A centrifuge adapted for the preparation of centrifuge tubes from clinical cell mitochondria extraction.

Abstract

This invention relates to the field of medical device technology, and particularly to a centrifuge adapted for the preparation of centrifuge tubes for clinical cell mitochondrial extraction. The centrifuge includes: a centrifuge body, a centrifuge lid hinged to one side of the centrifuge body, a platelet detection device fixedly disposed on the side of the centrifuge lid away from the centrifuge body, and a liquid aspiration device rotatably disposed on the side of the centrifuge lid near the centrifuge body. A lid locking mechanism is fixedly disposed on the side of the centrifuge body near the centrifuge lid, and a locking hook is fixedly disposed on the side of the centrifuge lid near the centrifuge body, the locking hook matching the lid locking mechanism. This invention avoids the variability caused by human operation during multiple supernatant extractions, reduces the possibility of contamination from human operation, ensures the sterility and component stability of the extraction process, and greatly improves the efficiency and success rate of mitochondrial extraction.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a centrifuge adapted for the preparation of centrifuge tubes for the extraction of mitochondria from clinical cells. Background Technology

[0002] In the field of cell mitochondrial extraction and preparation, a centrifuge tube has been designed to meet the needs of clinical applications. Its application number is 2024223079394. This centrifuge tube requires the cell / mitochondrial precipitate to be located far away from the liquid outlet. However, mitochondrial extraction often requires multiple extractions of supernatant. Traditional manual operation methods have significant variability, making it difficult to guarantee the accuracy and consistency of each extraction. Furthermore, manual operation inevitably introduces the risk of contamination, which undoubtedly increases the difficulty and complexity of clinical applications and makes it difficult to meet the strict clinical requirements for sterility, stability, and efficiency in cell mitochondrial extraction and preparation.

[0003] In summary, to simplify the operation process and achieve clinical mitochondrial extraction in a more convenient and stable manner, an automatic liquid aspiration device is equipped on the centrifuge to achieve a fully automated integrated process from cell to mitochondrial extraction. This avoids manual operation after the centrifuge tubes are placed in the centrifuge tubes, minimizing the risk of product contamination and component variability. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a centrifuge adapted for the preparation of centrifuge tubes from clinical cell mitochondrial extraction.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0006] A centrifuge adapted for the preparation of centrifuge tubes from clinical cell mitochondrial extraction includes: a centrifuge body, a centrifuge cover hinged to one side of the centrifuge body, a platelet detection device fixedly disposed on the side of the centrifuge cover away from the centrifuge body, and a liquid aspiration device rotatably disposed on the side of the centrifuge cover close to the centrifuge body.

[0007] The centrifuge body is provided with a cover locking mechanism on the side near the centrifuge cover, and the centrifuge cover is provided with a locking hook on the side near the centrifuge body, the locking hook matching the cover locking mechanism;

[0008] An electric push rod is hinged in the centrifuge body, and the output end of the electric push rod is hinged to the side of the centrifuge cover near the centrifuge body.

[0009] The liquid suction device includes: a mounting bracket fixedly disposed on one side near the centrifuge body, a plurality of liquid suction pumps fixedly disposed in the mounting bracket, and a waste liquid recovery pump fixedly disposed in the mounting bracket;

[0010] The bottom of the suction pump is fixedly provided with a suction pipe and a discharge pipe, and the bottom of the waste liquid recovery pump is fixedly provided with a waste liquid recovery pipe and a waste liquid discharge pipe.

[0011] The waste liquid recovery pipe is connected to the discharge pipe of the suction pump that is spatially adjacent to it.

[0012] The suction tube and the discharge tube are matched with the centrifuge tube.

[0013] Furthermore, the centrifuge body includes: a casing, a centrifuge rotor, a program interaction panel, a power socket, a motor, and a power switch;

[0014] The centrifuge chamber is fixedly installed inside the chassis, the centrifuge rotor is rotatably installed in the centrifuge chamber, the program interaction panel is fixedly installed on one side of the chassis, the power socket is fixedly installed on the outside of the chassis, and the power switch is fixedly installed on one side of the chassis.

[0015] The motor is fixedly mounted on the upper part of the bottom plate in the chassis, and the output shaft of the motor passes through the bottom of the centrifuge chamber and is fixedly connected to the bottom of the centrifuge rotor.

[0016] The upper part of the centrifuge rotor is provided with several centrifuge tube slots, and the centrifuge tubes for the preparation of clinical cell mitochondria extraction are placed in the centrifuge tube slots.

[0017] Furthermore, the casing on the side of the chassis away from the program interaction panel is a heat sink, and the heat sink has several heat dissipation holes.

[0018] Furthermore, a platelet detection device interactive panel is fixedly provided in the platelet detection device;

[0019] The interactive panel of the platelet detection device is electrically connected to the platelet detection device.

[0020] Furthermore, the aspiration pump, the waste liquid recovery pump, the platelet detection device, the program interaction panel, the cover locking mechanism, the electric push rod, the motor, the power switch, and the power socket are all electrically connected.

[0021] Furthermore, both the suction pump and the waste liquid recovery pump are diaphragm pumps;

[0022] The outlet end of the suction tube is connected to the suction valve in the suction pump, and the inlet end of the discharge tube is connected to the discharge valve in the suction pump.

[0023] The outlet end of the waste liquid recovery pipe is connected to the suction valve in the waste liquid recovery pump, and the inlet end of the waste liquid discharge pipe is connected to the discharge valve in the waste liquid recovery pump.

[0024] Furthermore, the mounting bracket is cross-shaped, the waste liquid recovery pump is fixedly located at the central intersection of the mounting bracket, the mounting bracket includes four branch rods, and the suction pump is fixedly located on the four branch rods of the mounting bracket.

[0025] Furthermore, it also includes: machine legs fixedly disposed at the bottom of the centrifuge body;

[0026] The legs are located at the bottom of the centrifuge body near the four corners.

[0027] Furthermore, the chassis also includes: a shock-absorbing spring; one end of the shock-absorbing spring is fixedly disposed on the upper part of the base plate, and the other end of the shock-absorbing spring is fixedly disposed on the bottom of the centrifuge chamber;

[0028] The shock-absorbing spring is covered with a flexible spring sleeve.

[0029] The present invention adopts the above technical solution and has the following technical effects compared with the prior art:

[0030] This invention achieves an integrated process from cell to mitochondrial extraction through a liquid aspiration device. The aspiration pump and waste liquid recovery pump, matched with the centrifuge tubes through a rational pipeline layout, can perform quantitative liquid aspiration and waste liquid recovery according to a preset program sequence. This avoids the inconsistencies caused by human operation during multiple supernatant extractions, reduces the possibility of contamination from human operation, ensures the sterility and component stability of the extraction process, and greatly improves the efficiency and success rate of mitochondrial extraction, while also reducing the difficulty and cost of clinical application. The platelet detection device located on the centrifuge lid uses impedance method to detect platelet-related parameters such as quantity and volume. The centrifuge body and the centrifuge lid are matched by a lid locking mechanism and hook, and the opening and closing are controlled by an electric push rod, ensuring the sealing and safety of the equipment during operation and preventing dangerous situations caused by accidental opening. The shock-absorbing springs and flexible spring sleeves installed inside the casing effectively reduce the damage to internal components caused by vibration generated during motor operation and centrifugation, extending the service life of the equipment. They also reduce the impact of vibration on the accuracy of detection and extraction results, ensuring the stable operation of the centrifuge. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the axial side structure of this utility model;

[0032] Figure 2 This is a schematic diagram of the rear structure of this utility model;

[0033] Figure 3 This is a schematic diagram of the liquid suction device in this utility model;

[0034] Figure 4 This is a schematic diagram of the internal structure of the centrifuge body in this utility model;

[0035] The reference numerals in the attached figures are:

[0036] Centrifuge body, 1; Centrifuge lid, 2; Platelet detection device, 4; Liquid suction device, 5; Lid locking mechanism, 10; Locking hook, 15; Electric push rod, 16; Mounting bracket, 51; Suction pump, 52; Waste liquid recovery pump, 53; Suction tube, 521; Drain tube, 522; Waste liquid recovery tube, 531; Waste liquid drainage tube, 532; Chassis, 11; Centrifuge rotor, 12; Program interaction panel, 3; Power socket, 8; Motor, 21; Power switch, 6; Centrifuge chamber, 19; Heat sink, 14; Platelet detection device interaction panel, 7; Legs, 9; Shock-absorbing spring, 20; Base plate, 22; Spring sleeve, 23; Centrifuge tube trough, 13. Detailed Implementation

[0037] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0038] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0039] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention.

[0040] Example

[0041] This embodiment provides a centrifuge adapted for the preparation of centrifuge tubes from clinical cell mitochondrial extraction, comprising: a centrifuge body 1, a centrifuge cover 2 hinged to one side of the centrifuge body 1, a platelet detection device 4 fixedly disposed on the side of the centrifuge cover 2 away from the centrifuge body 1, and a liquid aspiration device 5 rotatably disposed on the side of the centrifuge cover 2 close to the centrifuge body 1.

[0042] The centrifuge body 1 is fixedly provided with a cover locking mechanism 10 on the side near the centrifuge cover 2, and the centrifuge cover 2 is fixedly provided with a locking hook 15 on the side near the centrifuge body 1, and the locking hook 15 matches the cover locking mechanism 10.

[0043] An electric push rod 16 is hinged in the centrifuge body 1. The output end of the electric push rod 16 is hinged to the side of the centrifuge cover 2 near the centrifuge body 1. The centrifuge body 1 also includes legs 9 fixedly disposed at the bottom of the centrifuge body 1. The legs 9 are located at the bottom of the centrifuge body 1 near the four corners.

[0044] The liquid suction device 5 includes: a mounting bracket 51 fixedly disposed on one side near the centrifuge body 1, a plurality of liquid suction pumps 52 fixedly disposed in the mounting bracket 51, and a waste liquid recovery pump 53 fixedly disposed in the mounting bracket 51.

[0045] The bottom of the suction pump 52 is fixedly provided with a suction pipe 521 and a discharge pipe 522, and the bottom of the waste liquid recovery pump 53 is fixedly provided with a waste liquid recovery pipe 531 and a waste liquid discharge pipe 532; wherein, the waste liquid discharge pipe 532 is connected to the outside of the centrifuge body 1.

[0046] The waste liquid recovery pipe 531 is connected to the discharge pipe 522 of the suction pump 52 which is spatially adjacent to it, and the suction pipe 521 and the discharge pipe 522 are matched with centrifuge tubes.

[0047] The centrifuge body 1 includes: a casing 11, a centrifuge rotor 12, a program interaction panel 3, a power socket 8, a motor 21, and a power switch 6.

[0048] The centrifuge chamber 19 is fixedly disposed inside the chassis 11, the centrifuge rotor 12 is rotatably disposed in the centrifuge chamber 19, the program interaction panel 3 is fixedly disposed on one side of the chassis 11, the power socket 8 is fixedly disposed on the outside side of the chassis 11, and the power switch 6 is fixedly disposed on one side of the chassis 11.

[0049] The motor 21 is fixedly mounted on the upper part of the bottom plate 22 in the casing 11, and the output shaft of the motor passes through the bottom of the centrifuge chamber 19 and is fixedly connected to the bottom of the centrifuge rotor 12.

[0050] The centrifuge rotor 12 has several centrifuge tube slots 13 on its upper part, and the centrifuge tubes for the preparation of clinical cell mitochondria extraction are placed in the centrifuge tube slots 13.

[0051] The casing of the chassis 11 on the side away from the program interaction panel 3 is a heat sink 14, and the heat sink 14 has a plurality of heat dissipation holes.

[0052] The chassis 11 further includes a shock-absorbing spring 20; one end of the shock-absorbing spring 20 is fixedly disposed on the upper part of the base plate 22, and the other end of the shock-absorbing spring 20 is fixedly disposed on the bottom of the centrifuge chamber 19.

[0053] The shock-absorbing spring 20 is covered with a flexible spring sleeve 23.

[0054] The platelet detection device 4 is fixedly equipped with a platelet detection device interaction panel 7;

[0055] The platelet detection device interactive panel 7 is electrically connected to the platelet detection device 4.

[0056] The aspiration pump 52, the waste liquid recovery pump 53, the platelet detection device 4, the program interaction panel 3, the cover locking mechanism 10, the electric push rod 16, the motor 21, the power switch 6, and the power socket 8 are all electrically connected.

[0057] The liquid suction pump 52 and the waste liquid recovery pump 53 are both diaphragm pumps, and their working principle is based on the reciprocating motion of the diaphragm to realize the suction and discharge of liquid.

[0058] The outlet end of the suction pipe 521 is connected to the suction valve of the suction pump 52, and the inlet end of the discharge pipe 522 is connected to the discharge valve of the suction pump 52. The outlet end of the waste liquid recovery pipe 531 is connected to the suction valve of the waste liquid recovery pump 53, and the inlet end of the waste liquid discharge pipe 532 is connected to the discharge valve of the waste liquid recovery pump 53. The suction pump 52 and the waste liquid recovery pump 53 use diaphragm pumps to perform liquid suction and waste liquid recovery in an orderly and stable manner, avoiding liquid backflow or leakage.

[0059] The mounting bracket 51 is cross-shaped, the waste liquid recovery pump 53 is fixedly disposed at the central intersection position of the mounting bracket 51, the mounting bracket 51 includes four branch rods, and the suction pump 52 is fixedly disposed on the four branch rods of the mounting bracket 51.

[0060] In a preferred embodiment, the platelet detection device 4 is a platelet analyzer.

[0061] As a preferred embodiment, the lid locking mechanism 10 adopts the locking mechanism with application number CN201310462821.2 and patent name "Lid Locking Mechanism".

[0062] In a preferred embodiment, the liquid suction device 5 is rotatably mounted on the side of the centrifuge cover (2) near the centrifuge body (1) via a turntable bearing.

[0063] When in use, plug the power cord into the power socket 8 of the centrifuge body 1 and ensure that the power connection is reliable and not loose. Turn on the power switch 6. At this time, the program interaction panel 3 of the centrifuge and the platelet detection device interaction panel 7 will light up. Place the centrifuge tubes containing the samples one by one into the centrifuge tube slots 13 opened on the upper part of the centrifuge rotor 12 of the centrifuge body 1. Ensure that the centrifuge tubes are placed stably without tilting or shaking. The design of the centrifuge tube slots 13 is adapted to the shape of the centrifuge tubes to ensure the stability and safety of the centrifuge tubes during centrifugation. Before centrifuging the samples, draw a small amount of blood from the centrifuge tubes and add it to the platelet detection device 4 for detection. Then, select the pre-set mitochondrial extraction centrifugation program on the program interaction panel 3 and set the centrifugation speed, time and temperature parameters.

[0064] After the centrifugation program is started, the motor 21 starts working, driving the centrifugal rotor 12 to rotate at high speed. After the centrifuge cover 2 is closed, the drain pipe and suction pipe of the liquid suction device are matched with the suction pipe opening of the test tube. The liquid suction device 5 after the cover is closed rotates together with the centrifugal rotor 12. After centrifugation, it can extract the sample from the matched test tube. The motor 21 is fixed on the upper part of the bottom plate 22 in the casing 11. Its output shaft passes through the bottom of the centrifuge chamber 19 and is connected to the bottom of the centrifugal rotor 12, ensuring stable and reliable power transmission. During the centrifugation process, the shock-absorbing spring 20 and the flexible spring sleeve 23 in the casing 11 effectively absorb and reduce the vibration generated during the operation of the motor and the centrifugation process, reduce the damage of vibration to the internal parts of the equipment, and avoid the interference of vibration on the accuracy of the detection and extraction results.

[0065] During centrifugation, the heat dissipation plate 14 and its heat dissipation holes on the side of the chassis 11 away from the program interaction panel 3 will dissipate the heat generated by the operation of the equipment in a timely manner, maintain the internal temperature of the equipment within a reasonable range, and prevent equipment failure or sample quality from being affected by overheating.

[0066] After centrifugation, the liquid aspiration device 5 will automatically start according to the preset program to aspirate the supernatant and recover the waste liquid. When the liquid aspiration operation is completed and all experimental steps are finished, select the shutdown program on the program interaction panel 3 to turn off the centrifuge. After the centrifuge has completely stopped running, open the centrifuge lid 2. The centrifuge body 1 and the centrifuge lid 2 are matched by the lid locking mechanism 10 and the locking hook 15, and the opening and closing are controlled by the electric push rod 16. Then, the centrifuge tubes and the samples or products inside can be taken out.

[0067] In summary, this invention achieves an integrated process from cell to mitochondrial extraction through its equipped liquid aspiration device. The aspiration pump and waste liquid recovery pump, matched with the centrifuge tubes through a rational pipeline layout, can perform quantitative liquid aspiration and waste liquid recovery according to a preset program sequence. This design avoids the inconsistencies caused by human operation during multiple supernatant extractions, reduces the possibility of contamination from human operation, ensures the sterility and component stability of the extraction process, significantly improves the efficiency and success rate of mitochondrial extraction, and also reduces the difficulty and cost of clinical application. The platelet detection device located on the centrifuge lid detects platelet-related parameters such as quantity and volume using impedance spectroscopy. The centrifuge body and centrifuge lid are matched by a lid locking mechanism and hook, and the opening and closing are controlled by an electric push rod, ensuring the sealing and safety of the equipment during operation and preventing dangerous situations caused by accidental opening. The shock-absorbing springs and flexible spring sleeves installed inside the casing effectively reduce the damage to internal components caused by vibrations generated during motor operation and centrifugation, extending the service life of the equipment, and also reducing the impact of vibration on the accuracy of detection and extraction results, ensuring the stable operation of the centrifuge.

[0068] The above description is only a preferred embodiment of the present utility model and does not limit the implementation method and protection scope of the present utility model. Those skilled in the art should realize that all solutions obtained by equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A centrifuge adapted to clinical cell mitochondria extraction preparation centrifuge tube, characterized in that, include: The centrifuge body (1), the centrifuge cover (2) hinged to one side of the centrifuge body (1), the platelet detection device (4) fixedly disposed on the side of the centrifuge cover (2) away from the centrifuge body (1), and the liquid suction device (5) rotatably disposed on the side of the centrifuge cover (2) close to the centrifuge body (1); The centrifuge body (1) is fixedly provided with a cover locking mechanism (10) on the side near the centrifuge cover (2), and the centrifuge cover (2) is fixedly provided with a locking hook (15) on the side near the centrifuge body (1), and the locking hook (15) matches the cover locking mechanism (10). An electric push rod (16) is hinged in the centrifuge body (1), and the output end of the electric push rod (16) is hinged to the side of the centrifuge cover (2) near the centrifuge body (1). The liquid suction device (5) includes: a mounting bracket (51) fixedly disposed on one side near the centrifuge body (1), a plurality of suction pumps (52) fixedly disposed in the mounting bracket (51), and a waste liquid recovery pump (53) fixedly disposed in the mounting bracket (51). The bottom of the suction pump (52) is fixedly provided with a suction pipe (521) and a discharge pipe (522), and the bottom of the waste liquid recovery pump (53) is fixedly provided with a waste liquid recovery pipe (531) and a waste liquid discharge pipe (532). The waste liquid recovery pipe (531) is connected to the discharge pipe (522) of the suction pump (52) which is spatially adjacent to it; The suction tube (521) and the discharge tube (522) are matched with centrifuge tubes.

2. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 1, wherein, The centrifuge body (1) includes: a casing (11), a centrifuge rotor (12), a program interaction panel (3), a power socket (8), a motor (21), and a power switch (6); The centrifuge chamber (19) is fixedly arranged inside the chassis (11), the centrifuge rotor (12) is rotatably arranged in the centrifuge chamber (19), the program interaction panel (3) is fixedly arranged on one side of the chassis (11), the power socket (8) is fixedly arranged on the outside of the chassis (11), and the power switch (6) is fixedly arranged on one side of the chassis (11). The motor (21) is fixedly mounted on the upper part of the bottom plate (22) in the chassis (11), and the output shaft of the motor passes through the bottom of the centrifuge chamber (19) and is fixedly connected to the bottom of the centrifuge rotor (12). The centrifuge rotor (12) has several centrifuge tube slots (13) on its upper part, and the centrifuge tubes for the preparation of clinical cell mitochondria extraction are placed in the centrifuge tube slots (13).

3. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 2, characterized in that, The casing (14) on the side of the chassis (11) away from the program interaction panel (3) is a heat sink (14), and the heat sink (14) has several heat dissipation holes.

4. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 1, wherein, The platelet detection device (4) is fixedly provided with a platelet detection device interactive panel (7); The platelet detection device interactive panel (7) is electrically connected to the platelet detection device (4).

5. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 2, wherein, The aspiration pump (52), the waste liquid recovery pump (53), the platelet detection device (4), the program interaction panel (3), the cover locking mechanism (10), the electric push rod (16), the motor (21), the power switch (6), and the power socket (8) are all electrically connected.

6. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 1, wherein, Both the suction pump (52) and the waste liquid recovery pump (53) are diaphragm pumps; The outlet end of the suction pipe (521) is connected to the suction valve in the suction pump (52), and the inlet end of the discharge pipe (522) is connected to the discharge valve in the suction pump (52). The outlet end of the waste liquid recovery pipe (531) is connected to the suction valve in the waste liquid recovery pump (53), and the inlet end of the waste liquid discharge pipe (532) is connected to the discharge valve in the waste liquid recovery pump (53).

7. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 1, wherein, The mounting bracket (51) is cross-shaped, and the waste liquid recovery pump (53) is fixedly located at the center intersection of the mounting bracket (51). The mounting bracket (51) includes four branch rods, and the suction pump (52) is fixedly located on the four branch rods of the mounting bracket (51).

8. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 1, characterized in that, Also includes: The machine legs (9) are fixedly installed at the bottom of the centrifuge body (1); The machine legs (9) are located at the bottom of the centrifuge body (1) near the four corners.

9. The centrifuge adapted to the clinical cell mitochondrion extraction preparation centrifuge tube according to claim 2, wherein, The chassis (11) also includes: a shock-absorbing spring (20); one end of the shock-absorbing spring (20) is fixedly disposed on the upper part of the base plate (22), and the other end of the shock-absorbing spring (20) is fixedly disposed on the bottom of the centrifuge chamber (19); The shock-absorbing spring (20) is covered with a flexible spring sleeve (23).

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