A human brain organoid culture plate

Abstract

The utility model discloses a kind of human brain organoid culture plates, including culture structure, the top of the culture structure is provided with cover plate structure, the inside of culture structure is provided with multiple culture dishes in front and back two sides horizontal array, by being provided with cover plate structure, additional protection and sealing property are provided for entire culture process, effectively prevent the invasion of external pollution source, to provide more safe and stable environment for the culture of human brain organoid, simultaneously, the connection tightness between cover plate and culture structure is strengthened, ensure that there is no loosening phenomenon in moving or operating process, in addition, quick installation and disassembly can also be realized, greatly improve the convenience of experimental operation, by being provided with culture structure, the culture demand of different types organoid can be satisfied, so that device can be freely combined and adjusted according to experimental requirement, improve the diversity and flexibility of experiment.

Description

Technical Field

[0001] This utility model relates to the field of biomedical device technology, specifically to a human brain organoid culture plate. Background Technology

[0002] Organoids, resembling tissues and organs, are models created using 3D in vitro cell culture systems to closely resemble their in vivo source tissues or organs. These 3D in vitro culture systems can replicate the complex spatial morphology of differentiated tissues and exhibit interactions and spatial positioning between cells and between cells and their surrounding matrix. Furthermore, they can demonstrate physiological responses similar to differentiated tissues and organs in vivo, exhibiting a very high degree of similarity to their source tissues. Compared to traditional 2D cell culture methods, 3D cultured organoids contain multiple cell types, transcending simple physical contact between cells to form closer, more efficient interactions between cells and between cells and the matrix, creating functional "micro-organoids" that can better simulate the development of organ tissues and their physiological and pathological states.

[0003] According to patent document CN216864195U, this utility model relates to a culture plate device for 3D brain organoid live cell observation, belonging to the technical problem of biological cell culture, and solving the technical problem of the limitations in the use of existing culture plate devices for 3D brain organoid live cell observation. The culture plate device includes a culture plate, which is composed of multiple culture units; a pipette, detachably mounted above any culture unit, with a piston inside, a first hole on the top wall, and several infusion ports on the bottom wall; a piston shaft, which passes through the first hole and is fixedly connected to the piston; and a handle, which is fixedly connected to the end of the piston shaft opposite to the piston. With this structure, the operator can assemble different numbers of culture units to form a culture plate as needed, thereby effectively controlling the number of culture chambers on the culture plate. Furthermore, through the pipette, piston, piston shaft, and handle, cell culture medium can be added more evenly to several culture chambers on the same culture unit.

[0004] In current technologies, the cover plate of a culture plate is usually simply placed on the culture plate. During use, the cover plate is prone to sliding or misalignment, which makes the culture medium susceptible to external contamination. This risk of contamination increases further, especially during long-term culture, affecting the stability of the culture environment and the accuracy of experimental results. In addition, the culture plates in current technologies are designed as a single piece with uniform apertures at the top, which cannot be freely combined and adjusted according to experimental needs. This cannot meet the culture requirements of different types of organoids and limits the diversity and flexibility of experiments. Summary of the Invention

[0005] The purpose of this invention is to provide a human brain organoid culture plate to solve the problems mentioned above. In the existing technology, the cover plate of the culture plate is usually simply placed on the culture plate. During use, the cover plate is prone to sliding or misalignment, which makes the culture medium susceptible to external contamination. Especially during long-term culture, the risk of contamination is further increased, affecting the stability of the culture environment and the accuracy of experimental results. In addition, the culture plates in the existing technology are of one piece, and the aperture of the top opening is uniform. They cannot be freely combined and adjusted according to experimental needs, which cannot meet the culture needs of different types of organoids and limits the diversity and flexibility of experiments.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a human brain organoid culture plate, comprising a culture structure, wherein a cover plate structure is provided on the top of the culture structure, and multiple culture dishes are arranged in a horizontal array on both the front and back sides inside the culture structure; The culture structure includes an installation component, and a fixing component is disposed inside the installation component; The cover plate structure includes a cover plate assembly, and the cover plate assembly has two symmetrical limiting components inside.

[0007] Preferably, the mounting assembly includes a culture plate, the top of which has a fixing groove, and multiple mounting grooves are horizontally arrayed on both the front and rear sides of the top of the fixing groove. Triangular blocks are fixedly connected to the front and rear sides of the left side of the inner wall of the mounting groove, and anti-slip pads are fixedly connected to the inclined surfaces of the triangular blocks. The outer walls of the two anti-slip pads are movably connected to the outer wall of the culture dish.

[0008] Preferably, a driving groove is provided at the middle position of the top of the fixing groove, and a limiting through hole is provided on the upper left and right sides of the inner wall of the driving groove, extending to the outside. A rectangular through hole is provided on the side of the front and rear mounting grooves that are close to each other, extending into the interior of the driving groove. A circular through hole is provided on the lower right side of the inner wall of the driving groove, extending to the outside.

[0009] Preferably, the fixing assembly includes an anti-slip knob, a connecting shaft fixedly connected to the left end of the anti-slip knob, the outer wall of the connecting shaft being movably connected to the inner wall of the circular through hole, a lead screw fixedly connected to the left end of the connecting shaft, the left end of the lead screw being rotatably connected to the inner wall of the drive groove, a plurality of sliders being threadedly connected to the outer wall of the lead screw, the outer wall of the sliders being movably connected to the inner wall of the drive groove, connecting blocks fixedly connected to both the front and rear sides of the sliders, the outer walls of the connecting blocks being movably connected to the inner wall of the rectangular through hole, fixing clamps fixedly connected to the sides of the two connecting blocks that are far apart from each other, the outer walls of the fixing clamps being movably connected to the inner wall of the mounting groove, and anti-slip pads II fixedly connected to the two inclined surfaces of the inner wall of the fixing clamps, the outer walls of the two anti-slip pads II being movably connected to the outer wall of the petri dish.

[0010] Preferably, the cover plate assembly includes a protective cover plate, the outer wall of which is movably connected to the inner wall of the fixing groove, and multiple sealing plates are horizontally arrayed and fixedly connected to the front and rear sides of the bottom of the protective cover plate, the outer wall of which is movably connected to the inner wall of the mounting groove.

[0011] Preferably, a fixing plate is fixedly connected to the middle position of the bottom of the protective cover, the outer wall of the fixing plate is movably connected to the inner wall of the drive groove, a connecting groove is opened on the top of the protective cover, and connecting through holes extending into the connecting groove are opened on both the left and right sides of the fixing plate.

[0012] Preferably, the limiting component includes a fixing sleeve, the outer wall of which is fixedly connected to the inner wall of the connecting groove, a sliding rod movably connected to the inner wall of the fixing sleeve, a spring sleeved on the front side of the outer wall of the sliding rod, a limiting plate fixedly connected to the front end of the sliding rod, a fixing insert plate fixedly connected to the front side of the limiting plate, the outer wall of the fixing insert plate movably connected to the inner wall of the connecting through hole and the limiting through hole, and a pull groove fixedly connected to the rear end of the sliding rod, the outer wall of the pull groove movably connected to the inner wall of the connecting groove.

[0013] Compared with the prior art, the beneficial effects of this utility model are: By incorporating a cover structure, the entire culture process is provided with additional protection and sealing, effectively preventing the intrusion of external contaminants. This creates a safer and more stable environment for the culture of human brain organoids. At the same time, the tightness of the connection between the cover and the culture structure is enhanced, ensuring that there will be no loosening during movement or operation. Furthermore, it allows for quick installation and disassembly, greatly improving the convenience of experimental operations.

[0014] By incorporating a culture structure, the device can meet the culture requirements of different types of organoids, allowing for free combination and adjustment according to experimental needs. This enhances the diversity and flexibility of experiments. Furthermore, it can stably fix the culture dish, preventing loosening or displacement during operation, thus effectively ensuring the stability of the culture environment. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the disassembled structure of this utility model; Figure 3 This is a schematic diagram of the culture structure of this utility model; Figure 4 This is a schematic diagram of the installation component of this utility model; Figure 5 This is a schematic diagram of the structure of the fixing component of this utility model; Figure 6This is a schematic diagram of the cover plate structure of this utility model; Figure 7 This is a schematic diagram of the cover plate assembly of this utility model; Figure 8 This is a cross-sectional view of the limiting component of this utility model.

[0016] In the diagram: 1. Culture structure; 2. Cover plate structure; 3. Petri dish; 11. Mounting assembly; 12. Fixing assembly; 111. Culture plate; 112. Fixing groove; 113. Mounting groove; 114. Triangular block; 115. Anti-slip pad one; 116. Drive groove; 117. Limiting through hole; 118. Rectangular through hole; 119. Circular through hole; 121. Anti-slip knob; 122. Connecting shaft; 123. Lead screw ; 124. Slider; 125. Connecting block; 126. Fixing clamp; 127. Anti-slip pad II; 21. Cover plate assembly; 22. Limiting assembly; 211. Protective cover plate; 212. Fixing plate; 213. Sealing plate; 214. Connecting groove; 215. Connecting through hole; 221. Fixing sleeve; 222. Sliding rod; 223. Spring; 224. Limiting plate; 225. Fixing insert plate; 226. Pull groove. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Please see Figure 1-2 A human brain organoid culture plate includes a culture structure 1, a cover structure 2 on the top of the culture structure 1, and multiple culture dishes 3 arranged in a horizontal array on both the front and back sides inside the culture structure 1.

[0019] Please see Figure 3-5The culture structure 1 includes an installation component 11, inside which a fixing component 12 is provided. The installation component 11 includes a culture plate 111. A fixing groove 112 is opened on the top of the culture plate 111. Multiple installation grooves 113 are horizontally arrayed on the front and rear sides of the top of the fixing groove 112. Triangular blocks 114 are fixedly connected to the front and rear sides of the left side of the inner wall of the installation groove 113. Anti-slip pads 115 are fixedly connected to the inclined surface of the triangular blocks 114. The outer walls of the two anti-slip pads 115 are movably connected to the outer wall of the culture dish 3. A driving groove 116 is opened at the middle position of the top of the fixing groove 112. Limiting grooves extending to the outside are opened on the upper left and right sides of the inner wall of the driving groove 116. A rectangular through hole 118 is provided on the side of the front and rear mounting grooves 113 that are close to each other, penetrating into the drive groove 116. A circular through hole 119 is provided on the lower right side of the inner wall of the drive groove 116, penetrating to the outside. The fixing assembly 12 includes an anti-slip knob 121. A connecting shaft 122 is fixedly connected to the left end of the anti-slip knob 121. The outer wall of the connecting shaft 122 is movably connected to the inner wall of the circular through hole 119. A lead screw 123 is fixedly connected to the left end of the connecting shaft 122. The left end of the lead screw 123 is rotatably connected to the inner wall of the drive groove 116. A plurality of sliders 124 are threadedly connected to the outer wall of the lead screw 123. The outer wall of the sliders 124 is movably connected to the inner wall of the drive groove 116. The slider 124 is connected to a connecting block 125 on both its front and rear sides. The outer wall of the connecting block 125 is movably connected to the inner wall of the rectangular through hole 118. A fixing clamp 126 is fixedly connected to the side of each connecting block 125 that is far apart from each other. The outer wall of the fixing clamp 126 is movably connected to the inner wall of the mounting groove 113. Anti-slip pads 127 are fixedly connected to the two inclined surfaces of the inner wall of the fixing clamp 126. The outer walls of the two anti-slip pads 127 are movably connected to the outer wall of the petri dish 3. By rotating the anti-slip knob 121, the connecting shaft 122 rotates within the circular through hole 119, thereby driving the lead screw 123 to rotate. The rotation of the lead screw 123 causes the slider 124 to rotate within the circular through hole 119. The drive groove 116 moves horizontally, and the movement of the slider 124 is transmitted to the fixing block 126 through the connecting block 125. This allows the fixing block 126 to clamp or release the petri dish 3 within the mounting groove 113. It is adaptable to various types of petri dishes 3, enabling the device to be freely combined and adjusted according to experimental needs, thus improving the diversity and flexibility of experiments. The anti-slip pad 127 enhances the friction between the fixing block 126 and the petri dish 3, ensuring that the petri dish 3 will not shift during use. The entire fixing assembly 12 can quickly fix and disassemble the petri dish 3 through a simple rotation operation, greatly improving the convenience and efficiency of experimental operations.

[0020] Please see Figure 6-8The cover structure 2 includes a cover assembly 21. The cover assembly 21 has two symmetrical limiting components 22 inside. The cover assembly 21 includes a protective cover 211. The outer wall of the protective cover 211 is movably connected to the inner wall of the fixing groove 112. Multiple sealing plates 213 are horizontally arrayed and fixedly connected to the front and rear sides of the bottom of the protective cover 211. The outer wall of the sealing plates 213 is movably connected to the inner wall of the mounting groove 113. A fixing plate 212 is fixedly connected to the middle position of the bottom of the protective cover 211. The outer wall of the fixing plate 212 is movably connected to the inner wall of the driving groove 116. A connecting groove 214 is opened at the top of the protective cover 211. Both sides of 212 are provided with connecting through holes 215 that penetrate into the connecting groove 214. The limiting component 22 includes a fixing sleeve 221. The outer wall of the fixing sleeve 221 is fixedly connected to the inner wall of the connecting groove 214. A slide rod 222 is movably connected to the inner wall of the fixing sleeve 221. A spring 223 is sleeved on the front side of the outer wall of the slide rod 222. A limiting plate 224 is fixedly connected to the front end of the slide rod 222. A fixing insert plate 225 is fixedly connected to the front side of the limiting plate 224. The outer wall of the fixing insert plate 225 is movably connected to the inner wall of the connecting through hole 215 and the limiting through hole 117. A pull groove 226 is fixedly connected to the rear end of the slide rod 222. The wall is movably connected to the inner wall of the connecting groove 214. By pulling the pull groove 226 in the two limiting components 22, the slide bar 222 slides on the inner wall of the connecting groove 214. This movement of the pull groove 226 causes the slide bar 222 to slide on the inner wall of the fixing sleeve 221. This causes the limiting plate 224 to drive the fixing insert plate 225 to slide on the inner wall of the connecting through hole 215 and exit from the limiting through hole 117. At the same time, the spring 223 is compressed, thereby releasing the fixed state between the protective cover plate 211 and the culture structure 1. At this time, the protective cover plate 211 can be easily removed, which is convenient for operating or replacing the culture dish 3. When it is necessary to reinstall the protective cover plate 211, the protective cover plate 211 can be easily removed. At step 1, align the fixing plate 212 with the drive groove 116 and insert it, while ensuring that the sealing plate 213 is aligned with and embedded in the mounting groove 113. Then, release the pull groove 226. Under the elastic force of the spring 223, the slide rod 222 moves in the opposite direction, pushing the limiting plate 224 to re-insert the fixing insert 225 into the limiting through hole 117, thus completing the fixing of the protective cover plate 211. This not only ensures a tight connection between the cover plate structure 2 and the culture structure 1, but also enhances the airtightness of the culture environment through the sealing plate 213, effectively preventing external contaminants from entering the culture area. It also facilitates quick disassembly and installation by experimental personnel, improving operational efficiency and experimental safety.

[0021] Working principle: By rotating the anti-slip knob 121, the connecting shaft 122 rotates on the inner wall of the circular through hole 119, which in turn drives the lead screw 123 to rotate. The rotation of the lead screw 123 causes the slider 124 to move horizontally within the drive groove 116. The movement of the slider 124 is transmitted to the fixing clamp 126 through the connecting block 125, so that the fixing clamp 126 can clamp or release the petri dish 3 within the mounting groove 113. It is adaptable to various types of petri dishes 3, allowing the device to be freely combined and adjusted according to experimental needs, thus improving the diversity and flexibility of experiments. The anti-slip pad 127 enhances the friction between the fixing clamp 126 and the petri dish 3, ensuring that the petri dish 3 will not shift during use. The entire fixing assembly 12 can quickly fix and disassemble the petri dish 3 through a simple rotation operation, greatly improving the convenience and efficiency of experimental operations. By pulling the grooves 226 in the two limiting components 22 to slide against the inner wall of the connecting groove 214, the sliding rod 222 moves against the inner wall of the fixing sleeve 221, causing the limiting plate 224 to slide against the inner wall of the connecting through hole 215 and exit from the limiting through hole 117. At the same time, the spring 223 is compressed, thereby releasing the fixed state between the protective cover 211 and the culture structure 1. At this time, the protective cover 211 can be easily removed, facilitating the operation or replacement of the culture dish 3. When it is necessary to reinstall the protective cover 211, the fixing plate 224 is removed. 12. Align the drive groove 116 and insert it, while ensuring that the sealing plate 213 is aligned with and embedded in the mounting groove 113. Then, release the pull groove 226. Under the elastic force of the spring 223, the slide rod 222 moves in the opposite direction, pushing the limiting plate 224 to re-insert the fixing insert 225 into the limiting through hole 117, thus completing the fixing of the protective cover 211. This not only ensures a tight connection between the cover structure 2 and the culture structure 1, but also enhances the airtightness of the culture environment through the sealing plate 213, effectively preventing external contaminants from entering the culture area. It also facilitates quick disassembly and installation by experimental personnel, improving operational efficiency and experimental safety.

[0022] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A human brain organoid culture plate, comprising a culture structure (1), characterized in that: The top of the culture structure (1) is provided with a cover plate structure (2), and multiple culture dishes (3) are arranged in a horizontal array on both the front and back sides inside the culture structure (1). The culture structure (1) includes an installation component (11), and a fixing component (12) is provided inside the installation component (11). The cover plate structure (2) includes a cover plate assembly (21), and the cover plate assembly (21) has two symmetrical limiting components (22) inside.

2. The human brain organoid culture plate according to claim 1, characterized in that: The installation assembly (11) includes a culture plate (111), the top of which is provided with a fixing groove (112), and multiple installation grooves (113) are horizontally arranged on the front and rear sides of the top of the fixing groove (112). Triangular blocks (114) are fixedly connected to the front and rear sides of the left side of the inner wall of the installation groove (113), and anti-slip pads (115) are fixedly connected to the inclined surface of the triangular blocks (114). The outer walls of the two anti-slip pads (115) are movably connected to the outer wall of the culture dish (3).

3. The human brain organoid culture plate according to claim 2, characterized in that: A driving groove (116) is provided at the middle position of the top of the fixed groove (112). A limiting through hole (117) extending to the outside is provided on the upper left and right sides of the inner wall of the driving groove (116). A rectangular through hole (118) extending to the inside of the driving groove (116) is provided on the side of the front and rear mounting grooves (113) that are close to each other. A circular through hole (119) extending to the outside is provided on the lower right side of the inner wall of the driving groove (116).

4. The human brain organoid culture plate according to claim 1, characterized in that: The fixing component (12) includes an anti-slip knob (121), the left end of which is fixedly connected to a connecting shaft (122). The outer wall of the connecting shaft (122) is movably connected to the inner wall of the circular through hole (119). The left end of the connecting shaft (122) is fixedly connected to a lead screw (123), the left end of which is rotatably connected to the inner wall of the drive groove (116). The outer wall of the lead screw (123) is threaded with a plurality of sliders (124), the outer wall of which is movably connected to the inner wall of the drive groove (116). The slider (124) is fixedly connected to the front and rear sides with connecting blocks (125). The outer wall of the connecting block (125) is movably connected to the inner wall of the rectangular through hole (118). The two connecting blocks (125) are fixedly connected to the side away from each other with a fixing clamp (126). The outer wall of the fixing clamp (126) is movably connected to the inner wall of the mounting groove (113). The two inclined surfaces of the inner wall of the fixing clamp (126) are fixedly connected to the anti-slip pads (127). The outer walls of the two anti-slip pads (127) are movably connected to the outer wall of the petri dish (3).

5. The human brain organoid culture plate according to claim 1, characterized in that: The cover plate assembly (21) includes a protective cover plate (211), the outer wall of the protective cover plate (211) is movably connected to the inner wall of the fixing groove (112), and multiple sealing plates (213) are horizontally arrayed and fixedly connected on both the front and rear sides of the bottom of the protective cover plate (211), and the outer wall of the sealing plate (213) is movably connected to the inner wall of the mounting groove (113).

6. The human brain organoid culture plate according to claim 5, characterized in that: A fixing plate (212) is fixedly connected to the middle position of the bottom of the protective cover (211). The outer wall of the fixing plate (212) is movably connected to the inner wall of the drive groove (116). A connecting groove (214) is opened on the top of the protective cover (211). A connecting through hole (215) is opened on both the left and right sides of the fixing plate (212) and extends into the connecting groove (214).

7. The human brain organoid culture plate according to claim 1, characterized in that: The limiting component (22) includes a fixed sleeve (221), the outer wall of the fixed sleeve (221) is fixedly connected to the inner wall of the connecting groove (214), a slide rod (222) is movably connected to the inner wall of the fixed sleeve (221), a spring (223) is sleeved on the front side of the outer wall of the slide rod (222), a limiting plate (224) is fixedly connected to the front end of the slide rod (222), a fixed insert plate (225) is fixedly connected to the front side of the limiting plate (224), the outer wall of the fixed insert plate (225) is movably connected to the inner wall of the connecting through hole (215) and the limiting through hole (117), and a pull groove (226) is fixedly connected to the rear end of the slide rod (222), the outer wall of the pull groove (226) is movably connected to the inner wall of the connecting groove (214).

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