A stem cell expansion and culture device

Abstract

This utility model relates to the field of cell culture technology, and in particular to a stem cell expansion and culture device, including a heatable incubator. A viewing door is movably installed on the front left side of the heatable incubator. A display screen and operation keys are arranged sequentially from back to front on the upper end of the heatable incubator. A uniform heating device is fixedly connected to the middle of the lower end of the heatable incubator, with its upper part located inside the heatable incubator. This stem cell expansion and culture device provides a base temperature through the heatable incubator, ensures uniform heating of the culture dish assembly through the uniform heating device, and features a vibrating and shaking component that, in conjunction with a vibrating component, shakes the upper culture dish to promote thorough mixing and contact between stem cells and the culture medium. The viewing door facilitates observation, and the display screen and operation keys allow for easy control. Overall, it improves the efficiency and quality of stem cell expansion, making operation convenient and suitable for the field of cell culture.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a stem cell expansion and culture device. Background Technology

[0002] Stem cells have significant value in regenerative medicine and other fields due to their self-renewal and multi-directional differentiation potential. Their clinical applications and basic research require a large number of high-quality stem cells. However, the source of natural stem cells is limited, and in vitro culture is easily affected by the environment, leading to differentiation and reduced activity. Therefore, a dedicated expansion culture device is required. However, the existing expansion culture device has the following shortcomings: First, the microenvironment of the culture environment is not uniform, and the distribution of nutrients is uneven, resulting in different cell proliferation rates and phenotypic heterogeneity. Second, traditional adherent stem cell culture relies on multi-layer culture flasks, which is cumbersome to operate and prone to errors due to manual operation. Utility Model Content

[0003] The main objective of this invention is to provide a stem cell expansion and culture device that can effectively solve the problems in the background art.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] A stem cell expansion and culture device includes a heatable incubator. A viewing door is movably installed on the left front end of the heatable incubator. A display screen and operation keys are arranged sequentially from back to front on the upper end of the heatable incubator. A uniform heating device is fixedly connected to the middle of the lower end of the heatable incubator, and the upper part of the uniform heating device is located inside the heatable incubator.

[0006] Preferably, the uniform heating device includes a driver, the output end of which passes through the lower middle part of the heatable incubator and is fixedly connected to a bottom frame, a culture dish assembly is inserted into the bottom frame, and a fixing rod is fixedly connected to the upper left and upper right parts of the bottom frame, and a vibrating shaking assembly is fixedly connected to the upper parts of the two fixing rods.

[0007] Preferably, the culture dish assembly includes a culture box, and a handle block is fixedly connected to both the front and rear of the outer surface of the culture box. Several culture dish bodies are placed inside the culture box.

[0008] Preferably, the diameter area of ​​the culture box is equal to the inner diameter area of ​​the bottom frame, and the culture box is located inside the bottom frame.

[0009] Preferably, the vibrating and shaking component includes a U-shaped frame, with springs fixedly connected to the four corners of the lower inner wall of the U-shaped frame, and a placement frame fixedly connected to the ends of the four springs away from the U-shaped frame. A placement groove is provided at the upper end of the placement frame, and an upper petri dish is inserted into the placement groove. Fixed blocks are fixedly connected to the lower left and lower right ends of the U-shaped frame, and a vibration component is embedded in the middle of the inner left wall of the U-shaped frame.

[0010] Preferably, the square frame is fixedly connected to the upper part of the two fixed rods by two fixed blocks, and the upper culture dish component and the culture dish assembly are the same in size and structure.

[0011] Preferably, the vibration component includes a servo motor, which is embedded in the middle of the inner left wall of the U-shaped frame. The output end of the servo motor is fixedly connected to a connecting rod, and the right end of the connecting rod is movably connected to the U-shaped frame through a bearing. Cam blocks are fixedly connected to both the left and right outer surfaces of the connecting rod.

[0012] Preferably, the two cam blocks are arranged in a left-right mirror image distribution, the two cam blocks do not contact the inner wall of the square frame, and the two cam blocks are located together below the placement frame.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. In this utility model, the driver drives the bottom frame to rotate, causing the internal culture dish assembly to rotate accordingly, achieving uniform heating. The culture box of the culture dish assembly can accommodate multiple culture dishes. The handle block is convenient for picking up and putting down, and its fit with the bottom frame ensures stable rotation. The fixing rod can limit the vibration and shaking component, ensuring that it works in tandem with the culture dish assembly. All accessories work together to improve heating uniformity, facilitate batch culture operations, provide a stable growth environment for stem cells, and help improve expansion efficiency.

[0015] 2. In this utility model, the U-shaped frame provides an installation base for each component, the fixed plug securely connects it to the fixed rod, the servo motor drives the connecting rod to rotate, the cam block rotates accordingly to lift the placement frame, the spring cooperates to make the placement frame vibrate up and down, the upper culture dish in the placement slot shakes synchronously, all the accessories cooperate, and the vibration allows the stem cells to fully contact the culture medium, promotes growth, and the structure is stable, ensuring uniform vibration, improving the amplification effect, and facilitating batch culture operations. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of a stem cell expansion and culture device according to the present invention;

[0017] Figure 2 This is a schematic diagram of the overall structure of the uniform heating device of the stem cell expansion and culture apparatus of this utility model;

[0018] Figure 3 This is a schematic diagram of the overall structure of the culture dish assembly of a stem cell expansion culture device according to the present invention;

[0019] Figure 4 This is a schematic diagram of the overall structure of the vibrating and shaking component of a stem cell expansion and culture device according to the present invention.

[0020] Figure 5 This is a schematic diagram of the overall structure of the vibration component of a stem cell expansion and culture device according to the present invention.

[0021] In the diagram: 1. Heated incubator; 2. Viewing door; 3. Display screen; 4. Operation keys; 5. Uniform heating device; 51. Driver; 52. Base frame; 53. Fixing rod; 54. Petri dish assembly; 55. Vibrating and shaking assembly; 541. Culture box; 542. Handle block; 543. Petri dish body; 551. U-shaped frame; 552. Spring; 553. Placement frame; 554. Placement slot; 555. Upper petri dish component; 556. Vibrating component; 557. Fixing block; 5561. Servo motor; 5562. Connecting rod; 5563. Cam block. Detailed Implementation

[0022] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0023] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0024] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0025] Please see Figure 1-5This utility model provides a technical solution:

[0026] A stem cell expansion and culture device includes a heatable incubator 1. A viewing door 2 is movably installed on the left front end of the heatable incubator 1. A display screen 3 and operation keys 4 are arranged sequentially from back to front on the upper end of the heatable incubator 1. A uniform heating device 5 is fixedly connected to the middle of the lower end of the heatable incubator 1. The upper part of the uniform heating device 5 is located inside the heatable incubator 1.

[0027] In this embodiment, the uniform heating device 5 includes a driver 51. The output end of the driver 51 passes through the lower middle part of the heatable incubator 1 and is fixedly connected to a bottom frame 52. A petri dish assembly 54 is inserted into the bottom frame 52. A fixing rod 53 is fixedly connected to the upper left and upper right parts of the bottom frame 52. A vibrating and shaking component 55 is fixedly connected to the upper parts of the two fixing rods 53. The petri dish assembly 54 includes a culture box 541. A handle block 542 is fixedly connected to the front and rear parts of the outer surface of the culture box 541. A plurality of petri dish bodies 543 are placed inside the culture box 541. The diameter area of ​​the culture box 541 is equal to the inner diameter area of ​​the bottom frame 52. The culture box 541 is located inside the bottom frame 52.

[0028] Through the above scheme: the driver 51 drives the bottom frame 52 to rotate, which in turn drives the internal culture dish assembly 54 to rotate. The culture dish 543 inside the culture box 541 rotates with it. At the same time, the vibration and shaking assembly 55 operates. Combined with the heating of the uniform heating device 5, the nutrient concentration and temperature inside the culture dish 543 are more uniform. The components work together to improve the uniformity of the microenvironment, reduce the difference in cell proliferation rate and phenotypic heterogeneity. In addition, the culture box 541 is compatible with the bottom frame 52, and the handle block 542 makes it easy to pick up and put down, making the operation convenient.

[0029] In this embodiment, the vibrating and shaking component 55 includes a U-shaped frame 551. Springs 552 are fixedly connected to the four corners of the lower inner wall of the U-shaped frame 551. A placement frame 553 is fixedly connected to the ends of the four springs 552 away from the U-shaped frame 551. A placement groove 554 is provided at the upper end of the placement frame 553, and an upper petri dish component 555 is inserted into the placement groove 554. Fixing blocks 557 are fixedly connected to the lower left and lower right ends of the U-shaped frame 551. A vibrating component 556 is embedded in the middle of the inner left wall of the U-shaped frame 551. The U-shaped frame 551 is fixedly connected to the upper parts of two fixing rods 53 via two fixing blocks 557. The size and structure of component 555 are the same as those of the petri dish assembly 54. The vibration component 556 includes a servo motor 5561, which is embedded in the middle of the inner left wall of the U-shaped frame 551. The output end of the servo motor 5561 is fixedly connected to a connecting rod 5562. The right end of the connecting rod 5562 is movably connected to the U-shaped frame 551 through a bearing. Cam blocks 5563 are fixedly connected to the left and right sides of the outer surface of the connecting rod 5562. The two cam blocks 5563 are distributed in a mirror image. The two cam blocks 5563 do not contact the inner wall of the U-shaped frame 551. The two cam blocks 5563 are located below the placement frame 553.

[0030] With the above solution: the U-shaped frame 551 is fixed to the fixing rod 53 by the fixing block 557, the servo motor 5561 drives the connecting rod 5562 to rotate, the cam block 5563 rotates accordingly and lifts the placement frame 553, the spring 552 cooperates to make it vibrate up and down, the upper culture dish 555 in the placement slot 554 vibrates synchronously with the culture dish assembly 54, so there is no need for multiple culture bottles, batch culture is achieved by the upper culture dish 555 and the culture dish assembly 54, the vibration automation reduces manual operation and reduces errors, the handle block 542 makes it easy to pick up and put down the culture box 541, and improves the operation efficiency and standardization.

[0031] It should be noted that this utility model is a stem cell expansion and culture device. In use, firstly, the viewing door 2 is opened, and the culture dish assembly 54 containing stem cells is placed into the bottom frame 52. The upper culture dish 555 is inserted into the placement slot 554. Parameters are set through the operation key 4, the heating incubator 1 provides the basic temperature, the display screen 3 shows the operating status, and in the uniform heating device 5, the driver 51 drives the bottom frame 52 to rotate, so that the culture dish assembly 54 is heated evenly. In the vibrating and shaking assembly 55, the servo motor 5561 drives the connecting rod 5562 to rotate, the cam block 5563 lifts the placement frame 553, and the spring 552 cooperates to make it vibrate up and down, causing the upper culture dish 555 to shake. The fixing rod 53 firmly supports the vibrating and shaking assembly 55. Therefore, this device simulates a suitable environment for stem cell growth through the cooperation of multiple components, improving the expansion efficiency and quality, and making the operation convenient and controllable.

[0032] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A stem cell expansion culture device comprising a heatable incubator (1), characterized in that: A viewing door (2) is movably installed on the left front end of the heatable incubator (1). A display screen (3) and operation keys (4) are arranged sequentially from back to front on the upper end of the heatable incubator (1). A uniform heating device (5) is fixedly connected to the middle of the lower end of the heatable incubator (1). The upper part of the uniform heating device (5) is located inside the heatable incubator (1). The uniform heating device (5) includes a driver (51). The output end of the driver (51) passes through the lower middle part of the heatable incubator (1) and is fixedly connected to a bottom frame (52). A petri dish assembly (54) is inserted into the bottom frame (52). A fixing rod (53) is fixedly connected to the upper left and upper right parts of the bottom frame (52). A vibrating shaking assembly (55) is inserted and fixedly connected to the upper part of the two fixing rods (53).

2. The stem cell expansion culture device of claim 1, wherein: The culture dish assembly (54) includes a culture box (541), and a handle block (542) is fixedly connected to the front and rear of the outer surface of the culture box (541). Several culture dish bodies (543) are placed inside the culture box (541).

3. The stem cell expansion culture device of claim 2, wherein: The diameter area of ​​the culture box (541) is equal to the inner diameter area of ​​the bottom frame (52), and the culture box (541) is located inside the bottom frame (52).

4. The stem cell expansion culture device of claim 1, wherein: The vibrating and shaking assembly (55) includes a U-shaped frame (551), with springs (552) fixedly connected to the four corners of the lower inner wall of the U-shaped frame (551). The ends of the four springs (552) away from the U-shaped frame (551) are fixedly connected to a placement frame (553). A placement groove (554) is provided at the upper end of the placement frame (553). An upper petri dish (555) is inserted into the placement groove (554). Fixed blocks (557) are fixedly connected to the lower left and lower right ends of the U-shaped frame (551). A vibrating component (556) is embedded in the middle of the inner left wall of the U-shaped frame (551).

5. The stem cell expansion culture device of claim 4, wherein: The square frame (551) is fixedly connected to the upper part of the two fixed rods (53) by two fixed inserts (557). The upper petri dish component (555) and the petri dish assembly (54) are the same in size and structure.

6. The stem cell expansion culture device of claim 4, wherein: The vibration component (556) includes a servo motor (5561), which is embedded in the middle of the inner left wall of the U-shaped frame (551). The output end of the servo motor (5561) is fixedly connected to a connecting rod (5562). The right end of the connecting rod (5562) is movably connected to the U-shaped frame (551) through a bearing. Cam blocks (5563) are fixedly connected to the left and right sides of the outer surface of the connecting rod (5562).

7. The stem cell expansion culture device of claim 6, wherein: The two cam blocks (5563) are distributed in a left-right mirror image. The two cam blocks (5563) do not contact the inner wall of the square frame (551). The two cam blocks (5563) are located together below the placement frame (553).

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