Cellular fluid replacement aid

Abstract

The application discloses a cell supplementing liquid auxiliary device, which comprises a base serving as a basic support structure, a clamping seat arranged in the middle of the base and provided with a plurality of clamping grooves suitable for tubes with different diameters, a cover with a lifting function arranged on the clamping seat and used for axially fixing the tubes, a liquid storage mechanism arranged on one side of the base and comprising a mounting frame and a liquid storage cylinder fixed to the mounting frame, wherein the liquid storage cylinder is connected with a pipeline for supplementing liquid, and a shaking mechanism installed between the base and the clamping seat and used for driving the clamping seat to move back and forth. The shaking mechanism drives the clamping seat to shake, so that the liquid in the tubes is shaken uniformly.

Description

Technical Field

[0001] This utility model relates to the field of cell culture auxiliary equipment processing, specifically to a cell fluid replenishment auxiliary device. Background Technology

[0002] In cell biology research and biopharmaceutical fields, fluid replenishment (addition of nutrient solution + cell suspension) during cell culture is a crucial step in maintaining cell viability and proliferation, and its precision directly affects the reliability of experimental results. Mixing during cell replenishment primarily relies on manual operation. The specific procedure is as follows: the experimenter holds a test tube containing cell suspension in one hand and a bottle containing replenishment solution in the other, pouring the solution into the test tube by tilting it. After initial mixing, the test tube is manually shaken before being poured into a culture bag (or culture medium) for further replenishment. This mixing method, because it is entirely manual, suffers from poor operational stability, thus affecting the cell culture effect.

[0003] Referring to existing technology CN220846055U, a cell culture bag replenishment device is disclosed. Through the cooperation of a base, shaker, auxiliary support, and replenishment connection assembly, it solves the problem of spillage caused by hand tremors when manually lifting culture medium bottles. Its core structure includes: the vertical part of the auxiliary support forms a 135° angle with the first inclined part, and the first inclined part forms a 90° angle with the second inclined part, used to fix a 1L culture medium bottle; the replenishment connection assembly connects the culture bag and the culture medium bottle through tubing, and a manual latching switch controls the flow rate.

[0004] The replenishment process is controlled by a switch (clamping structure) in the tubing, preventing manual tipping. The shaker agitates the cell culture bag, mixing the replenished fluid with the culture medium inside and ensuring uniform cell distribution. However, the existing technology is designed for culture bags, not test tubes, as its structure is suited for flexible culture bags and is completely unsuitable for rigid test tubes. Applying this technology to test tubes would be problematic because it only places the culture bag on the shaker, lacking a rigid fixing component for the test tubes. Given the rigidity and high center of gravity of the test tubes, the probability of them rolling off during shaking is significantly higher than the risk of the culture bag detaching.

[0005] Furthermore, the flexible material of the culture bag can withstand large amplitude vibrations, while the test tube contains a small amount of liquid. Large amplitude vibrations from the shaker can cause the liquid to impact the test tube wall, generating air bubbles and damaging cell structure. Therefore, developing a dedicated liquid-assisted shaking device tailored to the structural characteristics of the test tube is key to solving these technical challenges. Utility Model Content

[0006] The present invention aims to provide a cell rehydration aid device, specifically a shaker for rehydration aid that is tailored to the structural characteristics of test tubes.

[0007] A cell rehydration aid device includes: a base as a basic support structure; a mounting bracket located in the middle of the base, the mounting bracket having multiple slots for fitting test tubes of different diameters; a pressure cap with lifting function located on the mounting bracket for axially fixing the test tubes; a liquid storage mechanism located on one side of the base, including a mounting frame and a liquid storage cylinder fixed to the mounting frame, the liquid storage cylinder being connected to a rehydration pipeline; and a shaking mechanism installed between the base and the mounting bracket for driving the mounting bracket to move back and forth.

[0008] The working principle and beneficial effects of this utility model: The base serves as a support structure, and the holder secures the test tube via a slot, ensuring radial stability. Liquid is added to the test tube by opening the tubing, and then the cap presses the test tube in place axially. The shaking mechanism drives the holder to vibrate, mixing the liquid within the test tube. The cap provides stable axial pressure, which, combined with the radial constraint of the slot, creates a rigid, integral connection between the test tube and the holder. During the holder's vibration, the test tube will not slip due to centrifugal force or vibration (such as vertical movement or radial offset), but will completely follow the preset trajectory of the shaking mechanism, improving mixing efficiency.

[0009] The optimized design includes a silicone anti-slip layer on the inner wall of the card slot.

[0010] In this optimized configuration, a pump is installed between the pipeline and the storage tank. The pump precisely controls the infusion rate and volume.

[0011] The optimized shaking mechanism includes a moving platform and a motor. The card holder is fixed on the moving platform, and the base is fixed with a slide rail. The moving platform is horizontally slidably connected through guide shoes and the slide rail. The bottom of the moving platform is provided with a mounting groove, and horizontal racks are fixed on opposite sides of the mounting groove. The motor drives an incomplete gear that alternately meshes with the racks on both sides.

[0012] The motor drives the incomplete gear to rotate, and the teeth of the incomplete gear alternately mesh with the racks on both sides of the mounting slot at the bottom of the moving platform. When the teeth of the incomplete gear mesh with the rack on one side, it pushes the moving platform to slide to one side along the slide rail; when the teeth disengage from the rack on that side, the incomplete gear continues to rotate and meshes with the rack on the other side, driving the platform to slide in the opposite direction.

[0013] This linear motion causes less damage to adherent cells (such as neurons) and results in a higher survival rate, making it particularly suitable for rapid mixing in 1-5 mL test tubes.

[0014] Optimized, the bottom of the pressure cap is fixed with a rubber gasket. The rubber gasket is made of food-grade silicone, which has high elasticity and sealing properties. When it comes into contact with the top of the test tube, it undergoes elastic deformation, fills tiny gaps, and absorbs vibration energy.

[0015] In an optimized configuration, the card holder is equipped with a cylinder that drives the cap to move toward the card slot. The cylinder drives the cap to move axially up and down, ensuring stable contact between the cap and the top of the test tube. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of a cell rehydration aid device according to this application; Figure 2 for Figure 1 A schematic diagram of the structure after removing the pressure cap; Figure 3 This is a schematic diagram of the structure after the card slot has been moved to the right; Figure 4 for Figure 1 Schematic diagram of the structure of the intermediate pressure cover; Figure 5 A schematic diagram showing the connection between a rack and an incomplete gear; Figure 6 for Figure 5 A schematic diagram of the structure in which an incomplete gear rotates to the rack on the other side; Figure 7 for Figure 1 A structural diagram with the mobile platform removed.

[0017] The reference numerals in the accompanying drawings include: 1. Liquid storage cylinder; 2. Support; 3. Moving platform; 4. Card holder; 5. Slide rail; 6. Blind groove; 7. Pipeline; 8. Cap; 9. Liquid pump; 10. Cylinder; 11. Test tube; 12. Guide shoe; 13. Mounting groove; 14. Rack; 15. Incomplete gear; 16. Motor. Detailed Implementation

[0018] The following detailed description illustrates the specific implementation method: Example: Refer to Figures 1 to 7 As shown, firstly, two THK HSR15A linear guide rails 5 are fixed to the aluminum alloy base using M4 bolts, and thick rubber buffer pads are installed at both ends of the guide rails 5. Guide shoes 12 are fixed to both sides of the bottom of the 6061 aluminum moving platform 3 using locating pins and M3 bolts to cooperate with the guide rails 5. A 42SHD0217-24B type motor 16 is fixed inside the base, and an incomplete gear 15 is fixedly installed on the output shaft. A mounting groove 13 is provided at the center of the bottom of the moving platform 3, and racks 14 are symmetrically fixed to the mounting groove 13 by bolts. The incomplete gear 15 and the racks 14 on both sides mesh alternately.

[0019] The ABS material card holder 4 is fixed to the center of the moving platform 3 by M5 bolts. The surface is machined with card slots, and the inner wall of the card slot is pasted with a 2mm thick silicone anti-slip layer.

[0020] An aluminum bracket 2 is installed on the left side of the base. A 50mL liquid storage cylinder 1 is fixed on the top of the bracket 2. Liquid is injected by a BT100M pump 9. A pipe 7 is connected between the inlet of the pump 9 and the liquid storage cylinder 1 and the outlet of the peristaltic pump. When not in use (or during shaking), the pipe 7 is hidden by a blind groove 6 set on the card seat 4.

[0021] An SMC CXSM25-50 cylinder 10 is installed on the top of the base, and a pressure cap 8 is fixedly installed at the end of the piston rod. A 5mm thick silicone pad is pasted on the bottom of the pressure cap 8.

[0022] Insert test tube 11 into the slot to secure it. Start the pump 9 to draw liquid from storage tank 1 into pipe 7. Insert pipe 7 into test tube 11 to replenish the liquid. After replenishment, turn off pump 9 and hide pipe 7 in blind groove 6. Start cylinder 10 to move cap 8 downwards to clamp and secure test tube 11 along its axis. Then, drive incomplete gear 15 to rotate via motor 16. The teeth of incomplete gear 15 alternately mesh with racks 14 on both sides of mounting groove 13 at the bottom of moving platform 3. When the teeth of incomplete gear 15 mesh with one rack 14, it pushes moving platform 3 to slide to the right along slide rail 5. When the teeth disengage from that rack 14, incomplete gear 15 continues to rotate and meshes with the other rack 14, driving moving platform 3 to slide in the opposite direction. The back-and-forth alternating movement of moving platform 3 achieves the purpose of mixing the liquid in test tube 11.

[0023] After shaking is complete, motor 16 stops working, cylinder 10 drives cap 8 to detach from test tube 11, and test tube 11 can be taken out. The mixed solution in test tube 11 can be poured into a culture bag or culture medium for incubation.

[0024] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A cell rehydration aid device, characterized in that, include: Includes: a base, serving as the basic support structure; A card holder, located in the middle of the base, is provided with multiple slots for fitting test tubes of different diameters; A lifting cap is mounted on a mounting bracket for axially fixing test tubes; A liquid storage mechanism is located on one side of the base and includes a mounting frame and a liquid storage cylinder fixed to the mounting frame. The liquid storage cylinder is connected to a pipeline for replenishing liquid. The shaking mechanism is installed between the base and the card holder to drive the card holder to move back and forth.

2. The cell rehydration aid device according to claim 1, characterized in that: The inner wall of the card slot is provided with a silicone anti-slip layer.

3. The cell rehydration aid device according to claim 2, characterized in that: A pump is installed between the pipeline and the storage tank.

4. The cell rehydration aid device according to claim 3, characterized in that: The shaking mechanism includes a moving platform and a motor. The card holder is fixed on the moving platform, and the base is fixed with a slide rail. The moving platform is horizontally slidably connected through guide shoes and the slide rail. The bottom of the moving platform is provided with a mounting groove, and horizontal racks are fixed on opposite sides of the mounting groove. The motor drives an incomplete gear that alternately meshes with the racks on both sides.

5. The cell rehydration aid device according to claim 4, characterized in that: A rubber pad is fixed to the bottom of the pressure cap.

6. The cell rehydration aid device according to claim 5, characterized in that: The card holder is fixed with a cylinder that drives the pressure cover to move toward the card slot.

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