A sperm cell counting test plate

Abstract

This invention provides a sperm cell counting detection plate, including a main frame. Auxiliary blocks are fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the bottom outer side of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. Secondary storage cylinders are fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame. This invention can achieve multiple sealing protection for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after semen is added, making subsequent observation and counting more convenient.

Description

Technical Field

[0001] This utility model relates to the field of medical testing tools, and in particular to a sperm cell counting test plate. Background Technology

[0002] In routine semen analysis and sperm function assessment, the sealing of the testing plate and the stability of the sample directly affect the accuracy of sperm counting. Current mainstream sperm counting instruments face the following technical bottlenecks:

[0003] 1. Sample leakage leads to counting errors.

[0004] Traditional counting chambers often employ an open slide structure, such as the counting chamber disclosed in patent CN201821567890.1. After the semen sample is added, it is simply covered with a coverslip, resulting in a leakage rate of 15%-20% due to shaking during transfer. Data from a reproductive medicine center shows that when using traditional counting chambers, the rate of duplicate testing due to sample leakage accounts for 12% of the total testing volume, with each duplicate test taking approximately 30 minutes, severely impacting testing efficiency.

[0005] 2. Insufficient sealing leads to sample contamination.

[0006] The existing counting chambers rely on manual pressing of the coverslip for sealing. Under a constant temperature of 37°C, the evaporation rate of samples due to seal failure reaches 8% per hour, and the deviation in sperm concentration detection exceeds 10%. In addition, the open structure is susceptible to contamination by external dust and microorganisms. Statistics from a hospital laboratory show that the invalidity rate of test results due to contamination reaches 5.6%.

[0007] 3. Poor adaptability to automated counting

[0008] Traditional counting chambers lack compatibility with CASA (Computer-Assisted Sperm Analysis System), such as the planar glass slide in patent CN202020345678.9, and cannot meet the system's precise requirements for sample layer thickness (ideal range 5-10 μm). Actual measurement data shows that the sample layer thickness deviation of traditional counting chambers reaches ±3 μm, resulting in an error rate exceeding 18% in sperm motility trajectory analysis.

[0009] Summary of technical pain points

[0010] Existing sperm counting and detection technologies suffer from three major problems: high risk of sample leakage, poor sealing stability, and insufficient automation. There is an urgent need for a new type of detection plate with multiple sealing protections, constant temperature and humidity, and standardized sample layers to achieve high-precision, low-error sperm counting and functional analysis. Utility Model Content

[0011] The purpose of this invention is to solve the problems existing in the above-mentioned background technology by proposing a sperm cell counting detection plate.

[0012] To achieve the above objectives, the present invention adopts the following technical solution:

[0013] A sperm cell counting detection plate includes a main frame, with auxiliary blocks fixed to the four corners of the outer side of the upper surface of the main frame. A cover plate is rotatably connected to the top rear end of the main frame via a hinge. A coverslip is fixed to the middle of the cover plate, and fixing blocks are fixed to the four corners of the outer side of the bottom of the cover plate. A glass slide is fixed to the middle of the main frame, and a main storage cylinder is fixed to the middle of the glass slide. A secondary storage cylinder is fixed to the left and right ends of the middle of the glass slide. An inner frame is fixed to the outer side of the inner frame, and a return spring and a contact plate are embedded inside the inner frame.

[0014] Preferably, the upper surface of the main frame, the glass slide, the inner frame, the main storage cylinder, and the secondary storage cylinder are all on the same horizontal plane.

[0015] Preferably, the depth of the main storage cylinder is greater than the depth of the secondary storage cylinder, the left and right ends of the main storage cylinder are connected to the interior of the secondary storage cylinder, and an anti-overflow groove is provided on the outer opening of the upper surface of the glass slide.

[0016] Preferably, the touch plate is a hollow rectangle with openings at the top and bottom, and the touch plate is embedded in a hidden groove inside the inner frame. The cross-sectional area of ​​the hidden groove is the same as that of the touch plate.

[0017] Preferably, the touch plate moves vertically up and down in the hidden groove of the inner frame, the return spring is fixedly connected between the touch plate and the inner frame, and the top of the touch plate extends from the top of the hidden groove.

[0018] Preferably, when the cover plate is tightly closed on the upper surface of the main frame, the middle part of the fixing block and the middle part of the auxiliary block are on the same vertical horizontal plane. The fixing block is made of permanent magnet material and magnetically adsorbs with the auxiliary block.

[0019] During the testing process, after the sperm is injected into the secondary storage cylinder, it flows from the secondary storage cylinder to the deeper main storage cylinder due to gravity. When the cover is tightened later, it covers the glass slide, sealing the top of both the main and secondary storage cylinders. At the same time, the cover pushes the contact plate down and hides it in the inner frame, compressing and deforming the return spring. Simultaneously, the return spring generates a rebound force, pushing the contact plate up to fit tightly against the cover plate. This achieves multiple sealing protections for the semen in the main storage cylinder, effectively preventing sperm leakage caused by device shaking after injection. This makes subsequent observation and counting more convenient. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a partial structural diagram of the main frame and inner frame in this utility model;

[0022] Figure 3 This is a schematic diagram of a partial front cross-sectional view of the main frame structure in this utility model;

[0023] Figure 4 This is a partial structural diagram of the main frame in this utility model.

[0024] Legend:

[0025] Main frame 1, auxiliary block 101, cover plate 2, cover glass 201, fixing block 202, glass slide 3, main storage cylinder 301, secondary storage cylinder 302, inner frame 4, return spring 401, touch plate 402. Detailed Implementation

[0026] Example 1, referring to Figure 1-4 A sperm cell counting detection plate includes a main frame 1. Auxiliary blocks 101 are fixed to the four corners of the outer side of the upper surface of the main frame 1. A cover plate 2 is rotatably connected to the top rear end of the main frame 1 via a hinge. A cover glass 201 is fixed to the middle of the cover plate 2. Fixing blocks 202 are fixed to the four corners of the bottom outer side of the cover plate 2. A glass slide 3 is fixed to the middle of the main frame 1. A main storage cylinder 301 is fixed to the middle of the glass slide 3. A secondary storage cylinder 302 is fixed to the left and right ends of the middle of the glass slide 3. An inner frame 4 is fixed to the inner side of the main frame 1. A return spring 401 and a touch plate 402 are embedded inside the inner frame 4.

[0027] The upper surface of the main frame 1, the glass slide 3, the inner frame 4, the main storage cylinder 301 and the secondary storage cylinder 302 are all on the same horizontal plane.

[0028] During the testing process, after the sperm is injected into the secondary storage cylinder 302, the sperm will flow from the secondary storage cylinder 302 to the deeper main storage cylinder 301 due to gravity. When the cover plate 2 is closed, the cover plate 2 and the cover glass 201 cover the glass slide 3, sealing the main storage cylinder 301 and the secondary storage cylinder 302 above the glass slide 3.

[0029] The depth of the main storage cylinder 301 is greater than the depth of the secondary storage cylinder 302. The left and right ends of the main storage cylinder 301 are connected to the interior of the secondary storage cylinder 302. An anti-overflow groove is provided on the outer opening of the upper surface of the glass slide 3.

[0030] The main function of the anti-overflow trough is to collect the semen flowing on the upper surface of the glass slide 3 when semen accidentally seeps out from the gap between the top of the main storage cylinder 301 and the secondary storage cylinder 302 and the cover plate 2.

[0031] Example 2 differs from Example 1 in that, in this example, the touch plate 402 is a hollow rectangle with openings at the top and bottom, and the touch plate 402 is precisely embedded in the hidden groove inside the inner frame 4. The cross-sectional area of ​​the hidden groove is the same as the cross-sectional area of ​​the touch plate 402.

[0032] The touch plate 402 moves vertically up and down in the hidden groove of the inner frame 4. The return spring 401 is fixedly connected between the touch plate 402 and the inner frame 4. The top of the touch plate 402 extends out from the top of the hidden groove.

[0033] At the same time, the cover plate 2 will push the touch plate 402 down to hide in the inner frame 4, squeezing the return spring 401 to compress and deform. At the same time, the return spring 401 will generate a rebound force to push the touch plate 402 up to stick to the cover plate, so as to achieve multiple sealing protection for the semen in the main storage cylinder 301. This can effectively prevent the semen from leaking due to the shaking of the device after the semen is added, and subsequent observation and counting will be more convenient.

[0034] When the cover plate 2 is tightly closed on the upper surface of the main frame 1, the middle part of the fixing block 202 and the middle part of the auxiliary block 101 are both on the same vertical horizontal plane. The fixing block 202 is made of permanent magnet material and is magnetically attracted to the auxiliary block 101.

[0035] When the cover plate 2 is tightly closed on the upper surface of the main frame 1, the fixing block 202 at the bottom of the cover plate 2 will attract the auxiliary block 101 on the upper surface of the main frame 1, so that the cover plate 2 is tightly closed on the upper surface of the main frame 1, preventing the cover plate 2 from accidentally opening from the upper surface of the main frame 1.

[0036] The above are merely preferred embodiments of this utility model. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of this utility model, and these should also be considered within the scope of protection of this utility model. These will not affect the implementation effect of this utility model or the practicality of the patent.

Claims

1. A sperm cell counting detection plate, comprising a main frame (1), characterized in that, Auxiliary blocks (101) are fixed to the four corners of the outer side of the upper surface of the main frame (1). The top rear end of the main frame (1) is rotatably connected to a cover plate (2) via a hinge. A cover glass (201) is fixed to the middle of the cover plate (2). Fixing blocks (202) are fixed to the four corners of the bottom outer side of the cover plate (2). A slide (3) is fixed to the middle of the main frame (1). A main storage cylinder (301) is fixed to the middle of the slide (3). A secondary storage cylinder (302) is fixed to the left and right ends of the middle of the slide (3). An inner frame (4) is fixed to the outer side of the inside of the main frame (1). A return spring (401) and a touch plate (402) are embedded inside the inner frame (4).

2. The sperm cell counting detection plate according to claim 1, characterized in that, The upper surface of the main frame (1), the glass slide (3), the inner frame (4), the main storage cylinder (301), and the upper surface of the auxiliary storage cylinder (302) are all on the same horizontal plane.

3. The sperm cell counting detection plate according to claim 1, characterized in that, The depth of the main storage cylinder (301) is greater than the depth of the secondary storage cylinder (302). The left and right ends of the main storage cylinder (301) are connected to the interior of the secondary storage cylinder (302). An anti-overflow groove is provided on the outer opening of the upper surface of the glass slide (3).

4. The sperm cell counting detection plate according to claim 1, characterized in that, The touch plate (402) is a hollow rectangle with openings at the top and bottom. The touch plate (402) is embedded in the hidden groove inside the inner frame (4). The cross-sectional area of ​​the hidden groove is the same as that of the touch plate (402).

5. The sperm cell counting detection plate according to claim 4, characterized in that, The touch plate (402) moves vertically up and down in the hidden groove of the inner frame (4), and the return spring (401) is fixedly connected between the touch plate (402) and the inner frame (4). The top of the touch plate (402) extends out from the top of the hidden groove.

6. The sperm cell counting detection plate according to claim 1, characterized in that, When the cover plate (2) is tightly closed on the upper surface of the main frame (1), the middle part of the fixing block (202) and the middle part of the auxiliary block (101) are both on the same vertical horizontal plane. The fixing block (202) is made of permanent magnet material and magnetically adsorbs with the auxiliary block (101).

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