A magnetic bead washing device

Abstract

This utility model relates to the field of in vitro diagnostic equipment technology, specifically to a magnetic bead cleaning device, including a mounting frame, a cleaning and conveying mechanism mounted on the mounting frame, a reaction cup mounted on the cleaning and conveying mechanism, a cleaning frame mounted on the mounting frame, a rinsing mechanism and a liquid aspiration mechanism sequentially mounted on the cleaning frame along the reaction cup conveying trajectory, and the liquid aspiration mechanism including a stand mounted on the cleaning frame, a lifting motor mounted on the rear end of the stand, a drive gear and a lifting gear mounted on the front end of the stand, a vertical slide rail mounted on the front end of the stand, a lifting frame slidably mounted on the vertical slide rail, a long slot mounted on the lifting frame, a limiting bolt mounted in the long slot and fixed to the lifting gear, and a liquid aspiration needle mounted on the lifting frame. This design has a simple structure, good cleaning effect, and convenient operation and control, enabling rinsing of the reaction cup and extraction of waste liquid.

Description

Technical Field

[0001] This utility model relates to the field of in vitro diagnostic equipment technology, specifically to a magnetic bead cleaning device. Background Technology

[0002] With the continuous expansion of the market, automated fluorescence immunoassay analyzers, high-sensitivity detection devices based on fluorescence labeling technology, are widely used in clinical diagnostics, biomedical research, drug development, and environmental monitoring. Their core technology utilizes the specific binding of fluorescent substances to antibodies or antigens, combined with an optical detection system, to achieve quantitative analysis of biomolecules. It can handle steps in the experimental testing process, including loading reaction cups, loading samples, loading reagent solutions, mixing, washing, enzyme-linked reactions, optical analysis, and computational analysis. The cleaning of magnetic beads within the automated fluorescence immunoassay analyzer is essential during the detection and analysis process. Existing magnetic bead cleaning mechanisms suffer from problems such as complex structure, poor cleaning effect, and low cleaning efficiency. Utility Model Content

[0003] To address the aforementioned problems, this invention provides a magnetic bead cleaning device with a simplified structure, significantly improving cleaning effect and efficiency.

[0004] The technical solution adopted by this utility model is to provide a magnetic bead cleaning device, including a mounting frame, a cleaning conveying mechanism disposed on the mounting frame, a reaction cup disposed on the cleaning conveying mechanism, a cleaning frame disposed on the mounting frame, a rinsing mechanism and a liquid suction mechanism sequentially disposed on the cleaning frame along the reaction cup conveying trajectory, wherein the liquid suction mechanism includes a vertical frame disposed on the cleaning frame, a lifting motor disposed on the rear end face of the vertical frame, a drive gear and a lifting gear disposed on the front end face of the vertical frame, a vertical slide rail disposed on the front end face of the vertical frame, a lifting frame slidably disposed on the vertical slide rail, an elongated slot disposed on the lifting frame, and a component disposed in the elongated slot and connected to... The lifting gear is fixed by a limiting bolt, and a suction needle is set on the lifting frame. The suction needle is vertically downward and corresponds to the upper opening of the reaction cup. The lifting motor passes through the upright and is connected to the drive gear. The lifting gear is rotatably mounted on the upright and meshes with the drive gear. The long slot is horizontally arranged. The limiting bolt is inserted into the long slot and fixed at an eccentric position on the outer end face of the lifting gear. The lifting frame is slidably mounted on the vertical slide rail. The lifting motor drives the drive gear to rotate, and the drive gear drives the lifting gear to rotate. The lifting gear drives the lifting frame to move up and down on the lifting slide rail through the limiting bolt.

[0005] The liquid suction mechanism also includes a limiting seat with a through hole mounted on the lifting frame, a fixed seat mounted on the liquid suction needle and located above or below the limiting seat, and a connecting spring mounted between the limiting seat and the fixed seat. The liquid suction needle is slidably inserted into the through hole of the limiting seat.

[0006] The rinsing mechanism includes a solenoid valve mounted on a cleaning rack, an inlet pipe mounted on the cleaning rack and connected to the input end of the solenoid valve, and an outlet pipe mounted on the cleaning rack and connected to the output end of the solenoid valve. The outlet end of the outlet pipe is vertically downward and corresponds to the opening at the top of the reaction cup.

[0007] The cleaning and conveying mechanism includes a cleaning disc rotatably mounted on a mounting frame, a support frame mounted at the lower end of the cleaning disc, a conveying motor mounted on the mounting frame, a conveying gear mounted at the output end of the conveying motor, teeth on the outer periphery of the cleaning disc, and a limiting wheel rotatably mounted on the mounting frame and corresponding to the inner periphery of the cleaning disc. The cleaning disc has a circular structure, the outer periphery of the limiting wheel is provided with an annular groove, the inner periphery of the cleaning disc is limited within the annular groove of the limiting wheel, and the outer periphery meshes with the conveying gear through teeth. The reaction cup is placed between the support frames, and the cleaning disc is provided with a clearance opening corresponding to the reaction cup. The lower end of the aspiration needle corresponds to the clearance opening.

[0008] The stacking rack includes a fixing plate fixed to the lower end face of the washing tray, a stacking seat fixed to the lower end of the fixing plate, and stacking slots provided on both sides of the stacking seat, with the stacking slots of adjacent stacking seats corresponding to each other.

[0009] It also includes a ring magnet mounted on the mounting bracket and located on one side of the bottom of the reaction vessel.

[0010] It also includes a heating film mounted on the mounting bracket and corresponding to the lower end of the reaction vessel.

[0011] The beneficial effects of this utility model are that it provides a magnetic bead cleaning device. In this device, when the reaction cup is conveyed to the lower end of the rinsing mechanism on the cleaning conveying mechanism, the rinsing mechanism sprays cleaning fluid into the reaction cup to rinse it. After the reaction cup continues to move to the lower end of the suction needle, the lifting motor drives the suction needle to descend and insert it into the reaction cup to absorb the waste liquid inside. This design has a simple structure, good cleaning effect, and convenient operation and control. It can realize the rinsing of the reaction cup and the extraction of waste liquid. This design can be set up in multiple groups, and multiple different cleaning solutions can be used to rinse the reaction cup multiple times, significantly improving the cleaning effect. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of this utility model;

[0013] Figure 2 This is a schematic diagram of the liquid suction mechanism and the rinsing mechanism;

[0014] Figure 3 This is a schematic diagram of the liquid suction mechanism and the rinsing mechanism from another direction;

[0015] Figure 4 This is a schematic diagram of the cleaning disc;

[0016] Figure 5 This is a schematic diagram of the structure of the support frame and reaction vessel;

[0017] Figure 6 yes Figure 4 A magnified view of A in the middle.

[0018] In the attached diagram, 1 is the mounting bracket, 2 is the reaction cup, 3 is the cleaning rack, 4 is the upright frame, 5 is the lifting motor, 6 is the drive gear, 7 is the lifting gear, 8 is the vertical slide rail, 9 is the lifting frame, 10 is the elongated slot, 11 is the limiting bolt, 12 is the suction needle, 13 is the limiting seat, 14 is the fixing seat, 15 is the connecting spring, 16 is the solenoid valve, 17 is the inlet pipe, 18 is the outlet pipe, 19 is the cleaning tray, 20 is the stacking frame, 21 is the conveying motor, 22 is the conveying gear, 23 is the limiting wheel, 24 is the fixing plate, 25 is the stacking seat, 26 is the stacking groove, 27 is the ring magnet, and 28 is the heating diaphragm. Detailed Implementation

[0019] like Figure 1-6 As shown, this utility model provides a magnetic bead cleaning device, including a mounting frame 1, a cleaning conveying mechanism mounted on the mounting frame 1, a reaction cup 2 mounted on the cleaning conveying mechanism, a cleaning frame 3 mounted on the mounting frame 1, a rinsing mechanism and a liquid suction mechanism sequentially mounted on the cleaning frame 3 along the conveying trajectory of the reaction cup 2. The liquid suction mechanism includes a stand 4 mounted on the cleaning frame 3, a lifting motor 5 mounted on the rear end face of the stand 4, a drive gear 6 and a lifting gear 7 mounted on the front end face of the stand 4, a vertical slide rail 8 mounted on the front end face of the stand 4, a lifting frame 9 slidably mounted on the vertical slide rail 8, an elongated slot 10 mounted on the lifting frame 9, and a component disposed within the elongated slot 10 and fixed to the lifting gear 7. The lifting frame 9 includes a limiting bolt 11 and a suction needle 12 mounted on the lifting frame 9. The suction needle 12 is vertically downward and corresponds to the upper opening of the reaction cup 2. The lifting motor 5 passes through the upright frame 4 and is connected to the drive gear 6. The lifting gear 7 is rotatably mounted on the upright frame 4 and meshes with the drive gear 6. The elongated slot 10 is horizontally arranged. The limiting bolt 11 is inserted into the elongated slot 10 and fixed at an eccentric position on the outer end face of the lifting gear 7. The lifting frame 9 is slidably mounted on the vertical slide rail 8. The lifting motor 5 drives the drive gear 6 to rotate, and the drive gear 6 drives the lifting gear 7 to rotate. The lifting gear 7 drives the lifting frame 9 to move up and down on the lifting slide rail through the limiting bolt 11.

[0020] The reaction cup 2 is a conical structure with an open top and a convex rim around the opening. The reaction cup 2 is conveyed by the cleaning and conveying mechanism, passing under the rinsing and suction mechanisms. In the suction mechanism, the output shaft of the lifting motor 5 passes through the upright frame 4, and water is suspended at the rear end of the upright frame 4. A drive gear 6 is mounted on this output shaft. The lifting gear 7 is rotatably mounted, meshing with the drive gear 6 to form a transmission connection. The elongated slot 10 is a horizontally elongated hole. The end of the limiting bolt 11 passes through the elongated slot 10 and is fixed at an eccentric position on the lifting gear 7. The limiting bolt 11 does not lock the lifting frame 9 to the lifting gear 7; it only limits the movement. The head size of the limiting bolt 11 is larger than the width of the elongated slot 10. The sliding connection between the vertical slide rail 8 and the lifting frame 9 is not limited, as long as the lifting frame 9 can slide and rise on the vertical slide rail 8 without disengaging. For example, the vertical slide rail 8 has a dovetail-shaped cross-section, and the lifting frame 9 has a matching dovetail groove. The upper end of the suction needle 12 is connected to the liquid circuit system of the equipment, and the liquid circuit system can use the suction needle 12 to remove the waste liquid in the reaction cup 2.

[0021] In use, when reaction cup 2 is conveyed to the lower end of the rinsing mechanism on the cleaning conveying mechanism, the rinsing mechanism sprays cleaning fluid into reaction cup 2 to rinse it. After rinsing, reaction cup 2 continues to move to the lower end of suction needle 12. The lifting motor 5 drives the drive gear 6 to rotate, which in turn drives the lifting gear 7 to rotate. The lifting gear 7, through the limit bolt 11, drives the lifting frame 9 to descend on the vertical slide rail 8. Suction needle 12 descends synchronously with the lifting frame 9, and its lower end extends into reaction cup 2 to suck up the waste liquid inside. After suction, suction needle 12 returns to its original position, and reaction cup 2 continues to be conveyed by the cleaning conveying mechanism. This design has a simple structure, good cleaning effect, and convenient operation and control. It can realize the rinsing of reaction cup 2 and the extraction of waste liquid. This design can be set up in multiple groups to perform multiple rinsings on reaction cup 2, greatly improving the cleaning effect.

[0022] like Figure 2-3 As shown, the liquid suction mechanism also includes a limiting seat 13 with a through hole on the lifting frame 9, a fixing seat 14 that is fitted and fixed on the liquid suction needle 12 and located above or below the limiting seat 13, and a connecting spring 15 disposed between the limiting seat 13 and the fixing seat 14. The liquid suction needle 12 is slidably inserted into the through hole of the limiting seat 13.

[0023] The aspiration needle 12 adopts a floating installation method. The aspiration needle 12 is limited by being inserted into the through hole of the limiting seat 13. The aspiration needle 12 can move up and down within the limiting seat 13. A fixing seat 14 is fixedly installed on the aspiration needle 12. The fixing seat 14 is connected to the limiting seat 13 by a connecting spring 15. The aspiration needle 12 has a floating function after being subjected to force, which can allow the aspiration needle 12 to extend into the reaction cup 2 until it touches the bottom of the reaction cup 2, so that the liquid is absorbed more thoroughly and without damaging the reaction cup 2.

[0024] like Figure 2-3 As shown, the rinsing mechanism includes a solenoid valve 16 mounted on the cleaning rack 3, an inlet pipe 17 mounted on the cleaning rack 3 and connected to the input end of the solenoid valve 16, and an outlet pipe 18 mounted on the cleaning rack 3 and connected to the output end of the solenoid valve 16. The outlet end of the outlet pipe 18 is vertically downward and corresponds to the opening at the upper end of the reaction cup 2.

[0025] The inlet pipe 17 is connected to the liquid circuit system of the equipment. The cleaning fluid is delivered to the inlet pipe 17 through the liquid circuit system. Under the control of the solenoid valve 16, it is sprayed downward into the reaction cup 2 through the outlet pipe 18 to achieve rinsing of the reaction cup 2. The structure is simple and effective, easy to control, and low in cost.

[0026] like Figure 4-5 As shown, the cleaning and conveying mechanism includes a cleaning disc 19 rotatably mounted on a mounting frame 1, a support frame 20 mounted at the lower end of the cleaning disc 19, a conveying motor 21 mounted on the mounting frame 1, a conveying gear 22 mounted at the output end of the conveying motor 21, teeth on the outer periphery of the cleaning disc 19, and a limiting wheel 23 rotatably mounted on the mounting frame 1 and corresponding to the inner periphery of the cleaning disc 19. The cleaning disc 19 has a circular structure, and the outer periphery of the limiting wheel 23 is provided with an annular groove. The inner periphery of the cleaning disc 19 is limited within the annular groove of the limiting wheel 23, and the outer periphery meshes with the conveying gear 22 by means of teeth. The reaction cup 2 is arranged between the support frames 20, and the cleaning disc 19 is provided with an avoidance opening corresponding to the reaction cup 2. The lower end of the suction needle 12 corresponds to the avoidance opening.

[0027] The annular cleaning disc 19 is limited by the rotating limiting wheel 23 and the conveying gear 22. The conveying motor 21 drives the conveying gear 22 to rotate, causing the cleaning disc 19 to rotate. The reaction cup 2 is placed between the support frame 20 at the lower end of the cleaning disc 19 and moves in an annular motion with the rotation of the cleaning disc 19. The cleaning and conveying mechanism is an existing mechanism. The cleaning disc 19 is designed with a clearance opening corresponding to the reaction cup 2, which does not hinder the suction of the aspiration needle 12 and the rinsing of the liquid outlet pipe 18.

[0028] like Figure 5As shown, the stacking rack 20 includes a fixing plate 24 fixed to the lower end face of the cleaning tray 19, a stacking seat 25 fixed to the lower end of the fixing plate 24, and stacking grooves 26 provided on both sides of the stacking seat 25, with the stacking grooves 26 of adjacent stacking seats 25 corresponding to each other. The outer rim of the upper opening of the reaction cup 2 rests in the stacking groove 26 on the side of the adjacent stacking seat 25.

[0029] like Figure 6 As shown, it also includes a ring magnet 27 disposed on the mounting bracket 1 and located on one side of the bottom of the reaction cup 2. The ring magnet 27 can attract the magnetic beads inside the reaction cup 2, adsorbing the magnetic beads on one side of the bottom of the reaction cup 2, preventing the magnetic beads from being sucked away by the liquid suction needle 12 or washed out of the reaction cup 2 during rinsing.

[0030] like Figure 6 As shown, it also includes a heating film 28 disposed on the mounting bracket 1 and corresponding to the lower end of the reaction vessel 2. The heating film 28 can heat the reaction vessel 2, keeping the cleaning solution inside the reaction vessel 2 at a suitable cleaning temperature, thereby further improving the cleaning effect.

Claims

1. A magnetic bead cleaning device, comprising a mounting frame (1), a cleaning conveying mechanism disposed on the mounting frame (1), a reaction cup (2) disposed on the cleaning conveying mechanism, a cleaning rack (3) disposed on the mounting frame (1), a rinsing mechanism and a liquid suction mechanism sequentially disposed on the cleaning rack (3) along the conveying trajectory of the reaction cup (2), characterized in that: The liquid suction mechanism includes a stand (4) mounted on the cleaning rack (3), a lifting motor (5) mounted on the rear end face of the stand (4), a drive gear (6) and a lifting gear (7) mounted on the front end face of the stand (4), a vertical slide rail (8) mounted on the front end face of the stand (4), a lifting frame (9) slidably mounted on the vertical slide rail (8), a long slot (10) mounted on the lifting frame (9), a limiting bolt (11) mounted in the long slot (10) and fixed to the lifting gear (7), and a liquid suction needle (12) mounted on the lifting frame (9). The aspiration needle (12) is vertically downward and corresponds to the upper opening of the reaction cup (2). The lifting motor (5) passes through the stand (4) and is connected to the drive gear (6). The lifting gear (7) is rotatably mounted on the stand (4) and meshes with the drive gear (6). The long slot (10) is horizontally arranged. The limiting bolt (11) is inserted into the long slot (10) and fixed at the eccentric position on the outer end face of the lifting gear (7). The lifting frame (9) is slidably mounted on the vertical slide rail (8). The lifting motor (5) drives the drive gear (6) to rotate. The drive gear (6) drives the lifting gear (7) to rotate. The lifting gear (7) drives the lifting frame (9) to move up and down on the lifting slide rail through the limiting bolt (11).

2. The magnetic bead cleaning device according to claim 1, characterized in that: The liquid suction mechanism also includes a limiting seat (13) with a through hole on the lifting frame (9), a fixing seat (14) fixed on the liquid suction needle (12) and located above or below the limiting seat (13), and a connecting spring (15) between the limiting seat (13) and the fixing seat (14). The liquid suction needle (12) is slidably inserted into the through hole of the limiting seat (13).

3. The magnetic bead cleaning device according to claim 1, characterized in that: The rinsing mechanism includes a solenoid valve (16) mounted on the cleaning rack (3), an inlet pipe (17) mounted on the cleaning rack (3) and connected to the input end of the solenoid valve (16), and an outlet pipe (18) mounted on the cleaning rack (3) and connected to the output end of the solenoid valve (16). The outlet end of the outlet pipe (18) is vertically downward and corresponds to the opening at the upper end of the reaction cup (2).

4. A magnetic bead cleaning device according to claim 1 or 3, characterized in that: The cleaning and conveying mechanism includes a cleaning disc (19) rotatably mounted on a mounting frame (1), a support frame (20) mounted at the lower end of the cleaning disc (19), a conveying motor (21) mounted on the mounting frame (1), a conveying gear (22) mounted at the output end of the conveying motor (21), teeth mounted on the outer periphery of the cleaning disc (19), and a limiting wheel (23) rotatably mounted on the mounting frame (1) and corresponding to the inner periphery of the cleaning disc (19). The cleaning tray (19) has a circular structure. The outer circumference of the limiting wheel (23) is provided with an annular groove. The inner circumference of the cleaning tray (19) is limited in the annular groove of the limiting wheel (23), and the outer circumference meshes with the conveying gear (22) by means of teeth. The reaction cup (2) is arranged between the stacking frame (20). The cleaning tray (19) is provided with a clearance port corresponding to the reaction cup (2), and the lower end of the liquid suction needle (12) corresponds to the clearance port.

5. The magnetic bead cleaning device according to claim 4, characterized in that: The stacking rack (20) includes a fixing plate (24) fixed to the lower end face of the cleaning tray (19), a stacking seat (25) fixed to the lower end of the fixing plate (24), and stacking slots (26) provided on both sides of the stacking seat (25), with the stacking slots (26) of adjacent stacking seats (25) corresponding to each other.

6. The magnetic bead cleaning device according to claim 1, characterized in that: It also includes a ring magnet (27) mounted on the mounting bracket (1) and located on one side of the bottom of the reaction cup (2).

7. The magnetic bead cleaning device according to claim 1, characterized in that: It also includes a heating film (28) disposed on the mounting bracket (1) and corresponding to the lower end of the reaction cup (2).

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