

[0015] In one specific embodiment, a plurality of temperature control cavities are connected sequentially along their arrangement direction, and the first cavity structure has at least one liquid inlet and at least one liquid outlet, wherein at least one liquid inlet and at least one liquid outlet are respectively connected to the plurality of temperature control cavities.

[0016] In one specific embodiment, the intermediate plate and the inner wall of the first cavity structure are divided to form a liquid passage, and two adjacent temperature control cavities are connected through the liquid passage.

[0017] In one specific embodiment, the intermediate plate has a liquid passage, which is connected to two adjacent temperature control cavities.

[0018] In one specific embodiment, multiple temperature control chambers are connected end-to-end through the liquid passage.

[0019] In one specific embodiment, multiple temperature control cavities are stacked, the liquid inlet is connected to the temperature control cavity located at the top of the reaction liquid carrier, the outlet is connected to the temperature control cavity located at the bottom of the reaction liquid carrier, and the first cavity structure also has an exhaust cavity, one end of the exhaust cavity is connected to the outside, and the other end of the exhaust cavity is connected to the outlet.

[0020] In one specific embodiment, a plurality of temperature control chambers are arranged at intervals along the same plane, the liquid inlet is connected to the temperature control chamber located at one end of the plurality of temperature control chambers, and the outlet is connected to the temperature control chamber located at the other end of the plurality of temperature control chambers.

[0021] In one specific embodiment, a plurality of temperature control chambers are arranged at intervals along the same plane, and there are a plurality of outlets, each of which is connected to one end of a plurality of temperature control chambers.

[0022] In one specific embodiment, the outlet is provided with a one-way structure for gas passage only.

[0023] In one specific embodiment, the first cavity structure has a light-transmitting inner wall that exposes at least one of the temperature-controlled cavities, and the first cavity structure also has an incident area, the two ends of which correspond to the light-transmitting inner wall and the external arrangement of the first cavity structure, respectively.

[0024] In one specific embodiment, the first cavity structure further includes a photometering area, the two ends of which correspond to the light-transmitting inner wall and the external arrangement of the first cavity structure, respectively.

[0025] In one specific embodiment, the first cavity structure has two opposing light-transmitting inner walls, and the photometric area and the incident area are respectively arranged corresponding to the two light-transmitting inner walls.

[0026] In one specific embodiment, the intermediate plate has a light-transmitting structure facing the two adjacent reaction chambers.

[0027] In one specific embodiment, there are multiple intermediate plates, and the light-transmitting structures of the multiple intermediate plates are spaced apart along the opening direction of the incident area.

[0028] In one specific embodiment, the surface of the intermediate plate is arranged perpendicular to the opening direction of the incident area.

[0029] In one specific embodiment, the first cavity structure further includes a support member connected between two adjacent intermediate plates, and the support member and the two adjacent intermediate plates divide to form the temperature control cavity.

[0030] In one specific embodiment, the light-transmitting inner wall is formed on the side wall of the support member, and the photometric area and the incident area are disposed facing at least one of the light-transmitting inner walls of the support member.

[0031] In one specific embodiment, the first cavity structure has an upper plate and a lower plate stacked together, the upper plate and the lower plate being pressed together to form the reaction cavity, and the heat transfer plate located outside the reaction cavity.

[0032] In one specific embodiment, the intermediate plate is connected between the upper plate and the lower plate, and the intermediate plate, the upper plate and the lower plate are pressed together to form the reaction chamber. The intermediate plate is located inside the reaction chamber, and the intermediate plate, the upper plate and the lower plate are all made of thermally conductive material.

[0033] In one specific embodiment, the thermal conductivity of the intermediate plate, the upper plate, and the lower plate is preferably not less than 200 W / m·K.

[0034] In one specific embodiment, the first cavity structure has an upper membrane, a middle plate, and a lower membrane stacked together. The upper membrane, the middle plate, and the lower membrane are pressed together to form the reaction cavity and the intermediate plate located inside the reaction cavity. The middle plate is made of a thermally conductive material, and the upper membrane and the lower membrane are made of a light-transmitting thermally insulating material that does not interfere with the detection results of the reaction liquid in the reaction cavity.

[0035] In one specific embodiment, the thermal conductivity of the middle layer is preferably not less than 200 W / m·K, and the thermal conductivity of the upper layer and the lower layer is preferably not more than 0.2 W / m·K.

[0036] In one specific embodiment, the thickness of the upper plate and the lower plate is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0037] In one specific embodiment, the thickness of the heat transfer plate is greater than or equal to 0.3 mm and less than or equal to 2 mm.

[0038] In one specific embodiment, the thickness of the intermediate plate is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0039] In one specific embodiment, the width of the temperature control cavity is set to be on the same order of magnitude as the width of the intermediate plate.

[0040] In one specific embodiment, the width of the temperature control cavity is greater than or equal to 0.1 mm and less than or equal to 0.5 mm compared to the width of the intermediate plate.

[0041] In one specific embodiment, the thickness of the middle layer plate is greater than or equal to 0.1 mm and less than or equal to 1 mm.

[0042] In one specific embodiment, the height of the temperature control cavity is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0043] In one specific embodiment, the thickness of the support member is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0044] In one specific embodiment, the upper plate and the lower plate have thermally conductive layers. The lower surface of the thermally conductive layer of the upper plate is provided with an isolation layer, and the upper surface of the thermally conductive layer of the lower plate is provided with the isolation layer. The isolation layer is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

[0045] In one specific embodiment, the thermally conductive layer is preferably made of aluminum, and the insulating layer is preferably made of a plastic film or an aluminum oxide film.

[0046] In one specific embodiment, the intermediate plate has a heat-conducting layer, and both the upper and lower surfaces of the heat-conducting layer of the intermediate plate are provided with an isolation layer, the isolation layer being made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

[0047] In one specific embodiment, the thermally conductive layer is preferably made of aluminum, and the insulating layer is preferably made of a plastic film or an aluminum oxide film.

[0048] In one specific embodiment, an isolation layer is formed on the surface of the middle layer plate, and the thickness of the isolation layer, the upper film, and the lower film is less than or equal to 50 μm. The isolation layer is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

[0049] In one specific embodiment, the middle layer is made of aluminum, the insulating layer is made of alumina film, and the upper and lower films are formed of carbonate or polypropylene materials.

[0050] In one specific embodiment, the reaction liquid carrier further includes:

[0051] A flow guiding structure has a flow guiding channel and an exhaust channel that pass through it. The flow guiding channel is connected to the liquid inlet, and the exhaust channel is connected to the outlet through the exhaust chamber.

[0052] In one specific embodiment, there are multiple first cavity structures, which are stacked on top of each other, and the temperature control cavities of each first cavity structure overlap along their stacking direction.

[0053] This utility model also provides a reaction liquid carrier, comprising: a first cavity structure having a reaction cavity formed therein; a heat transfer structure formed on the outer and inner sides of the reaction cavity, the heat transfer structure being connected to the first cavity structure, the heat transfer structure including at least one intermediate plate, the at least one intermediate plate being disposed on the inner side of the reaction cavity and dividing the reaction cavity into multiple temperature control cavities.

[0054] Compared with the prior art, the technical solution of this utility model has the following features and advantages:

[0055] 1. The reaction liquid carrier provided by this utility model has a heat transfer structure set on the outside of the reaction chamber, on which a heat source device or a cold source device can be attached. Through the connection between the first chamber structure and the heat transfer structure, heat energy or cold energy is transferred to the first chamber structure to change the temperature of the first chamber structure, thereby changing the temperature of the reaction liquid in the reaction chamber. A heat transfer structure is set on the inside of the reaction chamber, on which the heat energy or cold energy received by the first chamber structure can be transferred to the reaction chamber to change the temperature of the reaction liquid in the reaction chamber. The heat transfer structure set on the inside of the reaction chamber can also increase the contact area between the reaction liquid in the reaction chamber and the heat conduction structure or component, further improving the temperature control efficiency of the reaction liquid.

[0056] 2. The intermediate plate also divides the reaction chamber into multiple temperature control chambers. A smaller volume of reaction liquid can be allocated to a larger heating or cooling surface, making the temperature control efficiency of the reaction liquid higher. At the same time, dividing the reaction chamber into temperature control chambers can also reduce the problem that the temperature control efficiency of the reaction liquid is limited by the internal heat transfer rate of the liquid due to the thickness of the liquid.

[0057] 3. By designing a transparent inner wall, an incident area, and a photometric area, the irradiation device can directly irradiate the temperature control cavity from the outside of the reaction solution carrier, and the photometric device can directly acquire fluorescence signals from inside the reaction solution carrier. This avoids the need to remove the reaction solution after each reaction, which could lead to low photometric efficiency in qPCR. The use of an incident area and a photometric area exposes the reaction cavity to the outside, improving the photometric efficiency of each temperature control cavity. The transparent inner wall structure exposes the temperature control cavity to the outside, increasing the area that can receive light, thus increasing the amount of light entering each temperature control cavity within a relatively small volume.

[0058] 4. By arranging multiple temperature control chambers, the number of temperature control chambers can be increased and the volume and thickness of each temperature control chamber can be reduced without changing the overall thickness of the reaction chamber, thereby further improving the temperature control efficiency of each chamber. Furthermore, the stacked arrangement of temperature control chambers, with each chamber exposing the same side, ensures that the light emitted by the irradiation device can enter each temperature control chamber, enabling the reaction liquid in each chamber to produce a fluorescence effect and complete the photometric step. This avoids the problem of light emitted by the irradiation device creating a light slit effect or failing to enter due to the reduced thickness of the temperature control chamber, thus preventing the problem of insufficient light inlet for photometric measurement. Attached Figure Description

[0059] Figure 1 This is a structural diagram of the first embodiment of the reaction liquid carrier of this utility model;

[0060] Figure 2 This is a structural diagram of the second embodiment of the reaction liquid carrier of this utility model;

[0061] Figure 3 This is a structural diagram of the third embodiment of the reaction liquid carrier of this utility model;

[0062] Figure 4 This is a structural diagram of the fourth embodiment of the reaction liquid carrier of this utility model;

[0063] Figure 5 This is a structural diagram of the fifth embodiment of the reaction liquid carrier of this utility model;

[0064] Figure 6 This is an exploded view of the fifth embodiment of the reaction liquid carrier of this utility model;

[0065] Figure 7 This is a top view of the first embodiment of the reaction liquid carrier of this utility model;

[0066] Figure 8 This is a top view of the second embodiment of the reaction liquid carrier of this utility model;

[0067] Figure 9 This is a front view of the first embodiment of the reaction liquid carrier of this utility model;

[0068] Figure 10 This is a front view of the fourth embodiment of the reaction liquid carrier of this utility model;

[0069] Figure 11 This is a front view of the third embodiment of the reaction liquid carrier of this utility model;

[0070] Figure 12 for Figure 9 A partially enlarged view of the first embodiment's structural diagram;

[0071] Figure 13 for Figure 9 A partial enlarged view of the second embodiment's structural diagram;

[0072] Figure 14 for Figure 9 A partial enlarged view of the third embodiment's structural diagram;

[0073] Figure 15 for Figure 9 A partial enlarged view of the fourth embodiment's structural diagram;

[0074] Figure 16 for Figure 10 A partially enlarged view of the first embodiment's structural diagram;

[0075] Figure 17 for Figure 10 A partial enlarged view of the second embodiment's structural diagram;

[0076] Figure 18 for Figure 10 A partial enlarged view of the third embodiment's structural diagram;

[0077] Figure 19 for Figure 10 A partial enlarged view of the fourth embodiment's structural diagram;

[0078] Figure 20 for Figure 10 A partial enlarged view of the fifth embodiment's structural diagram;

[0079] Figure 21 for Figure 10 The sixth embodiment structural diagram is shown in a partially enlarged view;

[0080] Figure 22 for Figure 10 The seventh embodiment structural diagram is shown in a partially enlarged view;

[0081] Figure 23 for Figure 10 The enlarged view of the eighth embodiment's structural diagram;

[0082] Figure 24 for Figure 10 A partial enlarged view of the structural diagram of the ninth embodiment;

[0083] Figure 25 for Figure 10 The tenth embodiment structural diagram is shown in a partially enlarged view;

[0084] Figure 26 This is a structural diagram of a third embodiment of the reaction liquid carrier of this utility model;

[0085] Figure 27This is a structural diagram of another embodiment of the reaction liquid carrier of this utility model;

[0086] Figure 28 This is a structural diagram of yet another embodiment of the reaction liquid carrier of this utility model;

[0087] Figure 29 This is a structural diagram of the flow guiding structure of the reaction liquid carrier of this utility model;

[0088] Figure 30 This is a structural diagram of an embodiment of the first cavity structure of the reaction liquid carrier of this utility model;

[0089] Figure 31 This is a structural diagram of an embodiment of the first cavity structure and heat transfer structure of the reaction liquid carrier of this utility model;

[0090] Figure 32 This is a structural diagram of another embodiment of the first cavity structure and heat transfer structure of the reaction liquid carrier of this utility model.

[0091] Explanation of icon numbers:

[0092] 1. First cavity structure; 11. Reaction chamber; 111. Temperature control chamber; 12. Liquid inlet; 13. Outlet; 14. Transparent inner wall; 15. Incident area; 16. Measuring area; 171. Upper plate; 172. Lower plate; 173. Middle plate; 174. Upper membrane; 175. Lower membrane; 1701. Thermally conductive layer; 1702. Isolation layer;

[0093] 2. Heat transfer structure; 21. Heat transfer plate; 22. Intermediate plate;

[0094] 3. Liquid passage;

[0095] 4. Flow guiding structure; 41. Flow guiding channel; 42. Exhaust channel;

[0096] 5. Unidirectional structure;

[0097] 6. Exhaust chamber;

[0098] 7. Support components;

[0099] 8. Light-transmitting structure. Detailed Implementation

[0100] To enable those skilled in the art to better understand the technical solutions of this utility model, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort should fall within the protection scope of this utility model.

[0101] It should be noted that when an element is referred to as being "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only embodiments.

[0102] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0103] like Figures 1 to 6 , Figure 9 and Figure 10 As shown, this utility model provides a reaction liquid carrier, comprising:

[0104] The first cavity structure 1 has a reaction cavity 11 formed inside it;

[0105] Heat transfer structure 2 is formed on the outer and / or inner side of reaction chamber 11, and heat transfer structure 2 is connected to first chamber structure 1.

[0106] The reaction liquid carrier provided in this embodiment has a reaction chamber 11 set in the first cavity structure 1 to carry the reaction liquid, so that the reaction liquid can be heated or cooled by the heat conduction or cold conduction of the first cavity structure 1 within the reaction chamber 11.

[0107] Furthermore, a heat transfer structure 2 is provided on the outside of the reaction chamber 11, on which a heat source device or a cold source device can be attached. Through the connection between the first chamber structure 1 and the heat transfer structure 2, heat or cold energy is transferred to the first chamber structure 1 to change its temperature, thereby changing the temperature of the reaction liquid in the reaction chamber 11. A heat transfer structure 2 is also provided on the inside of the reaction chamber 11, on which the heat or cold energy received by the first chamber structure 1 is transferred to the reaction chamber 11 to change the temperature of the reaction liquid in the reaction chamber 11. The heat transfer structure 2 on the outside of the reaction chamber 11 provides a mounting surface for the heat source device and the cold source device, while the heat transfer structure 2 on the inside of the reaction chamber 11 increases the contact area between the reaction liquid in the reaction chamber 11 and the heat conduction structure or component, further improving the temperature control efficiency of the reaction liquid.

[0108] Specifically, in this embodiment, the first cavity structure 1 is generally cubic; in other embodiments, the shape of the first cavity structure 1 is not specifically limited. In this embodiment, please refer to the relevant references. Figure 3 and Figure 9 As shown, the inner wall of the first cavity structure 1 divides into a reaction chamber 11 located inside the first cavity structure 1. In other embodiments, there is no limitation on the number of outer walls of the first cavity structure 1, nor is there any specific limitation on the formation conditions of the reaction chamber 11. In this embodiment, the first cavity structure 1 and the heat transfer structure 2 are preferably integrally formed. In the preferred embodiment, the heat transfer structure 2 is located outside the reaction chamber 11. The integrally formed structure allows the heat source / cold source received by the heat transfer structure 2 to be directly transferred from the inside of the material to the first cavity structure 1. In other embodiments, the first cavity structure 1 and the heat transfer structure 2 can also be welded or connected, and there is no specific limitation on this.

[0109] See also Figure 7 and Figure 8 As shown, in one specific embodiment, the outer wall of the first cavity structure 1 is connected to a heat transfer structure 2; in one embodiment, refer to [reference needed]. Figure 9 As shown, the heat transfer structure 2 can be provided extending outward from the outer wall of the first cavity structure 1 in a direction perpendicular to the outer wall of the first cavity structure 1; in another specific embodiment, refer to... Figure 31 As shown, the heat transfer structure 2 can also be attached to the outer wall of the first cavity structure 1; in another specific embodiment, see reference to Figure 32 As shown, the heat transfer structure 2 can also be an extension extending upwards or downwards from the outer wall of the first cavity structure 1 along the plane containing the outer wall of the first cavity structure 1; no specific limitation is made in this regard. In this embodiment, please refer to the accompanying references. Figure 9As shown, the surface of the heat transfer structure 2 is used to attach the heat output point of the heat source device or the cold output point of the cold source device, so as to transfer the heat energy provided by the heat source device or the cold energy provided by the cold source device to the first cavity structure 1, thereby changing the temperature of the first cavity structure 1 and thus changing the temperature of the reaction liquid in the reaction chamber 11.

[0110] In another specific embodiment, see reference. Figure 30 As shown, the heat transfer structure 2 can also be set on the inner sidewall of the first cavity structure 1 to increase the contact area between the reaction liquid and the heat conduction structure or component.

[0111] In yet another specific embodiment, see reference. Figure 2 and Figure 3 As shown, the heat transfer structure 2 can also be formed on both the outer and inner walls of the first cavity structure 1.

[0112] See also Figure 30 As shown, in a specific embodiment where a heat transfer structure 2 is connected to the inner wall of the first cavity structure 1, the heat transfer structure 2 can extend inward from the inner wall of the first cavity structure 1 in a direction perpendicular to the inner wall of the first cavity structure 1, or it can extend inward at a non-perpendicular angle to the inner wall of the first cavity structure 1. There is no specific limitation on the method of inward extension. In this embodiment, the heat transfer structure 2 is disposed inside the reaction chamber 11, and is connected to or integrally formed with the first cavity structure 1. The two opposing surfaces of the heat transfer structure 2 are in contact with the reaction liquid. The heat energy provided by the heat source device or the cold energy provided by the cold source device is transferred from the outer wall of the first cavity structure 1 to the reaction chamber 11 through the two opposing surfaces of the heat transfer structure 2. In this embodiment, the reaction liquid inside the reaction chamber 11 is not only temperature-controlled by the outer wall of the first cavity structure 1, but also by the heat transfer structure 2 located inside the reaction chamber 11.

[0113] See also Figure 10 As shown, in an embodiment where the heat transfer structure 2 is formed on both the outer and inner walls of the first cavity structure 1, a portion of the heat transfer structure 2 is formed on the outer wall of the first cavity structure 1, and another portion of the heat transfer structure 2 is formed on the inner wall of the first cavity structure 1. The heat transfer structure 2 located on the outer side of the first cavity structure 1 is used to attach a cold source device or a heat source device, and the heat transfer structure 2 located on the inner side of the first cavity structure 1 is used to transfer heat energy or cold energy, and divide the reaction chamber 11 into smaller cavities to facilitate improving the temperature control rate.

[0114] like Figures 1 to 5 As shown, in one specific embodiment, the heat transfer structure 2 includes at least one heat transfer plate 21, which extends outward from the outside of the reaction chamber 11.

[0115] The reaction liquid carrier provided in this embodiment, by providing a heat transfer plate 21 on the outside of the reaction chamber 11, allows the first chamber structure 1 to contact the heat source or cold source device through the heat transfer plate 21, thereby achieving the effect of regulating the temperature of the reaction liquid in the reaction chamber 11 by transferring cold or hot energy. Furthermore, by using an outwardly extending heat transfer plate 21, the size of the first chamber structure 1 can be eliminated from the limitation of the size of the heat source or cold source device, allowing the volume of the first chamber structure 1 to be further reduced, that is, the volume of the reaction chamber 11 can be further reduced, thereby increasing the heating and cooling rate of the reaction liquid under the influence of the heat source or cold source device of the same power. Furthermore, the heat transfer plate 21 extends outward from the outside of the reaction chamber 11, which can avoid the problem of uneven heating of the outer walls of the first chamber structure 1 due to different distances from the contact points of the heat source and cold source devices, thus improving the heating uniformity of the reaction chamber 11.

[0116] Specifically, in this embodiment, the first cavity structure 1 is generally a cube. In other embodiments, the shape of the first cavity structure 1 can also be a cylinder. There is no specific limitation on the specific shape of the first cavity structure 1. In this embodiment, the heat transfer plate 21 and the first cavity structure 1 are preferably integrally formed. In other embodiments, the heat transfer plate 21 can also be welded to the outer wall of the first cavity structure 1.

[0117] See also Figure 7 and Figure 8 As shown, in this embodiment, the heat transfer plate 21 is disposed on the outside of the reaction chamber 11, that is, the heat transfer plate 21 extends outward from the outer wall of the first cavity structure 1. In this embodiment, the heat transfer plate 21 extends outward in the horizontal direction, and the connection position between the heat transfer plate 21 and the first cavity structure 1 is located at the middle height of the outer wall of the first cavity structure 1, so that the top wall and bottom wall of the first cavity structure 1 are at the same distance from the heat transfer plate 21, which is conducive to the uniform temperature change of the inner wall of the first cavity structure 1 and also makes the temperature change of the reaction liquid in the reaction chamber 11 more balanced. In other embodiments, there is no specific limitation on the specific position of the heat transfer plate 21 connected to the first cavity structure 1.

[0118] In this embodiment, the heat transfer plate 21 extends outward from the outside of the reaction chamber 11, as shown in the reference. Figure 1 and Figure 2 As shown, in one specific embodiment, the heat transfer plate 21 can be a separate plate extending outwards, meaning the thickness of the heat transfer plate 21 is less than the height of the sidewall of the first cavity structure 1, and the heat transfer plate 21 is formed on or connected to one sidewall of the first cavity structure 1; in another specific embodiment, see reference to... Figure 3 and Figure 11As shown, the heat transfer plate 21 can also be a plate structure formed by extending the sidewall of the first cavity structure 1 outward as a whole, that is, the thickness of the heat transfer plate 21 is the same as the height of the sidewall of the first cavity structure 1, and there is no specific limitation on this. In other embodiments, there is no specific limitation on this.

[0119] In this embodiment, the number of heat transfer plates 21 is not specifically limited. In one embodiment, there are two heat transfer plates 21, which are arranged on opposite sides of the first cavity structure 1. In another embodiment, see [reference needed]. Figure 5 and Figure 6 As shown, the number of heat transfer plates 21 can also be three, four or more, with multiple heat transfer plates 21 arranged around the outer side wall of the first cavity structure 1; in another embodiment of this document, two heat transfer plates 21 can also be arranged at intervals along the height direction of the first cavity structure 1 on the same outer side wall of the first cavity structure 1. There are no specific restrictions on the specific arrangement of the heat transfer plates 21, which are limited to providing a mounting surface for the heat source device or the cold source device.

[0120] like Figures 1 to 4 , Figures 7 to 10 As shown, in one specific embodiment, there are two heat transfer plates 21, which are disposed on opposite sides of the first cavity structure 1.

[0121] In this embodiment, the reaction liquid carrier is provided with two heat transfer plates 21. A heat source device is attached to one heat transfer plate 21, and a cold source device is attached to the other. That is, heat energy is transferred through one heat transfer plate 21, and cold energy is transferred through the other, facilitating temperature control of the first cavity structure 1. Furthermore, by placing the two heat transfer plates 21 on opposite sides of the first cavity structure 1, temperature uniformity is achieved. Specifically, during the cooling process, the heat source device is first turned off, and the temperature of the heat transfer plate 21 connected to the heat source device naturally decreases. The temperature drops further; by turning on the cold source equipment, the heat transfer plate 21 connected to the cold source equipment transfers the cold source from the heat transfer plate 21 to the first cavity structure 1, and gradually transfers it to the heat transfer plate 21 connected to the heat source equipment. This allows the temperature of the reaction solution to drop faster, avoiding the problem that when the cold source equipment and the heat source equipment are connected to the same heat transfer plate 21, the cold source equipment needs to cool down the heat transfer plate 21 at a higher temperature before cooling down the first cavity structure 1, resulting in a longer cooling time for the first cavity structure 1 and an inability to quickly cool down the reaction solution, thus affecting the PCR annealing efficiency.

[0122] like Figure 3 , Figure 4 and Figure 10As shown, in one specific embodiment, the heat transfer structure 2 includes at least one intermediate plate 22, which is disposed inside the reaction chamber 11 and divides the reaction chamber 11 into at least two temperature control chambers 111.

[0123] The reaction liquid carrier provided in this embodiment divides the reaction chamber 11 into multiple temperature-controlled chambers 111 through an intermediate plate 22 disposed inside the reaction chamber 11. That is, the volume space within the reaction chamber 11 can be divided into multiple partitions through the intermediate plate 22 and the inner wall of the first chamber structure 1, resulting in at least two temperature-controlled chambers 111. This allows for a smaller liquid volume in each temperature-controlled chamber 111. The smaller liquid volume allows for faster temperature rise and fall when subjected to heat or cold energy conducted by the first chamber structure 1, achieving efficient temperature control. Simultaneously, the smaller liquid volume also prevents the heat transfer efficiency of the first chamber structure 1 from being affected by the internal structure of the liquid. To overcome the limitations of heat conduction efficiency and achieve high-efficiency heat conduction, this invention places an intermediate plate 22 inside the reaction chamber 11. This plate transfers the heat energy provided by the heat source device and the cold energy provided by the cold source device from the outer wall of the first chamber structure 1 to the reaction chamber 11. This allows the reaction liquid inside the reaction chamber 11 to be heated or cooled not only by the outer wall of the first chamber structure 1 but also by the intermediate plate 22 located inside the reaction chamber 11. This increases the heating and cooling rate of the reaction liquid, allowing a smaller volume of reaction liquid to contact a larger heating or cooling surface. This makes the temperature control efficiency of the heat transfer structure 2 for the reaction liquid higher, which in turn improves the measurement efficiency of qPCR.

[0124] In one specific embodiment, multiple intermediate plates 22 are equally spaced along the height direction of the reaction chamber 11, and the multiple intermediate plates 22 extend inward from the inner wall of the first cavity structure 1 in a direction perpendicular to the inner wall of the first cavity structure 1, so as to divide the reaction chamber 11 into multiple temperature control chambers 111 with the same volume. In another embodiment, the multiple intermediate plates 22 can also be equally spaced along the length direction of the reaction chamber 11, and the multiple intermediate plates 22 extend inward from the inner wall of the first cavity structure 1 in a direction perpendicular to the inner wall of the first cavity structure 1. In other embodiments, the intermediate plates 22 can also extend inward at a non-perpendicular angle with the inner wall of the first cavity structure 1, and the size of the angle between the intermediate plates 22 and the inner wall of the first cavity structure 1 is not specifically limited.

[0125] See also Figure 3 As shown, in this embodiment, the heat transfer structure 2 has both an intermediate plate 22 and a heat transfer plate 21. That is, in this embodiment, there is both a heat transfer plate 21 connected to the cold source device and the heat source device, and an intermediate plate 22 that divides the reaction chamber 11 into multiple temperature control chambers 111.

[0126] In another embodiment, see reference Figure 30As shown, the heat transfer structure 2 can also have only an intermediate plate 22 disposed inside the reaction chamber 11. The specific structure of the heat transfer structure 2 can be selected according to the size and power of the cold source and heat source devices provided in the actual qPCR process. In this embodiment, when the power and size of the cold source and heat source devices are small, only the intermediate plate 22 can be disposed, and the cold source and heat source devices can be attached to the outer wall or outer top wall of the first chamber structure 1. This structure is beneficial to improving the heat transfer efficiency of the cold source and heat source devices to the first chamber structure 1, avoiding the need for the cold source and heat source devices to be transferred to the first chamber structure 1 again through the heat transfer plate 21. It is also beneficial to quickly regulate the liquid temperature in multiple temperature control chambers 111 and avoid the heat transfer rate of the material of the heat transfer plate 21 affecting the overall heat transfer rate of the reaction liquid carrier.

[0127] In yet another embodiment, see reference. Figure 10 As shown, when the power and size of the cold source and heat source equipment are large, a heat transfer plate 21 can be set up, and the cold source and heat source equipment can be attached to the heat transfer plate 21. In this case, the heat transfer rate of the material of the heat transfer plate 21 has a low impact on the overall heat transfer rate of the reaction liquid carrier. In this embodiment, if the volume of the reaction chamber 11 is small, refer to the following... Figure 1 As shown, the intermediate plate 22 can be omitted; if the volume of the reaction chamber 11 is large, refer to [reference needed]. Figure 2 and Figure 3 As shown, intermediate board 22 needs to be set.

[0128] The choice of which embodiment to use depends on the required PCR replication efficiency, taking into account factors such as the height and volume of the reaction chamber 11, and the power and size of the cold and heat source devices.

[0129] Specifically, in this embodiment, there are multiple intermediate plates 22. In other embodiments, the number of intermediate plates 22 may be one; there is no specific limitation. In this embodiment, the intermediate plate 22 divides the reaction chamber 11 into multiple temperature-controlled chambers 111. These multiple temperature-controlled chambers 111 are interconnected, allowing the reaction solution to flow back and forth within each temperature-controlled chamber 111. This results in a more uniform temperature of the reaction solution within each temperature-controlled chamber 111, leading to better qPCR replication. Furthermore, the interconnected temperature-controlled chambers 111 facilitate more convenient control of the reaction solution during the injection process. The cavity 11 is used for venting, which avoids the need to vent each temperature control cavity 111 individually during liquid injection, thus improving venting efficiency. In other embodiments, the multiple temperature control cavities 111 may not be connected, and there is no specific limitation on this. In this embodiment, the intermediate plate 22 divides the reaction cavity 11 into multiple temperature control cavities 111 spaced apart along a fixed direction. The two sides of the intermediate plate 22 are respectively arranged for two adjacent temperature control cavities 111. In this embodiment, the multiple temperature control cavities 111 have the same volume. In other embodiments, the multiple temperature control cavities 111 may have different volumes, and there is no specific limitation on this.

[0130] like Figures 16 to 19 , Figure 26 As shown, in one specific embodiment, multiple temperature control chambers 111 are isolated and arranged. The first chamber structure 1 has multiple liquid inlets 12 and multiple liquid outlets 13, and the multiple liquid inlets 12 and multiple liquid outlets 13 are respectively connected to each temperature control chamber 111.

[0131] The reaction liquid carrier provided in this embodiment isolates the multiple temperature-controlled chambers 111 divided into the reaction chamber 11, meaning the multiple temperature-controlled chambers 111 are not interconnected. This facilitates individual heating or cooling of the reaction liquid, avoids the flow of the reaction liquid, and achieves a faster temperature control effect. Furthermore, each temperature-controlled chamber 111 is connected to an inlet 12 and an outlet 13, allowing liquid to be added to each temperature-controlled chamber 111 separately. This avoids the problem of incomplete filling of the temperature-controlled chamber 111 with air pockets due to the isolation of each temperature-controlled chamber 111, and also avoids the problem of reduced contact area between the inner wall of the first chamber structure 1 and the intermediate plate 22 and the reaction liquid due to air pockets.

[0132] Specifically, in this embodiment, the inlet 12 and outlet 13 are respectively disposed on opposite sidewalls of the first cavity structure 1; in this embodiment, two heat transfer plates 21 are disposed on the outer side of the first cavity structure 1, and the two heat transfer plates 21 are disposed on opposite sidewalls without inlet 12 and outlet 13. In other embodiments, the position of the heat transfer plates 21 is not specifically limited; in other embodiments, refer to the relevant references. Figure 30As shown, the heat transfer plate 21 may not be provided on the outside of the first cavity structure 1, and the liquid inlet 12 and outlet 13 may be provided on any opposite sidewall of the first cavity structure 1.

[0133] In this embodiment, the arrangement of the multiple temperature control cavities 111 can be divided into a stacked embodiment and a planar arrangement embodiment. In the stacked embodiment, the multiple temperature control cavities 111 can be stacked along the height direction of the first cavity structure 1. In the planar arrangement embodiment, the multiple temperature control cavities 111 can also be arranged at intervals along the same plane. The "stacked embodiment" and "planar arrangement embodiment" mentioned below refer to the above two arrangement methods.

[0134] In one embodiment of the stacked implementation, see reference to Figure 2 , Figure 16 , Figures 19 to 25 As shown, on the other sidewalls of the first cavity structure 1 without inlet 12 and outlet 13, a light-transmitting inner wall 14, an incident area 15, and a photometric area 16 can be provided, so that the irradiation device can directly irradiate each temperature-controlled cavity 111 from the sidewall of the first cavity structure 1, and the photometric device can directly obtain the fluorescence signal of the reaction liquid in each temperature-controlled cavity 111 from the sidewall of the first cavity structure 1; in another embodiment of the stacked embodiment, refer to Figure 17 and Figure 18 As shown, a light-transmitting inner wall 14, an incident area 15, and a photometric area 16 can also be provided on the top or bottom wall of the first cavity structure 1.

[0135] In one embodiment of the planar arrangement, see reference to Figure 26 and Figure 28 As shown, a light-transmitting inner wall 14, an incident area 15, and a photometric area 16 can be provided on the top or bottom wall of the first cavity structure 1, so that the irradiation device can directly irradiate each temperature control cavity 111 from the top wall of the first cavity structure 1, and the photometric device can directly obtain the fluorescence signal of the reaction liquid in each temperature control cavity 111 from the bottom wall of the first cavity structure 1.

[0136] like Figure 20 , Figure 22 , Figure 23 and Figure 25 As shown, in one specific embodiment, the outlet 13 is provided with a one-way structure 5 for gas passage only.

[0137] The reaction liquid carrier provided in this embodiment can prevent the reaction liquid from leaking out of the outlet 13 by setting a one-way structure 5 at the outlet 13. This can avoid the problem that the reaction liquid leaks out from the outlet 13 at the lower height or the outlet 13 near the injection port when each temperature control cavity 111 is connected to an outlet 13, which would make it difficult to fill each temperature control cavity 111.

[0138] Specifically, in this embodiment, the unidirectional structure 5 can be a breathable but waterproof membrane layer. The breathable but waterproof membrane layer of the unidirectional structure 5 in this embodiment can block large molecules such as liquids, allowing only small molecules such as gases to pass through. In other embodiments, there are no specific restrictions on the way and structure of the unidirectional structure 5 blocking liquids.

[0139] In this embodiment, please refer to the following: Figure 20 , Figure 22 , Figure 23 and Figure 25 As shown, in one embodiment of the stacked embodiment, a one-way structure 5 for gas passage only can be provided at each outlet 13; in one embodiment of the planar arrangement embodiment, refer to Figure 26 and Figure 28 As shown, a one-way structure 5 for gas passage can also be provided at each outlet 13; in other embodiments, the one-way structure 5 may not be provided, and there are no specific restrictions on this.

[0140] like Figures 20 to 25 , Figure 27 and Figure 28 As shown, in one specific embodiment, multiple temperature control cavities 111 are connected sequentially along their stacking direction. The first cavity structure 1 has at least one liquid inlet 12 and at least one outlet 13, and the at least one liquid inlet 12 and at least one outlet 13 are respectively connected to the multiple temperature control cavities 111.

[0141] The reaction liquid carrier provided in this embodiment divides the reaction chamber 11 into multiple temperature-controlled chambers 111 and connects them. That is, the reaction liquid in the multiple temperature-controlled chambers 111 can circulate with each other. During the heating or cooling process of the reaction liquid in the temperature-controlled chambers 111, when the temperature of the reaction liquid near the heat source or cold source is different from that of the reaction liquid far away from the heat source or cold source, the reaction liquid can also achieve heat exchange by flowing back and forth in each temperature-controlled chamber 111 due to the difference in the density of the hot and cold liquids. This makes the temperature of the reaction liquid in each temperature-controlled chamber 111 more uniform and improves the replication effect of qPCR. Multiple temperature-controlled chambers 111 are sequentially connected along their arrangement direction through at least one inlet 12 and at least one outlet 13. In a specific embodiment, only one inlet 12 and one outlet 13 can be provided, meaning that liquid can be added through only one inlet 12 and discharged or vented through only one outlet 13. This avoids the cumbersome and inefficient liquid addition process caused by adding liquid through multiple inlets 12, and the problem of needing a special pipette for uniform liquid addition, thus improving the efficiency of qPCR.

[0142] In the stacked embodiment, multiple temperature control cavities 111 can be stacked along the height direction of the first cavity structure 1, wherein:

[0143] In one embodiment, see reference Figure 1 , Figure 4 , Figure 20 , Figures 22 to 25 As shown, there is one outlet 13 and one inlet 12. The inlet 12 is located on the top wall of the first cavity structure 1 and is connected to the temperature control cavity 111 located at the top of the first cavity structure 1. The outlet 13 is connected to the temperature control cavity 111 located at the bottom of the first cavity structure 1. In a specific embodiment, a one-way structure 5 for gas passage is provided at the outlet 13. That is, the one-way structure 5 can be a gas-permeable but water-permeable membrane or other similar structures, and there are no specific limitations. In this embodiment, the liquid enters the reaction cavity 11 from the inlet 12. Due to the influence of gravity, the liquid fills each temperature control cavity 111 from bottom to top. In the embodiment where the outlet 13 is provided with a one-way structure 5, the liquid will not flow out from the outlet 13.

[0144] In another embodiment, see reference Figure 21 As shown, there are multiple outlets 13, each of which is connected to a temperature control chamber 111. That is, there is one inlet 12 and multiple outlets 13. The liquid enters the reaction chamber 11 from the inlet 12. Due to gravity, the liquid fills each temperature control chamber 111 from bottom to top. In a specific embodiment, a one-way structure 5 for gas passage is provided at the outlet 13 to prevent liquid overflow and improve the utilization rate of the liquid.

[0145] In another embodiment, see reference Figure 27 and Figure 28 As shown, multiple temperature control chambers 111 can also be arranged at intervals along the same plane. The liquid inlet 12 is located on the top wall of the first chamber structure 1 and is connected to the temperature control chamber 111 located at one end of the multiple temperature control chambers 111. The outlet 13 is connected to the temperature control chamber 111 located at the other end of the multiple temperature control chambers 111. In other embodiments, multiple outlets 13 can also be provided, and each outlet 13 is connected to each temperature control chamber 111. In other embodiments, one outlet 13 can be connected to one temperature control chamber 111, or multiple outlets 13 can be connected to one temperature control chamber 111. In other embodiments, a one-way structure 5 for gas passage can also be provided at the outlet 13. In other embodiments, there are no specific restrictions on the arrangement of the liquid inlet 12 and the outlet 13, as long as the arrangement of the liquid inlet 12 and the outlet 13 can fill the temperature control chamber 111 with the reaction liquid.

[0146] like Figure 23 and Figure 27 As shown, in one specific embodiment, the intermediate plate 22 and the inner wall of the first cavity structure 1 are divided to form a liquid passage 3, and two adjacent temperature control cavities 111 are connected through the liquid passage 3.

[0147] The liquid passage 3 of the reaction liquid carrier provided in this embodiment is formed by dividing the end of the intermediate plate 22 with the inner wall of the first cavity structure 1. That is, the adjacent temperature control cavities 111 are connected through the liquid passage 3 located at one end of the intermediate plate 22. The liquid passage 3 is formed on the inner wall of the first cavity structure 1, so that there is no structure that obstructs the flow of liquid in the flow channel, which reduces the structural complexity, facilitates the flow of the reaction liquid, reduces the probability of air cavity generation in the reaction cavity 11, facilitates filling each temperature control cavity 111, and also makes the temperature distribution uniform through the flow of the reaction liquid in each temperature control cavity 111.

[0148] Specifically, in this embodiment, the intermediate plate 22 extends inward along a direction perpendicular to the inner wall of the first cavity structure 1, and a liquid passage 3 is formed between one side of the intermediate plate 22 and the inner wall of the first cavity structure 1, while the remaining sides of the intermediate plate 22 are sealed to the inner wall of the first cavity structure 1; in the stacked embodiment, refer to the following... Figure 23 As shown, multiple temperature control chambers 111 can be stacked along the height direction of the first chamber structure 1. An intermediate plate 22 is horizontally inserted into the reaction chamber 11. The liquid passage 3 opens vertically, and its length is the same as the thickness of the intermediate plate 22. In the second embodiment, refer to... Figure 3 and Figure 27 As shown, multiple temperature control chambers 111 can also be arranged at intervals along the same plane. The intermediate plate 22 is horizontally arranged in the reaction chamber 11. The opening direction of the liquid passage 3 is horizontal. The length of the liquid passage 3 is consistent with the width of the intermediate plate 22. The thickness of the liquid passage 3 is consistent with the thickness of the intermediate plate 22 and the height of the reaction chamber 11. In other embodiments, there are no specific restrictions on the arrangement of the liquid passage 3.

[0149] like Figures 20 to 22 , Figure 24 , Figure 25 and Figure 28 As shown, in one specific embodiment, the intermediate plate 22 has a liquid passage 3, which is connected to two adjacent temperature control chambers 111.

[0150] The reaction liquid carrier provided in this embodiment has a liquid passage 3 opened on the intermediate plate 22. That is, the adjacent temperature control chambers 111 are connected through the liquid passage 3 on the intermediate plate 22, which further increases the area of ​​the intermediate plate 22 in contact with the reaction liquid in each temperature control chamber 111, improves the temperature control efficiency of each temperature control chamber 111, and enables the temperature of the reaction liquid in each temperature control chamber 111 to be raised or lowered more quickly.

[0151] Specifically, in this embodiment, the liquid passage 3 is generally a through circular hole, and the openings at both ends of the liquid passage 3 are respectively connected to two adjacent temperature control chambers 111; in one embodiment, refer to Figure 20 , Figure 21 and Figure 28As shown, the liquid passage 3 is formed at the exact center of the intermediate plate 22. In another embodiment, see reference 3. Figure 22 , Figure 24 and Figure 25 As shown, the liquid passage 3 can also be formed at one end or both ends of the intermediate plate 22, without specific limitation. In the embodiment where the liquid passage 3 is formed at the exact center of the intermediate plate 22, please refer to the following... Figure 21 and Figure 28 As shown, the first cavity structure 1 can be connected to a liquid inlet 12 and multiple outlets 13. Each temperature control cavity 111 has two outlets 13 connected to its two ends respectively. In this embodiment, the reaction liquid flows into and fills each temperature control cavity 111 through the liquid passage 3. Each temperature control cavity 111 exhausts gas outward through the two outlets 13. In this embodiment, the outlet 13 can be equipped with a one-way structure 5 for gas passage only, depending on the requirements. This embodiment can further avoid the generation of gas cavities and minimize the flow distance of each flow channel.

[0152] like Figures 22 to 25 , Figure 27 As shown, in one specific embodiment, multiple temperature control chambers 111 are connected end to end through a liquid passage 3.

[0153] The reaction liquid carrier provided in this embodiment can fill each temperature control cavity 111 by sequentially connecting the beginning and end of each temperature control cavity 111 through only one liquid inlet 12. The sequential connection method can also avoid the generation of dead flow paths and backflow paths of liquid, further avoid the generation of air chambers in the reaction cavity 11, and help to achieve the effect of filling the entire temperature control cavity 111 with reaction liquid.

[0154] Specifically, in this embodiment, adjacent temperature control chambers 111 are connected by a liquid passage 3 located at one end of the intermediate plate 22. The liquid passages 3 adjacent to each temperature control chamber 111 are located at opposite ends of each temperature control chamber 111 along the arrangement direction. That is, multiple temperature control chambers 111 arranged together form an S-shaped flow channel, which is beneficial to the flow of the reaction liquid during the injection process. In this embodiment, the liquid passage 3 is formed by dividing the intermediate plate 22 and the inner wall of the first cavity structure 1. In other embodiments, the liquid passage 3 can also be opened at one end of the intermediate plate 22, and there is no specific limitation on this.

[0155] like Figure 4 and Figure 6 As shown, in one specific embodiment, multiple temperature control chambers 111 are stacked. The liquid inlet 12 is connected to the temperature control chamber 111 located at the top of the reaction liquid carrier, and the outlet 13 is connected to the temperature control chamber 111 located at the bottom of the reaction liquid carrier. The first chamber structure 1 also has an exhaust chamber 6. One end of the exhaust chamber 6 is connected to the outside, and the other end of the exhaust chamber 6 is connected to the outlet 13.

[0156] The reaction liquid carrier provided in this embodiment, by stacking multiple temperature-controlled chambers 111, can increase the number of temperature-controlled chambers 111 and reduce the volume and thickness of each temperature-controlled chamber 111 while maintaining the overall thickness of the reaction chamber 11, thereby further improving the temperature control efficiency of each temperature-controlled chamber 111. Furthermore, the stacked temperature-controlled chambers 111, with each chamber exposing the same side, ensure that the light emitted by the irradiation device can enter each temperature-controlled chamber 111, enabling the reaction liquid within each chamber to produce a fluorescence effect and complete the photometric step. This avoids the problem of light slits or inability to penetrate due to the reduced thickness of the temperature-controlled chambers 111, thus preventing the light from being too small to measure light. Moreover, by restricting the positions of the inlet 12 and outlet 13 of the stacked temperature-controlled chambers 111, gravity allows the reaction liquid to automatically fill each temperature-controlled chamber 111, improving the injection efficiency of the reaction liquid carrier. Furthermore, the design of the exhaust chamber 6 can improve the exhaust efficiency of the reaction liquid carrier and avoid the problem that the reaction liquid cannot fill the entire temperature control chamber 111 due to the outlet 13 being located at the bottom of the reaction liquid carrier.

[0157] Specifically, in this embodiment, multiple temperature control cavities 111 are stacked in a vertical direction. In other embodiments, multiple temperature control cavities 111 may also be stacked in a vertical direction with a certain horizontal offset or angular offset between adjacent temperature control cavities 111. There are no specific limitations on this.

[0158] In this embodiment, the liquid inlet 12 is located on the top wall of the first cavity structure 1, and the liquid inlet 12 is connected to the temperature control cavity 111 located at the top of the plurality of temperature control cavities 111. The outlet 13 is located on the bottom side wall of the first cavity structure 1, and the outlet 13 is connected to the temperature control cavity 111 located at the bottom of the plurality of temperature control cavities 111. In this embodiment, the exhaust cavity 6 is disposed through the first cavity structure 1, the upper end of the exhaust cavity 6 is connected to the outside from the top wall of the first cavity structure 1, and the lower end of the exhaust cavity 6 is connected to the outlet 13. In other embodiments, multiple outlets 13 may be provided, and the multiple outlets 13 are respectively connected to multiple temperature control cavities 111. All multiple outlets 13 are connected to the exhaust cavity 6. There is no specific limitation on the number and arrangement of the outlets 13. In other embodiments, the outlets 13 may also be provided with a one-way structure 5, and there is no specific limitation on this.

[0159] During the injection process in this embodiment, the reaction liquid is injected into the temperature control chamber 111 through the injection port located at the top of the reaction liquid carrier. The reaction liquid flows through the liquid passage 3 under gravity and fills each temperature control chamber 111. The reaction liquid is discharged into the exhaust chamber 6 through the outlet 13 located at the bottom of the reaction liquid carrier. When the liquid level in the exhaust chamber 6 is consistent with the height of the injection port located at the top of the reaction liquid carrier, each temperature control chamber 111 is filled with the reaction liquid. When the outlet 13 is provided with a one-way structure 5 that allows only gas passage, the reaction liquid does not flow into the exhaust chamber 6 from the outlet 13.

[0160] In another embodiment, the positions of outlet 13 and inlet 12 can be interchanged. Outlet 13 is located on the top wall of the first cavity structure 1 and is connected to the temperature control cavity 111 located at the top of the plurality of temperature control cavities 111. Inlet 12 is located on the bottom side wall of the first cavity structure 1 and is connected to the temperature control cavity 111 located at the bottom of the plurality of temperature control cavities 111. In this embodiment, one end of exhaust cavity 6 is connected to the outside, and the other end of exhaust cavity 6 is connected to inlet 12. During the liquid injection process in this embodiment, the reaction liquid is injected from the top of the reaction liquid carrier. One end of the exhaust chamber 6 is injected into the exhaust chamber 6, and the reaction liquid enters the inlet 12 through the other end of the exhaust chamber 6 by gravity. Then, it enters the temperature control chamber 111 located at the bottom of the reaction liquid carrier from the inlet 12. As the reaction liquid is continuously injected, the reaction liquid fills each temperature control chamber 111 through the liquid passage 3 and is discharged through the outlet 13 located at the top of the reaction liquid carrier. When the liquid level in the exhaust chamber 6 is consistent with the height of the outlet 13 located at the top of the reaction liquid carrier, each temperature control chamber 111 is filled with the reaction liquid. By using this injection method, it can be further ensured that no gas chamber is generated in each temperature control chamber 111.

[0161] like Figure 3 , Figure 11 , Figures 27 to 28 As shown, in one specific embodiment, multiple temperature control chambers 111 are arranged at intervals along the same plane, the liquid inlet 12 is connected to the temperature control chamber 111 located at one end of the multiple temperature control chambers 111, and the outlet 13 is connected to the temperature control chamber 111 located at the other end of the multiple temperature control chambers 111.

[0162] The reaction liquid carrier provided in this embodiment, by arranging multiple temperature-controlled cavities 111 at intervals along the same plane, can increase the number of temperature-controlled cavities 111 and reduce the volume and width of each temperature-controlled cavity 111 while keeping the overall width of the reaction cavity 11 unchanged, thereby further improving the temperature control efficiency of each temperature-controlled cavity 111. Furthermore, by arranging the temperature-controlled cavities 111 at intervals along the same plane and exposing the same top surface to the outside, it can ensure that the light emitted by the irradiation device can enter each temperature-controlled cavity 111, enabling the reaction liquid in each temperature-controlled cavity 111 to produce a fluorescence effect and complete the photometric step. This avoids the problem of light emitted by the irradiation device creating a light slit effect or being unable to enter due to the reduced width of the temperature-controlled cavity 111, and also avoids the problem of the light entrance being too small to measure light. Furthermore, the temperature control cavities 111, which are spaced apart along the same plane, can automatically fill each temperature control cavity 111 by relying on the liquid flow by restricting the positions of the liquid inlet 12 and the outlet 13. It is also possible to directly observe whether an air cavity is generated from the light-transmitting inner wall 14 at the top of the first cavity structure 1, which is beneficial to improving the liquid injection efficiency of the reaction liquid carrier.

[0163] Specifically, in this embodiment, please refer to the following: Figure 27 As shown, multiple temperature-controlled chambers 111 are spaced apart along one direction on the same horizontal plane. An inlet 12 is located on the top wall of the first chamber structure 1, and is connected to a temperature-controlled chamber 111 located at one end of the multiple temperature-controlled chambers 111. Similarly, an outlet 13 is located on the top wall of the first chamber structure 1, and is connected to a temperature-controlled chamber 111 located at the other end of the multiple temperature-controlled chambers 111. In the actual liquid injection process of this embodiment, the reaction liquid is injected into the temperature-controlled chamber 111 through the injection port located at the top of the reaction liquid carrier. After the reaction liquid fills each temperature-controlled chamber 111 through the liquid passage 3, it is discharged through the outlet 13. When the reaction liquid is discharged from the outlet 13, each temperature-controlled chamber 111 is filled with the reaction liquid. In other embodiments, please refer to the relevant documentation. Figure 28 As shown, multiple outlets 13 can also be provided, and each outlet 13 is connected to a multiple temperature control chamber 111. There are no specific restrictions on the number and arrangement of the outlets 13.

[0164] like Figure 26 and Figure 28 As shown, in one specific embodiment, multiple temperature control chambers 111 are arranged at intervals along the same plane, and there are multiple outlets 13, each of which is connected to one end of a multiple temperature control chamber 111.

[0165] The reaction liquid carrier provided in this embodiment, by setting multiple outlets 13, can realize the individual exhaust of each temperature control chamber 111, avoiding the problem of air chambers easily being generated in the reaction chamber 11 due to the structure of the reaction chamber 11 divided by the intermediate plate 22 or the return flow channel during the liquid injection process. This allows each temperature control chamber 111 to be filled more quickly, improving the liquid injection efficiency.

[0166] Specifically, in one embodiment of this document, please refer to the accompanying document. Figure 26 As shown, multiple temperature control chambers 111 are spaced apart and isolated from each other along the same plane. Multiple injection ports are connected to multiple temperature control chambers 111 respectively, and multiple outlets 13 are connected to multiple temperature control chambers 111 respectively. The outlets 13 and injection ports are connected to the opposite ends of each temperature control chamber 111 respectively. That is, during the injection process, the liquid is injected from the injection port, and the injection of the temperature control chamber 111 is completed when the reaction liquid flows out from the outlet 13.

[0167] In another embodiment of this paper, a liquid inlet 12 and multiple outlets 13 are provided. Multiple temperature control chambers 111 are spaced apart along the same plane and interconnected. A liquid passage 3 is formed on the same side of the multiple temperature control chambers 111. The liquid inlet 12 is located on the top wall of the first cavity structure 1 and is connected to the temperature control chamber 111 located at one end of the multiple temperature control chambers 111. Similarly, multiple outlets 13 are spaced apart on the top wall of the first cavity structure 1, and each outlet 13 is connected to each temperature control chamber 111. Each outlet 13 is connected to the side of each temperature control chamber 111 away from the liquid passage 3. In the actual liquid injection process of this embodiment, the reaction liquid is injected into the temperature control chamber 111 from the liquid injection port located at one end of the reaction liquid carrier. The reaction liquid fills each temperature control chamber 111 through the liquid passage 3 and is discharged through each outlet 13 of each temperature control chamber 111. When reaction liquid is discharged from each outlet 13, each temperature control chamber 111 is filled with reaction liquid.

[0168] In yet another embodiment of this document, please refer to [the relevant documentation]. Figure 28As shown, a liquid inlet 12 and multiple outlets 13 are provided. Multiple temperature control chambers 111 are spaced apart along the same plane and interconnected. A liquid passage 3 is formed at the center of the intermediate plate 22. The liquid inlet 12 is located on the top wall of the first cavity structure 1 and is connected to one end of the multiple temperature control chambers 111. The multiple outlets 13 are also spaced apart on the top wall of the first cavity structure 1. Each temperature control chamber 111 is connected to two outlets 13, which are located on opposite sides of each temperature control chamber 111. In the actual liquid injection process of this embodiment, the reaction liquid is injected into the temperature control chamber 111 from the injection port located at one end of the reaction liquid carrier. The reaction liquid fills each temperature control chamber 111 through the liquid passage 3 and is discharged through the two outlets 13 connecting each temperature control chamber 111. When all outlets 13 of all temperature control chambers 111 have reaction liquid discharged, each temperature control chamber 111 is filled with reaction liquid. In other embodiments, a one-way structure 5 can be provided at all outlets 13 to prevent liquid from being discharged from outlets 13 that are closer to inlet 12, thus failing to fill the temperature control chamber 111 that is farther from inlet 12.

[0169] like Figures 16 to 28 As shown, in one specific embodiment, the first cavity structure 1 has a light-transmitting inner wall 14 that exposes at least one temperature-controlled cavity 111, and the first cavity structure 1 also has an incident area 15, with the two ends of the incident area 15 corresponding to the light-transmitting inner wall 14 and the external arrangement of the first cavity structure 1, respectively.

[0170] The reaction liquid carrier provided in this embodiment, by providing a light-transmitting inner wall 14 and an incident area 15, allows the irradiation device to directly irradiate the temperature-controlled cavity 111 from the outside of the reaction liquid carrier. This avoids the need to remove the reaction liquid after each reaction, which would otherwise lead to low QPCR photometric efficiency. The incident area 15 exposes the reaction cavity 11 outwards, facilitating the irradiation device to penetrate the first cavity structure 1 through the incident area 15. Furthermore, the structure of the incident area 15 avoids the need for multiple irradiation channels, thus improving the photometric efficiency of each temperature-controlled cavity 111. The light-transmitting inner wall 14 exposes the temperature-controlled cavity 111 outwards and increases the area of ​​light that can be received by the temperature-controlled cavity 111. This improves the light intake of each temperature-controlled cavity 111 with a smaller volume, thereby increasing the photometric efficiency of the reaction liquid within each temperature-controlled cavity 111.

[0171] Specifically, in this embodiment, the incident area 15 is generally an opening on the outer wall of the first cavity structure 1. One end of the opening faces the outside of the first cavity structure 1, and the other end of the opening faces the light-transmitting inner wall 14 inside the first cavity structure 1. The irradiation device can irradiate the interior of the first cavity structure 1 from the incident area 15. In other embodiments, the incident area 15 can also be other structures formed by the first cavity structure 1 that expose the interior of the first cavity structure 1. The specific structure of the incident area 15 is not limited, but is limited to structures that can expose the interior of the first cavity structure 1 to the outside.

[0172] In this embodiment, the light-transmitting inner wall 14 is formed on the outer wall of the temperature control cavity 111. That is, the reaction liquid in the temperature control cavity 111 is exposed to the light irradiation range of the irradiation device through the light-transmitting inner wall 14 and the incident area 15. In this embodiment, please refer to the reference. Figures 16 to 18 , Figures 20 to 24 , Figures 26 to 28 As shown, the light-transmitting inner wall 14 can be a membrane structure covering the opening of the outer wall of the first cavity structure 1. In other embodiments, refer to [reference needed]. Figure 19 and Figure 25 As shown, the light-transmitting inner wall 14 can also be the outer wall of the temperature control cavity 111 made of light-transmitting material. There are no restrictions on the location and specific structure of the light-transmitting inner wall 14, except that the reaction liquid inside the temperature control cavity 111 is exposed. One side of the light-transmitting inner wall 14 is set facing the incident area 15, so that the reaction liquid inside the temperature control cavity 111, the light-transmitting inner wall 14, and the incident area 15 are on the same light path. The irradiation device irradiates the light-transmitting inner wall 14 from the incident area 15, and the light enters the temperature control cavity 111 from the light-transmitting inner wall 14, causing the reaction liquid to undergo a fluorescent reaction.

[0173] like Figure 19 and Figure 25 As shown, in a specific embodiment of this article, multiple temperature control cavities 111 are stacked vertically, and an intermediate plate 22 is inserted into the reaction chamber 11 in a horizontal or certain direction. In this embodiment, the temperature control cavity 111 is formed by the support member 7 supporting the temperature control cavity 111, the intermediate plate 22, and the inner wall of the first cavity structure 1. The incident area 15 is an opening on the side wall of the first cavity structure 1. The opening of the incident area 15 exposes the side wall of the multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the incident area 15.

[0174] In another embodiment of the stacked implementation, see reference to Figure 6 , Figure 19 and Figure 25As shown, the temperature control cavity 111 can also be formed by only the support member 7 and the intermediate plate 22 supporting the temperature control cavity 111. That is, the support member 7 is an annular strip structure. The incident area 15 is an opening on the side wall of the first cavity structure 1. The opening of the incident area 15 exposes the side wall of multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the incident area 15.

[0175] In yet another embodiment of the stacked implementation, see reference to Figure 16 , Figures 20 to 24 As shown, the temperature control cavity 111 can also be formed by dividing the intermediate plate 22 and the inner wall of the first cavity structure 1. The incident area 15 is an opening on the side wall of the first cavity structure 1, and the opening of the incident area 15 exposes the side wall of multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is a transparent film layer structure covering the opening of the incident area 15. In other embodiments, see reference to... Figure 17 As shown, the incident area 15 can also be an opening on the top wall of the first cavity structure 1. The opening of the incident area 15 exposes the top wall of the temperature control cavity 111 located on top of the multiple temperature control cavities 111. The light-transmitting inner wall 14 is a transparent film structure covering the opening of the incident area 15.

[0176] like Figure 6 and Figure 19 As shown, in a specific embodiment of the stacked embodiment, the temperature control cavity 111 is formed by the support member 7 supporting the reaction cavity 11, the inner wall of the first cavity structure 1, and the intermediate plate 22. In this embodiment, the support member 7 is an annular strip structure. The incident area 15 is an opening on the side wall of the first cavity structure 1. The opening of the incident area 15 exposes the side wall of multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the incident area 15.

[0177] See also Figures 26 to 28 As shown, in a specific embodiment of the planar arrangement, multiple temperature control chambers 111 are spaced apart along the same plane, and an intermediate plate 22 is inserted into the reaction chamber 11 in a horizontal or certain direction. In this embodiment, the temperature control chamber 111 is formed by the intermediate plate 22 and the inner wall of the first cavity structure 1. The incident area 15 is an opening on the top wall of the first cavity structure 1, and the opening of the incident area 15 exposes multiple temperature control chambers 111. The light-transmitting inner wall 14 is a transparent film layer structure covering the opening of the incident area 15. In other embodiments, there are no restrictions on the specific structural settings of the incident area 15 and the light-transmitting inner wall 14.

[0178] In another specific embodiment, the reaction chamber 11 does not have an intermediate plate 22, that is, the reaction chamber 11 is not divided into a temperature control chamber 111; in a specific embodiment, see reference. Figure 15As shown, the incident area 15 is an opening on the side wall of the first cavity structure 1, the reaction chamber 11 is formed by the support member 7 and the top and bottom walls of the first cavity structure 1, and the light-transmitting inner wall 14 is the side wall of the support member 7 facing the incident area 15; in another specific embodiment, refer to [reference needed]. Figure 12 and Figure 14 As shown, the incident area 15 is an opening on the side wall of the first cavity structure 1, the reaction chamber 11 is formed by dividing the outer wall of the first cavity structure 1, and the light-transmitting inner wall 14 is a transparent film structure covering the opening of the incident area 15; in another specific embodiment, see reference to Figure 13 As shown, the incident area 15 is an opening on the top wall of the first cavity structure 1, the reaction cavity 11 is formed by dividing the outer wall of the first cavity structure 1, and the light-transmitting inner wall 14 is a transparent film structure covering the opening of the incident area 15. In this embodiment, the light-measuring light shines from the incident area 15 onto the light-transmitting inner wall 14, and the light shines from the light-transmitting inner wall 14 into the reaction cavity 11.

[0179] like Figure 1 , Figure 2 and Figure 5 As shown, in one specific embodiment, the first cavity structure 1 further includes a photometering area 16, with the two ends of the photometering area 16 corresponding to the light-transmitting inner wall 14 and the external arrangement of the first cavity structure 1, respectively.

[0180] The reaction liquid carrier provided in this embodiment, by setting a photometric area 16, allows the photometric device to directly receive the fluorescence signal reflected from the temperature control cavity 111 through the photometric area 16. This avoids the need for a dedicated photometric channel, which would otherwise encroach on the arrangement space of the first cavity structure 1. It also avoids the problem of needing to remove the reaction liquid for measurement in existing devices. Setting the photometric area 16 exposes the interior of the first cavity structure 1 to the outside, making it easier for the photometric device to receive the fluorescence signals from the multiple temperature control cavities 111 inside the first cavity structure 1. At the same time, the use of a light-transmitting inner wall 14 increases the window area of ​​the fluorescence signal that can be reflected by the temperature control cavity 111, which is beneficial to improve the light output of each temperature control cavity 111 with a smaller temperature control cavity volume, that is, to improve the photometric efficiency of the reaction liquid in each temperature control cavity 111.

[0181] Specifically, in this embodiment, the photometric area 16 is generally an opening on the outer wall of the first cavity structure 1. One end of the opening faces the outside of the first cavity structure 1, and the other end faces the light-transmitting inner wall 14 inside the first cavity structure 1. The photometric device can receive the fluorescence signal reflected from the light-transmitting inner wall 14 from the photometric area 16. In other embodiments, the photometric area 16 can also be other structures formed by the first cavity structure 1 that expose the light-transmitting inner wall 14. The specific structure of the photometric area 16 is not limited, but is limited to structures that can expose the light-transmitting inner wall 14 to the outside. In this embodiment, refer to the reference... Figure 5As shown, the photometric area 16 and the incident area 15 can face the same light-transmitting inner wall 14. In other embodiments, the photometric area 16 and the incident area 15 can also face different light-transmitting inner walls 14. There is no specific limitation on this, and it can be adjusted according to the structural requirements of the reaction liquid carrier. In this embodiment, the light-transmitting inner wall 14 is formed on the outer wall of the temperature control cavity 111. That is, the reaction liquid in the temperature control cavity 111 is exposed to the field of view of the photometric device through the light-transmitting inner wall 14 and the photometric area 16. In this embodiment, one side of the light-transmitting inner wall 14 faces the photometric area 16, so that the reaction liquid in the temperature control cavity 111, the light-transmitting inner wall 14, the photometric area 16, and the photometric device are on the same optical path. The photometric light beam shines from the incident area 15 onto the light-transmitting inner wall 14, enters the reaction cavity 11 from the light-transmitting inner wall 14, and then exits the photometric area 16 from the same or different light-transmitting inner walls 14 and enters the photometric device to complete the photometric operation. In all embodiments and implementations of the reaction liquid carrier provided by this utility model, unless otherwise explained, the photometric principles and steps adopted in each embodiment and implementation are consistent with the above-described principles and steps.

[0182] In one specific embodiment of the stacked embodiments described herein, see also [link to relevant documentation]. Figure 19 As shown, multiple temperature control chambers 111 are stacked vertically, and an intermediate plate 22 is inserted into the reaction chamber 11 in a horizontal or certain direction. In this embodiment, the temperature control chamber 111 is formed by the support member 7, the intermediate plate 22, and the inner wall of the first cavity structure 1. The photometric area 16 is an opening on the side wall of the first cavity structure 1. The opening of the photometric area 16 exposes the side wall of the multiple temperature control chambers 111 on the same side. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the photometric area 16, that is, the support member 7 is made of a light-transmitting material.

[0183] In another embodiment of the stacked implementation, see reference to Figure 6 As shown, the temperature control cavity 111 can also be formed by only the support member 7 and the intermediate plate 22 used to support the temperature control cavity 111. The support member 7 is an annular strip structure. The photometering area 16 is an opening on the side wall of the first cavity structure 1. The opening of the photometering area 16 exposes the side wall of multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the photometering area 16. That is, the support member 7 is made of a light-transmitting material.

[0184] In yet another embodiment of the stacked implementation, see reference to Figure 2 and Figure 16As shown, the temperature control cavity 111 can also be formed by dividing the intermediate plate 22 and the inner wall of the first cavity structure 1. The photometering area 16 is an opening on the side wall of the first cavity structure 1, exposing the side wall of multiple temperature control cavities 111 on the same side. The light-transmitting inner wall 14 is a transparent film structure covering the opening of the photometering area 16. In other embodiments, the specific structural configuration of the photometering area 16 and the light-transmitting inner wall 14 is not limited. In other embodiments, the photometering area 16 can also be an opening on the top wall of the first cavity structure 1, exposing one temperature control cavity 111 located at the top of the multiple temperature control cavities 111. The light-transmitting inner wall 14 is a transparent film structure covering the opening of the photometering area 16.

[0185] In a specific embodiment of the planar arrangement, multiple temperature control chambers 111 are spaced apart along the same plane, and an intermediate plate 22 is inserted into the reaction chamber 11 in a horizontal or certain direction. In this embodiment, the temperature control chamber 111 is formed by the intermediate plate 22 and the inner wall of the first cavity structure 1. The photometering area 16 is an opening on the top wall of the first cavity structure 1, and the opening of the photometering area 16 exposes multiple temperature control chambers 111. The light-transmitting inner wall 14 is a transparent film layer structure covering the opening of the photometering area 16. In other embodiments, there are no restrictions on the specific structural settings of the photometering area 16 and the light-transmitting inner wall 14.

[0186] In yet another specific embodiment of this document, please refer to [the relevant documentation]. Figure 15 As shown, the reaction chamber 11 does not have an intermediate plate 22, that is, the reaction chamber 11 is not divided into a temperature control chamber 111. In this embodiment, the photometric area 16 is an opening on the side wall of the first cavity structure 1. The reaction chamber 11 is formed by the support member 7 for supporting the reaction chamber 11 and the top and bottom walls of the first cavity structure 1. The light-transmitting inner wall 14 is the side wall of the support member 7 facing the photometric area 16. In other embodiments, the photometric area 16 is an opening on the side wall of the first cavity structure 1. The reaction chamber 11 is formed by the outer wall of the first cavity structure 1. The light-transmitting inner wall 14 is a transparent film structure covering the opening of the photometric area 16. In other embodiments, the photometric area 16 is an opening on the bottom wall of the first cavity structure 1. The reaction chamber 11 is formed by the outer wall of the first cavity structure 1. The light-transmitting inner wall 14 is a transparent film structure covering the opening of the photometric area 16.

[0187] like Figure 1 , Figure 2 , Figure 4 , Figure 7 , Figure 8 , Figure 11 , Figure 13 , Figure 17 and Figure 18 As shown, in one specific embodiment, the first cavity structure 1 has two light-transmitting inner walls 14 arranged opposite to each other, and the photometric area 16 and the incident area 15 are respectively arranged corresponding to the two light-transmitting inner walls 14.

[0188] The reaction liquid carrier provided in this embodiment, by providing two oppositely arranged light-transmitting inner walls 14, that is, providing a direct light path for the light emitted from the irradiation device, can improve the intensity of the fluorescence signal that the photometric device can receive.

[0189] See also Figure 6 , Figure 15 and Figure 19 As shown, the temperature control cavity 111 can be formed by dividing the support member 7, the intermediate plate 22 and the inner wall of the first cavity structure 1. The temperature control cavity 111 can also be formed by dividing the support member 7 and the intermediate plate 22, that is, the support member 7 is an annular strip structure. In one embodiment of the above-mentioned temperature control cavity 111 structure type, the photometering area 16 and the incident area 15 are both openings opened on the side wall of the first cavity structure 1. The opening of the photometering area 16 and the opening of the incident area 15 are formed on the opposite side wall of the first cavity structure 1. The light-transmitting inner wall 14 is the side wall of the support member 7. Two light-transmitting inner walls 14 are also formed on the opposite side wall of the support member 7. The two light-transmitting inner walls 14 are respectively set to correspond to the opening of the photometering area 16 and the opening of the incident area 15.

[0190] In the embodiment where the temperature control cavity 111 is divided by the intermediate plate 22 and the inner wall of the first cavity structure 1, please refer to the following: Figure 1 , Figure 2 , Figure 4 , Figure 7 , Figure 8 As shown, both the metering area 16 and the incident area 15 are openings on the sidewalls of the first cavity structure 1. The openings of the metering area 16 and the incident area 15 are formed on opposite sidewalls of the first cavity structure 1. The two light-transmitting inner walls 14 are transparent film structures, respectively covering the openings of the metering area 16 and the incident area 15. In other embodiments, please refer to the reference... Figure 13 , Figure 17 and Figure 18 As shown, the photometering area 16 can also be an opening on the bottom wall of the first cavity structure 1, the incident area 15 is an opening on the top wall of the first cavity structure 1, and the opening of the photometering area 16 and the opening of the incident area 15 are arranged opposite to each other. The light-transmitting inner wall 14 is a transparent film structure that covers the opening of the photometering area 16.

[0191] like Figure 17 and Figure 18 As shown, in one specific embodiment, the intermediate plate 22 has a light-transmitting structure 8 facing two adjacent reaction chambers 11.

[0192] The reaction liquid carrier provided in this embodiment, by providing a light-transmitting structure 8 in the intermediate plate 22, enables light transmission between two adjacent temperature control cavities 111. This increases the light intensity in each temperature control cavity 111 when the irradiation device irradiates it, allowing for clearer observation of the fluorescence reaction of the reaction liquid and improving the overall photometric efficiency.

[0193] In this embodiment, the light-transmitting structure 8 consists of a light-transmitting hole and a film layer structure covering the light-transmitting hole. The light-transmitting hole is a hole that penetrates the intermediate plate 22. In other embodiments, the light-transmitting structure 8 can also be a transparent inner wall on the intermediate plate 22. The specific structure of the light-transmitting structure 8 is not limited.

[0194] like Figure 17 and Figure 18 As shown, in one specific embodiment, there are multiple intermediate plates 22, and the light-transmitting structures 8 of the multiple intermediate plates 22 are spaced apart along the opening direction of the incident area 15.

[0195] The reaction liquid carrier provided in this embodiment restricts the position of the light-transmitting structure 8 of the multiple intermediate plates 22, that is, restricts the light-transmitting structure 8 to be in the light path of the incident area 15, which can further improve the brightness in each temperature control cavity 111, enable clearer observation of the fluorescence reaction of the reaction liquid, and improve the overall photometric efficiency.

[0196] In this embodiment, the light-transmitting structure 8 of the intermediate plate 22 is disposed in the opening direction of the incident area 15. The light emitted from the irradiation structure passes through the light-transmitting inner wall 14 and the light-transmitting structure 8 from the incident area 15 and enters each temperature control cavity 111, which can increase the brightness in each temperature control cavity 111.

[0197] In a specific embodiment of the planar arrangement described herein, multiple temperature control chambers 111 are spaced apart along the same plane. An intermediate plate 22 is inserted into the reaction chamber 11 in a horizontal or certain direction. The temperature control chamber 111 is formed by the intermediate plate 22 and the inner wall of the first cavity structure 1. The incident area 15 is an opening on the side wall of the first cavity structure 1. The opening of the photometric area 16 exposes the temperature control chamber 111 located at one end of the multiple temperature control chambers 111. The light-transmitting inner wall 14 is a transparent film layer structure covering the opening of the incident area 15. The light-transmitting structure 8 of each intermediate plate 22 is arranged in the direction of the opening of the incident area 15 so that the light path can pass through the light-transmitting structure 8 to irradiate the temperature control chamber 111 located at one end of the multiple temperature control chambers 111 to the temperature control chamber 111 located at the other end of the multiple temperature control chambers 111.

[0198] In one specific embodiment of the stacked embodiments described herein, see also [link to relevant documentation]. Figure 17 and Figure 18As shown, multiple temperature control chambers 111 are stacked vertically, and intermediate plates 22 are inserted into the reaction chamber 11 in a horizontal or certain direction. The incident area 15 can also be an opening on the top wall of the first chamber structure 1. The opening of the incident area 15 exposes the temperature control chamber 111 located at the top of the multiple temperature control chambers 111. The light-transmitting inner wall 14 is a transparent film layer structure covering the opening of the incident area 15. The light-transmitting structure 8 of each intermediate plate 22 is set in the direction of the opening of the incident area 15 so that the light path can pass through the light-transmitting structure 8 to irradiate from the temperature control chamber 111 located at the top of the multiple temperature control chambers 111 to the temperature control chamber 111 located at the bottom of the multiple temperature control chambers 111. In other embodiments, the setting position of the light-transmitting structure 8 is not specifically limited.

[0199] like Figure 17 and Figure 18 As shown, in one specific embodiment, the surface of the intermediate plate 22 is arranged perpendicular to the opening direction of the incident area 15.

[0200] The reaction liquid carrier provided in this embodiment, by restricting the surfaces of multiple intermediate plates 22, that is, restricting the plane of the light-transmitting structure 8 to be perpendicular to the light path incident from the incident area 15, can further improve the brightness in each temperature control cavity 111, enabling clearer observation of the fluorescence reaction of the reaction liquid and improving the overall photometric efficiency.

[0201] Specifically, in this embodiment, the light-transmitting structure 8 can also be a transparent inner wall on the intermediate plate 22. The plate surface of the intermediate plate 22 is perpendicular to the opening direction of the incident area 15, that is, the transparent inner wall is perpendicular to the opening direction of the incident area 15, so that the light path will not be refracted when passing through the light-transmitting structure 8, which is beneficial to improving the overall brightness of the irradiation device irradiating multiple temperature control chambers 111.

[0202] like Figure 6 , Figure 15 , Figure 18 , Figure 19 and Figure 25 As shown, in one specific embodiment, the first cavity structure 1 also has a support member 7, which is connected between two adjacent intermediate plates 22, and the support member 7 and the two adjacent intermediate plates 22 divide to form a temperature control cavity 111.

[0203] The reaction liquid carrier provided in this embodiment, by providing a support member 7 within the first cavity structure 1, can improve the overall structural stability of the first cavity structure 1. Further details can be found in the accompanying reference. Figure 6 As shown, the use of support member 7 can avoid the outer wall of the first cavity structure 1 from being affected by temperature changes during the conduction of cold and heat energy, or the structural support being insufficient or the outer wall of the first cavity structure 1 softening due to high temperature. It also avoids the problems of volume change and shape deformation of each temperature control cavity 111 or reaction cavity 11 due to changes in the first cavity structure 1.

[0204] Specifically, in this embodiment, the support member 7 is generally an annular strip structure, wherein multiple temperature control chambers 111 are stacked vertically, and the intermediate plate 22 is inserted into the reaction chamber 11 in the horizontal direction or a certain direction. The top and bottom surfaces of the support member 7 are connected to the adjacent intermediate plates 22 respectively. The inner wall of the support member 7 and the intermediate plate 22 divide to form the temperature control chamber 111. In this embodiment, a liquid passage 3 is opened on the intermediate plate 22.

[0205] In other embodiments, there may be two supports 7, each a cubic structure, arranged opposite to each other. The two supports 7 are respectively positioned at two opposing openings formed by the intermediate plate 22 and the inner wall of the first cavity structure. The top and bottom surfaces of the supports 7 are respectively sealed to adjacent intermediate plates 22. The two left and right sides of the supports 7 are respectively sealed to the two opposing inner walls of the first cavity structure 1. The inner sides of the supports 7, the intermediate plate 22, and the inner walls of the first cavity structure 1 form a temperature control cavity 111. In one embodiment, a liquid passage 3 is formed between the intermediate plate 22 and the inner wall of the first cavity structure 1. In another embodiment, the liquid passage 3 may be located on the intermediate plate 22. The material of the supports 7 needs to have good compatibility with the PCR reaction solution. In this embodiment, the supports 7 may be made of transparent materials such as UV adhesive. In other embodiments, the supports 7 may also be made of transparent PP / PC plastic. The light-transmitting inner wall 14 is formed on the supports 7.

[0206] like Figure 15 , Figure 19 and Figure 25 As shown, in one specific embodiment, a light-transmitting inner wall 14 is formed on the side wall of the support member 7, and the photometering area 16 and the incident area 15 are disposed facing at least one light-transmitting inner wall 14 of the support member 7.

[0207] The reaction liquid carrier provided in this embodiment, by forming the light-transmitting inner wall 14 on the side wall of the support member 7, facilitates the irradiation device and the photometer to quickly measure the temperature control cavity 111 through the photometer area 16 and the incident area 15, which is beneficial to improving the photometering efficiency of qPCR.

[0208] In this embodiment, multiple temperature control cavities 111 are stacked vertically, and intermediate plates 22 are inserted into the reaction cavity 11 in a horizontal or certain direction. The support member 7 is generally annular, and the top and bottom surfaces of the support member 7 are connected to the adjacent intermediate plates 22 respectively. The inner sidewall of the support member 7 and the intermediate plates 22 divide to form the temperature control cavity 111. The support member 7 is made of transparent material. That is, when the first cavity structure 1 is provided with the incident area 15 and the photometric area 16, photometric measurements can be performed relative to the support member 7 from any incident port and photometric port, and the brightness in the temperature control cavity 111 can be ensured, which is beneficial to improving photometric efficiency.

[0209] like Figures 12 to 25 As shown, in one specific embodiment, the first cavity structure 1 has an upper plate 171 and a lower plate 172 stacked together. The upper plate 171 and the lower plate 172 are heat-conducting plates. The upper plate 171 and the lower plate 172 are pressed together to form a reaction cavity 11 and a heat transfer plate 21 located outside the reaction cavity 11.

[0210] The reaction liquid carrier provided in this embodiment, by defining the specific structure of the first cavity structure 1, provides a pressing structure that enables rapid heat and cold conduction, which can significantly improve the temperature control efficiency of the heat source device and the cold source device on the reaction liquid in the reaction cavity 11 through the heat transfer plate 21 and the first cavity structure 1.

[0211] See also Figure 6 As shown, before pressing, the upper plate 171 and lower plate 172 do not form the heat transfer plate 21. (See reference for details.) Figure 5 As shown, the upper plate 171 and the lower plate 172 are pressed together to form the reaction chamber 11 and the heat transfer plate 21. Therefore, the first cavity structure 1 and the heat transfer plate 21 are integrally formed. In this type of structure, the heating or cooling of the heat transfer plate 21 can be quickly transferred to the inner wall of the first cavity structure 1 to control the temperature of the reaction liquid in the reaction chamber 11. At the same time, the integrally formed structure is conducive to the processing of small structures and the connection stability of small structures, avoiding the occurrence of unstable connection between the heat transfer plate 21 and the first cavity structure 1.

[0212] like Figure 6 , Figures 16 to 25 As shown, in one specific embodiment, the intermediate plate 22 is connected between the upper plate 171 and the lower plate 172. The upper plate 171, the intermediate plate 22 and the lower plate 172 are all made of thermally conductive materials. The intermediate plate 22, the upper plate 171 and the lower plate 172 are pressed together to form a reaction chamber 11. The intermediate plate 22 is located inside the reaction chamber 11.

[0213] The reaction liquid carrier provided in this embodiment, by defining the specific structure of the first cavity structure 1 and the intermediate plate 22, provides a pressing structure capable of rapid heat and cold conduction, which can significantly improve the temperature control efficiency of the reaction liquid in the temperature control cavity 111. In this embodiment, the upper plate 171, the intermediate plate 22, and the lower plate 172 are all structures made of thermally conductive materials. In a specific embodiment, the upper plate 171, the intermediate plate 22, and the lower plate 172 are all made of thermally conductive materials with a thermal conductivity of not less than 200 W / m·K, so that heat can be quickly transferred to the reaction cavity 11 or heat in the reaction cavity 11 can be quickly transferred to the outside.

[0214] See also Figure 6 As shown, in the laminated embodiment, the upper layer 171 and lower layer 172 do not form a temperature control cavity 111 before pressing. (See reference [reference needed]) Figure 5 As shown, the upper plate 171, lower plate 172, and intermediate plate 22 are pressed together to form the reaction chamber 11, the temperature control chamber 111, and the intermediate plate 22. Therefore, the first chamber structure 1 and the intermediate plate 22 are integrally formed. In this type of structure, the temperature change of the first chamber structure 1 can be quickly transmitted to the inner wall of the first chamber structure 1 and the intermediate plate 22. The inner wall of the first chamber structure 1 and the intermediate plate 22 can control the temperature of the reaction liquid in each temperature control chamber 111. In this embodiment, multiple temperature control chambers 111 are stacked vertically, and the intermediate plate 22 is inserted into the reaction chamber 11 horizontally or in a certain direction. In other embodiments, the number of intermediate plates 22 is not specifically limited. The upper plate 171, lower plate 172, and multiple intermediate plates 22 can be pressed together to form multiple temperature control chambers 111 and multiple intermediate plates 22 located inside the reaction chamber 11.

[0215] like Figure 3 and Figure 11 As shown, in one specific embodiment, the first cavity structure 1 has an upper membrane 174, a middle plate 173, and a lower membrane 175 stacked together. The middle plate 173 is a structure made of a thermally conductive material. The upper membrane 174 and the lower membrane 175 are made of a light-transmitting thermally insulating material that does not interfere with the detection results of the reaction liquid in the reaction cavity 11. The upper membrane 174, the middle plate 173, and the lower membrane 175 are pressed together to form the reaction cavity 11 and the intermediate plate 22 located inside the reaction cavity 11.

[0216] The reaction liquid carrier provided in this embodiment, by defining the specific structure of the first cavity structure 1 and the intermediate plate 22, provides an etching structure that enables rapid heat and cold conduction, which can significantly improve the temperature control efficiency of the reaction liquid in the temperature control cavity 111. Meanwhile, the upper membrane 174 and the lower membrane 175 adopt a transparent membrane structure, which can realize the formation of a light-transmitting inner wall 14, which is beneficial to improve the photometric efficiency of the reaction liquid carrier. In a preferred embodiment, the upper membrane 174 and the lower membrane 175 are preferably made of a material with a light transmittance greater than 50% and a thermal conductivity not higher than 0.2 W / m·K, which does not interfere with the detection results of the reaction liquid in the reaction chamber 11. In this embodiment, the detection result is also the photometric result. In other embodiments, the detection result can also be the detection result of other detection methods. In the following embodiments, unless otherwise specified, the detection result can refer to the photometric result. In another specific embodiment, the middle plate 173 is made of a thermally conductive material with a thermal conductivity not lower than 200 W / m·K. Of course, those skilled in the art will understand that, without considering power consumption limitations, the thermal conductivity of the material used to make the middle plate 173 can be further increased.

[0217] In the planar arrangement embodiment, refer to [reference needed]. Figure 3 and Figure 11 As shown, the upper membrane 174, the middle plate 173, and the lower membrane 175 are pressed together to form the reaction chamber 11 and the intermediate plate 22. Therefore, the first cavity structure 1 and the intermediate plate 22 are integrally formed. In this embodiment, the middle plate 173 is made of a thermally conductive material, so that heat can be quickly transferred to the reaction chamber 11 or heat in the reaction chamber 11 can be quickly transferred to the outside. In this type of structure, the temperature change of the first cavity structure 1 can be quickly transferred to the inner wall of the first cavity structure 1 and the intermediate plate 22. The inner wall of the first cavity structure 1 and the intermediate plate 22 can control the temperature of the reaction liquid in each temperature-controlled cavity 111. In this embodiment, refer to the... Figures 26 to 28 As shown, temperature control cavities 111 are spaced apart along the same plane, and intermediate plates 22 are inserted into the reaction chamber 11 in a horizontal or certain direction. The temperature control cavities 111 are formed by the intermediate plates 22 and the inner wall of the first cavity structure 1. The incident area 15 is the upward opening of the middle plate 173, and the photometric area 16 is the downward opening of the middle plate 173. The openings of the incident area 15 and the photometric area 16 expose multiple temperature control cavities 111. The upper film 174 is a light-transmitting inner wall 14 covering the opening of the incident area 15, and the lower film 175 is a light-transmitting inner wall 14 covering the opening of the photometric area 16. In other embodiments, the number of intermediate plates 22 is not specifically limited. In this embodiment, the middle plate 173 has multiple intermediate plates 22 located inside the reaction chamber 11.

[0218] like Figure 12 and Figure 25As shown, in one specific embodiment, the thickness of the upper plate 171 and the lower plate 172 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0219] The reaction liquid carrier provided in this embodiment, by limiting the thickness of the upper plate 171 and the lower plate 172, can avoid the structural rigidity of the reaction liquid carrier caused by the upper plate 171 and the lower plate 172 being too thin, and can also avoid the problem of the heat transfer plate 21 and the outer wall of the first cavity structure 1 having too large a heat capacity and the heat or cold energy transfer efficiency being too low caused by the upper plate 171 and the lower plate 172 being too thick.

[0220] like Figures 1 to 5 As shown, in one specific embodiment, the thickness of the heat transfer plate 21 is greater than or equal to 0.3 mm and less than or equal to 2 mm.

[0221] The reaction liquid carrier provided in this embodiment can avoid the problem of insufficient structural rigidity of the reaction liquid carrier caused by the heat transfer plate 21 being too thin by limiting the thickness of the heat transfer plate 21. It can also avoid the problem of excessive heat capacity of the heat transfer plate 21 and low heat or cold energy transfer efficiency caused by the heat transfer plate 21 being too thick, such as slow cooling speed and increased heating power consumption.

[0222] like Figure 5 , Figure 6 , Figures 16 to 25 As shown, in one specific embodiment, the thickness of the intermediate plate 22 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0223] The reaction liquid carrier provided in this embodiment can avoid insufficient structural rigidity of the reaction liquid carrier due to the intermediate plate 22 being too thin by limiting the thickness of the intermediate plate 22. It can also avoid problems such as excessive heat capacity of the heat transfer plate 21 and low heat or cold energy transfer efficiency due to the intermediate plate 22 being too thick, such as slow cooling speed and increased heating power consumption.

[0224] like Figures 27 to 28 As shown, in one specific embodiment, the width of the temperature control cavity 111 is set to be on the same order of magnitude as the width of the intermediate plate 22.

[0225] The reaction liquid carrier provided in this embodiment can avoid the problem of insufficient reaction liquid flow caused by the relatively narrow width of the temperature control cavity 111 and the intermediate plate 22 by limiting the relationship between the width of the temperature control cavity 111 and the width of the intermediate plate 22. It can also avoid the problem that the temperature control efficiency of the intermediate plate 22 on the reaction liquid is affected by the heat transfer efficiency between the liquids because the relatively wide width of the temperature control cavity 111 and the intermediate plate 22 is too wide.

[0226] Specifically, in this embodiment, the temperature control cavities 111 are spaced apart along the same plane, and the intermediate plate 22 is inserted into the reaction cavity 11 in a horizontal or certain direction. The temperature control cavities 111 are formed by the intermediate plate 22 and the inner wall of the first cavity structure 1. The width of the temperature control cavities 111 is the width of the temperature control cavities 111 along the arrangement direction of the multiple temperature control cavities 111. The width of the intermediate plate 22 is the width of the intermediate plate 22 along the arrangement direction of the multiple temperature control cavities 111. The width is of the same order of magnitude, which means that the units of the width are the same and the order of magnitude is the same.

[0227] like Figures 27 to 28 As shown, in one specific embodiment, the width of the temperature control cavity 111 and the width of the intermediate plate 22 are greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0228] The reaction liquid carrier provided in this embodiment, by limiting the specific numerical range of the width of the temperature control cavity 111 and the width of the intermediate plate 22, can avoid the problem of insufficient structural rigidity of the reaction liquid carrier due to the intermediate plate 22 being too thin, and can also avoid the problem of excessive heat capacity of the heat transfer plate 21 and low heat or cold energy transfer efficiency due to the intermediate plate 22 being too thick; it can also avoid the problem of insufficient fluidity of the reaction liquid due to the temperature control cavity 111 being too narrow, and can also avoid the problem of the temperature control efficiency of the intermediate plate 22 on the reaction liquid being affected by the heat transfer efficiency between liquids due to the temperature control cavity 111 being too wide.

[0229] like Figure 11 As shown, in one specific embodiment, the thickness of the middle layer 173 is greater than or equal to 0.1 mm and less than or equal to 1 mm.

[0230] The reaction liquid carrier provided in this embodiment, by limiting the specific numerical range of the thickness of the middle plate 173, can avoid the problem of insufficient structural rigidity of the reaction liquid carrier due to excessively thin middle plate 173, and can also avoid the problem of excessively large heat capacity and low heat or cold energy transfer efficiency of the middle plate 173 due to excessively thick middle plate 173; it also avoids the problem of the temperature control efficiency of the middle plate 173 on the reaction liquid being affected by the heat transfer efficiency between liquids due to excessively thick middle plate 173.

[0231] like Figure 6 , Figures 16 to 25 As shown, in one specific embodiment, the height of the temperature control cavity 111 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0232] By limiting the specific numerical range of the height of the temperature control cavity 111, the problem of insufficient fluidity of the reaction liquid caused by the excessively small height of the temperature control cavity 111 can be avoided, as can the problem of the temperature control efficiency of the reaction liquid being affected by the heat transfer efficiency between liquids caused by the excessively large height of the temperature control cavity 111 can also be avoided.

[0233] In one specific embodiment, temperature control cavities 111 are spaced apart along the same plane, and intermediate plates 22 are inserted into reaction chambers 11 in a horizontal or certain direction, with the height of the temperature control cavities 111 being perpendicular to the plane; in another specific embodiment, multiple temperature control cavities 111 are stacked vertically, and intermediate plates 22 are inserted into reaction chambers 11 in a horizontal or certain direction, with the height of the temperature control cavities 111 being the height in the stacking direction.

[0234] like Figure 15 , Figure 18 , Figure 19 and Figure 25 As shown, in one specific embodiment, the thickness of the support member 7 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0235] The reaction liquid carrier provided in this embodiment, by limiting the specific numerical range of the thickness of the support member 7, can avoid the problem of insufficient structural rigidity of the reaction liquid carrier due to the support member 7 being too thin, and can also avoid the problem of excessive heat capacity of the support member 7 and low heat or cold energy transfer efficiency due to the support member 7 being too thick; it also avoids the problem of excessive thickness of the temperature control cavity 111 due to the support member 7 being too thick, which would affect the temperature control efficiency of the reaction liquid by the heat transfer efficiency between liquids.

[0236] In a specific embodiment of the stacked configuration, multiple temperature control chambers 111 are stacked vertically, and intermediate plates 22 are inserted into the reaction chamber 11 in a horizontal or certain direction. Support members 7 are connected between two adjacent intermediate plates 22. The thickness of the support member 7 is the height of the temperature control chambers 111 in the stacked configuration direction, and the thickness of the support member 7 is also the height of the temperature control chambers 111.

[0237] like Figures 16 to 25 As shown, in one specific embodiment, the upper plate 171 and the lower plate 172 have a heat-conducting layer 1701. The lower surface of the heat-conducting layer 1701 of the upper plate 171 is provided with an isolation layer 1702, and the upper surface of the heat-conducting layer 1701 of the lower plate 172 is provided with an isolation layer 1702. The isolation layer 1702 is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber 11. The heat-conducting layer 1701 is preferably made of aluminum, and the isolation layer 1702 is preferably made of a plastic film or an aluminum oxide film.

[0238] The reaction liquid carrier provided in this embodiment can improve the heat transfer efficiency of the upper plate 171 and the lower plate 172 by setting a heat-conducting layer 1701. The heat-conducting layer 1701 is preferably made of aluminum material, which makes the heat transfer efficiency of the upper plate 171 or the lower plate 172 higher. The isolation layer 1702 is provided on the lower surface of the heat-conducting layer 1701 of the upper plate 171 and on the upper surface of the heat-conducting layer 1701 of the lower plate 172, which can also prevent the heat-conducting layer 1701 from reacting with the reaction liquid and affecting the measurement effect of qPCR.

[0239] Specifically, in this embodiment, the upper plate 171 and the lower plate 172 are pressed together to form a reaction chamber 11. That is, the surface of the upper plate 171 facing the reaction chamber 11 is provided with an isolation layer 1702, and the surface of the lower plate 172 facing the reaction chamber 11 is also provided with an isolation layer 1702. At the same time, the isolation layer 1702 on the facing surfaces of the upper plate 171 and the lower plate 172 can also facilitate the pressing and welding of the upper plate 171 and the lower plate 172.

[0240] like Figures 16 to 25 As shown, in one specific embodiment, the intermediate plate 22 has a heat-conducting layer 1701. The upper and lower surfaces of the heat-conducting layer 1701 of the intermediate plate 22 are provided with an isolation layer 1702. The isolation layer 1702 is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber 11. The heat-conducting layer 1701 is preferably made of aluminum, and the isolation layer 1702 is preferably made of plastic film or aluminum oxide film.

[0241] The reaction liquid carrier provided in this embodiment can improve the heat transfer efficiency of the intermediate plate 22 by setting a heat-conducting layer 1701. The upper and lower surfaces of the heat-conducting layer 1701 are provided with isolation layers 1702 to avoid the heat-conducting layer 1701 from reacting with the reaction liquid and affecting the measurement effect of qPCR.

[0242] In the first specific embodiment, the upper plate 171, the lower plate 172 and the intermediate plate are pressed together to form each temperature control cavity 111. That is, the two surfaces of the intermediate plate 22 facing each temperature control cavity 111 are provided with an isolation layer 1702. At the same time, the surfaces of the intermediate plate 22 facing the upper plate 171 and the intermediate plate 22 facing the lower plate 172 are provided with an isolation layer 1702, which can also facilitate the pressing and welding of the intermediate plate 22 with the upper plate 171 and the lower plate 172.

[0243] like Figure 11 As shown, in one specific embodiment, an isolation layer 1702 is formed on the surface of the middle layer plate 173. The thickness of the isolation layer 1702, the upper film 174, and the lower film 175 is less than or equal to 50 μm. The isolation layer 1702 is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber 11.

[0244] In the planar arrangement embodiment, the reaction liquid carrier provided in this embodiment, by providing an oxide film on the surface of the middle layer plate 173, can avoid biological reactions between the middle layer plate 173 and the reaction liquid, thus avoiding affecting the measurement effect of qPCR. At the same time, limiting the thickness of the oxide film, the upper membrane 174, and the lower membrane 175 can ensure the light transmittance of the upper membrane 174 and the lower membrane 175, and can also ensure that the oxide film does not affect the temperature control efficiency of the middle layer plate 173 on the reaction liquid in the temperature control chamber 111.

[0245] like Figure 11 As shown, in one specific embodiment, the middle layer 173 is made of aluminum, and the upper layer 174 and the lower layer 175 are formed of carbonate or polypropylene.

[0246] The reaction liquid carrier provided in this embodiment, by setting the middle plate 173 to be made of aluminum material, can improve the heat transfer efficiency of the middle plate 173. At the same time, aluminum material can be used as a roll material, and its easy bending characteristics facilitate the molding of the reaction liquid carrier. The surface of aluminum material can easily form a dense and thin alumina film, which can avoid the reaction between the reaction reagent and the structure of the reaction liquid carrier, thus avoiding affecting the structural stability of the reaction liquid carrier. It can also save other materials and further reduce the weight of the reaction liquid carrier. Restricting the materials of the upper film 174 and the lower film 175 to carbonate material or polypropylene material is beneficial to ensure the light transmittance of the upper film 174 and the lower film 175, and can also facilitate the pressing and welding of the middle plate 173 with the upper film 174 and the lower film 175. In a preferred embodiment, the isolation layer 1702 is preferably made of alumina film.

[0247] like Figure 3 , Figure 5 , Figure 6 , Figure 11 , Figures 27 to 29 As shown, in one specific embodiment, the reaction liquid carrier further includes:

[0248] The flow guiding structure 4 has a flow guiding channel 41 and an exhaust channel 42 that are arranged through it. The flow guiding channel 41 is connected to the liquid inlet 12, and the exhaust channel 42 is connected to the outlet 13 through the exhaust chamber 6.

[0249] The reaction liquid carrier provided in this embodiment, by setting the flow guiding structure 4, facilitates the filling of the reaction liquid into the reaction chamber 11 of the reaction liquid carrier, avoiding the leakage of the reaction liquid due to the small opening of the reaction liquid carrier.

[0250] Specifically, in this embodiment, please refer to the following: Figure 5 and Figure 29As shown, the flow guiding structure 4 is generally cylindrical. In other embodiments, the flow guiding structure 4 can also be other shapes, without specific limitations. In this embodiment, the flow guiding structure 4 is provided with a flow guiding channel 41 and an exhaust channel 42. The flow guiding channel 41 is a funnel-shaped structure that is wider at the top and narrower at the bottom. The lower end of the flow guiding channel 41 is connected to the liquid inlet 12, and the upper end of the flow guiding channel 41 is connected to the outside. The lower end of the exhaust channel 42 is connected to the outlet 13, and the upper end of the exhaust channel 42 is connected to the outside. In other embodiments, the lower end of the exhaust channel 42 can also be connected to the exhaust chamber 6, without specific limitations. In a specific embodiment of the stacked embodiment, refer to... Figure 6 As shown, the first cavity structure 1 may have an inlet 12 and an outlet 13, and the flow guiding structure 4 is correspondingly connected to the inlet 12 and the outlet 13; in a specific embodiment of the planar arrangement embodiment, refer to Figure 27 As shown, there are two flow guiding structures 4. The first cavity structure 1 may have an inlet 12 and an outlet 13 located at both ends of multiple temperature control cavities 111. The inlet channel of one flow guiding structure 4 is connected to the inlet 12, and the exhaust channel 42 of the other flow guiding structure 4 is connected to the outlet 13. In other embodiments, there are no restrictions on the number and corresponding connection method of the flow guiding structures 4 with the inlet 12 and outlet 13.

[0251] In one specific embodiment, there are multiple first cavity structures 1, which are stacked and arranged in layers, and the temperature control cavities 111 of each first cavity structure 1 are arranged to overlap along their stacking direction.

[0252] The reaction liquid carrier provided in this embodiment, by arranging multiple first cavity structures 1 in an overlapping manner, can reduce the size of the temperature control cavity 111 or simultaneously perform temperature control operations on a larger volume of reaction liquid, which is beneficial for providing faster temperature control efficiency or a more efficient QPRC process for the reaction liquid, and achieving better photometric efficiency.

[0253] In one specific embodiment, there are multiple first cavity structures 1, and the multiple temperature control cavities 111 within each first cavity structure 1 are arranged in a planar arrangement. The multiple first cavity structures 1 are stacked vertically and connected sequentially along their stacking direction. In this embodiment, the reaction liquid carrier has an inlet 12 and an outlet 13. During the filling process, the reaction liquid is first injected into the temperature control cavity 111 at any end of the first cavity structure 1 located at the upper end of the multiple first cavity structures 1, and then sequentially fills the multiple temperature control cavities 111 within the first cavity structure 1 located at the upper end of the multiple first cavity structures 1. Then, the reaction liquid fills each first cavity structure 1 located below the first cavity structure 1 located at the upper end of the multiple first cavity structures 1 from top to bottom by gravity. In other embodiments, the multiple first cavity structures 1 may also be non-connected, that is, each first cavity structure 1 is provided with an inlet 12 and an outlet 13, and each first cavity structure 1 is filled with reaction liquid through its corresponding inlet 12 and outlet 13.

[0254] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of this utility model. It should be understood that the above descriptions are merely specific embodiments of this utility model and are not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the scope of protection of this utility model.

Claims

1. A reaction liquid carrier, characterized in that, include: The first cavity structure has a reaction chamber formed inside it; A heat transfer structure is formed on the outside and / or inside the reaction chamber, and the heat transfer structure is connected to the first chamber structure.

2. The reaction liquid carrier according to claim 1, characterized in that, The heat transfer structure includes at least one heat transfer plate, which extends outward from the outside of the reaction chamber.

3. The reaction liquid carrier according to claim 2, characterized in that, There are two heat transfer plates, which are arranged on opposite sides of the first cavity structure.

4. The reaction liquid carrier according to claim 2, characterized in that, The heat transfer structure includes at least one intermediate plate, which is disposed inside the reaction chamber and divides the reaction chamber into at least two temperature control chambers.

5. The reaction liquid carrier according to claim 4, characterized in that, The multiple temperature control chambers are isolated from each other. The first chamber structure has multiple liquid inlets and multiple liquid outlets, and the multiple liquid inlets and multiple liquid outlets are respectively connected to each of the temperature control chambers.

6. The reaction liquid carrier according to claim 4, characterized in that, The multiple temperature control chambers are connected sequentially along their arrangement direction. The first chamber structure has at least one liquid inlet and at least one liquid outlet, and the at least one liquid inlet and at least one liquid outlet are respectively connected to the multiple temperature control chambers.

7. The reaction liquid carrier according to claim 6, characterized in that, The intermediate plate and the inner wall of the first cavity structure form a liquid passage, and two adjacent temperature control cavities are connected through the liquid passage.

8. The reaction liquid carrier according to claim 6, characterized in that, The intermediate plate has liquid passages, which are connected to two adjacent temperature control chambers.

9. The reaction liquid carrier according to claim 7 or 8, characterized in that, Multiple temperature-controlled chambers are connected end-to-end through the liquid-passing channel.

10. The reaction liquid carrier according to claim 9, characterized in that, Multiple temperature control cavities are stacked together. The liquid inlet is connected to the temperature control cavity located at the top of the reaction liquid carrier, and the outlet is connected to the temperature control cavity located at the bottom of the reaction liquid carrier. The first cavity structure also has an exhaust cavity, one end of which is connected to the outside, and the other end of which is connected to the outlet.

11. The reaction liquid carrier according to claim 9, characterized in that, Multiple temperature control chambers are spaced apart along the same plane. The liquid inlet is connected to the temperature control chamber located at one end of the multiple temperature control chambers, and the outlet is connected to the temperature control chamber located at the other end of the multiple temperature control chambers.

12. The reaction liquid carrier according to any one of claims 5, 7, or 8, characterized in that, Multiple temperature control chambers are spaced apart along the same plane, and there are multiple outlets, each of which is connected to one end of a temperature control chamber.

13. The reaction liquid carrier according to claim 12, characterized in that, The outlet is equipped with a one-way structure for gas passage only.

14. The reaction liquid carrier according to claim 4, characterized in that, The first cavity structure has a light-transmitting inner wall that exposes at least one of the temperature-controlled cavities, and the first cavity structure also has an incident area, the two ends of which correspond to the light-transmitting inner wall and the external arrangement of the first cavity structure, respectively.

15. The reaction liquid carrier according to claim 14, characterized in that, The first cavity structure also has a photometering area, with the two ends of the photometering area corresponding to the light-transmitting inner wall and the external arrangement of the first cavity structure, respectively.

16. The reaction liquid carrier according to claim 15, characterized in that, The first cavity structure has two light-transmitting inner walls arranged opposite to each other, and the photometric area and the incident area are respectively arranged corresponding to the two light-transmitting inner walls.

17. The reaction liquid carrier according to claim 14 or 16, characterized in that, The intermediate plate has a light-transmitting structure facing the two adjacent reaction chambers.

18. The reaction liquid carrier according to claim 17, characterized in that, There are multiple intermediate plates, and the light-transmitting structures of the multiple intermediate plates are spaced apart along the opening direction of the incident area.

19. The reaction liquid carrier according to claim 18, characterized in that, The surface of the intermediate plate is arranged perpendicular to the opening direction of the incident area.

20. The reaction liquid carrier according to claim 16, characterized in that, The first cavity structure also has a support member, which is connected between two adjacent intermediate plates, and the support member and the two adjacent intermediate plates divide to form the temperature control cavity.

21. The reaction liquid carrier according to claim 20, characterized in that, The light-transmitting inner wall is formed on the side wall of the support member, and the light metering area and the incident area are disposed facing at least one of the light-transmitting inner walls of the support member.

22. The reaction liquid carrier according to claim 10, characterized in that, The first cavity structure has an upper plate and a lower plate stacked together, the upper plate and the lower plate being pressed together to form the reaction cavity, and the heat transfer plate located outside the reaction cavity.

23. The reaction liquid carrier according to claim 22, characterized in that, The intermediate plate is connected between the upper plate and the lower plate. The intermediate plate, the upper plate and the lower plate are pressed together to form the reaction chamber. The intermediate plate is located inside the reaction chamber. The intermediate plate, the upper plate and the lower plate are all made of thermally conductive material.

24. The reaction liquid carrier according to claim 23, characterized in that, The thermal conductivity of the intermediate plate, the upper plate, and the lower plate is not less than 200 W / m·K.

25. The reaction liquid carrier according to claim 11, characterized in that, The first cavity structure has an upper membrane, a middle plate, and a lower membrane stacked together. The upper membrane, the middle plate, and the lower membrane are pressed together to form the reaction cavity and the intermediate plate located inside the reaction cavity. The middle plate is made of a thermally conductive material, and the upper membrane and the lower membrane are made of a light-transmitting thermally insulating material that does not interfere with the detection results of the reaction liquid in the reaction cavity.

26. The reaction liquid carrier according to claim 25, characterized in that, The thermal conductivity of the middle layer is not less than 200 W / m·K, and the thermal conductivity of the upper and lower layers is not higher than 0.2 W / m·K.

27. The reaction liquid carrier according to claim 23, characterized in that, The thickness of the upper plate and the lower plate is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

28. The reaction liquid carrier according to claim 22, characterized in that, The thickness of the heat transfer plate is greater than or equal to 0.3 mm and less than or equal to 2 mm.

29. The reaction liquid carrier according to claim 23, characterized in that, The thickness of the intermediate plate is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

30. The reaction liquid carrier according to claim 25, characterized in that, The width of the temperature control cavity is set to be on the same order of magnitude as the width of the intermediate plate.

31. The reaction liquid carrier according to claim 30, characterized in that, The width of the temperature control cavity is greater than or equal to 0.1 mm and less than or equal to 0.5 mm compared to the width of the intermediate plate.

32. The reaction liquid carrier according to claim 25, characterized in that, The thickness of the middle layer plate is greater than or equal to 0.1 mm and less than or equal to 1 mm.

33. The reaction liquid carrier according to claim 4, characterized in that, The height of the temperature control cavity is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

34. The reaction liquid carrier according to claim 20, characterized in that, The thickness of the support member is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

35. The reaction liquid carrier according to claim 23, characterized in that, The upper and lower plates have thermally conductive layers. The lower surface of the thermally conductive layer of the upper plate is provided with an isolation layer, and the upper surface of the thermally conductive layer of the lower plate is provided with the isolation layer. The isolation layer is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

36. The reaction liquid carrier according to claim 35, characterized in that, The thermally conductive layer is made of aluminum, and the insulating layer is made of a plastic film or an aluminum oxide film.

37. The reaction liquid carrier according to claim 23, characterized in that, The intermediate plate has a heat-conducting layer, and both the upper and lower surfaces of the heat-conducting layer of the intermediate plate are provided with an isolation layer. The isolation layer is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

38. The reaction liquid carrier according to claim 37, characterized in that, The thermally conductive layer is made of aluminum, and the insulating layer is made of a plastic film or an aluminum oxide film.

39. The reaction liquid carrier according to claim 25, characterized in that, An isolation layer is formed on the surface of the middle layer plate. The thickness of the isolation layer, the upper film, and the lower film is less than or equal to 50 μm. The isolation layer is made of a material that does not interfere with the detection results of the reaction liquid in the reaction chamber.

40. The reaction liquid carrier according to claim 39, characterized in that, The middle layer is made of aluminum, the insulating layer is made of aluminum oxide film, and the upper and lower films are formed of carbonate or polypropylene materials.

41. The reaction liquid carrier according to claim 10, characterized in that, The reaction liquid carrier further includes: A flow guiding structure has a flow guiding channel and an exhaust channel that pass through it. The flow guiding channel is connected to the liquid inlet, and the exhaust channel is connected to the outlet through the exhaust chamber.

42. The reaction liquid carrier according to claim 11, characterized in that, The first cavity structure is multiple, and the multiple first cavity structures are stacked, with each temperature control cavity of each first cavity structure overlapping along its stacking direction.

43. A reaction liquid carrier, characterized in that, include: The first cavity structure has a reaction chamber formed inside it; A heat transfer structure is formed on the outer and inner sides of the reaction chamber. The heat transfer structure is connected to the first chamber structure. The heat transfer structure includes at least one intermediate plate. At least one intermediate plate is disposed on the inner side of the reaction chamber and divides the reaction chamber into multiple temperature control chambers.

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