An on-line sterile filtration device for cell culture media

Abstract

The utility model relates to the technical field of cell culture, and specifically discloses an online sterile filtering device for cell culture medium, which comprises: a liquid preparation mechanism; a filtering mechanism, which comprises a first filter and a second filter, the first filter is connected to the liquid preparation mechanism through a first pipeline, the first filter is connected to the second filter through a second pipeline, the second filter is externally connected to a third pipeline, and the first filter and the second filter are provided with filter cores; the third pipeline is connected to a first reactor; a functional mechanism, which comprises a steam pipeline connected to the first pipeline; and a control mechanism connected to the liquid preparation mechanism, the first filter, the second filter and the steam pipeline. The utility model realizes complete sterility of the second filter, and overcomes the defects of pollution hazards and incomplete cleaning in the online filtering of the cell culture medium in the prior art.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, and in particular to an online sterile filtration device for cell culture medium. Background Technology

[0002] In the fields of biopharmaceuticals, cell therapy, and biomedical research, the sterility of cell culture media is one of the core requirements for their application in cell culture. Microbial contamination (such as bacteria, fungi, or mycoplasma) in the culture medium will directly affect cell viability, experimental results, or the quality of biological products, and may even lead to batch spoilage and significant economic losses. Therefore, terminal aseptic treatment of the culture medium is an indispensable and critical step in the production process.

[0003] In existing technologies, cell culture media are usually directly used without sterilization and disinfection steps, which can easily lead to contamination of the culture media. In addition, existing technologies also lack online control methods for cell culture media, which is not convenient enough. Utility Model Content

[0004] (a) Technical problems to be solved The problem to be solved by this invention is to provide an online aseptic filtration device for cell culture medium, so as to achieve complete sterility of the second-stage filter and overcome the pollution risks and incomplete cleaning defects of the existing online filtration of cell culture medium.

[0005] (II) Technical Solution To solve the aforementioned technical problem, the first aspect of this utility model is an online aseptic filtration device for cell culture medium, comprising: Liquid preparation mechanism; The filtration mechanism includes a first filter and a second filter. The first filter is connected to the liquid preparation mechanism via a first pipe, and the first filter is connected to the second filter via a second pipe. The second filter is externally connected to a third pipe. Both the first filter and the second filter have filter elements for filtering the prepared liquid. The third pipeline is connected to the first reactor; A functional mechanism, the functional mechanism including a steam pipe, the steam pipe being connected to the first pipe; The control mechanism is connected to the liquid dispensing mechanism, the first filter, the second filter, and the steam pipeline.

[0006] As described above, in the online aseptic filtration device for cell culture medium, optionally, the steam pipe and the first pipe are respectively equipped with a first on / off valve, a second on / off valve and a third on / off valve, the first on / off valve, the second on / off valve and the third on / off valve controlling the opening / closing of the steam pipe and the first pipe respectively.

[0007] As described above, in the online aseptic filtration device for cell culture medium, pressure gauges may optionally be provided on the first filter and the second filter.

[0008] As described above, in the online aseptic filtration device for cell culture medium, the third pipe may optionally be provided with a steam outlet.

[0009] As described above, the online aseptic filtration device for cell culture medium may optionally include a diaphragm valve on the first filter and the second filter.

[0010] (III) Beneficial Effects The online aseptic filtration device for cell culture medium provided by this utility model has the following beneficial effects: (1) By controlling the steam pipeline to input steam into the first pipeline, the second pipeline, the filter mechanism and the third pipeline through the control mechanism, the internal parts are sterilized and disinfected to ensure sterilization and reduce the interference of water vapor to subsequent parts.

[0011] (2) By opening the first on / off valve at the liquid preparation mechanism, the culture medium liquid is input, pre-filtered by the first filter, and further filtered by the second filter. The input of the filtered culture medium liquid into the first reactor or the second reactor is controlled by opening the fourth or fifth on / off valve. This design can control the on / off of different lines through the control mechanism, improving convenience. Attached Figure Description

[0012] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a schematic diagram of an online aseptic filtration device for cell culture medium according to the present invention.

[0014] The component names corresponding to the various labels in the figure are as follows: 1. Liquid dispensing mechanism; 2. Filtration mechanism; 21. First filter; 22. Second filter; 23. Second pipeline; 24. Third pipeline; 25. Filter element; 26. First reactor; 28. Pressure gauge; 3. Functional mechanism; 31. Steam pipeline; 33. First pipeline; 34. First on / off valve; 35. Second on / off valve; 36. Third on / off valve; 4. Control mechanism; 6. Diaphragm valve; 8. Steam outlet. Detailed Implementation

[0015] The present application will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0016] The following specific examples illustrate the implementation of this application. Those skilled in the art can easily understand other advantages and effects of this application from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. This application can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this application. It should be noted that, in the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0017] It should be noted that various aspects of embodiments within the scope of the appended claims are described below. It will be apparent that the aspects described herein can be embodied in a wide variety of forms, and any particular structure and / or function described herein is merely illustrative. Based on this application, those skilled in the art will understand that one aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspects set forth herein can be used to implement the device and / or practice the method. Additionally, this device and / or method can be implemented using structures and / or functionalities other than one or more of the aspects set forth herein.

[0018] It should also be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of this application. The drawings only show the components related to this application and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the form, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0019] Additionally, specific details are provided in the following description to facilitate a thorough understanding of the examples. However, those skilled in the art will understand that practice can be carried out without these specific details.

[0020] The technical solutions provided by the various embodiments of this application are described below with reference to the accompanying drawings.

[0021] See Figure 1 This invention provides an online aseptic filtration device for cell culture media, comprising: a solution preparation mechanism 1, a filtration mechanism 2, a functional mechanism 3, and a control mechanism 4. The solution preparation mechanism 1 supplies culture medium liquid to the filtration mechanism 2. The filtration mechanism 2 provides filtration support for the culture medium liquid to remove impurities. The functional mechanism 3 is used for external steam connection. The control mechanism 4 controls the operation of the device.

[0022] Furthermore, the liquid preparation mechanism 1 can transport the culture medium liquid into the filtration device.

[0023] exist Figure 1 In an optional embodiment, the filtration mechanism 2 includes a first filter 21 and a second filter 22. The first filter 21 is connected to the liquid preparation mechanism 1 through a first pipe 33, and the first filter 21 and the second filter 22 are connected through a second pipe 23. The second filter 22 is externally connected to a third pipe 24. The first filter 21 and the second filter 22 have filter elements 25 for filtering the liquid preparation.

[0024] The first filter 21 is used for preliminary filtration of the culture medium solution, and the second filter 22 is used for further filtration of the culture medium solution filtered by the first filter 21. The first filter 21 and the second filter 22 are connected by a second pipe 23. The liquid preparation mechanism 1 supplies liquid to the filtration mechanism 2 through the first pipe 33.

[0025] It should be noted that pressure gauges 28 are installed on both the first filter 21 and the second filter 22. The pressure gauges 28 are used to measure the pressure in the first filter 21 and the second filter 22. Both the first filter 21 and the second filter 22 are externally connected to pressure relief valves 7. When the pressure gauge 28 detects that the pressure in the first filter 21 or the second filter 22 is too high, the pressure relief valves 7 open to ensure the safety of the filtration mechanism 2.

[0026] Furthermore, the third pipe 24 is connected to the first reactor 26, wherein the capacity of the first reactor 26 is variable. In this embodiment, it is a 100L reactor. In other embodiments of the present invention, the capacity of the first reactor 26 may be, but is not limited to, 100L.

[0027] Furthermore, both the first and second filters are equipped with diaphragm valves. These diaphragm valves effectively prevent external bacteria from entering the filtration system, thus ensuring sterility.

[0028] Functional mechanism 3 includes a steam pipe 31 connected to the first pipe 33. The steam pipe 31 can input steam into the first pipe 33, the second pipe 23, and the third pipe 24, whereby the steam can disinfect the pipes and the filter mechanism 2.

[0029] Furthermore, a steam outlet 8 is provided on the third pipe 24, so that steam can flow out from the steam outlet 8.

[0030] Furthermore, the steam pipe 31 and the first pipe 33 are respectively equipped with a first on / off valve 34, a second on / off valve 35 and a third on / off valve 36, which control the opening / closing of the steam pipe 31 and the first pipe 33 respectively.

[0031] Control mechanism 4 is connected to liquid dispensing mechanism 1, first filter 21, second filter 22 and steam pipeline 31 respectively.

[0032] The present invention discloses an online aseptic filtration device for cell culture medium, the specific steps of which are as follows: The control mechanism 4 controls the steam pipe 31 to input steam into the first pipe 33, the second pipe 23, the filter mechanism 2, and the third pipe 24. This sterilizes and disinfects the interior, ensuring aseptic treatment and reducing the impact of steam on subsequent processes.

[0033] By opening the first on / off valve 34 at the liquid preparation mechanism 1, the culture medium liquid is input, pre-filtered by the first filter 21, and further filtered by the second filter 22, thereby controlling the input of the filtered culture medium liquid into the first reactor 26.

[0034] The same or similar parts between the various embodiments in this specification can be referred to mutually. Each embodiment focuses on describing the differences from other embodiments.

[0035] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. An online aseptic filtration device for cell culture medium, characterized in that, include: Liquid preparation mechanism (1); The filtration mechanism (2) includes a first filter (21) and a second filter (22). The first filter (21) is connected to the liquid preparation mechanism (1) through a first pipe (33), and the first filter (21) and the second filter (22) are connected through a second pipe (23). The second filter (22) is externally connected to a third pipe (24). The first filter (21) and the second filter (22) have filter elements (25) for filtering the liquid preparation. The third pipe (24) is connected to the first reactor (26); Functional mechanism (3), the functional mechanism (3) includes a steam pipe (31), the steam pipe (31) being connected to the first pipe (33); The control mechanism (4) is connected to the liquid dispensing mechanism (1), the first filter (21), the second filter (22) and the steam pipe (31).

2. The online aseptic filtration device for cell culture medium as described in claim 1, characterized in that, The steam pipe (31) and the first pipe (33) are respectively equipped with a first on / off valve (34), a second on / off valve (35) and a third on / off valve (36), and the first on / off valve (34), the second on / off valve (35) and the third on / off valve (36) control the opening / closing of the steam pipe (31) and the first pipe (33).

3. The online aseptic filtration device for cell culture medium as described in claim 1, characterized in that, Pressure gauges (28) are provided on the first filter (21) and the second filter (22).

4. The online aseptic filtration device for cell culture medium as described in claim 1, characterized in that, The third pipe (24) is provided with a steam outlet (8).

5. The online aseptic filtration device for cell culture medium as described in claim 1, characterized in that, The first filter (21) and the second filter (22) are also provided with diaphragm valves (6).

Images