Culture apparatus for human mesenchymal stem cell culture isolation
By designing individual and overall control components, the problem of affecting other cells when observing culture plates was solved, achieving stable pressing and flexible control of culture dishes, reducing external vibrations, protecting equipment cleanliness, and improving operational convenience and equipment durability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- BEIJING ZHONGKE JIANLAN GRP CO LTD
- Filing Date
- 2025-06-10
- Publication Date
- 2026-06-26
AI Technical Summary
In existing technologies, the entire cell culture plate needs to be removed when observing it, which affects the development of other cells, and external vibrations and shocks affect the culture process.
Individual control components and overall control components were designed, including pressure plates, damping springs, push plates, and cover plates, to achieve stable pressing and flexible control of the petri dishes, reduce the impact of external vibrations, and prevent dust from entering through the cover plates.
It achieves stable pressing and flexible control of petri dishes, reduces the impact of external vibrations, protects equipment cleanliness, improves operational convenience and equipment durability, avoids waste, and ensures the smooth progress of scientific research.
Smart Images
Figure CN224411783U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, specifically a culture instrument for the culture and separation of human mesenchymal stem cells. Background Technology
[0002] Human mesenchymal stem cells (MSCs) are a type of cell with multi-directional differentiation potential, and they have shown broad application prospects in regenerative medicine, tissue engineering, and cell therapy. In order to fully realize their potential, increasingly higher requirements are being placed on the efficient culture and isolation technology of MSCs. Therefore, it is particularly important to develop a culture instrument specifically for the culture and isolation of human mesenchymal stem cells.
[0003] Publication (Announcement) No.: CN214270864U discloses a separate cell culture dish. A base is nested within a substrate, with the edge of the base sealingly contacting the inner wall of the substrate. A partition plate is fixed to the base, dividing it into multiple culture zones. An inner cover is flat and nested and sealed within the partition plate, making sealing contact with the top of the substrate. Feed holes are provided in the inner cover at positions relative to each culture zone. An outer cover is nested above the substrate, making sealing contact with the outer wall of the substrate. This invention can be combined to create different culture spaces to meet various cell culture needs.
[0004] However, during cell culture, if staff want to observe the culture in the grooves of a particular culture plate up close, they have to remove the entire cell culture plate from the culture room, which can easily affect the development of other cells. Utility Model Content
[0005] To address the shortcomings of existing technologies, this invention provides a culture device for the culture and separation of human mesenchymal stem cells, thus solving the technical problems mentioned in the background section.
[0006] To achieve the above objectives, this utility model is implemented through the following technical solution: a culture device for culturing and separating human mesenchymal stem cells, including a culture box, a frame fixedly connected to the top rear end of the culture box, a culture dish provided on the surface of the culture box, and a single control component and a total control component for fixing the position of the culture dish provided on the inner side of the frame;
[0007] The single control component includes pressure plates that are rotatably mounted on the left and right sides of the surface of the culture box. The front ends of the two pressure plates are used to press the culture dish. A third damping spring is fixedly connected between the rear ends of the two pressure plates. A push plate is rotatably connected to the top of the pressure plates. A first control plate is rotatably connected to the other end of the push plate. An auxiliary plate is fixedly connected to the inner end of the first control plate.
[0008] As a further preferred embodiment of this technical solution, a cover plate is rotatably connected to the front end of the frame, and a transparent glass plate is provided on the cover plate.
[0009] As a further preferred embodiment of this technical solution, a first engaging block is provided on the inner end of the frame, a sliding rod is slidably connected to one side of the first engaging block, and the sliding rod is fixedly connected to the inner wall of the frame, and a second damping spring is sleeved on the surface of the sliding rod. A pull plate is fixedly connected to the bottom of the first engaging block, and the pull plate is slidably installed on the frame.
[0010] As a further preferred embodiment of this technical solution, the main control assembly includes a second control plate located above the auxiliary plate, with guide rods slidably connected to both ends of the second control plate, and a fourth damping spring sleeved on the outer wall of the guide rods.
[0011] As a further preferred embodiment of this technical solution, a second mating block is fixedly connected to the outer end of the second control board, and the specifications of the second mating block are compatible with the specifications of the first mating block.
[0012] As a further preferred embodiment of this technical solution, the surface of the culture box is provided with a placement groove, the size of which is compatible with the size of the culture dish. A telescopic rod is provided at the bottom of the placement groove, a top platform is provided at the top of the telescopic rod, and a first damping spring is sleeved on the telescopic rod.
[0013] Compared with existing technologies, it has the following advantages:
[0014] By incorporating a single-function control component, stable pressure and flexible control of the culture dish are achieved. Furthermore, the inclusion of components such as telescopic rods and damping springs effectively reduces the impact of external vibrations and shocks on the culture process. These advantages make the single-function control component an indispensable part of cell culture experiments, providing strong support for the smooth progress of scientific research.
[0015] The cover design not only effectively blocks external dust and protects the cleanliness of the equipment's interior, but also enables the flexible movement and reset of the first mating block through the cooperation of the pull plate and the second damping spring. This design not only improves the practicality and durability of the equipment, but also brings users a more convenient and efficient user experience.
[0016] By setting up individual control components and a central control component, staff can freely select the quantity and assemble cells during cell culture, avoiding waste and saving resources. Attached Figure Description
[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0018] Figure 2 This is a schematic diagram of the structure of the individual control component and the overall control component in this utility model;
[0019] Figure 3 This is a schematic diagram of the structure of a single-item control component in this utility model;
[0020] Figure 4 for Figure 2 Enlarged view of point A in the middle;
[0021] Figure 5 This is a schematic diagram of the structure of the culture box and culture dish in this utility model.
[0022] In the diagram: 1. Culture box; 2. Frame; 3. Petri dish; 4. Individual control component; 5. Main control component; 11. Telescopic rod; 12. Top platform; 13. First damping spring; 21. Cover plate; 22. First mating block; 23. Pull plate; 24. Slide rod; 25. Second damping spring; 41. Pressure plate; 42. Third damping spring; 43. Push plate; 44. First control plate; 45. Auxiliary plate; 51. Second control plate; 52. Guide rod; 53. Fourth damping spring; 54. Second mating block. Detailed Implementation
[0023] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0024] Example 1: Combining Figures 1-5 As shown, this utility model provides a technical solution: a culture device for culturing and separating human mesenchymal stem cells, including a culture box 1, a frame 2 fixedly connected to the top rear end of the culture box 1, a culture dish 3 disposed on the surface of the culture box 1, and a single control component 4 and a total control component 5 for fixing the position of the culture dish 3 disposed on the inner side of the frame 2.
[0025] The single-item control component 4 is an indispensable part of the cell culture equipment. It mainly consists of the left and right pressure plates 41, the third damping spring 42, the push plate 43, the first control plate 44, and the auxiliary plate 45. These components, through careful design and ingenious assembly, together constitute this powerful control unit.
[0026] Two pressure plates 41 are rotatably mounted on the surface of the culture box 1, located on its left and right sides respectively. Their front ends are used to press down the culture dish 3 to ensure the stability of the culture dish 3 during the culture process. At the same time, a third damping spring 42 is fixedly connected between the rear ends of the pressure plates 41. This design enables the pressure plates 41 to have a certain buffering and restoring ability when subjected to external force, thereby protecting the culture dish 3 and the culture from the impact and vibration.
[0027] Next is the push plate 43, which is rotatably connected to the top of the pressure plate 41. The other end of the push plate 43 is rotatably connected to the first control plate 44. This connection method allows the push plate 43 to rotate freely within a certain range, thereby realizing flexible control of the first control plate 44. An auxiliary plate 45 is fixedly connected to the inner end of the first control plate 44. This structure enhances the stability and load-bearing capacity of the first control plate 44, enabling it to better undertake the control task.
[0028] A placement groove is provided on the surface of the culture box 1. The size of the placement groove is compatible with the size of the culture dish 3. This design allows the frame 2 to be easily placed in the placement groove, thereby achieving stable support for the culture dish 3. A telescopic rod 11 is provided at the bottom of the placement groove, and a top platform 12 is provided at the top of the telescopic rod 11. This structure allows the top platform 12 to move up and down under the action of the telescopic rod 11. A first damping spring 13 is also sleeved on the telescopic rod 11. This design gives the top platform a certain damping effect during movement, reducing vibration and displacement caused by external forces, and ensuring the stability and safety of the culture dish.
[0029] In an embodiment of the present invention, the front end of the pressure plate 41 can rotate inward under the elastic force of the third damping spring 42. This design enables the pressure plate 41 to press and position the top of the culture dish 3, ensuring that the culture dish remains stable in a specific position. In addition, the first control plate 44 and the push plate 43 also play important roles. When it is necessary to remove the culture dish, the operator can move the first control plate 44 upward. This action will work with the push plate 43 to drive the rear ends of the two pressure plates 41 to rotate inward.
[0030] During this process, the third damping spring 42 is compressed, so that the front end of the pressure plate 41 no longer presses against the culture dish 3. In this way, the operator can easily remove the culture dish 3. It is worth noting that the elastic force of the damping spring 42 plays a key role in this process. It can not only ensure that the pressure plate 41 can tightly press the culture dish 3 when not needed, but also smoothly release the pressure when needed, making the removal of the culture dish 3 easy and free.
[0031] To better understand this process, we can analyze it in depth from the following aspects:
[0032] First, the elastic force of the third damping spring 42 is crucial for the positioning of the pressure plate 41. Under normal use, the front end of the pressure plate 41 presses tightly against the top of the culture dish 3 under the action of the third damping spring 42. This design not only ensures the stability and safety of the culture dish 2, but also avoids displacement or damage to the culture dish 3 due to accidental collisions or vibrations. Second, when it is necessary to remove the culture dish 3, the upward movement of the first control plate 44 is the key to the entire operation. This action drives the rear end of the pressure plate 41 to rotate inward by pushing the plate 43. During this process, the third damping spring 42 is gradually compressed, so that the front end of the pressure plate 41 gradually releases its pressure on the culture dish 3. This design cleverly utilizes mechanical principles to achieve easy removal of the culture dish 3.
[0033] In summary, the single-item control component 4 plays a crucial role in cell culture equipment. Through precise design and ingenious assembly, it achieves stable pressure and flexible control over the culture dish 3. At the same time, through the setting of components such as telescopic rods and damping springs 42, it effectively reduces the impact of external vibrations and shocks on the culture process. These advantages make the single-item control component an indispensable part of cell culture experiments, providing a strong guarantee for the smooth progress of scientific research.
[0034] Place the culture dish 3 into the placement slot. The culture dish 3 is located on top of the top platform 12. When the pressure plate 41 presses the culture dish 3 downward, the top platform 12 compresses the first damping spring 13, causing the telescopic rod 11 to retract. When the pressure plate 41 no longer presses the culture dish 3, under the elastic force of the first damping spring 13, the top platform 12 moves the culture dish 3 upward, thus facilitating the removal of the culture dish 3.
[0035] Example 2: Combination Figure 1 , Figure 2 , Figure 4 As shown, based on Embodiment 1, the front end of the frame 2 is rotatably connected to the cover plate 21. This connection method allows the cover plate 21 to rotate at the front end of the frame 2, thereby realizing the function of opening or closing. A first engaging block 22 is provided on the inner end of the frame 2. One side of the slide rod 24 is slidably connected to the first engaging block 22, while the other side is fixedly connected to the inner wall of the frame 2. This connection method allows the slide rod 24 to slide along the first engaging block 22 under its drive, while also maintaining a stable connection between the slide rod 24 and the frame 2. This movement mode of the slide rod 24 makes the entire mechanical structure more flexible during operation and also enhances the stability of the structure.
[0036] In addition, a second damping spring 25 is also fitted on the surface of the slide bar 24. The second damping spring 25 is a spring with damping function, which is usually used to absorb or reduce the impact and vibration in mechanical movement. Here, the second damping spring 25 is set in order to reduce the impact and vibration generated by the slide bar 24 during sliding, thereby protecting the entire mechanical structure from damage.
[0037] Finally, a pull plate 23 is fixedly connected to the bottom of the first mating block 22, and the pull plate 23 is slidably mounted on the frame 2. This means that the pull plate 23 can slide on the frame 2, while its position is fixedly restricted by the first mating block 22. The first mating block 22 can be driven to move by pulling the pull plate 23.
[0038] In the embodiments of the present invention, the cover plate 21 is a key component of the equipment, and its importance is self-evident. Its main function is to block external dust and ensure the cleanliness and safety of the equipment. In order to better observe the operation of the equipment, a transparent glass is specially provided on the cover plate 21, so that users can clearly see the operating status of the equipment without opening the cover plate.
[0039] Users can pull the pull plate 23 to make the first engaging block 22 slide on the slide bar 24. When the first engaging block 22 moves outward, it will compress the second damping spring 25. This is to ensure the smoothness and stability of the sliding process. Once the second engaging block 54 and the second control plate 51 have completed their downward movement, the user can release the pull plate 23. At this time, the second damping spring 25 will push the first engaging block 22 back to its original position under its elastic force. This design not only improves the ease of operation of the equipment, but also enhances the service life and stability of the equipment.
[0040] In summary, the ingenious design of the cover plate 21 and its related components not only effectively blocks external dust and protects the cleanliness of the equipment's interior, but also enables the flexible movement and reset of the first mating block 22 through the cooperation of the pull plate 23 and the second damping spring 25. This design not only improves the practicality and durability of the equipment, but also brings users a more convenient and efficient user experience.
[0041] Example 3: Combination Figure 4As shown, based on Embodiment 2, the main control assembly 5 is mainly composed of components such as the second control plate 51, guide rod 52, fourth damping spring 53, and second mating block 54. The second control plate 51, as the core component of the main control assembly 5, bears the important task of control and adjustment. In order to ensure that it can move smoothly and accurately, the designers cleverly adopted the guide rod 52. These two sturdy rods are fixed on the top of the culture box 1, providing a stable sliding track for the second control plate 51. When the second control plate 51 slides on the guide rod 52, it can ensure that the parameter changes of the culture environment are uniform and continuous, thereby meeting the various needs of cell growth.
[0042] To ensure the stability and smoothness of the second control plate 51 during the sliding process, the designers also fitted a fourth damping spring 53 on the outer wall of the guide rod 52. One end of this spring is fixedly installed on the top of the culture box 1, and the other end is tightly connected to the bottom of the second control plate 51. When the second control plate 51 slides on the guide rod 52, the fourth damping spring 53 can provide appropriate resistance, making the movement of the second control plate 51 more stable and avoiding adverse effects on cell growth due to movement that is too fast or too slow.
[0043] In addition, to enhance the flexibility and adjustability of the main control assembly 5, the designers also fixedly connected a second mating block 54 to the outer end of the second control board 51. The design of this mating block is very ingenious, and its specifications are perfectly matched with the first mating block 22.
[0044] In this embodiment of the invention, the design of the second control plate 51 is the core part of the entire petri dish removal process. The second control plate 51 is slidably mounted on the frame 2 in the vertical direction. Through a precise mechanical structure, it enables convenient removal of the petri dishes 3. When the experimenter needs to remove all the petri dishes, they only need to control the second control plate 51 to move upward. As the second control plate 51 rises, a series of ingenious linkage mechanisms begin to work. The second control plate 51 works closely with the auxiliary plate 45, driving all the first control plates 44 to move upward together. During this process, the first control plates 44 interact with the push plate 43, further driving the pressure plate 41 to rotate outward from the petri dish 3. This action not only compresses the third damping spring 42, but also causes the pressure plate 41 to rotate outward from the petri dish 3. The top of the culture dish 3 is moved away, creating space for removing the culture dish. At the same time, the rise of the second control plate 51 also drives the rise of the second mating block 54, stretching the fourth damping spring 53. As the second mating block 54 rises, it gradually pushes the first mating block 22 to slide outward. During this sliding process, the first mating block 22 compresses the second damping spring 25 until the second mating block 54 moves to the top of the first mating block 22. At this time, the elastic force of the second damping spring 25 causes the first mating block 22 to move to the bottom of the second mating block 54 and forms a support for the second mating block 54. This support structure effectively prevents the second control plate 51 from moving downward under the elastic force of the fourth damping spring 53, thereby ensuring that all pressure plates 41 are in the unfolded state.
[0045] After this series of mechanical actions are completed, the experimenter can easily remove all the petri dishes 3 without having to operate them one by one, which greatly improves work efficiency. In addition, this design also ensures the safety of the petri dish removal process and avoids the risk of petri dish damage or experimental data loss due to improper operation.
[0046] The working principle of the culture device used for the culture and isolation of human mesenchymal stem cells is as follows: human mesenchymal stem cells are placed in culture dish 3, and then culture dish 3 is placed in the placement slot. Culture dish 3 is located on the top of the top platform 12. Under the elastic force of the third damping spring 42, the front end of the pressure plate 41 can be rotated inward and pressed and positioned on the top of culture dish 3.
[0047] When it is necessary to remove a single culture dish 3, move the first control plate 44 upward, and in conjunction with the push plate 43, drive the rear ends of the two pressure plates 41 to rotate inward and compress the third damping spring 42, so that the front end of the pressure plate 41 no longer presses against the culture dish 3, and then remove the culture dish 3.
[0048] When all the culture dishes 3 need to be removed, the second control plate 51 moves upward. The second control plate 51, in conjunction with the auxiliary plate 45, moves all the first control plates 44 upward. The first control plates 44, in conjunction with the push plate 43, rotate the pressure plate 41 outward from the culture dish 3, compressing the third damping spring 42. Simultaneously, as the second control plate 51 moves upward, it moves the second mating block 54 upward, stretching the fourth damping spring 53. During the upward movement of the second mating block 54, it pushes the first mating block 22 outward, compressing the second damping spring 25. When the second mating block 54 reaches the top of the first mating block 22, the first mating block 22, under the elastic force of the second damping spring 25, pushes itself to the bottom of the second mating block 54, providing support and preventing the second control plate 51 from moving downward under the elastic force of the fourth damping spring 53. At this point, all the pressure plates 41 are in the unfolded state, and then all the culture dishes 3 can be removed.
[0049] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A culture apparatus for culturing and isolating human mesenchymal stem cells, comprising a culture box (1), characterized in that: The rear top of the culture box (1) is fixedly connected to a frame (2), and a culture dish (3) is provided on the surface of the culture box (1). The inner end of the frame (2) is provided with a single control component (4) and a total control component (5) for fixing the position of the culture dish (3). The single control component (4) includes pressure plates (41) that are rotatably mounted on the left and right sides of the surface of the culture box (1). The front ends of the two pressure plates (41) are used to press the culture dish (3). A third damping spring (42) is fixedly connected between the rear ends of the two pressure plates (41). A push plate (43) is rotatably connected to the top of the pressure plate (41). A first control plate (44) is rotatably connected to the other end of the push plate (43). An auxiliary plate (45) is fixedly connected to the inner end of the first control plate (44).
2. The culture apparatus for culturing and isolating human mesenchymal stem cells according to claim 1, wherein: The front end of the frame (2) is rotatably connected to a cover plate (21), and a transparent glass is provided on the cover plate (21).
3. The culture apparatus for culturing and isolating human mesenchymal stem cells according to claim 1, wherein: A first fitting block (22) is provided on the inner end of the frame (2). A slide rod (24) is slidably connected to one side of the first fitting block (22), and the slide rod (24) is fixedly connected to the inner wall of the frame (2). A second damping spring (25) is sleeved on the surface of the slide rod (24). A pull plate (23) is fixedly connected to the bottom of the first fitting block (22), and the pull plate (23) is slidably installed on the frame (2).
4. The culture apparatus for culturing and isolating human mesenchymal stem cells according to claim 3, wherein: The main control assembly (5) includes a second control plate (51) located above the auxiliary plate (45). Guide rods (52) are slidably connected to both ends of the second control plate (51), and a fourth damping spring (53) is sleeved on the outer wall of the guide rods (52).
5. The culture apparatus for culturing and isolating human mesenchymal stem cells according to claim 4, wherein: The outer end of the second control plate (51) is fixedly connected to a second mating block (54), and the specifications of the second mating block (54) are compatible with the specifications of the first mating block (22).
6. The culture apparatus for culturing and isolating human mesenchymal stem cells according to claim 1, wherein: The surface of the culture box (1) is provided with a placement groove, the size of which is compatible with the size of the culture dish (3). A telescopic rod (11) is provided at the bottom of the placement groove, a top platform (12) is provided at the top of the telescopic rod (11), and a first damping spring (13) is sleeved on the telescopic rod (11).