A pre-charged cell suspension cryovial with a protective agent

Abstract

The utility model discloses a cell suspension solution cryopreservation tube of prefilling protective agent, including cryopreservation tube main part, cryopreservation tube main part bottom fixedly connected with connecting seat, and the connecting seat bottom is connected with the placing seat, and the placing seat top is equipped with the placing groove, and the placing groove inside bottom is provided with the fixing base, and the connecting seat includes the locating plate, and the locating plate fixedly arranged in the cryopreservation tube main part bottom outside, and the locating plate one side is equipped with the locking groove, the utility model discloses through locating plate alignment and insert positioning groove, and the quick plug -in positioning connecting seat and fixing base, when locating plate inserts, and locating plate extrusion limit block top one side slope drives limit block to contract, and then through torsional spring and drive synchronous ring reset, thereby drive multiple limit block automatic synchronous reset, make locating boss automatic card into the inside limit slot, realize automatic limit fixed, and the quick fixed cryopreservation tube is placed in the placing groove, and the ice block is put into the placing groove, and when operating, the cryopreservation tube is cooled continuously, and the fixed cryopreservation tube is convenient for continuous operation.

Description

Technical Field

[0001] This utility model relates to the field of cryopreservation tube technology, and in particular to a cell suspension cryopreservation tube pre-filled with a protective agent. Background Technology

[0002] Cell cryopreservation is a technique that places cells in a low-temperature environment to reduce cellular metabolism for long-term storage. Cryotubes are one of the main methods of cell preservation, serving as containers for preserving or transferring bacterial strains in the laboratory. Pre-filled cryoprotectant cell suspensions are a more specific and advanced cell manipulation technique. This involves adding a cryoprotectant (most commonly DMSO) to the cryotubes before aliquoting the cell suspension. The cryotubes are pre-filled with the cryoprotectant and pre-chilled. When the cell suspension is added, the DMSO is instantly diluted, and the low temperature environment inhibits its toxicity, significantly shortening the cell's exposure time to high concentrations of DMSO at room temperature.

[0003] According to a published information on a cell cryopreservation tube (publication number: CN202020039086.X), the tube includes a tube body with a base on the bottom outer wall. The top outer wall of the base has a conical groove, and both sides of the inner wall of the conical groove have installation openings. The tube body is fixed by a fixed half-ring and a limiting block inside the base, which facilitates the placement of the cryopreservation tube. A sealing cap is also provided on the tube cap, which, in conjunction with a first spring, facilitates the sealing of the tube body. However, in the above preparation steps, the cryopreservation tube needs to be pre-frozen and pre-filled with cryoprotectant. Generally, this is done by placing the pre-filled tube on ice or in a 4°C freezer for pre-cooling before taking it out and placing the cryoprotectant. Pre-cooling in a freezer is cumbersome, and the cooling effect cannot be maintained after taking it out. Placing it on ice results in poor stability and is inconvenient for the next step. Utility Model Content

[0004] The purpose of this invention is to provide a cell suspension cryopreservation tube pre-filled with a protective agent. By quickly inserting the cryopreservation tube into the positioning groove through a positioning plate, rotating the cryopreservation tube causes the positioning protrusion at the bottom of the positioning plate to engage with the limiting groove inside the positioning groove, thereby limiting the connection seat and quickly fixing the cryopreservation tube inside the placement groove, thus solving the existing problems.

[0005] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution: This utility model is a cell suspension cryopreservation tube pre-filled with a protective agent, comprising: a cryopreservation tube body, a connecting seat fixedly connected to the bottom end of the cryopreservation tube body, a placement seat connected to the bottom end of the connecting seat, a placement groove opened at the top end of the placement seat, and a fixing seat provided at the bottom end of the placement groove. The connecting seat includes a positioning plate, which is fixedly installed on the outer side of the bottom of the cryopreservation tube body. A locking groove is provided on one side of the positioning plate, and a positioning protrusion is provided at the bottom end of the positioning plate. A positioning groove is provided at the top of the fixed seat, and a limit groove is provided on one side of the inner wall of the positioning groove. A limit block is movably provided on the other side of the inner wall of the positioning groove.

[0006] Furthermore, the number of positioning plates is set to six, the positioning plates are distributed in a circular array, the positioning grooves are correspondingly arranged with the positioning plates, the inner wall size of the positioning grooves is adapted to the outer surface size of the positioning plates, and guide arc surfaces are provided on both sides of the top of the positioning grooves.

[0007] Furthermore, the size of the limiting groove is adapted to the size of the positioning protrusion, and the positioning plate is engaged with the limiting groove through the positioning protrusion.

[0008] Furthermore, a slot is provided at the center of the top of the fixed base, and a spring telescopic rod is provided in the slot. A corresponding insertion block is provided at the center of the bottom of the connecting base. The outer surface of the insertion block is adapted to the size of the inner wall of the slot. The insertion block is movably connected to the slot through the spring telescopic rod.

[0009] Furthermore, the top of the cryopreservation tube is threaded with a cap, and the outer surface of the cap is provided with anti-slip texture.

[0010] Furthermore, an annular suction cup is provided on the outer side of the bottom of the placement seat, a rotating block is movably connected to the outer side of the placement seat, a movable block is movably connected to the inside of the placement seat, a connecting rod is fixedly provided at the bottom of the movable block, and a number of connecting rods are provided and distributed in a circular array, with the bottom end of the connecting rod connected to the top of the annular suction cup.

[0011] Furthermore, a synchronizing ring is fixedly connected to the bottom end of the limiting block via a connecting rod. A torsion spring is provided at the bottom end of the synchronizing ring. The synchronizing ring is movably disposed in the empty slot of the placement seat. One end of the torsion spring is fixedly connected to the synchronizing ring, and the other end of the torsion spring is fixedly connected to the bottom end of the empty slot inside the placement seat. The synchronizing ring is movably connected to the empty slot via the torsion spring.

[0012] This utility model has the following beneficial effects: This invention uses a guide arc surface to guide the positioning plate when it is aligned and inserted into the positioning groove, facilitating quick and easy insertion and positioning. When the positioning plate is inserted, it presses against the inclined surface on one side of the top of the limiting block, causing the limiting block to retract. Then, a torsion spring drives the synchronous ring to reset, thereby causing multiple limiting blocks to automatically and synchronously reset. This allows the positioning protrusion to automatically engage with the inner limiting groove, achieving automatic limiting and fixing. This quickly fixes the cryopreservation tube inside the placement groove. Ice blocks are placed in the placement groove, and the cryopreservation tube is continuously cooled during operation. The fixed cryopreservation tube facilitates continuous operation.

[0013] This utility model features an annular suction cup on the outer side of the bottom of a placement base, a rotating block movably connected to the outer side of the placement base, and a movable block movably connected inside the placement base. A connecting rod is fixedly installed at the bottom of the movable block, and several connecting rods are arranged in a circular array. The bottom of the connecting rod is connected to the top of the annular suction cup. The annular suction cup allows the placement base to be stably placed on a table. The rotating block drives the internal movable block to move, and the movable block controls the suction of the annular suction cup through the connecting rod, making it convenient and quick to fix and release the position of the placement base.

[0014] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall separate structure of this utility model; Figure 2 This is a schematic diagram of the main structure of the cryopreservation tube of this utility model; Figure 3 This is a schematic diagram of the connection structure between the connector and the fixed base of this utility model; Figure 4 This is a schematic diagram of the fixing base structure of this utility model. Figure 5 This is a schematic diagram of the overall bottom view of the structure of this utility model; Figure 6 This is a schematic diagram of the internal structure of the connection between the connector and the fixing seat of this utility model; Figure 7 This is a schematic diagram of the internal structure of the placement base of this utility model.

[0017] As shown in the diagram: 1. Cryopreservation tube body; 2. Tube cap; 3. Connecting seat; 301. Positioning plate; 302. Locking groove; 303. Positioning protrusion; 304. Insertion block; 4. Placement seat; 401. Annular suction cup; 402. Rotating block; 403. Movable block; 404. Connecting rod; 5. Placement groove; 6. Fixed seat; 601. Positioning groove; 602. Limiting groove; 603. Slot; 604. Spring telescopic rod; 605. Limiting block; 606. Synchronization ring; 607. Torsion spring. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] Please see Figure 1-7 The cryopreservation tube body 1 has a connecting seat 3 fixedly connected to the bottom end of the cryopreservation tube body 1. The connecting seat 3 has a placement seat 4 connected to the bottom end of the connecting seat 3. The placement seat 4 has a placement groove 5 at the top end and a fixing seat 6 is provided at the bottom end of the placement groove 5. The connecting seat 3 includes a positioning plate 301, which is fixedly installed on the bottom outer side of the cryopreservation tube body 1. A locking groove 302 is provided on one side of the positioning plate 301, and a positioning protrusion 303 is provided at the bottom end of the positioning plate 301. A positioning groove 601 is provided at the top of the fixed seat 6. A limit groove 602 is provided on one side of the inner wall of the positioning groove 601, and a limit block 605 is movably provided on the other side of the inner wall of the positioning groove 601.

[0020] There are six sets of positioning plates 301 arranged in a circular array. Positioning grooves 601 are correspondingly arranged with the positioning plates 301. The inner wall size of the positioning groove 601 is adapted to the outer surface size of the positioning plate 301. Guide arc surfaces are provided on both sides of the top of the positioning groove 601. The guide arc surfaces guide the positioning plate 301 when it is aligned and inserted into the positioning groove 601, which facilitates quick and easy insertion and positioning.

[0021] The size of the limiting groove 602 is adapted to the size of the positioning protrusion 303. The positioning plate 301 is engaged with the limiting groove 602 through the positioning protrusion 303. The positioning protrusion 303 is rotated to engage with the limiting groove 602 inside the positioning groove 601 for fixation.

[0022] A slot 603 is provided at the center of the top of the fixed base 6, and a spring telescopic rod 604 is provided inside the slot 603. A corresponding insert block 304 is provided at the center of the bottom of the connecting base 3. The outer surface of the insert block 304 is adapted to the size of the inner wall of the slot 603. The insert block 304 is movably connected to the slot 603 through the spring telescopic rod 604. When the positioning plate 301 is inserted into the positioning groove 601, the insert block 304 is inserted into the corresponding slot 603 at the center of the top of the fixed base 6. The spring telescopic rod 604 resets and drives the insert block 304 to move upward, thereby securing the positioning protrusion 303 in the limiting groove 602.

[0023] The cryopreservation tube body 1 has a tube cap 2 threadedly connected to its top end. The outer surface of the tube cap 2 is provided with anti-slip texture. The anti-slip texture facilitates the rotation and opening of the tube cap 2 and prevents slippage during rotation.

[0024] An annular suction cup 401 is provided on the outer side of the bottom of the placement base 4. A rotating block 402 is movably connected to the outer side of the placement base 4. A movable block 403 is movably connected inside the placement base 4. A connecting rod 404 is fixedly provided at the bottom of the movable block 403. Several connecting rods 404 are provided and distributed in a circular array. The bottom of the connecting rod 404 is connected to the top of the annular suction cup 401. The annular suction cup 401 can stably place the placement base 4 on the table. The rotating block 402 drives the internal movable block 403 to move. The movable block 403 controls the suction of the annular suction cup 401 through the connecting rod 404, so as to conveniently and quickly fix and release the position of the placement base 4.

[0025] The bottom end of the limiting block 605 is fixedly connected to the synchronous ring 606 via a connecting rod. The bottom end of the synchronous ring 606 is provided with a torsion spring 607. The synchronous ring 606 is movably disposed in the empty groove of the placement seat 4. One end of the torsion spring 607 is fixedly connected to the synchronous ring 606, and the other end of the torsion spring 607 is fixedly connected to the bottom end of the empty groove inside the placement seat 4. The synchronous ring 606 is movably connected to the empty groove via the torsion spring 607. One side of the bottom end of the limiting block 605 is provided with an inclined surface. When the positioning plate 301 is inserted into the positioning groove 601, it causes the limiting block 605 to retract. Then, the torsion spring 607 causes the synchronous ring 606 to reset, thereby causing multiple limiting blocks 605 to automatically and synchronously reset, so that the positioning protrusion 303 automatically engages in the inner limiting groove 602 and limits it.

[0026] A specific application of this embodiment is as follows: In use, the placement seat 4 is placed in the desired position. By rotating the rotating block 402, the rotating block 402 drives the movable block 403 to rise through the thread. The movable block 403 drives the annular suction cup 401 to generate suction through the connecting rod 404, thereby fixing the position of the placement seat 4. After pre-cooling, the tube cover 2 is opened, and the positioning plate 301 at the bottom of the cryopreservation tube body 1 is inserted into the positioning groove 601 in the placement seat 4. The positioning plate 301 presses the inclined surface on one side of the top of the limiting block 605, causing the limiting block 605 to retract. Then, the torsion spring 607 drives the synchronous ring 606 to reset, thereby driving multiple limiting blocks 605 to move from... The synchronous reset allows the positioning protrusion 303 to automatically engage with the inner limiting groove 602, achieving automatic limiting and fixing, and quickly securing the cryovial body 1. Ice is placed in the placement groove 5 to pre-cool the cryovial body 1. An appropriate amount of preservative is placed inside the cryovial, followed by the cell suspension. The cap 2 is closed. By rotating the cryovial body 1, the positioning plate 301 is aligned with the positioning groove 601. The spring telescopic rod 604 resets, causing the insertion block 304 to move upward, lifting the cryovial body 1, thus quickly removing the cryovial. Finally, it is gently inverted and mixed 2-3 times before being placed in the freezer for freezing.

[0027] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0028] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A cell suspension cryopreservation tube pre-filled with a cryoprotectant, characterized in that, include: The cryopreservation tube body (1) has a connecting seat (3) fixedly connected to the bottom end of the cryopreservation tube body (1), a placement seat (4) is connected to the bottom end of the connecting seat (3), a placement groove (5) is opened at the top end of the placement seat (4), and a fixing seat (6) is provided at the bottom end of the placement groove (5). The connecting seat (3) includes a positioning plate (301), which is fixedly installed on the bottom outer side of the cryopreservation tube body (1). A locking groove (302) is provided on one side of the positioning plate (301), and a positioning protrusion (303) is provided at the bottom end of the positioning plate (301). A positioning groove (601) is provided at the top end of the fixing seat (6). A limiting groove (602) is provided on one side of the inner wall of the positioning groove (601), and a limiting block (605) is movably provided on the other side of the inner wall of the positioning groove (601).

2. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 1, characterized in that, The number of positioning plates (301) is set in six groups, and the positioning plates (301) are arranged in a ring array. The positioning grooves (601) are arranged correspondingly to the positioning plates (301). The inner wall size of the positioning groove (601) is adapted to the outer surface size of the positioning plate (301). The top two sides of the positioning groove (601) are provided with guide arc surfaces.

3. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 1, characterized in that, The size of the limiting groove (602) is adapted to the size of the positioning protrusion (303), and the positioning plate (301) is engaged with the limiting groove (602) through the positioning protrusion (303).

4. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 3, characterized in that, The top center of the fixed base (6) is provided with a slot (603), and a spring telescopic rod (604) is provided in the slot (603). The bottom center of the connecting base (3) is provided with a corresponding insert (304). The outer surface of the insert (304) is adapted to the size of the inner wall of the slot (603). The insert (304) is movably connected to the slot (603) through the spring telescopic rod (604).

5. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 1, characterized in that, The cryopreservation tube body (1) has a tube cap (2) threaded to its top end, and the outer surface of the tube cap (2) is provided with anti-slip texture.

6. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 1, characterized in that, An annular suction cup (401) is provided on the outer side of the bottom end of the placement seat (4). A rotating block (402) is movably connected to the outer side of the placement seat (4). A movable block (403) is movably connected inside the placement seat (4). A connecting rod (404) is fixedly provided at the bottom end of the movable block (403). There are several connecting rods (404) arranged in a circular array. The bottom end of the connecting rod (404) is connected to the top of the annular suction cup (401).

7. The cell suspension cryopreservation tube pre-filled with a protective agent according to claim 1, characterized in that, The bottom end of the limiting block (605) is fixedly connected to a synchronizing ring (606) via a connecting rod. A torsion spring (607) is provided at the bottom end of the synchronizing ring (606). The synchronizing ring (606) is movably disposed in the empty groove of the placement seat (4). One end of the torsion spring (607) is fixedly connected to the synchronizing ring (606), and the other end of the torsion spring (607) is fixedly connected to the bottom end of the empty groove inside the placement seat (4). The synchronizing ring (606) is movably connected to the empty groove via the torsion spring (607).

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