CELL CULTURE PROCESS, PRODUCT PRODUCTION PROCESS AND CELL CULTURE DEVICE

DK3792344T3Active Publication Date: 2026-06-29FUJIFILM CORP
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Patent Information

Application Number
DK2019824606T
Authority / Receiving Office
DK · DK
Patent Type
Patents
Current Assignee / Owner
Priority Date
2019-05-24
Filing Date
2019-05-24
Publication Date
2026-06-29
Estimated Expiration
2039-05-24

AI Technical Summary

Technical Problem

Conventional cell culture methods struggle to maintain high cell concentrations in large-scale cell suspensions due to insufficient oxygen dissolution and increased cell damage from small bubble diameters and high gas flow rates, leading to a trade-off between oxygen transfer capacity and cell damage.

Method used

A cell culture method involving a sparger with an average hole diameter of 1 µm to 300 µm, a specific surface area to volume ratio, and a controlled gas flow rate to ensure efficient oxygen dissolution and minimize cell damage, using a culture vessel with a sintered metal sparger and a perfusion culture device for high cell concentration and product production.

Benefits of technology

Enables high cell concentration cultures with reduced cell damage and enhanced oxygen transfer capacity, improving productivity and product quality, particularly in large-scale cell suspension volumes.

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Abstract

Provide are a cell culture method which includes using a culture vessel which contains a cell suspension containing cells and discharging a gas including 30% by volume or more of oxygen into the cell suspension from a sparger disposed in the culture vessel to culture the cells, in which an average hole diameter in a gas discharge portion in the sparger is 1 µm or more and 300 µm or less, and a surface area A (m2) in the gas discharge portion in the sparger, a volume X (m3) of the cell suspension in the culture vessel, and a flow rate Q (m3 / min) of the gas that is discharged from the sparger satisfy a specific relationship, a product producing method including the cell culture method, and a cell culture device that enables the cell culture method.
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