A multichannel ELISA reaction plate for detecting antibodies against a feline triple vaccine.
By designing a multi-channel ELISA reaction plate, simultaneous detection of feline triple vaccine antibodies was achieved, solving the problems of cumbersome operation and high cost in existing technologies, improving detection efficiency and result accuracy, and making it suitable for rapid screening and automated detection of large-scale samples.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- 李权智
- Filing Date
- 2025-08-06
- Publication Date
- 2026-06-30
AI Technical Summary
Existing methods for detecting feline triple vaccine antibodies are cumbersome and time-consuming. Furthermore, the existing ELISA reaction plates are designed for single channels, which increases reagent consumption and sample volume when the three antibodies need to be detected separately, resulting in low detection efficiency, high cost, and poor reproducibility.
Design a multi-channel ELISA reaction plate containing FPV, FCV, and FHV reaction channels. Made of polystyrene, the channels are pre-coated with antigens, supporting simultaneous detection of three antibodies. It is compatible with automated equipment and features a sealing cap and snap-fit structure for easy storage and transportation.
It enables simultaneous detection of three antibodies, reduces operational steps and reagent usage, lowers result deviation, improves detection efficiency, is suitable for rapid screening of large-scale samples, reduces costs, adapts to automated processes, and reduces human error.
Smart Images
Figure CN224436335U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the technical field of multi-channel ELISA reaction plates for detecting feline triple vaccine antibodies, specifically a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies. Background Technology
[0002] Feline herpesvirus (FHV), feline calicivirus (FCV), and feline panleukopenia virus (FPV) are three major pathogens that endanger the health of felines, causing highly contagious diseases such as feline infectious rhinotracheitis, feline calicivirus infection, and feline panleukopenia, with particularly high mortality rates in kittens and immunocompromised cats. To effectively prevent these diseases, the feline triple vaccine has become a core component of routine immunization programs for pet cats, and its immunization efficacy directly affects the level of health protection for cats. Antibody titer testing is a key method for assessing vaccine efficacy. By measuring the levels of specific antibodies against FHV, FCV, and FPV in feline serum, the immune status of the body can be accurately determined, providing a scientific basis for adjusting subsequent vaccination strategies. Currently, commonly used antibody detection methods in clinical and laboratory settings include neutralization tests, indirect immunofluorescence assays, and traditional ELISA detection. Among these, the neutralization test is cumbersome, time-consuming, and requires highly skilled personnel, making it difficult to meet the needs of rapid detection of large-scale samples; the indirect immunofluorescence assay has problems such as strong subjectivity and poor reproducibility; although the traditional ELISA test has the advantages of simple operation and high sensitivity, most existing reaction plates are single-channel designs coated with a single antigen. If three antibodies need to be detected at the same time, different reaction plates must be used for independent experiments, which not only increases reagent consumption and sample volume, but may also lead to result deviations due to batch differences, reducing detection efficiency and increasing experimental costs. Utility Model Content
[0003] The purpose of this invention is to address the shortcomings of existing technologies by proposing a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies.
[0004] To achieve the above objectives, the present invention adopts the following technical solution: a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies, comprising a reaction plate body, the reaction plate body including a base plate, an ELISA reaction plate disposed on the base plate, an FPV reaction channel, an FCV reaction channel and an FHV reaction channel disposed on the ELISA reaction plate, and an ELISA plate sealing cap snapped onto the ELISA reaction plate.
[0005] As a further description of the above technical solution:
[0006] The ELISA reaction plate is made of polystyrene, and the FPV reaction channel, FCV reaction channel and FHV reaction channel are parallel to each other.
[0007] As a further description of the above technical solution:
[0008] The FPV reaction channel is composed of several groups of uniformly distributed pre-coated FPV antigen channels, the FCV reaction channel is composed of several groups of uniformly distributed pre-coated FCV antigen channels, and the FHV reaction channel is composed of several groups of uniformly distributed pre-coated FHV antigen channels.
[0009] As a further description of the above technical solution:
[0010] The ELISA reaction plate is snapped into the snap-fit groove on the inner side of the ELISA plate sealing cover by a snap-fit block fixed on the outside.
[0011] As a further description of the above technical solution:
[0012] The base plate is connected to a snap-fit block fixed on the outside of another set of ELISA reaction plates via a snap-fit groove provided on the inner side of the bottom.
[0013] As a further description of the above technical solution:
[0014] A groove is provided on one side of the base plate.
[0015] This utility model has the following beneficial effects:
[0016] 1. With three sets of parallel reaction channels pre-coated with FPV, FCV, and FHV antigens respectively, the simultaneous detection of three antibodies in a single sample can be achieved, thereby greatly reducing experimental operation steps and avoiding repeated sample addition and batch reactions in single-channel detection. This not only significantly saves reagent consumption and sample consumption, but also effectively reduces the result deviation caused by experimental batch differences, significantly improving detection efficiency, and is especially suitable for rapid screening of large-scale samples.
[0017] 2. The main body of the reaction plate is made of polystyrene material, which is compatible with standard enzyme-linked immunosorbent assay (ELISA) readers and automatic samplers. It is adapted to automated experimental procedures, reducing human error. At the same time, the enclosed and stackable structure facilitates sample preservation and contamination control during the experiment, while saving storage space and transportation costs, enhancing its practicality and applicability in multiple scenarios such as animal experiments, vaccine evaluation, and clinical sample testing. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of a multichannel ELISA reaction plate for detecting feline triple vaccine antibodies proposed in this utility model;
[0019] Figure 2 This is a schematic diagram of the channel distribution of a multichannel ELISA reaction plate for detecting feline triple vaccine antibodies proposed in this utility model;
[0020] Figure 3 This is a bottom view of a multichannel ELISA reaction plate for detecting feline triple vaccine antibodies proposed in this utility model.
[0021] Legend:
[0022] 1. Base plate; 2. ELISA plate sealing cap; 3. ELISA reaction plate; 4. Snap-fit block; 5. Snap-fit slot one; 6. Snap-fit slot two; 7. FPV reaction channel; 8. FCV reaction channel; 9. FHV reaction channel; 10. Groove. Detailed Implementation
[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0024] Example 1:
[0025] like Figures 1 to 3 As shown in the figure, this embodiment provides a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies, including a reaction plate body, the reaction plate body including a base plate 1, an ELISA reaction plate 3 disposed on the base plate 1, an FPV reaction channel 7, an FCV reaction channel 8 and an FHV reaction channel 9 disposed on the ELISA reaction plate 3, and an ELISA plate sealing cap 2 snapped onto the ELISA reaction plate 3.
[0026] Understandable Figure 1 This illustration only schematically shows some components of a multichannel ELISA reaction plate for detecting feline triple vaccine antibodies. The actual shape, size, location, and construction of these components are not subject to change. Figure 1 The limitations of a feline triple vaccine antibody detection multichannel ELISA reaction plate can also include, compared to Figure 1 More or fewer parts.
[0027] In this embodiment, the base plate 1 provides stable support for the entire structure within the reaction plate body. The FPV reaction channel 7, FCV reaction channel 8, and FHV reaction channel 9 on the ELISA reaction plate 3 can simultaneously perform parallel reactions of three antibodies. The ELISA plate sealing cap 2 snaps into the ELISA reaction plate 3, preventing sample or reagent evaporation and contamination, and ensuring a stable reaction environment. The multi-channel setup enables simultaneous detection of the three antibodies in the feline triple vaccine, eliminating the need for separate operations and significantly improving experimental efficiency. The ELISA plate sealing cap 2 ensures that the reaction system is not affected by external interference, improving the accuracy of the detection results, making it suitable for various scenarios such as animal experiments and vaccine evaluation.
[0028] Specifically, the main body of the ELISA reaction plate is made of polystyrene, and the FPV reaction channel 7, FCV reaction channel 8 and FHV reaction channel 9 are parallel to each other.
[0029] In this embodiment, the polystyrene reaction plate body has good chemical stability and light transmittance, making it suitable for antigen-antibody binding and colorimetric observation in ELISA reactions. The three parallel reaction channels operate independently, specifically binding to FPV, FCV, and FHV antibodies in the sample respectively without interference. Simultaneously, the polystyrene material is compatible with standard ELISA readers, ensuring stable reading of the colorimetric signal; the parallel channel setup avoids cross-contamination and allows for simultaneous acquisition of detection data for the three antibodies, saving reagent consumption and experimental time.
[0030] Specifically, FPV reaction channel 7 consists of several groups of uniformly distributed pre-coated FPV antigen channels, FCV reaction channel 8 consists of several groups of uniformly distributed pre-coated FCV antigen channels, and FHV reaction channel 9 consists of several groups of uniformly distributed pre-coated FHV antigen channels.
[0031] As a preferred implementation, the channels of FPV reaction channel 7 are pre-coated with FPV antigen, which can specifically bind to FPV antibodies in the sample; similarly, FCV reaction channels 8 and FHV reaction channels 9 capture the corresponding antibodies through the pre-coated FCV and FHV antigens, respectively. Multiple channels in each group can be used for repeated experiments, reducing errors; the pre-coated antigen eliminates the need for the pre-experiment coating step, simplifying the operation; and the multiple channel setup improves the reliability of the detection results, meeting the accuracy requirements of sample testing.
[0032] Specifically, the ELISA reaction plate 3 is snapped into the snap-fit groove 5 on the inner side of the ELISA plate sealing cover 2 by the snap-fit block 4 fixed on the outside.
[0033] It should be noted that the snap-fit block 4 on the outside of the ELISA reaction plate 3 matches the snap-fit groove 5 on the inside of the ELISA plate sealing cap 2. When closed, the snap-fit block 4 is embedded in the snap-fit groove 5 to form a tight seal. When opening, the ELISA plate sealing cap 2 can be separated by simply pushing it upwards, which is convenient. The snap-fit structure ensures a stable fit between the ELISA plate sealing cap 2 and the ELISA reaction plate 3, effectively preventing liquid leakage or the entry of external contaminants during the reaction. At the same time, it facilitates quick opening and closing, improving the efficiency of experimental operations.
[0034] Specifically, the base plate 1 is connected to the snap-fit block 4 fixed on the outside of another set of ELISA reaction plates 3 via the snap-fit groove 2 6 set on the inner side of the bottom.
[0035] As a preferred embodiment, the snap-fit groove 6 on the inner side of the bottom of the base plate 1 can engage with the snap-fit block 4 on the outer side of another set of ELISA reaction plates 3, allowing multiple sets of reaction plates to be stacked vertically, saving storage and transportation space. The stacking arrangement improves space utilization, facilitates laboratory storage and long-distance transportation, and the snap-fit structure prevents sliding and tipping during stacking, protecting the ELISA reaction plates 3 and the internal samples.
[0036] Example 2:
[0037] Specifically, a groove 10 is provided on one side of the base plate 1.
[0038] It should be noted that the groove 10 on one side of the base plate 1 provides a point of leverage for the fingers, making it easy to open the ELISA reaction plate 3 and the ELISA plate sealing cover 2, as well as the base plate 1 and another set of ELISA reaction plates 3.
[0039] When using this product, the sample is added to the three channels: the FPV reaction channel, the FCV reaction channel, and the FHV reaction channel. This allows for the simultaneous binding and colorimetric reaction of antibodies against these three viruses with their corresponding antigens. The results are then read using an ELISA reader, and the antibody titer is calculated.
[0040] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A multichannel ELISA reaction plate for detecting feline triple vaccine antibodies, characterized in that: The reaction plate includes a main body, which includes a base plate (1). An ELISA reaction plate (3) is disposed on the base plate (1). An FPV reaction channel (7), an FCV reaction channel (8) and an FHV reaction channel (9) are disposed on the ELISA reaction plate (3). An ELISA plate sealing cap (2) is snapped onto the ELISA reaction plate (3).
2. The multichannel ELISA reaction plate for detecting feline triple vaccine antibodies according to claim 1, characterized in that: The main body of the ELISA reaction plate is made of polystyrene, and the FPV reaction channel (7), FCV reaction channel (8) and FHV reaction channel (9) are parallel to each other.
3. The multichannel ELISA reaction plate for detecting feline triple vaccine antibodies according to claim 2, characterized in that: The FPV reaction channel (7) is composed of several groups of uniformly distributed pre-coated FPV antigen channels, the FCV reaction channel (8) is composed of several groups of uniformly distributed pre-coated FCV antigen channels, and the FHV reaction channel (9) is composed of several groups of uniformly distributed pre-coated FHV antigen channels.
4. The multichannel ELISA reaction plate for detecting feline triple vaccine antibodies according to claim 1, characterized in that: The ELISA reaction plate (3) is snapped into the snap-fit groove (5) set on the inner side of the ELISA plate sealing cover (2) by the snap-fit block (4) fixed on the outer side.
5. A multichannel ELISA reaction plate for detecting feline triple vaccine antibodies according to claim 1, characterized in that: The base plate (1) is connected to the snap-fit block (4) fixed on the outside of another set of ELISA reaction plates (3) through the snap-fit groove (6) provided on the inner side of the bottom.
6. The multichannel ELISA reaction plate for detecting feline triple vaccine antibodies according to claim 5, characterized in that: A groove (10) is provided on one side of the base plate (1).