Hermetically sealed multi-well cap and multi-chamber sample preparation device

Abstract

The utility model discloses a kind of full-sealing multi-hole cover and multi-chamber sample preparation device, belong to molecular diagnostic technique field.Full-sealing multi-hole cover includes bottom cover, top cover, layer to be punctured and filter filter core, bottom cover can be connected with the sealing of multi-chamber sample preparation box, bottom cover is provided with first passageway and multiple first through-holes, multiple first through-holes are respectively communicated with multiple chambers of multi-chamber sample preparation box;Top cover and bottom cover are mutually buckled and are surrounded to form gas exchange containing cavity, multiple first through-holes are all communicated with gas exchange containing cavity, the second passageway of top cover is coaxial line connection with first passageway, the groove bottom of mounting groove is communicated with gas exchange containing cavity;Layer to be punctured is connected with top cover and closes second passageway or is connected with bottom cover and closes first passageway, push-pull rod can pass through first passageway and second passageway and puncture layer to be punctured;Filter filter core is set in mounting groove.The utility model can reduce the influence of aerosol in air, improve the accuracy of PCR detection.

Description

Technical Field

[0001] This utility model relates to the field of molecular diagnostic technology, and in particular to a fully sealed porous cap and a multi-chamber sample preparation device. Background Technology

[0002] With the continuous innovation of in vitro diagnostic technology and the in-depth exploration of the concept of precision medicine, molecular diagnostic technology, with its superior sensitivity, rapid detection efficiency, and wide applicability, is gradually becoming an indispensable core tool in the field of medical testing. Among them, PCR (Polymerase Chain Reaction) technology, as the cornerstone of molecular diagnostics, simulates the natural replication process of DNA in vivo to achieve exponential amplification of specific DNA fragments in vitro, providing strong technical support for early disease diagnosis, pathogen identification, and genetic variation analysis. However, from sample collection to obtaining accurate and reliable test results, the PCR testing process involves several precise and complex steps, including sample pretreatment, efficient extraction and purification of nucleic acids, and precise configuration and amplification of the PCR system. This series of processes not only requires the coordinated operation of various specialized equipment but is also time-consuming.

[0003] To address the aforementioned challenges, the existing technical solution CN215517453U, a multi-chamber sample preparation box with a rotating flow channel switching valve, is proposed. This multi-chamber sample preparation box simplifies the PCR detection process and improves detection efficiency. Specifically, the multi-chamber sample preparation box consists of two main parts: the box body and the flow channel switching valve. The box body is meticulously designed with multiple independent chambers. Each chamber has an opening at the top (for loading liquid or venting) and a liquid passage at the bottom. These passages serve as channels for liquid flow and are crucial for achieving material exchange between chambers. The flow channel switching valve, through its unique rotation mechanism, can precisely control the connection between the inlet / outlet and the liquid passages in each chamber, thereby achieving directional flow and distribution of the liquid. Furthermore, a push-pull rod with a piston is cleverly incorporated into the flow channel switching valve's cylinder. The reciprocating motion of the push-pull rod allows for easy extraction or injection of liquid from the chambers.

[0004] However, while multi-chamber sample preparation kits demonstrate significant advantages in simplifying procedures and improving detection efficiency, in practical applications, the open openings of these kits allow aerosols from the air to potentially enter the chambers. These aerosols may carry various microorganisms or nucleic acid fragments, and cross-contamination with the samples within the chambers can directly lead to errors in PCR test results, or even serious consequences such as misdiagnosis. Utility Model Content

[0005] The purpose of this invention is to provide a fully sealed multi-porous cover and multi-chamber sample preparation device that can reduce the influence of aerosols in the air and improve the accuracy of PCR detection.

[0006] To achieve the above objectives, the following technical solution is provided:

[0007] A fully sealed, porous cap for multi-chamber sample preparation kits, including:

[0008] The bottom cover is capable of being sealed to the multi-chamber sample preparation box. The bottom cover is provided with a first channel and a plurality of first through holes. The first channel extends along a first direction, and the plurality of first through holes are respectively connected to a plurality of chambers of the multi-chamber sample preparation box.

[0009] The top cover is fastened to the bottom cover, and a gas exchange containment cavity is formed between the top cover and the bottom cover. Multiple first through holes are connected to the gas exchange containment cavity. The top cover is provided with a second channel and a mounting groove. The second channel extends along the first direction and is coaxially and sealed to the first channel. The bottom of the mounting groove is connected to the gas exchange containment cavity.

[0010] The layer to be punctured is connected to the top cover and closes the second channel, or the layer to be punctured is connected to the bottom cover and closes the first channel. The push-pull rod of the multi-chamber sample preparation box can pass through the first channel and the second channel and puncture the layer to be punctured.

[0011] The filter element is installed in the mounting slot.

[0012] As an optional fully sealed porous cover, the bottom cover has multiple protruding posts on the side near the top cover, each protruding post having a through-hole. The top cover has a second through-hole corresponding to each protruding post, with the end of each protruding post away from the bottom cover extending into the second through-hole. The fully sealed porous cover further includes:

[0013] A sealing film is provided, which covers the side of the top cover away from the bottom cover and is fixedly connected to the top cover. The end of the protruding post away from the bottom cover is bonded to the sealing film. A first clearance hole is provided on the sealing film corresponding to the layer to be punctured, and a second clearance hole is provided on the sealing film corresponding to the mounting groove.

[0014] As an alternative to a fully sealed porous cover, the sealing film is bonded to the top cover, and the adhesive force between the sealing film and the top cover is greater than the adhesive force between the sealing film and the protruding post.

[0015] As an alternative to a fully sealed porous cap, the sealing membrane is made of aluminum foil or plastic.

[0016] As an optional fully sealed porous cover, the filter element is made of sintered filter element or meltblown cloth.

[0017] As an optional solution for the fully sealed porous cover, the fully sealed porous cover also includes a connecting rib, one end of which is connected to the bottom cover and the other end of which is connected to the top cover. The bottom cover, the connecting rib, and the top cover are integrally formed structures.

[0018] As an optional fully sealed porous cover, the inner wall of the first channel is provided with a first annular protrusion, and the outer wall of the second channel is provided with a second annular protrusion, wherein the first annular protrusion and the second annular protrusion are sealed and engaged; and / or

[0019] The bottom cover has a first circumferential edge on the side facing the top cover, and the top cover has a second circumferential edge on the side facing the bottom cover. The projection of the second circumferential edge on the bottom cover is located outside the first circumferential edge. The outer wall surface of the first circumferential edge has a third annular protrusion, and the inner wall surface of the second circumferential edge has a fourth annular protrusion. The third annular protrusion and the fourth annular protrusion are sealed and engaged.

[0020] As an optional fully sealed porous cover, the fully sealed porous cover further includes a sealing assembly, the sealing assembly including a first sealing ring, the first sealing ring being clamped at the junction of the first channel and the second channel, and / or

[0021] The bottom cover has a first circumferential edge on the side facing the top cover, and the top cover has a second circumferential edge on the side facing the bottom cover. The projection of the second circumferential edge on the bottom cover is located outside the first circumferential edge. The sealing assembly also includes a second sealing ring, which is sandwiched between the top cover and the first circumferential edge.

[0022] As an alternative to the fully sealed porous cap, the sealing assembly further includes a connecting portion, one end of which is connected to the first sealing ring, and the other end of which is connected to the second sealing ring.

[0023] A multi-chamber sample preparation apparatus, characterized in that it comprises a multi-chamber sample preparation box and a fully sealed porous cover as described in any of the preceding claims, wherein the fully sealed porous cover is disposed at the opening of the multi-chamber sample preparation box.

[0024] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0025] The fully sealed porous cover provided by this utility model interlocks with the top and bottom covers, forming a gas exchange chamber between them. The fully sealed porous cover, in its interlocked state, is installed on a multi-chamber sample preparation box. The mounting groove, gas exchange chamber, first through-hole, and chambers of the multi-chamber sample preparation box are sequentially connected. Since the filter element is installed in the mounting groove, when the multi-chamber sample preparation box is in operation, air enters the gas exchange chamber through the filter element. This not only facilitates pressure balance but also allows the filter element to filter aerosols in the air entering the gas exchange chamber, improving the accuracy of PCR detection. Because the push-pull rod of the multi-chamber sample preparation box can pass through the first and second channels and puncture the puncture layer on the top or bottom cover, operators can identify whether the fully sealed porous cover is in use by looking at the puncture layer, eliminating the risk of cross-contamination caused by the fully sealed porous cover being mistakenly installed on other multi-chamber sample preparation boxes.

[0026] The multi-chamber sample preparation device provided by this invention has a fully sealed porous cover placed at the opening of the multi-chamber sample preparation box, which can reduce the influence of aerosols in the air and improve the accuracy of detection. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments of this utility model will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the content of the embodiments of this utility model and these drawings without creative effort.

[0028] Figure 1 This is a schematic diagram of the fully sealed porous cover in the snap-fit ​​state in Embodiment 1 of this utility model;

[0029] Figure 2 This is an exploded view of the fully sealed porous cover in Embodiment 1 of this utility model;

[0030] Figure 3 This is a schematic diagram of the fully sealed porous cover in the unfolded state in Embodiment 1 of this utility model;

[0031] Figure 4 This is an exploded view of the fully sealed porous cover in Embodiment 2 of this utility model.

[0032] Figure label:

[0033] 1. Bottom cover; 2. Top cover; 3. Connecting rib; 4. Layer to be punctured; 5. Filter element; 6. Sealing membrane; 61. First clearance hole; 62. Second clearance hole; 7. Sealing assembly; 71. First sealing ring; 72. Second sealing ring; 73. Connecting part;

[0034] 11. First channel; 111. First annular protrusion; 12. First through hole; 13. Protruding post; 14. First perimeter; 141. Third annular protrusion;

[0035] 21. Second channel; 211. Second annular protrusion; 22. Mounting groove; 23. Second through hole; 24. Second perimeter; 241. Fourth annular protrusion. Detailed Implementation

[0036] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. The components of the embodiments of this utility model described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0037] In the description of this utility model, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are used only for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

[0038] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0039] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0040] To reduce the impact of airborne aerosols and improve the accuracy of PCR testing, this embodiment provides a fully sealed multi-hole cap, which is described below in conjunction with... Figures 1 to 4 The specific content of this embodiment will be described in detail. It should be noted that the first direction mentioned in this embodiment is... Figure 1 The Z direction in the equation.

[0041] Example 1

[0042] like Figure 1 As shown, the fully sealed porous cover in this embodiment is used for a multi-chamber sample preparation box. The fully sealed porous cover includes a bottom cover 1, a top cover 2, a puncture-proof layer 4, and a filter element 5, with each part tightly fitted together.

[0043] The bottom cover 1 serves as the foundation for connecting the fully sealed porous cover to the multi-chamber sample preparation box. The bottom cover 1 ensures a sealed connection with the multi-chamber sample preparation box, effectively preventing sample leakage and the intrusion of external impurities. The bottom cover 1 is provided with a first channel 11 and multiple first through holes 12. The first channel 11 extends along a first direction, providing a precise passage path for the push-pull rod of the multi-chamber sample preparation box. The multiple first through holes 12 communicate with multiple chambers of the multi-chamber sample preparation box, ensuring orderly flow of gas or liquid between the cover and the box.

[0044] When the top cover 2 and the bottom cover 1 are fastened together, a gas exchange chamber is formed between them. Multiple first through holes 12 communicate with this gas exchange chamber, allowing gas within the chamber to enter through the first through holes 12, thus laying the foundation for subsequent gas exchange and filtration. The top cover 2 is provided with a second channel 21 and a mounting groove 22. The second channel 21 extends along a first direction and is coaxially and sealed with the first channel 11. This coaxial sealing design ensures tight connection between the channels and smooth gas flow, preventing gas leakage and pressure loss. The bottom of the mounting groove 22 communicates with the gas exchange chamber, providing a reasonable spatial layout for the installation of the filter element 5. The layer to be punctured 4 is connected to the top cover 2 and closes the second channel 21, or the layer to be punctured 4 is connected to the bottom cover 1 and closes the first channel 11. The push-pull rod of the multi-chamber sample preparation box can pass through the first channel 11 and the second channel 21 and puncture the layer to be punctured 4. In this embodiment, the layer to be punctured 4 can be integrally formed with the cover, or it can be separate from the cover. They can be connected by adhesive bonding or heat fusion, and no further restrictions are placed here. The filter element 5 is placed within the mounting groove 22, allowing it to fully perform its filtration function.

[0045] During the operation of a multi-chamber sample preparation box, the pressure in each chamber may change due to factors such as sample addition and reaction progress. The fully sealed porous cover provided by this invention offers an effective solution for maintaining pressure balance through its unique design. The gas exchange containment chamber, as the core area for gas exchange, is connected to each chamber of the multi-chamber sample preparation box through the first through-hole 12, allowing gas within the chambers to freely enter the containment chamber. Simultaneously, outside air enters the gas exchange containment chamber after being filtered by the filter element 5, providing a stable gas supply to the chambers. This gas exchange mechanism can promptly regulate the pressure within the chambers, avoiding adverse effects on the sample preparation process due to excessive or insufficient pressure, thus ensuring the smooth progress of sample preparation.

[0046] Cross-contamination is a significant issue in multi-chamber sample preparation, leading to inaccurate test results and even experimental failure. In this embodiment, the fully sealed porous cap, through the design of the puncture-proof layer 4, provides strong protection against cross-contamination. When unpunctured, the puncture-proof layer 4 completely seals the second channel 21 or the first channel 11. Once the push-pull rod of the multi-chamber sample preparation box punctures the puncture-proof layer 4, the operator can clearly identify that the fully sealed porous cap has been used, thus preventing it from being mistakenly installed on other unused multi-chamber sample preparation boxes. This simple and effective identification method cuts off the transmission path of cross-contamination at its source, greatly improving the safety and reliability of sample preparation.

[0047] PCR, as a highly sensitive molecular biology detection technique, places extremely high demands on the sample preparation environment. Even minute impurities or contaminants can affect the accuracy of the results. The filter element 5 in the fully sealed porous cap efficiently filters aerosols from the air, providing a clean and uncontaminated gaseous environment for sample preparation. During sample preparation, clean air passes through filter element 5 into the gas exchange chamber and then into each chamber of the multi-chamber sample preparation kit, ensuring effective isolation of the sample from external contaminants. This clean gaseous environment minimizes the impact of external factors on the sample, thereby improving the accuracy and reliability of PCR detection and providing more accurate data support for scientific research and clinical diagnosis.

[0048] Furthermore, the bottom cover 1 is provided with a plurality of protruding posts 13 on the side near the top cover 2, and each protruding post 13 is provided with a first through hole 12. The top cover 2 is provided with a second through hole 23 corresponding to the protruding post 13. When the top cover 2 and the bottom cover 1 are fastened together, the end of the protruding post 13 away from the bottom cover 1 extends into the second through hole 23. The fully sealed multi-hole cover also includes a sealing film 6. The sealing film 6 covers the side of the top cover 2 away from the bottom cover 1 and is fixedly connected to the top cover 2. The end of the protruding post 13 away from the bottom cover 1 is bonded to the sealing film 6.

[0049] In multi-chamber sample preparation, the sealed preservation of reagents is crucial. The added sealing membrane 6 can simultaneously seal the first through-holes 12 on multiple protruding pillars 13. This integrated sealing method is more efficient and reliable than the traditional single sealing method, greatly improving the overall sealing performance of the fully sealed multi-hole cover. Whether in static storage or dynamic transportation of the multi-chamber sample preparation box, the sealing membrane 6 maintains a sealed state on the first through-holes 12, providing a safe and stable storage environment for reagents and ensuring sample quality and detection accuracy. When testing is required, the operator only needs to open the top cover 2 on the bottom cover 1. During the opening of the top cover 2, the sealing membrane 6 separates from multiple protruding pillars 13 simultaneously, avoiding the tedious operation of dealing with each sealing point individually in the traditional method, greatly improving the efficiency of membrane removal. The operator does not need to spend a lot of time and effort on removing the sealing membrane 6, and can enter the testing process more quickly. Furthermore, after the top cover 2 is reattached, the precise fit between the protruding post 13 and the second through hole 23, along with the well-designed separation of the sealing membrane 6, quickly opens the first through hole 12 on the protruding post 13, allowing it to connect with the outside environment. Gas can then pass through the filter element 5 into the gas exchange chamber, and then through the first through hole 12 into each chamber of the multi-chamber sample preparation box, achieving pressure balance and gas exchange. This convenient and efficient operating experience not only improves the detection speed but also reduces human error during operation, providing a strong guarantee for the accuracy of the detection results.

[0050] Furthermore, the sealing membrane 6 incorporates a targeted design. A first clearance hole 61 is provided on the sealing membrane 6 corresponding to the layer 4 to be punctured, and a second clearance hole 62 is provided on the sealing membrane 6 corresponding to the mounting groove 22. The first clearance hole 61 provides operational space for the subsequent push-pull rod to puncture the layer 4; the second clearance hole 62 ensures that the filter element 5 can be installed and function properly, without affecting the gas exchange chamber's gas flow and filtration function with the outside world. This meticulous design reflects a comprehensive consideration and optimized integration of all functional modules of the fully sealed porous cover.

[0051] For example, the sealing film 6 is bonded to the top cover 2, wherein the adhesive force between the sealing film 6 and the top cover 2 is greater than the adhesive force between the sealing film 6 and the protruding post 13. This ensures that when the top cover 2 is opened, only the sealing film 6 is allowed to separate from the protruding post 13, preventing the sealing film 6 from falling off the top cover 2. The strong adhesive force between the sealing film 6 and the top cover 2 ensures that the sealing film 6 can adhere tightly to the top cover 2, forming a complete sealing barrier. Even under the influence of external environmental factors such as vibration, impact, or temperature changes, the sealing film 6 will not easily separate from the top cover 2, thus effectively preventing the evaporation of reagents inside the box and the entry of impurities such as air, moisture, and dust. This reliable sealing performance provides a solid foundation for sample quality and detection accuracy, avoiding experimental failures and inaccurate detection results caused by reagent leakage or contamination. Opening the top cover 2 is an essential step when sample testing or gas exchange operations are required. Because the adhesion between the sealing film 6 and the raised post 13 is relatively weak, the sealing film 6 will easily separate from the raised post 13 during the opening of the top cover 2 without detaching from it. This design allows operators to quickly and easily open the top cover 2 without spending extra time and effort dealing with a detached sealing film 6. It also avoids secondary contamination and operational interruptions that could result from the detachment of the sealing film 6, improving operational efficiency and smoothness. Operators can then focus more time and effort on the core aspects of sample preparation and testing, thereby improving the progress and quality of the entire testing process.

[0052] Optionally, the sealing membrane 6 can be made of aluminum foil or plastic. These two materials each have their own characteristics and offer unique advantages in different application scenarios. Aluminum foil has extremely low air and moisture permeability, effectively blocking the penetration of air, moisture, and other gases. For example, in the preparation of some oxygen-sensitive biological reagents, the aluminum foil sealing membrane can prevent oxygen from entering the chamber, avoiding reagent oxidation and thus ensuring reagent quality and detection accuracy. Many reagents are sensitive to light, especially ultraviolet light. Prolonged exposure to light can cause photochemical reactions in reagents, altering their chemical properties and biological activity. Aluminum foil has excellent light-shielding properties, almost completely blocking light penetration. When aluminum foil is used as the sealing membrane 6, it effectively protects the reagents in the multi-chamber sample preparation box from light, ensuring that the reagents are always in a light-protected state during storage and transportation, extending their shelf life and improving experimental reliability. Simultaneously, aluminum foil has good corrosion resistance to most chemical reagents and is not easily corroded by acids, alkalis, or other substances. This allows the aluminum foil sealing membrane to maintain excellent sealing and protection in complex operating environments, ensuring the normal operation of the multi-chamber sample preparation box. Compared to aluminum foil, plastic materials are generally less expensive, which reduces the production cost of fully sealed perforated caps to some extent. Plastic processing is relatively simple, and it can be mass-produced using various methods such as injection molding, blow molding, and hot pressing, resulting in high production efficiency.

[0053] Optionally, the filter element 5 can be made of sintered filter element or meltblown fabric. Sintered filter elements are made from raw materials such as metal powder or polymer powder through a high-temperature sintering process. They possess a large number of uniformly distributed microporous structures, achieving high-precision filtration. During multi-chamber sample preparation, airborne particles, bacteria, viruses, and other contaminants may contaminate reagents and samples, affecting the accuracy of experimental results. Sintered filter elements effectively intercept these micro-contaminants, providing a clean gas environment within the chamber. Furthermore, the stable microporous structure of sintered filter elements prevents deformation and clogging, maintaining stable filtration performance over extended periods, reducing the frequency of filter element replacement and lowering operating costs. Meltblown fabric is an ultra-fine electrostatic fiber cloth made primarily of polypropylene using a meltblown process. Its small fiber diameter, large surface area, and electrostatic properties allow it to efficiently capture airborne particles through both electrostatic adsorption and mechanical interception. Meltblown fabric has a rich pore structure and good air permeability, providing low airflow resistance while ensuring high-efficiency filtration. Both sintered filter elements and meltblown fabric filter elements have their own advantages, which can meet the diverse filtration needs of different users.

[0054] Furthermore, the fully sealed porous cover also includes a connecting rib 3. One end of the connecting rib 3 is connected to the bottom cover 1, and the other end is connected to the top cover 2. The bottom cover 1, connecting rib 3, and top cover 2 are integrally molded structures. The integral molding process combines the manufacturing processes of multiple components into a single unit, reducing assembly steps and production processes. The bottom cover 1 and top cover 2 are connected by the connecting rib 3, achieving an anti-loss function. During the use of the multi-chamber sample preparation box, if the top cover 2 is accidentally placed or improperly operated after opening, it can easily be lost. In this embodiment, the connecting rib 3 connects the top cover 2 to the bottom cover 1, ensuring that the top cover 2 will not completely separate from the bottom cover 1 regardless of whether it is open or closed. This effectively avoids the problem of the top cover 2 being lost, providing convenience for experimental operators and reducing experimental interruptions and increased costs caused by the loss of the top cover 2.

[0055] Furthermore, the inner wall of the first channel 11 is provided with a first annular protrusion 111, and the outer wall of the second channel 21 is provided with a second annular protrusion 211. The first annular protrusion 111 and the second annular protrusion 211 are sealed and engaged; and / or the bottom cover 1 is provided with a first perimeter 14 on the side facing the top cover 2, and the top cover 2 is provided with a second perimeter 24 on the side facing the bottom cover 1. The projection of the second perimeter 24 on the bottom cover 1 is located outside the first perimeter 14. The outer wall of the first perimeter 14 is provided with a third annular protrusion 141, and the inner wall of the second perimeter 24 is provided with a fourth annular protrusion 241. The third annular protrusion 141 and the fourth annular protrusion 241 are sealed and engaged. When the top cover 2 and the bottom cover 1 are fastened together, the engagement of the first annular protrusion 111 with the second annular protrusion 211, and the engagement of the third annular protrusion 141 with the fourth annular protrusion 241, helps to ensure the airtightness of the gas exchange chamber. This prevents reagents inside the multi-chamber sample preparation box from contaminating the external environment, and also prevents contaminants from the external environment from entering the multi-chamber sample preparation box.

[0056] This embodiment also provides a multi-chamber sample preparation device, which includes a multi-chamber sample preparation box and the aforementioned fully sealed porous cover. The fully sealed porous cover is located at the opening of the multi-chamber sample preparation box, which can reduce the influence of aerosols in the air and improve the accuracy of detection. The fully sealed porous cover can seal the multi-chamber sample preparation box and prevent harmful components inside the multi-chamber sample preparation box from volatilizing into the air. Pressure balance is achieved through the filter element 5, and the puncture layer 4 serves as an indicator of obvious changes after use.

[0057] Example 2

[0058] This embodiment provides a fully sealed porous cover. Compared with Embodiment 1, the basic structure of the fully sealed porous cover provided in this embodiment is the same as that in Embodiment 1. The only difference is the sealing method between the top cover 2 and the bottom cover 1. This embodiment will not describe the structure that is the same as that in Embodiment 1 again.

[0059] Furthermore, such as Figure 4 Combination Figure 3 As shown, the fully sealed porous cover also includes a sealing assembly 7. The sealing assembly 7 includes a first sealing ring 71, which is clamped at the joint between the first channel 11 and the second channel 21, and / or a first perimeter 14 is circumferentially provided on the side of the bottom cover 1 facing the top cover 2, and a second perimeter 24 is circumferentially provided on the side of the top cover 2 facing the bottom cover 1. The projection of the second perimeter 24 on the bottom cover 1 is located outside the first perimeter 14. The sealing assembly 7 also includes a second sealing ring 72, which is clamped between the top cover 2 and the first perimeter 14. When the top cover 2 and the bottom cover 1 are fastened together, the sealing assembly 7 ensures the sealing performance at the joint between the first channel 11 and the second channel 21, as well as the sealing performance between the first perimeter 14 and the top cover 2.

[0060] Furthermore, the sealing assembly 7 also includes a connecting portion 73, one end of which is connected to the first sealing ring 71, and the other end of which is connected to the second sealing ring 72. By adding the connecting portion 73, the first sealing ring 71 and the second sealing ring 72 can be formed into a single component, facilitating simultaneous installation and improving installation efficiency.

[0061] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments. Many other equivalent embodiments may be included without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A fully sealed porous cap for use in a multi-chamber sample preparation box, characterized in that, include: Bottom cover (1), the bottom cover (1) can be sealed to the multi-chamber sample preparation box, the bottom cover (1) is provided with a first channel (11) and a plurality of first through holes (12), the first channel (11) extends along a first direction, and the plurality of first through holes (12) are respectively connected to a plurality of chambers of the multi-chamber sample preparation box; The top cover (2) is fastened to the bottom cover (1), and a gas exchange containment cavity is formed between the top cover (2) and the bottom cover (1). A plurality of first through holes (12) are connected to the gas exchange containment cavity. The top cover (2) is provided with a second channel (21) and a mounting groove (22). The second channel (21) extends along the first direction and is coaxially and sealed to the first channel (11). The bottom of the mounting groove (22) is connected to the gas exchange containment cavity. The layer to be pierced (4) is connected to the top cover (2) and closes the second channel (21) or the layer to be pierced (4) is connected to the bottom cover (1) and closes the first channel (11). The push-pull rod of the multi-chamber sample preparation box can pass through the first channel (11) and the second channel (21) and pierce the layer to be pierced (4). The filter element (5) is disposed in the mounting groove (22).

2. The fully sealed porous cap according to claim 1, characterized in that, The bottom cover (1) has a plurality of protruding posts (13) on one side near the top cover (2), each of the protruding posts (13) having a through hole (12). The top cover (2) has a second through hole (23) corresponding to the protruding posts (13), and one end of the protruding post (13) away from the bottom cover (1) extends into the second through hole (23). The fully sealed multi-hole cover also includes: A sealing film (6) is provided, which covers the top cover (2) on the side away from the bottom cover (1) and is fixedly connected to the top cover (2). The end of the protruding post (13) away from the bottom cover (1) is bonded to the sealing film (6). A first clearance hole (61) is provided on the sealing film (6) corresponding to the layer to be punctured (4). A second clearance hole (62) is provided on the sealing film (6) corresponding to the mounting groove (22).

3. The fully sealed porous cap according to claim 2, characterized in that, The sealing film (6) is bonded to the top cover (2), and the adhesive force between the sealing film (6) and the top cover (2) is greater than the adhesive force between the sealing film (6) and the protruding post (13).

4. The fully sealed porous cap according to claim 2, characterized in that, The sealing film (6) is made of aluminum foil or plastic.

5. The fully sealed porous cap according to claim 2, characterized in that, The filter element (5) is made of sintered filter element or meltblown cloth.

6. The fully sealed porous cap according to claim 2, characterized in that, The fully sealed porous cover also includes a connecting rib (3), one end of which is connected to the bottom cover (1), and the other end of which is connected to the top cover (2). The bottom cover (1), the connecting rib (3), and the top cover (2) are an integrally formed structure.

7. The fully sealed porous cap according to any one of claims 2-6, characterized in that, The inner wall of the first channel (11) is provided with a first annular protrusion (111), and the outer wall of the second channel (21) is provided with a second annular protrusion (211). The first annular protrusion (111) and the second annular protrusion (211) are sealed and engaged; and / or The bottom cover (1) has a first perimeter (14) circumferentially arranged on the side facing the top cover (2), and the top cover (2) has a second perimeter (24) circumferentially arranged on the side facing the bottom cover (1). The projection of the second perimeter (24) on the bottom cover (1) is located outside the first perimeter (14). The outer wall surface of the first perimeter (14) is provided with a third annular protrusion (141), and the inner wall surface of the second perimeter (24) is provided with a fourth annular protrusion (241). The third annular protrusion (141) and the fourth annular protrusion (241) are sealed and engaged.

8. The fully sealed porous cap according to any one of claims 2-6, characterized in that, The fully sealed porous cover further includes a sealing assembly (7), which includes a first sealing ring (71) sandwiched at the junction of the first channel (11) and the second channel (21), and / or The bottom cover (1) has a first perimeter (14) circumferentially arranged on the side facing the top cover (2), and the top cover (2) has a second perimeter (24) circumferentially arranged on the side facing the bottom cover (1). The projection of the second perimeter (24) on the bottom cover (1) is located outside the first perimeter (14). The sealing assembly (7) also includes a second sealing ring (72), which is sandwiched between the top cover (2) and the first perimeter (14).

9. The fully sealed porous cap according to claim 8, characterized in that, The sealing assembly (7) further includes a connecting part (73), one end of which is connected to the first sealing ring (71), and the other end of which is connected to the second sealing ring (72).

10. A multi-chamber sample preparation device, characterized in that, The sample preparation box includes a multi-chamber sample preparation box and a fully sealed porous cover as described in any one of claims 1-9, wherein the fully sealed porous cover is disposed at the opening of the multi-chamber sample preparation box.

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