Sample preparation cartridge with preparation fluid containment cavities

Abstract

A sample preparation box with a preparation liquid containing cavity comprises a sampling spoon, a containing part, and a cover part; the sampling spoon comprises a rotating part and a spoon part; the rotating part is fixedly connected with the spoon part; the cover part comprises a rotating connecting part; the rotating part is connected with the rotating connecting part; the containing part comprises an A cavity; in a closed state, the cover part covers an opening part of the A cavity, and the spoon part is in the A cavity; the containing part comprises a D cavity; the D cavity is used for placing a preparation liquid; the preparation liquid comprises a reagent, a staining agent, or a diluent; during a sample preparation process, the preparation liquid is taken from the D cavity and added to the A cavity.

Description

Technical Field

[0001] This application belongs to the field of detection technology, and specifically relates to an apparatus for preparing samples containing solid substances such as feces. Background Technology

[0002] Before biological samples can be tested and analyzed, they need to undergo processing procedures corresponding to the specific testing method. Different testing methods have different requirements and procedures for sample pretreatment. Raw samples typically require specialized operations such as dilution, staining, centrifugation, and concentration. These preparation operations usually need to be performed by testing personnel according to standardized procedures.

[0003] In the process of fecal testing, sample preparation is completed using automated instruments, and a sampling preparation kit has emerged that combines sample collection and sample mixing.

[0004] In the preparation of fecal test samples, some tests require crushing and rotating to separate the target formed elements from the sample. Different tests require the addition of different reagents during the preparation process, such as different types of diluents, stains, and diluent stains. Summary of the Invention

[0005] The preparation of fecal test samples involves various processes. Some tests require crushing and rotating the sample to separate the target components. Different tests require different reagents, such as diluents, stains, and diluent-staining agents. Each reagent requires a different reagent kit and sample preparation box. Furthermore, reagent kits, diluent boxes, and preparation boxes are all disposable consumables. The production, transportation, and use of these kits require careful consideration of their compatibility, leading to complex management, high costs, and significant environmental impact due to their disposable nature. This application proposes integrating these disposable consumables—reagent kits, diluent boxes, and preparation boxes—into a single consumable, reducing management complexity and decreasing the quantity and volume of disposable consumables.

[0006] A sample preparation box with a preparation solution containing a cavity includes: a sampling spoon, a receiving part, and a cover; the sampling spoon includes a rotating part and a spoon part; the rotating part and the spoon part are fixedly connected; the cover includes a rotating connecting part; the rotating part and the rotating connecting part are connected; the receiving part includes cavity A; in the closed state, the cover covers the opening of cavity A, and the spoon part is in cavity A; the receiving part includes cavity D, which is used to hold the preparation solution, which includes reagents, staining agents, or diluents; during sample preparation, the preparation solution is taken from cavity D and added to cavity A.

[0007] It is possible that the receiving part also includes cavity B; a filter partition is provided between cavity A and cavity B.

[0008] Alternatively, the receiving part may also include an E-cavity, which is a detection cavity. The sidewalls of the E-cavity are made of transparent material. The E-cavity is used to detect the optical properties or deposition layering properties of the sample.

[0009] It is possible that the sidewalls of cavity A and / or cavity B are made of transparent material, and cavity A and / or cavity B are also used to detect the optical properties or deposition layering properties of the sample.

[0010] It is possible that the receiving part includes a C-cavity, in which an absorbent component is placed.

[0011] Yes, the absorbent component can be wrapped around the filter screen.

[0012] It is possible that a filter baffle B is provided between cavity B and cavity C; the filter baffle B includes a filter window, and the filter window includes a filter screen.

[0013] Yes, the receiving part may also include an F-cavity, which is used to hold the suction gun tip.

[0014] Yes, it can also include a sealing membrane that covers cavity D.

[0015] Yes, it can also include a sealing membrane that covers cavity E.

[0016] One of the technical benefits of the above-mentioned technical solution is that it integrates disposable consumables such as reagent kits, diluent boxes, and preparation boxes, which have complete configuration requirements, into one consumable, thereby reducing management complexity.

[0017] One of the technical benefits of the above-mentioned technical solution is that integrating the reagent kit and preparation box into one device facilitates the operation of automated equipment and reduces the cost of automated equipment.

[0018] One of the technical benefits of the above-mentioned technical solution is that it integrates the reagent kit and preparation box into one device, reducing the quantity and volume of consumables.

[0019] One of the technical benefits of the above-mentioned solution is that integrating the reagent kit and preparation box into one device can improve the efficiency of reagent retrieval during sample preparation.

[0020] One of the technical effects of the above-mentioned technical solution is that the detection chamber can improve the efficiency of detection after sample preparation.

[0021] One of the technical benefits of the above-mentioned solution is that it reduces costs by protecting the reagent kit or detection chamber with a thin film.

[0022] One of the technical advantages of the above-mentioned technical solution is that by protecting the reagent kit or detection chamber with a thin film, the pipette tip can be used to directly puncture the thin film during the detection process, which facilitates the operation of automated equipment.

[0023] One of the technical advantages of the above-mentioned technical solution is that cavity A can also be used to detect the optical properties or deposition layering properties of the sample, which facilitates detection. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of a three-dimensional view of Embodiment 1;

[0025] Figure 2 This is a schematic diagram of a partial exploded view of Embodiment 1;

[0026] Figure 3 This is a schematic diagram of the line section view in Example 1;

[0027] Figure 4 This is a schematic diagram of a three-dimensional view of Embodiment 2;

[0028] Figure 5 This is a schematic diagram of a partial exploded view of Embodiment 2;

[0029] Figure 6 This is a schematic diagram of a three-dimensional view of Embodiment 3. Detailed Implementation

[0030] The content of this application will be further described in detail below with reference to the accompanying drawings. It should be noted that the following description is of preferred embodiments of this application and does not constitute any limitation on this application. The description of the preferred embodiments is merely an explanation of the general principles of this application. The use of terms such as "first," "second," and "A," "B" in this application is for ease of explanation only and does not represent a temporal or spatial order. The combinations of letters and numbers "S," "M," and "H" in this application are also for ease of explanation, and their specific meanings are determined by the specific content they refer to.

[0031] Figure 1 This is a schematic diagram of a three-dimensional view of Embodiment 1.

[0032] Figure 2 This is a schematic diagram of a partial exploded view of Embodiment 1.

[0033] A sample preparation box with a preparation liquid containing cavity includes: a sampling spoon 0210, a containing part 0110, a cover part 0120, and a D-cavity 0150; the sampling spoon includes a rotating part 0211 and a spoon part 0212; the rotating part is fixedly connected to the spoon part; the cover part includes a rotating connecting part 0130; the rotating part is connected to the rotating connecting part.

[0034] Figure 3 This is a schematic diagram of the line section view in Example 1.

[0035] The receiving part includes cavity A 0310; in the closed state, the cover covers the opening of cavity A, and the spoon is in cavity A; the receiving part includes cavity D 0320, cavity D is used to hold the preparation solution, which includes reagents, staining agents or diluents; during sample preparation, the preparation solution is taken from cavity D and added to cavity A.

[0036] It can be added to cavity A in advance, or it can be added to cavity A through the liquid addition port 0330 during the preparation process.

[0037] The receiving part also includes cavity B; a filter partition 0160 is provided between cavity A and cavity B 0350.

[0038] The receiving part includes a C-cavity 0360, in which a water-absorbing component is placed.

[0039] The water-absorbing component is wrapped around the filter screen.

[0040] The housing also includes an E-cavity 0370, which is a detection cavity. The sidewalls of the E-cavity are made of transparent material. The E-cavity is used to detect the optical properties or deposition layering properties of the sample.

[0041] The sidewalls of cavity A and / or cavity B are made of transparent material. Cavity A and / or cavity B are also used to detect the optical properties or deposition layering properties of the sample.

[0042] A filter baffle 0352 is provided between cavity B and cavity C; the filter baffle includes a filter window, and the filter window includes a filter screen.

[0043] Figure 4 This is a schematic diagram of a three-dimensional view of Embodiment 2.

[0044] Figure 5 This is a schematic diagram of a partial exploded view of Embodiment 2.

[0045] The receiving part includes cavity F 0410, which is used to hold the suction gun tip 0411.

[0046] like Figure 3 It also includes a sealing membrane that covers cavity D. Figure 3 The sealing membrane is not shown in the drawing.

[0047] like Figure 3 It also includes a sealing membrane that covers cavity E. Figure 3 The sealing membrane is not shown in the drawing.

[0048] Figure 6 This is a schematic diagram of a three-dimensional view of Embodiment 3.

[0049] Figure 6In this embodiment, the arrangement of cavity D 0610, cavity E 0620, and cavity F 0630 differs from that in embodiment 1 or embodiment 2. Cavity E 0620 is narrower and is placed on the outermost side.

[0050] While this application has been described and illustrated with reference to preferred embodiments and several alternatives, it is not intended to be limited to the specific descriptions herein. Other alternatives or equivalent components may also be used to practice this application.

Claims

1. A sample preparation box with a preparation liquid containing cavity, characterized in that, include: Sampling spoon, receiving part, lid; The sampling spoon includes a rotating part and a spoon part; the rotating part and the spoon part are fixedly connected. The cover includes a rotatable connecting part; the rotatable part is connected to the rotatable connecting part; The receiving portion includes cavity A; The receiving portion includes a D-cavity; In the closed state, the cover covers the opening of cavity A, and the spoon is inside cavity A; The D chamber is used to hold the preparation solution, which may include reagents, staining agents, or diluents. During sample preparation, the preparation solution is taken from cavity D and added to cavity A.

2. The sample preparation box with a preparation liquid containing cavity according to claim 1, characterized in that, The receiving section also includes cavity B; a filter partition is provided between cavity A and cavity B.

3. The sample preparation box with a preparation liquid containing cavity according to claim 1, characterized in that, The housing also includes an E-cavity, which is a detection cavity. The sidewalls of the E-cavity are made of transparent material. The E-cavity is used to detect the optical properties or deposition layering properties of the sample.

4. The sample preparation box with a preparation liquid containing cavity according to claim 2, characterized in that, The sidewalls of cavity A and / or cavity B are made of transparent material. Cavity A and / or cavity B are also used to detect the optical properties or deposition layering properties of the sample.

5. The sample preparation box with a preparation liquid containing cavity according to claim 1, characterized in that, The receiving part includes a C-cavity, in which a water-absorbing component is placed.

6. The sample preparation box with a preparation liquid containing cavity according to claim 5, characterized in that, The water-absorbing component encloses the filter screen.

7. The sample preparation box with a preparation liquid containing cavity according to claim 5, characterized in that, A filter baffle B is installed between cavity B and cavity C; B. The filter baffle includes a filter window, and the filter window includes a filter screen.

8. The sample preparation box with a preparation liquid containing cavity according to any one of claims 1 to 7, characterized in that, The receiving section also includes an F-cavity, which is used to hold the suction gun tip.

9. The sample preparation box with a preparation liquid containing cavity according to claim 1, characterized in that, It also includes a sealing membrane that covers the D cavity.

10. The sample preparation box with a preparation liquid containing cavity according to claim 3, characterized in that, It also includes a sealing membrane that covers the E cavity.

Images