A microbial culture device

Abstract

This utility model discloses a microbial culture device, including a housing, an oscillation mechanism, and a culture mechanism. The housing has a culture chamber and a mounting chamber. The oscillation mechanism includes a driving component and a limiting component. The limiting component is slidably disposed within the culture chamber, and the driving component is disposed within the mounting chamber. The output end of the driving component extends into the culture chamber and connects to the limiting component. The driving component is used to drive the limiting component to reciprocate. The culture mechanism is detachably connected to the limiting component. The culture mechanism has a cavity for holding microbial culture medium. This invention solves the problem in the prior art where uneven distribution of nutrients during microbial culture easily leads to growth inhibition and metabolic abnormalities.

Description

Technical Field

[0001] This utility model relates to the field of microbial culture technology, specifically to a microbial culture device. Background Technology

[0002] Microorganisms are a collective term for all tiny organisms that are difficult to observe with the naked eye, including bacteria, viruses, fungi, and a few algae. In the process of culturing microorganisms, they are usually inoculated onto a culture medium (depending on the specific needs of the microorganisms, they may be selectively placed in culture flasks or petri dishes), and then placed in an incubator for cultivation.

[0003] In the existing patent document CN211420132U, a device for microbial culture is disclosed, specifically including a main board, a base, a support plate, a notch, a cavity, a connecting component, and a crossbar. The support plate has a fixing box, which has a receiving cavity, a box opening, a sealing rubber gasket, and a box cover. The connecting component includes a connecting rod and a telescopic hose. With the above scheme, microorganisms are placed in the fixing box for culture. If the nutrient distribution is uneven, the densely populated areas of microorganisms rapidly consume carbon and nitrogen sources, forming "starvation zones," leading to cell growth stagnation (e.g., a 50%-80% decrease in bacterial division rate). Waste such as organic acids and ammonium ions accumulate locally, causing a sudden drop in pH (possibly below 5.0), inhibiting enzyme activity. This results in significant differences in cell density within the culture system, with some areas experiencing microbial death and others overgrowth. Utility Model Content

[0004] The purpose of this invention is to overcome the above-mentioned technical deficiencies and provide a microbial culture device that solves the problem of uneven nutrient distribution and easy growth inhibition and metabolic abnormalities during microbial culture in the prior art.

[0005] To achieve the above-mentioned technical objectives, the present invention adopts the following technical solution:

[0006] This utility model provides a microbial culture device, including

[0007] The box body contains a culture chamber and an installation chamber;

[0008] An oscillation mechanism, comprising a driving member and a limiting member, wherein the limiting member is slidably disposed within the culture chamber, and the driving member is disposed within the mounting cavity, with its output end extending into the culture chamber and connected to the limiting member, the driving member being used to drive the limiting member to reciprocate; and...

[0009] A culture mechanism, wherein the culture mechanism is detachably connected to the limiting member, and the culture mechanism has a cavity for holding microbial culture medium.

[0010] In one embodiment, the driving component includes a geared motor, a connecting rod, a transmission rod, a guide rod, and a sleeve. The geared motor is fixedly disposed in the mounting cavity. The output end of the geared motor is fixedly connected to one end of the connecting rod. The other end of the connecting rod is rotatably connected to one end of the transmission rod. The other end of the transmission rod is rotatably connected to one end of the guide rod. The other end of the guide rod passes through the mounting cavity and is connected to a limiting component. The sleeve is fixedly disposed in the culture cavity and slidably sleeved with the guide rod.

[0011] In one embodiment, the limiting member includes a seat and a locking part. The seat has a limiting groove. The seat is slidably installed in the culture chamber and connected to the other end of the guide rod. The locking part is connected to the seat. When the culture mechanism is placed in the limiting groove, the locking part has a first state of abutting against the culture mechanism and a second state of being separated from the culture mechanism. In the first state, the culture mechanism is fixed in the limiting groove. In the second state, the culture mechanism can be moved out of the limiting groove.

[0012] In one embodiment, the locking part includes at least one locking screw, which is threadedly rotatably connected to the seat body and can extend into the limiting groove.

[0013] In one embodiment, the culture mechanism includes a placement box with a cavity inside, and sliders are respectively provided on opposite sides of the placement box. The base is provided with two slots. When the placement box is located in the limiting groove, the two sliders are respectively inserted into the two slots.

[0014] In one embodiment, the locking screw can extend into the slot and abut against or separate from the slider.

[0015] In one embodiment, the placement box is provided with multiple positioning sleeves for limiting the position of the culture tube.

[0016] In one embodiment, the inner wall of the positioning sleeve is provided with an elastic washer.

[0017] In one embodiment, a light-illuminating unit is disposed inside the culture chamber and above the seat.

[0018] In one embodiment, an air exchange unit is installed on the box corresponding to the culture chamber.

[0019] Compared with the prior art, the microbial culture device provided by this utility model has a culture chamber and an installation chamber in the box. The limiting member is slidably disposed in the culture chamber and the driving member is disposed in the installation chamber. The output end of the driving member extends into the culture chamber and is connected to the limiting member. When the culture mechanism is connected to the limiting member, the driving member can drive the limiting member to reciprocate, thereby causing the microbial culture medium in the culture mechanism to oscillate slightly. This allows the nutrients, oxygen and metabolites in the culture mechanism to be continuously mixed, avoids local concentration differences, and prevents the aggregation and precipitation of microbial cells, ensuring that the cells are evenly distributed in the culture mechanism. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the structure of a microbial culture device provided in an embodiment of this utility model;

[0021] Figure 2 yes Figure 1 Enlarged view of region A in the middle;

[0022] Figure 3 yes Figure 1 Enlarged view of region B in the middle;

[0023] Figure 4 This is a schematic diagram of the structure of the cultivation mechanism provided in this embodiment of the utility model;

[0024] Figure 5 This is a schematic diagram of the structure of the limiting member provided in this embodiment of the utility model. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining this utility model and are not intended to limit this utility model.

[0026] This invention addresses the technical problem in existing technologies where uneven nutrient distribution during microbial culture leads to growth inhibition and metabolic abnormalities. It provides a microbial culture device that can drive the microbial culture medium within the culture chamber to vibrate slightly, allowing nutrients, oxygen, and metabolic products to mix continuously, avoiding local concentration differences, preventing microbial cell aggregation and precipitation, and ensuring uniform cell distribution within the culture chamber.

[0027] Please see Figures 1-5 , Figures 1-5A microbial culture device according to one embodiment of the present invention includes a housing 1, an oscillation mechanism 2, and a culture mechanism 3. The housing 1 is provided with a culture chamber 1a and a mounting chamber 1b. The oscillation mechanism 2 includes a driving member 21 and a limiting member 22. The limiting member 22 is slidably disposed in the culture chamber 1a, and the driving member 21 is disposed in the mounting chamber 1b. The output end of the driving member 21 extends into the culture chamber 1a and is connected to the limiting member 22. The driving member 21 is used to drive the limiting member 22 to reciprocate. The culture mechanism 3 is detachably connected to the limiting member 22, and the culture mechanism 3 has a cavity for holding microbial culture medium.

[0028] In actual use, by placing microorganisms and microbial culture medium into the cavity of the culture mechanism 3, connecting the culture mechanism 3 to the limiting member 22, and driving the limiting member 22 to reciprocate through the driving member 21, the culture mechanism 3 can be driven to oscillate slightly, ensuring that the nutrients, oxygen and metabolic products in the cavity can be continuously mixed, avoiding local concentration differences, and preventing the aggregation and precipitation of microbial cells.

[0029] Understandably, the driving component 21 drives the reciprocating motion of the limiting component 22, and the motion is relatively slow, just enough to make the culture mechanism 3 oscillate slightly; specifically, the reciprocating motion of the limiting component 22 takes 2-4 seconds for one cycle, so as to avoid the culture mechanism 3 vibrating too much, which would cause the microorganisms and microbial culture solution inside the culture mechanism 3 to splash out.

[0030] It should be noted that the box 1 is provided with partitions, and multiple partitions divide the interior of the box 1 into multiple culture chambers 1a and multiple installation chambers 1b; in other embodiments, multiple oscillation mechanisms 2 are spaced apart along the height direction of multiple installation chambers 1b, wherein multiple support plates are spaced apart along the height direction in the installation chamber 1b, and each of the oscillation mechanisms 2 is respectively installed on each support plate; multiple bottom plates are spaced apart along the height direction in the culture chamber 1a, and each of the limiting members 22 is slidably connected to each bottom plate, so that the microbial culture device can simultaneously place multiple culture mechanisms 3.

[0031] In addition, the housing 1 is equipped with a dark door, which can open or close the culture chamber 1a and the installation chamber 1b.

[0032] It should be noted that the driving component is not limited to a specific structure, as long as it can drive the limiting component 22 to slide back and forth, and no further details will be provided here.

[0033] In one embodiment, the driving component 21 includes a geared motor 211, a connecting rod 212, a transmission rod 213, a guide rod 214, and a sleeve 215. The geared motor 211 is fixedly disposed in the mounting cavity 1b. The output end of the geared motor 211 is fixedly connected to one end of the connecting rod 212. The other end of the connecting rod 212 is rotatably connected to one end of the transmission rod 213. The other end of the transmission rod 213 is rotatably connected to one end of the guide rod 214. The other end of the guide rod 214 passes through the mounting cavity 1b and is connected to the limiting component 22. The sleeve 215 is fixedly disposed in the culture cavity 1a and is slidably sleeved with the guide rod 214.

[0034] It is understandable that by driving the connecting rod 212 to rotate through the geared motor 211, the transmission rod 213 can be driven to rotate, thereby driving the guide rod 214 to slide relative to the sleeve 215, and causing the limiting member 22 to reciprocate.

[0035] It should be noted that, in one embodiment, the limiting member 22 includes a seat 221 and a locking part 222. The seat 221 has a limiting groove 221a. The seat 221 is slidably installed in the culture chamber 1a and connected to the other end of the guide rod 214. The locking part 222 is connected to the seat 221. When the culture mechanism 3 is placed in the limiting groove 221a, the locking part 222 has a first state of abutting against the culture mechanism 3 and a second state of being separated from the culture mechanism 3. In the first state, the culture mechanism 3 is fixed in the limiting groove 221a. In the second state, the culture mechanism 3 can be moved out of the limiting groove 221a.

[0036] Understandably, after the culture mechanism 3 is fixedly connected to the seat 221, the culture mechanism 3 can reciprocate with the seat 221. The culture mechanism 3 has good stability and can avoid relative sliding between the culture mechanism 3 and the seat 221.

[0037] In this specific embodiment, the locking part 222 includes at least one locking screw, which is threadedly rotatably connected to the seat 221, and the locking screw can extend into the limiting groove 221a.

[0038] In one embodiment, the culture mechanism 3 includes a placement box 31 with a cavity inside, and sliders 311 are respectively provided on opposite sides of the placement box 31. The seat 221 is provided with two slots 221b. When the placement box 31 is located in the limiting groove 221a, the two sliders 311 are respectively inserted into the two slots 221b.

[0039] Specifically, the locking screw can extend into the slot 221b and abut or separate from the slider 311; when the locking screw abuts against the slider 311, it can fix the placement box 31; when the locking screw separates from the slider 311, it can push the placement box 31 to move relative to the seat 221.

[0040] Based on the above scheme, in order to place the culture tube in the placement box 31, specifically, the placement box 31 is provided with a plurality of positioning sleeves 312 for limiting the culture tube. It should be noted that the outer diameter of the culture tube is the same as the inner diameter of the positioning sleeve 312.

[0041] In addition, in other embodiments, the inner wall of the positioning sleeve 312 is provided with an elastic washer. When the culture tube is inserted into the positioning sleeve 312, the positioning sleeve 312 can generate elastic deformation and apply a pre-tightening force to the culture tube.

[0042] Based on the above scheme, a light-emitting unit 4 is provided in the culture chamber 1a and above the seat 221; it should be noted that phototrophic microorganisms (such as photosynthetic bacteria and cyanobacteria) need light to perform photosynthesis to synthesize organic matter; and the light intensity usually needs to be 2000-10000 lux (for example, photosynthetic bacteria grow best at 5000-10000 Lx).

[0043] Based on the above scheme, an air exchange unit 5 is installed on the box 1 corresponding to the culture chamber 1a. It can be understood that multiple ventilation holes are opened on the bottom plate, and multiple ventilation holes connected to the culture chamber 1a are provided on the box. The air exchange unit 5 can accelerate the air flow in the culture chamber 1a. For example, bacteria (Escherichia coli) and actinomycetes rely on oxygen for aerobic respiration, and ventilation ensures a sufficient oxygen supply. In addition, CO2 and volatile organic compounds generated during the culture process need to be discharged in time to prevent growth inhibition.

[0044] The specific embodiments of this utility model described above do not constitute a limitation on the scope of protection of this utility model. Any other corresponding changes and modifications made based on the technical concept of this utility model should be included within the scope of protection of the claims of this utility model.

Claims

1. A microbial culture device, characterized by, include The box body contains a culture chamber and an installation chamber; An oscillation mechanism is provided, comprising a driving member and a limiting member. The limiting member is slidably disposed within the culture chamber, and the driving member is disposed within the mounting cavity. The output end of the driving member extends into the culture chamber and is connected to the limiting member. The driving member is used to drive the limiting member to reciprocate. as well as, A culture mechanism, wherein the culture mechanism is detachably connected to the limiting member, and the culture mechanism has a cavity for holding microbial culture medium.

2. The microorganism culture device according to claim 1, wherein The driving component includes a geared motor, a connecting rod, a transmission rod, a guide rod, and a sleeve. The geared motor is fixedly installed in the mounting cavity. The output end of the geared motor is fixedly connected to one end of the connecting rod. The other end of the connecting rod is rotatably connected to one end of the transmission rod. The other end of the transmission rod is rotatably connected to one end of the guide rod. The other end of the guide rod passes through the mounting cavity and is connected to a limiting component. The sleeve is fixedly installed in the culture cavity and is slidably sleeved with the guide rod.

3. The device of claim 2, wherein the device is a microbe culture device. The limiting component includes a seat and a locking part. The seat has a limiting groove. The seat is slidably installed in the culture chamber and connected to the other end of the guide rod. The locking part is connected to the seat. When the culture mechanism is placed in the limiting groove, the locking part has a first state of abutting against the culture mechanism and a second state of being separated from the culture mechanism. In the first state, the culture mechanism is fixed in the limiting groove. In the second state, the culture mechanism can be moved out of the limiting groove.

4. The microorganism culture device according to claim 3, wherein The locking part includes at least one locking screw, which is threadedly rotatably connected to the seat body, and the locking screw can extend into the limiting groove.

5. The device of claim 4, wherein the device is a microbe culture device. The culture mechanism includes a placement box with a cavity inside, and sliders are respectively provided on opposite sides of the placement box. The base is provided with two slots. When the placement box is located in the limiting groove, the two sliders are respectively inserted into the two slots.

6. The device of claim 5, wherein the device is a microbe culture device. The locking screw can extend into the slot and abut against or separate from the slider.

7. The device of claim 6, wherein the device is a microbe culture device. The placement box is equipped with multiple positioning sleeves for limiting the position of the culture tubes.

8. A microbial culture device according to claim 7, characterized in that, The inner wall of the positioning sleeve is provided with an elastic washer.

9. The microorganism culture device according to claim 3, wherein A light-illuminating unit is provided inside the culture chamber and above the base.

10. The device of claim 1, wherein An air exchange unit is installed on the box corresponding to the culture chamber.

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