Exosome concentration preparation device

Abstract

The utility model discloses a kind of exosome concentration preparation devices, comprising: cover, exosome tank, connector, concentration tank and regulator, the cover, exosome tank, connector, concentration tank and regulator are sequentially arranged from top to bottom, the upper and lower ends of the exosome tank and concentration tank are respectively provided with first thread groove and second thread groove, the inside middle part and inside lower of the connector are respectively provided with annular clamping groove and step, the top of the step is equipped with dialysis membrane, and the top of the dialysis membrane is equipped with grid support. The utility model can adjust the addition amount of concentrated agent by adjusting the height position of piston, ensure that concentrated agent and dialysis membrane are in close contact, can also extrude water-absorbing sponge type concentrated agent to discharge moisture, restore its water absorption performance, improve the rate of reuse, and the device is connected by thread, and the components are assembled and disassembled conveniently, suitable for laboratory rapid processing large volume sample, without relying on complex equipment such as ultracentrifugation.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, and in particular to an exosome concentration and extraction device. Background Technology

[0002] Exosomes are small vesicles (30-150 nm) released from cells via multivesicular bodies (MBVs). They exist both inside and outside cellular organisms. In the human body, they are widely distributed, found in bodily fluids such as blood, sweat, breast milk, and urine. Exosomes function primarily through intercellular communication, transmitting information from donor cells to recipient cells to perform functions such as immune regulation, antigen presentation, and substance transport.

[0003] However, common exosome samples are relatively large, while existing separation and purification methods, such as ultracentrifugation and size exclusion chromatography, process smaller samples. Therefore, concentration is required before separation and purification to reduce the sample volume. Existing exosome concentration methods mainly rely on ultracentrifugation, filtration, and other techniques. These methods require expensive equipment, are complex to operate, and have low efficiency. In addition, existing concentration devices often have problems such as difficulty in replacing dialysis membranes and low reusability of concentrates, which limit their application scope. Utility Model Content

[0004] One objective of this invention is to provide an exosome concentration and extraction device. This invention addresses the problems mentioned in the background above, where existing exosome concentration methods mainly rely on technologies such as ultracentrifugation and filtration. These methods require expensive equipment, are complex to operate, and have low efficiency. In addition, existing concentration devices often suffer from problems such as difficulty in replacing dialysis membranes and low reusability of concentrates, which limit their application scope.

[0005] An exosome concentration and extraction apparatus according to an embodiment of the present invention includes: The device comprises a cap, an exosome container, a connector, a concentration container, and a regulator, arranged sequentially from top to bottom. The exosome container and the concentration container have a first threaded groove and a second threaded groove at their upper and lower ends, respectively. The connector has an annular groove and a step at its inner center and lower inner side, respectively. A dialysis membrane is provided at the top of the step, and a grid support is provided at the top of the dialysis membrane. An elastic card is provided on the outer side of the grid support. The concentration container is connected to the regulator via an adjustable component to adjust the amount of concentrate added.

[0006] Preferably, the cover is threadedly fixed to the exosome tank via a first threaded groove, the connector is threadedly fixed to the concentration tank via a second threaded groove, the exosome tank is threadedly fixed to the connector via a first threaded groove, and the concentration tank is threadedly fixed to the connector via a second threaded groove.

[0007] Preferably, sealing gaskets are installed at both the upper and lower ends of the inner side of the connector, with the upper sealing gasket located between the exosome tank and the connector, and the lower sealing gasket located between the concentration tank and the connector.

[0008] Preferably, several elastic cards are arranged in a circular shape, and the grid bracket and elastic cards are engaged with the connector through an annular slot.

[0009] Preferably, the exosome container is used to store the exosome solution to be concentrated or the sample to be dialyzed, and the inside of the concentration container is filled with a concentrator.

[0010] Preferably, the adjustable component includes a guide post fixed to the top of the fixture and a knob that rotates at the bottom of the fixture. A limiting groove is formed inside the guide post, and a telescopic post is movably arranged inside the guide post through the limiting groove. A piston is fixedly arranged at the top of the telescopic post, and a screw is fixedly arranged at the top of the knob. The screw and the telescopic post are connected by a threaded transmission.

[0011] Preferably, the piston is adapted to the inner diameter of the concentration tank, the piston is located inside the concentration tank and is movable, and a limit plate is installed on the top of the guide column.

[0012] The beneficial effects of this utility model are: 1. This utility model, through the design of an exosome concentration and extraction device, effectively avoids the problems of traditional concentration methods relying on expensive equipment and complex operation. During use, the dialysis membrane in the connector is installed by engaging with an elastic card and an annular groove. When contaminants adhere to the membrane surface, the dialysis membrane can be quickly replaced by pushing the grid support upward, causing the elastic card to disengage from the annular groove, thus preventing a decrease in concentration efficiency. Furthermore, the screw is rotated by turning the knob of the adjustable component. Since the screw and the telescopic column are driven by a threaded transmission, the telescopic column is driven to move vertically and stably along the limiting groove of the guide column, thereby driving the piston at the top to move precisely within the concentration tank. By adjusting the height of the piston, the amount of concentrate added can be adjusted to ensure close contact between the concentrate and the dialysis membrane. At the same time, it can squeeze out water from the absorbent sponge-like concentrate, restoring its absorbency and improving the reusability. Moreover, the components of this device are connected by threads, making assembly and disassembly convenient. It is suitable for rapid processing of large-volume samples in the laboratory without relying on complex equipment such as ultracentrifuges. 2. This utility model, through the design of the dialysis membrane and sealing gaskets, allows the membrane to be replaced as an independent consumable, avoiding the scrapping of the entire device and significantly reducing long-term operating costs. When the cap, exosome container, connector, and concentration container are assembled via threaded connections, the sealing gaskets installed at the upper and lower ends of the connector are compressed, forming a seal between the exosome container and the connector at the top and a seal between the concentration container and the connector at the bottom. This effectively avoids potential liquid leakage or gas infiltration at the connection points of various components, ensuring the airtightness of the chamber throughout the entire concentration dialysis process, preventing sample contamination or reagent loss, and guaranteeing the stability of the concentration process and the reliability of experimental results. Attached Figure Description

[0013] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0014] Figure 1 This is a three-dimensional structural diagram of one side of an exosome concentration and extraction device proposed in this utility model; Figure 2 This is a schematic diagram of the explosion structure of an exosome concentration and extraction device proposed in this utility model; Figure 3 This is a schematic diagram of the internal structure of the connector of the exosome concentration and extraction device proposed in this utility model; Figure 4 This is a schematic diagram of the grid support structure of an exosome concentration and extraction device proposed in this utility model; Figure 5 This is a schematic diagram of the piston structure of an exosome concentration and extraction device proposed in this utility model; In the diagram: 1. Cap; 2. Exosome container; 3. Connector; 4. Concentrator; 5. Regulator; 6. First threaded groove; 7. Second threaded groove; 8. Sealing gasket; 9. Annular groove; 10. Step; 11. Dialysis membrane; 12. Grid support; 13. Elastic clip; 14. Guide post; 15. Limiting groove; 16. Telescopic post; 17. Limiting plate; 18. Piston; 19. Screw; 20. Knob. Detailed Implementation

[0015] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, illustrating only the basic structure of the present invention, and therefore only show the components relevant to the present invention.

[0016] refer to Figures 1-4 An exosome concentration and extraction apparatus, comprising: The system comprises a cover 1, an exosome container 2, a connector 3, a concentration container 4, and a regulator 5, arranged sequentially from top to bottom. The exosome container 2 and the concentration container 4 have a first threaded groove 6 and a second threaded groove 7 at their top and bottom ends, respectively. The connector 3 has an annular groove 9 and a step 10 on its inner middle and lower sides, respectively. A dialysis membrane 11 is mounted on the top of the step 10, and a mesh support 12 is mounted on the top of the dialysis membrane 11. The connector 3 contains a replaceable dialysis membrane 11, which can be easily replaced by the user. 11. Cleaning or replacing the membrane is necessary to prevent excessive contaminants from adhering to the membrane surface, which would reduce concentration efficiency and improve practicality. The outer side of the grid support 12 is equipped with an elastic card 13. The concentration tank 4 is connected to the regulator 5 through an adjustable component to adjust the amount of concentrate added. Adjusting the amount of concentrate added ensures that the concentrate is in close contact with the dialysis membrane 11, ensuring a good concentration effect. It can also be used as a dehydration lever for absorbent sponges and other concentrates to squeeze out the absorbed water, restore the water absorption performance of the concentrate, improve the reusability of the concentrate, and reduce concentration costs. The adjustable component includes a guide post 14 fixed to the top of the fixture and a knob 20 that rotates at the bottom of the fixture. A limiting groove 15 is provided inside the guide post 14. A telescopic post 16 is movably arranged inside the guide post 14 through the limiting groove 15. A piston 18 is fixedly arranged on the top of the telescopic post 16. A screw 19 is fixedly arranged on the top of the knob 20. The screw 19 and the telescopic post 16 are connected by a threaded transmission. Rotating the knob 20 of the adjustable component drives the screw 19 to rotate. Since the screw 19 and the telescopic column 16 are driven by a threaded transmission, the telescopic column 16 is driven to move vertically and stably along the limiting groove 15 of the guide column 14. This, in turn, drives the piston 18 at the top to move precisely within the concentration tank 4. By adjusting the height of the piston 18, the amount of concentrate added can be adjusted to ensure that the concentrate is in close contact with the dialysis membrane 11. At the same time, it can squeeze the absorbent sponge-like concentrate to remove water, restore its water absorption performance, and improve the reuse rate.

[0017] Example 1: The cover 1 is threadedly fixed to the exosome tank 2 via the first threaded groove 6. The cover 1 is detachable, which facilitates feeding and maintaining air pressure balance. The connector 3 is threadedly fixed to the concentration tank 4 via the second threaded groove 7. The exosome tank 2 is threadedly fixed to the connector 3 via the first threaded groove 6. The concentration tank 4 is threadedly fixed to the connector 3 via the second threaded groove 7. All components of this device are connected by threads, making assembly and disassembly convenient. It is suitable for rapid processing of large-volume samples in the laboratory without relying on complex equipment such as ultracentrifuges.

[0018] Example 2: Sealing gaskets 8 are installed at both the upper and lower ends of the inner side of connector 3. The upper sealing gasket 8 is located between exosome tank 2 and connector 3, and the lower sealing gasket 8 is located between concentration tank 4 and connector 3. When the cap 1, exosome container 2, connector 3 and concentration container 4 are assembled by threaded connection, the sealing gaskets 8 installed at the upper and lower ends of connector 3 are compressed, forming a seal between exosome container 2 and connector 3 at the top and a seal between concentration container 4 and connector 3 at the bottom. This effectively avoids liquid leakage or gas infiltration problems that may occur at the connection of each component, ensures the airtightness of the chamber during the entire concentration dialysis process, prevents sample contamination or reagent loss, and guarantees the stability of the concentration process and the reliability of experimental results.

[0019] Example 3: Several elastic cards 13 are arranged in a circle. The grid support 12 and the elastic cards 13 are installed in conjunction with the connector 3 through the annular slot 9. The dialysis membrane 11 in the connector 3 is installed in conjunction with the elastic cards 13 and the annular slot 9. When contaminants adhere to the membrane surface, the dialysis membrane 11 can be quickly replaced by pushing the grid support 12 upward to disengage the elastic cards 13 from the annular slot 9, thus avoiding a decrease in concentration efficiency. The exosome container 2 is used to store the exosome solution to be concentrated or the sample to be dialyzed. The concentration container 4 is filled with a concentrate, which is a porous material that can absorb a large amount of water, such as an absorbent sponge or PEG.

[0020] Example 4: The piston 18 is adapted to the inner diameter of the concentration tank 4. The piston 18 is located inside the concentration tank 4 and can be moved. By adjusting the height of the piston 18, the amount of concentrate added can be adjusted to ensure that the concentrate is in close contact with the dialysis membrane 11. At the same time, it can squeeze the water-absorbing sponge-like concentrate to discharge water and restore its water absorption performance. A limit plate 17 is installed on the top of the guide column 14 to limit the distance of movement of the telescopic column 16.

[0021] Working principle: Before use, the overall condition of the device must be checked to ensure the airtightness of the dialysis membrane 11 and the entire device. The device consists of a cover 1, an exosome container 2, a connector 3, a concentration tank 4, and a regulator 5. All components are connected by threads. The cover 1 is used to add exosome-containing solution and maintain pressure balance. The exosome container 2 is used to store the sample to be concentrated. When assembling the cover 1, exosome container 2, connector 3, and concentration tank 4 through threaded connections, the sealing gaskets 8 installed at the upper and lower ends of the connector 3 are compressed, forming a gap between the exosome container 2 and the connector 3 at the top. A seal is formed between the concentration tank 4 and the connector 3 below. The connector 3 contains a detachable mesh support 12 and a dialysis membrane 11. The dialysis membrane 11 selectively allows small molecules such as water molecules to pass through, and is used with a concentrate to concentrate exosomes. The mesh support 12 provides support. The concentration tank 4 is used to store and replace the concentrate or dialysis fluid. The concentrate is a porous material that can absorb a large amount of water (such as absorbent sponge, PEG, etc.). The regulator 5 installed at its bottom rotates the screw 19 by turning the knob 20, and uses the threaded transmission to drive the telescopic column 16 vertically along the guide column 14. The piston 18 moves precisely within the concentration tank 4 by vertically raising and lowering itself. Adjusting the height of the piston 18 controls the amount of concentrate added, ensuring close contact between the concentrate and the dialysis membrane 11. It can also act as a dehydration lever for absorbent sponge-type concentrates, squeezing out absorbed water to restore absorbency, thus improving the concentrate's reusability and reducing costs. During operation, first, load the concentrate or dialysis fluid into the concentration tank 4, screw on the connector 3 and insert the dialysis membrane 11. Then, screw on the exosome container 2 and add the sample. Finally, tighten the cap 1 and adjust the regulator 5 upwards to ensure close contact between the concentrate and the dialysis membrane 11. With close contact, place the assembled device on a horizontal shaker to complete the concentration or dialysis process. When the dialysis membrane 11 is adhered to by contaminants, the device can be rotated 180°, the connector 3 can be unscrewed from the exosome tank 2, the remaining components can be rotated and the dialysis membrane 11 replaced, and then rotated back to the original state and connected to the exosome tank 2 to continue operation. If it is necessary to increase the concentration ratio, disconnect the connector 3 from the concentration tank 4, rotate the concentration tank 4, empty the concentrate or dialysis fluid through the regulator 5, rotate it again, add new concentrate or dialysis fluid and screw on the connector 3 to continue the concentration or dialysis process.

[0022] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An exosome concentration and extraction apparatus, characterized in that, include: The container consists of a cap (1), an exosome tank (2), a connector (3), a concentration tank (4), and a regulator (5). The cap (1), exosome tank (2), connector (3), concentration tank (4), and regulator (5) are arranged sequentially from top to bottom. The exosome tank (2) and the concentration tank (4) are provided with a first threaded groove (6) and a second threaded groove (7) at their upper and lower ends, respectively. The connector (3) is provided with an annular groove (9) and a step (10) at its inner middle and lower inner sides, respectively. The step (10) is provided with a dialysis membrane (11) at its top. The dialysis membrane (11) is provided with a grid support (12) at its top. The grid support (12) is provided with an elastic card (13) on its outer side. The concentration tank (4) is connected to the regulator (5) through an adjustable component to adjust the amount of concentrate added.

2. The exosome concentration and extraction apparatus according to claim 1, characterized in that, The cover (1) is threadedly fixed to the exosome tank (2) via the first threaded groove (6), and the connector (3) is threadedly fixed to the concentration tank (4) via the second threaded groove (7). The exosome tank (2) is threadedly fixed to the connector (3) via the first threaded groove (6), and the concentration tank (4) is threadedly fixed to the connector (3) via the second threaded groove (7).

3. The exosome concentration and extraction apparatus according to claim 1, characterized in that, Sealing gaskets (8) are installed on both the upper and lower ends of the inner side of the connector (3). The upper sealing gasket (8) is located between the exosome tank (2) and the connector (3), and the lower sealing gasket (8) is located between the concentration tank (4) and the connector (3).

4. The exosome concentration and extraction apparatus according to claim 1, characterized in that, The elastic cards (13) are circular and there are several of them. The grid bracket (12) and the elastic cards (13) are engaged with the connector (3) through the annular slot (9).

5. The exosome concentration and extraction apparatus according to claim 1, characterized in that, The exosome container (2) is used to store the exosome solution to be concentrated or the sample to be dialyzed, and the concentration container (4) is filled with a concentrate.

6. The exosome concentration and extraction apparatus according to claim 1, characterized in that, The adjustable component includes a guide post (14) fixed to the top of the fixture and a knob (20) that rotates at the bottom of the fixture. The guide post (14) has a limiting groove (15) inside. A telescopic post (16) is movably arranged inside the guide post (14) through the limiting groove (15). A piston (18) is fixedly arranged on the top of the telescopic post (16). A screw (19) is fixedly arranged on the top of the knob (20). The screw (19) and the telescopic post (16) are connected by a threaded transmission.

7. The exosome concentration and extraction apparatus according to claim 6, characterized in that, The piston (18) is adapted to the inner diameter of the concentration tank (4), and the piston (18) is located inside the concentration tank (4) and is movable. A limit plate (17) is installed on the top of the guide column (14).

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