An integrated wall-adherent cell culture separation device

Abstract

The utility model relates to cell culture technical field, and disclose an integral type adherent cell culture separation device, including bearing frame, is equipped with the rotary plate in bearing frame top, the culture dish is placed in the both ends of rotary plate upper portion, the rotary motor for driving the rotation of rotary plate is fixedly installed in bearing frame, and the fixed frame is fixedly installed in one side of bearing frame, and the culture cover that is adapted with the culture dish of rotary plate end is fixedly installed in one side of fixed frame, and the culture cover is used for carrying out environment simulation to adherent cell, in the utility model, the rotary plate is driven to rotate through the rotary motor, and the culture cover is used for cultivating adherent cell in culture dish, and the culture dish for cultivating adherent cell is convenient to take and put, and through the effect of double -position alternation, improves the efficiency when cultivating adherent cell.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to an integrated adherent cell culture and separation device. Background Technology

[0002] Adherent cells (such as CHO cells, Vero cells, fibroblasts, etc.) need to attach to solid surfaces to grow, and their culture and isolation are the core links in biopharmaceutical production and cell experiments.

[0003] A search revealed a patent, CN216237095U, which discloses an integrated adherent cell culture separation device, comprising an incubator, a culture mechanism, and an adjustment mechanism. The incubator has a hinged lid at its upper opening, and two symmetrically distributed pads on its lower surface. The culture mechanism includes two mounting plates, each placed inside the incubator, with evenly distributed spring clips between them. Telescopic columns are located at the four corners of opposite inner sides of the mounting plates, and connecting blocks are provided between the telescopic ends of adjacent telescopic columns. The adjustment mechanism is located on the left and right sides between the two mounting plates and includes a PLC controller. The PLC controller is located at the lower front side of the incubator, and its input is electrically connected to an external power source. This integrated adherent cell culture separation device enables rapid separation of cells from the culture components.

[0004] The aforementioned patents have significant beneficial effects, but in practical application, they still have the following shortcomings: The aforementioned comparative documents describe adhering cells being cultured on the side of a flexible spring. When the cultured adhering cells need to be removed, the components used to culture the adhering cells must be completely removed from the incubator, and then the spring and other components must be reinserted into the incubator. This makes the culture of adhering cells quite cumbersome. Therefore, there is an urgent need in the art to improve the adhering cell culture and separation device to overcome the shortcomings of the prior art. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides an integrated adherent cell culture and separation device, which facilitates the handling of culture dishes used for adherent cell culture and improves the efficiency of adherent cell culture through the alternating effect of dual workstations.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an integrated adherent cell culture and separation device, comprising a support frame, a rotating plate above the support frame, culture dishes placed at both ends of the upper part of the rotating plate, a rotary motor for driving the rotating plate to rotate fixedly installed inside the support frame, a fixing frame fixedly installed on one side of the support frame, and a culture cover adapted to the culture dish at the end of the rotating plate fixedly installed on one side of the fixing frame, the culture cover being used to simulate the environment for adherent cells.

[0007] Preferably, the culture hood is equipped with a sensing device for detection and a heating device for heating. A water collection tank is provided at the lower part of the culture hood, and a water collection box is provided on one side of the fixing frame. A connecting pipe is provided between the water collection box and the water collection tank, and a pump body connected to the connecting pipe is provided in the water collection box.

[0008] Preferably, the upper part of the culture hood is equipped with a main controller, which is electrically connected to the sensing device, heating device, rotary motor and pump body in the water collection tank.

[0009] Preferably, the upper part of the support frame is provided with a support plate adapted to the rotating plate, and both ends of the upper part of the support plate are fixedly installed with telescopic devices that have a telescopic effect, and the rotating plate is fixedly installed between the telescopic ends of the two telescopic devices.

[0010] Preferably, the upper part of the rotating plate has two symmetrical positioning grooves, which are adapted to the size of the petri dish.

[0011] Preferably, a sealing ring is provided between the rotating plate and the culture hood, and the sealing ring is elastic.

[0012] Preferably, the rotating plate and the culture hood are provided with embedding grooves on their opposite sides, and the embedding grooves are adapted to the sealing rings.

[0013] To address the shortcomings of existing technologies, this invention provides an integrated adherent cell culture and separation device, overcoming the deficiencies of the prior art. The beneficial effects of this invention are as follows: In this invention, a rotating plate is driven to rotate by a rotary motor, and a culture hood is used to culture adherent cells in a culture dish. This facilitates the handling of culture dishes used for culturing adherent cells, and the alternating dual-station effect improves the efficiency of culturing adherent cells.

[0014] In this invention, by using a sensing device, a heating device, and a water collection tank that do not contact the culture dish, the cleanliness of the culture hood during the culture of adherent cells can be improved, the number of times the culture hood needs to be cleaned can be reduced, and the efficiency of repeated culture of adherent cells can be improved.

[0015] In this invention, the petri dish is secured in the positioning groove, which facilitates both the placement and removal of the petri dish and direct observation and use later.

[0016] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained by means of the structures pointed out in the description, claims, and drawings. Attached Figure Description

[0017] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic cross-sectional view of the present invention. Figure 3 for Figure 2 Enlarged structural diagram at point A in the middle; Figure 4 for Figure 3 Enlarged structural diagram at point B.

[0019] In the diagram: 1. Support frame; 2. Rotating plate; 3. Petri dish; 4. Rotary motor; 5. Fixing frame; 6. Culture cover; 7. Induction device; 8. Heating device; 9. Water collection tank; 10. Water collection container; 11. Connecting pipe; 12. Main controller; 13. Support plate; 14. Telescopic device; 15. Positioning groove; 16. Sealing ring; 17. Embedding groove. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] Please see Figures 1-4An integrated adherent cell culture and separation device includes a support frame 1, a rotating plate 2 above the support frame 1, and culture dishes 3 placed at both ends of the upper part of the rotating plate 2. A rotary motor 4 for driving the rotating plate 2 is fixedly installed inside the support frame 1. A fixing frame 5 is fixedly installed on one side of the support frame 1, and a culture cover 6 adapted to the culture dishes 3 at the end of the rotating plate 2 is fixedly installed on one side of the fixing frame 5. The culture cover 6 is used to simulate the environment for adherent cells. A sensing device 7 for detection and a heating device 8 for heating are installed inside the culture cover 6. A water collection tank 9 is located at the lower part of the culture cover 6, and a water collection box 10 is located on one side of the fixing frame 5. A connecting pipe 11 connects the water collection box 10 and the water collection tank 9. The water collection box 10 is equipped with a connection to the connecting pipe. The pump body is connected to the culture hood 6. The main controller 12 is located on the upper part of the culture hood 6. The main controller 12 is electrically connected to the induction device 7, the heating device 8, the rotary motor 4, and the pump body in the water collection tank 10. The support frame 1 is provided with a support plate 13 that is adapted to the rotating plate 2. Both ends of the upper part of the support plate 13 are fixedly installed with telescopic devices 14 that have a telescopic effect. The rotating plate 2 is fixedly installed between the telescopic ends of the two telescopic devices 14. A sealing ring 16 is provided between the rotating plate 2 and the culture hood 6. The sealing ring 16 is elastic. The rotating plate 2 and the culture hood 6 have embedded grooves 17 on their opposite sides. The embedded grooves 17 are adapted to the sealing rings 16. The upper part of the rotating plate 2 has two symmetrical positioning grooves 15 that are adapted to the size of the culture dish 3.

[0022] The specific implementation of this embodiment is as follows: When adhering cells need to be cultured, the adhering cell culture medium is placed in a culture dish 3, and the culture dish 3 is placed in the positioning groove 15 on the upper part of the rotating plate 2. The main controller 12 starts the rotating motor 4, which drives the support plate 13 and the rotating plate 2 to rotate. The culture dish 3 rotates to the appropriate position of the culture cover 6. The main controller 12 opens the telescopic device 14, and the telescopic end of the telescopic device 14 drives the rotating plate 2 to move upward. The sealing ring 16 is engaged in the two embedded grooves 17 to achieve a sealing effect inside the culture cover 6. The temperature and humidity inside the culture cover 6 are monitored in real time by the sensing device 7, and the temperature and humidity inside the culture cover 6 are displayed on the display screen on the main controller 12, so that the operator can easily observe the real-time situation inside the culture cover 6. The main controller 12 actively controls the pump in the water collection tank 10 to transport the water in the water collection tank 10 to the water collection trough 9 to ensure the humidity inside the culture cover 6, and also controls the heating device. 8. To maintain the temperature inside the culture hood 6, if external air is required, the rotating plate 2 can be moved downwards via the telescopic device 14, separating the culture hood 6 from the rotating plate 2, allowing external air to enter the culture hood 6 for adhering cell culture. When the adhering cells are almost fully cultured, they are placed in another culture dish 3, and the culture dish 3 is placed in another positioning slot 15 on the upper part of the rotating plate 2. At this time, the main controller 12 controls the telescopic device 14 to move the rotating plate 2 downwards, and the rotating motor 4 reverses to drive the rotating plate 2 to rotate, moving the culture dish 3 with the adhering cells fully cultured away from the culture hood 6. The culture dish 3 with the adhering cells to be cultured is then rotated to directly below the culture hood 6 for further culture. The culture dish 3 with the adhering cells cultured can then be removed for subsequent separation or direct observation. Through the above structural design, it is convenient to pick up and put down the culture dish 3 used for culturing adhering cells, and the efficiency of adhering cell culture is improved through the effect of alternating dual stations.

[0023] All of the electrical products mentioned above can be purchased from the market. They are mature technologies and have been fully disclosed, so they will not be repeated in the instruction manual. All of the electrical products mentioned above are equipped with power connection cords, and they are electrically connected to the main controller 12 and the 220V phase voltage (or 380V line voltage) through the power connection cords. The main controller 12 can be a conventional known device such as a computer that plays a control role.

[0024] Finally, it should be noted that in the description of this utility model, the terms "vertical," "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0025] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0026] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An integrated adherent cell culture and separation device, comprising a support frame (1), characterized in that, A rotating plate (2) is provided above the support frame (1). A culture dish (3) is placed at both ends of the upper part of the rotating plate (2). A rotating motor (4) for driving the rotating plate (2) to rotate is fixedly installed inside the support frame (1). A fixing frame (5) is fixedly installed on one side of the support frame (1). A culture cover (6) adapted to the culture dish (3) at the end of the rotating plate (2) is fixedly installed on one side of the fixing frame (5). The culture cover (6) is used to simulate the environment of adherent cells.

2. The integrated adherent cell culture and separation device according to claim 1, characterized in that, The culture hood (6) is equipped with a sensing device (7) for detection and a heating device (8) for heating. A water collection tank (9) is located at the lower part of the culture hood (6). A water collection box (10) is located on one side of the fixing frame (5). A connecting pipe (11) is provided between the water collection box (10) and the water collection tank (9). A pump body connected to the connecting pipe (11) is located inside the water collection box (10).

3. The integrated adherent cell culture and separation device according to claim 2, characterized in that, The upper part of the culture hood (6) is equipped with a main controller (12), which is electrically connected to the induction device (7), the heating device (8), the rotary motor (4) and the pump body in the water collection tank (10).

4. The integrated adherent cell culture and separation device according to claim 1, characterized in that, The upper part of the support frame (1) is provided with a support plate (13) that is compatible with the rotating plate (2). Both ends of the upper part of the support plate (13) are fixedly installed with telescopic devices (14) that have a telescopic effect. The rotating plate (2) is fixedly installed between the telescopic ends of the two telescopic devices (14).

5. The integrated adherent cell culture and separation device according to claim 1, characterized in that, Two symmetrical positioning grooves (15) are provided on the upper part of the rotating plate (2), and the positioning grooves (15) are adapted to the size of the petri dish (3).

6. The integrated adherent cell culture and separation device according to claim 1, characterized in that, A sealing ring (16) is provided between the rotating plate (2) and the culture hood (6), and the sealing ring (16) is elastic.

7. The integrated adherent cell culture and separation device according to claim 6, characterized in that, The rotating plate (2) and the culture cover (6) are both provided with embedding grooves (17) on their opposite sides, and the embedding grooves (17) are adapted to the sealing rings (16).

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