A sequencing batch enrichment reactor for methanotrophic microorganisms

Abstract

The utility model relates to sewage treatment technical field, concretely to a kind of sequencing batch enrichment reaction device suitable for methane trophic microorganism, including reactor and mechanical stirring module installed in reactor, water bath circulation module, online data monitoring module, sample inlet exhaust port, sampling port, aeration module, bottom sludge discharge port, and the reaction device material is quartz glass;Compared with continuous flow and other methane trophic microorganism reactor operation mode, the sequencing batch reactor of the utility model can flexibly regulate and control aeration, sedimentation length, culture solution nutrient concentration and sludge concentration, conducive to the growth and reproduction of methane trophic microorganism;Compared with magnetic stirring heating or external heat preservation jacket and other heating mode, the utility model water bath circulation module temperature control is more stable, heat transfer is more uniform, further through online data monitoring module can strictly, effectively control operating condition.

Description

Technical Field

[0001] This utility model relates to the field of wastewater treatment technology, specifically to a sequencing batch enrichment reaction device suitable for methanogenic microorganisms. Background Technology

[0002] Since 2013, next-generation wastewater treatment concept plants based on energy self-sufficiency have been gradually engineered, simultaneously recovering energy during wastewater treatment and achieving ideal environmental, social, and economic benefits. Among these, the Adsorption-Bioprocess (AB process), with stage A primarily for carbon adsorption / capture and stage B primarily for biological denitrification, has become a highly promising wastewater treatment technology. However, stage A processes, mainly relying on chemically enhanced primary treatment, high-load activated sludge processes, or anaerobic digestion, can achieve dissolved methane concentrations in the effluent reaching 20 ppm. Using this as the organic carbon source for the stage B biological denitrification process can significantly reduce the operating costs of adding external carbon sources. Therefore, researching and developing a series of methane-enriching microbial systems lays the theoretical foundation for the large-scale application of the next-generation AB process.

[0003] International scholars confirmed the existence of anaerobic methane-oxidizing denitrifying bacteria and archaea in 2006 and 2013, respectively. However, these species are extremely sensitive to environmental factors (dissolved oxygen, light, temperature, etc.), with doubling times lasting several weeks, making them difficult to isolate and purify, thus hindering their development in wastewater treatment engineering. In recent years, scholars have successively discovered that aerobic methane-oxidizing bacteria under the Proteobacteria phylum can not only simultaneously oxidize and denitrify methane using methane as an electron donor and nitrate / nitrite as an electron acceptor under hypoxic conditions, but also use the organic metabolites produced by their methane oxidation as an ideal carbon source for traditional denitrifying bacteria, completing the methane-nutritional denitrification process in mixed bacterial systems. Currently, the enrichment culture of such methane-nutritional microorganisms is mostly concentrated in the laboratory pilot stage. To ensure enrichment effects and microbial activity, hollow fiber membrane aerated bioreactors are often selected, which are expensive, require continuous methane supply, and are inconvenient for the extraction of activated sludge samples at any time.

[0004] To overcome the existing drawbacks of methane oxidation-denitrification enrichment devices, improve microbial enrichment efficiency, reduce reactor preparation costs, and simplify experimental operation procedures, a high-efficiency enrichment device suitable for methane-nutritional microorganisms was designed and developed. This is of great significance for promoting the development and application of methane oxidation-denitrification autotrophic nitrogen removal processes with methane as the carbon source in "next-generation" wastewater treatment technologies. Utility Model Content

[0005] The purpose of this invention is to provide a sequencing batch enrichment reaction device suitable for methanogenic microorganisms, so as to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a sequencing batch enrichment reaction device suitable for methanogenic microorganisms, comprising a reactor and a mechanical stirring module, a water bath circulation module, an online data monitoring module, a sample inlet and outlet, a sampling port, an aeration module, and a bottom sludge discharge port installed in the reactor. The reaction device is made of quartz glass.

[0007] Preferably, the mechanical stirring module includes an electric motor and a mechanical stirring paddle; the electric motor can adjust the mechanical stirring speed, with a rotation speed in the range of 10-300 rpm.

[0008] Preferably, the water bath circulation module includes a circulation conduit, an external heating device connected to the circulation conduit, and a peristaltic pump.

[0009] Preferably, the online data monitoring module includes an integrated online monitoring probe for pH, temperature, and dissolved oxygen, and an online data display screen.

[0010] Preferably, the inlet and outlet are used for the inoculation of raw activated sludge, the inoculation of culture medium, and the discharge of gas during aeration. The inlet and outlet can also be used to add reagents when adjusting the pH, alkalinity and nutrient concentration of the reactor.

[0011] Preferably, there are multiple sampling ports, which are distributed from the middle to the bottom of the reactor. Three sampling ports are set at equal intervals on both sides of the reactor. The sampling ports on the same side of the reactor are set from top to bottom as the upper port, the middle port and the lower port.

[0012] Preferably, the aeration module includes an aeration conduit, a sand core aeration head connecting conduit, and a sand core aeration component.

[0013] Preferably, the sand core aeration assembly is located at the bottom of the reactor and connected via an aeration head connecting conduit.

[0014] Preferably, the aeration conduit is connected to the sample inlet and outlet via a silicone tube, and the gas in the reactor is pumped out from the sample inlet and outlet via a peristaltic pump and pumped into the aeration conduit.

[0015] Preferably, the sludge discharge port is located at the bottom of the reactor and is equipped with a rotary control switch.

[0016] Compared with the prior art, the beneficial effects of this utility model are: (1) Compared with the operation mode of continuous flow and other methane-nutritional microbial reactors, the sequencing batch reactor of this invention can flexibly control the aeration and sedimentation time, the concentration of nutrients in the culture medium and the sludge concentration, which is conducive to the growth and reproduction of methane-nutritional microorganisms.

[0017] (2) Compared with heating methods such as magnetic stirring heating or external insulation layer, the water bath circulation module of this utility model has more stable temperature control and more uniform heat transfer. Furthermore, the online data monitoring module can strictly and effectively control the operating conditions.

[0018] (3) Compared with aeration methods such as hollow fiber membrane aeration, the four sand core aeration heads in the aeration module of this utility model can effectively increase the contact area between methane gas and sludge mixture, and improve the methane mass transfer efficiency; the two gases are introduced at the same time through the aeration pipes on both sides, which improves the reactor operating efficiency; the aeration pipes are connected to the inlet (exhaust port) to realize the recycling of methane gas and save the cost of methane-enriched microorganisms. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of a methane-nutritive microbial sequencing batch enrichment reactor. Figure 2 This is a schematic diagram of the bottom sand core aeration component of a methane-nutritive microbial sequencing batch enrichment reactor.

[0020] In the diagram: 1. Electric motor; 2. Water bath circulation conduit; 3. Online data display screen; 4. Online probe insertion port; 5. Sample inlet and outlet port; 6. Integrated online monitoring probe for pH, temperature, and dissolved oxygen; 7. Mechanical agitator; 8. Sampling port; 9. Aeration conduit; 10. Bottom sludge discharge port; 11. Sand core aeration head connecting conduit; 12. Sand core aeration assembly. Detailed Implementation

[0021] The technical solutions in the embodiments of this utility model will be clearly and completely described below. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0022] Please see Figures 1 to 2 This utility model provides a technical solution: a sequencing batch enrichment reaction device suitable for methanogenic microorganisms, including a reactor and a mechanical stirring module, a water bath circulation module, an online data monitoring module, a sample inlet and exhaust port 5, a sampling port 8, an aeration module, and a bottom sludge discharge port 10 installed in the reactor. The reaction device is made of quartz glass.

[0023] The mechanical stirring module includes an electric motor 1 and a mechanical stirring paddle 7. The electric motor 1 can adjust the mechanical stirring speed, with a rotation speed in the range of 10-300 rpm. The type of the mechanical stirring paddle 7 can be selected or replaced according to the type of enriched activated sludge, such as a two-blade paddle, a three-blade paddle, or a spiral paddle. The mechanical stirring module is used to mix and stir the activated sludge and culture liquid wastewater in the reactor, ensuring that the sludge and water are mixed evenly without damaging or breaking up the sludge flocs.

[0024] The water bath circulation module includes a circulation conduit 2, an external heating device and a peristaltic pump connected to the circulation conduit 2; the external heating device can set the water bath temperature according to the requirements of microbial enrichment culture, ranging from 4℃ to 60℃, and the peristaltic pump circulates the water bath through the conduit to ensure a constant temperature inside the reactor.

[0025] The online data monitoring module includes an integrated online monitoring probe 6 for pH, temperature, and dissolved oxygen, and an online data display screen 3. The integrated probe can monitor the real-time pH, temperature, and dissolved oxygen in the reactor. If any abnormality is detected, it can guide the adjustment of reactor operating parameters or restore normal operating parameters through chemical dosing or aeration.

[0026] The inlet and outlet 5 is used for the inoculation of raw activated sludge, the inoculation of culture medium wastewater, and the discharge of gas during aeration. The inlet and outlet 5 can also be used to add reagents when adjusting the pH, alkalinity and nutrient concentration of the reactor. The steps of inlet, aeration, venting, sedimentation and effluent discharge of this sequencing batch reactor are carried out alternately during the enrichment process. When the inlet and outlet 5 is not used, it must be strictly sealed with a silicone sealing plug.

[0027] Multiple sampling ports 8 are provided, and the multiple sampling ports 8 are distributed from the middle to the bottom of the reactor. Three sampling ports 8 are set at equal intervals on both sides of the reactor. The sampling ports 8 on the same side of the reactor are set as the upper pipe port, the middle pipe port and the lower pipe port from top to bottom, which facilitates the acquisition of water samples or sludge samples at each reaction stage.

[0028] The aeration module includes an aeration conduit 9, a sand core aeration head connecting conduit 11, and a sand core aeration component 12. By introducing inert gas into the reactor through the aeration module, the reactor can be kept in an anoxic or anaerobic state. Introducing methane gas into the reactor can keep the reactor with a sufficient carbon source. The sand core aeration component can increase the contact area between the gas and the culture liquid in the reactor, and accelerate the utilization efficiency of methane by the activated sludge.

[0029] The sand core aeration component 12 is located at the bottom of the reactor and is connected to the sand core aeration head connecting pipe 11. The bottom pipe is equipped with four sets of sand core aeration heads, and air can be introduced at both ends 9, so as to realize the simultaneous aeration of two gases and improve the working efficiency of the reactor.

[0030] The aeration conduit 9 is connected to the sample inlet and exhaust outlet 5 via a silicone tube. A peristaltic pump pumps the gas in the reactor out of the sample inlet and exhaust outlet 5 and into the aeration conduit 9 to achieve the recycling of methane gas.

[0031] The sludge discharge port 10 is located at the bottom of the reactor and is equipped with a knob control switch for discharging excess sludge or mud-water mixture.

[0032] The working method includes the following steps: S1. Close the upper, middle and lower sampling ports 8, aeration pipe 9, sand core aeration head connecting pipe 11 and bottom sludge discharge port 10. Pour about 1 / 3 of the reactor volume of activated sludge into the sampling port 5 of the reaction device, and add culture medium to 2 / 3 to 3 / 4 of the reactor volume to complete the sludge inoculation process. S2. Open the mechanical stirring paddle 7, the water bath circulation module including the water bath circulation pipe 2, the integrated online monitoring probe 6 and the online data display screen 3 to ensure that the activated sludge is stirred at a constant temperature and at a constant speed, and monitor the pH value, temperature and dissolved oxygen concentration of the activated sludge in the reactor in real time through the online monitoring probe and the digital display screen. S3. Open the exhaust port 5 and connect the methane and inert gas to the aeration pipes 9 on both sides of the bottom of the reaction device. According to the actual process requirements, the two gases are simultaneously introduced into the reaction device through the sand core aeration head connecting pipe 11 and sand core aeration component 12, or the two gases are introduced in sequence, so as to reduce dissolved oxygen and provide sufficient methane carbon source. S4. During the single-cycle culture process, the aeration conduit 9 can be connected to the sample inlet and exhaust outlet 5 through a silicone tube. The gas in the reactor is pumped out from the sample inlet and exhaust outlet and pumped in through the aeration conduit by a peristaltic pump to realize the recycling of methane gas. S5. During the single-cycle cultivation process, based on the data displayed by the integrated online monitoring probe 6 and the online data display screen 3, the temperature inside the reactor can be adjusted by the water bath circulation module, the pH value of the activated sludge can be adjusted by injecting chemical reagents into the reactor through the inlet 5, and the dissolved oxygen concentration can be adjusted by aerating the reaction device through the sand core aeration head connecting pipe 11 and sand core aeration component 12. S6. Turn off the agitator 7, let it settle for a certain period of time, and take water samples from the upper, middle and lower sampling ports 8 according to the experimental requirements. Test the relevant water quality indicators. Similarly, discharge the remaining culture medium through the upper, middle and lower sampling ports 8. If there is any remaining sludge, discharge it from the bottom sludge discharge port 10. S7. During mechanical stirring, mud-water mixture samples can be obtained from the upper, middle, and lower sampling ports 8 at any time for microbial DNA extraction, which facilitates subsequent analysis of the species composition and metabolic function of microorganisms in the reactor under any conditions.

[0033] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sequencing batch enrichment reactor suitable for methanogenic microorganisms, characterized in that: The reactor includes a mechanical stirring module, a water bath circulation module, an online data monitoring module, a sample inlet and outlet (5), a sampling port (8), an aeration module, and a bottom sludge discharge port (10). The reaction device is made of quartz glass.

2. The sequencing batch enrichment device for methanogenic microorganisms according to claim 1, characterized in that: The mechanical stirring module includes an electric motor (1) and a mechanical stirring paddle (7); the electric motor (1) can adjust the mechanical stirring speed, with a rotation speed in the range of 10-300 rpm.

3. The sequencing batch enrichment device for methanogenic microorganisms according to claim 1, characterized in that: The water bath circulation module includes a circulation conduit (2), an external heating device for the circulation conduit (2), and a peristaltic pump.

4. The sequencing batch enrichment device for methanogenic microorganisms according to claim 1, characterized in that: The online data monitoring module includes an integrated online monitoring probe (6) for pH, temperature, and dissolved oxygen, and an online data display screen (3).

5. The sequencing batch enrichment device for methanogenic microorganisms according to claim 1, characterized in that: The inlet and outlet (5) is used for the inoculation of raw activated sludge, the inoculation of culture medium, and the discharge of gas during aeration. The inlet and outlet (5) can also be used to add agents when adjusting the pH, alkalinity and nutrient concentration of the reactor.

6. The sequencing batch enrichment device for methanogenic microorganisms according to claim 1, characterized in that: There are multiple sampling ports (8), and the multiple sampling ports (8) are distributed from the middle to the bottom of the reactor. Three sampling ports (8) are set at equal intervals on both sides of the reactor. The sampling ports (8) on the same side of the reactor are set as the upper pipe port, the middle pipe port and the lower pipe port from top to bottom.

7. A sequencing batch enrichment reaction apparatus for methanogenic microorganisms according to any one of claims 1 to 6, characterized in that: The aeration module includes an aeration conduit (9), a sand core aeration head connecting conduit (11), and a sand core aeration component (12).

8. A sequencing batch enrichment reaction device for methanogenic microorganisms according to claim 7, characterized in that: The sand core aeration assembly (12) is located at the bottom of the reactor and is connected via a sand core aeration head connecting conduit (11).

9. A sequencing batch enrichment reaction device for methanogenic microorganisms according to claim 8, characterized in that: The aeration conduit (9) is connected to the sample inlet and outlet (5) via a silicone tube. The gas in the reactor is pumped out from the sample inlet and outlet (5) and pumped into the aeration conduit (9) by a peristaltic pump.

10. A sequencing batch enrichment reaction device for methanogenic microorganisms according to claim 1, characterized in that: The sludge discharge port (10) is located at the bottom of the reactor and is equipped with a knob control switch.

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