Integrated swab sample pretreatment immunochromatographic test card

Abstract

This utility model discloses an integrated swab sample pretreatment immunochromatographic detection card, including a card housing assembly and a test strip. The test strip is disposed in the inner cavity of the card housing assembly, which is equipped with a baffle assembly for placing the test strip. A stretching assembly for achieving negative pressure to accelerate chromatography is provided between the baffle assembly and the card housing assembly. Through the spiral groove, a vortex is automatically formed to wash the swab brush head when the swab is inserted and rotated, achieving full elution of the sample without manual stirring, reducing operation steps, and allowing non-professionals to operate in a standardized manner. This solves the problems of cumbersome operation and reliance on professional skills in the prior art. The vortex effect of the spiral groove reduces the risk of swab fiber shedding and avoids interference from impurities. The microporous filter membrane can effectively trap interfering substances and concentrate the target pathogen, solving the problems of sample contamination and concentration dilution leading to result deviation in the prior art.

Description

Technical Field

[0001] This utility model relates to the field of immunochromatographic detection card technology, and in particular to an integrated swab sample pretreatment immunochromatographic detection card. Background Technology

[0002] Immunochromatography, an analytical method that combines the principles of immunoreaction and chromatographic analysis, has been widely applied in key areas such as clinical diagnosis, environmental monitoring, and food safety due to its advantages of high specificity, ease of operation, and rapid detection. Its core principle utilizes colloidal gold or fluorescent microspheres as markers, achieving qualitative or semi-quantitative analysis of target analytes through band color development or fluorescence intensity. In existing test cards, the core component involved in the immunoreaction is the test strip (or a combination of the test strip and sample diluent). However, because the residence time of the sample on the sample pad is relatively short, the binding of the analyte to the marker (such as a fluorescent substance) is often insufficient, directly affecting the accuracy of the test results.

[0003] In existing technologies, such as the immunochromatographic detection card disclosed in announcement number CN208847748U, although the sample residue and overflow problems have been solved by optimizing the sample loading groove structure (concave arc surface, increased edge), the following significant limitations still exist in swab sample detection scenarios:

[0004] First, sample processing requires manually stirring the swab repeatedly in the elution solution to achieve elution, which is cumbersome and can easily cause swab fibers to fall off, interfering with the test results.

[0005] Second, the sample transfer process after elution can easily cause dilution, reduce the concentration of pathogens, and lead to a decrease in detection sensitivity;

[0006] Third, multi-step operations require a high degree of standardization from non-professionals, and improper operation can easily lead to deviations in results.

[0007] Therefore, there is an urgent need for an immunochromatographic assay card that can simplify the swab sample pretreatment process and improve detection efficiency and accuracy. Utility Model Content

[0008] The purpose of this invention is to provide an integrated swab sample pretreatment immunochromatographic detection card to solve the problems mentioned in the background art.

[0009] To achieve the above objectives, the main technical solutions adopted by this utility model include:

[0010] An integrated swab sample pretreatment immunochromatographic assay card includes a cartridge assembly and a test strip. The test strip is disposed within the inner cavity of the cartridge assembly. The inner cavity of the cartridge assembly has a baffle assembly for placing the test strip. A stretching assembly for achieving negative pressure to accelerate chromatography is provided between the baffle assembly and the cartridge assembly.

[0011] The casing assembly includes a lower shell and an upper cover, with the upper cover located on top of the lower shell;

[0012] The upper cover includes a shell cover that snaps onto the top of the lower shell. The shell cover has a sample application hole and an observation hole. The sample application hole is aligned with the sample application area of ​​the test strip, and the observation hole is aligned with the detection area of ​​the test strip. A microporous filter membrane is fixedly connected to the bottom of the shell cover. The microporous filter membrane is located at the bottom of the inner cavity of the sample application hole, and the inner cavity of the sample application hole has a spiral threaded groove.

[0013] As a preferred technical solution, the microporous filter membrane has a pore size of 0.5 μm and is made of a hydrophilic material.

[0014] As a preferred technical solution, the bottom of the inner cavity of the lower shell is integrally formed with a boss, the test strip is snapped onto the boss, and the test strip forms a gap with the top of the lower shell through the boss.

[0015] As a preferred technical solution, the partition assembly includes an inner shell placed inside the lower shell. The bottom of the inner cavity of the lower shell is integrally formed with an annular flange. The boss is located inside the annular flange. The inner shell is sealed and fastened to the top of the annular flange, forming a closed cavity with the lower shell. A flow channel is opened at the top of the inner shell and at the bottom of the microporous filter membrane. A slot is opened at the bottom of the inner cavity of the flow channel.

[0016] As a preferred technical solution, the top of the inner shell contacts the outer shell cover and is made of a transparent material.

[0017] As a preferred technical solution, the diameter of the groove is adapted to the width of the sample application area of ​​the test strip.

[0018] As a preferred technical solution, the stretching assembly includes a folded telescopic tube connected to the surface of the inner shell and away from the flow channel. The folded telescopic tube is located away from the flow channel, and a pull rod is fixedly connected to one end of the folded telescopic tube away from the inner shell. The pull rod extends movably through to the outside of the lower shell and is fixedly connected to a pull ring.

[0019] As a preferred technical solution, the pull rod has a cuboid structure, and the surface of the lower shell has a through hole for the pull rod to pass through, and the inner wall of the through hole is slidably connected to the surface of the pull rod.

[0020] As a preferred technical solution, both ends of the pull rod surface are integrally formed with retaining rings, and the diameter of the retaining rings is larger than the size of the through hole.

[0021] As a preferred technical solution, the gap and the folded telescopic tube form an airflow channel.

[0022] This utility model has at least the following beneficial effects:

[0023] This application utilizes a spiral groove to automatically create a vortex that washes the swab head during insertion and rotation, achieving thorough sample elution without manual agitation. This reduces operational steps and allows even non-professionals to operate the device correctly, solving the problems of cumbersome operation and reliance on professional skills in existing technologies. The vortex effect of the spiral groove reduces the risk of swab fiber shedding and avoids interference from impurities. The microporous filter membrane effectively traps interfering substances and concentrates target pathogens, solving the problems of sample contamination and concentration dilution leading to result deviations in existing technologies. The baffle and stretching components work synergistically: pulling the lever stretches the folded telescopic tube, creating an airflow channel through the gap between the test strip and the top of the lower shell. This generates negative pressure within the closed cavity, accelerating the chromatographic diffusion of the sample on the test strip and shortening the reaction time from the sample application area to the detection area, meeting the needs of rapid diagnosis and on-site testing. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the structure of this utility model;

[0025] Figure 2 This is an exploded view of the structure of this utility model;

[0026] Figure 3 This is an exploded view of the lower shell and partition assembly of this utility model.

[0027] In the diagram: 100, clamping assembly; 110, lower shell; 111, boss; 112, annular flange; 120, upper cover; 121, shell cover; 122, sample application port; 123, observation port; 124, microporous filter membrane; 125, spiral groove; 200, test strip; 300, partition assembly; 310, inner shell; 320, flow guide channel; 330, slot; 400, stretching assembly; 410, folding telescopic tube; 420, pull rod. Detailed Implementation

[0028] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0029] Please see Figures 1-3This utility model provides an integrated swab sample pretreatment immunochromatographic detection card, including a card housing assembly 100 and a test strip 200. The test strip 200 is disposed in the inner cavity of the card housing assembly 100. The inner cavity of the card housing assembly 100 is provided with a baffle assembly 300 for placing the test strip 200. A stretching assembly 400 for achieving negative pressure acceleration chromatography is provided between the baffle assembly 300 and the card housing assembly 100. The card housing assembly 100 includes a lower shell 110 and an upper cover 120, with the upper cover 120 disposed on the top of the lower shell 110. The upper cover 120 includes a shell cover 121 that fastens to the top of the lower shell 110. The shell cover 121 has a sample application hole 122 and an observation hole 123. The sample application hole 122 and the test strip 200 are connected by a slit hole 122 and an observation hole 123. The sample application area is aligned, and the observation hole 123 is aligned with the detection area of ​​the test strip 200. A microporous filter membrane 124 is fixedly connected to the bottom of the cover 121. The microporous filter membrane 124 is located at the bottom of the inner cavity of the sample application hole 122. The inner cavity of the sample application hole 122 has a spiral groove 125. The closed cavity design of the retaining assembly 100 provides a basis for negative pressure accelerated chromatography. The sample application hole 122 and the observation hole 123 are respectively aligned with the sample application area and the detection area of ​​the test strip 200 to ensure the accuracy of sample application and the observability of results. The microporous filter membrane 124 realizes sample filtration and preliminary concentration. The spiral groove 125 completes automatic swab elution through eddy current effect, reducing manual operation interference. The core effect is to integrate sample elution and filtration functions and simplify pretreatment.

[0030] Among them, the microporous filter membrane 124 has a pore size of 0.5μm and is made of hydrophilic material; the 0.5μm pore size can effectively trap swab fibers, particles and other interfering substances, and the hydrophilic material ensures that the sample is quickly wetted and penetrates into the test strip 200, avoiding sample retention and improving filtration efficiency and conduction speed.

[0031] The bottom of the inner cavity of the lower shell 110 is integrally formed with a boss 111, and the test strip 200 is snapped onto the boss 111. The test strip 200 forms a gap with the top of the lower shell 110 through the boss 111. The gap serves as part of the airflow channel, providing a working space for the negative pressure generated by the stretching component 400, ensuring that the negative pressure can effectively accelerate the sample chromatography.

[0032] The baffle assembly 300 includes an inner shell 310 placed inside the lower shell 110. An annular flange 112 is integrally formed at the bottom of the inner cavity of the lower shell 110. A boss 111 is located inside the annular flange 112. The inner shell 310 is sealed and fastened to the top of the annular flange 112, forming a closed cavity with the lower shell 110. A flow channel 320 is provided at the top of the inner shell 310 and at the bottom of the microporous filter membrane 124. A slot 330 is provided at the bottom of the inner cavity of the flow channel 320. The flow channel 320 receives the sample filtered by the microporous filter membrane 124, and the slot 330 accurately guides the sample to the sample application area of ​​the test strip 200 to ensure that the sample conduction is without deviation.

[0033] The top of the inner shell 310 contacts the cover 121 and is made of transparent material; the top contact enhances the sealing of the cavity and ensures stable negative pressure; the transparent material makes it easy to observe the detection area of ​​the test strip 200 inside the inner shell 310.

[0034] The diameter of the groove 330 is matched with the width of the sample application area of ​​the test strip 200; this ensures that the sample completely covers the sample application area of ​​200, avoiding sample waste or insufficient reaction due to partial non-contact, and improving the efficiency of the immune response.

[0035] The stretching assembly 400 includes a folded telescopic tube 410 connected to the surface of the inner shell 310 and away from the flow channel 320. The folded telescopic tube 410 is positioned away from the flow channel 320. A pull rod 420 is fixedly connected to one end of the folded telescopic tube 410 away from the inner shell 310. The pull rod 420 extends movably through to the outside of the lower shell 110 and is fixedly connected to a pull ring. Pulling the pull rod 420 stretches the folded telescopic tube 410, creating a negative pressure inside the inner shell 310. This accelerates the chromatographic diffusion of the sample on the test strip through the airflow channel, shortening the detection time.

[0036] The pull rod 420 has a cuboid structure, and the surface of the lower shell 110 has a through hole for the pull rod 420 to pass through. The inner wall of the through hole is slidably connected to the surface of the pull rod 420. The cuboid structure prevents the pull rod 420 from rotating and ensures the stability of the stretching direction. The sliding connection ensures that the pull rod 420 can be pulled smoothly and avoids jamming that affects the formation of negative pressure.

[0037] Both ends of the pull rod 420 are integrally formed with retaining rings, the diameter of which is larger than the size of the through hole; this limits the pulling stroke of the pull rod 420, prevents the pull rod 420 from falling out of the through hole, and ensures the structural stability of the tension assembly 400.

[0038] The gap and the folded telescopic tube 410 form an airflow channel; the channel provides a conduction path for negative pressure, so that the negative pressure generated by the stretching of the folded telescopic tube 410 can act on the test strip 200, accelerate the chromatographic diffusion of the sample, and ensure that the negative pressure acceleration function is effectively realized.

[0039] The working principle of this utility model is as follows:

[0040] Sample elution: Insert the collected swab into the sample application hole 122. The spiral groove 125 inside the sample application hole 122 contacts the swab brush head. When the swab is rotated, the spiral groove 125 guides the eluent or the sample liquid itself to form a vortex, which automatically washes the surface of the swab, so that the sample is fully separated from the swab and the elution is completed without manual stirring.

[0041] Sample filtration and conduction: The eluted sample is filtered through the microporous filter membrane 124 at the bottom of the sample application well 122 to remove impurities such as fibers and particles. The sample passing through the microporous filter membrane 124 flows into the guide channel 320 at the top of the inner shell 310 and is precisely dripped onto the sample application area of ​​the test strip 200 through the groove 330 at the bottom of the guide channel 320.

[0042] Negative pressure accelerated chromatography: Pulling the pull rod 420 by the pull ring causes the folded telescopic tube 410 to stretch, generating negative pressure in the closed cavity formed by the inner shell 310 and the lower shell 110; the negative pressure acts on the test strip 200 through the gap between the test strip 200 and the top of the lower shell 110, accelerating the chromatographic diffusion of the sample on the test strip 200 (moving from the sample application area to the detection area);

[0043] Results observation: After the sample undergoes an immune reaction with the marker in the detection area of ​​200, the colorimetric or fluorescence signal can be directly observed through the observation hole 123 of the top cover 120 to complete the detection.

[0044] All parts not described in this utility model are the same as or can be implemented using existing technology. Although embodiments of this utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of this utility model, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An integrated swab sample pretreatment immunochromatographic test card, characterized in that, The system includes a cartridge assembly (100) and a test strip (200). The test strip (200) is disposed within the inner cavity of the cartridge assembly (100). The inner cavity of the cartridge assembly (100) is provided with a baffle assembly (300) for placing the test strip (200). A stretching assembly (400) for achieving negative pressure accelerated chromatography is provided between the baffle assembly (300) and the cartridge assembly (100). The retaining assembly (100) includes a lower shell (110) and an upper cover (120), the upper cover (120) being disposed on the top of the lower shell (110); The upper cover (120) includes a shell cover (121) that is fastened to the top of the lower shell (110). The shell cover (121) has a sample application hole (122) and an observation hole (123). The sample application hole (122) is aligned with the sample application area of ​​the test strip (200), and the observation hole (123) is aligned with the detection area of ​​the test strip (200). A microporous filter membrane (124) is fixedly connected to the bottom of the shell cover (121). The microporous filter membrane (124) is located at the bottom of the inner cavity of the sample application hole (122). The inner cavity of the sample application hole (122) has a spiral threaded groove (125).

2. The integrated swab sample pretreatment immunochromatographic test card according to claim 1, characterized in that: The microporous filter membrane (124) has a pore size of 0.5 μm and is made of a hydrophilic material.

3. The integrated swab sample pretreatment immunochromatographic assay card according to claim 1, characterized in that: The bottom of the inner cavity of the lower shell (110) is integrally formed with a boss (111), the test strip (200) is snapped onto the boss (111), and the test strip (200) forms a gap with the top of the lower shell (110) through the boss (111).

4. The integrated swab sample pretreatment immunochromatographic assay card according to claim 3, characterized in that: The baffle assembly (300) includes an inner shell (310) placed inside the lower shell (110). The bottom of the inner cavity of the lower shell (110) is integrally formed with an annular flange (112). The boss (111) is located inside the annular flange (112). The inner shell (310) is sealed and fastened to the top of the annular flange (112) and forms a closed cavity with the lower shell (110). A flow channel (320) is opened at the top of the inner shell (310) and at the bottom of the microporous filter membrane (124). A slot (330) is opened at the bottom of the inner cavity of the flow channel (320).

5. The integrated swab sample pretreatment immunochromatographic assay card according to claim 4, characterized in that: The top of the inner shell (310) contacts the cover (121) and is made of a transparent material.

6. The integrated swab sample pretreatment immunochromatographic assay card according to claim 4, characterized in that: The diameter of the slot (330) is adapted to the width of the sample application area of ​​the test strip (200).

7. The integrated swab sample pretreatment immunochromatographic assay card according to claim 4, characterized in that: The stretching assembly (400) includes a folded telescopic tube (410) connected to the surface of the inner shell (310) and away from the flow channel (320). The folded telescopic tube (410) is disposed away from the flow channel (320). A pull rod (420) is fixedly connected to one end of the folded telescopic tube (410) away from the inner shell (310). The pull rod (420) extends movably through to the outside of the lower shell (110) and is fixedly connected to a pull ring.

8. The integrated swab sample pretreatment immunochromatographic detection card according to claim 7, characterized in that: The pull rod (420) has a cuboid structure, and the surface of the lower shell (110) is provided with a through hole for the pull rod (420) to pass through. The inner wall of the through hole is slidably connected to the surface of the pull rod (420).

9. The integrated swab sample pretreatment immunochromatographic assay card according to claim 8, characterized in that: Both ends of the pull rod (420) are integrally formed with retaining rings, and the diameter of the retaining rings is larger than the size of the through hole.

10. The integrated swab sample pretreatment immunochromatographic detection card according to claim 7, characterized in that: The gap and the folded telescopic tube (410) form an airflow channel.

Images