A multi-stage filtration membrane module

Abstract

The utility model provides a kind of multistage filter membrane assembly, comprising: base, the base top is provided with multiple filter drums, each filter drum is fixedly communicated by connecting pipe, one filter drum top is fixedly communicated with water inlet pipe, one filter drum top is fixedly communicated with water outlet pipe, and multiple filter mechanisms are provided on filter drum;Multistage filter mechanism includes the sealing cover of screw connection in filter drum top, and filter cartridge is inserted in filter drum interior. Multiple filter drums are connected in series, different filter cartridges are provided in each filter drum, the solution is graded filtered, the filtering precision is high, and the filter drum can be replaced easily, the number of filter drum can be expanded according to needs, to further improve the precision of filtration, improve the purity of protease.

Description

Technical Field

[0001] This utility model relates to the field of protease purification technology, specifically to a multi-stage filtration membrane assembly. Background Technology

[0002] The production of protease requires purification and separation. In short, the extraction process involves first breaking down biological tissues, then taking the clear liquid and adding reagents, and finally pouring the liquid into the enzyme extraction equipment. The equipment filters out impurities to obtain a liquid with a high protease content.

[0003] Chinese patent application CN202120473008.5 discloses a protease purification device, relating to the field of enzymology. This protease purification device includes a support frame, a shell fixed to the support frame, and a first cylinder fixed to the shell. The first cylinder has a centrifugal filtration mechanism inside for centrifuging and filtering a solution containing protease. The shell has a centrifugal power mechanism connected to the centrifugal filtration mechanism. The shell also has a fine filtration mechanism. Furthermore, the shell has a liquid power mechanism that delivers the centrifuged and filtered liquid from the first cylinder to the fine filtration mechanism. The top of the first cylinder has a top cover with an inlet pipe connecting to the interior of the first cylinder, and the inlet pipe has a first valve. This device provides good filtration for the protease purification process, has an ingenious structure, and the filter screen is replaceable and easy to replace. However, it still has the following drawbacks:

[0004] Insufficient filtration precision resulted in insufficient purity of trypsin. Utility Model Content

[0005] The present invention aims to solve the problems mentioned in the background art by providing a multi-stage filtration membrane assembly.

[0006] The specific technical solution is as follows:

[0007] A multi-stage filtration membrane assembly includes: a base, a plurality of filter cartridges disposed on the top of the base, each filter cartridge being fixedly connected to the others via connecting pipes, an inlet pipe being fixedly connected to the top of one filter cartridge, an outlet pipe being fixedly connected to the top of another filter cartridge, and a multi-stage filtration mechanism being disposed on the filter cartridges; the multi-stage filtration mechanism includes a sealing cap threadedly connected to the top of the filter cartridges, and a filter element being inserted inside the filter cartridges.

[0008] The multi-stage filtration membrane assembly described above includes: a water inlet channel opened inside the filter element; a water inlet hole corresponding to the water inlet channel opened on the top of the sealing cover; and a water outlet hole opened on the top of the sealing cover.

[0009] In the above-mentioned multi-stage filtration membrane assembly, a sealing ring corresponding to the filter element is fixedly installed at the center of the bottom of the sealing cover, and the water inlet is located at the center of the top of the sealing ring.

[0010] In the above-mentioned multi-stage filtration membrane assembly, a limiting platform is fixedly connected to the bottom of the filter cartridge, and a sealing ring is fixedly connected to the top of the limiting platform.

[0011] In the above-mentioned multi-stage filtration membrane assembly, a one-way valve is fixedly installed on the water inlet pipe, and a pressurization pipe is fixedly connected to the water inlet pipe downstream of the one-way valve.

[0012] In the aforementioned multi-stage filtration membrane assembly, a pressure gauge is fixedly installed on the top of one of the sealing caps near the inlet pipe.

[0013] In the above-mentioned multi-stage filtration membrane assembly, the filter cartridge is configured as a pressure-resistant housing.

[0014] The multi-stage filtration membrane assembly described above, wherein: the filter elements in the plurality of filter cartridges are arranged in descending order of molecular weight cutoff or pore size, including: the first stage is an ultrafiltration membrane with a molecular weight cutoff of 30-50kDa, the second stage is a membrane with a molecular weight cutoff of 10-20kDa, and the third stage is a microfiltration membrane of 0.22μm.

[0015] In the above-mentioned multi-stage filtration membrane assembly, the connecting tube is a detachable quick-connect fitting, and the filter cartridge is provided with an interface that matches the quick-connect fitting.

[0016] In the aforementioned multi-stage filtration membrane assembly, the pressurization tube is equipped with an automatic regulating valve, and the pressure gauge is an electronic pressure sensor electrically connected to the automatic regulating valve, forming a closed-loop pressure control system.

[0017] This utility model has the following beneficial effects:

[0018] Multiple filter cartridges are connected in series, each containing a different filter element, to perform staged filtration of the solution. This method offers high filtration accuracy and allows for easy replacement of filter cartridges. The number of filter cartridges can be expanded as needed to further improve filtration accuracy and increase the purity of the protease. Attached Figure Description

[0019] Figure 1 A schematic diagram of the structure of a multi-stage filtration membrane assembly provided in an embodiment of this utility model;

[0020] Figure 2 A schematic diagram of the internal structure of a filter cartridge for a multi-stage filtration membrane assembly provided in an embodiment of this utility model;

[0021] Figure 3A schematic diagram of the bottom of the sealing cap in a multi-stage filtration membrane assembly provided in this embodiment of the present invention;

[0022] Figure 4 This is a schematic diagram of the bottom structure of a filter cartridge in a multi-stage filtration membrane assembly provided in an embodiment of the present invention.

[0023] In the attached image:

[0024] 100. Base; 110. Filter cartridge; 111. Inlet pipe; 112. Connecting pipe; 113. Outlet pipe; 210. Filter element; 211. Inlet channel; 212. Limiting platform; 213. Sealing ring; 220. Sealing cover; 221. Inlet hole; 222. Outlet hole; 223. Sealing ring; 231. Pressurization pipe; 232. Pressure gauge. Detailed Implementation

[0025] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0026] The accompanying drawings are for illustrative purposes only and are schematic diagrams, not actual images. They should not be construed as limiting the scope of this patent. To better illustrate the embodiments of this utility model, some components in the drawings may be omitted, enlarged, or reduced, and do not represent the actual dimensions of the product. It is understandable to those skilled in the art that some well-known structures and their descriptions may be omitted in the drawings.

[0027] In the accompanying drawings of this utility model, the same or similar reference numerals correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "left," "right," "inner," and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms used to describe positional relationships in the drawings are only for illustrative purposes and should not be construed as limiting this patent. For those skilled in the art, the specific meaning of the above terms can be understood according to the specific circumstances.

[0028] In the description of this utility model, unless otherwise explicitly specified and limited, the term "connection" or similar designation indicating the connection relationship between components should be interpreted broadly. For example, it can refer to a fixed connection, a detachable connection, or an integral part; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0029] Example

[0030] This embodiment provides a multi-stage filtration membrane assembly for trypsin purification, such as... Figures 1-4 As shown, it includes: a base 100, a plurality of filter cylinders 110 are provided on the top of the base 100, each filter cylinder 110 is fixedly connected to each other by a connecting pipe 112, an inlet pipe 111 is fixedly connected to the top of one filter cylinder 110, an outlet pipe 113 is fixedly connected to the top of one filter cylinder 110, and a multi-stage filtration mechanism is provided on the filter cylinder 110; the multi-stage filtration mechanism includes a sealing cap 220 threadedly connected to the top of the filter cylinder 110, and a filter element 210 is inserted inside the filter cylinder 110.

[0031] A multi-stage filtration membrane assembly for purifying trypsin using the above technical solution is provided with multiple filter cartridges 110 connected in series. Each filter cartridge 110 is equipped with different filter elements to perform staged filtration of the solution. The filtration accuracy is high, and the filter cartridges 110 are easy to replace. The number of filter cartridges 110 can be expanded as needed to further improve the filtration accuracy and the purity of the protease.

[0032] To allow the solution to pass through the filter element 210 and be filtered, the multi-stage filtration mechanism also includes a water inlet channel 211 formed inside the filter element 210, a water inlet hole 221 corresponding to the water inlet channel 211 on the top of the sealing cover 220, and a water outlet hole 222 on the top of the sealing cover 220. The solution enters the filter cartridge 110 through the water inlet hole 221, flows through the water inlet channel 211, and is filtered by the filter element 210.

[0033] To improve the filtration effect of the solution and prevent the solution from being discharged from the outlet hole 222 without filtration, a sealing ring 213 corresponding to the filter element 210 is fixedly installed at the center of the bottom of the sealing cover 220, and the water inlet hole 221 is located at the center of the top of the sealing ring 213.

[0034] To allow the solution to flow from the filter element 210 into the filter cartridge 110, a limiting platform 212 is fixedly connected to the bottom of the filter cartridge 110, and a sealing ring 223 is fixedly connected to the top of the limiting platform 212.

[0035] To ensure unidirectional flow of the solution and prevent backflow, a one-way valve is fixedly installed on the inlet pipe 111. A pressurization pipe 231 is fixedly connected to the inlet pipe 111 downstream of the one-way valve. Air is introduced into the filter cartridge 110 through the pressurization pipe 231, thereby pressurizing the filter cartridge 110 and facilitating the filtration of the solution.

[0036] To ensure stable pressure inside the filter cartridge 110, a pressure gauge 232 is fixedly installed on the top of a sealing cap 220 near the water inlet pipe 111.

[0037] To prevent the filter cartridge 110 from being crushed, it is designed with a pressure-resistant shell. Inside the filter cartridge 110 are membranes with progressively increasing precision: an ultrafiltration membrane with an MWCO of 30-50 kDa to remove cell debris, large protein molecules, and other impurities, retaining trypsin at approximately 23.8 kDa; a membrane with an MWCO of 10-20 kDa to further separate smaller impurities such as extraneous proteins and peptides and concentrate the target enzyme; and a 0.22 μm microfiltration membrane to ensure the sterility of the final product. This multi-stage membrane separation achieves highly efficient purification of trypsin, combining impurity fractional removal with activity retention, making it easy to use.

[0038] The filter elements 210 within the multiple filter cartridges 110 are arranged in descending order of molecular weight cutoff or pore size, including: a first-stage ultrafiltration membrane with a molecular weight cutoff of 30-50 kDa, a second-stage membrane with a molecular weight cutoff of 10-20 kDa, and a third-stage microfiltration membrane of 0.22 μm. This staged filtration design sequentially removes large molecular impurities, small molecular protein contaminants, and peptides, achieving aseptic filtration and significantly improving the purity and activity retention rate of proteases.

[0039] The connecting pipe 112 is a detachable quick-connect fitting, and the filter cartridge 110 has an interface that matches the quick-connect fitting. This detachable connection design allows users to flexibly increase or decrease the number of filter cartridges to meet different filtration precision requirements, while simplifying maintenance and expansion operations.

[0040] The pressurization pipe 231 is equipped with an automatic regulating valve, and the pressure gauge 232 is an electronic pressure sensor electrically connected to the automatic regulating valve, forming a closed-loop pressure control system. This system enables real-time pressure monitoring and dynamic adjustment, ensuring stable pressure during filtration, preventing filter membrane damage or decreased filtration efficiency due to pressure fluctuations, and improving system reliability and automation.

[0041] In summary, the multi-stage filtration membrane assembly for trypsin purification provided in this embodiment has the following advantages: multiple filter cartridges 110 are connected in series, each filter cartridge 110 is equipped with different filter elements, the solution is filtered in stages, the filtration accuracy is high, and the filter cartridges 110 are easy to replace. The number of filter cartridges 110 can be expanded as needed to further improve the filtration accuracy and improve the purity of the protease.

[0042] In use, the solution is fed into the filter cartridge 110 through the inlet pipe 111. The solution is filtered through the filter element 210 inside the filter cartridge 110 in multiple stages. The filter cartridge 110 is pressurized through the pressurization pipe 231 to improve the filtration effect of the filter element 210. The pressure inside the filter cartridge 110 is observed through the pressure gauge 232 to keep the pressure stable, thereby keeping the filtration effect stable.

[0043] The above are merely preferred embodiments of the present utility model and are not intended to limit the implementation methods and protection scope of the present utility model. Those skilled in the art should realize that any equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-stage filtration membrane module, characterized in that, include: A base (100) is provided with a plurality of filter cylinders (110) on the top of the base (100). Each filter cylinder (110) is fixedly connected to the others by a connecting pipe (112). A water inlet pipe (111) is fixedly connected to the top of one filter cylinder (110), and a water outlet pipe (113) is fixedly connected to the top of one filter cylinder (110). A multi-stage filtration mechanism is provided on the filter cylinder (110). The multi-stage filtration mechanism includes a sealing cap (220) threaded to the top of the filter cylinder (110), and a filter element (210) is inserted inside the filter cylinder (110).

2. The multi-stage filtration membrane assembly according to claim 1, characterized in that, The multi-stage filtration mechanism also includes a water inlet channel (211) opened inside the filter element (210), and the top of the sealing cover (220) is provided with a water inlet hole (221) corresponding to the water inlet channel (211), and the top of the sealing cover (220) is provided with a water outlet hole (222).

3. The multi-stage filtration membrane assembly according to claim 2, characterized in that, The sealing cap (220) has a sealing ring (213) corresponding to the filter element (210) fixedly installed at the center of the bottom, and the water inlet (221) is located at the center of the top of the sealing ring (213).

4. The multi-stage filtration membrane assembly according to claim 3, characterized in that, The bottom of the filter cylinder (110) is fixedly connected to a limiting platform (212), and the top of the limiting platform (212) is fixedly connected to a sealing ring (223).

5. The multi-stage filtration membrane assembly according to claim 1, characterized in that, A one-way valve is fixedly installed on the water inlet pipe (111), and a pressurization pipe (231) is fixedly connected to the water inlet pipe (111) after the one-way valve.

6. The multi-stage filtration membrane assembly according to claim 5, characterized in that, A pressure gauge (232) is fixedly mounted on the top of one of the sealing caps (220) near the water inlet pipe (111).

7. The multi-stage filtration membrane assembly according to claim 1, characterized in that, The filter cartridge (110) is configured as a pressure-resistant housing.

8. The multi-stage filtration membrane assembly according to claim 1, characterized in that, The filter elements (210) in the multiple filter cartridges (110) are arranged in descending order of molecular weight cutoff or pore size, including: the first stage is an ultrafiltration membrane with a molecular weight cutoff of 30-50kDa, the second stage is a membrane with a molecular weight cutoff of 10-20kDa, and the third stage is a microfiltration membrane with a molecular weight cutoff of 0.22μm.

9. The multi-stage filtration membrane assembly according to claim 1, characterized in that, The connecting pipe (112) is a detachable quick-connect fitting, and the filter cylinder (110) is provided with an interface that matches the quick-connect fitting.

10. The multi-stage filtration membrane assembly according to claim 6, characterized in that, The pressurization pipe (231) is equipped with an automatic regulating valve, and the pressure gauge (232) is an electronic pressure sensor, which is electrically connected to the automatic regulating valve to form a closed-loop pressure control system.

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