A fully automatic flow pretreatment instrument

The design of a fully automated flow cytometry pretreatment instrument enables the automated processing of lymphocyte subsets and cytokines, solving the problem of simultaneous processing that cannot be achieved in existing technologies, improving operational efficiency and accuracy, and freeing up manpower.

CN224327970UActive Publication Date: 2026-06-05THE FIRST AFFILIATED HOSPITAL OF TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
Filing Date
2025-07-25
Publication Date
2026-06-05

AI Technical Summary

Technical Problem

Existing flow cytometry pretreatment instruments cannot process lymphocyte subsets and cytokines simultaneously, and cannot completely eliminate the need for human intervention, resulting in low operational efficiency and susceptibility to human error.

Method used

A fully automated flow cytometry pretreatment instrument was designed, comprising a sample loading component, a shaking incubation module, a centrifugation washing module, and a gripping component. Automated sample processing, including automatic sample loading, shaking incubation, and centrifugation washing, is achieved through a three-dimensional robotic arm and servo motors.

Benefits of technology

It enables simultaneous processing of lymphocyte subsets and cytokines, shortens pretreatment time, improves operational efficiency and sample loading accuracy, frees up manpower, and enhances the overall efficiency of flow cytometry detection.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model belongs to medical equipment technical field, specifically is a kind of full-automatic flow type pre-treatment instrument, including organism;The inside of the organism is equipped with sample adding assembly;The inside of the organism is equipped with oscillation incubation module;The inside of the organism is equipped with centrifugal washing module;The organism is equipped with locking assembly through sample adding assembly;The organism is equipped with clamping assembly through sample adding assembly;The organism is equipped with rotating assembly through sample adding assembly;Through the above structure, sample is moved to sample adding area according to procedure by grabbing component, and the setting of processing in oscillation incubation module and centrifugal washing module, constitute full-automatic flow type pre-treatment structure, satisfy lymphocyte subgroup and cytokine simultaneous processing, to shorten the pre-treatment time, improve the on-machine speed, shorten the entire flow cytometry detection procedure, improve the pre-treatment efficiency, and also greatly improve the accuracy of sample adding, provide strong support for subsequent experiment.
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Description

Technical Field

[0001] This utility model belongs to the field of medical equipment technology, specifically a fully automated flow pretreatment instrument. Background Technology

[0002] Pre-processing for flow cytometry involves steps such as antibody incubation and centrifugation washing, which currently rely heavily on manual operation. For laboratories with large sample volumes, this not only consumes manpower and has low operational efficiency, but also results in inconsistent sample processing quality, which is susceptible to human error.

[0003] The production of flow cytometry pretreatment instruments not only saves manpower but also ensures the consistency of sample loading, greatly improving the efficiency of flow cytometry experiments. However, flow cytometry pretreatment instruments currently on the market usually cannot process lymphocyte subsets and cytokines simultaneously, or they do not integrate centrifugation and washing modules, thus not completely freeing up manpower.

[0004] Therefore, this utility model provides a fully automated flow pretreatment instrument. Utility Model Content

[0005] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0006] Given that the existing technologies mentioned above have problems such as the fact that flow cytometry pretreatment instruments currently on the market usually cannot process lymphocyte subsets and cytokines at the same time, or that they cannot completely free up manpower by integrating centrifugation and washing modules.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A fully automated flow cytometry pretreatment instrument includes a body; a sample dispensing assembly is provided inside the body; a shaking incubation module is provided inside the body; a centrifugation washing module is provided inside the body; a locking assembly is provided through the sample dispensing assembly; a clamping assembly is provided through the sample dispensing assembly; a rotating assembly is provided through the sample dispensing assembly; and a gripping assembly is provided through the clamping assembly.

[0009] Preferably, the sample loading assembly includes a three-dimensional robotic arm, a base, a circular turntable, and a tube rack; the three-dimensional robotic arm is mounted on the top of the machine body; the base is rotatably connected to the top of the machine body via a servo motor; the circular turntable is detachably mounted on the top of the base; the tube rack is fixedly connected to the bottom of the circular turntable; multiple sets of tube racks are provided at the bottom of the circular turntable and are evenly distributed at the bottom of the circular turntable; the output end of the three-dimensional robotic arm is provided with a pair of sample loading heads.

[0010] Preferably, the locking assembly includes a locking block, a guide rod, and a first spring; the locking block is slidably connected to the top of the base near the circular turntable; multiple sets of locking blocks are provided on the top of the base and are evenly distributed on the top of the base; one end of the guide rod is slidably connected to the inner side wall of the locking block away from the circular turntable; the other end of the guide rod is fixedly connected to the inside of the base; and the first spring is sleeved on the outer side wall of the guide rod.

[0011] Preferably, the gripping component includes positioning holes, clamping posts, and slots; the positioning holes are located in the center of the circular turntable; multiple sets of positioning holes are provided in the center of the circular turntable and are evenly distributed in the center of the circular turntable; the clamping posts are installed in the center of the three-dimensional robotic arm by the gripping component, and multiple sets are provided; the slots are located on the sidewalls of the clamping posts that are close to each other.

[0012] Preferably, the rotating assembly includes a fixed frame, a first servo motor, and a rotating frame; the fixed frame is installed at the bottom of the three-dimensional robotic arm; the first servo motor is fixedly connected inside the fixed frame; the rotating frame is rotatably connected to the bottom of the fixed frame; and the top of the rotating frame is fixedly connected to the transmission end of the first servo motor.

[0013] Preferably, the clamping assembly includes a second servo motor, a winch, a pull rope, a slider, and a second spring; the second servo motor is fixedly connected to the top of the inner wall of the rotating frame; the winch is rotatably connected to the bottom of the inner wall of the rotating frame; the winch is fixedly connected to the drive end of the second servo motor; the slider is slidably connected to the bottom of the inner wall of the rotating frame; multiple sets of pull ropes are arranged symmetrically at the bottom of the inner wall of the rotating frame; the top of the clamping column is fixedly connected to the bottom of the pull rope; one end of the second spring is fixedly connected to the side wall of the pull rope away from the winch; the other end of the second spring is fixedly connected to the bottom of the inner wall of the rotating frame.

[0014] Preferably, the outer walls of the multiple sets of pipe racks are fitted with fixing hoops; the fixing hoops are made of metal.

[0015] Compared with the prior art, the beneficial effects of this utility model are:

[0016] 1. The fully automated flow cytometry pretreatment instrument described in this utility model uses a gripping component to move samples to the sample loading area according to a program. The oscillation incubation module and the centrifugation washing module are used for processing, forming a fully automated flow cytometry pretreatment structure. This allows for the simultaneous processing of lymphocyte subsets and cytokines, thereby shortening the pretreatment time, increasing the speed of sample loading, shortening the entire flow cytometry detection process, improving pretreatment efficiency, and significantly enhancing the accuracy of sample loading, providing strong support for subsequent experiments.

[0017] 2. The fully automatic flow cytometry pretreatment instrument described in this utility model uses a servo motor to drive the base, circular turntable and tube rack to rotate. The sample dispensing head is set to add samples to the flow cytometry tubes in sequence to form a sample dispensing structure, which realizes the function of adding reagents and samples to the flow cytometry tubes, solves the cumbersomeness of manual sample adding steps, and improves the convenience of sample dispensing. Attached Figure Description

[0018] Figure 1 This is a perspective view of the present invention;

[0019] Figure 2 This is a schematic diagram of the structure of the tube frame and the circular turntable of this utility model;

[0020] Figure 3 This is a schematic diagram of the structure of the card block and the circular turntable in this utility model;

[0021] Figure 4 This is a schematic diagram of the structure of the clamping column and the rotating frame in this utility model;

[0022] Figure 5 This is a schematic diagram of the guide rod and the first spring in this utility model.

[0023] In the diagram: 1. Body; 11. 3D robotic arm; 12. Base; 13. Circular turntable; 14. Pipe rack; 2. Clamping block; 21. Guide rod; 22. First spring; 3. Positioning hole; 31. Clamping column; 32. Slot; 4. Fixing frame; 41. First servo motor; 42. Rotating frame; 5. Second servo motor; 51. Winch; 52. Pull rope; 53. Slider; 54. Second spring; 6. Fixing clamp. Detailed Implementation

[0024] To make the above-mentioned objectives, features and advantages of this utility model more readily understood, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0025] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0026] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single embodiment or an embodiment selectively excluded from other embodiments.

[0027] Example 1:

[0028] Please see Figures 1-5 This is the first embodiment of the present invention.

[0029] This embodiment provides a fully automated flow cytometry pretreatment instrument, including a body 1; a sample dispensing assembly inside the body 1; a shaking incubation module inside the body 1; a centrifugation washing module inside the body 1; a locking assembly connected to the sample dispensing assembly; a clamping assembly connected to the sample dispensing assembly; a rotating assembly connected to the sample dispensing assembly; and a gripping assembly connected to the clamping assembly. During operation, the shaking incubation module has a magnetically coupled oscillation mechanism and a completely black treatment chamber. The centrifugation washing module is a low-speed centrifuge. In use, the sample is placed in a test tube rack, the program is started, the sample dispensing assembly inserts the appropriate pipette tip, and reagents and samples are added to the corresponding flow tube. After dispensing, the clamping assembly drives the gripping assembly to apply the sample to the sample within the sample dispensing assembly. The sample is grasped and moved to the light-protected shaking incubation module for light-protected shaking. After shaking, the sample is moved to the sample loading area, diluent is added, and after dilution, the sample is grasped and centrifuged in the centrifugation area. The liquid in the flow cytometer tube is then drained through the washing needle in the centrifugation washing module, and washing buffer is added, thus completing the pretreatment. Through the above structure, the grasping component moves the sample to the sample loading area according to the program, and the shaking incubation module and centrifugation washing module are set up to form a fully automated flow cytometry pretreatment structure. This structure can simultaneously process lymphocyte subsets and cytokines, thereby shortening the pretreatment time, increasing the speed of flow cytometry, shortening the entire flow cytometry detection process, improving pretreatment efficiency, and significantly improving the accuracy of sample loading, providing strong support for subsequent experiments.

[0030] Example 2:

[0031] Please see Figures 1-5 This is the second embodiment of the present utility model.

[0032] Specifically, such as Figure 1 and Figure 2As shown, the sample loading assembly includes a three-dimensional robotic arm 11, a base 12, a circular turntable 13, and a tube rack 14. The three-dimensional robotic arm 11 is mounted on the top of the machine body 1. The base 12 is rotatably connected to the top of the machine body 1 via a servo motor. The circular turntable 13 is detachably mounted on the top of the base 12. The tube rack 14 is fixedly connected to the bottom of the circular turntable 13. Multiple sets of tube racks 14 are set at the bottom of the circular turntable 13 and are evenly distributed at the bottom of the circular turntable 13. The output end of the three-dimensional robotic arm 11 is equipped with a pair of sample loading heads. During operation, the flow cytometer tube is placed into the tube in the middle of the circular turntable 13. Inside the rack 14, during sample addition, the three-dimensional robotic arm 11 moves, driving the sample addition head on the three-dimensional robotic arm 11 to the appropriate position. The servo motor drives the base 12 and the circular turntable 13 to rotate, adding samples to the flow cytometer tubes in the current workstation respectively. Through the above structure, the servo motor drives the base 12, the circular turntable 13 and the tube rack 14 to rotate, and the sample addition head is set to add samples to the flow cytometer tubes in sequence, forming a sample addition structure. This realizes the function of adding reagents and samples to the flow cytometer tubes, solving the cumbersomeness of manually adding samples and improving the convenience of sample addition.

[0033] like Figure 3 As shown, the locking assembly includes a locking block 2, a guide rod 21, and a first spring 22. The locking block 2 is slidably connected to the top of the base 12 near the circular turntable 13. Multiple sets of locking blocks 2 are arranged on the top of the base 12 and are evenly distributed. One end of the guide rod 21 is slidably connected to the inner wall of the locking block 2 away from the circular turntable 13. The other end of the guide rod 21 is fixedly connected to the inside of the base 12. The first spring 22 is sleeved on the outer wall of the guide rod 21. During operation, the sliding locking blocks 2 move closer to each other, and the locking blocks 2 slide on the outer wall of the guide rod 21, compressing the first spring 22. The circular turntable 13 is inserted into the top of the base 12, releasing the locking block 2. The first spring 22 drives the locking block 2 to move closer to the circular turntable 13, and the locking block 2 is inserted into the circular turntable 13, connecting the base 12 and the circular turntable 13 together. Through the above structure, the locking block 2 connects the base 12 and the circular turntable 13 together, forming a locking structure, which realizes the function of fixing the base 12 and the circular turntable 13 together, solving the problem of inconvenient cleaning of the circular turntable 13, improving the ease of disassembly and cleaning of the circular turntable 13.

[0034] like Figure 3 and Figure 4As shown, the gripping component includes a positioning hole 3, a clamping post 31, and a slot 32. The positioning hole 3 is located in the middle of the circular turntable 13. Multiple sets of positioning holes 3 are evenly distributed in the middle of the circular turntable 13. Multiple sets of clamping posts 31 are installed in the middle of the three-dimensional robotic arm 11 via the gripping component. The slot 32 is located on the sidewalls where the clamping posts 31 are close to each other. During operation, the three-dimensional robotic arm 11 moves the gripping component and the clamping posts 31 closer to the circular turntable 13, and moves the clamping posts 31 into the positioning hole 3. The gripping component moves the clamping posts 31 closer to each other, and the slot 32 is engaged inside the positioning hole 3, gripping the circular turntable 13. Through the above structure, the clamping posts 31 are inserted into the positioning hole 3 in the middle of the circular turntable 13, forming a gripping structure for the circular turntable 13. This achieves the function of gripping and fixing the circular turntable 13, improving the convenience of gripping and moving the circular turntable 13, and reducing the possibility of the circular turntable 13 falling off during gripping.

[0035] like Figure 4 As shown, the rotating assembly includes a fixed frame 4, a first servo motor 41, and a rotating frame 42. The fixed frame 4 is installed at the bottom of the three-dimensional robotic arm 11. The first servo motor 41 is fixedly connected inside the fixed frame 4. The rotating frame 42 is rotatably connected to the bottom of the fixed frame 4. The top of the rotating frame 42 is fixedly connected to the transmission end of the first servo motor 41. During operation, the three-dimensional robotic arm 11 moves the fixed frame 4 to a suitable position, starts the first servo motor 41, and drives the rotating frame 42 to rotate, thereby driving the clamping column 31 to rotate to a suitable angle. Through the above structure, the setting of the first servo motor 41 driving the rotating frame 42 and the clamping column 31 to rotate forms a rotating structure of the gripping assembly, realizing the function of driving the clamping column 31 to rotate, improving the convenience of positioning the clamping column 31, and increasing the accuracy of inserting the clamping column 31 into the positioning hole 3.

[0036] like Figure 4As shown, the clamping assembly includes a second servo motor 5, a winch 51, a pull rope 52, a slider 53, and a second spring 54. The second servo motor 5 is fixedly connected to the top of the inner wall of the rotating frame 42. The winch 51 is rotatably connected to the bottom of the inner wall of the rotating frame 42. The winch 51 is fixedly connected to the transmission end of the second servo motor 5. The slider 53 is slidably connected to the bottom of the inner wall of the rotating frame 42. Multiple sets of pull ropes 52 are arranged symmetrically on the bottom of the inner wall of the rotating frame 42. The top of the clamping post 31 is fixedly connected to the bottom of the pull rope 52. One end of the second spring 54 is fixedly connected to the side wall of the pull rope 52 away from the winch 51. The other end of the second spring 54 is fixedly connected to the bottom of the inner wall of the rotating frame 42. During operation, after the clamping post 31 is inserted into the positioning hole 3, the second servo motor 5 is started, and the second servo motor 5 drives... The winch 51 rotates, winding up multiple sets of pull ropes 52. The pull ropes 52 pull the sliders 53 closer together, and the sliders 53 drive the clamping posts 31 closer together, so that the slots 32 are locked inside the positioning holes 3. The second spring 54 is stretched. After the gripping component moves the circular turntable 13 to the appropriate position, the winch 51 is reversed, the pull ropes 52 become loose, and the second spring 54 drives the sliders 53 and clamping posts 31 to reset. Through the above structure, the winch 51 rotates to wind up the pull ropes 52 and drives the clamping posts 31 closer together, forming a clamping structure of the clamping posts 31. This realizes the function of driving the slots 32 to be locked inside the positioning holes 3, improving the stability of the clamping posts 31 when clamping the circular turntable 13, reducing the stability of the circular turntable 13 when transferring, and reducing the possibility of the circular turntable 13 falling off the clamping posts 31.

[0037] like Figure 2 As shown, the outer walls of the multiple sets of pipe racks 14 are fitted with fixing hoops 6; the fixing hoops 6 are made of metal; during operation, the fixing hoops 6 are fitted on the outer walls of the multiple sets of pipe racks 14 to connect the multiple sets of pipe racks 14 together, increase the integrity between the multiple sets of pipe racks 14, and improve the stability of the flow pipe when it is placed in the pipe rack 14.

[0038] During operation, the shaking incubation module features a magnetically coupled shaking mechanism and a completely blackened chamber. The centrifugation washing module is a low-speed centrifuge. In use, the sample is placed in the test tube rack, the program is started, the sample loading component inserts the appropriate pipette tip, and reagents and samples are added to the corresponding flow tube. After loading, the clamping component moves the gripping component to grip the sample in the loading component and moves it to the light-protected shaking incubation module for light-protected shaking. After shaking, the sample is moved to the loading area, diluent is added, and after dilution, the sample is gripped to the centrifugation area for centrifugation. The flow is then washed using the washing needle in the centrifugation washing module. After the liquid in the flow cytometry tube is drained and washing solution is added, the preparatory work is completed. The flow cytometry tube is placed in the tube rack 14 in the middle of the circular turntable 13. During sample addition, the three-dimensional robotic arm 11 moves, driving the sample addition head on the three-dimensional robotic arm 11 to move to the appropriate position. The servo motor drives the base 12 and the circular turntable 13 to rotate, adding samples to the flow cytometry tubes in the current station respectively. The sliding blocks 2 move closer to each other, sliding on the outer wall of the guide rod 21. The first spring 22 is compressed, inserting the circular turntable 13 into the top of the base 12. The blocks 2 are released, and the first spring 22 drives the blocks 2 to move closer to the circular turntable 13, inserting the blocks 2 into the circular turntable. Within 13, the base 12 and the circular turntable 13 are connected together; the three-dimensional robotic arm 11 moves the clamping assembly and the clamping column 31 closer to the circular turntable 13, and moves the clamping column 31 into the positioning hole 3. The clamping assembly moves the clamping columns 31 closer to each other, and the slot 32 is engaged inside the positioning hole 3 to grasp the circular turntable 13; the three-dimensional robotic arm 11 moves the fixing frame 4 to a suitable position, and starts the first servo motor 41. The first servo motor 41 drives the rotating frame 42 to rotate, thereby driving the clamping column 31 to rotate to a suitable angle; after the clamping column 31 is inserted into the positioning hole 3, the second servo motor 5 is started. Machine 5 drives winch 51 to rotate, winch 51 winds up multiple sets of pull ropes 52, pull ropes 52 pull sliders 53 closer together, sliders 53 drive clamping posts 31 closer together, so that the slot 32 is locked inside the positioning hole 3, the second spring 54 is stretched, after the gripping component drives the circular turntable 13 to move to the appropriate position, the winch 51 is reversed, the pull ropes 52 become loose, the second spring 54 drives sliders 53 and clamping posts 31 to reset; fixing clamp 6 is sleeved on the outer wall of multiple sets of pipe racks 14, connecting multiple sets of pipe racks 14 together, increasing the integrity between multiple sets of pipe racks 14, and improving the stability of the flow pipe when placed in the pipe rack 14.

[0039] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape, and proportions of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of this utility model. The order or sequence of any process or method steps may be changed or rearranged according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structural equivalents but also equivalent structures. Without departing from the scope of this invention, other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments. Therefore, this invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0040] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the present invention as currently considered, or those features that are not relevant to implementing the present invention) may be omitted.

[0041] It should be understood that numerous specific implementation decisions can be made during the development of any actual implementation method, and in any engineering or design project. Such development efforts may be complex and time-consuming, but for those of ordinary skill in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0042] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A fully automated flow pretreatment instrument, comprising a body (1); characterized in that: The machine body (1) is provided with a sample feeding component; the machine body (1) is provided with a shaking incubation module; the machine body (1) is provided with a centrifugal washing module; the machine body (1) is provided with a locking component through the sample feeding component; the machine body (1) is provided with a clamping component through the sample feeding component; the machine body (1) is provided with a rotating component through the sample feeding component; the machine body (1) is provided with a gripping component through the clamping component.

2. The fully automated flow cytometry pretreatment instrument according to claim 1, characterized in that: The sample loading assembly includes a three-dimensional robotic arm (11), a base (12), a circular turntable (13), and a tube rack (14); the three-dimensional robotic arm (11) is mounted on the top of the machine body (1); the base (12) is rotatably connected to the top of the machine body (1) via a servo motor; the circular turntable (13) is detachably mounted on the top of the base (12); the tube rack (14) is fixedly connected to the bottom of the circular turntable (13); multiple sets of the tube rack (14) are set at the bottom of the circular turntable (13) and are evenly distributed at the bottom of the circular turntable (13); the output end of the three-dimensional robotic arm (11) is provided with a pair of sample loading heads.

3. The fully automated flow cytometry pretreatment instrument according to claim 2, characterized in that: The locking assembly includes a locking block (2), a guide rod (21), and a first spring (22); the locking block (2) is slidably connected to the top of the base (12) near the circular turntable (13); multiple sets of locking blocks (2) are provided on the top of the base (12) and are evenly distributed on the top of the base (12); one end of the guide rod (21) is slidably connected to the inner side wall of the locking block (2) away from the circular turntable (13); the other end of the guide rod (21) is fixedly connected to the inside of the base (12); the first spring (22) is sleeved on the outer side wall of the guide rod (21).

4. The fully automated flow cytometry pretreatment instrument according to claim 3, characterized in that: The gripping assembly includes a positioning hole (3), a clamping post (31), and a slot (32); the positioning hole (3) is located in the middle of the circular turntable (13); multiple sets of the positioning holes (3) are provided in the middle of the circular turntable (13), and are evenly distributed in the middle of the circular turntable (13); the clamping post (31) is installed in the middle of the three-dimensional robotic arm (11) by the gripping assembly, and multiple sets are provided; the slot (32) is located on the side wall of the clamping post (31) that is close to each other.

5. The fully automated flow cytometry pretreatment instrument according to claim 4, characterized in that: The rotating assembly includes a fixed frame (4), a first servo motor (41), and a rotating frame (42); the fixed frame (4) is installed at the bottom of the three-dimensional robotic arm (11); the first servo motor (41) is fixedly connected inside the fixed frame (4); the rotating frame (42) is rotatably connected to the bottom of the fixed frame (4); the top of the rotating frame (42) is fixedly connected to the transmission end of the first servo motor (41).

6. The fully automated flow cytometry pretreatment instrument according to claim 5, characterized in that: The clamping assembly includes a second servo motor (5), a winch (51), a pull rope (52), a slider (53), and a second spring (54); the second servo motor (5) is fixedly connected to the top of the inner wall of the rotating frame (42); the winch (51) is rotatably connected to the bottom of the inner wall of the rotating frame (42); the winch (51) is fixedly connected to the transmission end of the second servo motor (5); and the slider (53) is slidably connected to the bottom of the inner wall of the rotating frame (42).

7. The fully automated flow cytometry pretreatment instrument according to claim 6, characterized in that: Multiple sets of the pull ropes (52) are arranged symmetrically on the bottom of the inner side wall of the rotating frame (42); the top of the clamping column (31) is fixedly connected to the bottom of the pull ropes (52); one end of the second spring (54) is fixedly connected to the side wall of the pull ropes (52) away from the winch (51); the other end of the second spring (54) is fixedly connected to the bottom of the inner side wall of the rotating frame (42).

8. The fully automated flow cytometry pretreatment instrument according to claim 2, characterized in that: The outer walls of the multiple sets of pipe racks (14) are fitted with fixing hoops (6); the fixing hoops (6) are made of metal.