QUICK CHARACTERIZATION OF PAM SEQUENCES
DE602016095549T2Active Publication Date: 2026-06-03PIONEER HI BREED INTERNATIONAL INC
Patent Information
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- PIONEER HI BREED INTERNATIONAL INC
- Filing Date
- 2016-05-12
- Publication Date
- 2026-06-03
AI Technical Summary
Technical Problem
Existing genome-editing technologies, such as designer zinc finger nucleases and TALENs, are costly and time-consuming due to low specificity and the need for redesigning nucleases for each target site, limiting their scalability and applicability to multiple genomic positions.
Method used
A method for rapid characterization of Protospacer-Adjacent-Motif (PAM) sequences using a library of circular double-stranded DNA plasmids, guide RNA, and Cas endonuclease, followed by amplification and sequencing to identify optimal PAM sequences, enabling precise genome editing.
Benefits of technology
Enables efficient, scalable, and cost-effective genome editing by accurately determining PAM sequences, facilitating targeted genome modifications and gene insertions.
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