A nematode isolator

Abstract

The utility model belongs to the field of nematode extraction technology, concretely is a kind of nematode separator, funnel and filter separation pipeline, funnel is detachable in the upper end of filter separation pipeline, filter screen is equipped in filter separation pipeline, and filter screen is located below funnel, water outlet is equipped on the lateral wall of filter separation pipeline, and water outlet is located below filter screen, valve is equipped on water outlet, and the lower end of filter separation pipeline is closed.The utility model can filter solution containing nematode, and obtain pure nematode by multiple separation.

Description

Technical Field

[0001] This utility model belongs to the field of nematode extraction technology, specifically a nematode separator. Background Technology

[0002] Soil nematodes are mainly distributed in the soil and can also parasitize plant roots, causing harm to plant growth and development. They have wide applications in scientific research such as agriculture, plant pathology, gene function, and cell differentiation. They also have high research value in environmental indicators and biological control. Therefore, soil nematodes have been isolated and widely used.

[0003] The Bellman separation technique uses a separation device consisting of a funnel and a screen inside the funnel. A rubber tube is connected to the funnel outlet, and the opening and closing of the outlet is controlled by a stop clamp. The sample is soaked in water in the funnel for 8 to 10 hours. The nematodes contained within the sample migrate into the water and pass through the screen, settling in the rubber tube at the bottom of the funnel. Opening the stop clamp allows the nematodes to flow out of the rubber tube and be collected. However, this separation device can only perform one separation at a time, and the resulting nematodes often contain soil impurities. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model provides a nematode separator that can filter solutions containing nematodes and obtain pure nematodes through multiple separations.

[0005] The technical solution of this utility model includes a funnel and a filter separation pipe. The funnel is detachable at the upper end of the filter separation pipe. A filter screen is provided inside the filter separation pipe and is located below the funnel. A water outlet is provided on the side wall of the filter separation pipe and is located below the filter screen. A valve is provided on the water outlet. The lower end of the filter separation pipe is closed.

[0006] Furthermore, the outlet is located at one end near the funnel.

[0007] Furthermore, the filtration and separation pipeline includes a first pipeline and a second pipeline. The upper end of the first pipeline is inserted into the funnel, and the filter screen is located inside the first pipeline. The lower end of the first pipeline is detachably and sealed to the upper end of the second pipeline. The lower end of the second pipeline is closed, and the outlet is located at the end of the second pipeline near the funnel.

[0008] Furthermore, there are several second pipes, each of which is connected to the first pipe through a diversion structure.

[0009] Furthermore, the diversion structure includes a multi-way diverter and several water distribution pipes. One port of the multi-way diverter is connected to the first pipe, and the remaining ports are connected to one end of each of the several water distribution pipes. The other ends of the several water distribution pipes are connected to several second pipes.

[0010] Furthermore, a third pipe is installed between the second pipe and the branch pipe.

[0011] Furthermore, the end of the second pipe furthest from the first pipe is tapered.

[0012] Furthermore, the filter screen and the filter separation pipe are detachably connected.

[0013] Furthermore, the filter screen includes two layers of gauze and one layer of toilet paper, with the toilet paper layer located between the two layers of gauze, and the gauze layer extending out of the upper port of the filter separation pipe.

[0014] The technical solution provided by this utility model embodiment has the following advantages compared with the prior art:

[0015] The solution containing the sample is poured into a funnel, flowing through which into a filtration separation pipe. A filter screen filters the solution, leaving large particles of impurities on the screen, while nematodes and small particles remain at the bottom of the filtration separation pipe. After standing for 10 to 12 minutes, taking advantage of the fact that live nematodes settle much faster than small particles in water, the nematodes sink to the bottom of the filtration separation pipe first. The outlet is then opened to drain the liquid above it before being closed. The funnel is removed, the filter screen is removed, and water is added back into the filtration separation pipe to resuspend the nematodes and impurities for 10 to 12 minutes. Water is then added again, and this process is repeated 3-5 times to obtain pure nematodes. Compared to existing technologies, this method can filter solutions containing nematodes and obtain pure nematodes through multiple separations.

[0016] Other advantages, objectives and features of this invention will be partly apparent from the following description, and partly understood by those skilled in the art through study and practice of this invention. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of the nematode separator according to an embodiment of the present invention;

[0019] Figure 2 This is a diagram showing the fit between the filter screen and the first pipe of the nematode separator according to an embodiment of the present invention;

[0020] Figure 3This is a diagram showing the combination of the multi-channel diverter and the water distribution pipe of the nematode separator according to an embodiment of this utility model;

[0021] Figure 4 This is a diagram showing the connection between the second and third pipes of the nematode separator according to an embodiment of the present invention.

[0022] Figure label:

[0023] 1. Funnel; 2. First pipe; 3. Filter screen; 4. Second pipe; 5. Outlet; 6. Multi-way distributor; 7. Divider pipe; 8. Third pipe; 9. Valve. Detailed Implementation

[0024] The following describes a specific embodiment of the present invention in detail with reference to the accompanying drawings. However, it should be understood that the scope of protection of the present invention is not limited to the specific embodiment.

[0025] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the technical solution of this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0026] In the description of the embodiments of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0027] In the description of the embodiments of this utility model, the pipes are all sealed and detachable connections.

[0028] like Figures 1 to 4 As shown, this utility model provides a nematode separator, which is fixed on a bracket (not shown in the figure). It includes a funnel 1 and a filter separation pipe. The funnel 1 is detachable at the upper end of the filter separation pipe. A filter screen 3 is provided inside the filter separation pipe and is located below the funnel 1. A water outlet 5 is provided on the side wall of the filter separation pipe and is located below the filter screen 3. A valve 9 is provided on the water outlet 5. The lower end of the filter separation pipe is closed.

[0029] Specifically, the solution containing the sample is poured into a funnel, which then flows into a filtration separation pipe. A filter screen filters the solution, leaving large particles of impurities on the screen. Nematodes and small particles of impurities remain at the bottom of the filtration separation pipe with the liquid. After standing for 10 to 12 minutes, the nematodes sink to the bottom of the filtration separation pipe due to the fact that the settling speed of live nematodes in water is much greater than that of a single bacterial cell. The water outlet is then opened to drain the liquid above the outlet and closed. The funnel is removed, the filter screen inside the filtration separation pipe is removed, and water is added back into the filtration separation pipe to resuspend the nematodes and impurities for 10 to 12 minutes. Water is then added again, and this process is repeated 3 to 5 times to obtain pure nematodes. Compared with existing technologies, this method can filter solutions containing nematodes and obtain pure nematodes through multiple separations.

[0030] Furthermore, the outlet 5 is located at one end near the funnel 1.

[0031] Furthermore, the filtration and separation pipeline includes a first pipeline 2 and a second pipeline 4. The upper end of the first pipeline 2 is inserted into the funnel 1, the filter screen 3 is located inside the first pipeline 2, the lower end of the first pipeline 2 is detachably and sealed to the upper end of the second pipeline 4, the lower end of the second pipeline 4 is closed, and the outlet 5 is located at the end of the second pipeline 4 near the funnel 1.

[0032] Furthermore, the pore size of filter 3 is set differently depending on the nematodes being detected. The specific pore size can be set by those skilled in the art according to actual needs, and this utility model does not impose any restrictions on it.

[0033] For example: plant parasitic nematodes are 40 μm in size, and the pore size of filter 3 is 40 μm; free-living nematodes are 60 μm in size, and the pore size of filter 3 is 60 μm.

[0034] It should be noted that when it is necessary to separate nematodes from the soil, wrap the soil in two layers of gauze and one layer of toilet paper, flatten it and place it in funnel 1. Then add water to submerge the soil and let it stand for 18-24 hours. This allows the nematodes to pass through the gauze and toilet paper and fall into funnel 1. Filter screen 3 can filter out larger soil particles and other impurities that may accidentally fall into the device when placing the sample. This allows the nematodes and small impurities to pass through filter screen 3 and enter the second pipe 4.

[0035] Furthermore, when separating nematodes from the soil, the selected funnel 1 has a large diameter and a small height, which can shorten the time for the nematodes to be released from the soil and improve the separation efficiency.

[0036] Furthermore, it can also be used to isolate nematodes artificially cultured on NGM medium. Since the surface of the NGM plate is coated with OP50 bacterial solution, when a large number of pure nematodes need to be separated after the nematode culture is completed, OP50 bacterial blocks, individual bacterial cells, and agar blocks are often present. The nematode solution is poured into funnel 1, and filter screen 3 can filter out large bacterial blocks and agar blocks. Nematodes and individual bacterial cells pass through filter screen 3 and enter the second pipe 4.

[0037] Optionally, filter 3 consists of two layers of gauze sandwiched with a layer of toilet paper, which can filter out large impurities without affecting the passage of nematodes, and is also economical.

[0038] Furthermore, the gauze extends out from the upper port of the filter separation pipe.

[0039] Optionally, the height of funnel 1 is about 8 cm, and the length of the pipe at the outlet of funnel 1 is about 1 cm.

[0040] It is understood that the 8cm height of funnel 1 is set according to the actual application scenario to increase the volume of insect liquid and soil. Of course, the height of funnel 1 will vary depending on the actual scenario, and those skilled in the art can set it according to actual needs. This utility model does not impose any restrictions on this.

[0041] Optionally, the length of the first pipe 2 is about 15 cm, the inner diameter is between 2.8 cm and 3 cm, and the filter screen 3 is set inside the first pipe 2 at a distance of about 5 cm from the pipe opening.

[0042] It is understandable that the primary function of the first pipe 2 is to house the filter screen 3, and secondly, to guide the flow and provide a channel for the nematodes to settle by gravity. The 15cm length is set according to the actual scenario, and those skilled in the art can set it according to actual needs; this utility model does not impose any limitations on this. The inner diameter of 2.8cm to 3cm is to increase the capacity and facilitate the diversion below. The filter screen 3 is positioned at 5cm to increase the volume above the filter screen 3 and increase the pressure, preventing problems such as low water pressure above the filter screen 3 and slow filtration speed due to the filter screen 3 being positioned too far forward.

[0043] In the embodiments provided by this utility model, there are multiple second pipes 4, each of which is connected to the first pipe 2 through a diversion structure. The diversion structure includes a multi-way diverter 6 and several water distribution pipes 7. The multi-way diverter 6 is connected to the first pipe 2, one end of each of the several water distribution pipes 7 is connected to the first pipe 2 through the multi-way diverter 6, and the other end of each of the several water distribution pipes 7 is connected to one of the multiple second pipes 4.

[0044] It is understandable that multiple second channels 4 are set up to collect nematodes because a large number of nematodes can be separated at one time, but the number of nematodes used in the experiment is relatively small. Multiple second channels 4 can be used to collect the nematodes separately.

[0045] Specifically: After filtration by filter screen 3, nematodes and tiny impurities flow with the liquid to the diversion structure, where they flow to different water distribution pipes 7, and then to different second pipes 4, separating the nematodes in the first pipe 2 for separate collection.

[0046] In the embodiments provided by this utility model, the inner diameter of each water distribution pipe 7 is smaller than the inner diameter of the first pipe 2.

[0047] In the embodiments provided by this utility model, the angle between each water distribution pipe 7 and the first pipe 2 is between 30 degrees and 60 degrees.

[0048] In the embodiments provided by this utility model, a third pipe 8 is provided between each second pipe 4 and the water distribution pipe 7.

[0049] It is understandable that the third pipe 8 is designed to increase the vertical suspension height, extend the settling time of bacteria and other microorganisms, and facilitate the settling and separation of nematodes.

[0050] In the embodiments provided by this utility model, the end of the second pipe 4 away from the first pipe 2 is tapered, i.e., a pointed bottom structure, so as to facilitate the collection of nematodes.

[0051] Optionally, the second pipe 4 is 12 cm long, with 2 cm of one end being conical, and the outlet 5 is located 6 cm away from the conical end.

[0052] It is understandable that the outlet 5 is designed so that when water is discharged, the water flow speed is relatively small, the impact and disturbance to the bottom are weak, and it is not easy to make the nematodes settled at the bottom float up or generate eddies.

[0053] In the embodiments provided by this utility model, the filter screen 3 is detachably connected to the first pipe 2 to facilitate the replacement of the filter screen 3.

[0054] In the embodiments provided by this utility model, a shell can be fitted over the outside of the nematode separator to keep the separator in a dark environment, and a small door can be opened on the shell to allow the sample to be placed into the funnel 1.

[0055] Furthermore, a lamp tube is provided at the top inside the shell, which can be turned on or off to provide a dark or light environment and to separate light-avoiding and light-attracting nematodes.

[0056] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0057] Although embodiments of this utility model have been disclosed above, it is not limited to the applications listed in the specification and embodiments. It can be applied to various fields suitable for this utility model. Other modifications can be readily implemented by those skilled in the art. Therefore, without departing from the general concept defined by the claims and their equivalents, this utility model is not limited to the specific details and examples shown and described herein.

Claims

1. A nematode separator, fixed on a support, characterized in that, Includes a funnel (1) and a filtration and separation pipe; The funnel (1) is detachable at the upper end of the filter separation pipe. The filter separation pipe is equipped with a filter screen (3) and the filter screen (3) is located below the funnel (1). The side wall of the filter separation pipe is equipped with a water outlet (5) and the water outlet (5) is located below the filter screen (3). The water outlet (5) is equipped with a valve (9). The lower end of the filter separation pipe is closed.

2. The nematode separator as described in claim 1, characterized in that, The outlet (5) is located at one end near the funnel (1).

3. The nematode separator as described in claim 1, characterized in that, The filtration and separation pipeline includes a first pipeline (2) and a second pipeline (4). The upper end of the first pipeline (2) is inserted into the funnel (1). The filter screen (3) is located inside the first pipeline (2). The lower end of the first pipeline (2) is detachably and sealed to the upper end of the second pipeline (4). The lower end of the second pipeline (4) is closed. The outlet (5) is located at the end of the second pipeline (4) near the funnel (1).

4. A nematode separator as described in claim 3, characterized in that, There are several second pipes (4), and each second pipe (4) is connected to the first pipe (2) through a diversion structure.

5. A nematode separator as described in claim 4, characterized in that, The diversion structure includes a multi-way diverter (6) and several water distribution pipes (7). One port of the multi-way diverter (6) is connected to the first pipe (2), and the other ports are connected to one end of several water distribution pipes (7) respectively. The other end of several water distribution pipes (7) is connected to several second pipes (4) respectively.

6. A nematode separator as described in claim 5, characterized in that, A third pipe (8) is provided between the second pipe (4) and the water distribution pipe (7).

7. A nematode separator as described in claim 3, characterized in that, The end of the second pipe away from the first pipe (2) is tapered.

8. A nematode separator as described in claim 1, characterized in that, The filter screen (3) is detachably connected to the filter separation pipe.

9. A nematode separator as described in claim 8, characterized in that, The filter (3) includes two layers of gauze and one layer of toilet paper, with the toilet paper layer located between the two layers of gauze and the gauze layer extending out of the upper port of the filter separation pipe.

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