Microscope retrofit module based on simultaneous excitation of multiple lasers
By modifying the microscope module, the excitation and collection modules are integrated, and a four-channel laser input and dual-band spectral testing are designed. This solves the problems of space occupation and signal interference caused by multiple laser excitations in the microscope, and improves the accuracy and stability of spectral detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- HUBEI ZHONGWEI PHOTOELECTRIC TECH CO LTD
- Filing Date
- 2025-09-15
- Publication Date
- 2026-07-28
AI Technical Summary
Existing commercial microscopes suffer from problems such as large space occupation, high equipment complexity, optical path mismatch and signal interference in multi-laser excitation combined with spectral detection functions, resulting in poor accuracy and repeatability of spectral detection.
By installing and modifying the module, the excitation module and the collection module are integrated into one unit. The design includes four laser inputs and two signal collections. Combined with appropriate beam splitting elements and filters, dual-band spectral testing is achieved.
It improves the stability of the equipment and the accuracy of spectral detection, enabling in-depth understanding of the microstructure and optical properties of materials, and supports spectral detection with multiple lasers working simultaneously.
Smart Images
Figure CN224569349U_ABST
Abstract
Description
Technical Field
[0001] The embodiments of this utility model belong to the field of microscopic spectral detection technology, and more specifically, relate to a microscope modification module based on simultaneous excitation by multiple lasers. Background Technology
[0002] Microscopic imaging, as a key technology for exploring the microscopic world, plays an indispensable role in many cutting-edge fields such as biomedicine, materials science, and nanotechnology. With the deepening of scientific research, extremely stringent requirements are being placed on the precision, depth, speed, and multi-dimensional information acquisition capabilities of microscope imaging. Among these, spectral detection, as an important means of obtaining characteristics such as the chemical composition and molecular structure of samples, has become a new research hotspot due to its integration with imaging technology.
[0003] In terms of spectral detection, a single light source can only excite a limited spectral response of the sample, making it difficult to fully obtain the spectral characteristics of the sample under different wavelengths of excitation, which greatly limits the in-depth analysis of the sample. The multi-laser simultaneous excitation technology has emerged to provide strong support for the combination of spectral detection and imaging. Compared with single laser excitation, multi-laser excitation can use multiple laser beams of different wavelengths to act on the sample at the same time, which can not only enrich the imaging information, but also fully excite the spectral response of the sample.
[0004] Currently, commercial microscopes on the market have significant shortcomings in multi-laser excitation combined with spectral detection capabilities. Most microscopes use multiple independent input ports to achieve multi-laser introduction, which not only greatly occupies the limited physical space of the microscope and increases the complexity and size of the equipment, but also places extremely high demands on the scalability of the imaging platform, making system integration much more difficult. Especially when integrated with the spectral detection module, problems such as optical path mismatch and signal interference are prone to occur. In addition, traditional microscopes lack effective coordination mechanisms in optical path switching, synchronization control, and acquisition and processing of spectral signals under different laser excitations when performing multi-laser excitation combined with spectral detection. This leads to interference between different optical paths when multiple lasers work simultaneously for spectral detection, resulting in unstable spectral signals and seriously affecting the accuracy and repeatability of spectral detection. Summary of the Invention
[0005] To address the aforementioned deficiencies or improvement needs of existing technologies, this utility model provides a microscope modification module based on simultaneous excitation by multiple lasers. The modification module, mounted on top of a module support column, integrates the excitation and collection modules. The support column connects the modification module to the microscope body. The four laser inputs designed in the excitation module can excite the responses of materials with complex optical properties at different frequency bands. The two signal collection channels in the collection module, along with appropriately designed beam-splitting elements and corresponding filters, enable dual-band spectral testing.
[0006] To achieve the above objectives, this utility model provides a microscope modification module based on simultaneous excitation by multiple lasers, comprising:
[0007] The microscope body is fixedly mounted on the optical stage, and the module support column is located at a fixed position on the optical stage;
[0008] The modification module, located at the top of the module support column and at the top of the microscope body, includes a beam splitter at the top of the microscope body, an adjustable mirror at the top of the beam splitter and coaxial with the beam splitter, a second light-transmitting hole on the outside of the modification module and corresponding to the beam splitter, a first light-transmitting hole on the outside of the modification module and corresponding to the adjustable mirror, a third light-transmitting hole on the upper layer of the outside of the modification module, and a fourth light-transmitting hole on the lower layer of the outside of the modification module.
[0009] The excitation module located inside the modification module includes a first fiber optic adapter respectively disposed inside the modification module and located at each of the third light-transmitting holes, and a lens located at a distance behind each of the first fiber optic adapters and coaxial with it.
[0010] The collection module located inside the modification module includes a second optical fiber adapter respectively disposed inside the modification module and located at each of the fourth light-transmitting holes, and an achromatic lens located behind each of the second optical fiber adapters.
[0011] Furthermore, the microscope body also includes a main structure, an imaging module disposed at the bottom of the beam splitter, an objective lens disposed on the main structure and located at the bottom of the imaging module, and a triaxial displacement sample stage connected to the main structure and located below the objective lens.
[0012] Furthermore, the objective lens, the adjustable mirror, and the beam splitter are coaxial.
[0013] Furthermore, the excitation module also includes a first reflecting mirror, a front dichroic mirror, a middle dichroic mirror, and a rear dichroic mirror located behind the lenses and coaxial with each lens.
[0014] Furthermore, the excitation module also includes a mirror assembly with one end coaxial with the first mirror and the other end coaxial with the adjustable mirror.
[0015] Furthermore, the collection module also includes a first filter and a second filter located behind the achromatic lenses and coaxial with each achromatic lens.
[0016] Furthermore, the collection module also includes a second reflecting mirror located behind the first filter and coaxial with the achromatic lens, and a collection dichroic mirror located behind the second filter and coaxial with the achromatic lens.
[0017] Furthermore, both the second reflecting mirror and the collecting dichroic mirror are coaxial with the beam splitter.
[0018] Furthermore, the modification module has a two-layer structure, with the excitation module located on the upper layer and the collection module located on the lower layer.
[0019] In summary, compared with the prior art, the above-described technical solution conceived by this utility model can achieve the following beneficial effects:
[0020] 1. The module of this utility model integrates the excitation module and the collection module by installing a modification module on the top of the module support column. The module support column connects the modification module to the microscope body. The four laser inputs designed in the excitation module can excite the response of materials with complex optical properties in different frequency bands. The two signal collections designed in the collection module, along with the reasonable design of beam splitting elements and corresponding filters, can realize dual-band spectral testing.
[0021] 2. The module of this utility model collimates the light emitted from the optical fiber by adjusting the distance between the first optical fiber adapter and the lens, and realizes laser beam combining by reflecting and transmitting the laser through the first reflecting mirror and each dichroic mirror. By adjusting the reflecting mirror group, the adjustable reflecting mirror reflects the excitation light onto the objective lens.
[0022] 3. The module of this utility model introduces the signal light returned from the objective lens into the collection module through a beam splitter, splits the light signal through a dichroic mirror, removes part of the light signal through a second filter, converges the light signal through an achromatic lens, and receives the light signal through a second fiber optic adapter. By rationally designing the dichroic mirror, the first filter, and the second filter, dual-band spectral testing can be achieved. Attached Figure Description
[0023] Figure 1 This is a schematic diagram of the microscope modification module based on simultaneous excitation by multiple lasers, according to an embodiment of this utility model.
[0024] Figure 2 This is a schematic diagram of a microscope modification module based on simultaneous excitation by multiple lasers, according to an embodiment of the present invention.
[0025] Figure 3 This is a schematic diagram of the external structure of the microscope modification module collection module based on simultaneous excitation by multiple lasers, according to an embodiment of this utility model.
[0026] Figure 4 This is a schematic diagram of the internal structure of the excitation module of the microscope modification module based on simultaneous excitation by multiple lasers, according to an embodiment of this utility model.
[0027] Figure 5 This is a schematic diagram of the internal structure of the multi-channel collection module of the microscope modification module based on simultaneous excitation by multiple lasers, according to an embodiment of this utility model.
[0028] In all the accompanying drawings, the same reference numerals denote the same technical features, specifically: 1-collection module, 11-adjustable reflector, 12-beam splitter, 13-first light-transmitting aperture, 14-second light-transmitting aperture, 15-third light-transmitting aperture, 16-fourth light-transmitting aperture, 2-microscope body, 21-imaging module, 22-main structure, 23-objective lens, 24-triaxial displacement sample stage, 3-module support column, 4-excitation module, 41-first fiber optic adapter, 42-lens, 43-first reflector, 44-front dichroic mirror, 45-middle dichroic mirror, 46-rear dichroic mirror, 47-mirror group, 5-collection module, 51-second fiber optic adapter, 52-achromatic lens, 53-first filter, 54-second filter, 55-second reflector, 56-collection dichroic mirror. Detailed Implementation
[0029] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.
[0030] In the description of the embodiments of this utility model, it should be noted that if terms such as "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," or "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the utility model product is in use, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, terms such as "first," "second," and "third" are only used to distinguish descriptions and should not be construed as indicating or implying relative importance.
[0031] Furthermore, the use of terms such as "horizontal," "vertical," and "sag" does not imply that the component must be absolutely horizontal or suspended, but rather that it can be slightly tilted. For example, "horizontal" simply means that its direction is more horizontal relative to "vertical," and does not mean that the structure must be completely horizontal, but can be slightly tilted.
[0032] In the description of the embodiments of this utility model, "a plurality of" means at least two.
[0033] In the description of the embodiments of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0034] This novel embodiment provides a microscope modification module based on simultaneous excitation by multiple lasers, such as... Figure 1 , Figure 2 As shown, the system includes a modification module 1, a microscope body 2, module support columns 3, an excitation module 4, and a collection module 5. The microscope body 2 is fixed to the optical stage, and multiple module support columns 3 are fixed to the optical stage and located on both sides of the microscope body 2. The modification module 1 is installed on the top of the module support columns 3. The modification module 1 is also located on the top of the microscope body 2 and connected to the imaging module 21. The excitation module 4 and the collection module 5 are respectively installed inside the modification module 1. The integrated installation of the excitation module 4 and the collection module 5 avoids the complexity and volume of the equipment caused by assembling numerous independent modules, and improves the stability of the equipment operation. The excitation module 4 is designed with four laser inputs. By reasonably selecting the beam splitting element, it can support the simultaneous input of four lasers. For materials with complex optical properties, it can excite their responses in different frequency bands. Combined with spectral detection, it can gain a deeper understanding of the relationship between the internal microstructure and optical properties of the material, and analyze the composition distribution and chemical state of the material. The collection module 5 is designed with two signal collections. By reasonably designing the beam splitting element and the corresponding filter, it can achieve the desired effect. This invention enables dual-band spectral testing, such as simultaneously performing narrow-band, high-resolution Raman signal testing and wide-band fluorescence signal testing with relatively low resolution requirements, facilitating the spectral analysis of dynamic phenomena such as rapid physiological processes and chemical reactions. The module of this invention integrates the excitation and collection modules through a modified module mounted on top of a module support column. The module support column connects the modified module to the microscope body. The four laser inputs designed in the excitation module can excite the responses of materials with complex optical properties at different frequency bands. The two signal collection channels in the collection module, along with the rationally designed beam-splitting elements and corresponding filters, enable dual-band spectral testing.
[0035] Specifically, such as Figure 3As shown, in the modification module 1, the external structure of the modification module 1 includes an adjustable reflector 11, a beam splitter 12, a first light-transmitting aperture 13, a second light-transmitting aperture 14, a third light-transmitting aperture 15, and a fourth light-transmitting aperture 16. The modification module 1 has an upper and lower double-layer structure. The upper layer of the modification module 1 houses the excitation module 4, and the lower layer houses the collection module 5. The adjustable reflector 11 is installed on the outside of the upper layer of the modification module 1, reflecting the excitation light to the objective lens 23. The beam splitter 12 is installed on the outside of the lower layer of the modification module 1, located at the bottom of the adjustable reflector 11, and splits the beam. Mirror 12 introduces the returned signal light from objective lens 23 into the light collection path. Adjustable mirror 11 and beam splitter 12 must be coaxial with objective lens 23 in microscope body 2. First light-passing hole 13 is located at the connection between adjustable mirror 11 and the outer shell of the modification module. Second light-passing hole 14 is located at the connection between beam splitter 12 and the outer shell of the modification module. Third light-passing hole 15 is opened on the outer side of modification module 1. Third light-passing hole 15 is located on the upper layer of modification module 1. Fourth light-passing hole 16 is opened on the outer side of modification module 1. Third light-passing hole 15 and fourth light-passing hole 16 are on the same side. Fourth light-passing hole 16 is located on the lower layer of modification module 1.
[0036] Specifically, such as Figure 1 As shown, the microscope body 2 includes an imaging module 21, a main structure 22, an objective lens 23, and a triaxial displacement sample stage 24. The imaging module 21 is installed on the top of the microscope body 2. The main structure 22 is the frame structure for mounting other components of the microscope body 2. The imaging module 21 is installed in the main structure 22. The objective lens 23 is installed on the main structure 22 and is located at the bottom of the imaging module 21. The triaxial displacement sample stage 24 is installed on the main structure 22 and is located at the bottom of the objective lens 23. The sample to be observed needs to be placed on the triaxial displacement sample stage 24 and located in the center of the objective lens 23.
[0037] Specifically, such as Figure 4As shown, the excitation module 4 includes a first fiber optic adapter 41, a lens 42, a first reflector 43, a front dichroic mirror 44, a middle dichroic mirror 45, a rear dichroic mirror 46, and a reflector group 47. The excitation module 4 is located inside the upper layer of the modification module 1. The four sets of first fiber optic adapters 41 are respectively fixed inside the corresponding third light-transmitting holes 15. A lens 42 is mounted coaxially on each first fiber optic adapter 41. There is a gap between each first fiber optic adapter 41 and its corresponding lens 42. Adjusting this gap allows for the control of the light emitted from the fiber optic cable. For alignment, the first reflecting mirror 43, the front dichroic mirror 44, the middle dichroic mirror 45, and the rear dichroic mirror 46 are all installed behind the corresponding lenses 42 and are ensured to be coaxial with each other. The reflecting mirror group 47 is located on the other side of the upper layer of the modified module 1. After being collimated by the lens 42, the fiber optic light output is reflected and transmitted by the first reflecting mirror 43, the front dichroic mirror 44, the middle dichroic mirror 45, and the rear dichroic mirror 46 respectively, thereby realizing laser beam combining. Then, the reflecting mirror group 47 is adjusted, and the excitation light is reflected onto the objective lens 23 by the adjustable reflecting mirror 11. In this utility model module, the fiber optic light output is collimated by adjusting the distance between the first fiber optic adapter and the lens. Laser beam combining is achieved by the reflection and transmission of the laser by the first reflecting mirror and each dichroic mirror. By adjusting the reflecting mirror group, the adjustable reflecting mirror reflects the excitation light onto the objective lens.
[0038] Specifically, such as Figure 5 As shown, the collection module 5 includes a second fiber optic adapter 51, an achromatic lens 52, a first filter 53, a second filter 54, a second reflector 55, and a collecting dichroic mirror 56. The collection module 5 is located inside the lower layer of the modified module 1. Two sets of second fiber optic adapters 51 are fixed inside each of the fourth light-passing holes 16. An achromatic lens 52 is installed coaxially at each second fiber optic adapter 51. Behind the achromatic lens 52, a first filter 53 and a second filter 54, coaxial with each second fiber optic adapter 51, are also installed. The signal light returned from the objective lens 23 is introduced into the collection module 5 through the beam splitter 12. Then, the light signal is split by the collecting dichroic mirror 56. A portion of the light signal is filtered by the second filter 54, and then converged by the achromatic lens 52 before finally entering the second fiber optic adapter 51 for light signal reception. The module of this utility model introduces the signal light returned from the objective lens into the collection module through a beam splitter, splits the light signal through a dichroic mirror, removes part of the light signal through a second filter, converges the light signal through an achromatic lens, and receives the light signal through a second fiber optic adapter. By rationally designing the dichroic mirror, the first filter, and the second filter, dual-band spectral testing can be achieved.
[0039] In an optional embodiment, in the excitation module 4, the first reflector 43 is a silver film reflector, the front dichroic mirror 44 is a 1100 nm long-pass type (reflects light below 1100 nm and transmits light above 1100 nm), the middle dichroic mirror 45 is a 750 nm long-pass type, and the rear dichroic mirror 46 is a 400 nm long-pass type. After the design, the four sets of laser inlets are divided into wavelength bands as λ > 1100 nm, 1100 > λ > 750 nm, 750 > λ > 400 nm, and 400 nm > λ. After being incident on each of them, the four excitation beams can be combined and finally focused on the same excitation position on the sample.
[0040] In another embodiment of this utility model, a microscope modification module based on simultaneous excitation by multiple lasers is provided, comprising the following steps:
[0041] Place the microscope body 2 on the optical stage, then install the module support column 3 in a fixed position on the optical stage, and then install the modification module 1.
[0042] Check whether the microscope body 2 can form a uniform and bright field of view, and adjust the magnification of the microscope body 2 to the required magnification.
[0043] Place the sample to be observed in the center of the stage and align it with objective lens 23.
[0044] Open the camera to observe the micro-area image, and move the sample using the triaxial displacement sample stage 24 to find the appropriate test site.
[0045] Configure 1-4 types of lasers according to usage requirements, and match the wavelength of the introduced laser to the input band of excitation module 4, and connect them through optical fiber.
[0046] The laser is turned on to introduce multiple beams of light simultaneously, which work together and the excitation site is observed through a microscope.
[0047] Two output optical signals are introduced into different analytical instruments via optical fibers to achieve dual-band spectral testing.
[0048] After finishing the operation, turn off the laser, remove the sample, save the test data, and reset the microscope body 2. Then, put the microscope body 2 into the special dust cover.
[0049] In summary, this microscope modification module based on simultaneous excitation by multiple lasers can provide in-depth understanding of the relationship between the internal microstructure and optical properties of materials, as well as analyze the composition distribution and chemical state of materials. In addition, it can also perform dual-band spectral testing.
[0050] Those skilled in the art will readily understand that the above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A microscope modification module based on simultaneous excitation by multiple lasers, characterized in that, include: The microscope body (2) is fixedly installed on the optical stage, and the module support column (3) is set at a fixed position on the optical stage. The modification module (1) located on top of the module support column (3) and on top of the microscope body (2) includes a beam splitter (12) located on top of the microscope body (2), an adjustable mirror (11) located on top of the beam splitter (12) and coaxial with the beam splitter (12), a second light-transmitting hole (14) located outside the modification module (1) and corresponding to the beam splitter (12), a first light-transmitting hole (13) located outside the modification module (1) and corresponding to the adjustable mirror (11), a third light-transmitting hole (15) located on the upper layer of the outer side of the modification module (1), and a fourth light-transmitting hole (16) located on the lower layer of the outer side of the modification module (1). The excitation module (4) located inside the modification module (1) includes a first fiber optic adapter (41) located inside the modification module (1) and at each of the third light-transmitting holes (15), and a lens (42) located a distance away from each of the first fiber optic adapters (41) and coaxial. The collection module (5) located inside the modification module (1) includes a second fiber optic adapter (51) located inside the modification module (1) and at each of the fourth light-transmitting holes (16), and an achromatic lens (52) located behind each of the second fiber optic adapters (51).
2. The microscope modification module based on simultaneous excitation by multiple lasers according to claim 1, characterized in that, The microscope body (2) also includes a main structure (22), an imaging module (21) located at the bottom of the beam splitter (12), an objective lens (23) located on the main structure (22) and at the bottom of the imaging module (21), and a triaxial displacement sample stage (24) connected to the main structure (22) and located below the objective lens (23).
3. The microscope modification module based on simultaneous excitation by multiple lasers according to claim 2, characterized in that, The objective lens (23), the adjustable mirror (11), and the beam splitter (12) are coaxial.
4. A microscope modification module based on simultaneous excitation by multiple lasers according to any one of claims 1-3, characterized in that, The excitation module (4) also includes a first reflecting mirror (43) located behind the lens (42) and coaxial with each lens (42), a front dichroic mirror (44), a middle dichroic mirror (45), and a rear dichroic mirror (46).
5. A microscope modification module based on simultaneous excitation by multiple lasers according to claim 4, characterized in that, The excitation module (4) also includes a mirror group (47) with one end coaxial with the first mirror (43) and the other end coaxial with the adjustable mirror (11).
6. A microscope modification module based on simultaneous excitation by multiple lasers according to any one of claims 1-3, characterized in that, The collection module (5) also includes a first filter (53) and a second filter (54) located behind the achromatic lens (52) and coaxial with each achromatic lens (52).
7. A microscope modification module based on simultaneous excitation by multiple lasers according to claim 6, characterized in that, The collection module (5) further includes a second reflector (55) located behind the first filter (53) and coaxial with the achromatic lens (52), and a collection dichroic mirror (56) located behind the second filter (54) and coaxial with the achromatic lens (52).
8. A microscope modification module based on simultaneous excitation by multiple lasers according to claim 7, characterized in that, The second reflecting mirror (55) and the collecting dichroic mirror (56) are both coaxial with the beam splitter (12).
9. A microscope modification module based on simultaneous excitation by multiple lasers according to any one of claims 1-3, characterized in that, The modification module (1) is divided into an upper and lower double-layer structure. The excitation module (4) is located on the upper layer of the modification module (1), and the collection module (5) is located on the lower layer of the modification module (1).