Sample detection cartridge and sample detection apparatus

Abstract

The utility model discloses a sample detection box and sample detection equipment, sample detection box includes liquid storage subassembly and transfer subassembly, and liquid storage subassembly includes liquid storage box and magnetic bead, is set up with a plurality of pretreatment groove on the liquid storage box, and the magnetic bead is located in one of the pretreatment groove, transfer subassembly includes cover and partition cap, and the cover includes shell body and transfer sleeve, and shell body rotatably is located on the liquid storage box, and transfer sleeve sets up through shell body and can rotate to correspond any one pretreatment groove setting, and the first end of transfer sleeve is used for the magnetic bar of sample detection equipment to extend into and adsorb the magnetic bead, and the partition cap is located at the second end of transfer sleeve and blocks the transfer sleeve. The partition cap prevents the magnetic bead from contacting the outside world, and the sample detection box transfers and purifies nucleic acid by adsorbing nucleic acid, which omits the operation step of removing waste liquid and greatly improves the accuracy of sample detection.

Description

Technical Field

[0001] This utility model belongs to the field of sample detection technology, specifically relating to a sample detection box and sample detection equipment. Background Technology

[0002] Before testing a sample, it needs to be pretreated to extract and purify the nucleic acid in the sample. Currently, the sample pretreatment process usually involves using pipette tips to transfer the sample and reagents and remove waste liquid. During the removal of waste liquid, waste liquid may remain in the sample container and the flow channel of the pipette tip. The inhibitors in the waste liquid mixed with the purified nucleic acid can easily lead to false positive results in the sample test, reducing the accuracy of the sample test. Utility Model Content

[0003] In view of the above-mentioned defects or deficiencies, this utility model provides a sample detection box and sample detection device, aiming to solve the technical problem of low sample detection accuracy.

[0004] To achieve the above objectives, this utility model provides a sample detection box, which includes:

[0005] The liquid storage assembly includes a liquid storage box and a magnetic bead. The liquid storage box has multiple pretreatment slots, and the magnetic bead is located in one of the pretreatment slots.

[0006] The transfer assembly includes a housing and a separator cap. The housing includes a housing body and a transfer sleeve. The housing body is rotatably mounted on the liquid storage box. The transfer sleeve passes through the housing body and can be rotated to be positioned in any corresponding pretreatment tank. The first end of the transfer sleeve is used for the magnetic rod of the sample detection device to extend into and attract magnetic beads. The separator cap is located at the second end of the transfer sleeve and seals the transfer sleeve. The separator cap is used to separate the magnetic rod and magnetic beads.

[0007] In this embodiment of the utility model, the separator cap includes a sleeve portion and an elastic pushing portion. The sleeve portion is sleeved on the second end of the transfer sleeve, and the elastic pushing portion is disposed at one end of the sleeve portion and blocks the transfer sleeve. The first end of the transfer sleeve is also used for the mixing sleeve of the sample detection device to extend into and push the elastic pushing portion.

[0008] In this embodiment of the utility model, the liquid storage box includes a box body and a detection tube. The box body has multiple pretreatment grooves. The detection tube passes through the box body and includes a tube body and an injection part. The tube body has a liquid storage cavity. The injection part is located on one side of the tube body and has an injection groove. The side wall of the injection groove has a communication port that communicates with the liquid storage cavity. The liquid storage assembly also includes a sealing plug. The sealing plug is movably disposed in the liquid storage cavity and is used to block the communication port.

[0009] In this embodiment of the utility model, a flow guiding slope is provided on the bottom wall of the injection tank, and the flow guiding slope is inclined from top to bottom in the direction towards the communication port.

[0010] In this embodiment of the utility model, the transfer assembly further includes a push plunger, and a guide hole is also provided on the shell body. The guide hole can be rotated to communicate with the liquid storage chamber. The push plunger is movably disposed in the guide hole and is used to push the sealing plug to block the communication port.

[0011] In this embodiment of the utility model, the box body is also provided with a placement groove, the detection tube, the placement groove and a plurality of pretreatment grooves are arranged circumferentially at intervals, the shell body is provided with a pipetting hole, the pipetting hole can be rotated to communicate with the injection groove, the placement groove or any one of the pretreatment grooves, and the transfer component also includes a pipetting tip, which passes through the pipetting hole and extends into the placement groove.

[0012] In this embodiment of the utility model, a detection port is provided on the box body, and the number of detection tubes is set to multiple, with the multiple detection tubes arranged at intervals and each corresponding to a detection port.

[0013] In this embodiment of the utility model, a sample feeding hole is provided on the shell body, and multiple pretreatment tanks include a sample tank, an elution tank and at least one washing tank. The sample feeding hole can be rotated to communicate with the sample tank. The transfer assembly also includes a sealing cap covering the sample feeding hole.

[0014] In this embodiment of the utility model, the shell body includes a top plate, a surrounding plate, and a rotating column. The surrounding plate surrounds the periphery of the top plate, the transfer sleeve passes through the top plate, one end of the rotating column is connected to the top plate, and the other end of the rotating column rotatably passes through the liquid storage box.

[0015] And / or, the second end of the transfer sleeve is provided with an installation step, and the separator cap is fitted onto the installation step.

[0016] To achieve the above objectives, the present invention also provides a sample detection device, which includes a sample detection box as described above.

[0017] Through the above technical solutions, the sample detection box and sample detection device provided by this utility model embodiment have the following beneficial effects:

[0018] In the technical solution of this utility model, the shell body drives the transfer sleeve to rotate to the position of the pretreatment tank containing the magnetic beads. The magnetic rod extends from the first end of the transfer sleeve into the transfer sleeve to attract the magnetic beads to the separator cap. This allows the shell body to drive the magnetic beads to rotate to the position of any corresponding pretreatment tank through the transfer sleeve and the separator cap. When the magnetic rod retracts out of the transfer sleeve, the magnetic beads are released from the magnetic rod and fall into the corresponding pretreatment tank, thus realizing the transfer of magnetic beads between multiple pretreatment tanks. The transfer sleeve transfers the magnetic beads separately. Samples are pretreated in different pretreatment tanks for nucleic acid extraction, lysis, washing, or elution to obtain purified nucleic acids. The magnetic beads remain within the shell throughout the purification process, and the separator cap effectively prevents contact between the magnetic beads and the external environment, thus preventing external impurities from contaminating the purified nucleic acids. Furthermore, the sample detection kit transfers and purifies nucleic acids by adsorbing them onto the magnetic beads, eliminating the need for waste liquid removal and effectively preventing mixing of waste liquid with the purified nucleic acids, significantly improving the accuracy of sample detection.

[0019] Other features and advantages of this invention will be described in detail in the following detailed description section. Attached Figure Description

[0020] The accompanying drawings are provided to further illustrate the embodiments of the present invention and form part of the specification. They are used together with the following detailed description to explain the embodiments of the present invention, but do not constitute a limitation thereof. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0021] Figure 1 This is a schematic diagram of the assembly structure of a sample detection box according to an embodiment of the present invention;

[0022] Figure 2 This is an exploded structural diagram of a sample detection box according to an embodiment of the present invention;

[0023] Figure 3 This is a cross-sectional structural schematic diagram of a sample detection box according to an embodiment of the present invention;

[0024] Figure 4 This is a schematic diagram of the structure of the transfer component in a sample detection box according to an embodiment of the present invention;

[0025] Figure 5 This is a schematic diagram of the liquid storage component in a sample detection box according to an embodiment of the present invention;

[0026] Figure 6 This is a cross-sectional view of the liquid storage box in a liquid storage assembly according to an embodiment of the present invention from one perspective;

[0027] Figure 7 This is a cross-sectional view of the liquid storage box in a liquid storage assembly according to an embodiment of the present invention from another perspective.

[0028] Explanation of reference numerals in the attached figures

[0029] Detailed Implementation

[0030] The specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of this utility model.

[0031] The sample testing kit of this utility model is described below with reference to the accompanying drawings.

[0032] like Figures 1 to 4 As shown, this utility model provides a sample testing kit, which includes a liquid storage component 10 and a transfer component 20. The liquid storage component 10 includes a liquid storage box 11 and a magnetic bead 12. The liquid storage box 11 has multiple pretreatment slots 111, and the magnetic bead 12 is disposed in one of the pretreatment slots 111. The transfer component 20 includes a cover 21 and a separating cap 22. The cover 21 includes a shell body 211 and a transfer sleeve 212. The shell body 211 is rotatably disposed on the liquid storage box 11. The transfer sleeve 212 passes through the shell body 211 and can be rotated to be disposed in any one of the pretreatment slots 111. The first end of the transfer sleeve 212 is used for the magnetic rod 200 of the sample testing device to extend into and attract the magnetic bead 12. The separating cap 22 is disposed at the second end of the transfer sleeve 212 and seals the transfer sleeve 212. The separating cap 22 is used to separate the magnetic rod 200 and the magnetic bead 12.

[0033] It should be noted that the sample detection kit of this utility model can be applied in sample detection equipment to preprocess samples, extract and purify nucleic acids, and detect the purified nucleic acids. Sample preprocessing and detection can be performed by placing the collected sample solution into the sample detection kit and installing the sample detection kit into the sample detection equipment. The sample detection equipment includes a magnetic rod 200 and a mixing sleeve 300. The magnetic rod 200 and the mixing sleeve 300 can be raised and lowered relative to the sample detection kit, and both can rotate relative to the sample detection kit. The magnetic rod 200 is used to adsorb magnetic beads 12, and the mixing sleeve 300 is used for the magnetic rod 200 to pass through.

[0034] Specifically, the storage box 11 has multiple pretreatment tanks 111, which are used to hold sample processing solutions such as lysis buffer, washing buffer, and elution buffer, as well as sample solutions. One of the pretreatment tanks 111 contains magnetic beads 12, which are used to adsorb nucleic acids. The shell body 211 is disposed on the storage box 11 and can rotate relative to the storage box 11. The transfer sleeve 212 is disposed on the shell body 211, so that the shell body 211 can drive the transfer sleeve 212 to rotate and rotate the transfer sleeve 212 to a position corresponding to any one of the pretreatment tanks 111. The end of the transfer sleeve 212 away from the storage box 11 is designated as the first end of the transfer sleeve 212, and the end of the transfer sleeve 212 facing the storage box 11 is designated as the second end of the transfer sleeve 212. The second end of the transfer sleeve 212 is fitted with a separator cap 22.

[0035] During sample testing, the shell body 211 drives the transfer sleeve 212 to rotate to the position of the pretreatment tank 111 containing the magnetic beads 12. The magnetic rod 200 extends from the first end of the transfer sleeve 212 into the transfer sleeve 212 to attract the magnetic beads 12 onto the separator cap 22. This allows the shell body 211 to drive the magnetic beads 12 to rotate to the position of any one of the pretreatment tanks 111 via the transfer sleeve 212 and the separator cap 22. As the magnetic rod 200 retracts out of the transfer sleeve 212, the magnetic beads 12 are released from the magnetic rod 200 and fall into the corresponding pretreatment tank 111, thus realizing the transfer of the magnetic beads 12 between multiple pretreatment tanks 111. The transfer sleeve 212 transfers magnetic beads 12 to different pretreatment tanks 111 for sample pretreatment such as nucleic acid extraction, lysis, washing, or elution to obtain purified nucleic acid. During the nucleic acid purification process, the magnetic beads 12 remain inside the shell body 211, and the separator cap 22 effectively prevents the magnetic beads 12 from contacting the outside world by blocking the magnetic beads 12 and the magnetic rod 200, thereby preventing external impurities from mixing into the purified nucleic acid. Furthermore, the sample detection kit transfers and purifies nucleic acid by adsorbing it with the magnetic beads 12, eliminating the need for waste liquid removal and effectively preventing the waste liquid from mixing with the purified nucleic acid, thus significantly improving the accuracy of sample detection.

[0036] In this embodiment of the utility model, the separator cap 22 includes a sleeve portion 221 and an elastic pushing portion 222. The sleeve portion 221 is sleeved on the second end of the transfer sleeve 212, and the elastic pushing portion 222 is disposed at one end of the sleeve portion 221 and blocks the transfer sleeve 212. The first end of the transfer sleeve 212 is also used for the mixing sleeve 300 of the sample detection device to extend into and push the elastic pushing portion 222.

[0037] like Figures 2 to 4As shown, one end of the sleeve 221 is provided with an elastic pushing part 222. The sleeve 221 is sleeved on the second end of the transfer sleeve 212 so that the elastic pushing part 222 blocks the transfer sleeve 212. The elastic pushing part 222 can block the magnetic rod 200 and the magnetic bead 12 when the magnetic rod 200 attracts the magnetic bead 12, effectively preventing the magnetic bead 12 from contacting the outside world and preventing external impurities from entering the shell body 211, thus improving the accuracy of sample detection. In addition, the mixing sleeve 300 can extend from the first end of the transfer sleeve 212. The sample is inserted into the transfer sleeve 212 and moved up and down within the transfer sleeve 212. During the up and down movement, the mixing sleeve 300 pushes the elastic pushing part 222, causing the elastic pushing part 222 to repeatedly tap the liquid in the pretreatment tank 111. This achieves the mixing of the sample liquid with the sample processing liquid, and the nucleic acid adsorbed on the magnetic beads 12 with the sample processing liquid, improving the mixing uniformity. Furthermore, the elastic pushing part 222 seals the transfer sleeve 212, preventing external impurities from entering the shell body 211 during the mixing process, further improving the accuracy of sample detection.

[0038] Furthermore, the second end of the transfer sleeve 212 is provided with an installation step 2121, and the separator cap 22 is fitted onto the installation step 2121. For example... Figure 3 and Figure 4 As shown, the sleeve 221 is fitted onto the mounting step 2121 and sealed to the transfer sleeve 212. The mounting step 2121 is used for positioning and installing the sleeve 221, which is convenient and improves the ease of assembly. Furthermore, the separator cap 22 is made of an integrally formed elastic material, which not only improves the sealing performance of the separator cap 22 and effectively prevents external impurities from entering the shell body 211, but also allows the mixing sleeve 300 to push the elastic pushing part 222. Under the pushing action of the mixing sleeve 300, the elastic pushing part 222 elastically stretches and strikes the liquid in the pretreatment tank 111 for mixing, further improving the mixing uniformity.

[0039] In this embodiment of the utility model, the liquid storage box 11 includes a box body 112 and a detection tube 113. The box body 112 is provided with a plurality of pretreatment grooves 111. The detection tube 113 passes through the box body 112 and is provided with a tube body portion 1131 and a liquid injection portion 1133. A liquid storage cavity 1132 is provided in the tube body portion 1131. The liquid injection portion 1133 is provided on one side of the tube body portion 1131 and a liquid injection groove 1134 is provided in the liquid injection portion 1133. A communication port 1135 communicating with the liquid storage cavity 1132 is provided on the side wall of the liquid injection groove 1134. The liquid storage assembly 10 also includes a sealing plug 13. The sealing plug 13 is movably provided in the liquid storage cavity 1132 and is used to block the communication port 1135.

[0040] like Figure 6 and Figure 7As shown, during sample testing, the purified liquid is injected into the injection tank 1134, allowing the purified liquid to flow through the connecting port 1135 into the storage chamber 1132 of the tube body 1131. The storage chamber 1132 contains the test reagent or the lyophilized bulb for testing. After the injection is completed, the sealing plug 13 is pushed into the storage chamber 1132 to block the connecting port 1135 and thus seal the storage chamber 1132. This effectively prevents impurities or inhibitors from entering the storage chamber 1132 and causing contamination, further improving the accuracy and reliability of the sample test results.

[0041] Furthermore, such as Figure 7 As shown, the bottom wall of the injection tank 1134 is provided with a flow guiding slope 1136. The flow guiding slope 1136 is inclined from top to bottom in the direction towards the connecting port 1135. The flow guiding slope 1136 plays the role of guiding the flow of liquid, so that the liquid injected into the injection tank 1134 can flow quickly into the storage chamber 1132 along the inclined direction of the flow guiding slope 1136, effectively preventing liquid residue in the injection tank 1134 and improving the detection accuracy and detection efficiency.

[0042] In this embodiment of the utility model, the transfer component 20 further includes a push plunger 24, and a guide hole 2112 is also provided on the shell body 211. The guide hole 2112 can be rotated to communicate with the liquid storage chamber 1132. The push plunger 24 is movably disposed in the guide hole 2112 and is used to push the sealing plug 13 to block the communication port 1135.

[0043] like Figures 1 to 5 As shown, when the processed liquid is injected into the injection tank 1134, the shell body 211 can drive the push plunger 24 to rotate to the position of the corresponding tube body 1131, so that the push plunger 24 is above the sealing plug 13. By pushing the push plunger 24, the push plunger 24 moves downward along the guide hole 2112 and pushes the sealing plug 13, so that the sealing plug 13 moves toward the liquid storage chamber 1132 and blocks the communication port 1135. By setting the push plunger 24 to push the sealing plug 13 to block the communication port 1135, the sealing convenience of the liquid storage chamber 1132 is improved.

[0044] In this embodiment of the utility model, the box body 112 is also provided with a placement groove 1121, the detection tube 113, the placement groove 1121 and a plurality of pretreatment grooves 111 are arranged circumferentially at intervals, the shell body 211 is provided with a pipetting hole 2111, the pipetting hole 2111 can be rotated to communicate with the injection groove 1134, the placement groove 1121 or any one of the pretreatment grooves 111, and the transfer assembly 20 also includes a pipetting tip 23, which passes through the pipetting hole 2111 and extends into the placement groove 1121.

[0045] like Figures 2 to 5As shown, during sample testing, the sample testing device can pick up the pipette tip 23 in the pipette orifice 2111 and drive the pipette tip 23 to rise outside the placement tank 1121, thereby allowing the shell body 211 to rotate relative to the liquid storage box 11. The placement tank 1121 serves to accommodate the pipette tip 23, improving the structural integration of the sample testing box. Furthermore, the shell body 211 can drive the pipette tip 23 in the pipette orifice 2111 to rotate to the position of the corresponding detection tube 113 or any pretreatment tank 111, so that the pipette tip 23 can pick up the pretreated liquid and transfer it into the detection tube 113. The detection tube 113 is used to hold the detection reagent or the lyophilized bulb for detection. After pretreatment... The liquid can be mixed with the detection reagent or lyophilized beads for detection for testing. The entire process of sample pretreatment and detection is sealed within the shell body 211, effectively preventing external impurities from entering and causing contamination. This realizes the detection method of sample in and result out, further improving the accuracy and reliability of sample detection results. In addition, the sample detection kit extracts and purifies nucleic acids by transferring magnetic beads 12. The pipette tip 23 only needs to pick up and transfer the liquid after processing, eliminating the waste liquid removal step and effectively preventing waste liquid residue in the pipette tip 23. This prevents inhibitors in the waste liquid from mixing with the purified nucleic acid and causing false positives in the detection results, greatly improving the accuracy of sample detection results.

[0046] Furthermore, such as Figure 6 and Figure 7 As shown, the shell body 211 drives the pipette tip 23 to rotate to the position corresponding to the pretreatment tank 111, so that the pipette tip 23 can pick up the processed liquid. Then, the shell body 211 drives the pipette tip 23 to rotate to the position corresponding to the injection section 1133, so that the pipette tip 23 can inject the processed liquid into the injection tank 1134 and flow into the liquid storage chamber 1132 of the tube body 1131 through the connecting port 1135. The liquid storage chamber 1132 contains the test reagent or the lyophilized bulb for testing. After the liquid injection is completed, the sealing plug 13 is pushed into the liquid storage chamber 1132, so that the sealing plug 13 blocks the connecting port 1135 and seals the liquid storage chamber 1132, effectively preventing impurities or inhibitors from entering the liquid storage chamber 1132 and causing contamination, and further improving the accuracy and reliability of the sample test results.

[0047] In this embodiment of the utility model, a detection port 1122 is provided on the box body 112, and the number of detection tubes 113 is set to multiple, with the multiple detection tubes 113 arranged at intervals and each corresponding to the detection port 1122. Figure 1 and Figure 2As shown, the number of sealing plugs 13, guide holes 2112, and push plungers 24 are all consistent with the number of detection tubes 113 and are set one-to-one. Detection ports 1122 are opened on the box body 112 at the positions corresponding to multiple detection tubes 113. The detection ports 1122 are set for the PCR detection mechanism of the sample detection equipment, so that the PCR detection mechanism can amplify and optically detect the purified nucleic acid in the detection tubes 113. During the nucleic acid extraction and purification, liquid transfer, and sample detection process, the sample solution, sample processing solution, and nucleic acid adsorbed on the magnetic beads 12 are always sealed inside the shell body 211, eliminating manual operation and waste liquid removal steps throughout the process. This helps to reduce the contamination of inhibitors and external impurities, realizes sample entry and result exit, and greatly improves the accuracy of sample detection results. The sample detection box has the advantages of small size and easy portability. In addition, different types of detection reagents or lyophilized beads for detection can be contained in the multiple detection tubes 113 respectively, realizing the simultaneous detection of multiple targets, improving the efficiency of sample detection, and realizing high-throughput sample detection.

[0048] In this embodiment of the present invention, the shell body 211 is provided with a sample feeding hole 2113, and a plurality of pretreatment tanks 111 include a sample tank 111a, an elution tank 111b and at least one washing tank 111c. The sample feeding hole 2113 can be rotated to communicate with the sample tank 111a. The transfer assembly 20 also includes a sealing cap 25 covering the sample feeding hole 2113, and a magnetic bead 12 is disposed in the elution tank 111b.

[0049] like Figure 1 , Figure 2 and Figure 5 As shown, sample tank 111a is used to hold samples and lysis buffer, washing tank 111c is used to hold washing buffer, elution tank 111b is used to hold elution buffer, and sample loading hole 2113 is rotated to communicate with sample tank 111a so that sample solution can be loaded into sample tank 111a through sample loading hole 2113. The sealing cap 25 can be placed inside sample loading hole 2113 to seal sample loading hole 2113, which can prevent external impurities from entering the shell body 211. In addition, the box body 112 also has multiple spaced liquid storage tanks 1123, which are used to hold processing reagents such as protease and mineral oil. The sample detection box structure has the advantages of high integration and good sealing performance.

[0050] During sample testing, the sealing cap 25 is first opened to allow the sample solution to be added into the sample tank 111a through the sample application port 2113. After the sample solution is added, the sealing cap 25 is installed in the sample application port 2113 to seal the port and prevent external impurities from entering the shell body 211. Next, the sample testing box is placed into the sample testing equipment. The pipette pump of the sample testing equipment picks up the pipette tip 23 and lifts it up to detach from the placement tank 1121. Then, by rotating the shell body 211, the transfer sleeve 212 is rotated to the position corresponding to the elution tank 111b. The magnetic rod 200 extends into the transfer sleeve 212 to adsorb the magnetic beads 12 in the elution tank 111b. The separator cap 22 separates the magnetic rod 200 and the magnetic bead 12 to prevent external impurities from entering the shell body 211. Then, the shell body 211 is rotated so that the transfer sleeve 212 rotates to the corresponding position in the sample slot 111a. The magnetic rod 200 rises to release the magnetic bead 12, allowing it to fall into the sample slot 111a. The mixing sleeve 300 reciprocates within the transfer sleeve 212 to push the elastic pushing part 222 of the separator cap 22, causing the elastic pushing part 222 to strike the liquid in the sample slot 111a to mix the sample solution, lysis buffer, and magnetic bead 12. Once the sample solution is fully lysed and the nucleic acids in the sample solution are adsorbed onto the magnetic bead 12, the magnetic rod 200 extends into the transfer sleeve 211a. Magnetic beads 12 are adsorbed inside the sleeve 212. Then, the shell body 211 drives the transfer sleeve 212 to rotate to the position corresponding to the washing tank 111c. The magnetic rod 200 rises, causing the magnetic beads 12 to fall into the washing tank 111c. The mixing sleeve rises and falls to push the elastic pushing part 222 to mix the magnetic beads 12 and the washing liquid, achieving thorough cleaning of nucleic acids. After the nucleic acids on the magnetic beads 12 have been washed, the magnetic rod 200 extends into the transfer sleeve 212 and adsorbs the magnetic beads 12. Then, the shell body 211 drives the transfer sleeve 212 to rotate to the position corresponding to the elution tank 111b. The magnetic rod 200 rises, causing the magnetic beads 12 to fall into the elution tank 111b, so that the nucleic acids adsorbed on the magnetic beads 12 are eluted in the elution liquid. To complete the purification of nucleic acid; after the nucleic acid purification is completed, the magnetic rod 200 extends into the transfer sleeve 212 and adsorbs the magnetic beads 12 in the elution tank 111b, so as to transfer the magnetic beads 12 to the sample tank 111a or the washing tank 111c. Then, the shell body 211 drives the pipette tip 23 to rotate to the position of the corresponding elution tank 111b, so that the pipette tip 23 can draw up the processed liquid in the elution tank 111b. Then, the shell body 211 drives the pipette tip 23 to rotate to the position of the corresponding injection tank 1134, so that the pipette tip 23 can inject the processed liquid into the injection tank 1134 and flow into the storage chamber 1132 along the guide slope 1136 to mix with the detection reagent or the lyophilized bulb for detection.After liquid injection is completed, the shell body 211 drives the push plunger 24 to rotate to the position corresponding to the liquid storage chamber 1132. The sample detection device pushes the push plunger 24 down along the guide hole 2112 and pushes the sealing plug 13 downward, so that the sealing plug 13 seals the liquid storage chamber 1132. This facilitates the PCR detection mechanism to detect the liquid in the liquid storage chamber 1132, eliminating the waste liquid removal step, effectively preventing waste liquid residue, and significantly improving the accuracy of sample detection results.

[0051] In this embodiment of the utility model, the shell body 211 includes a top plate 2114, a surrounding plate 2115 and a rotating column 2116. The surrounding plate 2115 surrounds the periphery of the top plate 2114, the transfer sleeve 212 passes through the top plate 2114, one end of the rotating column 2116 is connected to the top plate 2114, and the other end of the rotating column 2116 is rotatably passed through the liquid storage box 11.

[0052] like Figures 2 to 4 As shown, the upper end of the rotating column 2116 is connected to the top plate 2114, and the lower end of the rotating column 2116 passes through the liquid storage box 11 and is rotatably connected to the liquid storage box 11, so that the top plate 2114 and the surrounding plate 2115 can rotate relative to the liquid storage box 11. This allows the top plate 2114 to drive the transfer sleeve 212 to rotate to correspond with any of the pretreatment tanks 111. The surrounding plate 2115 plays a sealing and protective role, effectively preventing external impurities from entering the shell body 211, and further improving the detection accuracy. In addition, the liquid storage box 11 is provided with a sealing film, which is used to seal the washing tank 111c and the elution tank 111b to prevent impurities and contaminants from contaminating the washing liquid or elution liquid, further improving the sealing performance of the sample detection box.

[0053] In addition, the transfer sleeve 212 extends from the top plate 2114 toward the liquid storage box 11, increasing the distance between the top plate 2114 and the top of the liquid storage box 11. This allows the pipette tip 23 to pass through the pipette hole 2111 and extend into the placement slot 1121. The sample detection box integrates the pipette tip 23, enabling the sample detection equipment to directly pick up the pipette tip 23 for liquid transfer, effectively preventing the introduction of impurities and improving the structural integration.

[0054] In addition, this utility model also provides a sample detection device, which includes a sample detection box according to the above description. The specific structure of the sample detection box is as described in the above embodiments. Since the sample detection device adopts all the technical solutions of the above embodiments, it has at least all the beneficial effects brought about by the technical solutions of the above embodiments, which will not be described in detail here.

[0055] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0056] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0057] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0058] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A sample detection kit, characterized in that, The sample detection kit includes: The liquid storage assembly (10) includes a liquid storage box (11) and a magnetic bead (12). The liquid storage box (11) has multiple pretreatment slots (111), and the magnetic bead (12) is disposed in one of the pretreatment slots (111). The transfer assembly (20) includes a housing (21) and a separator cap (22). The housing (21) includes a shell body (211) and a transfer sleeve (212). The shell body (211) is rotatably mounted on the liquid storage box (11). The transfer sleeve (212) passes through the shell body (211) and can be rotated to be positioned in any of the pretreatment tanks (111). The first end of the transfer sleeve (212) is used for the magnetic rod (200) of the sample detection device to extend into and attract the magnetic beads (12). The separator cap (22) is located at the second end of the transfer sleeve (212) and seals the transfer sleeve (212). The separator cap (22) is used to separate the magnetic rod (200) and the magnetic beads (12).

2. The sample detection kit according to claim 1, characterized in that, The separator cap (22) includes a sleeve portion (221) and an elastic pushing portion (222). The sleeve portion (221) is sleeved on the second end of the transfer sleeve (212). The elastic pushing portion (222) is located at one end of the sleeve portion (221) and blocks the transfer sleeve (212). The first end of the transfer sleeve (212) is also used for the mixing sleeve (300) of the sample detection device to extend into and push the elastic pushing portion (222).

3. The sample detection kit according to claim 1, characterized in that, The liquid storage box (11) includes a box body (112) and a detection tube (113). The box body (112) has multiple pretreatment slots (111). The detection tube (113) passes through the box body (112) and includes a tube body (1131) and an injection section (1133). The tube body (1131) has a liquid storage chamber (1132), and the injection section (1133) is provided with… A liquid injection groove (1134) is provided on one side of the tube body (1131) and inside the liquid injection section (1133). A communication port (1135) communicating with the liquid storage chamber (1132) is provided on the side wall of the liquid injection groove (1134). The liquid storage assembly (10) also includes a sealing plug (13). The sealing plug (13) is movably disposed in the liquid storage chamber (1132) and is used to block the communication port (1135).

4. The sample detection kit according to claim 3, characterized in that, The bottom wall of the injection tank (1134) is provided with a flow guiding slope (1136), which is inclined from top to bottom in the direction toward the communication port (1135).

5. The sample detection kit according to claim 3, characterized in that, The transfer assembly (20) further includes a push plunger (24), and the shell body (211) is also provided with a guide hole (2112). The guide hole (2112) can be rotated to communicate with the liquid storage chamber (1132). The push plunger (24) is movably disposed in the guide hole (2112) and is used to push the sealing plug (13) to block the communication port (1135).

6. The sample detection kit according to claim 3, characterized in that, The box body (112) is also provided with a placement groove (1121). The detection tube (113), the placement groove (1121) and the multiple pretreatment grooves (111) are arranged circumferentially at intervals. The shell body (211) is provided with a pipetting hole (2111). The pipetting hole (2111) can be rotated to communicate with the injection groove (1134), the placement groove (1121) or any one of the pretreatment grooves (111). The transfer assembly (20) also includes a pipetting tip (23). The pipetting tip (23) passes through the pipetting hole (2111) and extends into the placement groove (1121).

7. The sample detection kit according to claim 3, characterized in that, The box body (112) is provided with a detection port (1122), and the number of detection tubes (113) is set to multiple, with the multiple detection tubes (113) arranged at intervals and each corresponding to the detection port (1122).

8. The sample detection kit according to any one of claims 1 to 7, characterized in that, The shell body (211) is provided with a sample feeding hole (2113). The plurality of pretreatment tanks (111) include a sample tank (111a), an elution tank (111b) and at least one washing tank (111c). The sample feeding hole (2113) can be rotated to communicate with the sample tank (111a). The transfer assembly (20) also includes a sealing cap (25) covering the sample feeding hole (2113).

9. The sample detection kit according to any one of claims 1 to 7, characterized in that, The shell body (211) includes a top plate (2114), a surrounding plate (2115), and a rotating column (2116). The surrounding plate (2115) surrounds the periphery of the top plate (2114). The transfer sleeve (212) passes through the top plate (2114). One end of the rotating column (2116) is connected to the top plate (2114), and the other end of the rotating column (2116) is rotatably disposed through the liquid storage box (11). And / or, the second end of the transfer sleeve (212) is provided with an installation step (2121), and the separator cap (22) is fitted onto the installation step (2121).

10. A sample testing device, characterized in that, The sample testing device includes a sample testing box according to any one of claims 1 to 9.

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