Device for pouring liquid to reduce buffering force in biomacromolecule technological process

Abstract

The technical problem to be solved by the utility model is to provide a device for reducing buffering force during liquid pouring in a biomacromolecule process, kinetic energy of the liquid poured by the device is dissipated step by step, smooth transition of liquid flow is realized, local shear effect is greatly reduced, and gas-liquid mixing is effectively reduced. The device comprises a flow guide pipe, the flow guide pipe comprises a first branch pipe and a second branch pipe, the second branch pipe is trumpet-shaped, a first spiral flow guide plate is arranged in the first branch pipe, a second spiral flow guide plate is arranged in the second branch pipe, and a first spiral flow guide groove is formed in the surface of the first spiral flow guide plate; a second spiral flow guide groove is formed in the surface of the second spiral flow guide plate, a dispersing device used for dispersing liquid is arranged at the lower end of the second branch pipe, the vertically-poured liquid flow can be converted into spiral motion through the first spiral flow guide groove and the second spiral flow guide groove, the flow speed is reduced, and gas-liquid mixing is reduced; the flow velocity is further reduced through section expansion, and concentrated impact is avoided through a dispersion device.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental technology, specifically to a device for reducing buffering force when pouring liquid during biological macromolecule processes. Background Technology

[0002] In critical processes such as biomolecule purification, buffer replacement, and column loading, it is necessary to pour high-viscosity or sensitive liquids (such as protein solutions) into containers. Traditional pouring methods, due to the high flow rate and impact force of the liquid, easily generate turbulence or severe shear force at the bottle mouth, leading to conformational damage, aggregation, or loss of activity of proteins, which seriously affects the accuracy of experimental results and sample utilization. Existing tools (such as ordinary funnels or straight-mouth pourers) lack buffer design, cannot effectively disperse liquid kinetic energy, and are difficult to adapt to the precise requirements of different liquid properties (such as viscosity and surface tension). Utility Model Content

[0003] The technical problem to be solved by this utility model is to provide a device for reducing the buffering force when pouring liquid in the process of biomacromolecule processing. The kinetic energy of the liquid is dissipated step by step, so as to achieve a smooth transition of liquid flow, greatly reduce the local shear effect, and effectively reduce gas-liquid mixing.

[0004] The technical solution adopted by this utility model to solve its technical problem is as follows: the device for reducing the buffering force when pouring liquid in the process of biomacromolecule processing includes a guide tube, and the guide tube includes a first branch tube and a second branch tube;

[0005] An annular support plate is provided on the upper end of the outer side wall of the first branch pipe, and multiple notches are provided on the edge of the annular support plate;

[0006] The lower end of the first branch pipe is located at the upper end of the second branch pipe. The second branch pipe is trumpet-shaped with its large diameter end located at the bottom. The diameter of the small diameter end of the second branch pipe is equal to the diameter of the first branch pipe.

[0007] The first branch pipe is provided with a first spiral guide plate, and the second branch pipe is provided with a second spiral guide plate that matches the shape of the second branch pipe. The lower end of the first spiral guide plate and the upper end of the second spiral guide plate are connected in the spiral direction.

[0008] The surface of the first spiral guide plate is provided with a first spiral guide groove, and the surface of the second spiral guide plate is provided with a second spiral guide groove and is seamlessly connected with the first spiral guide groove;

[0009] The lower end of the second branch pipe is provided with a dispersion device for dispersing the liquid.

[0010] Furthermore, a funnel-shaped liquid filling shell is provided at the upper end of the first branch pipe;

[0011] The lower end of the liquid filling shell is nested in the upper end of the inner cavity of the first branch pipe and is in contact with the upper end of the first spiral guide plate;

[0012] The bottom of the liquid filling housing is provided with a leakage hole that corresponds to the upper end of the first spiral flow guiding groove. The diameter of the leakage hole is less than or equal to the width of the first spiral flow guiding groove.

[0013] Furthermore, the dispersing device includes a cylindrical dispersing base, the diameter of which is equal to the outer diameter of the large diameter end of the second branch pipe, and the upper end of the dispersing base is located at the large diameter end of the second branch pipe.

[0014] The dispersion base is provided with multiple dispersion holes, all of which are interconnected with the inner cavity of the second branch pipe.

[0015] Furthermore, the dispersion base is made of ceramic material.

[0016] Furthermore, all of the aforementioned dispersion holes are hexagonal honeycomb holes with a diameter of 0.5 mm and a wall thickness of 0.1 mm.

[0017] Furthermore, soft silicone curtains are provided at the lower end of the sidewalls of the dispersion holes;

[0018] The soft silicone curtain has a thickness of 0.2 mm and a length of 30 mm.

[0019] Furthermore, the surface of the dispersion base and the inner wall surfaces of the multiple dispersion holes are coated with a hydrophilic coating.

[0020] Furthermore, the first branch pipe, the second branch pipe, the first spiral guide plate, and the second spiral guide plate are all made of medical-grade polycarbonate or polytetrafluoroethylene.

[0021] The beneficial effects of this utility model are as follows:

[0022] 1. By setting the first spiral guide plate, the second spiral guide plate, and the corresponding first spiral guide groove and second spiral guide groove, the vertically tilted liquid flow can be converted into spiral motion, reducing the flow rate and reducing gas-liquid mixing; in addition, since the second branch is funnel-shaped and the large-diameter end is located at the bottom, the flow rate is further reduced by cross-sectional expansion, effectively reducing damage to the biomolecular structure.

[0023] 2. The liquid flow is divided into many microflows by a dispersion device to avoid concentrated impact and further avoid damage to the structure of biological macromolecules. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the structure of the device for reducing buffering force when pouring liquids in biological experiments, as described in this utility model;

[0025] Figure 2 This is a schematic diagram of the liquid filling shell described in this utility model;

[0026] Figure 3 This is a schematic diagram of the structure of the dispersion base and soft silicone curtain combination described in this utility model;

[0027] Figure 4 This is a schematic diagram of the structure of the first spiral guide plate and the second spiral guide plate combination described in this utility model;

[0028] The markings in the diagram are as follows: 1. Guide pipe 101, 102. First branch pipe 102, 2. Second spiral guide plate 2, 3. First spiral guide groove 4, 5. Second spiral guide groove 5. Liquid filling shell 6. Leakage hole 7. Soft silicone curtain 8. Dispersion base 9. Dispersion hole 10. Annular support plate 11. Notch 12. Detailed Implementation

[0029] The specific embodiments of this utility model will be further described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0030] It should be noted that all directional indicator terms such as "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" in the embodiments of this application indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. They are only used to explain the relative positional relationship and movement of the components in a specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0031] In this application, unless otherwise expressly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0032] Furthermore, if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0033] like Figure 1-4 As shown, the device for reducing buffering force when pouring liquid during biomacromolecule processing includes a guide pipe 1, which includes a first branch pipe 101 and a second branch pipe 102.

[0034] An annular support plate 11 is provided on the upper end of the outer wall of the first branch pipe 101. The annular support plate 11 is used to support the entire device at the mouth of the experimental bottle. That is, the part below the annular support plate 11 is located inside the experimental bottle. The outer diameter of the annular support plate 11 is greater than or equal to the outer diameter of the mouth of the experimental bottle. Multiple notches 12 are provided on the edge of the annular support plate 11. The multiple notches 12 are evenly distributed along the circumferential direction of the annular support plate 11. The multiple notches 12 are used to communicate between the inside of the experimental bottle and the outside. When pouring liquid, the air in the experimental bottle is discharged from the multiple notches 12 to prevent the air from being discharged from the inside of the device and affecting the downward flow of liquid and the formation of turbulence.

[0035] The lower end of the first branch pipe 101 is located at the upper end of the second branch pipe 102. The diameter of the first branch pipe 101 is the same from top to bottom. The second branch pipe 102 is trumpet-shaped with its large diameter end located at the bottom. The diameter of the small diameter end of the second branch pipe 102 is equal to the diameter of the first branch pipe 101. Preferably, the second branch pipe 102 is trumpet-shaped and the inner diameter of its large diameter end is three times the inner diameter of the first branch pipe 101. The outer diameter of the large diameter end of the second branch pipe 102 is smaller than the inner diameter of the mouth of the experimental bottle, ensuring that the second branch pipe 102 can be smoothly inserted into the interior of the experimental bottle.

[0036] The first branch pipe 101 is provided with a first spiral guide plate 2, and the second branch pipe 102 is provided with a second spiral guide plate 3 that matches the shape of the second branch pipe 102. That is, the diameter of the second spiral guide plate 3 increases from top to bottom. The lower end of the first spiral guide plate 2 and the upper end of the second spiral guide plate 3 are connected in the spiral direction. That is, the spiral direction of the first spiral guide plate 2 and the second spiral guide plate 3 are the same, and the two form a complete variable diameter spiral structure.

[0037] The surface of the first spiral guide plate 2 is provided with a first spiral guide groove 4, and the surface of the second spiral guide plate 3 is provided with a second spiral guide groove 5 and is seamlessly connected with the first spiral guide groove 4. The spiral guide groove can convert the vertically tilted liquid flow into spiral motion, effectively reducing the flow rate and reducing gas-liquid mixing. Since the diameter of the second spiral guide groove 5 increases as it goes down, the liquid flow rate is further reduced by cross-sectional expansion.

[0038] The lower end of the second branch pipe 102 is provided with a dispersing device for dispersing the liquid. The dispersing device divides the liquid flow into many micro-flows to avoid concentrated impact.

[0039] like Figure 1 , Figure 2 As shown, in this embodiment, in order to facilitate the addition of the liquid required for the experiment into the guide tube 1, the upper end of the first branch tube 101 is provided with a funnel-shaped liquid addition shell 6. It should be noted that the lower end of the liquid addition shell 6 is a sealed structure.

[0040] The lower end of the liquid filling shell 6 is nested in the upper end of the inner cavity of the first branch pipe 101 and is in contact with the upper end of the first spiral guide plate 2;

[0041] The bottom of the liquid filling housing 6 is provided with a leakage hole 7, which corresponds to the upper end of the first spiral guiding groove 4. The diameter of the leakage hole 7 is less than or equal to the width of the first spiral guiding groove 4, preferably less than the width of the first spiral guiding groove 4. Due to the design of the liquid filling housing 6 being larger at the top and smaller at the bottom, it is convenient to pour liquid through the upper end of the liquid filling housing 6. The liquid then leaks down through the leakage hole 7 provided at the bottom of the liquid filling housing 6 to the first spiral guiding groove 4, and then flows spirally along the first spiral guiding groove 4, and then flows into the second spiral guiding groove 5, until it enters the dispersion device and is divided into many micro-flows.

[0042] like Figure 1 , Figure 3 As shown, in this embodiment, preferably, the dispersing device includes a cylindrical dispersing base 9, the diameter of which is equal to the outer diameter of the large diameter end of the second branch pipe 102, and the upper end of the dispersing base 9 is located at the large diameter end of the second branch pipe 102.

[0043] The dispersion base 9 is provided with a plurality of dispersion holes 10, all of which are interconnected with the inner cavity of the second branch pipe 102. The liquid flowing out of the second spiral guide groove 5 can be divided by the plurality of dispersion holes 10 and flow out through the plurality of dispersion holes 10 to avoid concentrated impact. The number of the plurality of dispersion holes 10 can be up to hundreds, dividing the liquid flow into hundreds of microflows.

[0044] In this embodiment, preferably, the dispersion base 9 is made of ceramic material, which is resistant to acid, alkali and organic solvent corrosion, has stable chemical composition and will not contaminate the experimental liquid.

[0045] In this embodiment, preferably, all of the plurality of dispersion holes 10 are hexagonal honeycomb holes with a diameter of 0.5 mm and a wall thickness of 0.1 mm.

[0046] like Figure 1 , Figure 2 As shown, in this embodiment, in order to further avoid direct impact on the liquid surface, a soft silicone curtain 8 is provided at the lower end of the side wall of each dispersion hole 10. It should be noted that only one soft silicone curtain 8 is provided on the side wall shared by each dispersion hole 10 and other adjacent dispersion holes 10, and there is no need to repeat the setting.

[0047] The soft silicone curtain 8 has a thickness of 0.2mm and a length of 30mm. The soft silicone curtain 8 guides the liquid to flow down slowly, effectively avoiding direct impact on the liquid surface.

[0048] In this embodiment, in order to reduce the adsorption of proteins by the dispersion base 9, the surface of the dispersion base 9 and the inner wall surface of the plurality of dispersion holes 10 are coated with a hydrophilic coating. The hydrophilic coating is preferably polyvinylpyrrolidone, which can effectively reduce protein adsorption.

[0049] In this embodiment, preferably, the first branch pipe 101, the second branch pipe 102, the first spiral guide plate 2, and the second spiral guide plate 3 are all made of medical-grade polycarbonate or polytetrafluoroethylene.

[0050] The above are merely preferred embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. Device for reducing the buffer force of a liquid to be poured in a process for biological macromolecules, comprising a flow guide (1), characterized in that: The guide pipe (1) includes a first branch pipe (101) and a second branch pipe (102); An annular support plate (11) is provided on the upper end of the outer side wall of the first branch pipe (101), and multiple notches (12) are provided at the edge of the annular support plate (11); The lower end of the first branch pipe (101) is located at the upper end of the second branch pipe (102). The second branch pipe (102) is trumpet-shaped with its large diameter end located at the bottom. The diameter of the small diameter end of the second branch pipe (102) is equal to the diameter of the first branch pipe (101). The first branch pipe (101) is provided with a first spiral guide plate (2), and the second branch pipe (102) is provided with a second spiral guide plate (3) that matches the shape of the second branch pipe (102). The lower end of the first spiral guide plate (2) and the upper end of the second spiral guide plate (3) are connected in the spiral direction. The surface of the first spiral guide plate (2) is provided with a first spiral guide groove (4), and the surface of the second spiral guide plate (3) is provided with a second spiral guide groove (5) and is seamlessly connected with the first spiral guide groove (4); The lower end of the second branch pipe (102) is provided with a dispersion device for dispersing the liquid.

2. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 1, characterized in that: The upper end of the first branch pipe (101) is provided with a funnel-shaped liquid filling shell (6); The lower end of the liquid filling shell (6) is nested in the upper end of the inner cavity of the first branch pipe (101) and is in contact with the upper end of the first spiral guide plate (2); The bottom of the liquid filling housing (6) is provided with a leakage hole (7) which corresponds to the upper end of the first spiral flow guide groove (4). The diameter of the leakage hole (7) is less than or equal to the width of the first spiral flow guide groove (4).

3. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 1, characterized in that: The dispersing device includes a cylindrical dispersing base (9); The diameter of the dispersion base (9) is equal to the outer diameter of the large diameter end of the second branch pipe (102), and the upper end of the dispersion base (9) is located at the large diameter end of the second branch pipe (102). The dispersion base (9) is provided with a plurality of dispersion holes (10), all of which are interconnected with the inner cavity of the second branch pipe (102).

4. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 3, characterized in that: The dispersion base (9) is made of ceramic material.

5. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 3, characterized in that: The plurality of the dispersion holes (10) are all hexagonal honeycomb holes with a diameter of 0.5 mm and a wall thickness of 0.1 mm.

6. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 3, characterized in that: The lower end of the sidewall of each dispersion hole (10) is provided with a soft silicone curtain (8); The soft silicone curtain (8) has a thickness of 0.2 mm and a length of 30 mm.

7. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 3, characterized in that: The surface of the dispersion base (9) and the inner wall surface of the multiple dispersion holes (10) are coated with a hydrophilic coating.

8. The device for reducing buffering force when pouring liquid during biomacromolecule processing according to claim 1, characterized in that: The first branch pipe (101), the second branch pipe (102), the first spiral guide plate (2), and the second spiral guide plate (3) are all made of medical-grade polycarbonate or polytetrafluoroethylene.

Images