A nucleic acid reagent separator for isothermal amplification

Abstract

The utility model relates to the field of separator, specifically is a kind of constant temperature amplification nucleic acid reagent separator, including support component, support component includes support disc, the upper portion of support disc is equipped with the separation component for separating nucleic acid reagent, separation component includes the separator main body detachably installed in the upper portion of support disc, the outside of the separator main body is equipped with the temperature control assembly for controlling the temperature in the separator, the temperature control assembly includes the bearing fixedly installed in the side of the separator main body, the outside of bearing is fixedly installed with temperature control cover, the side of temperature control cover is fixedly installed with air inlet pipe, the inside and outside of air inlet pipe are jointly installed with air inlet valve, the utility model controls the temperature in the separator by adding nitrogen gas of different temperature to the inside of separator main body, ensure the temperature of nucleic acid reagent normal when separating.

Description

Technical Field

[0001] This utility model relates to the field of separator technology, specifically a isothermal amplification nucleic acid reagent separator. Background Technology

[0002] A separator is a machine that separates a mixture of substances into two or more different substances. Common separators include centrifugal separators and electrostatic separators. To maintain good separation efficiency, the liquid level and pressure of a separator need to be controlled. Traditional separators use constant pressure control technology for liquid level and pressure control. In variable pressure liquid level control of separators, a float-type liquid level controller drives oil and gas regulating valves, causing them to operate in conjunction to control the flow of crude oil and natural gas, thereby regulating the liquid level in the separator without controlling the separator pressure. The variable pressure liquid level control method can minimize the throttling of the oil and gas outlet valves, reduce the separator pressure, and improve the separation effect. Oil-gas separators and oil-gas-water three-phase separators are widely used in oilfield transfer stations and combined stations.

[0003] A search revealed a isothermal amplification nucleic acid reagent separator with publication number CN222182278U. This separator, using four separator bodies, achieves separation of isothermal amplification nucleic acid reagent test tubes, which is simple and convenient; after placing the test tubes, the separator bodies are simply inserted into the slots on the separation chamber. However, while the aforementioned isothermal amplification nucleic acid reagent separator achieves separation of isothermal amplification nucleic acid reagent test tubes with four separator bodies, it does not consider the temperature requirements during nucleic acid reagent separation and does not control the internal temperature of the separator. Therefore, we provide an isothermal amplification nucleic acid reagent separator. Utility Model Content

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0005] Given that the above or existing technologies do not take into account the temperature requirements during the nucleic acid reagent separation process, and do not control the temperature inside the separator.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A isothermal amplification nucleic acid reagent separator includes: a support assembly, the support assembly including a support disk, a separation assembly for separating nucleic acid reagents mounted above the support disk, the separation assembly including a separator body detachably mounted above the support disk, a temperature control assembly for controlling the internal temperature of the separator mounted on the outside of the separator body, the temperature control assembly including a bearing fixedly mounted on the side of the separator body;

[0008] A temperature control cover is fixedly installed on the outer side of the bearing, an air inlet pipe is fixedly installed on the side of the temperature control cover, an air inlet valve is installed inside and outside the air inlet pipe, a temperature sensor is installed on the top of the inner side of the temperature control cover, a nitrogen tank is opened inside the separator body, an air outlet valve is installed inside the separator body, and an air outlet is opened on the side of the separator body.

[0009] As a further embodiment of this utility model: a separation groove is provided on the side of the separator body, a separation box is detachably installed inside the separation groove, and a test tube groove is provided at the top of the separation box.

[0010] As a further improvement of this utility model: a push-pull groove is provided on the side of the separation box, a limit post is fixedly installed on the side of the separation box, and a limit groove is provided on the side of the separator body.

[0011] As a further embodiment of this utility model: a support block is fixedly installed on the side of the separator body, and a fixing ring is detachably installed on the outer side of the separator body above the support block.

[0012] As a further embodiment of this utility model: a support column is fixedly installed below the support plate, and a support foot is fixedly installed below the support column.

[0013] As a further improvement of this utility model: a slot is provided at the top of the support plate, and a square support column can be detachably installed inside the slot.

[0014] As a further embodiment of this utility model: a motor assembly is installed at the bottom of the support plate, the motor assembly includes a rotary motor fixedly installed at the bottom of the support plate, a transmission shaft is fixedly installed at the output end of the rotary motor, and a cross column is fixedly installed at the end of the transmission shaft.

[0015] As a further improvement of this utility model, a controller is fixedly installed on the top of the temperature control cover.

[0016] Compared with the prior art, the beneficial effects of this utility model are:

[0017] This invention controls the internal temperature of the separator by adding nitrogen gas at different temperatures to the separator body, ensuring that the nucleic acid reagent is at a normal temperature during separation.

[0018] This invention utilizes the centrifugal force during rotation to separate nucleic acid reagents. A fixing ring prevents the separation chamber from the separator body during rotation due to centrifugal force. A controller can control the status of the valves and motor. A support assembly can fix the entire device. A motor assembly can rotate the separation assembly to separate nucleic acid reagents. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of a nucleic acid reagent separator for isothermal amplification.

[0020] Figure 2 A detailed structural diagram of a nucleic acid reagent separator for isothermal amplification;

[0021] Figure 3 This is a schematic diagram of the temperature control component structure of an isothermal amplification nucleic acid reagent separator.

[0022] Figure 4 This is a schematic diagram of the separation component structure of an isothermal amplification nucleic acid reagent separator;

[0023] Figure 5 This is a schematic diagram of the support component and motor component in an isothermal amplification nucleic acid reagent separator.

[0024] In the diagram: 1. Support assembly; 11. Support plate; 12. Support column; 13. Support foot; 14. Slot; 15. Square support column; 2. Motor assembly; 21. Rotary motor; 22. Drive shaft; 23. Cross column; 3. Separation assembly; 31. Separator body; 32. Separation tank; 33. Separation box; 34. Test tube tank; 35. Push-pull groove; 36. Limiting column; 37. Limiting groove; 38. Support block; 39. Fixing ring; 4. Temperature control assembly; 41. Temperature control cover; 42. Temperature sensor; 43. Inlet pipe; 44. Inlet valve; 45. Outlet valve; 46. Outlet; 47. Nitrogen tank; 48. Bearing; 5. Controller. Detailed Implementation

[0025] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0026] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0027] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0028] Please see Figures 1 to 4 This is the first embodiment of the present invention. This embodiment provides a isothermal amplification nucleic acid reagent separator, including: a support component 1, the support component 1 including a support plate 11, a separation component 3 for separating nucleic acid reagents installed above the support plate 11, the separation component 3 including a separator body 31 detachably installed above the support plate 11, a temperature control component 4 for controlling the internal temperature of the separator installed on the outside of the separator body 31, and the temperature control component 4 including a bearing 48 fixedly installed on the side of the separator body 31;

[0029] A temperature control cover 41 is fixedly installed on the outer side of the bearing 48. An air inlet pipe 43 is fixedly installed on the side of the temperature control cover 41. An air inlet valve 44 is installed both inside and outside the air inlet pipe 43. A temperature sensor 42 is installed on the top of the inner side of the temperature control cover 41. A nitrogen tank 47 is opened inside the separator body 31. An air outlet valve 45 is installed inside the separator body 31. An air outlet 46 is opened on the side of the separator body 31.

[0030] Specifically, a separation groove 32 is provided on the side of the separator body 31, a separation box 33 is detachably installed inside the separation groove 32, a test tube groove 34 is provided at the top of the separation box 33, a push-pull groove 35 is provided on the side of the separation box 33, a limit post 36 is fixedly installed on the side of the separation box 33, a limit groove 37 is provided on the side of the separator body 31, a support block 38 is fixedly installed on the side of the separator body 31, and a fixing ring 39 is detachably installed on the outer side of the separator body 31 above the support block 38.

[0031] Furthermore, the nucleic acid reagents can be separated by the centrifugal force during rotation. The fixing ring 39 is secured to the side of the separation box 33 to prevent the separation box from the separator body 31 due to the centrifugal force during rotation.

[0032] In use, the temperature sensor 42 fixed on the temperature control cover 41 detects the internal temperature of the separator. Nitrogen gas at the required temperature is introduced into the nitrogen tank 47 inside the separator body 31 through the air inlet pipe 43. Excess gas is discharged through the air outlet 46. The gas content inside the separator body 31 is controlled by the air inlet valve 44 and the air outlet valve 45. The temperature control cover 41 is connected to the separator body 31 through the bearing 48. The test tube is placed inside the test tube tank 34. The separation box 33 is aligned with the separation tank 32 through the push-pull groove 35. The limiting post 36 on the side of the separation box 33 is aligned with the limiting groove 37 to fix the separation box 33 to the side of the separator body 31. The fixing ring 39 prevents the separation box 33 from dislodging from the separation tank 32 during rotation. The support block 38 prevents the fixing ring 39 from falling off.

[0033] In summary, the centrifugal force during rotation allows the nucleic acid reagents to be separated. The fixing ring 39 is secured to the side of the separation chamber 33 to prevent the separation chamber from the separator body 31 due to centrifugal force during rotation. The internal temperature of the separator is controlled by adding nitrogen gas at different temperatures into the separator body 31 to ensure that the temperature of the nucleic acid reagents is normal during separation.

[0034] Please see Figure 1 , Figure 2 and Figure 5 This is the second embodiment of the present invention, which provides an improved design for a isothermal amplification nucleic acid reagent separator.

[0035] Specifically, a support column 12 is fixedly installed below the support plate 11, and a support foot 13 is fixedly installed below the support column 12. A slot 14 is opened at the top of the support plate 11, and a square support column 15 is detachably installed inside the slot 14. A motor assembly 2 is installed at the bottom of the support plate 11. The motor assembly 2 includes a rotary motor 21 fixedly installed at the bottom of the support plate 11. A drive shaft 22 is fixedly installed at the output end of the rotary motor 21, and a cross column 23 is fixedly installed at the end of the drive shaft 22. A controller 5 is fixedly installed at the top of the temperature control cover 41.

[0036] Furthermore, the entire device can be fixed by the support component 1, and the separation component 3 can be rotated by the motor component 2 to separate nucleic acid reagents.

[0037] In use, the support plate 11 cooperates with the support column 12 and the support foot 13 to support and fix the separator. The slot 14 cooperates with the square support column 15 to fix the temperature control cover 41 above the support plate 11. The output end of the rotary motor 21 drives the drive shaft 22 to rotate, the drive shaft 22 drives the cross column 23 to rotate, and the cross column 23 drives the separator body 31 to rotate. The rotation of the separator body 31 drives the test tube inside the separation box 33 to rotate. During the rotation, due to the influence of centrifugal force, the nucleic acid reagent inside the test tube separates into layers, thereby achieving the separation effect. The controller 5 can display the data of the temperature sensor 42 and control the working status of the air inlet valve 44, the air outlet valve 45, and the rotary motor 21.

[0038] In summary, the controller 5 can control the state of the valve and motor, the support component 1 can fix the entire device, and the motor component 2 can rotate the separation component 3 to separate nucleic acid reagents.

[0039] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape and proportion of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of this utility model. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structural equivalents but also equivalent structures. Without departing from the scope of this invention, other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments. Therefore, this invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0040] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the present invention as currently considered, or those features that are not relevant to implementing the present invention) may be omitted.

[0041] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0042] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A isothermal amplification nucleic acid reagent separator, characterized in that, include: The support assembly includes a support plate on which a separation component for separating nucleic acid reagents is installed. The separation component includes a separator body that is detachably installed on the support plate. A nitrogen tank is opened inside the separator body. An exhaust valve is installed inside the separator body. An exhaust port is opened on the side of the separator body. A temperature control assembly for controlling the internal temperature of the separator is installed on the outside of the separator body. The temperature control assembly includes a bearing fixedly installed on the side of the separator body, a temperature control cover fixedly installed on the outside of the bearing, an air inlet pipe fixedly installed on the side of the temperature control cover, an air inlet valve installed inside and outside the air inlet pipe, and a temperature sensor installed on the top of the inner side of the temperature control cover.

2. The isothermal amplification nucleic acid reagent separator according to claim 1, characterized in that, The separator body has a separation groove on its side, and a separation box can be detachably installed inside the separation groove. A test tube groove is provided at the top of the separation box.

3. The isothermal amplification nucleic acid reagent separator according to claim 2, characterized in that, The side of the separator has a push-pull groove; Limiting posts are fixedly installed on the side of the separation box; Limiting grooves are provided on the side of the separator body.

4. The isothermal amplification nucleic acid reagent separator according to claim 3, characterized in that, A support block is fixedly installed on the side of the separator body; A retaining ring is detachably installed on the outer side of the separator body, above the support block.

5. The isothermal amplification nucleic acid reagent separator according to claim 1, characterized in that, A support column is fixedly installed below the support plate, and a support foot is fixedly installed below the support column.

6. The isothermal amplification nucleic acid reagent separator according to claim 1, characterized in that, The top of the support plate has a slot, and a square support column can be detachably installed inside the slot.

7. The isothermal amplification nucleic acid reagent separator according to claim 1, characterized in that, A motor assembly is installed at the bottom of the support plate; The motor assembly includes a rotary motor that is fixedly mounted on the bottom end of the support plate; A drive shaft is fixedly installed at the output end of the rotary motor, and a cross pin is fixedly installed at the end of the drive shaft.

8. The isothermal amplification nucleic acid reagent separator according to claim 1, characterized in that, A controller is fixedly installed on the top of the temperature control cover.

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