A highly efficient device for isolating and identifying bacterial communities
By designing an efficient microbial community separation and identification device, and utilizing a sterile coating roller and guiding system, the problem of uneven manual coating was solved, achieving uniformity and stability of microbial community separation and improving the efficiency of microbial community separation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- JIANGSU DAO BIO-ENVIRONMENTAL TECH CO LTD
- Filing Date
- 2025-07-21
- Publication Date
- 2026-06-30
AI Technical Summary
In existing plating methods, uneven manual coating leads to poor bacterial isolation.
A separation and identification device comprising a base plate, a vertical plate, a support plate, and a coating structure was designed. A sterile coating roller and a guiding system are used to ensure coating uniformity, and the culture medium plate is positioned and stabilized by springs and abutment grooves.
This technology enables the aseptic coating roller to maintain high stability without being affected by hand force, ensuring coating uniformity. Furthermore, the combination of the spring sheet and the abutment groove ensures stable installation of the culture medium plate, thereby improving the efficiency of bacterial isolation.
Smart Images
Figure CN224430569U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of bacterial community isolation and identification technology, specifically to a highly efficient bacterial community isolation and identification device. Background Technology
[0002] Microbial isolation refers to the process of separating different microbial species from a mixed sample containing multiple microorganisms, and finally obtaining a single, pure microbial strain.
[0003] The steps involved in bacterial isolation include sample collection and processing, isolation and culture, and purification. Among these, isolation and culture are the key steps, and various isolation methods can be used. The plating method is one such method. In the plating method, a certain amount of sample dilution is taken and added to a solid culture medium plate, and then evenly spread on the surface of the medium using a sterile spreader. When the dilution is appropriate, single, dispersed colonies can also be formed.
[0004] The typical plating method uses a sterile plating stick for separation, which involves manual application. However, the application is not very uniform due to the force exerted by the hand.
[0005] In view of this, we propose a highly efficient device for the isolation and identification of bacterial communities. Utility Model Content
[0006] The purpose of this invention is to provide a highly efficient device for isolating and identifying bacterial communities, so as to solve the problems mentioned in the background art.
[0007] To achieve the above objectives, the present invention provides the following technical solution: a highly efficient microbial community isolation and identification device, comprising a base plate, wherein vertical plates are fixedly arranged on both sides of the upper surface of the base plate, and support plates are fixedly arranged on the bottom of the opposite side of the two vertical plates, the two support plates are used to support the culture medium plate, the two support plates are in contact with the lower surface of the culture medium plate, and tracks are fixedly arranged on the top of the opposite side of the two vertical plates, the two tracks are used to provide path guidance for the movement of the coating structure;
[0008] The coating structure includes a movable plate and a connecting plate. The two ends of the movable plate slide along two tracks respectively. A screw is threaded through the movable plate. A rotating handle is fixedly provided at the top of the screw. The connecting plate is rotatably connected to the bottom of the screw through a rotating shaft. A sterile coating roller is provided at the bottom of the connecting plate.
[0009] A guide rod is provided on the movable plate, and the bottom end of the guide rod is fixedly connected to the connecting plate.
[0010] Preferably, the bottom ends of the connecting plate are provided with buckles, and the shafts at both ends of the aseptic coating roller are respectively engaged with the two buckles.
[0011] Preferably, the shafts at both ends of the sterile coating roller are rotatable relative to the two latches.
[0012] Preferably, the track is closed at both ends.
[0013] Preferably, each of the two vertical plates is provided with a spring piece on its opposite side. The spring piece is arc-shaped, and the arched end of the spring piece abuts against the groove opened on the side of the culture medium plate.
[0014] Preferably, guide plates are fixedly provided at the front and rear ends of the upper surface of the support plate, and the outer ends of the guide plates are arc-shaped.
[0015] Compared with the prior art, the beneficial effects of this utility model are:
[0016] 1. This utility model, by setting up a base plate, vertical plate, support plate and coating structure, has the advantages of maintaining the height of the coating roller to coat the diluent, and is not affected by changes in height due to hand force. It solves the problem that the coating is not very uniform when manually using a sterile coating stick, as the coating is affected by hand force.
[0017] 2. This utility model has the advantage of positioning the culture medium plate by abutting the spring piece and the abutting groove after the culture medium plate is installed, thus maintaining the stability of the culture medium plate. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0019] Figure 2 This is a schematic diagram of the supporting structure of this utility model;
[0020] Figure 3 This is a schematic diagram of the coating structure connection of this utility model;
[0021] Figure 4 This is a schematic diagram of the connection structure of the aseptic coating roller of this utility model.
[0022] In the diagram: 100, base plate; 200, vertical plate; 300, support plate; 400, culture medium plate; 500, track; 600, coating structure;
[0023] 201. Shrapnel;
[0024] 301. Guide plate;
[0025] 401. Abutment groove;
[0026] 601. Moving plate; 602. Screw; 603. Rotary handle; 604. Connecting plate; 605. Buckle; 606. Aseptic coating roller; 607. Guide rod. Detailed Implementation
[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0028] This utility model provides two embodiments.
[0029] Example 1
[0030] Please see Figure 1 , Figure 3 and Figure 4 A highly efficient microbial community isolation and identification device includes a base plate 100, which is fixed on a workbench. Vertical plates 200 are fixedly installed on both sides of the upper surface of the base plate 100. Support plates 300 are fixedly installed on the bottom of opposite sides of the two vertical plates 200. The two support plates 300 are used to support a culture medium plate 400. Sample dilution solution is dropped onto the culture medium plate 400. The two support plates 300 are in contact with the lower surface of the culture medium plate 400 on both sides. Tracks 500 are fixedly installed on the top of opposite sides of the two vertical plates 200. The two tracks 500 are used to provide path guidance for the movement of the coating structure 600.
[0031] The coating structure 600 includes a movable plate 601 and a connecting plate 604. The two ends of the movable plate 601 slide along two tracks 500 respectively. The two ends of the tracks 500 are closed, so the movable plate 601 will not detach from the tracks 500. A screw 602 is threaded through the movable plate 601. A handle 603 is fixedly installed at the top of the screw 602. The connecting plate 604 is rotatably connected to the bottom end of the screw 602 through a rotating shaft. A sterile coating roller 606 is installed at the bottom of the connecting plate 604. A guide rod 607 is threaded through the movable plate 601. The bottom end of the guide rod 607 is fixedly connected to the connecting plate 604. Rotating the handle 603 causes the screw 602 to rotate and move relative to the moving plate 601 to adjust its height, thereby adjusting the height of the connecting plate 604. This adjusts the distance between the sterile coating roller 606 and the culture medium plate 400, allowing the diluent to be dripped onto the culture medium plate 400. This pushes the moving plate 601, connecting plate 604, and sterile coating roller 606 to move synchronously, thus applying the diluent through the sterile coating roller 606.
[0032] This utility model, by setting up a base plate 100, a vertical plate 200, a support plate 300, and a coating structure 600, has the advantages of a sterile coating roller 606 maintaining a constant height for coating the diluent, and the coating is not affected by changes in height due to hand force. It solves the problem that when using a sterile coating stick manually, the coating is not very uniform due to the force of the hand.
[0033] In this embodiment, the bottom ends of the connecting plate 604 are respectively provided with buckles 605, and the shafts at both ends of the aseptic coating roller 606 are respectively engaged with the two buckles 605. The aseptic coating roller 606 can be disassembled and replaced.
[0034] In this embodiment, the shafts at both ends of the aseptic coating roller 606 are rotatable relative to the two latches 605. When the aseptic coating roller 606 is moved, it can roll and coat the culture medium plate 400 upon contact.
[0035] Example 2
[0036] Please see Figure 2 A highly efficient device for isolating and identifying bacterial communities, wherein two vertical plates 200 are respectively provided with springs 201 on opposite sides, the springs 201 are arc-shaped, and the arched ends of the springs 201 abut against the grooves 401 opened on the side of the culture medium plate 400.
[0037] This invention, by setting up a spring piece 201 and an abutment groove 401, has the advantage that after the culture medium plate 400 is installed, the culture medium plate 400 is positioned by the abutment of the spring piece 201 and the abutment groove 401, thus maintaining the stability of the culture medium plate 400.
[0038] Guide plates 301 are fixedly installed at the front and rear ends of the upper surface of the support plate 300, and the outer end of the guide plate 301 is arc-shaped. When the culture medium plate 400 is pushed in laterally, the guide plate 301 can guide the culture medium plate 400 to be pushed in accurately. The arc shape of the outer end of the guide plate 301 forms a large opening, which is conducive to the pushing of the culture medium plate 400.
[0039] Working principle: A new culture medium plate 400 is pushed horizontally between two vertical plates 200. The culture medium plate 400 is pushed in between two guide plates 301 and moves along the support plate 300. During the pushing of the culture medium plate 400, the spring plate 201 is compressed until the arched section of the spring plate 201 corresponds to the abutment groove 401 and springs in. The culture medium plate 400 is positioned by the abutment between the spring plate 201 and the abutment groove 401. Rotating the handle 603 causes the screw 602 to rotate and move relative to the moving plate 601 to adjust its height, thereby driving the connecting plate 604 to adjust its height. This adjusts the distance between the sterile coating roller 606 and the culture medium plate 400, and the diluent is dripped onto the culture medium plate 400. This pushes the moving plate 601, the connecting plate 604, and the sterile coating roller 606 to move synchronously, spreading the diluent through the sterile coating roller 606. After separation, the culture medium plate 400 is removed and placed under a microscope for identification.
[0040] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.
Claims
1. A highly efficient device for isolating and identifying bacterial communities, characterized in that: Includes a base plate (100), on both sides of the upper surface of the base plate (100) are respectively provided with vertical plates (200), and support plates (300) are respectively provided at the bottom of the opposite side of the two vertical plates (200), the two support plates (300) are used to support the culture medium plate (400), and tracks (500) are respectively provided at the top of the opposite side of the two vertical plates (200), the two tracks (500) are used to provide a path guide for the movement of the coating structure (600); The coating structure (600) includes a movable plate (601) and a connecting plate (604). The two ends of the movable plate (601) slide along two tracks (500) respectively. A screw (602) is threaded through the movable plate (601). A handle (603) is provided at the top of the screw (602). The connecting plate (604) is rotatably connected to the bottom end of the screw (602). A sterile coating roller (606) is provided at the bottom of the connecting plate (604). A guide rod (607) is provided on the movable plate (601), and the bottom end of the guide rod (607) is fixedly connected to the connecting plate (604).
2. The device for separating and identifying high-efficiency bacterial flora according to claim 1, characterized in that: The bottom ends of the connecting plate (604) are respectively provided with buckles (605), and the shafts at both ends of the sterile coating roller (606) are respectively engaged with the two buckles (605).
3. The device for separating and identifying high-efficiency bacterial flora according to claim 2, characterized in that: The shafts at both ends of the sterile coating roller (606) are rotatable relative to the two latches (605).
4. The device for separating and identifying high-efficiency bacterial flora according to claim 1, characterized in that: The track (500) is closed at both ends.
5. The device for separating and identifying high-efficiency bacterial flora according to claim 1, characterized in that: Two vertical plates (200) are respectively provided with spring pieces (201) on opposite sides. The spring pieces (201) are arc-shaped, and the arched end of the spring pieces (201) abuts against the abutting groove (401) opened on the side of the culture medium plate (400).
6. The device for separating and identifying high-efficiency bacterial flora according to claim 5, characterized in that: The front and rear ends of the upper surface of the support plate (300) are respectively provided with guide plates (301), and the outer end of the guide plate (301) is arc-shaped.