Bacterial fermentation gas production and collection test tubes
By designing a transparent plastic tube and a detachable gas collection assembly, the problems of unstable gas collection and easy leakage in microbial culture tubes were solved, achieving stability and sealing, simplifying operation, reducing costs, and improving the reliability of gas collection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HENAN JUNRUI BIOENGINEERING CO LTD
- Filing Date
- 2025-06-23
- Publication Date
- 2026-05-29
AI Technical Summary
Existing microbial culture tubes and gas collection supports have problems such as being unstable, prone to leakage, difficult to assemble, requiring special tools, and making it difficult to observe the gas collection process.
The tube is made of transparent plastic and contains a detachable gas collection assembly, including a cover and a gas collection bracket, which are connected as one unit by a connecting rod. The cover is inserted into the tube. The gas collection bracket is designed as a triangular or semi-circular baffle to increase stability, and a sealing gasket is placed inside the cover to improve the sealing performance.
This technology achieves stability and sealing of the gas collection components, simplifies operation, reduces production costs, avoids secondary pollution, and improves the reliability and ease of observation of gas collection.
Smart Images
Figure CN224299220U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological culture technology, and in particular to bacterial fermentation gas production and collection test tubes. Background Technology
[0002] Microorganisms decomposing specific substances or producing gases using external energy during their growth is a common phenomenon in nature. Examples include the growth of coliform bacteria, yeast fermentation, biogas production, and photosynthesis in algae. These phenomena are widely applied in human production and daily life and are closely related to human well-being. In laboratories, these gases are typically collected by inverting a small test tube inside a microbial culture tube. However, this method has drawbacks, such as difficulty in venting air from the top of the test tube before fermentation and difficulty in observing gas collection when the culture medium is too turbid.
[0003] Chinese patent CN218710426U discloses a microbial culture tube, including a tube body and a gas collecting component. One end of the tube body is open, forming an opening. The bottom of the inner wall of the tube body has a positioning step and a positioning protrusion, with the positioning step located below the positioning protrusion. The gas collecting component includes a gas collecting part and a first and second abutting part extending vertically along the gas collecting part, abutting the gas collecting part between the positioning step and the positioning protrusion. The gas collecting part and the inner wall of the tube together define a gas collecting chamber, and a vent hole connecting the gas collecting chamber to the upper part of the tube body. The wall of the gas collecting chamber is transparent. Although there is a positioning step, the gas collecting component can only contact the positioning step at one point at the top and one point at the bottom, making it unstable and prone to tipping over or leakage at the contact point between the gas collecting component and the test tube.
[0004] Chinese patent CN221789333U discloses a gas collecting support for a test tube and the test tube itself. The gas collecting support includes: a gas collecting inclined plate extending upwards in a predetermined direction n; a top plate disposed on top of the inclined plate and extending in the predetermined direction n, the side of the top plate being a first arc surface adapted to the inner wall of the test tube; a gas collecting space formed between the first side of the inclined plate and the lower surface of the top plate; and a cavity on the second side of the inclined plate serving as a flow space; and an anti-detachment structure including an anti-detachment surface adapted to the inner wall of the test tube, comprising a second arc surface opposite to the first arc surface and third and fourth arc surfaces located horizontally on either side of the second arc surface, the areas of which are larger than the area of the side of the inclined plate. The gas collecting support uses a vertical plate as a clamp and a component to tighten the gas collecting assembly and the tube wall, which is difficult to manufacture. Even slight errors in the width of the vertical plate affect its stability and installability, resulting in poor practical application. The gas collection bracket in this patent only focuses on reinforcing the "arc plate" and making it less prone to damage, while neglecting the original intention of improving the gas collection effect.
[0005] Because the existing gas collection brackets are independent, if the gas collection brackets are inserted too deeply into the gas collection chamber, the fermentation area will be too small, and the reaction will be insensitive. At the same time, the gas collection brackets are inconvenient to assemble and require special tools for installation. Special equipment is needed for large-scale production. The gas collection brackets are also easy to be missed or placed upside down, resulting in defective products. Utility Model Content
[0006] This invention aims to solve the above-mentioned problems by proposing a test tube for bacterial fermentation gas production and collection.
[0007] The technical solution of this utility model is implemented as follows:
[0008] A bacterial fermentation gas production and collection test tube includes a transparent tube body. A gas collection assembly is detachably connected and installed inside the tube body. The gas collection assembly includes a cap nested at the tube body opening and a gas collection bracket inserted into the bottom of the tube body. The cap and the gas collection bracket are connected as one unit by a connecting rod.
[0009] Furthermore, at least one sealing gasket is installed on the inner wall of the cover that contacts the tube body, and the sealing gasket is an annular rubber ring with an opening.
[0010] Furthermore, the inner wall of the cover is formed with a groove for inserting the sealing gasket.
[0011] Furthermore, a fixing ring is formed on the inner wall of the tube to restrict the position of the gas collecting support.
[0012] Furthermore, the gas collecting bracket includes a partition extending from bottom to top in a preset direction a. A first arc plate and a second arc plate are symmetrically fixed on both sides of the partition. A cover plate extending in the preset direction a is fixed on the top of the partition. The side of the cover plate is an arc surface adapted to the inner wall of the pipe. The partition, the first arc plate, the second arc plate, and the cover plate together form a gas collecting space. The side of the first arc plate and the second arc plate away from the gas collecting space is connected as a whole by a third baffle. A flow space is formed between the third baffle and the partition. The outer wall of the third baffle is in contact with the inner wall of the pipe.
[0013] Furthermore, the cross-section of the third baffle is triangular or semi-circular.
[0014] Furthermore, the area of the first arc plate and the second arc plate facing the partition is greater than the side area of the two sides of the partition, and the lower ends of the first arc plate, the second arc plate and the third baffle extend beyond the first arc plate to connect the gas collection space and the flow space.
[0015] Furthermore, after the gas collecting bracket is inserted into the bottom of the tube, the lower end face of the fixing ring contacts the upper end face of the gas collecting bracket.
[0016] Furthermore, the tube body is made of transparent plastic, and the bottom of the tube body is a concave arc shape.
[0017] Furthermore, a chamfer is formed on the inner wall of the end of the cover facing the opening of the tube.
[0018] The beneficial effects of this utility model by adopting the above technical solution are as follows: the tube body is made of plastic and is a disposable product, avoiding contamination during secondary use due to incomplete washing; the third baffle of the gas collecting bracket has a triangular or semi-circular cross-section, has compressibility elasticity, and the gas collecting component is easy to insert and remove, and has relative stability; the cover and the gas collecting bracket are connected as one unit by a connecting rod, so that the cover and the gas collecting bracket move synchronously, and the gas collecting component will not be missed when filling culture medium; the cover can be easily opened by plugging in during the experiment, which is convenient to operate; the cover and the tube body adopt a plug-in method, which is simple to produce and assemble, and easy to open; a sealing gasket is set in the cover body to improve the sealing performance. Attached Figure Description
[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0020] Figure 1 This is the first sectional view of the present invention;
[0021] Figure 2 This is a second sectional view of the present invention;
[0022] Figure 3 This is a cross-sectional view of the tube body of this utility model;
[0023] Figure 4 This is a perspective view of the gas collection component of this utility model;
[0024] Figure 5 This is a cross-sectional view of the gas collection component of this utility model;
[0025] Figure 6 This is a first perspective view of the gas collecting bracket of this utility model;
[0026] Figure 7 This is a second perspective view of the gas collection bracket of this utility model.
[0027] The annotations in the attached figures are explained as follows:
[0028] 1. Pipe body; 11. Fixing ring; 2. Gas collection assembly; 21. Cover; 22. Connecting rod; 23. Gas collection bracket; 231. Partition plate; 232. First arc plate; 233. Third baffle plate; 234. Cover plate; 235. Second arc plate; 24. Sealing gasket; 25. Slot. Detailed Implementation
[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0030] like Figures 1-5 As shown, the bacterial fermentation gas production and collection test tube includes a transparent plastic tube body 1, which facilitates observation of gas production. It is also disposable, resulting in low production costs. After use, it can be directly discarded without cleaning, avoiding contamination during secondary use due to incomplete cleaning. A gas collection component 2 is detachably installed inside the tube body 1. The gas collection component 2 includes a cover 21 nested at the opening of the tube body 1 and a gas collection bracket 23 inserted into the bottom of the tube body 1. The cover 21 and the gas collection bracket 23 are connected as one unit by a connecting rod 22. The cover 1 and the tube body 1 are installed in a plug-in nested manner, making it easy to open the cover. The cover 21 can directly drive the gas collection bracket 23 to move together, facilitating the installation and removal of the gas collection bracket 23.
[0031] In this embodiment, at least one sealing gasket 24 is installed on the inner wall of the cover 21 that contacts the tube 1. The sealing gasket 24 is an annular rubber ring with an opening, which facilitates the installation of the sealing gasket 24. The sealing gasket 24 can improve the sealing performance at the connection between the cover 21 and the tube 1. The sealing gasket 24 can be installed on the inner wall of the cover 21 or on the stepped surface inside that contacts the opening of the tube 1.
[0032] In this embodiment, a groove 25 for embedding the sealing gasket 24 is formed on the inner wall of the cover 21. The groove 25 can improve the firmness of the sealing gasket 24 after installation. The position of the groove 25 matches the position of the sealing gasket 24. When the groove 25 is formed on the inner wall of the cover 21, the inner ring of the sealing gasket 24 is embedded in the groove 25 and contacts the outer wall of the tube 1 to achieve a seal. When the groove 25 is formed on the stepped surface at the top of the cover 21, the lower surface of the sealing gasket 24 contacts the opening of the tube 1 to achieve a seal.
[0033] In this embodiment, a fixing ring 11 is formed on the inner wall of the tube body 1 to restrict the position of the gas collecting bracket 23. When the bottom of the inner tube body 1 is a plane, the distance between the fixing ring 11 and the bottom of the inner tube body 1 is the same as the height of the gas collecting bracket 23. That is, after the gas collecting bracket 23 is inserted into the bottom of the inner tube body 1, the lower end face of the fixing ring 11 contacts the upper end face of the gas collecting bracket 23. The fixing ring 11 can limit the position of the gas collecting bracket 23.
[0034] In this embodiment, as Figure 6 , Figure 7 As shown, the gas collecting bracket 23 includes a partition 231 extending from bottom to top in a preset direction a. A first arc plate 232 and a second arc plate 235 are symmetrically fixed on both sides of the partition 231. The curvature of the first arc plate 232 and the second arc plate 235 matches the inner wall of the pipe body 1. The partition 231 has a certain angle with the longitudinal axis, dividing the upper ring of the gas collecting bracket 1 into two arc segments with a ratio of 1:3 and the lower ring of the gas collecting bracket 1 into two arc segments with a ratio of 1:2. A plate extending in the preset direction a is fixed at the top of the partition 231. The extended cover plate 234 has a side surface that is an arc surface adapted to the inner wall of the pipe body 1. The partition plate 231, the first arc plate 232, the second arc plate 235 and the cover plate 234 together form a gas collection space (gas collection chamber). The side of the first arc plate 232 and the second arc plate 235 away from the gas collection space is connected as one unit by the third baffle 233. The third baffle 233 and the partition plate 231 form a flow space that facilitates the entry and exit of liquid. The outer wall of the third baffle 233 is in contact with the inner wall surface of the pipe body 1.
[0035] In this embodiment, the third baffle 233 has a triangular cross-section, which has compressibility and elasticity, making it easy to insert and remove the gas collection component, and it also has relative stability.
[0036] In this embodiment, the area of the first arc plate 232 and the second arc plate 235 facing the partition 231 is larger than the side area of the partition 231, increasing the contact area with the inner wall of the tube 1. The lower ends of the first arc plate 232, the second arc plate 235 and the third baffle 233 extend beyond the first arc plate 232, thereby connecting the gas collection space and the flow space, which facilitates liquid flow.
[0037] In this embodiment, the bottom of the tube 1 is a concave arc shape, which can increase the fermentation space, making it easier to store more culture medium and collect more fermentation gas.
[0038] In this embodiment, the inner wall of the end of the cover 21 facing the opening of the tube 1 is formed with a chamfer, which makes it easier to insert the tube 1 into the cover 21.
[0039] The working principle of this invention is as follows: During use, the culture medium is injected into the tube body 1. Then, holding the cap 21, the gas collecting support 23 is inserted into the tube body 1 until it moves to the lower end of the fixing ring 11 and is locked in place. At this time, the sealing gasket 24 is compressed, with a compression greater than 0.2 mm. The gas production in the gas collecting space can be observed through the transparent wall of the tube body 1. The type of bacteria (micro-gas-producing, gas-producing, and non-gas-producing) can be determined based on the amount of gas collected and its presence. When the gas collecting space is filled with bubbles, it indicates that the microorganisms in the culture medium have begun to produce a gas collecting reaction. Tilting the tube body 1 to the left or right allows the gas in the gas collecting space to be discharged outwards through the flow space and collected in the tube body 1. When the test tube needs to be opened inside the clean bench for experiments, simply pull out the cap 21 to pull out the gas collecting support 23 along with it.
[0040] Components not described in detail in this article are existing technologies.
[0041] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A test tube for bacterial fermentation gas production and collection, characterized by: The tube (1) is made of transparent material. A gas collection assembly (2) is installed inside the tube (1) by a detachable connection. The gas collection assembly (2) includes a cover (21) nested at the opening of the tube (1) and a gas collection bracket (23) inserted into the bottom of the tube (1). The cover (21) and the gas collection bracket (23) are connected as one unit by a connecting rod (22).
2. The bacterial fermentation gas production and collection test tube according to claim 1, characterized in that: At least one sealing gasket (24) is installed on the inner wall of the cover (21) that contacts the tube (1). The sealing gasket (24) is an annular rubber ring with an opening.
3. The bacterial fermentation gas production and collection test tube according to claim 2, characterized in that: The inner wall of the cover (21) is formed with a groove (25) for inserting the sealing gasket (24).
4. The bacterial fermentation gas production and collection test tube according to claim 1, characterized in that: A fixing ring (11) is formed on the inner wall of the tube (1) to restrict the position of the gas collecting support (23).
5. The bacterial fermentation gas production and collection test tube according to claim 1, characterized in that: The gas collection bracket (23) includes a partition (231) extending from bottom to top in a preset direction a. A first arc plate (232) and a second arc plate (235) are symmetrically fixed on both sides of the partition (231). A cover plate (234) extending in the preset direction a is fixed on the top of the partition (231). The side of the cover plate (234) is an arc surface adapted to the inner wall of the pipe body (1). The partition (231), the first arc plate (232), the second arc plate (235) and the cover plate (234) together form a gas collection space. The side of the first arc plate (232) and the second arc plate (235) away from the gas collection space is connected as one unit by a third baffle (233). A flow space is formed between the third baffle (233) and the partition (231). The outer wall of the third baffle (233) is in contact with the inner wall of the pipe body (1).
6. The bacterial fermentation gas production and collection test tube according to claim 5, characterized in that: The cross-section of the third baffle (233) is triangular or semi-circular.
7. The bacterial fermentation gas production and collection test tube according to claim 5, characterized in that: The area of the first arc plate (232) and the second arc plate (235) facing the partition (231) is greater than the side area of the partition (231). The lower ends of the first arc plate (232), the second arc plate (235) and the third baffle (233) extend beyond the first arc plate (232) to connect the gas collection space and the flow space.