A centrifugal device for cell protein detection

Abstract

The utility model provides a centrifugal device for cell protein detection relates to cell protein detection field, including centrifugal unit, including the outer cover, the inner chamber of setting in the outer cover, the inner chamber of setting in the inner chamber of the cover that is connected in the outer cover top through the hinge activity, centrifugal assembly for carrying out centrifugal operation of setting in the inner chamber of the inner chamber of the inner chamber of setting, and the microcontroller of setting in the outer cover front, temperature control unit, including the base, the radiating fan of setting in the base inner chamber one side, the utility model discloses through temperature sensor real -time monitoring the temperature in the inner chamber cavity, and cooperate microcontroller can make the synergistic operation of semiconductor refrigerating sheet and radiating fan, can rapidly reduce and maintain the temperature in the inner chamber cavity, ensure that the protein sample keeps stable biological activity in the centrifugal process, through centrifugal assembly makes cell fragments, cell organ and so on solid particle and protein solution can more effectively separate, has improved the purity of protein.

Description

Technical Field

[0001] This invention belongs to the field of cell protein detection, specifically a centrifuge device for cell protein detection. Background Technology

[0002] Cellular protein detection is a crucial step in biological and medical research, playing a vital role in understanding cell function, disease mechanisms, and drug development. In this process, centrifugation devices play a critical role, effectively separating solid particles such as cell debris and organelles from protein solutions to obtain pure protein samples.

[0003] The working principle of existing centrifuge devices for cell protein detection is mainly based on centrifugation separation technology. When a centrifuge tube containing cell suspension or lysis buffer is placed in the centrifuge device and rotated at high speed, due to the centrifugal force, larger particles such as cell debris and organelles are thrown to the outer wall of the centrifuge tube and deposited, while smaller protein molecules remain in the supernatant to obtain the required protein sample.

[0004] However, when existing centrifuge devices operate at high speed for extended periods, the internal temperature of the centrifuge chamber is easily raised due to friction or motor heating. Because the chamber lacks effective temperature control, it is difficult to maintain a suitable temperature, which can easily lead to denaturation, degradation, or loss of biological activity of protein samples during centrifugation, greatly affecting subsequent detection results and analysis.

[0005] In summary, this invention provides a centrifugation device for cell protein detection to solve the above-mentioned problems. Utility Model Content

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution:

[0007] A centrifuge device for detecting cellular proteins, comprising:

[0008] The centrifuge unit includes an outer shell, an inner liner disposed in the inner cavity of the outer shell, a cover movably connected to the top of the outer shell via a hinge, a centrifuge assembly disposed in the inner cavity of the inner liner for centrifugation operations, and a microcontroller disposed on the front of the outer shell.

[0009] The temperature control unit includes a base, a cooling fan disposed on one side of the inner cavity of the base, a semiconductor cooling chip disposed at the bottom of the inner cavity of the inner liner for heat exchange and cooling of the inner liner, a temperature sensor disposed in the inner cavity of the inner liner, and ventilation slots opened on both sides of the base.

[0010] Furthermore, in this utility model, the outer shell is connected to the base, the bottom of the semiconductor cooling chip penetrates the inner liner and extends into the inner cavity of the outer shell, and an intercepting net is fixedly connected to the inner cavity of the ventilation slot.

[0011] Furthermore, in this invention, the microcontroller has a display screen and control buttons on its front side, the output terminal of the microcontroller is connected to the input terminal of the display screen, the output terminal of the temperature sensor is connected to the input terminal of the microcontroller, and the output terminal of the microcontroller is connected to the input terminals of the semiconductor cooling chip and the cooling fan, respectively.

[0012] Furthermore, in this utility model, the centrifugal assembly includes a geared motor, a transmission rod connected to the output shaft of the geared motor, a rotor disk fixedly connected to the top of the transmission rod, and a tube groove disposed on the top of the rotor disk. The bottom of the geared motor is fixedly connected to the bottom of the inner cavity of the base, and the input end of the geared motor is connected to the output end of the microcontroller.

[0013] Furthermore, in this utility model, a sealing ring is fixedly connected to the bottom of the cap, the sealing ring extends into the inner cavity of the inner liner and contacts the inner wall of the inner liner, and a viewing window is provided on the top of the cap.

[0014] Beneficial effects: This utility model has the following beneficial effects:

[0015] This invention uses a temperature sensor to monitor the temperature inside the inner chamber in real time. In conjunction with a microcontroller, the semiconductor cooling chip and the cooling fan work together to rapidly reduce and maintain the temperature inside the inner chamber, ensuring that the protein sample maintains stable biological activity during centrifugation. The centrifugation assembly enables more effective separation of solid particles such as cell debris and organelles from the protein solution, thereby improving the purity of the protein. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the cross-sectional structure of the inner liner of this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the cover of this utility model from a downward view;

[0019] Figure 4 This is a schematic diagram of the base structure of this utility model;

[0020] Figure 5 This is a schematic diagram of the system principle of this utility model.

[0021] In the picture:

[0022] 100. Centrifuge unit; 110. Outer casing; 120. Cover; 121. Sealing ring; 122. Viewing window; 130. Inner liner; 140. Centrifuge assembly; 141. Gear motor; 142. Transmission rod; 143. Rotor disc; 144. Tube groove; 150. Microcontroller; 200. Temperature control unit; 210. Base; 220. Cooling fan; 230. Semiconductor cooling chip; 240. Temperature sensor; 250. Ventilation slot. Detailed Implementation

[0023] To better understand the technical content of this utility model, specific embodiments are described below in conjunction with the accompanying drawings. Various aspects of this utility model are described in this disclosure with reference to the accompanying drawings, which illustrate numerous illustrative embodiments. The embodiments of this disclosure are not necessarily defined to include all aspects of this utility model. It should be understood that the various concepts and embodiments described above, as well as those described in more detail below, can be implemented in any of many ways, because the concepts and embodiments disclosed in this utility model are not limited to any particular implementation. Furthermore, some aspects of this utility model can be used alone or in any suitable combination with other aspects disclosed in this utility model.

[0024] Example 1

[0025] like Figure 1-5 As shown, this is the first embodiment of the present invention, which provides a centrifuge device for detecting cell proteins, including...

[0026] The centrifuge unit 100 includes an outer shell 110, an inner liner 130 disposed in the inner cavity of the outer shell 110, a cover 120 movably connected to the top of the outer shell 110 via a hinge, a centrifuge assembly 140 disposed in the inner cavity of the inner liner 130 and used for centrifugation operation, and a microcontroller 150 disposed on the front of the outer shell 110.

[0027] The temperature control unit 200 includes a base 210, a cooling fan 220 disposed on one side of the inner cavity of the base 210, a semiconductor cooling chip 230 disposed at the bottom of the inner cavity of the inner liner 130 and used for heat exchange and cooling of the inner cavity of the inner liner 130, a temperature sensor 240 disposed in the inner cavity of the inner liner 130, and ventilation slots 250 opened on both sides of the base 210.

[0028] like Figure 1-5As shown, the inner liner 130 provides space for centrifugation, and the cap 120 can seal the inner liner 130 to ensure its airtightness. The centrifugation assembly 140 allows centrifuge tubes containing cell suspension or lysis buffer to be centrifuged under centrifugal force, causing larger particles such as cell debris and organelles to be thrown to the outer wall of the centrifuge tube and deposited, while smaller protein molecules remain in the supernatant. This achieves centrifugation. The temperature sensor 240 monitors the temperature inside the inner liner 130 in real time and feeds the data back to the microcontroller 150. The microcontroller 150 determines whether the internal temperature is at a suitable level and can control the semiconductor cooling chip 230 to start, cooling the inner liner 130. At the same time, the cooling fan 220, together with the ventilation slot 250, can accelerate heat dissipation, which can quickly reduce and maintain the temperature inside the inner liner 130, achieving temperature control during centrifugation and ensuring that the protein sample maintains stable biological activity during centrifugation.

[0029] Example 2

[0030] Reference Figure 1 , 4 5, is the second embodiment of this utility model, which is based on the previous embodiment.

[0031] In this embodiment, the outer casing 110 is connected to the base 210, the bottom of the semiconductor cooling chip 230 penetrates the inner liner 130 and extends into the inner cavity of the outer casing 110, and an intercepting net is fixedly connected to the inner cavity of the ventilation slot 250.

[0032] The microcontroller 150 has a display screen and control buttons on its front. The output of the microcontroller 150 is connected to the input of the display screen. The output of the temperature sensor 240 is connected to the input of the microcontroller 150. The output of the microcontroller 150 is connected to the input of the thermoelectric cooler 230 and the cooling fan 220 respectively. The temperature sensor 240 is a DS18B20, the thermoelectric cooler 230 is a TEC1-12706, and the microcontroller 150 is an ESP32 series microcontroller.

[0033] like Figure 1 , 4As shown in Figure 5, the outer casing 110 is connected to the base 210, providing an effective heat dissipation channel. The thermoelectric cooler 230 extends through the bottom of the inner liner 130 and into the inner cavity of the outer casing 110. This arrangement allows the thermoelectric cooler 230 to directly cool the inner cavity of the inner liner 130, improving the efficiency and accuracy of temperature control. The control buttons on the front of the microcontroller 150 allow users to easily set centrifugation parameters such as speed, time, or temperature, and the centrifugation process can be started via the control buttons. The microcontroller 150 is connected to the temperature sensor 240 and the thermoelectric cooler 230, respectively. The device is connected to the cooling fan 220. During use, the temperature sensor 240 monitors the temperature inside the inner chamber 130 in real time and sends the data to the microcontroller 150. The microcontroller 150 controls the working status of the semiconductor cooling chip 230 and the cooling fan 220 according to the set temperature range and the actual temperature value. When the temperature exceeds the set value, the semiconductor cooling chip 230 starts cooling to reduce the temperature inside the inner chamber 130. At the same time, the cooling fan 220 accelerates its rotation to expel heat from the device, effectively ensuring that the centrifugation temperature is always kept within a suitable range and avoiding the impact of high temperature on protein samples.

[0034] Example 3

[0035] Reference Figure 2-5 This is the third embodiment of the present invention, which is based on the first two embodiments.

[0036] In this embodiment, the centrifugal assembly 140 includes a geared motor 141, a transmission rod 142 connected to the output shaft of the geared motor 141, a rotor disk 143 fixedly connected to the top of the transmission rod 142, and a tube groove 144 disposed on the top of the rotor disk 143. The bottom of the geared motor 141 is fixedly connected to the bottom of the inner cavity of the base 210, and the input end of the geared motor 141 is connected to the output end of the microcontroller 150.

[0037] A sealing ring 121 is fixedly connected to the bottom of the cover 120. The sealing ring 121 extends into the inner cavity of the inner liner 130 and contacts the inner wall of the inner liner 130. A viewing window 122 is provided on the top of the cover 120.

[0038] like Figure 2-5As shown, the tube groove 144 is used to place centrifuge tubes containing cell suspension or lysis buffer. The output shaft of the geared motor 141 transmits power through the transmission rod 142. The transmission rod 142 drives the rotor disk 143 and the centrifuge tubes containing cell suspension or lysis buffer in the tube groove 144 to rotate. Under the action of centrifugal force, larger particles such as cell debris and organelles are thrown to the outer wall of the centrifuge tube and deposited, while smaller protein molecules remain in the supernatant. This completes the centrifugation operation. The sealing ring 121 extends into the inner cavity of the inner liner 130 and contacts the inner wall of the inner liner 130 to form a tight sealing structure, which effectively prevents the internal temperature of the inner liner 130 from being affected by the external environment. The viewing window 122 is opened on the top of the cap 120, allowing the user to observe the centrifugation status and sample condition in real time during the centrifugation process.

[0039] In use, first place the centrifuge tubes containing cell suspension or lysis buffer into the tube groove 144 of the rotor disk 143, and then cover them with the cap 120. The microcontroller 150 then controls the geared motor 141 to start, which drives the rotor disk 143 to rotate at high speed via the transmission rod 142. The rotor disk 143 drives the centrifuge tubes containing cell suspension or lysis buffer in the tube groove 144 to rotate. Under the action of centrifugal force, larger particles such as cell debris and organelles are thrown against the outer wall of the centrifuge tube and deposited, while smaller protein molecules remain in the supernatant. This achieves centrifugation. During centrifugation, the temperature... Temperature sensor 240 monitors the temperature inside the inner chamber 130 in real time and feeds the data back to microcontroller 150. Microcontroller 150 analyzes the temperature data and, when the temperature exceeds the set value, controls the semiconductor cooling chip 230 and cooling fan 220 to start, thereby cooling the inner chamber 130. At the same time, the cooling fan 220, together with the ventilation slot 250, can accelerate heat dissipation, which can quickly reduce and maintain the temperature inside the inner chamber 130, realizing temperature control during centrifugation and effectively ensuring that the protein sample maintains stable biological activity during centrifugation.

[0040] All standard parts used in this application can be purchased from the market, and can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art. The control method is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art and is common knowledge in the field. Since this application is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail in this application.

[0041] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Those skilled in the art to which this invention pertains can make various modifications and refinements without departing from the spirit and scope of the present invention. Therefore, the scope of protection of this invention shall be determined by the claims.

Claims

1. A centrifuge device for detecting cellular proteins, characterized in that: include The centrifuge unit (100) includes an outer shell (110), an inner liner (130) disposed in the inner cavity of the outer shell (110), a cover (120) movably connected to the top of the outer shell (110) by a hinge, a centrifuge assembly (140) disposed in the inner cavity of the inner liner (130) and used for centrifugation, and a microcontroller (150) disposed on the front of the outer shell (110). The temperature control unit (200) includes a base (210), a cooling fan (220) disposed on one side of the inner cavity of the base (210), a semiconductor cooling chip (230) disposed at the bottom of the inner cavity of the inner liner (130) and used for heat exchange and cooling of the inner cavity of the inner liner (130), a temperature sensor (240) disposed in the inner cavity of the inner liner (130), and ventilation slots (250) opened on both sides of the base (210).

2. The centrifuge device for cell protein detection as described in claim 1, characterized in that: The outer casing (110) is connected to the base (210), the bottom of the semiconductor cooling chip (230) penetrates the inner liner (130) and extends into the inner cavity of the outer casing (110), and an intercepting net is fixedly connected to the inner cavity of the ventilation slot (250).

3. The centrifuge device for cell protein detection as described in claim 1, characterized in that: The microcontroller (150) has a display screen and control buttons on its front side. The output terminal of the microcontroller (150) is connected to the input terminal of the display screen. The output terminal of the temperature sensor (240) is connected to the input terminal of the microcontroller (150). The output terminal of the microcontroller (150) is connected to the input terminals of the thermoelectric cooler (230) and the cooling fan (220) respectively.

4. The centrifuge device for cell protein detection as described in claim 1, characterized in that: The centrifugal assembly (140) includes a geared motor (141), a transmission rod (142) connected to the output shaft of the geared motor (141), a rotor disk (143) fixedly connected to the top of the transmission rod (142), and a tube groove (144) disposed on the top of the rotor disk (143). The bottom of the geared motor (141) is fixedly connected to the bottom of the inner cavity of the base (210), and the input end of the geared motor (141) is connected to the output end of the microcontroller (150).

5. The centrifuge device for cell protein detection as described in claim 1, characterized in that: A sealing ring (121) is fixedly connected to the bottom of the cover (120). The sealing ring (121) extends into the inner cavity of the inner liner (130) and contacts the inner wall of the inner liner (130). A viewing window (122) is provided on the top of the cover (120).

Images