A portable mouse sperm freezing device

Abstract

This utility model relates to the field of cryopreservation equipment technology and discloses a portable mouse sperm cryopreservation device, including a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank, and a measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is provided on the top of the liquid nitrogen tank, and a hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod, and a second foam float is fixedly connected to the bottom of the telescopic rod. A placement tray is fixedly connected to the outside of the second foam float, and a pressure cap is slidably connected to the outside of the telescopic rod. This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of the measuring rod and the first foam float. The placement tray is connected to the hanging rod via the telescopic rod and is equipped with a pressure cap and limiting components to securely fix the cryopreservation tube.

Description

Technical Field

[0001] This utility model relates to the field of cryopreservation equipment technology, specifically a portable mouse sperm cryopreservation device. Background Technology

[0002] Mice are currently the most widely used laboratory animals. Due to the emergence of gene knockout and transgenic technologies, a large number of mouse disease models need to be preserved and strains reconstructed. However, the method of preserving live animals and passing them down from generation to generation not only consumes a lot of human, material and financial resources, but may also lead to the loss of models due to gene contamination and gene drift. Therefore, sperm cryopreservation is a preferred method for preserving animals.

[0003] Currently, after extracting sperm samples, medical and research personnel typically mix the sperm samples with cryoprotectants and place them in cryovials. However, suspending these cryovials in liquid nitrogen tanks presents a challenge. Existing liquid nitrogen tanks cannot guarantee the stability of sperm samples. In field experiments, moving the liquid nitrogen tank can cause bumps, which can lead to collisions or the cryovials falling into the liquid nitrogen, affecting subsequent experiments or reproductive success rates. Therefore, there is an urgent need for a portable mouse sperm cryopreservation device to solve these problems. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a portable mouse sperm cryopreservation device with advantages such as stable placement. It solves the problems of bumps caused by moving the liquid nitrogen tank, the easy collision of the cryopreservation tubes in the liquid nitrogen tank, or their falling into the liquid nitrogen, which affects subsequent experiments or breeding success rates.

[0006] (II) Technical Solution

[0007] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: A portable mouse sperm freezing device includes a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank. A measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is opened on the top of the liquid nitrogen tank. A hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod. A second foam float is fixedly connected to the bottom of the telescopic rod. A placement plate is fixedly connected to the outside of the second foam float. A pressure cap is slidably connected to the outside of the telescopic rod. A limit component is provided on the top of the pressure cap.

[0008] The beneficial effects of this utility model are:

[0009] This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of a measuring rod and a first foam float. The placement tray is connected to the hanging rod via a telescopic rod and is equipped with a pressure cap and limiting components to firmly fix the cryopreservation tubes, ensuring stable placement of sperm samples even during equipment movement or vibration.

[0010] Based on the above technical solution, the present invention can be further improved as follows.

[0011] Furthermore, the limiting assembly includes a limiting shell fixedly connected to the top of the pressure cap, a limiting spring fixedly connected inside the limiting shell, a limiting block fixedly connected outside the limiting spring, a limiting hole opened inside the telescopic rod, and the end of the limiting block away from the limiting spring being inserted into the limiting hole.

[0012] The beneficial effect of adopting the above-mentioned further solution is that by using the cooperation of the limiting component and the limiting hole, the pressure cap can keep the freezing tube in a fixed position, thereby improving stability.

[0013] Furthermore, the placement tray has placement holes arranged in a circular array inside, and freezing tubes are inserted into the placement holes.

[0014] The advantage of adopting the above-mentioned further solution is that the design of the circumferential array of placement holes inside the placement tray can neatly and orderly accommodate multiple freezing tubes.

[0015] Furthermore, an addition tube is fixedly connected to the top of the liquid nitrogen tank, and a first cap is fitted over the outside of the addition tube. The top of the measuring rod extends into the inside of the addition tube.

[0016] The advantage of adopting the above-mentioned further solution is that the addition tube at the top of the liquid nitrogen tank allows users to replenish liquid nitrogen at any time without frequently disassembling the cap or tilting the tank, making the operation more convenient and safer.

[0017] Furthermore, a second cover is fitted on the top of the liquid nitrogen tank, and a heat-insulating foam is fixedly connected to the bottom of the second cover. The heat-insulating foam is inserted into the interior of the liquid nitrogen tank, and a placement groove adapted to the hanging rod is opened inside the heat-insulating foam.

[0018] The beneficial effects of adopting the above-mentioned further solution are that the insulation foam at the bottom of the second cover is inserted into the liquid nitrogen tank, which can effectively reduce the evaporation of liquid nitrogen, improve the utilization rate of liquid nitrogen, extend the service life of the equipment, and reduce operating costs.

[0019] Furthermore, a handle is rotatably connected to the top of the liquid nitrogen tank, a first fixing member is fixedly connected to the outside of the second cover, and a second fixing member is fixedly connected to the outside of the liquid nitrogen tank.

[0020] The advantage of adopting the above-mentioned further solution is that the handle on the top of the liquid nitrogen tank makes it easier for users to carry and move the equipment, making the operation easier and more convenient. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of this utility model;

[0022] Figure 2 This is a schematic diagram of the thermal insulation foam in this utility model;

[0023] Figure 3 This is a cross-sectional view of the structure of this utility model;

[0024] Figure 4 This is a schematic diagram of the telescopic rod in this utility model;

[0025] Figure 5 This is a schematic diagram of the limiting shell in this utility model.

[0026] In the diagram: 1. Liquid nitrogen tank; 2. Positioning component; 3. Measuring rod; 4. First foam float; 5. Hanging rod; 6. Telescopic rod; 7. Second foam float; 8. Placement tray; 9. Pressure cap; 10. Limiting shell; 11. Limiting spring; 12. Limiting block; 13. Placement hole; 14. Freezing tube; 15. Adding tube; 16. First cap; 17. Second cap; 18. Insulating foam; 19. Handle; 20. First fixing component; 21. Second fixing component. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] In the embodiments, by Figure 1-5 The present invention discloses a portable mouse sperm cryopreservation device, comprising a liquid nitrogen tank 1, a hanging groove on the top of the liquid nitrogen tank 1, a hanging rod 5 hanging inside the hanging groove, a telescopic rod 6 slidably connected inside the hanging rod 5, a second foam float 7 fixedly connected to the bottom of the telescopic rod 6, a placement tray 8 fixedly connected to the outside of the second foam float 7, a pressure cap 9 slidably connected to the outside of the telescopic rod 6, and a placement hole 13 arranged in a circumferential array inside the placement tray 8, into which a cryopreservation tube 14 is inserted.

[0029] In this embodiment, the design of the circumferential array placement holes 13 inside the placement tray 8 can neatly and orderly accommodate multiple cryotubes, improving the utilization rate of the liquid nitrogen tank 1. It can simultaneously freeze and preserve a large number of mouse sperm samples to meet the needs of experiments of different scales. Moreover, this arrangement is conducive to the uniform distribution of liquid nitrogen, so that the sperm in each cryotube 14 can be in the same low temperature environment, ensuring the consistency of freezing effect and helping to improve the sperm survival rate and subsequent use quality.

[0030] Specifically, refer to Figure 5 The top of the pressure cap 9 is provided with a limiting component, which includes a limiting shell 10 fixedly connected to the top of the pressure cap 9. A limiting spring 11 is fixedly connected inside the limiting shell 10, and a limiting block 12 is fixedly connected outside the limiting spring 11. A limiting hole is opened inside the telescopic rod 6, and the end of the limiting block 12 away from the limiting spring 11 is inserted into the inside of the limiting hole.

[0031] In this embodiment, in actual use, the hanging rod 5 is provided with a limiting block to prevent the telescopic rod 6 from falling out. Both the hanging rod 5 and the telescopic rod 6 are square in shape to prevent the telescopic rod 6 from rotating and further improve stability. The limiting component and the limiting hole are used to keep the pressure cap 9 in a fixed position on the cryotube 14, thereby improving the reliability of sperm sample placement.

[0032] It should be noted that when the liquid nitrogen inside the liquid nitrogen tank 1 is continuously consumed, the second foam float 7 ensures that the freezing tube 14 is always suspended 3-5 cm above the liquid nitrogen surface.

[0033] Specifically, refer to Figure 3 The top of the liquid nitrogen tank 1 is fixedly connected to an adding tube 15. The outside of the adding tube 15 is covered with a first cap 16. The top of the liquid nitrogen tank 1 is covered with a second cap 17. The bottom of the second cap 17 is fixedly connected to a heat-insulating foam 18. The heat-insulating foam 18 is inserted into the inside of the liquid nitrogen tank 1. The inside of the heat-insulating foam 18 is provided with a placement groove that matches the hanging rod 5.

[0034] In this embodiment, in actual use, the insulating foam 18 at the bottom of the second cover 17 is inserted into the liquid nitrogen tank 1, which can effectively reduce the evaporation of liquid nitrogen, improve the utilization rate of liquid nitrogen, extend the service life of the equipment, and reduce operating costs. The placement groove inside the insulating foam 18 is compatible with the hanging rod 5, which not only provides stable support for the hanging rod 5, but also further enhances the overall structural stability of the equipment, prevents the hanging rod 5 from shaking and affecting the stability of the placement tray 8 and sperm sample, and also helps to maintain the low temperature environment inside the liquid nitrogen tank 1, reduce heat transfer, and ensure the continuous stability of the sperm freezing effect.

[0035] Specifically, refer to Figure 1A handle 19 is rotatably connected to the top of the liquid nitrogen tank 1; a first fixing member 20 is fixedly connected to the outside of the second cover 17; and a second fixing member 21 is fixedly connected to the outside of the liquid nitrogen tank 1.

[0036] In this embodiment, in actual use, the handle 19 on the top of the liquid nitrogen tank 1 makes it easy for users to carry and move the equipment, making operation easier and more convenient. The first fixing member 20 and the second fixing member 21 both have positioning holes. By passing bolts or locks through the positioning holes, the second cover 17 can be firmly fixed to the liquid nitrogen tank 1, preventing the cover from loosening or falling off due to equipment movement or external interference, ensuring the sealing of the liquid nitrogen tank 1, avoiding liquid nitrogen leakage, ensuring the safe use of the equipment, and also providing a reliable sealed environment for sperm freezing, preventing external pollution and temperature fluctuations from having an adverse effect on sperm.

[0037] In this embodiment, in actual use, the addition pipe 15 on the top of the liquid nitrogen tank 1 allows users to replenish liquid nitrogen at any time without frequently disassembling the second cap or tilting the tank, making the operation more convenient and safer. The small opening of the hydraulic addition pipe 15 reduces the waste of liquid nitrogen and potential dangers to operators during the replenishment process.

[0038] Specifically, refer to Figure 3 A positioning component 2 is fixedly connected to the inner wall of the liquid nitrogen tank 1. A measuring rod 3 is slidably connected inside the positioning component 2. A first foam float 4 is fixedly connected to the bottom of the measuring rod 3. The top of the measuring rod 3 extends into the inside of the adding tube 15.

[0039] In this embodiment, considering that a large amount of white mist will be generated when liquid nitrogen is poured, making it impossible for humans to observe the amount of liquid nitrogen added to the liquid nitrogen tank 1, a scale is provided on the outside of the measuring rod 3, and the top of the measuring rod 3 extends into the inside of the adding tube 15, so that the first foam float 4 will float when liquid nitrogen is added. By observing the part of the measuring rod 3 exposed at the top of the adding tube 15, the operator can observe the liquid level change in real time, accurately control the amount of liquid nitrogen added, avoid liquid nitrogen overflow or insufficient addition, and further optimize the performance of the equipment.

[0040] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0041] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0042] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable mouse sperm freezing apparatus comprising a liquid nitrogen tank (1), characterized in that: A positioning component (2) is fixedly connected to the inner wall of the liquid nitrogen tank (1). A measuring rod (3) is slidably connected inside the positioning component (2). A first foam float (4) is fixedly connected to the bottom of the measuring rod (3). A hanging groove is provided on the top of the liquid nitrogen tank (1). A hanging rod (5) is hung inside the hanging groove. A telescopic rod (6) is slidably connected inside the hanging rod (5). A second foam float (7) is fixedly connected to the bottom of the telescopic rod (6). A placement plate (8) is fixedly connected to the outside of the second foam float (7). A pressure cap (9) is slidably connected to the outside of the telescopic rod (6). A limit component is provided on the top of the pressure cap (9).

2. The portable mouse sperm freezing device according to claim 1, characterized in that: The limiting assembly includes a limiting shell (10) fixedly connected to the top of the pressure cap (9), a limiting spring (11) fixedly connected inside the limiting shell (10), a limiting block (12) fixedly connected outside the limiting spring (11), a limiting hole is opened inside the telescopic rod (6), and one end of the limiting block (12) away from the limiting spring (11) is inserted into the inside of the limiting hole.

3. The portable mouse sperm freezing device according to claim 1, characterized in that: The placement tray (8) has placement holes (13) arranged in a circular array inside, and a freezing tube (14) is inserted into the placement holes (13).

4. The portable mouse sperm freezing device according to claim 1, characterized in that: The top of the liquid nitrogen tank (1) is fixedly connected to an addition tube (15), and the outside of the addition tube (15) is covered with a first cap (16). The top of the measuring rod (3) extends into the inside of the addition tube (15).

5. A portable mouse sperm freezing device according to claim 1, characterized in that: The top of the liquid nitrogen tank (1) is fitted with a second cover (17), and the bottom of the second cover (17) is fixedly connected with a heat-insulating foam (18). The heat-insulating foam (18) is inserted into the interior of the liquid nitrogen tank (1), and the interior of the heat-insulating foam (18) is provided with a placement groove that is compatible with the hanging rod (5).

6. A portable mouse sperm freezing device according to claim 5, characterized in that: The top of the liquid nitrogen tank (1) is rotatably connected to a handle (19), the second cover (17) is fixedly connected to a first fastener (20), and the liquid nitrogen tank (1) is fixedly connected to a second fastener (21).

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