A plant tissue culture light environment experimental device

Abstract

This utility model belongs to the field of plant cultivation technology and provides a plant tissue culture light environment experimental device, including a box, a cover plate, LED supplemental lights, a fixing rod, a heating device, and a support plate. The top of the box has an opening, within which a suitable cover plate is placed. The cover plate is fixedly connected to the support plate via the fixing rod. The support plate is located below the cover plate and inside the box, and is used to place multiple culture bottles containing culture medium and tissues to be cultured. A heating medium is provided inside the box, and the heating device is located at the bottom of the box for heating the heating medium. The bottoms of the culture bottles and the support plate are both located within the heating medium. LED supplemental lights are installed on the inner walls of the box. This utility model achieves coordinated control of light and temperature, providing a stable and controllable experimental environment for plant tissue culture.

Description

Technical Field

[0001] This utility model belongs to the field of plant cultivation technology, specifically relating to a plant tissue culture light environment experimental device. Background Technology

[0002] Virus-free cultivation refers to the process of avoiding contamination of plant cultures by harmful microorganisms and chemicals during plant tissue culture, ensuring the purity and health of the cultures, thereby improving the success rate and quality of plant cultivation. According to long-term scientific research, the main reason for the decline in crop quality is infection by plant viruses. To achieve high yields, it is essential to cultivate virus-free seedlings, also known as virus-free seedlings, which is the concept of virus-free seedlings.

[0003] In existing technologies, the process of cultivating virus-free seedlings typically involves the use of culture bottles, grow lights, and heating devices. Heating devices generally fall into two categories: ambient air heating and lamp heating. Ambient air heating is similar to an air conditioner, but it consumes a lot of electricity; while lamp heating suffers from uneven heating. Furthermore, since the lamps are usually installed above the culture bottles, their heating effect on the roots and culture medium is significantly affected by the growth and shading of the plant foliage. Utility Model Content

[0004] To solve the above-mentioned technical problems, this utility model provides a plant tissue culture light environment experimental device to address the issues in the prior art. The technical solution adopted by this utility model is as follows:

[0005] An experimental device for light environment in plant tissue culture includes a box, a cover plate, an LED supplemental light, a fixing rod, a heating device, and a support plate;

[0006] The top of the box is provided with an opening, and a suitable cover plate is provided in the opening. The cover plate is fixedly connected to the support plate by the fixing rod.

[0007] The support plate is located below the cover plate and inside the box. The support plate is used to place multiple culture bottles, and the culture bottles contain culture medium and tissues to be cultured.

[0008] The chamber contains a heating medium, and the heating device is located at the bottom of the chamber for heating the heating medium; the bottom of the culture bottle and the support plate are both located within the heating medium.

[0009] The LED fill lights are installed on all four inner walls of the enclosure.

[0010] Furthermore, the top of the box is provided with a reduced diameter section, the hollow part of the reduced diameter section is connected to the interior of the box, the inner diameter of the reduced diameter section is smaller than the inner diameter of the box, the cover plate is disposed inside the reduced diameter section, and the size of the support plate is adapted to the interior of the reduced diameter section.

[0011] Furthermore, the cover plate is made of a transparent material.

[0012] Furthermore, a top plate is provided on the top end face of the reduced diameter section. The top plate has a hollow structure. The top of the fixing rod passes through the cover plate and the top plate in sequence. An adjusting bolt is provided on the fixing rod, and the adjusting bolt abuts against the top of the top plate.

[0013] Furthermore, the inner walls of the enclosure are all equipped with heat-insulating and reflective panels.

[0014] Furthermore, the culture bottle is a transparent, sealed bottle or an open container.

[0015] Furthermore, when the culture bottle is a transparent sealed bottle, the heating medium is pure water, and the culture medium and the tissue to be cultured are located in the sealed space of the transparent sealed bottle.

[0016] Furthermore, when the culture flask is an open container, the heating medium is a mixed solution of water and culture medium, the tissue to be cultured is placed inside the open container, and the heating medium is connected to the interior of the open container.

[0017] Furthermore, the open container includes a cylindrical body and a base, with the bottom of the cylindrical body fitted onto the base, and a trough for placing the tissue to be cultured provided on the base. The cylindrical body is a cage-type cylindrical body or a grid-type cylindrical body.

[0018] The present invention has the following beneficial effects: The present invention achieves coordinated control of light and temperature, providing a stable and controllable experimental environment for plant tissue culture. Through direct contact between the heating medium and the culture bottle, efficient heat conduction can be achieved, reducing energy consumption. The heat is directly applied to the substrate, ensuring the uniformity and stability of the temperature of the culture medium and the tissue to be cultured. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of an embodiment of the transparent sealed bottle of this utility model;

[0020] Figure 2 This is a schematic diagram of an open container embodiment of the present invention;

[0021] Figure 3 yes Figure 2 Enlarged view of point A in the middle;

[0022] Figure 4This is a schematic diagram of a cage-type cylinder;

[0023] Figure 5 This is a diagram illustrating the process of placing and removing culture bottles. Detailed Implementation

[0024] The following will be based on the embodiments of this utility model. Figures 1-5 The technical solutions in the embodiments of this utility model are clearly and completely described. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0025] like Figure 1 A plant tissue culture light environment experimental device includes a box 2, a cover plate 7, an LED supplemental light 8, a fixing rod 9, a heating device 13, and a support plate 14;

[0026] The top of the box 2 is provided with an opening, and a suitable cover plate 7 is provided in the opening. The cover plate 7 is fixedly connected to the bearing plate 14 by the fixing rod 9.

[0027] The support plate 14 is located below the cover plate 7 and inside the box 2. The support plate 14 is used to place multiple culture bottles, and the culture bottles are filled with culture medium and tissues to be cultured.

[0028] The box 2 is equipped with a heating medium 1, and the heating device 13 is installed at the bottom of the box 2 for heating the heating medium 1; the bottom of the culture bottle and the support plate 14 are both located inside the heating medium 1.

[0029] The LED fill lights 8 are installed on all four inner walls of the housing 2.

[0030] Both the culture medium and the tissue to be cultured are existing technologies. The preferred tissue is the stem tip of a plant, as the auxin content in the stem tip meristem is very high, which can inhibit virus proliferation. Healthy plants free from diseases and pests and of pure varieties are selected. Stem tips of 2-3 cm with growth points and 1-2 leaf primordia are cut off, sterilized, and then placed in the culture medium for cultivation. The culture medium is prepared by mixing inorganic salts, trace elements, organic components, plant hormones, sugars, and agar. Generally, a high concentration of ammonium and potassium salts is beneficial to the survival of the stem tips.

[0031] In practice, the culture bottle is placed on the support plate 14, and the heating medium 1 is pre-filled into the box 2, immersing the bottom of the culture bottle and the support plate 14 in the medium. The cover plate 7 is then placed on top, and the support plate 14 is fixed in place by the fixing rod 9. The heating device 13 is activated to heat the heating medium 1, and the surrounding LED supplementary lights 8 are simultaneously turned on. By adjusting the heating temperature and the intensity of the supplementary lights, different light and temperature environments are simulated to conduct plant tissue culture experiments. The heating device 13 heats the heating medium 1, and the temperature inside the culture bottle is kept stable through heat conduction. The surrounding LED supplementary lights 8 provide uniform illumination to meet the photosynthetic needs of plant tissues. This invention achieves coordinated control of light and temperature, providing a stable and controllable experimental environment for plant tissue culture. Direct contact between the heating medium 1 and the culture bottle enables efficient heat conduction, reducing energy consumption. The heat acts directly on the substrate, ensuring the uniformity and stability of the temperature of the culture medium and the tissue to be cultured.

[0032] Furthermore, the support plate 14 is preferably a grid plate structure to increase the contact area between the heating medium 1 and the culture flask. The heating device is existing technology, such as a heating rod or an electric heating wire.

[0033] Furthermore, the top of the box body 2 is provided with a reduced diameter section 4, the hollow part of the reduced diameter section 4 is connected to the interior of the box body 2, the inner diameter of the reduced diameter section 4 is smaller than the inner diameter of the box body 2, the cover plate 7 is disposed inside the reduced diameter section 4, and the size of the support plate 14 is adapted to the interior of the reduced diameter section 4.

[0034] The reduced diameter section 4 forms a stepped structure, creating an inverted T-shaped structure together with the interior of the housing 2. The cover plate 7 and the support plate 14 are the same size and fit into the interior of the reduced diameter section 4, thus separating the inner wall of the housing 2 from the support plate 14 and the cover plate 7 by a certain space. The LED supplementary light 8 is installed within this space, avoiding interference with the vertical movement of the support plate 14. The cover plate 7 and the reduced diameter section 4 form a sealed structure, creating a closed space inside the housing 2 to prevent external contamination from entering.

[0035] Furthermore, the cover plate 7 is made of a transparent material; for example, plastic or glass.

[0036] Furthermore, a top plate 5 is provided on the top end face of the reduced diameter part 4. The top plate 5 is a hollow structure. The top of the fixing rod 9 passes through the cover plate 7 and the top plate 5 in sequence. An adjusting bolt 10 is provided on the fixing rod 9, and the adjusting bolt 10 abuts against the top of the top plate 5.

[0037] The top plate 5 has a relatively large hollow structure, which is intended to allow observation of the interior of the chamber 2 through the transparent cover 7, and to directly observe the growth status of the tissues in the culture bottle. The top plate 5 abuts against the top end face of the narrowed section 4, thereby providing support and suspending the support plate 14 in the air. The height of the support plate 14 can be adjusted by adjusting the height of the adjusting bolt 10, thus adjusting the water depth position of the support plate 14.

[0038] In addition, multiple fixing rods 9 can be set, and a frame 12 is fixedly connected to the fixing rod 9. The frame 12 is provided with multiple through holes, and the multiple through holes are fitted one-to-one on the culture bottle to constrain the position of the culture bottle.

[0039] like Figure 1 , Figure 5 When placing or removing the support plate 14 and the culture bottle, the top plate 5 can be lifted by the handle 6 at the top of the top plate 5, so that the support plate 14 and the cover plate 7 can pass through the narrowed section 4 to remove the culture bottle.

[0040] Furthermore, the inner walls of the enclosure 2 are all equipped with heat-insulating reflective panels 3. The heat-insulating reflective panels 3 reflect the light from the LED supplementary lights 8, while reducing heat exchange between the inside and outside of the enclosure 2.

[0041] The culture bottle of this utility model is a transparent sealed bottle 11 or an open container 15, including the following two embodiments:

[0042] Implementation method 1: such as Figure 1 When the culture bottle uses a transparent sealed bottle body 11, the heating medium 1 is pure water, and the culture medium and the tissue to be cultured are in the sealed space of the transparent sealed bottle body 11.

[0043] The transparent sealed bottle 11 is a conventional bottle in the prior art. In this embodiment, different plant tissues can be placed in each culture bottle, which is convenient for simultaneous cultivation of various tissues and is suitable for control experiments of virus-free seedling cultivation.

[0044] Implementation method 2: such as Figures 2-4 When the culture bottle is an open container 15, the heating medium 1 is a mixed solution of water and culture medium, the tissue to be cultured is placed inside the open container 15, and the heating medium 1 is connected to the interior of the open container 15.

[0045] A mixed solution of water and culture medium is pre-injected into the chamber 2 as heating medium 1. The tissue to be cultured is placed in an open container 15, allowing the interior of the container to communicate with the mixed solution. The mixed solution maintains the temperature through heating while simultaneously providing nutrients to the tissue. The mixed solution serves as both a heat transfer medium and a nutrient source, directly contacting the tissue through the open container 15, thus achieving simultaneous temperature control and nutrient supply. Implementation method 2 is suitable for culturing plant tissues from the same plant species, which require the same nutrient source. Therefore, it adopts a centralized nutrient supply method, simplifying experimental operations and eliminating the need for separate nutrient supply to the tissues. This method is suitable for studying the growth status of tissues in a dynamic nutrient environment.

[0046] Furthermore, the open container 15 includes a cylindrical body 151 and a base 153. The bottom of the cylindrical body 151 is fitted onto the base 153. The base 153 is provided with a trough 152 for placing the tissue to be cultured. The cylindrical body 151 is a cage-type cylindrical body or a grid-type cylindrical body.

[0047] The tissue to be cultured is placed in the trough 152 of the base 153, and then fitted with a cage-like or grid-like cylinder 151, which is placed on the support plate 14. The heating medium 1 enters the container through the pores of the cylinder 151 and comes into contact with the tissue. The cage-like or grid-like cylinder allows the heating medium 1 to flow freely, ensuring full contact between the tissue and the heating medium, while fixing the tissue position, restraining plant expansion, and preventing the tissue from shifting with the flow of the solution. The trough 152 of the base 153 provides a stable growth carrier for the tissue.

[0048] like Figure 4 The diagram shows a cage-like structure, which consists of multiple rings and rods. The rods are fixedly connected to the multiple rings to form an open cage structure.

[0049] In addition, to meet the absorption requirements of multiple cultured plants arranged in an array for various light quality parameters, the LED supplemental light 8 of this invention uses an array of multiple low-power LED beads to achieve better light uniformity. The total power of each LED module in the system is set to 9W, with 18 LED beads arranged in a 9×2 linear configuration, and a total rated operating current of 300mA. The LED supplemental light 8 is connected to a controller, which uses an STM8S105K4 microcontroller. Temperature and humidity sensors and light intensity sensors are installed inside the housing 2. In specific experiments, the LED supplemental light 8 can emit different spectra to supplement the plants, exploring the effects of different spectra on plant tissue culture. Specific experimental methods can be found in the existing literature "Design of Intelligent LED Plant Tissue Culture System" (DOI: 10.16661 / j.cnki.1672-3791.2401-5042-0299).

[0050] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Any modifications, alterations, alterations, or substitutions made by those skilled in the art to the technical solutions of the present utility model without departing from the spirit of the present utility model shall fall within the protection scope defined by the claims of the present utility model.

Claims

1. A plant tissue culture light environment experimental device, characterized in that, Includes a housing (2), a cover plate (7), an LED fill light (8), a fixing rod (9), a heating device (13), and a support plate (14); The top of the box (2) is provided with an opening, and a suitable cover plate (7) is provided in the opening. The cover plate (7) is fixedly connected to the bearing plate (14) by the fixing rod (9). The support plate (14) is located below the cover plate (7) and inside the box (2). The support plate (14) is used to place multiple culture bottles, and the culture bottles contain culture medium and tissues to be cultured. The box (2) is provided with a heating medium (1) inside, and the heating device (13) is provided at the bottom of the box (2) for heating the heating medium (1); the bottom of the culture bottle and the support plate (14) are both located inside the heating medium (1); The LED fill lights (8) are provided on all four inner walls of the box (2).

2. The plant tissue culture light environment experimental device according to claim 1, characterized in that, The top of the box (2) is provided with a reduced diameter section (4), the hollow part of the reduced diameter section (4) is connected to the inside of the box (2), the inner diameter of the reduced diameter section (4) is smaller than the inner diameter of the box (2), the cover plate (7) is disposed inside the reduced diameter section (4), and the size of the bearing plate (14) is adapted to the inside of the reduced diameter section (4).

3. The plant tissue culture light environment experimental device according to claim 2, characterized in that, The cover plate (7) is made of a transparent material.

4. The plant tissue culture illumination environment experimental device according to claim 2, characterized in that, The top end face of the reduced diameter part (4) is provided with a top plate (5), the top plate (5) is a hollow structure, the top of the fixing rod (9) passes through the cover plate (7) and the top plate (5) in sequence, and the fixing rod (9) is provided with an adjusting bolt (10), the adjusting bolt (10) abuts against the top of the top plate (5).

5. The experimental apparatus for plant tissue culture light environment according to claim 1, characterized in that, The inner walls of the box (2) are all equipped with heat-insulating reflective plates (3).

6. A plant tissue culture light environment experimental device according to any one of claims 1-5, characterized in that, The culture bottle is a transparent, sealed bottle (11) or an open container (15).

7. The plant tissue culture light environment experimental device according to claim 6, characterized in that, When the culture bottle is a transparent sealed bottle (11), the heating medium (1) is pure water, and the culture medium and the tissue to be cultured are in the sealed space of the transparent sealed bottle (11).

8. The experimental apparatus for plant tissue culture light environment according to claim 6, characterized in that, When the culture bottle is an open container (15), the heating medium (1) is a mixed solution of water and culture medium, the tissue to be cultured is placed inside the open container (15), and the heating medium (1) is connected to the interior of the open container (15).

9. The experimental apparatus for plant tissue culture light environment according to claim 8, characterized in that, The open container (15) includes a cylindrical body (151) and a base (153). The bottom of the cylindrical body (151) is fitted onto the base (153). A trough (152) for placing the tissue to be cultured is provided on the base (153). The cylindrical body (151) is a cage-type cylindrical body or a grid-type cylindrical body.

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