A confirmation test device for detecting bacillus cereus

By designing a confirmatory experimental device for the detection of Bacillus cereus, and adopting a transparent detection box and an automated system, the problem of time-consuming and labor-intensive manual staining in the existing technology has been solved, and efficient and safe experimental operation has been achieved.

CN224313526UActive Publication Date: 2026-06-02LIAONING PROVINCIAL INSPECTION & TESTING CERTIFICATION CENT

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
LIAONING PROVINCIAL INSPECTION & TESTING CERTIFICATION CENT
Filing Date
2025-06-11
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing confirmatory assays for detecting Bacillus cereus require multiple manual staining processes, which are time-consuming, labor-intensive, and difficult to operate efficiently.

Method used

A confirmatory experimental device for detecting Bacillus cereus was designed, which uses a transparent detection box, magnetic base, syringe and air bag system to achieve automated staining and drying and reduce manual operation.

Benefits of technology

It enables a single person to quickly complete staining experiments, ensuring accurate staining volume, safe and efficient waste liquid collection, and improving experimental efficiency and effectiveness.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a kind of confirmation experimental devices for Bacillus cereus detection with wax sample, including transparent detection box, two magnetic seats are symmetrically installed in the upper end in transparent detection box, two magnetic seats upper end are fixed with glass slide by magnetic block, the upper end of transparent detection box is provided with several syringes, the lower end of syringe is connected with the upper end of hard long connecting pipe by pipe joint, the lower end of several long connecting pipes is located above glass slide, the uppermost of glass slide is also provided with horn head, the upper end of horn head is connected with air duct and air bag, the lower end of transparent detection box is set in the liquid collection box outside, compared with prior art, the beneficial effects of the utility model are: when carrying out confirmation experiment, each kind of reagent needed for dyeing is respectively loaded into different syringes, reagent is made to drop on glass slide by long connecting pipe by pushing syringe to carry out dyeing, air flow is generated by extruding air bag and acts on magnetic seat, realize air drying.
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Description

Technical Field

[0001] This utility model relates to the field of Bacillus cereus detection technology, specifically to a confirmatory experimental device for Bacillus cereus detection. Background Technology

[0002] In the detection of Bacillus cereus, confirmatory tests are necessary for the detected Bacillus cereus. Confirmatory tests are of great significance and necessity. They utilize various characteristic reactions, such as biochemical properties and serological reactions, to more accurately identify suspected Bacillus cereus from the initial screening. Furthermore, by utilizing the unique physiological and biochemical characteristics or genetic features of Bacillus cereus, it can be distinguished from other similar bacteria, avoiding misdiagnosis. Confirmatory tests significantly improve the reliability and accuracy of the detection results. Common confirmatory methods include Gram staining and spore observation. These methods share the characteristic of requiring repeated staining and drying of samples using multiple reagents. The repeated manual staining and drying of slides with staining agents is inconvenient and time-consuming, especially when handling large numbers of samples. Therefore, we propose... Utility Model Content

[0003] The purpose of this invention is to provide a confirmatory experimental device for detecting Bacillus cereus, so as to solve the problems mentioned in the background art.

[0004] A confirmatory experimental apparatus for detecting Bacillus cereus includes a transparent detection box. Two magnetic bases are symmetrically installed in the upper part of the transparent detection box. A glass slide is placed on the upper end of each magnetic base. Magnetic blocks are magnetically attracted to the upper ends of both sides of the glass slide, and the slide is fixed in place by the attraction between the magnetic blocks and the magnetic bases. Several syringes are provided at the upper part of the transparent detection box. The lower end of each syringe is connected to the upper end of a rigid long connecting tube made of metal or hard plastic via a connector. The lower end of the long connecting tube is inserted into the transparent detection box. Inside the transparent detection box, the lower ends of several long connecting tubes are positioned above the glass slide, ensuring that the liquid flowing from the long connecting tubes accurately acts on the glass slide. A horn head is also positioned directly above the glass slide, with its upper end connected to a gas guide tube. The upper end of the gas guide tube extends out of the transparent detection box and connects to the air bladder. A universal adapter is installed on the leftmost long connecting tube, allowing it a certain degree of rotation. The lower end of the transparent detection box is fitted over the liquid collection box, ensuring that all waste liquid can flow into the liquid collection box.

[0005] Preferably, the syringe connected to the leftmost long connecting tube has a larger capacity than the other syringes. The leftmost syringe is used to store disinfectant, and the surface of several syringes is provided with scale lines to facilitate control of the staining amount according to experimental needs.

[0006] Preferably, support blocks are symmetrically installed on both sides inside the liquid collection box, and a mesh plate is placed on the upper end of the support blocks. The mesh plate can prevent the alcohol lamp from being used for heating.

[0007] Preferably, the front end of the transparent testing box is equipped with an observation window, and opening and closing the observation window facilitates the placement and removal of glass slides.

[0008] Compared with the prior art, the beneficial effects of this utility model are:

[0009] During the confirmation experiment, the various reagents, sterile water, disinfectant and other materials required for staining are first loaded into different syringes. After the bacterial strain to be confirmed is transferred onto the glass slide, the glass slide is placed on the upper end of two magnetic bases and fixed by magnetic blocks. Then, by pushing the syringe, the staining agent in the syringe drips onto the glass slide through the long connecting tube for staining. By squeezing the air bladder, an airflow is generated, which acts on the magnetic base through the air guide tube and the horn head to achieve air drying.

[0010] The entire confirmation experiment can be easily and quickly completed by a single person, and the amount of staining can be accurately controlled, making it easy to observe the staining effect. The entire process does not involve contact with reagents, and the waste liquid generated in the experiment is directly collected in the collection box, ensuring the safety and efficiency of the experiment and effectively improving the efficiency and effect of the experiment. Attached Figure Description

[0011] Figure 1 This is the front view of the present invention;

[0012] Figure 2 This is a front structural cross-sectional view of the present invention.

[0013] In the diagram: 1. Transparent detection box, 2. Magnetic base, 3. Glass slide, 4. Magnetic block, 5. Syringe, 6. Tube connector, 7. Long connecting tube, 8. Horn head, 9. Air tube, 10. Airbag, 11. Universal adapter, 12. Liquid collection box, 13. Support block, 14. Mesh plate, 15. Scale line, 16. Observation window. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] See Figure 1-2A confirmatory experimental apparatus for detecting Bacillus cereus includes a transparent detection box 1. Two magnetic seats 2 are symmetrically installed in the upper part of the transparent detection box 1. A glass slide 3 is placed on the upper part of each magnetic seat 2. Magnetic blocks 4 are magnetically attracted to the upper sides of the glass slide 3, fixing the glass slide 3 in place by the attraction between the magnetic blocks 4 and the magnetic seats 2. Several syringes 5 are arranged in the upper part of the transparent detection box 1. The lower end of each syringe 5 is connected to the upper end of a rigid long connecting tube 7 via a connector 6. The long connecting tube 7 is made of metal or hard plastic. The lower end of the long connecting tube 7 is inserted into the transparent detection box 1, and the lower ends of several long connecting tubes 7 are all located above the glass slide 3, so that the liquid flowing from the long connecting tube 7 can accurately act on the glass slide. The syringe 5 connected to the leftmost long connecting tube 7 has a larger capacity than the other syringes 5. The syringe 5 is used to store disinfectant, and the surface of each syringe 5 is marked with graduation lines 15 to facilitate control of the staining amount according to experimental needs. A horn head 8 is also provided directly above the slide 3. The upper end of the horn head 8 is connected to the gas guide tube 9. The upper end of the gas guide tube 9 passes through the transparent detection box 1 and connects to the air bag 10. A universal adapter 11 is provided on the long connecting tube 7 located on the far left, so that the long connecting tube 7 has a certain degree of rotation freedom. The lower end of the transparent detection box 1 is fitted outside the liquid collection box 12 to ensure that all waste liquid can flow into the liquid collection box 12. Support blocks 13 are symmetrically installed on both sides inside the liquid collection box 12. A mesh plate 14 is placed on the upper end of the support block 13. The mesh plate 14 can prevent the alcohol lamp from being used for heating. An observation window 16 is installed at the front end of the transparent detection box 1. Opening and closing the observation window 16 facilitates the handling of the slide 3.

[0016] Working principle: During the confirmation experiment, various reagents, sterile water, disinfectant, etc. required for staining are first loaded into different syringes 5. After the bacteria to be confirmed are transferred onto the glass slide 3, the glass slide is placed on the upper end of the two magnetic seats 2 and fixed by the magnetic blocks 4. Then, by pushing the syringes 5, the staining agents in the different syringes 5 are dripped onto the glass slide 3 through the long connecting tube 7 according to the experimental steps. The air is generated by squeezing the air bag 10, and the air acts on the magnetic seat 2 through the air guide tube 9 and the horn head 8 to achieve air drying. Finally, the waste liquid generated by the experiment flows directly into the collection box 12 to ensure the safety and efficiency of the experiment.

[0017] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0018] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A confirmatory experimental apparatus for detecting Bacillus cereus, comprising a transparent detection chamber (1), characterized in that: Two magnetic bases (2) are symmetrically installed at the upper end of the transparent testing box (1). A glass slide (3) is placed on the upper end of the magnetic base (2). Magnetic blocks (4) are magnetically attracted to the upper ends of both sides of the glass slide (3). Several syringes (5) are provided at the upper end of the transparent testing box (1). The lower end of each syringe (5) is connected to the upper end of a rigid long connecting tube (7) via a tube connector (6). The lower end of the long connecting tube (7) is inserted into the transparent testing box (1). The lower ends of several long connecting tubes (7) are located above the glass slide (3). A horn head (8) is also provided directly above the glass slide (3). The upper end of the horn head (8) is connected to the air guide tube (9). The upper end of the air guide tube (9) passes through the transparent detection box (1) and connects to the air bag (10). A universal adapter (11) is provided on the leftmost long connecting tube (7). The lower end of the transparent detection box (1) is fitted outside the liquid collection box (12).

2. The confirmatory experimental apparatus for detecting Bacillus cereus according to claim 1, characterized in that: The syringe (5) connected to the leftmost long connecting tube (7) has a larger capacity than the other syringes (5), and the surfaces of several syringes (5) are provided with scale lines (15).

3. The confirmatory experimental apparatus for detecting Bacillus cereus according to claim 1, characterized in that: Support blocks (13) are symmetrically installed on both sides inside the liquid collection box (12), and a mesh plate (14) is placed on the upper end of the support block (13).

4. The confirmatory experimental apparatus for detecting Bacillus cereus according to claim 1, characterized in that: The front end of the transparent testing box (1) is equipped with an observation window (16).