Compositions and methods for improving payload regulation using activated T-cell nuclear factor (nfat) response elements

Abstract

Nucleic acid constructs comprising one or more activated T-cell nuclear factor (ATNF) response elements and an expressible nucleic acid sequence encoding a first manipulated polypeptide monomer are provided herein, wherein the first manipulated polypeptide monomer comprises a tunable polypeptide payload and at least one drug-sensitive domain (DSD). The abundance, availability, and / or biological activity of the payload are regulated by the interaction of an effective amount of immune cell-stimulating agent with the one or more ATNF response elements and by the interaction of an effective amount of ligand with the one or more DSDs.