A water total bacteria detection device based on single particle analysis method

Abstract

The utility model belongs to water body bacterial microorganism total number detection technical field especially for a kind of water total bacteria detection device based on single particle analysis method, including work plate, the periphery of the upper side of work plate is equipped with first support column, the upper side of first support column is equipped with mixing box, the upper side of mixing box is equipped with filter plate, the upper side of filter plate is equipped with filter box, one side of filter box is equipped with second water pipe, the surface of second water pipe is equipped with second water pump, the upper side of mixing box is equipped with dyeing box, by mixing box, filter box, dyeing box, motor, flow chamber, conical flow chamber, detection box, collection box, support column, support base and so on mutually cooperate, so that the device directly, quickly count every one bacterial individual (or particle) in water sample, to obtain total bacteria concentration, without lengthy culture process, satisfy the high throughput, fast, non-culture dependent detection technology of water body.

Description

Technical Field

[0001] This invention belongs to the field of total bacterial count detection technology in water bodies, specifically relating to a device for detecting total bacteria in water bodies based on a single-particle analysis method. Background Technology

[0002] A water body is a collection of water, encompassing rivers, lakes, seas, groundwater, glaciers, etc. It is a natural complex of areas covered by water. It includes not only water but also dissolved substances, suspended solids, sediment, and aquatic organisms. It is an important component of the surface hydrosphere, a natural body of water bounded by relatively stable land, including rivers, lakes, seas, glaciers, snow cover, reservoirs, ponds, groundwater, and atmospheric water vapor. Surface water is closely related to people's lives and production activities. It can be classified according to different uses, such as agricultural irrigation water, fishery water, and drinking water. Different water quality requirements exist, and corresponding water quality standards have been established as the basis for water quality control. With increasingly serious drinking water safety issues and stricter testing standards, traditional detection methods can no longer meet current monitoring needs. A high-throughput, rapid, culture-independent detection technology is currently needed. Therefore, a total bacterial count detection device for water bodies based on a single-particle analysis method is designed. Utility Model Content

[0003] To address the problems mentioned in the background art, this utility model provides a water total bacteria detection device based on a single-particle analysis method, which solves the problem of high-throughput and rapid detection of water total bacteria.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a water total bacteria detection device based on single-particle analysis, comprising a working plate, first support columns installed around the upper side of the working plate, a mixing chamber installed on the upper side of the first support columns, a filter plate installed on the upper side of the mixing chamber, a filter chamber installed on the upper side of the filter plate, a second water pipe installed on one side of the filter chamber, a second water pump installed on the surface of the second water pipe, a staining chamber installed on the upper side of the mixing chamber, a third water pipe installed on one side of the staining chamber, a third water pump installed on the surface of the third water pipe, and a throttling valve installed on the lower side of the mixing chamber. A sample tube is installed on the other side of the throttling valve. A support frame is installed on the lower side of the mixing box. A flow chamber is installed at the other end of the support frame. A first water pipe is installed on one side of the flow chamber. A first water pump is installed on the surface of the first water pipe. A conical flow chamber is installed on the lower side of the flow chamber. A detection box is installed on the lower side of the conical flow chamber. A laser is installed on one side of the detection box. A second detector is installed on the other side of the detection box. A first detector is installed on one side of the detection box. A collection box is installed on the lower side of the detection box. A fourth water pipe is installed on one side of the collection box. A fourth water pump is installed on the surface of the fourth water pipe.

[0005] Preferably, a second support column is installed around the lower side of the working plate, and a support base is installed on the lower side of the second support column.

[0006] Preferably, the detection box and the collection box are connected by an opening, and the detection box and the collection box are fully sealed dark boxes.

[0007] Preferably, the inner sides of the flow chamber and the cone flow chamber are filled with sheath fluid, and the sheath fluid is located around the sample tube.

[0008] Preferably, there are two support frames, and the support frames are located on both sides of the flow chamber.

[0009] Preferably, the sample tube and the laser are in the same plane.

[0010] Preferably, a motor is installed on the upper side of the mixing box, a rotating shaft is installed at the output end of the motor, a rotating block is installed on the other side of the rotating shaft, and impellers are installed around the rotating block.

[0011] Compared with the prior art, the beneficial effects of this utility model are:

[0012] By coordinating various components such as the mixing chamber, filtration chamber, staining chamber, motor, flow chamber, conical flow chamber, detection chamber, collection chamber, support column, and support base, this device can directly and quickly count every individual bacteria (or particle) in a water sample to obtain the total bacterial concentration without a lengthy culture process, thus meeting the requirements for high-throughput, rapid, and culture-independent detection technology for water bodies. Attached Figure Description

[0013] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0014] Figure 1 This is a first three-dimensional structural diagram of the present invention;

[0015] Figure 2 This is a cross-sectional view of the present invention;

[0016] Figure 3 This is a second three-dimensional structural diagram of the present invention.

[0017] In the diagram: 1. Working plate; 2. First support column; 3. Mixing box; 4. Filter box; 5. Staining box; 6. Motor; 7. Flow chamber; 8. Conical flow chamber; 9. Detection box; 10. Collection box; 11. Second support column; 12. Support base; 13. First water pump; 14. First water pipe; 15. Rotating shaft; 16. Rotating block; 17. Impeller; 18. Filter plate; 19. Throttling valve; 20. Support frame; 21. Sample tube; 22. Laser; 23. First detector; 24. Second detector; 25. Second water pump; 26. Second water pipe; 27. Third water pump; 28. Fourth water pump; 29. ​​Fourth water pipe; 30. Third water pipe. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0019] Please see Figure 1-3This utility model provides the following technical solution: A water total bacteria detection device based on single-particle analysis method, comprising a working plate 1, first support columns 2 installed around the upper side of the working plate 1, a mixing tank 3 installed on the upper side of the first support columns 2, a filter plate 18 installed on the upper side of the mixing tank 3, a filter box 4 installed on the upper side of the filter plate 18, a second water pipe 26 installed on one side of the filter box 4, a second water pump 25 installed on the surface of the second water pipe 26, a staining tank 5 installed on the upper side of the mixing tank 3, a third water pipe 30 installed on one side of the staining tank 5, a third water pump 27 installed on the surface of the third water pipe 30, and a throttling valve 19 installed on the lower side of the mixing tank 3. A sample tube 21 is installed on one side, a support frame 20 is installed on the lower side of the mixing box 3, a flow chamber 7 is installed on the other end of the support frame 20, a first water pipe 14 is installed on one side of the flow chamber 7, a first water pump 13 is installed on the surface of the first water pipe 14, a conical flow chamber 8 is installed on the lower side of the flow chamber 7, a detection box 9 is installed on the lower side of the conical flow chamber 8, a laser 22 is installed on one side of the detection box 9, a second detector 24 is installed on the other side of the detection box 9, a first detector 23 is installed on one side of the detection box 9, a collection box 10 is installed on the lower side of the detection box 9, a fourth water pipe 29 is installed on one side of the collection box 10, and a fourth water pump 28 is installed on the surface of the fourth water pipe 29.

[0020] In this embodiment, by setting the sample tube 21 and the laser 22 to be on the same horizontal plane, the laser 22 can accurately irradiate the sample, thereby increasing the accuracy of the detection.

[0021] In this embodiment, impurities in the water can be removed by setting up a filter plate 18.

[0022] In this embodiment, by setting the throttle valve 19, the flow rate of the sample can be controlled, and the sample flow rate can be precisely controlled.

[0023] In this embodiment, by configuring the motor 6, rotating shaft 15, rotating block 16, and impeller 17, the staining agent and sample in the mixing chamber 3 are thoroughly mixed.

[0024] The working principle and usage process of this utility model are as follows: After installation, the water sample to be tested is poured into the filter box 4 through the water pipe connected to the second water pipe 26, driven by the second water pump 25. The water in the filter box 4 is filtered above the filter plate 18 and then flows into the mixing box 3. The dye is poured into the staining box 5 through the water pipe connected to the third water pipe 30, driven by the third water pump 27. The dye falls from the staining box 5 into the mixing box 3. By turning on the motor 6, the rotating shaft 15 of the motor 6 is rotated, thereby rotating the rotating block 16, which in turn rotates the impeller 17, thus accelerating the fusion of the dye and the water sample in the mixing box 3. After thorough agitation, the motor 6 is turned off. The pipe connected to the first water pipe 14 is driven by the first water pump 13 to fill the flow chamber 7 with sheath fluid. The throttle valve 19 is opened, allowing the water sample to flow into the sample tube 21. The water sample is wrapped and compressed by the sheath fluid, so that it flows through in a single stream and finally falls into the collection box 10. When the water sample falls into the detection box 9, the laser 22 is turned on to irradiate the water sample to excite bacterial fluorescence. Then the first detector 23 and the second detector 24 can capture the characteristic information of the bacteria. After the detection is completed, the water in the collection box 10 can be extracted through the fourth water pipe 29. All electrical equipment in this device is powered by an external power supply, and all motors in this device are connected and controlled by a PLC control system.

[0025] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A device for detecting total bacteria in water based on a single-particle analysis method, comprising a working plate (1), characterized in that: The upper side of the working plate (1) is equipped with a first support column (2) around its perimeter. A mixing box (3) is installed on the upper side of the first support column (2). A filter plate (18) is installed on the upper side of the mixing box (3). A filter box (4) is installed on the upper side of the filter plate (18). A second water pipe (26) is installed on one side of the filter box (4). A second water pump (25) is installed on the surface of the second water pipe (26). A dyeing box (5) is installed on the upper side of the mixing box (3). A third water pipe (30) is installed on one side of the dyeing box (5). A third water pump (27) is installed on the surface of the third water pipe (30). A throttle valve (19) is installed on the lower side of the mixing box (3). A sample tube (21) is installed on the other side of the throttle valve (19). A support frame (20) is provided, and a flow chamber (7) is installed at the other end of the support frame (20). A first water pipe (14) is installed on one side of the flow chamber (7), and a first water pump (13) is installed on the surface of the first water pipe (14). A conical flow chamber (8) is installed on the lower side of the flow chamber (7), and a detection box (9) is installed on the lower side of the conical flow chamber (8). A laser (22) is installed on one side of the detection box (9), and a second detector (24) is installed on the other side of the detection box (9). A first detector (23) is installed on one side of the detection box (9), and a collection box (10) is installed on the lower side of the detection box (9). A fourth water pipe (29) is installed on one side of the collection box (10), and a fourth water pump (28) is installed on the surface of the fourth water pipe (29).

2. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: A second support column (11) is installed around the lower side of the working plate (1), and a support base (12) is installed on the lower side of the second support column (11).

3. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: The detection box (9) and the collection box (10) are connected by an opening, and the detection box (9) and the collection box (10) are fully sealed dark boxes.

4. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: The inner sides of the flow chamber (7) and the conical flow chamber (8) are filled with sheath fluid, and the sheath fluid is located around the sample tube (21).

5. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: The number of support frames (20) is two, and the support frames (20) are located on both sides of the flow chamber (7).

6. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: The sample tube (21) and the laser (22) are in the same plane.

7. The water total bacteria detection device based on single-particle analysis method according to claim 1, characterized in that: A motor (6) is installed on the upper side of the mixing box (3), and a rotating shaft (15) is installed at the output end of the motor (6). A rotating block (16) is installed on the other side of the rotating shaft (15), and an impeller (17) is installed around the rotating block (16).

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