A rat inhalation exposure test apparatus

Abstract

The utility model discloses a rat inhale exposure toxicant test equipment belongs to the field of biological toxicant device. Rat inhale exposure toxicant test equipment includes toxicant gas generating device and rat toxicant cabin device. Toxicant gas generating device includes: shell, gas generating cabin, sealed door, heating part, humidification subassembly, first temperature detection spare and first humidity detection spare, and the detection end is in the gas generating cabin inner chamber. Rat toxicant cabin device includes: toxicant cabin, cabin door, the rat cage of being arranged in the experiment cavity of toxicant cabin, second temperature detection spare and second humidity detection spare, and the detection end is in the experiment cavity of toxicant cabin. The gas inlet line and the return gas pipeline are connected with the gas generating cabin and the toxicant cabin respectively. The gas generating cabin and the toxicant cabin adopt independent structure, and through the gas circulation pipeline, the concentration of toxicant gas in the toxicant cabin can be kept uniform during the whole toxicant process, which provides reliable equipment support for scientific research conclusion.

Description

Technical Field

[0001] This utility model relates to the field of biological exposure devices, and in particular to a rat inhalation exposure testing device. Background Technology

[0002] In toxicology and environmental health research, inhalation exposure testing is a crucial experimental method for assessing the toxic effects of chemicals, air pollutants, or volatile compounds on organisms (such as rats). Traditional gas exposure systems typically employ open or semi-closed designs, which suffer from problems such as unstable gas concentrations, inaccurate temperature and humidity control, gas waste, and environmental pollution. Furthermore, existing systems often lack closed-loop control of the gas generation chamber and the exposure chamber, making it difficult to maintain consistent experimental conditions and affecting the reliability and repeatability of experimental data.

[0003] Currently, although some toxic testing equipment has temperature and humidity control functions, it usually uses a single sensor for monitoring, which cannot compare and adjust the environmental parameters of the gas generating chamber and the toxic chamber in real time, which may lead to a deviation between the environment in which the experimental animals are located and the actual state of the toxic gas. Utility Model Content

[0004] This invention provides a rat inhalation exposure testing device. The system is a closed-loop testing device that can accurately control the temperature and humidity of the toxic gas and achieve dynamic balance between the gas generation chamber and the toxic chamber, thereby improving the controllability and repeatability of the experiment.

[0005] A rat inhalation exposure test device includes a toxic gas generator and a rat exposure chamber device connected in a closed loop via an inlet pipe and a return pipe.

[0006] The contaminated gas generating device includes:

[0007] - The outer casing has an inner cavity and an opening communicating with the inner cavity;

[0008] - A gas generating chamber is located inside the outer shell cavity, with its chamber opening facing the same direction as the outer shell opening; the test plate is placed inside the gas generating chamber cavity;

[0009] - A sealed door, located at the opening end of the outer shell, is used to open and close the inner cavity of the gas generating chamber;

[0010] - Heating element, located inside the gas generating chamber, used to heat the inside of the gas generating chamber;

[0011] - Humidification component, used to spray humidifying water into the gas generating chamber cavity;

[0012] - The first temperature detector and the first humidity detector are respectively installed on the side wall of the gas generating chamber, and the detection end is in the inner cavity of the gas generating chamber;

[0013] The rat poisoning chamber device includes:

[0014] - The poison chamber is equipped with an experimental chamber and a chamber opening that connects to the experimental chamber;

[0015] - The hatch, located at the opening end of the contaminated chamber, is used to open and close the experimental chamber;

[0016] -Rat cages, located inside the experimental chamber of the poison treatment chamber;

[0017] - The second temperature detector and the second humidity detector are respectively installed on the side wall of the exposure chamber, and the detection end is in the experimental chamber of the exposure chamber;

[0018] - An oxygen detection sensor is installed in the experimental chamber of the poison treatment chamber;

[0019] The intake and return air lines are respectively connected to the gas generation chamber and the contamination chamber.

[0020] According to the aforementioned rat inhalation exposure test equipment, a clean gas inlet pipe is provided on the side wall of the gas generating chamber. One end of the clean gas inlet pipe communicates with the inner cavity of the gas generating chamber, and the other end extends out of the outer shell and communicates with the outside. An inlet control valve is provided on the clean gas inlet pipe.

[0021] The side wall of the exposure chamber is equipped with an exhaust pipe. One end of the exhaust pipe is connected to the experimental chamber of the exposure chamber, and the other end extends out of the exposure chamber and connects to the outside. An exhaust control valve is installed on the exhaust pipe.

[0022] Furthermore, a first gas sampling pipe is provided on the side wall of the gas generating chamber. One end of the first gas sampling pipe communicates with the inner cavity of the gas generating chamber, and the other end extends out of the outer shell and communicates with the outside. A first gas sampling control valve is provided on the first gas sampling pipe.

[0023] A second gas sampling pipe is installed on the side wall of the exposure chamber. One end of the second gas sampling pipe is connected to the experimental chamber of the exposure chamber, and the other end extends out of the exposure chamber and connects to the outside. A second gas sampling control valve is installed on the second gas sampling pipe.

[0024] According to the aforementioned rat inhalation exposure test equipment, an airflow control device is installed on the air intake pipe, which includes a fan, a wind speed sensor and a gas cooling device.

[0025] The gas cooling device includes a heat sink mounted on the air intake pipe and a cooling fan attached to the heat sink.

[0026] According to the aforementioned rat inhalation exposure test equipment, the toxic gas generating device also includes: a centrifugal fan and fins installed in the inner cavity of the gas generating chamber;

[0027] The rat poisoning chamber device also includes at least one stirring fan installed on the inner wall of the poisoning chamber.

[0028] The rat inhalation exposure testing device also includes a controller and a control panel; the controller is located inside the housing, and the control panel is located on the housing.

[0029] The controller is electrically connected to the heating element, humidification component, first temperature sensor, first humidity sensor, second temperature sensor, second humidity sensor, oxygen sensor, and control panel.

[0030] According to the aforementioned rat inhalation exposure test equipment, the rat cage includes a bottom plate and side wall plates, both of which have openings.

[0031] Furthermore, the rat poisoning chamber device also includes:

[0032] - Two first support members are provided, which are fixed to the inner wall of the contaminated chamber and arranged opposite each other; the first support member has an inverted F-shaped structure, including a vertical mounting plate and a first horizontal plate and a second horizontal plate perpendicular to the side of the vertical mounting plate; the vertical mounting plate is fixedly connected to the inner wall of the contaminated chamber; a sliding groove is formed between the first horizontal plate and the second horizontal plate; the first horizontal plate is used to support the rat cage;

[0033] - The sludge collection tray is slidably installed in the chute.

[0034] Furthermore, the rat poisoning chamber device also includes:

[0035] - Functional composite membrane, installed inside the rat cage; the functional composite membrane consists of two layers: a metal mesh substrate and a silicone film microporous coating; the surface of the metal mesh substrate is coated with a conductive antibacterial coating functional composite membrane; the functional composite membrane is connected to the cage grounding rail via a snap-fit ​​connection to form an electrostatic discharge path.

[0036] Furthermore, the metal mesh substrate is a honeycomb mesh woven from 316L medical stainless steel wire or titanium alloy wire, with the mesh hole side length or hole diameter ≤0.3cm;

[0037] The surface micropores of the silicone film microporous coating are treated with a pore size of 5~10μm.

[0038] The rat inhalation exposure testing device provided by this invention has at least the following advantages compared with the prior art:

[0039] (1) The gas generating chamber and the poisoning chamber of the rat inhalation exposure test device of this utility model adopt independent structures. Through the gas circulation pipeline, the concentration of poisoning gas in the poisoning chamber can be kept uniform throughout the poisoning process, which significantly improves the accuracy of the experiment and provides reliable equipment support for scientific research conclusions.

[0040] (2) The temperature, humidity, and airflow control device of the rat inhalation exposure test equipment of this utility model can monitor key parameters such as temperature, humidity, and gas flow rate inside the exposure chamber in real time, ensuring that the experimental environment is always stable. This provides a reliable environmental guarantee for the study of the toxicity of volatile substances in the test plate.

[0041] (3) The rat cage of the rat inhalation exposure test device of this utility model is equipped with a functional composite membrane, which realizes the three functions of "antibacterial-conductive-breathable" in one, and the multi-functional integrated design and the elimination of the root cause of experimental error provide a revolutionary platform for high-precision toxicology research. Attached Figure Description

[0042] Figure 1 Schematic diagram of the three-dimensional structure of the rat inhalation exposure test device. Figure 1 ;

[0043] Figure 2 Schematic diagram of the three-dimensional structure of the rat inhalation exposure test device. Figure 2 ;

[0044] Figure 3 This is a side view of the rat inhalation exposure test equipment.

[0045] Figure 4 A schematic diagram of the internal structure and connection structure of the gas generating chamber;

[0046] Figure 5 for Figure 4 A magnified view of a section at point A in the middle;

[0047] Figure 6 A three-dimensional structural diagram of the gas generating chamber and its internal structure;

[0048] Figure 7 A three-dimensional structural diagram of the poisoning chamber and its internal structure;

[0049] Figure 8 This is a three-dimensional structural diagram of the first support component;

[0050] Figure 9 This is a three-dimensional structural diagram of a mouse cage;

[0051] Figure 10 A three-dimensional structural diagram of the mouse cage and the functional composite membrane;

[0052] Figure 11 A cross-sectional view of the rat cage and the functional composite membrane;

[0053] Figure 12 for Figure 11 A magnified view of a section at point B in the middle;

[0054] Figure 13This is a magnified view of a portion of the functional composite membrane.

[0055] Explanation of reference numerals in the attached figures:

[0056] 100. Intake pipe;

[0057] 200. Return gas pipeline;

[0058] 300. Toxic gas generator; 301. Outer casing; 302. Gas generating chamber; 303. Sealing door; 304. Heating element; 305. Humidification assembly; 306. First temperature sensor; 307. First humidity sensor; 308. Clean gas inlet pipe; 309. Inlet control valve; 310. First gas sampling pipe; 311. First gas sampling control valve; 312. Second support component; 313. Centrifugal fan; 314. Fins; 3051. Water tank; 3052. Water suction pipe; 3053. Water pump; 3054. Water delivery pipe; 3055. Atomizing nozzle; 3056. Liquid level sensor;

[0059] 400. Rat poisoning chamber device; 401. Poisoning chamber; 402. Chamber door; 403. Rat cage; 404. Second temperature detection element; 405. Second humidity detection element; 406. Exhaust pipe; 407. Exhaust control valve; 408. Second gas sampling pipe; 409. Second gas sampling control valve; 410. First support component; 411. Sludge collection tray; 412. Functional composite membrane; 413. Stirring fan; 414. Oxygen detection sensor; 4031. Base plate; 4032. Side wall plate; 4101. Vertical mounting plate; 4102. First horizontal plate; 4103. Second horizontal plate; 4121. Metal mesh substrate; 4122. Silicone film microporous coating;

[0060] 500. Test material;

[0061] 600. Controller;

[0062] 700. Control Panel;

[0063] 800. Airflow control device; 801. Circulating fan; 802. Wind speed sensor; 803. Gas cooling device; 8031. Heat sink; 8032. Cooling fan. Detailed Implementation

[0064] To make the technical problem to be solved, the technical solution and advantages of this utility model clearer, the following will be described in conjunction with the accompanying drawings. Figures 1 to 13 The technical solution of this utility model is clearly and completely described in conjunction with specific embodiments.

[0065] like Figures 1 to 5As shown, the rat inhalation exposure test equipment includes a toxic gas generator 300 and a rat exposure chamber 400 connected in a closed loop via an inlet pipe 100 and a return pipe 200. The toxic gas generator 300 generates polluted gas, and the rat exposure chamber 400 is the enclosure for rat activity. The toxic gas generated by the toxic gas generator 300 enters the rat exposure chamber 400 through the inlet pipe 100, causing the rats to inhale / be exposed to the toxic gas, eliciting physiological responses. The closed loop of the inlet pipe 100 and the return pipe 200 forms a gas circulation system, preventing excessive pressure inside the exposure chamber caused by continuous input of toxic gas, which could affect the normal physiological state of the rats and lead to experimental errors.

[0066] The toxic gas generating device 300 includes a housing 301, a gas generating chamber 302, and a sealing door 303. The housing 301 has an inner cavity and an opening communicating with the inner cavity. The gas generating chamber 302 is located within the inner cavity of the housing 301, with its opening facing the same direction as the outer cavity opening; the test plate 500 is placed within the inner cavity of the gas generating chamber 302. The sealing door 303 is located at the opening end of the housing 301 and is used to open and close the inner cavity of the gas generating chamber 302. A heating element 304 is installed within the inner cavity of the gas generating chamber 302 for heating the inner cavity. The toxic gas generating device 300 also includes a humidification assembly 305 for spraying humidifying water into the inner cavity of the gas generating chamber 302. The toxic gas generating device 300 also includes a first temperature detection element 306 and a first humidity detection element 307, respectively installed on the side wall of the gas generating chamber 302, with their detection ends located within the inner cavity of the gas generating chamber 302.

[0067] The test board 500 can be made of a variety of synthetic or natural materials, including synthetic sports field materials (such as artificial turf, synthetic running tracks, rubber mats, etc.), decorative and finishing materials (such as flooring materials, wall materials, adhesives and sealants, etc.), automotive interior materials, furniture materials, etc. The rat inhalation exposure testing device can study the ability of different materials to release gases under specific temperature and humidity conditions, providing more accurate data support for risk assessment.

[0068] The contaminated gas generating device 300 also includes two second support members 312, which are fixed to the inner wall of the gas generating chamber 302 and arranged opposite to each other. Specifically, the second support member 312 has an L-shaped structure, including a vertical plate and a horizontal plate perpendicular to the side of the vertical plate. The vertical plate is fixedly connected to the inner wall of the gas generating chamber 302, and the horizontal plate is used to support the test plate 500.

[0069] The rat poisoning chamber device 400 includes a poisoning chamber 401 and a door 402. The poisoning chamber 401 has an experimental cavity and a chamber opening communicating with the experimental cavity. The door 402 is located at the opening end of the poisoning chamber 401 and is used to open and close the experimental cavity. A rat cage 403 is provided in the experimental cavity of the poisoning chamber 401. The rat poisoning chamber device 400 also includes a second temperature detector 404 and a second humidity detector 405, respectively disposed on the side wall of the poisoning chamber 401, with the detection ends inside the experimental cavity of the poisoning chamber 401.

[0070] The intake pipe 100 and the return pipe 200 connect the gas generating chamber 302 and the contamination chamber 401, respectively. Specifically, the intake pipe 100 is connected to the gas generating chamber 302 near its top side wall, and the intake pipe 100 is connected to the contamination chamber 401 near its top side wall. The return pipe 200 is connected to the gas generating chamber 302 near its bottom side wall, and the return pipe 200 is connected to the contamination chamber 401 near its bottom side wall. This connection arrangement ensures the uniformity of the gas supply.

[0071] The gas generating chamber 302 and the poisoning chamber 401 adopt independent structures to reduce the interference of high temperature environment on the physiological state of rats, thereby improving the reliability of experimental data and making the experimental results more convincing.

[0072] The exposure chamber 401 is made of transparent material, allowing researchers to clearly observe the behavior and physiological changes of rats during exposure. The specific material for the exposure chamber 401 can be acrylic, polycarbonate, or transparent glass.

[0073] The rat inhalation exposure testing device includes a controller 600 and a control panel 700. The controller 600 is housed inside the outer casing 301, and the control panel 700 is mounted on the casing 301. The controller 600 is electrically connected to the heating element 304, the humidification component 305, the first temperature sensor 306, the first humidity sensor 307, the second temperature sensor 404, the second humidity sensor 405, and the control panel 700. The controller 600 is a PLC controller or a chip-based controller. The control panel 700 integrates a display screen and operation buttons, allowing for the setting of the temperature and humidity inside the gas generation chamber 302. Researchers can conveniently set the concentration of the toxic gas, adjust the flow rate, and view environmental parameters through this control panel, achieving centralized control of the entire experimental process.

[0074] Specifically, the heating element 304 can be a resistance wire, which has a fast response speed, low thermal inertia, and heats up rapidly after being energized. Alternatively, the heating element 304 can be a ceramic heating element, which reduces power consumption after reaching the set temperature, and ceramic heating elements offer high temperature control accuracy (±1℃). The heating element 304 is electrically connected to the controller, which controls the heating process of the heating element 304.

[0075] Specifically, in this embodiment, the first temperature detection element 306 and the second temperature detection element 404 include any one of a temperature sensor, an infrared sensing device, and a temperature and humidity sensor. The first humidity detection element 307 and the second humidity detection element 405 include a humidity sensor or a temperature and humidity sensor. The temperature and humidity measuring devices described above are easy to install and set up, provide accurate and reliable measurement data, and are easy to integrate with the controller for data transmission. The monitored data is transmitted to the control panel 700 in real time. Experimenters can monitor the environmental conditions inside the chamber at any time through the control panel 700. If environmental parameters exceed the set range, timely adjustments can be made through the control system to ensure the stability of the experimental environment.

[0076] The experimental chamber 401 of the poison exposure chamber is also equipped with an oxygen detection sensor 414. The oxygen detection sensor 414 is electrically connected to the controller 600 and can detect the oxygen concentration data in the experimental chamber 401 and send it to the controller 600.

[0077] Furthermore, a clean gas inlet pipe 308 is provided on the side wall of the gas generating chamber 302. One end of the clean gas inlet pipe 308 communicates with the inner cavity of the gas generating chamber 302, and the other end extends out of the outer shell 301 to communicate with the outside. The clean gas inlet pipe 308 is used to connect to a clean gas cylinder to provide clean gas to the interior of the gas generating chamber 302. An inlet control valve 309 is provided on the clean gas inlet pipe 308. An exhaust pipe 406 is provided on the side wall of the contamination chamber 401. One end of the exhaust pipe 406 communicates with the experimental chamber of the contamination chamber 401, and the other end extends out of the contamination chamber 401 to communicate with the outside, used to discharge the gas in the inner cavity of the contamination chamber 401. An exhaust control valve 407 is provided on the exhaust pipe 406. The inlet control valve 309 and the exhaust control valve 407 are manual control valves, and the operator manually operates the inlet and exhaust process. The inlet control valve 309 and the exhaust control valve 407 can also be electric control valves, electrically connected to the controller, for intelligent control of the inlet and exhaust process.

[0078] The rat cage 403 containing the test rat is placed into the experimental chamber of the exposure chamber 401. The chamber door 402 is closed. The test plate 500 is placed into the cavity of the gas generating chamber 302. After closing the sealing door 303, the clean gas inlet pipe 308 is connected to the clean gas cylinder. The inlet control valve 309 and the exhaust control valve 407 are opened and maintained for a period of time until the background gas inside the gas generating chamber 302 and the exposure chamber 401 is exhausted. Then, the testing process begins. This setup can improve the accuracy of the test results.

[0079] Furthermore, a first gas sampling pipe 310 is provided on the side wall of the gas generating chamber 302. One end of the first gas sampling pipe 310 communicates with the inner cavity of the gas generating chamber 302, and the other end extends out of the outer shell 301 to communicate with the outside. A first gas sampling control valve 311 is provided on the first gas sampling pipe 310. A second gas sampling pipe 408 is provided on the side wall of the contamination chamber 401. One end of the second gas sampling pipe 408 communicates with the experimental chamber of the contamination chamber 401, and the other end extends out of the contamination chamber 401 to communicate with the outside. A second gas sampling control valve 409 is provided on the second gas sampling pipe 408. The first gas sampling control valve 311 and the second gas sampling control valve 409 are manual control valves, and the operator manually operates the gas sampling process; the first gas sampling control valve 311 and the second gas sampling control valve 409 can also be electric control valves, electrically connected to the controller, for intelligent control of the gas sampling process.

[0080] The first gas sampling pipe 310 and the second gas sampling pipe 408 can be connected to a gas analyzer to collect gas from the gas generating chamber 302 and the contamination chamber 401 and detect the concentration of pollutants. During the gas sampling process, the inlet control valve 309 can be opened to replenish gas to the gas generating chamber 302 and the contamination chamber 401 to maintain stable gas pressure inside the chambers.

[0081] The humidification component 305 is either an ultrasonic humidifier or a heated humidifier. In this embodiment, the humidification component 305 is a heated humidifier. The heated humidifier includes a water tank 3051, a water suction pipe 3052, a water pump 3053, a water delivery pipe 3054, and an atomizing nozzle 3055. The water tank 3051 is housed inside the outer casing 301, and has an outlet at its bottom. One end of the water suction pipe 3052 is connected to the outlet of the water tank 3051, and the other end is connected to the inlet of the water pump 3053. The outlet of the water pump 3053 is connected to one end of the water delivery pipe 3054, and the other end extends into the inner cavity of the gas generating chamber 302 and connects to the atomizing nozzle 3055. A heater is installed in the water tank 3051 to heat the water in the tank. Furthermore, the humidification assembly 305 also includes a liquid level sensor 3056 disposed in the water tank 3051. The liquid level sensor 3056 is electrically connected to the controller 600 to detect the water level in the water tank 3051. The water pump 3053 is electrically connected to the controller 600 to control the operation and shutdown of the water pump 3053.

[0082] The level sensor 3056 may include a float level sensor, a capacitive level sensor, or a magnetostrictive level sensor.

[0083] According to the aforementioned rat inhalation exposure testing equipment, an airflow control device 800 is installed on the air intake pipe 100. The airflow control device 800 includes a circulating fan 801, a wind speed sensor 802, and a gas cooling device 803. The gas cooling device 803 includes a heat sink 8031 ​​sleeved on the air intake pipe 100 and a cooling fan 8032 attached to the heat sink 8031. The controller 600 is electrically connected to the circulating fan 801, the wind speed sensor 802, and the cooling fan 8032.

[0084] Specifically, the circulating fan 801 can adjust its operating power. The circulating fan 801 and the wind speed sensor 802 work together to adjust the gas flow rate injected into the experimental chamber of the contamination chamber, ensuring that the contaminant gas enters the experimental chamber of the contamination chamber according to the concentration and rate set in the experiment.

[0085] Before the toxic gas is introduced into the toxic chamber 401, it must pass through the gas cooling device 803 to prevent the high-temperature toxic gas from directly entering the experimental chamber of the toxic chamber 401 and affecting the health and activity of the rats.

[0086] The temperature, humidity, and airflow control device of this invention's rat inhalation exposure testing equipment can monitor key parameters such as temperature, humidity, and gas flow rate inside the exposure chamber in real time, ensuring that the experimental environment remains stable. This provides a reliable environmental guarantee for the study of the toxicity of volatile substances in test panels.

[0087] Furthermore, such as Figure 6 As shown, the contaminated gas generating device 300 also includes a centrifugal fan 313 and fins 314 disposed inside the gas generating chamber 302, which can ensure uniform distribution of gas, temperature, and humidity inside the gas generating chamber 302. The centrifugal fan 313 is electrically connected to the controller 600 to control the operation and stop of the centrifugal fan 313.

[0088] The rat poisoning chamber device 400 further includes at least one stirring fan 413 disposed on the inner wall of the poisoning chamber 401, which can make the gas, temperature and humidity in the poisoning chamber 401 uniformly distributed. The stirring fan 413 is electrically connected to a controller to control the operation and stop of the stirring fan 413.

[0089] In some embodiments, the sealing door 303 can be opened or closed by rotation. Optionally, the sealing door 303 is rotatably connected to the opening end of the outer shell 301 via a hinge structure. The sealing door 303 can rotate by flipping or by a double-door mechanism. In some embodiments, the door 2 can be opened or closed by sliding. Optionally, the sealing door 303 is slidably connected to the outer shell 301 via a slide rail structure. It should be noted that a sealing ring is provided between the outer shell 301 and the sealing door 303 to prevent leakage of pollutant gas. The connection method between the opening ends of the hatch 402 and the contaminated chamber 401 is the same as the connection method between the opening ends of the sealing door 303 and the outer shell 301. It is understood that the opening ends of both the outer shell 301 and the gas generating chamber 302 are flush, and there is a connecting sealing plate between the walls of the outer shell 301 and the gas generating chamber 302. When the sealing door 303 is closed, the openings of the outer shell 301 and the gas generating chamber 302 are closed simultaneously.

[0090] like Figure 9 As shown, the rat cage 403 includes a base plate 4031 and a side wall plate 4032. Both the base plate 4031 and the side wall plate 4032 are provided with openings, which allow gas to pass through the rat cage 403, avoiding the existence of spaces in the rat cage 403 where gas does not circulate, thus affecting the accuracy of the experiment. The rat cage 403 is placed in the experimental chamber by a support member.

[0091] One technical solution: such as Figure 7 As shown, the rat poisoning chamber device 400 also includes a first support member 410 and a sludge collection tray 411. The rat cage 403 is placed in the experimental chamber via the first support member 410. Two first support members 410 are provided, respectively fixed to the inner wall of the poisoning chamber 401 and arranged opposite each other, with one end of the first support member 410 facing the side of the chamber door 402. Figure 8As shown, the first support member 410 has an inverted F-shaped structure, including a vertical mounting plate 4101 and a first horizontal plate 4102 and a second horizontal plate 4103 perpendicular to the side of the vertical mounting plate 4101. The vertical mounting plate 4101 is fixedly connected to the inner wall of the exposure chamber 401; a groove 4104 is formed between the first horizontal plate 4102 and the second horizontal plate 4103; the first horizontal plate 4102 is used to support the rat cage 403. The waste collection tray 411 is slidably disposed in the groove 4104. The waste collection tray 411 is used to collect the excrement produced by the rats to prevent the excrement from contaminating the chamber. The waste collection tray 411 adopts a drawer-type design, which makes it convenient for the experimenter to remove the waste collection tray 411 from the exposure chamber 401.

[0092] The structural design of the first support component 410 efficiently utilizes space and structure, simultaneously addressing the two core requirements of rat cage support and sludge tray guide rail in a single component. This simplifies the device structure and enhances its overall integrity and stability. The sludge tray 411 is slidably positioned within the groove 4104 formed by the first support component, achieving a drawer-like design. Experimenters can easily and quickly slide the sludge tray horizontally out of the chamber for cleaning or replacement without disassembling the rat cage or other components, greatly improving experimental efficiency and convenience. The groove 4104 is precisely defined by two horizontal plates (first horizontal plate 4102 and second horizontal plate 4103), providing precise sliding guidance and stable support for the sludge tray. The structure of the first support component 410 collectively enhances the performance of this rat poisoning chamber device in terms of experimental operability, ease of maintenance, contamination control, and long-term reliability.

[0093] Another technical solution: the structure of the support member can be an L-shaped structure similar to the second support member 312, and the support member is relatively fixed to the inner wall of the contaminated chamber 401, and the bottom of the rat cage 403 does not have a sludge collection tray 411. Figures 10 to 13 As shown, the rat poisoning chamber device 400 also includes a functional composite membrane 412 disposed inside the rat cage 403. The functional composite membrane 412 consists of two layers: a metal mesh substrate 4121 and a silicone film microporous coating 4122. The surface of the metal mesh substrate 4121 is coated with a conductive antibacterial coating (not shown in the figure). The metal mesh substrate 4121 is connected to the grounding rail of the chamber by a snap-fit ​​connection to form an electrostatic discharge path. When the rat moves in the rat cage 403, it generates static electricity, which can be discharged by the functional composite membrane 412.

[0094] Furthermore, the metal mesh substrate 4121 is woven from 316L medical-grade stainless steel wire or titanium alloy wire into a honeycomb mesh, with a mesh hole side length or hole diameter ≤0.3cm, providing high-strength support for its upper structure and ensuring that the mesh does not deform. The conductive antibacterial coating is a conductive polymer doped with nano-silver, and the conductive polymer is poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid (PEDOT / PSS), with a surface resistivity ≤10 Ω·cm. 6Ω eliminates static electricity buildup, improving the accuracy of gaseous pollutant detection; the conductive antibacterial coating can kill 99.9% of common pathogens (such as Escherichia coli and Staphylococcus aureus), inhibiting microorganisms, reducing the risk of cross-infection in rats, and reducing biological background noise. The surface microporous treatment of the 4122 silicone film microporous coating has a pore size of 5~10μm, and the microporous structure maintains air permeability, ensuring consistent exposure dose; silicone is non-toxic, corrosion-resistant, and easy to clean.

[0095] The honeycomb grid structure of the metal mesh substrate 4121 forms macroscopic airflow channels, reducing pressure loss. The microporous coating 4122 of the silicone film has a microporous surface treatment, eliminating microscopic turbulence. The metal mesh substrate 4121 and the microporous coating 4122 work together to achieve laminar gas flow, ensuring uniform airflow and avoiding deviations in exposure dose.

[0096] Nano-silver constitutes 3–10 wt% of the conductive polymer (poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid). A nano-silver content greater than 10 wt% can affect the continuity of the polymer and increase the risk of biotoxicity. A nano-silver content less than 3 wt% results in weak antibacterial properties.

[0097] The functional composite membrane 412 integrates three functions: antibacterial, conductive, and breathable. Its multifunctional integrated design and elimination of the root causes of experimental errors provide a revolutionary platform for high-precision toxicology research.

[0098] To illustrate the effect of the functional composite membrane 412, the utility model conducted the following experiment:

[0099] (a) Experimental group:

[0100] The rat cage includes a bottom plate and side panels, both of which have openings.

[0101] The cage contains a functional composite membrane with the aforementioned structure. The metal mesh substrate has a mesh aperture side length of 0.2 cm. The surface of the metal mesh substrate 4121 is coated with a conductive antibacterial coating, which is a conductive polymer doped with nano-silver. The conductive polymer is poly(3,4-ethylenedioxythiophene)-polystyrene sulfonic acid (PEDOT / PSS), and the nano-silver accounts for 5 wt% of the conductive antibacterial coating. The surface of the silicone film microporous coating 4122 is treated with micropores, with a pore size of 7 μm.

[0102] Rats were placed in cages, which were then placed inside an experimental chamber. The temperature inside the chamber was room temperature (25℃±1℃), and the relative humidity was 45%. A toxic gas, consisting of formaldehyde (2ppm) and benzo[a]pyrene aerosol (10μg / m³), was introduced into the chamber. 3 Rats were exposed in the experimental chamber for 28 days.

[0103] (II) Control Group:

[0104] The functional composite membrane in the experimental group was replaced with nonwoven fabric, with the same thickness as the functional composite membrane in the experimental group, and other conditions were the same as those in the experimental group.

[0105] Testing of peak static voltage inside the chamber: Static electricity inside the experimental chamber was detected using an electrostatic voltmeter. In this experiment, a Trek Model 347 electrostatic voltmeter was used, with multiple points placed on the surface of the rat cage (bottom plate / side wall) and inside the chamber (10~30 cm from the cage). Each group was repeated at least 3 times, and the average value was taken.

[0106] Detection of inflammatory factors in animal lungs: Lung tissue was collected, and 9 times the volume of PBS was added based on the tissue volume. The tissue was homogenized and subjected to three freeze-thaw cycles in liquid nitrogen. The tissue was centrifuged at 12000 rpm for 10 min, and the supernatant was the protein sample required for the experiment. The expression of the inflammatory factor IL-6 in rat lung tissue was detected by ELISA.

[0107] Testing for bacterial residue on the grid: The antimicrobial properties of the functional composite membrane surface were evaluated using microbial sampling and culture techniques. The testing process first required sterilization pretreatment of the experimental chamber and grid surface to reduce interference from environmental microorganisms. Sampling was performed using a cotton swab method; a sterile cotton swab dipped in PBS buffer was used to wipe a designated area before inoculating the sample onto an agar plate. The sampled plates were then incubated at 37°C for 24–48 hours. Afterward, a colony-forming units (CFU) counter was used to count the number of bacterial residues per unit area (CFU / cm²). To ensure data reliability, the experimental setup required controlled sampling time, a sterile operating environment, and a blank control.

[0108] The experimental data for the experimental group and the control group are shown in Table 1:

[0109] Table 1

[0110]

[0111] This technical solution achieves breakthroughs in several key performance indicators through innovative antibacterial conductive mesh design and biocompatible microporous coating structure. In terms of electrostatic control, by employing a nano-silver composite conductive coating and a full-path grounding design, the peak static voltage inside the chamber is significantly reduced from 16.2kV in traditional solutions to 0.2kV, a reduction of up to 98%, completely resolving the problem of distorted pollutant concentration monitoring caused by electrostatic adsorption of aerosol particles. Regarding biosafety, the innovative nano-silver antibacterial system controls the bacterial residue on the mesh surface to a GMP-level cleanliness level of <10 CFU / cm², effectively avoiding interference from microbial contamination on experimental results. Particularly noteworthy is the reduction in the abnormal increase of the inflammatory factor IL-6 in animal lungs from 320% to 85%, a decrease of 73%, demonstrating that this design significantly reduces the interference of non-toxic factors on experimental data, allowing the test results to more accurately reflect the biological effects of the toxic substance itself. These technical achievements mark a significant shift in toxicology chamber equipment from traditional "rough exposure" to "precise and controllable" methods, providing a more reliable and accurate experimental platform for toxicology research.

[0112] In the description of this utility model, it should be understood that the terms "upper", "bottom", "top", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0113] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature.

[0114] Finally, it should be noted that the above-described embodiments are merely specific implementations of this utility model, used to illustrate the technical solutions of this utility model, and not to limit it. The protection scope of this utility model is not limited thereto. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features, within the technical scope disclosed in this utility model. Such modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model, and should all be covered within the protection scope of this utility model.

Claims

1. A rat inhalation exposure testing device, characterized in that, It includes a toxic gas generator (300) and a rat toxic chamber device (400) connected in a closed loop via an intake pipe (100) and a return pipe (200). The contaminated gas generating device (300) includes: - The outer shell (301) has an inner cavity and an outer shell opening communicating with the inner cavity; - A gas generating chamber (302) is located inside the outer shell (301), with its chamber opening facing the same direction as the outer shell opening; the test plate (500) is placed inside the gas generating chamber (302); - A sealing door (303) is provided at the opening end of the outer shell (301) for opening and closing the inner cavity of the gas generating chamber (302); - Heating element (304), disposed in the inner cavity of gas generating chamber (302), used to heat the inner cavity of gas generating chamber (302); - Humidification assembly (305) for spraying humidifying water into the interior cavity of gas generating chamber (302); - The first temperature detection element (306) and the first humidity detection element (307) are respectively disposed on the side wall of the gas generating chamber (302), and the detection end is in the inner cavity of the gas generating chamber (302); The rat poison chamber device (400) includes: - The poison chamber (401) is equipped with an experimental chamber and a chamber opening that communicates with the experimental chamber; - The hatch (402) is located at the opening end of the poison chamber (401) and is used to open and close the experimental chamber; - Rat cage (403), located inside the experimental chamber of the poison treatment chamber (401); - The second temperature detector (404) and the second humidity detector (405) are respectively disposed on the side wall of the poisoning chamber (401), and the detection end is in the experimental chamber of the poisoning chamber (401); - An oxygen detection sensor (414) is installed in the experimental chamber of the poison treatment chamber (401); The intake pipe (100) and the return pipe (200) are respectively connected to the gas generation chamber (302) and the contaminated chamber (401).

2. The rat inhalation exposure testing device according to claim 1, characterized in that, A clean gas inlet pipe (308) is provided on the side wall of the gas generating chamber (302). One end of the clean gas inlet pipe (308) is connected to the inner cavity of the gas generating chamber (302), and the other end extends out of the outer shell (301) and is connected to the outside. An inlet control valve (309) is provided on the clean gas inlet pipe (308). The side wall of the exposure chamber (401) is provided with an exhaust pipe (406). One end of the exhaust pipe (406) is connected to the experimental chamber of the exposure chamber (401), and the other end extends out of the exposure chamber (401) and is connected to the outside. An exhaust control valve (407) is provided on the exhaust pipe (406).

3. The rat inhalation exposure testing device according to claim 2, characterized in that, A first gas sampling pipe (310) is provided on the side wall of the gas generating chamber (302). One end of the first gas sampling pipe (310) is connected to the inner cavity of the gas generating chamber (302), and the other end extends out of the outer shell (301) and is connected to the outside. A first gas sampling control valve (311) is provided on the first gas sampling pipe (310). The side wall of the exposure chamber (401) is provided with a second gas sampling pipe (408). One end of the second gas sampling pipe (408) is connected to the experimental chamber of the exposure chamber (401), and the other end extends out of the exposure chamber (401) and is connected to the outside. A second gas sampling control valve (409) is provided on the second gas sampling pipe (408).

4. The rat inhalation exposure testing device according to claim 1, characterized in that, An airflow control device (800) is provided on the air intake pipe (100). The airflow control device (800) includes a circulating fan (801), a wind speed sensor (802), and a gas cooling device (803). The gas cooling device (803) includes a heat sink (8031) mounted on the air inlet pipe (100) and a cooling fan (8032) attached to the heat sink (8031).

5. The rat inhalation exposure testing device according to claim 1, characterized in that, The contaminated gas generating device (300) also includes a centrifugal fan (313) and fins (314) disposed in the inner cavity of the gas generating chamber (302). The rat poison chamber device (400) also includes at least one stirring fan (413) disposed on the inner wall of the poison chamber (401).

6. The rat inhalation exposure testing device according to claim 1, characterized in that, The rat inhalation exposure test device also includes a controller (600) and a control panel (700); the controller (600) is located inside the housing (301), and the control panel (700) is located on the housing (301); The controller (600) is electrically connected to the heating element (304), the humidification component (305), the first temperature sensor (306), the first humidity sensor (307), the second temperature sensor (404), the second humidity sensor (405), the oxygen sensor (414), and the control panel (700).

7. The rat inhalation exposure testing device according to claim 1, characterized in that, The rat cage (403) includes a bottom plate (4031) and a side wall plate (4032), both of which have openings.

8. The rat inhalation exposure testing device according to claim 7, characterized in that, The rat poison chamber device (400) also includes: - Two first support members (410) are provided, which are fixed to the inner wall of the contaminated chamber (401) and arranged opposite to each other; the first support member (410) has an inverted F-shaped structure, including a vertical mounting plate (4101) and a first horizontal plate (4102) and a second horizontal plate (4103) perpendicular to the side of the vertical mounting plate (4101); the vertical mounting plate (4101) is fixedly connected to the inner wall of the contaminated chamber (401); a groove (4104) is formed between the first horizontal plate (4102) and the second horizontal plate (4103); the first horizontal plate (4102) is used to support the rat cage (403); - The sludge collection tray (411) is slidably disposed in the slide groove (4104).

9. The rat inhalation exposure testing device according to claim 7, characterized in that, The rat poison chamber device (400) also includes: - Functional composite membrane (412) is installed inside the rat cage (403); the functional composite membrane (412) consists of two layers: a metal mesh substrate (4121) and a silicone film microporous coating (4122); the surface of the metal mesh substrate (4121) is coated with a conductive antibacterial coating functional composite membrane; the functional composite membrane (412) is connected to the cabin grounding rail by a snap-fit ​​to form an electrostatic discharge path.

10. The rat inhalation exposure testing device according to claim 9, characterized in that, The metal mesh substrate (4121) is a honeycomb mesh woven from 316L medical stainless steel wire or titanium alloy wire, with a mesh hole side length or hole diameter ≤0.3cm; The surface micropore treatment of the silicone film microporous coating (4122) has a pore size of 5~10μm.

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