Microbial inoculant activation culture system

Abstract

The utility model belongs to the field of microorganism activation culture, provide a kind of microorganism strain activation culture system, including bacteria liquid tank, mixing tank, culture solution tank and multifunctional main shaft;Bacteria liquid tank is connected with mixing tank by first connecting pipe, culture solution tank is connected with mixing tank by second connecting pipe, mixing tank is used to mix the bacteria liquid and culture solution of bacteria liquid tank and culture solution tank input;Multifunctional main shaft is hollow structure, its bottom is located in mixing tank, multifunctional main shaft top is connected for driving the motor of its rotation, multifunctional main shaft top is connected with the first end of three-way valve, the second end and third end of three-way valve are connected with inlet pipe and suction pipe respectively, inlet pipe is connected with gas source, suction pipe is connected with suction pump;The utility model integrates three functions of aeration, stirring and liquid taking on multifunctional main shaft;Realize multifunctional cooperation by the mechanical structure design of shaft body, improve the integration level and work efficiency of system, realize the integrated operation of microorganism strain activation culture.

Description

Technical Field

[0001] This utility model belongs to the field of microbial activation and culture, and specifically relates to a microbial strain activation and culture system. Background Technology

[0002] In microbiological research, activation culture is often required to enhance the activity, reproductive capacity, and metabolic function of microbial strains. The key to this process lies in creating a suitable environment for microbial growth, providing necessary nutrients, and controlling culture conditions such as temperature, pH, and dissolved oxygen. Traditional microbial strain activation culture typically uses culture tanks, whose core functions rely on the coordinated operation of independent components such as aeration, stirring, and sampling. However, such systems exhibit significant technical bottlenecks in practical applications: First, there is a contradiction between structural complexity and space efficiency. The aeration (e.g., bottom aerators), stirring (e.g., paddle mixers), and liquid sampling (e.g., side-pipe sampling valves) modules of traditional culture tanks require independently configured drive devices, piping systems, and control systems. This necessitates multiple interfaces on the tank, increasing the number of mechanical seals and occupying significant space due to the dispersed component layout. Second, maintaining a sterile environment is challenging. The multi-interface design introduces a higher risk of leakage, and mechanical seals are prone to wear and tear after prolonged use, leading to culture medium leakage or the intrusion of external microorganisms. Third, operational efficiency and automation are low. Traditional systems separate aeration, mixing, and sampling, making dynamic coordination difficult and requiring time-consuming and labor-intensive manual operation. Finally, there are limitations in maintenance costs and functional expansion. The dispersed layout of independent components leads to complex equipment disassembly and assembly, and different functional modules are difficult to quickly replace to adapt to diverse microbial strains. Utility Model Content

[0003] To solve the above-mentioned technical problems, this utility model provides a microbial strain activation and cultivation system to address the issues in the prior art. The technical solution adopted by this utility model is as follows:

[0004] A microbial strain activation and culture system includes a bacterial culture tank, a mixing tank, a culture medium tank, and a multifunctional spindle;

[0005] The bacterial culture tank is connected to the mixing tank via a first connecting pipe, and the culture medium tank is connected to the mixing tank via a second connecting pipe. The mixing tank is used to mix the bacterial culture and culture medium input from the bacterial culture tank and the culture medium tank.

[0006] The multi-functional spindle has a hollow structure, with its bottom set inside the mixing tank. The top of the multi-functional spindle is connected to a motor for driving its rotation. The top of the multi-functional spindle is connected to the first end of a three-way valve. The second and third ends of the three-way valve are respectively connected to an air inlet pipe and a suction pipe. The air inlet pipe is connected to an air source, and the suction pipe is connected to a suction pump.

[0007] Furthermore, the multifunctional spindle is provided with multiple through holes.

[0008] Furthermore, a stirring component is fixedly connected to the side of the multifunctional spindle.

[0009] Furthermore, an inner cylinder is fixedly installed inside the mixing tank, and an annular cavity is formed between the outer wall of the inner cylinder and the inner wall of the mixing tank. Heating water and a heating device are installed inside the annular cavity to realize the water bath heating function.

[0010] Furthermore, the first connecting pipe and the second connecting pipe are connected to the inner cylinder.

[0011] Furthermore, a frame is fixedly connected to the top of the mixing tank, the motor is mounted on the frame, and the multi-functional spindle is rotatably connected to the frame.

[0012] Furthermore, a first exhaust pipe is fixedly connected to the frame, a second exhaust pipe is fixedly connected to the top of the mixing tank, an elastic corrugated pipe is fixedly connected between the first exhaust pipe and the second exhaust pipe, and a valve is provided on the first exhaust pipe.

[0013] Furthermore, a base is fixedly connected to the top of the frame. The base has a hollow structure. The top of the multi-functional spindle is rotatably disposed within the hollow portion of the base. A branch pipe is fixedly connected to the top of the hollow portion of the base, and the branch pipe is connected to the first end of the three-way valve.

[0014] This invention has the following beneficial effects: It integrates aeration, stirring, and liquid collection into a multi-functional spindle. Through the mechanical structure design of the spindle, it achieves multi-functional synergy, improves the system's integration and working efficiency, realizes integrated operation of microbial strain activation and cultivation, reduces mechanical sealing points, improves sealing performance, and avoids the cumbersome operation of multiple devices in traditional cultivation systems. At the same time, due to the design of the multi-functional spindle, stirring, aeration, and liquid collection operations can be carried out continuously in one device, avoiding contamination and loss of microbial strains during the transfer process, and improving the purity and success rate of cultivation. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of a microbial strain activation and culture system according to this utility model. Detailed Implementation

[0016] The following will be based on the embodiments of this utility model. Figure 1 The technical solutions in the embodiments of this utility model are clearly and completely described. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0017] like Figure 1 A microbial strain activation and culture system includes a bacterial liquid tank 1, a mixing tank 2, a culture medium tank 3, and a multifunctional spindle 14;

[0018] The bacterial culture tank 1 is connected to the mixing tank 2 via a first connecting pipe 19, and the culture medium tank 3 is connected to the mixing tank 2 via a second connecting pipe 20. The mixing tank 2 is used to mix the bacterial culture and culture medium input from the bacterial culture tank 1 and the culture medium tank 3.

[0019] The multi-functional spindle 14 has a hollow structure, with its bottom set inside the mixing tank 2. The top of the multi-functional spindle 14 is connected to a motor 12 for driving its rotation. The top of the multi-functional spindle 14 is connected to the first end of a three-way valve 5. The second and third ends of the three-way valve 5 are respectively connected to an air inlet pipe 4 and a suction pipe 9. The air inlet pipe 4 is connected to an air source, and the suction pipe 9 is connected to a suction pump.

[0020] In this invention, the bacterial solution tank 1 and the culture medium tank 3 are located on opposite sides of the mixing tank 2. The bacterial solution tank 1 stores bacterial solution, such as a suspension of beneficial bacteria or specific microorganisms. The culture medium tank 3 stores culture medium containing nutrients required for the growth of microorganisms, such as water, inorganic salts, carbon sources, and sugars. When it is necessary to activate the microorganisms, the bacterial solution and culture medium are first introduced into the mixing tank 2 through the first connecting pipe 19 and the second connecting pipe 20, respectively. The first connecting pipe 19 and the second connecting pipe 20 are equipped with corresponding pumps. Then, the motor 12 is started, driving the multi-functional spindle 14 to rotate within the mixing tank 2. The rotation of the multi-functional spindle 14 not only mixes the bacterial solution and culture medium in the mixing tank 2 through the stirring component 15 fixed to its side, improving the mixing uniformity, but also allows for the flow of gas or liquid through its hollow structure. By operating the three-way valve 5, the air inlet pipe 4 or the suction pipe 9 can be selectively connected to the interior of the multi-functional spindle 14. The air source connected to the air inlet pipe 4 can be an oxygen cylinder or a mixture of oxygen and carbon dioxide. The suction pipe 9 is connected to the corresponding suction pump (the air source and suction pump are not shown in the figure).

[0021] When the air inlet pipe 4 is connected, the first and second ends of the three-way valve 5 are connected, allowing gas to be introduced into the multi-functional spindle 14. The gas is then evenly released into the mixing tank 2 through the through-hole 17 on the multi-functional spindle 14, providing the necessary oxygen for the microorganisms and creating an aeration function that aids in their growth and reproduction. This achieves the simultaneous function of uniform mixing and aeration, which helps to fully activate and cultivate the microorganisms in the mixing tank 2.

[0022] When the suction tube 9 is connected, the first and third ends of the three-way valve 5 are connected. The suction pump draws suction from the inside of the multifunctional spindle 14 through the suction tube 9 to form a negative pressure. This allows the liquid sample in the mixing tank 2 to be drawn through the through hole 17 for detection and analysis. After the microbial strain is activated and cultured, the mixed liquid in the mixing tank 2 can be drawn through the through hole 17 to transfer and extract the activated and cultured microbial strain.

[0023] This invention integrates aeration, stirring, and liquid collection functions onto a multi-functional spindle 14. The mechanical structure design of the spindle enables multi-functional synergy, improving system integration and efficiency. It achieves integrated operation for microbial strain activation and cultivation, reduces mechanical sealing points, improves sealing performance, and avoids the cumbersome operation of multiple devices in traditional cultivation systems. Furthermore, the design of the multi-functional spindle 14 allows for continuous stirring, aeration, and liquid collection within a single device, preventing contamination and loss of microbial strains during transfer and improving the purity and success rate of the cultivation.

[0024] Furthermore, the multifunctional spindle 14 is provided with a plurality of through holes 17.

[0025] Furthermore, a stirring component 15 is fixedly connected to the side of the multifunctional spindle 14. The stirring component 15 can be a square frame structure for convenient and uniform stirring.

[0026] Furthermore, an inner cylinder 16 is fixedly installed inside the mixing tank 2, and an annular cavity is formed between the outer wall of the inner cylinder 16 and the inner wall of the mixing tank 2. A heating water body 18 and a heating device are installed inside the annular cavity to realize the water bath heating function.

[0027] The heating water 18 and the heating device can heat the bacterial solution and culture medium in the mixing tank 2, creating a suitable temperature environment for the growth of microbial strains, ensuring that the growth temperature of the strains is maintained within a suitable range, which helps the strains to multiply rapidly and maintain their activity. The heating device can be a conventional heating element such as an electric heating wire or a heating plate.

[0028] Furthermore, the first connecting pipe 19 and the second connecting pipe 20 are connected to the inner cylinder 16.

[0029] Furthermore, the top of the mixing tank 2 is fixedly connected to the frame 13, the motor 12 is mounted on the frame 13, and the multi-functional spindle 14 is rotatably connected to the frame 13 through bearings.

[0030] Specifically, the motor 12 can be connected to the multi-functional spindle 14 via pulleys, gears, and other components to drive its rotation.

[0031] Furthermore, a first exhaust pipe 6 is fixedly connected to the frame 13, and a second exhaust pipe 8 is fixedly connected to the top of the mixing tank 2. An elastic corrugated pipe 7 is fixedly connected between the first exhaust pipe 6 and the second exhaust pipe 8. A valve is provided on the first exhaust pipe 6. The second exhaust pipe 8 communicates with the interior of the mixing tank 2.

[0032] The combined design of the elastic corrugated pipe 7, the first exhaust pipe 6, and the second exhaust pipe 8 in this invention not only achieves effective gas discharge but also regulates the pressure inside the mixing tank 2 through the expansion and contraction of the elastic corrugated pipe 7, preventing damage to the microorganisms or cultivation failure due to excessive internal pressure. The elastic corrugated pipe 7 can be made of rubber.

[0033] Furthermore, the top of the frame 13 is fixedly connected to the base 10, which is a hollow structure. The top of the multi-functional spindle 14 is rotatably disposed within the hollow part of the base 10. The top of the hollow part of the base 10 is fixedly connected to a branch pipe, which is connected to the first end of the three-way valve 5.

[0034] Specifically, the upper outer side of the multi-functional spindle 14 is fixedly connected to the annular part 11, and the bottom of the base 10 is provided with a sealing groove that matches the annular part 11. The annular part 11 is placed in the sealing groove and a corresponding sealing ring is provided to achieve the sealing function.

[0035] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Any modifications, alterations, alterations, or substitutions made by those skilled in the art to the technical solutions of the present utility model without departing from the spirit of the present utility model shall fall within the protection scope defined by the claims of the present utility model.

Claims

1. A microbial strain activation and cultivation system, characterized in that, It includes a bacterial culture tank (1), a mixing tank (2), a culture medium tank (3), and a multi-functional spindle (14). The bacterial culture tank (1) is connected to the mixing tank (2) through a first connecting pipe (19), and the culture medium tank (3) is connected to the mixing tank (2) through a second connecting pipe (20). The mixing tank (2) is used to mix the bacterial culture and culture medium input from the bacterial culture tank (1) and the culture medium tank (3). The multi-functional spindle (14) has a hollow structure, with its bottom set inside the mixing tank (2). The top of the multi-functional spindle (14) is connected to a motor (12) for driving its rotation. The top of the multi-functional spindle (14) is connected to the first end of a three-way valve (5). The second and third ends of the three-way valve (5) are connected to the air inlet pipe (4) and the suction pipe (9) respectively. The air inlet pipe (4) is connected to the air source, and the suction pipe (9) is connected to the suction pump.

2. The microbial strain activation and culture system according to claim 1, characterized in that, The multi-functional spindle (14) is provided with multiple through holes (17).

3. The microbial strain activation and culture system according to claim 1, characterized in that, The multifunctional spindle (14) is fixedly connected to the stirring component (15) on its side.

4. The microbial strain activation and culture system according to claim 1, characterized in that, The mixing tank (2) is fixedly provided with an inner cylinder (16). An annular cavity is formed between the outer wall of the inner cylinder (16) and the inner wall of the mixing tank (2). A heating water body (18) and a heating device are provided in the annular cavity to realize the water bath heating function.

5. The microbial strain activation and cultivation system according to claim 4, characterized in that, The first connecting pipe (19) and the second connecting pipe (20) are connected to the inner cylinder (16).

6. The microbial strain activation and cultivation system according to claim 1, characterized in that, The mixing tank (2) is fixedly connected to the top of the frame (13), the motor (12) is mounted on the frame (13), and the multi-functional spindle (14) is rotatably connected to the frame (13).

7. A microbial strain activation and culture system according to claim 6, characterized in that, The first exhaust pipe (6) is fixedly connected to the frame (13), the second exhaust pipe (8) is fixedly connected to the top of the mixing tank (2), the first exhaust pipe (6) and the second exhaust pipe (8) are fixedly connected to an elastic corrugated pipe (7), and a valve is provided on the first exhaust pipe (6).

8. A microbial strain activation and cultivation system according to claim 6, characterized in that, The top of the frame (13) is fixedly connected to the base (10), the base (10) is a hollow structure, the top of the multi-functional spindle (14) is rotatably set in the hollow part of the base (10), the top of the hollow part of the base (10) is fixedly connected to the branch pipe, and the branch pipe is connected to the first end of the three-way valve (5).

Images