A centrifugal device for umbilical cord blood stem cell separation

Abstract

The utility model discloses a centrifugal device is separated with umbilical cord blood stem cell, including assembly cylinder, the outer wall fixed connection of assembly cylinder has the fixed plate, the outer wall fixed connection of fixed plate has the support, the bottom fixed connection of support has the base plate, the bottom one side of assembly cylinder is installed motor. This centrifugal device is separated with umbilical cord blood stem cell, and the bearing positioning seat of the lower frame bottom installed with the chain wheel and the assembly cylinder bottom connection rotates and connects, thereby the chain wheel will rotate, and the docking shaft fixed through the locking pin is rotated, and the mounting pin head of installing in the docking shaft top is used for driving the support to rotate, and the aperture of opening in the support top is used for the placement of test tube, and the slot of opening in the outside of support top is used for the plug connection of test tube cover, and the test tube assembly hole of opening in the front end of test tube cover upper surface is used for the placement of test tube, under the rotation of support, the cell in test tube is centrifuged and handles.

Description

Technical Field

[0001] This utility model relates to the field of cell centrifugation, specifically a centrifugation device for separating umbilical cord blood stem cells. Background Technology

[0002] Umbilical cord blood, as an important source of stem cells, has received widespread attention in the fields of regenerative medicine and cell therapy in recent years. Umbilical cord blood contains abundant hematopoietic stem cells and mesenchymal stem cells, which possess the potential for self-renewal and multi-lineage differentiation, and can be used to treat various blood and immune diseases. Therefore, how to efficiently and safely isolate stem cells from umbilical cord blood has become a hot research topic.

[0003] Currently, commonly used methods for stem cell isolation include density gradient centrifugation, flow cytometry, and immunomagnetic bead separation. Among these, density gradient centrifugation is widely used due to its simplicity and low cost. However, existing centrifugation devices have some shortcomings in the process of umbilical cord blood stem cell isolation.

[0004] If the rotating components fail to achieve balanced braking, the centrifugal effect will be mediocre. Utility Model Content

[0005] The purpose of this invention is to provide a centrifuge device for separating umbilical cord blood stem cells, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A centrifuge device for separating umbilical cord blood stem cells includes an assembly cylinder, a fixing plate fixedly connected to the outer wall of the assembly cylinder, a support fixedly connected to the outer wall of the fixing plate, a base plate fixedly connected to the bottom of the support, a motor mounted on one side of the bottom of the assembly cylinder, a drive gear mounted on the drive end of the motor, a chain drivenly connected to the outer wall of the drive gear, and a chain wheel mounted on the end of the chain away from the drive gear, a lower frame fixedly connected to the bottom of the assembly cylinder, and a second triangular seat fixedly connected to the bottom of the lower frame, the bottom of the second triangular seat rotatably connected to the chain wheel, and a locking pin mounted on the bottom axis of the chain wheel.

[0008] As a further embodiment of this utility model: the top of the locking pin passes through the shaft of the chain wheel and is fixedly connected to a mating shaft, and the top of the mating shaft is fixedly connected to an installation pin.

[0009] As a further embodiment of this utility model: a triangular seat is rotatably connected to the upper part of the outer wall of the docking shaft, a bearing seat is installed in the middle part of the outer wall of the docking shaft, and a bearing seat is installed in the lower part of the outer wall of the docking shaft.

[0010] As a further embodiment of this utility model: an inner frame that penetrates the bottom of the assembly cylinder is installed in the inner cavity of the triangular seat, and the bearing seat is installed in the inner cavity of the inner frame.

[0011] As a further improvement of this utility model, an inner cylinder is installed in the inner cavity of the assembly cylinder.

[0012] As a further embodiment of this utility model: a bracket is installed between the top of the mounting pin and the docking shaft. A through groove is opened on the outer side of the upper surface of the bracket. A test tube sleeve is installed at the opening of the through groove. A test tube assembly hole is opened on the upper surface of the test tube sleeve. A test tube is installed at the opening of the test tube assembly hole. An opening is opened on the inner side of the upper surface of the bracket.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] This utility model controls the operation of a motor. A chain wheel connected to the motor drive end, in conjunction with a chain, drives the chain wheel to rotate. Simultaneously, the chain wheel is rotatably connected to a bearing positioning seat mounted on the bottom of the lower frame connected to the bottom of the assembly cylinder. This causes the chain wheel to rotate, driving the docking shaft, which is fixed by a locking pin, to rotate as well. The mounting pin head mounted on the top of the docking shaft is used to drive the support to rotate. A through-hole on the top of the support is used to place test tubes, and a through-groove on the outer side of the top of the support is used to insert test tube sleeves. The test tube assembly hole on the front end of the upper surface of the test tube sleeve is used to place the test tubes. With the rotation of the support, the cells inside the test tubes are centrifuged. Its structure is more optimized and its design is more reasonable. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of a centrifuge device for separating umbilical cord blood stem cells.

[0016] Figure 2 This is a partial structural diagram of a centrifuge device for separating umbilical cord blood stem cells.

[0017] Figure 3 This is a cross-sectional view of a centrifuge apparatus for separating umbilical cord blood stem cells.

[0018] Figure 4 This is a structural diagram of a scaffold in a centrifuge device for separating umbilical cord blood stem cells.

[0019] In the diagram: Assembly cylinder 1, bracket 2, centrifuge disc 3, base plate 4, motor 5, fixing plate 6, inner cylinder 7, mounting pin head 8, triangular seat one 9, docking shaft 10, bearing seat one 11, locking pin 12, chain wheel 13, bearing seat two 14, triangular seat two 15, docking plate 16, inner frame 17, bearing positioning seat 18, test tube sleeve 19, test tube 20, through groove 21, through opening 22, test tube assembly hole 23. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figures 1-4 In this embodiment of the present invention, a centrifuge device for separating umbilical cord blood stem cells includes an assembly cylinder 1. A fixing plate 6 is fixedly connected to the outer wall of the assembly cylinder 1. A support 2 is fixedly connected to the outer wall of the fixing plate 6. A base plate 4 is fixedly connected to the bottom of the support 2. A motor 5 is installed on one side of the bottom of the assembly cylinder 1. An active gear is installed at the drive end of the motor 5. A chain is drivenly connected to the outer wall of the active gear. A chain wheel 13 is installed at the end of the chain away from the active gear. A lower frame is fixedly connected to the bottom of the assembly cylinder 1. A triangular seat 2 15 is fixedly connected to the bottom of the lower frame. The bottom of the triangular seat 2 15 is rotatably connected to the chain wheel 13. A locking pin 12 is installed at the bottom axis of the chain wheel 13.

[0022] The top of the locking pin 12 passes through the shaft of the chain wheel 13 and is fixedly connected to the mating shaft 10. The top of the mating shaft 10 is fixedly connected to the mounting pin 8.

[0023] A triangular seat 9 is rotatably connected to the upper part of the outer wall of the docking shaft 10, a bearing seat 11 is installed in the middle part of the outer wall of the docking shaft 10, and a bearing seat 14 is installed in the lower part of the outer wall of the docking shaft 10.

[0024] An inner frame 17, which penetrates the bottom of the assembly cylinder 1, is installed in the inner cavity of the triangular seat 9, and the bearing seat 11 is installed in the inner cavity of the inner frame 17.

[0025] An inner cylinder 7 is installed in the inner cavity of the assembly cylinder 1.

[0026] A bracket 2 is installed between the top of the mounting pin 8 and the docking shaft 10. A through groove 21 is opened on the outer side of the upper surface of the bracket 2. A test tube sleeve 19 is installed at the opening of the through groove 21. A test tube assembly hole 23 is opened on the upper surface of the test tube sleeve 19. A test tube 20 is installed at the opening of the test tube assembly hole 23. A through opening 22 is opened on the inner side of the upper surface of the bracket 2.

[0027] The working principle of this utility model is as follows:

[0028] When in use, the control motor 5 operates, and the chain wheel connected to the drive end of the motor 5 cooperates with the chain to drive the chain wheel 13 to rotate. At the same time, the chain wheel 13 is rotatably connected to the bearing positioning seat 18 installed at the bottom of the lower frame connected to the bottom of the assembly cylinder 1, so that the chain wheel 13 will rotate and drive the docking shaft 10 fixed by the locking pin 12 to rotate. The mounting pin head 8 installed on the top of the docking shaft 10 is used to drive the bracket 2 to rotate.

[0029] The opening 22 at the top of the support 2 is used to place the test tube;

[0030] The slot 21 on the outer side of the top of the support 2 is used for the insertion of the test tube sleeve 19, and the test tube assembly hole 23 on the front end of the upper surface of the test tube sleeve 19 is used for the placement of the test tube 20. Under the rotation of the support 2, the cells in the test tube 20 are centrifuged.

[0031] The mating plate 16, mounted on the platform of the triangular seat 15, works in conjunction with the inner frame 17 to assemble the bearing seat 11, thereby achieving stable rotation of the mating shaft 10 through the bearing seat 11.

[0032] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A centrifuge device for separating umbilical cord blood stem cells, comprising an assembly cylinder (1), characterized in that: A fixing plate (6) is fixedly connected to the outer wall of the assembly cylinder (1). A bracket (2) is fixedly connected to the outer wall of the fixing plate (6). A base plate (4) is fixedly connected to the bottom of the bracket (2). A motor (5) is installed on one side of the bottom of the assembly cylinder (1). A drive gear is installed on the drive end of the motor (5). A chain is connected to the outer wall of the drive gear. A chain wheel (13) is installed at the end of the chain away from the drive gear. A lower frame is fixedly connected to the bottom of the assembly cylinder (1). A triangular seat (15) is fixedly connected to the bottom of the lower frame. The bottom of the triangular seat (15) is rotatably connected to the chain wheel (13). A locking pin (12) is installed at the bottom axis of the chain wheel (13).

2. The centrifuge device for separating umbilical cord blood stem cells according to claim 1, characterized in that: The top of the locking pin (12) passes through the shaft of the chain wheel (13) and is fixedly connected to the docking shaft (10), and the top of the docking shaft (10) is fixedly connected to the mounting pin (8).

3. The centrifuge device for separating umbilical cord blood stem cells according to claim 2, characterized in that: A triangular seat 1 (9) is rotatably connected to the upper part of the outer wall of the docking shaft (10), a bearing seat 1 (11) is installed in the middle part of the outer wall of the docking shaft (10), and a bearing seat 2 (14) is installed in the lower part of the outer wall of the docking shaft (10).

4. The centrifuge device for separating umbilical cord blood stem cells according to claim 3, characterized in that: The inner frame (17) that penetrates the bottom of the assembly cylinder (1) is installed in the inner cavity of the triangular seat (9), and the bearing seat (11) is installed in the inner cavity of the inner frame (17).

5. The centrifuge device for separating umbilical cord blood stem cells according to claim 1, characterized in that: The inner cavity of the assembly cylinder (1) is fitted with an inner cylinder (7).

6. The centrifuge device for separating umbilical cord blood stem cells according to claim 2, characterized in that: A bracket (2) is installed between the top of the mounting pin (8) and the docking shaft (10). A through groove (21) is provided on the outer side of the upper surface of the bracket (2). A test tube sleeve (19) is installed at the groove opening of the through groove (21). A test tube assembly hole (23) is provided on the upper surface of the test tube sleeve (19). A test tube (20) is installed at the groove opening of the test tube assembly hole (23). A through opening (22) is provided on the inner side of the upper surface of the bracket (2).

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