Fungicidal products, methods, and applications thereof

Abstract

The present disclosure relates to fungicidal combinations and compositions comprising two or more fungicide compounds for controlling fungal disease in plants. Particularly, the present disclosure provides fungicidal products for controlling fungal disease in woody vines such grapes. The disclosure also relates to corresponding methods and uses thereof.

Description

“FUNGICIDAL PRODUCTS, METHODS, AND APPLICATIONS THEREOF” TECHNICAL FIELD

[0001] The present disclosure pertains to the field of agrochemicals, particularly towards agricultural pest (fungal pathogen) control and corresponding products. In particular, the present disclosure relates to fungicidal combinations and compositions comprising two or more fungicidal compounds for controlling fungal disease in plants. BACKGROUND OF THE DISCLOSURE

[0002] Background description includes information that may be useful in understanding the present disclosure. It is not an admission that any of the information provided herein is relevant to the presently claimed disclosure, or that any publication specifically or implicitly referenced is prior art to the presently claimed disclosure.

[0003] Plant diseases caused by infection of various phytopathogens such as fungi, bacteria, viruses, viroids, nematodes etc. cause deterioration of the quality of plants, considerable reduction of yield, and even death of plants, resulting in significant economic losses. Of these diseases, 70- 80% are caused by pathogenic fungi. In recent years, fungal disease of crops have become increasingly serious as they have severely affected crop yield and quality, and they have become an important bottleneck for the development of sustainable agriculture. Thus, there is a dire need to effectively control fungal disease in crops to achieve efficient and stable production of agricultural crops.

[0004] Fungicides are chemical compounds used to prevent, control, or eradicate the spread of fungi on crop or plants, and are important tools for managing plant diseases. Fungicides are grouped as contact, translaminar, or systemic in nature. Contact fungicides protect plant tissue topically, translaminar fungicides are redistributed from the upper sprayed leaf surface to the lower unsprayed surface, and systemic fungicides enter into plant tissue and are distributed by xylem vessels throughout the plant. Fungicides are also grouped together according to their mode of action.

[0005] Amongst various agricultural and horticultural crops / plants, grapes are one of the most important and widely cultivated fruit. The nutrients in grapes offer a number of possible health benefits and importantly, grapes are cultivated worldwide mostly to produce wine. However, grapes are susceptible to severe fungal disease. For instance, downy mildew, powdery mildew, grey mold, black rot and anthracnose are caused by fungi that result in reduced yield, quality and crop loss. In particular, downy mildew (Plasmopara viticola) is an extremely serious fungal disease of grapes that can result in severe crop loss. The pathogen attacks all green parts of the vine, especially the leaves. Severely infected leaves drop, and if enough defoliation occurs, the overwintering buds are more susceptible to winter injury. Thus, the fungus causes direct yield losses by rotting inflorescences, berries, clusters and shoots. Indirect losses result from premature defoliation of vines due to foliar infections.

[0006] While many fungicidal compounds belonging to various different chemical classes have been or are being developed for use in agricultural crops, however, many of these fungicides have one or more drawbacks such as low efficacy, high dosage requirements, crop tolerance or exhibiting activity only against particular phytopathogenic fungi or plant, and do not always meet the demands of the agricultural practice. Additionally, while use of fungicidal combinations or compositions comprising mixtures of different fungicidal compounds have also been tried, there nonetheless exists a need in the art for improved combinations and compositions of different fungicides having enhanced activity for controlling specific phytopathogenic fungi, and at the same time has low toxicity, high stability, and reduced cost. The present disclosure tries to address said need. OBJECTS OF THE DISCLOSURE

[0007] It is an object of the present disclosure to provide improved fungicidal product comprising two or more fungicides for controlling fungal disease in plants.

[0008] It is another object of the present disclosure to provide improved fungicidal product comprising two or more fungicides for controlling fungal disease in plants of Vitaceae family.

[0009] It is yet another object of the present disclosure to provide improved fungicidal product comprising two or more fungicides for controlling fungal disease in grapes.

[0010] Still another object of the present disclosure is to provide improved fungicidal product comprising two or more fungicides for controlling fungal disease in grapes such as downy mildew, powdery mildew, grey mold, black rot and anthracnose.

[0011] Still another object of the present disclosure is to provide improved fungicidal product comprising two or more fungicides for controlling fungal disease in grapes caused by Plasmopara spp.

[0012] Still another object of the present disclosure is to provide fungicidal product comprising two or more fungicides for controlling downy mildew in grapes caused by Plasmopara viticola.

[0013] It is also an object of the present disclosure to provide a method of preparation of fungicidal product comprising two or more fungicides.

[0014] It is another object of the present disclosure to provide a method of controlling fungal disease in crops, such as grapes.

[0015] It is still another object of the present disclosure to provide a method of controlling fungal disease in grapes such as downy mildew, powdery mildew, grey mold, black rot and anthracnose.

[0016] It is yet another object of the present disclosure to provide a method of controlling downy mildew disease in grapes caused by Plasmopara viticola.

[0017] It is an object of the present disclosure to provide improved combinations or compositions of fungicides that possess enhanced activity against phytopathogenic fungi as compared to individual fungicides.

[0018] It is another object of the present disclosure to provide combinations or compositions of fungicides that has lower application rates as compared to the individual fungicides.

[0019] It is yet another object of the present disclosure to provide an improved combinations or compositions of fungicides that has low phytotoxicity and high stability. SUMMARY

[0020] The present disclosure pertains to the technical field of agricultural combinations and compositions. In particular, the present disclosure pertains to fungicidal combinations and compositions comprising two or more fungicide compounds for controlling fungal disease in plants such as grapes.

[0021] An aspect of the present disclosure provides a fungicidal combination comprising at least two fungicides for controlling fungal disease in plants.

[0022] In another aspect, the present disclosure provides a fungicidal combination comprising at least two fungicides for preventing fungal disease in plants.

[0023] In some embodiments, the present disclosure provides a fungicidal combination for controlling phytopathogenic fungi in plants comprising: a) at least one carboxylic acid amide (CAA) compound; and b) at least one cyanoacetamide oxime compound.

[0024] In some embodiments, the present disclosure provides a fungicidal combination for controlling downy mildew in grapes caused by Plasmopara viticola, comprising: a) at least one carboxylic acid amide compound selected from Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) Cymoxanil.

[0025] An aspect of the present disclosure provides a fungicidal composition for controlling phytopathogenic fungi in plants, such as woody vines, comprising:a) at least one carboxylic acid amide compound; and b) at least one cyanoacetamide oxime compound.

[0026] In some embodiments, the present disclosure provides a fungicidal composition for controlling downy mildew in grapes caused by Plasmopara viticola, comprises: a) at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) at least one cyanoacetamide oxime compound.

[0027] An aspect of the present disclosure provides a method of controlling the growth of phytopathogenic fungi in grapes, a method of preventing a fungal disease in grapes , or a method of improving plant health in grapes comprising contacting or applying to foliage of the plants, a seed, or a locus thereof an effective amount of a fungicidal combination or a fungicidal composition of the present disclosure.

[0028] Further, aspect of the present disclosure relates to the use of the fungicidal combination or fungicidal composition of the present disclosure, for controlling fungal disease in plants, for preventing fungal disease in plants, and for improving plant health.

[0029] Various objects, features, aspects, and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments. DESCRIPTION OF THE DISCLOSURE

[0030] The following is a detailed description of embodiments of the present disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.

[0031] Groupings of alternative elements or embodiments of the disclosure disclosed herein are not to be construed as limitations. Each group member can be referred to and claimedindividually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and / or patentability.

[0032] Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises”, “comprising”, “includes” and “including” are to be construed in an open, inclusive sense that is as “including, but not limited to.”

[0033] Reference throughout this specification to “one embodiment” or “some embodiments” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in some embodiments” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0034] As used in the description herein and throughout the numbered embodiments or claims that follow, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise.

[0035] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, ratio and so forth, used to describe and claim certain embodiments of the disclosure are to be understood as being modified in some instances by the term “about”. Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the disclosure are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.

[0036] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.

[0037] The headings and abstract of the disclosure provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.

[0038] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided with respect to certain embodiments herein is intended merely to better illustrate the disclosure and does not pose a limitation on the scope of the disclosure otherwise claimed.

[0039] The following discussion provides many exemplary embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus, if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.

[0040] Various terms as used herein are described below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.

[0041] The term “about” as used herein encompasses variations of + / -10% and more preferably + / -5%, or any such variations that are appropriate for practicing the present disclosure to achieve desired effects.

[0042] The term “locus” as used herein means fields in or on which plants are growing, or where seeds of cultivated plants are sown, or where seed will be placed into the soil. The term also includes the vicinity of a desired crop in which fungal control is desired.

[0043] The term “crop” shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

[0044] The term “control” or “controlling” or “prevent” or “preventing” a fungal pathogen or a fungal disease refers to inhibiting or reducing the growth, reducing the ability of fungal pathogen to grow or reproduce or proliferate or spread, including killing (e.g., causing the morbidity or mortality, or reduced fecundity) of fungal pathogen. Controlling effects include all deviation from natural development, for example: killing, retardation, decrease or treatment of the disease.

[0045] The term “fungi”, “fungus”, “phytopathogenic fungi”, “phytopathogenic fungus” or the likes as used herein refers to fungi or fungus-like pathogens having the ability to cause infection or disease in plants.

[0046] The term “fungicide” as used herein means a compound that controls, modifies, inhibits or prevents the growth of fungi.

[0047] The term ‘fungicidally effective amount of the fungicide’ refers to an amount of the fungicide that kills or inhibits the phytopathogenic disease for which control or prevention is desired, in an amount not significantly toxic to the plant being treated.

[0048] The term “plant” refers to all physical parts of a plant including foliage / leaves, seeds, seedlings, saplings, roots, tubers, stems, stalks, and fruits. As used herein, the term “plant” and “crop” have been used interchangeably throughout the present disclosure.

[0049] Mandipropamid (MPD) is a carboxylic acid amide (CAA) compound in the mandelamide class and WDUJHWV^FHOOXORVH^V\QWKDVH^OLNH^3L&HV$^^WR inhibit cell wall biosynthesis inthe oomycete plant pathogen. Mandipropamid is chemically known as 2-(4-chlorophenyl)-N-[2- ^^-methoxy-4-prop-2-ynoxyphenyl)ethyl]-2-prop-2-ynoxyacetamide and represented as follows:

[0050] Alkyl urea-based fungicides such as Cymoxanil is a curative and preventive foliar fungicide. Cymoxanil is chemically known as (1E)-2-(ethylcarbamoylamino)-N-methoxy-2- oxoethanimidoyl cyanide and represented as follows:

[0051] Cymoxanil’s mode of action is presently unknown and works as local systemic. It penetrates rapidly and when inside the plant, it cannot be washed off by rain. It controls diseases during the incubation period and prevents the appearance of damage to the crop.

[0052] It was surprisingly found by the present inventors that the combination of carboxylic acid amide (CAA) based compound (cell wall biosynthesis inhibitor) and cyanoacetamide oxime compound as a fungicide for treating grape disease, results in an enhancement of fungicidalefficacy, and a surprising reduction in fungal disease incidence. For instance, the combination of carboxylic acid amide (CAA) based compound such as Mandipropamid and cyanoacetamide oxime compound such as Cymoxanil for treating grape disease results in an enhancement of fungicidal efficacy, and a surprising reduction in fungal disease incidence. As will be demonstrated in the examples below, the enhanced effect of the present fungicidal combination and composition thereof in treating grape diseases is far superior to similar combinations or compositions of the prior art.

[0053] The present disclosure provides a fungicidal combination comprising at least two fungicides for controlling growth of phytopathogenic fungi in plants.

[0054] The present disclosure also provides a fungicidal combination comprising at least two fungicides for treating fungal disease in plants.

[0055] In some embodiments, the fungicidal combinations of the present disclosure are effective in controlling fungal disease in wood vines, such as grapes, selected from downy mildew (caused by Plasmopara viticola), anthracnose (caused by Elsinoe ampelina), ripe rot (caused by Glomerella cingulata), powdery mildew (caused by Uncinula necator), rust (caused by Phakopsora ampelopsidis), and black rot (caused by Guignardia bidwellii), and botrytis (caused by Botrytis cinerea).

[0056] In some embodiments, the fungicidal combinations of the present disclosure are effective in controlling downy mildew in plants, such as, Grapes (caused by Plasmopara viticola), Cucurbit (caused by Pseudoperonospora cubensis), Brassica (caused by Hyaloperonospora parasitic), Basil (caused by Peronospora belbahrii), Hops (caused by Pseudoperonospora humuli), Roses (caused by Peronospora sparsa), and Spinach (caused by Peronospora effusa).

[0057] In some embodiments, the present disclosure provides a fungicidal combination for controlling fungal disease in plants caused by phytopathogenic fungi, preferably in order Peronosporales and more preferably of Plasmopara spp. Accordingly, the present fungicidal combination can be used to control fungal disease from one or more species of: Plasmopara

[0058] In some embodiments, the fungicidal combinations of the present disclosure are effective in controlling fungal disease in grapes. More particularly, the fungicidal combinations of the present disclosure are effective in controlling fungal disease in grape species selected from Vitis vinifera, Vitis amurensis, Vitis labrusca, Vitis riparia, Vitis rupestris, Vitis berlandeieri, Vitis aestivalis, Vitis cinerea, Vitis vulpine, Vitis rotundifolia, Vitis mustangensis, and combinations thereof.

[0059] In some embodiments, the fungicidal combinations of the present disclosure are effective in controlling fungal disease in grapes caused by Plasmopara spp.

[0060] In an exemplary embodiment, the fungicidal combinations of the present disclosure are effective in controlling downy mildew in grapes caused by Plasmopara viticola.

[0061] In some embodiments, the fungicidal combination for controlling fungal disease in plants / crops comprises two fungicides.

[0062] In some embodiments, at least one of the fungicides in the fungicidal combination is a carboxylic acid amide (CAA) compound.

[0063] In some embodiments, at least one of the fungicides in the fungicidal combination is a cyanoacetamide oxime compound.

[0064] In some embodiments, the present disclosure provides a fungicidal combination for controlling phytopathogenic fungi in plants comprising: a) at least one carboxylic acid amide (CAA) compound; and b) at least one cyanoacetamide oxime compound.

[0065] In some embodiments, the present disclosure provides a fungicidal combination for controlling phytopathogenic fungi in plants, comprising: a) at least one carboxylic acid amide compound, and b) at least one cyanoacetamide oxime compound

[0066] In an embodiment, the plant is grapes (Vitis spp.)

[0067] In some embodiments, the present disclosure provides a fungicidal combination for controlling phytopathogenic fungi in grapes comprising: a) at least one carboxylic acid amide (CAA) compound; and b) at least one cyanoacetamide oxime compound.

[0068] In some embodiments, the present disclosure provides fungicidal combination for controlling phytopathogenic fungi in plants caused by Plasmopara spp., comprising: a) at least one carboxylic acid amide compound, and b) at least one cyanoacetamide oxime compound.

[0069] In some embodiments, the present disclosure provides a fungicidal combination for controlling downy mildew in grapes (Vitis vinifera) caused by Plasmopara viticola, comprising: a) at least one carboxylic acid amide (CAA) compound; and b) at least one cyanoacetamide oxime compound.

[0070] In some embodiments, the carboxylic acid amide compound is selected from a class of compounds comprising cinnamic acid amides, valinamide carbamates, and mandelic acid amides.

[0071] In some embodiments, the carboxylic acid amide compound is mandelic acid amide.

[0072] In some embodiments, the carboxylic acid amide compound is selected from a group comprising Mandipropamid, Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, and Valifenalate.

[0073] In some embodiments, the carboxylic acid amide compound is Mandipropamid.

[0074] In some embodiments, the cyanoacetamide oxime compound is Cymoxanil.

[0075] In some embodiments, the present disclosure provides a fungicidal combination for controlling phytopathogenic fungi in plants comprising: a) at least one carboxylic acid amide compound selected from Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate and Mandipropamid; and b) at least one cyanoacetamide oxime compound.

[0076] In some embodiments, the present disclosure provides a fungicidal combination for controlling downy mildew in grapes caused by Plasmopara viticola, comprising: a) at least one carboxylic acid amide compound selected from Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) Cymoxanil.

[0077] In some embodiments, the fungicidal combination for controlling fungal disease in grapes comprises: a) Mandipropamid; and b) Cymoxanil.

[0078] In exemplary embodiments, the fungicidal combination for controlling downy mildew in grapes (Vitis vinifera) caused by Plasmopara viticola, comprises: a) Mandipropamid; and b) Cymoxanil.

[0079] In some embodiments, the fungicidal combination for controlling fungal disease in plants / crops of the present disclosure may further comprise additional fungicides selected from morpholine fungicides, triazole fungicides, acylamino acid fungicides, anilide fungicides, antibiotic fungicides, strobilurin fungicides, aromatic fungicides, arsenical fungicides, aryl phenyl ketone fungicides, benzimidazole fungicides, benzimidazole precursor fungicides, benzothiazole fungicides, bridged diphenyl fungicides, carbamate fungicides, conazole fungicides, copper fungicides, dicarboximide fungicides, dinitrophenol fungicides, dithiocarbamate fungicides, dithiolane fungicides, hydrazide fungicides, imidazole fungicides, inorganic fungicides, organophosphorus fungicides, organotin fungicides, oxathiin fungicides, oxazole fungicides, pyrimidine fungicides, pyrrole fungicides, quinoline fungicides, quinone fungicides, quinoxaline fungicides, thiadiazole fungicides, thiazole fungicides, thiazolidine fungicides, thiocarbamate fungicides, thiophene fungicides, triazine fungicides, triazolopyrimidine fungicides, urea fungicides, zinc fungicides, unclassified fungicides and mixtures thereof.

[0080] In another embodiment, the present disclosure provides a fungicidal combination for controlling fungal disease in plants comprising two or more fungicides each with different mode of actions.

[0081] In some embodiments, at least one of the fungicides is a cell wall biosynthesis inhibitor or an inhibitor of cellulose synthase.

[0082] In some embodiments, at least one of the fungicides has an unknown mode of action.

[0083] In some embodiments, at least one fungicide in the fungicidal combination for controlling fungal disease in plants has an unknown mode of action and is selected from cyanoacetamide oximes such as Cymoxanil.

[0084] In some embodiments, the fungicidal combination for controlling phytopathogenic fungi in grapes comprises: a) at least one cell wall biosynthesis inhibitor or an inhibitor of cellulose synthase; and b) at least one fungicide with unknown mode of action.

[0085] In some embodiments, the fungicidal combination for controlling phytopathogenic fungi in grapes comprises: a) at least one cell wall biosynthesis inhibitor or an inhibitor of cellulose synthase; and b) at least one fungicide with unknown mode of action.

[0086] In some embodiments, the fungicidal combination for controlling downy mildew in grapes caused by Plasmopara viticola comprises: a) at least one cell wall biosynthesis inhibitor or an inhibitor of cellulose synthase; and b) at least one fungicide with unknown mode of action.

[0087] The fungicidal combination for controlling or treating fungal disease in grapes of the present disclosure may be applied simultaneously as a tank mix or may be applied sequentially. The application may be made to plant or parts thereof, or the soil before emergence of the plants, either pre-planting or post-planting. The application may be made as a foliar spray at different timings during crop development, with either one or two applications early or late post-emergence. The fungicidal combinations as described above can be applied, for example, in a single “ready- mix” form, in a combined spray mixture composed of separate formulations of the single active ingredient components, such as a “tank-mix”, or in a combined use of the single active ingredients when applied in a sequential manner, i.e., one after the other within a reasonably short period, such as a few minutes, hours or days.

[0088] In some embodiments, the fungicidal combinations of the present disclosure comprising: a) at least one carboxylic acid amide compound; and b) at least one cyanoacetamide oxime compound, are employed in a ratio of 1:10 to 10:1.

[0089] In some embodiments, fungicidal combination of the present disclosure comprising: a) Mandipropamid and b) Cymoxanil are employed in ratio of 1:10 to 10:1.

[0090] In some embodiments, fungicidal combination of the present disclosure comprising: a) Mandipropamid and b) Cymoxanil are employed in ratios of 5:10 to 10:5, 2:5 to 5:2, 1.5:1 to 1:1.

[0091] In exemplary embodiments, fungicidal combination of the present disclosure comprising: a) Mandipropamid and b) Cymoxanil are employed in ratios of 1:1 or 1.04:1.

[0092] In exemplary embodiments, fungicidal combination of the present disclosure comprising: a) Mandipropamid and b) Cymoxanil are employed in a ratio of 1.04:1.

[0093] In exemplary embodiments, fungicidal combination of the present disclosure comprising: a) Mandipropamid and b) Cymoxanil are employed in a ratio of 1.^^:1

[0094] According to some embodiments, fungicides of the combinations as disclosed above, may be mixed at the time of application or on the point of application. The point of application refers to the locus of application of the said fungicides. The said locus could be an infected plant or a seed or any other plant propagation material, or an area adjacent to the said infected plant or a seed or any other plant propagation material and / or foliage.

[0095] While the preceding embodiments focus on fungicidal combinations for controlling fungal disease in plants, each of the said embodiments correspond to and completely fall within the purview of the fungicidal combinations for preventing fungal disease in plants.

[0096] The fungicidal combinations according to the present disclosure can be applied before or after infection of the useful plants or the propagation material thereof by the fungi.

[0097] The present disclosure further provides a fungicidal composition comprising at least two fungicides for controlling fungal disease in plants.

[0098] While the subsequent embodiments focus on fungicidal compositions, the features and characteristics of fungicide compounds, fungicides, fungal pathogen and disease, plant, mode of action, are as described by any of the embodiments above. For the sake of brevity, and avoiding repetition, each of those embodiments are not being reiterated here again. However, each of thesaid embodiments completely fall within the purview of the fungicidal composition, described herein.

[0099] In some embodiments, the fungicidal composition for controlling phytopathogenic fungi in grapes, comprises: a) at least one carboxylic acid amide compound; and b) at least one cyanoacetamide oxime compound.

[0100] In some embodiments, the fungicidal composition for controlling phytopathogenic fungi in grapes caused by Plasmopara spp., comprises: a) at least one carboxylic acid amide compound; b) at least one cyanoacetamide oxime compound; and

[0101] In some embodiments, the fungicidal composition for controlling downy mildew in grapes caused by Plasmopara viticola, comprises: a) at least one carboxylic acid amide compound; and b) at least one cyanoacetamide oxime compound.

[0102] In some embodiments, the fungicidal composition for controlling downy mildew in grapes caused by Plasmopara viticola, comprises: a) at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) at least one cyanoacetamide oxime compound.

[0103] In some embodiments, the fungicidal composition for controlling downy mildew in grapes caused by Plasmopara viticola, comprises: a) Mandipropamid; and b) Cymoxanil.

[0104] In an embodiment, Mandipropamid is a cell wall biosynthesis inhibitor.

[0105] In an embodiment, mode of action of Cymoxanil is unknown as per Fungicide Resistance Action Committee (FRAC).

[0106] In some embodiments, the fungicidal composition for controlling downy mildew in grapes (Vitis vinifera) caused by Plasmopara viticola, comprises: a) Mandipropamid; and b) Cymoxanil.

[0107] In some embodiments, the fungicidal composition for controlling fungal disease in grapes comprises agriculturally acceptable excipient.

[0108] In some embodiments, the fungicidal composition for controlling fungal disease in grapes comprises agriculturally acceptable excipient selected from a group comprising solvent, carrier, surfactant, dispersing agent, wetting agent, antifoam agent, stabilizing agent, pH modifier and combinations thereof.

[0109] In some embodiments, the agriculturally acceptable solvent is selected from aromatic compounds, such as xylene, toluene or alkylnaphthalenes, chlorinated aromatic compounds or chlorinated aliphatic hydrocarbons, such as chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons, such as cyclohexane or paraffins, for example mineral oil fractions, mineral and vegetable oils, alcohols, such as butanol or glycol, and also ethers and esters thereof, ketones, such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents, such as dimethylformamide and dimethylsulphoxide, and also water.

[0110] In some embodiments, the agriculturally acceptable carrier is selected from a solid carrier or a liquid carrier.

[0111] In some embodiments, the agriculturally acceptable liquid carrier is selected from water, toluene, xylene, petroleum ether, vegetable oils, acetone, methyl ethyl ketone, cyclohexanone, acid anhydrides, acetonitrile, acetophenone, amyl acetate, 2-butanone, butylene carbonate, chlorobenzene, cyclohexane, cyclohexanol, alkyl esters of acetic acid, diacetonealcohol, 1,2-dichloropropane, diethanolamine, p-diethylbenzene, diethylene glycol, diethylene glycol abietate, diethylene glycol butyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, N,N-dimethylformamide, dimethyl sulfoxide, 1,4-dioxane, dipropylene glycol, dipropylene glycol methyl ether, dipropylene glycol dibenzoate, diproxitol, alkylpyrrolidone, ethyl acetate, 2-ethylhexanol, ethylene carbonate, 1,1,1-trichloroethane, 2-heptanone, alpha-pinene, d- limonene, ethyl lactate, ethylene glycol, ethylene glycol butyl ether, ethylene glycol methyl ether, gamma-butyrolactone, glycerol, glycerol acetate, glycerol diacetate, glycerol triacetate, hexadecane, hexylene glycol, isoamyl acetate, isobornyl acetate, isooctane, isophorone, isopropylbenzene, isopropyl myristate, lactic acid, laurylamine, mesityl oxide, methoxypropanol, methyl isoamyl ketone, methyl isobutyl ketone, methyl laurate, methyl octanoate, methyl oleate, methylene chloride, m-xylene, n-hexane, n-octylamine, octadecanoic acid, octylamine acetate, oleic acid, oleylamine, o-xylene, phenol, polyethylene glycol, propionic acid, propyl lactate, propylene carbonate, propylene glycol, propylene glycol methyl ether, p-xylene, toluene, triethyl phosphate, triethylene glycol, xylenesulfonic acid, paraffin, mineral oil, trichloroethylene, perchloroethylene, ethyl acetate, amyl acetate, butyl acetate, propylene glycol methyl ether, diethylene glycol methyl ether, methanol, ethanol, isopropanol, and alcohols of higher molecular weight, such as amyl alcohol, tetrahydrofurfuryl alcohol, hexanol, octanol, ethylene glycol, propylene glycol, glycerol, N-methyl-2-pyrrolidone and combinations thereof.

[0112] In some embodiments, the agriculturally acceptable solid carrier is selected from talc, titanium dioxide, pyrophyllite clay, silica, attapulgite clay, kieselguhr, limestone, calcium carbonate, bentonite, calcium montmorillonite, cottonseed husks, wheat flour, soybean flour, pumice, wood flour, ground walnut shells, lignin and combinations thereof.

[0113] In some embodiments, the agriculturally acceptable surfactants may be selected from nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene alkyl (mono- or di-) phenyl ethers, polyoxyethylene (mono-, di- or tri-) styrylphenyl ethers, polyoxyethylene polyoxypropylene block copolymers, polyoxyethylene fatty acid (mono- or di-)esters, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, castor oil ethylene oxide adducts, acetylene glycol, acetylene alcohol, ethylene oxide adducts of acetylene glycol, ethylene oxide adducts of acetylene alcohol, alkyl glycosides, etc.; anionic surfactants, such as alkyl sulfates,alkylbenzene sulfonates, lignin sulfonates, alkyl sulfosuccinates, naphthalene sulfonates, alkylnaphthalene sulfonates, salts of formalin condensate of naphthalenesulfonic acid, salts of formalin condensate of alkylnaphthalene sulfonic acid, polyoxyethylene alkyl ether sulfuric acid or phosphoric acid ester salts, polyoxyethylene (mono- or di-) alkylphenyl ether sulfate or phosphate ester salts, polyoxyethylene (mono-, di- or tri)styrylphenyl ether sulfuric or phosphoric ester salts, polycarboxylates (for example, polyacrylic acid salts, polymaleic acid salts, a copolymer of maleic acid and olefin, etc.), polystyrene sulfonate, etc.; cationic surfactants, such as alkyl amine salts, alkyl quaternary ammonium salts, etc.; amphoteric surfactants of amino acid type, betaine type, etc.; silicone surfactants, fluorine surfactants, and combinations thereof.

[0114] In one embodiment, the fungicidal composition for controlling fungal disease in plants / crops may contain ionic and nonionic dispersing agents to enable disintegration of granules in water with ease, such as salts of polystyrenesulphonic acids, salts of polyvinylsulphonic acids, salts of naphthalenesulphonic acid / formaldehyde condensates, salts of condensates of naphthalenesulphonic acid, phenolsulphonic acid and formaldehyde, and salts of lignosulphonic acid, polyethylene oxide / polypropylene oxide block copolymers, polyethylene glycol ethers of linear alcohols, reaction products of fatty acids with ethylene oxide and / or propylene oxide, furthermore polyvinyl alcohol, polyvinylpyrrolidone, copolymers of polyvinyl alcohol and polyvinylpyrrolidone and copolymers of (meth)acrylic acid and (meth)acrylic esters, furthermore alkyl ethoxylates and alkylarylethoxylates. The preferred dispersing agents include sodium naphthalene sulfonate-formaldehyde condensate, polyethyleneglycol mono- [2,4,6-tris (1- phenylethyl] phenyl ether or a combination thereof.

[0115] In one embodiment, the fungicidal composition for controlling fungal disease in plants / crops may contain stabilizers. Such stabilizers may include carboxylic acids, such as citric acid and butenedioic acid or inorganic components such as sodium hydroxide, potassium hydroxide, and sodium dihydrogen phosphate dihydrate which may also act as a pH modifier.

[0116] In some embodiments, the fungicidal composition for controlling fungal disease in plants / crops of the present disclosure comprise at least one antifoaming agent which are usually employed for this purpose in agrochemical compositions. In some embodiments, the preferredantifoaming agents are selected from silicone oil and magnesium stearate or a suitable combination thereof.

[0117] In some embodiments, the fungicidal composition for controlling fungal disease in plants / crops of the present disclosure comprise at least one pH modifier selected from organic and inorganic components that are usually employed in agrochemical compositions to modify the pH. In a non-limiting embodiment, the pH modifier may be selected from potassium carbonate, potassium hydroxide, sodium hydroxide and sodium dihydrogen phosphate. However, the choice of a pH modifier is not particularly limiting.

[0118] In some embodiments, the fungicidal composition for controlling phytopathogenic fungi in grapes, comprises: a) at least one carboxylic acid amide compound; b) at least one cyanoacetamide oxime compound; and c) optionally at least one agriculturally acceptable excipient.

[0119] In some embodiments, the fungicidal composition for controlling downy mildew in grapes caused by Plasmopara viticola, comprises: a) Mandipropamid; b) Cymoxanil; and c) at least one agriculturally acceptable excipient.

[0120] In some embodiments, the fungicidal composition for controlling fungal disease in grapes , comprises: a) at least one carboxylic acid amide compound at an amount of about 1% to about 99%; b) at least one cyanoacetamide oxime compound at an amount of about 1% to about 99%; and c) an agriculturally acceptable excipient at an amount of about 1% to about 70%, by weight of the composition.

[0121] In some embodiments, the fungicidal composition for controlling fungal disease in plants such as grapes is in a solid dosage form or liquid dosage form.

[0122] In some embodiments, the fungicidal composition for controlling fungal disease in plants such as grapes is in a dosage form selected from a group comprising aerosols, capsule suspensions, cold-fogging concentrates, warm-fogging concentrates, encapsulated granules, fine granules, flowable concentrates for the treatment of seed, ready-to-use solutions, dustable powders, emulsifiable concentrates, oil-in-water emulsions, water-in oil emulsions, macrogranules, microgranules, oil-dispersible powders, oil-miscible flowable concentrates, oil- miscible liquids, foams, pastes, pesticide-coated seed, suspension concentrates, suspension emulsion concentrates, soluble concentrates, suspensions, wettable powders, soluble powders, dusts and granules, water-soluble granules or tablets, water-soluble powders for the treatment of seed, wettable powders, natural products and synthetic substances impregnated with active compound, and also microencapsulations in polymeric substances and in coating materials for seed, and also ULV cold fogging and warm-fogging formulations.

[0123] In another aspect, the present disclosure provides a process for preparation of the fungicidal composition, described herein.

[0124] In some embodiments, the process for preparation of fungicidal composition for controlling fungal disease in grapes comprises, mixing at least one carboxylic acid amide compound at an amount of about 1% to about 99%, at least one cyanoacetamide oxime compound at an amount of about 1% to about 99%, and an agriculturally acceptable excipient at an amount of about 1% to about 70%, by weight of the composition.

[0125] While the preceding embodiments focus on fungicidal compositions for controlling fungal disease in plants, each of the said embodiments correspond to and completely fall within the purview of the fungicidal compositions for preventing fungal disease in plants.

[0126] The present disclosure further relates to a method of controlling the growth of phytopathogenic fungi in plants, said method comprising contacting or applying to seeds, foliageor at the locus of plants an effective amount of a fungicidal combination or fungicidal composition, as described above.

[0127] The present disclosure also provides a method of preventing a fungal disease in plants, said method comprising contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination or fungicidal composition, as described above.

[0128] The present disclosure additionally provides a method of improving plant health in plants susceptible to phytopathogenic fungi, said method comprising contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination or fungicidal composition, as described above.

[0129] While the subsequent embodiments focus on method of controlling the growth of phytopathogenic fungi in plants, method of preventing a fungal disease in plants, and method of improving plant health, all the features and characteristics of fungicidal combination, fungicidal composition, fungicides, fungal disease, fungal pathogen, and plant are as described by any of the embodiments above. For the sake of brevity, and avoiding repetition, each of those embodiments are not being reiterated here again. However, each of the said embodiments completely fall within the purview of method of controlling the growth of phytopathogenic fungi in plants, method of treating a fungal disease in plants, and method of improving plant health.

[0130] In some embodiments, the method of controlling the growth of phytopathogenic fungi in grapes, method of preventing a fungal disease in plants, or the method of improving plant health in grapes comprises contacting or applying to foliage of the plants, a seed, or a locus thereof an effective amount of a fungicidal combination or a fungicidal composition, comprising: a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound.

[0131] In some embodiments, the method of controlling the growth of phytopathogenic fungi in grapes, method of preventing a fungal disease in plants, or the method of improving plant healthin grapes comprises contacting or applying to seeds, foliage or at the locus of grape plants an effective amount of a fungicidal combination or fungicidal composition, comprising: a) fungicidally effective amount of at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) fungicidally effective amount of Cymoxanil.

[0132] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination or a fungicidal composition comprising: a) fungicidally effective amount of at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) fungicidally effective amount of cyanoacetamide oxime compound which is Cymoxanil.

[0133] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil.

[0134] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising:a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid and the Cymoxanil are present in a ratio of 1:10 to 10:1.

[0135] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid and the Cymoxanil are present in a ratio of 1.04:1.

[0136] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid is applied at a rate in the range of 100 g a.i. / Ha to 150 g a.i. / Ha, and the Cymoxanil is applied at a rate in the range of 50 g a.i. / Ha to 140 g a.i. / Ha

[0137] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes, the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil,wherein the Mandipropamid is applied at a rate of 125 g a.i. / Ha, and the Cymoxanil is applied at a rate of 120 g a.i. / Ha.

[0138] In some embodiments of the method, the fungicidal combination is applied sequentially or simultaneously to the locus of application at different or same application rates or concentrations. Preferably, at least one fungicide is applied at the rate of about 100 to 150 g a.i. / Ha and at least one fungicide is applied at the rate of about 50 to 140 g a.i. / Ha. In some embodiments, the carboxylic acid amide based fungicide is applied at the rate of about 100 to 150 g a.i. / Ha and cyanoacetamide oxime based fungicide is applied at the rate of about 100 to 140 g a.i. / Ha.

[0139] In some embodiments of the method, the carboxylic acid amide based fungicide is Mandipropamid and is applied at the rate of about 100 g a.i. / Ha, about 105 g a.i. / Ha, about 110 g a.i. / Ha, about 115 g a.i. / Ha, about 120 g a.i. / Ha, about 125 g D^L^^+D^^DERXW^^^^^g a.i. / Ha, about ^^^^g a.i. / Ha, about 140 g a.i. / Ha, about 145 g a.i. / Ha, or about 150 g a.i. / Ha.

[0140] In an exemplary embodiment of the method, Mandipropamid is applied at the rate of about 125 g a.i. / Ha.

[0141] In some embodiments of the method, the cyanoacetamide oxime based fungicide is Cymoxanil and is applied at the rate of about 50 g a.i. / Ha, about 72 g a.i. / Ha, about 90 g a.i. / Ha, about 100 g a.i. / Ha, about 105 g a.i. / Ha, 108 g a.i. / Ha, about 110 g a.i. / Ha, about 115 g a.i. / Ha, about 120 g a.i. / Ha, about 125 g D^L^^+D^^DERXW^^^^^g D^L^^+D^^DERXW^^^^^g a.i. / Ha, or about 140 g a.i. / Ha.

[0142] In an exemplary embodiment of the method, Cymoxanil is applied at the rate of about 72 g a.i. / Ha.

[0143] In an exemplary embodiment of the method, Cymoxanil is applied at the rate of about 90 g a.i. / Ha.

[0144] In an exemplary embodiment of the method, Cymoxanil is applied at the rate of about 108 g a.i. / Ha.

[0145] In an exemplary embodiment of the method, Cymoxanil is applied at the rate of about 120 g a.i. / Ha.

[0146] In some embodiments of the method, the fungicidal combination for controlling fungal disease in grapes is applied as: a) Mandipropamid at the rate of about 125 g a.i. / Ha, and b) Cymoxanil at the rate of about 72 g a.i. / Ha.

[0147] In some embodiments of the method, the fungicidal combination for controlling fungal disease in grapes is applied as: a) Mandipropamid at the rate of about 125 g a.i. / Ha, and b) Cymoxanil at the rate of about 90 g a.i. / Ha.

[0148] In some embodiments of the method, the fungicidal combination for controlling fungal disease in grapes is applied as: a) Mandipropamid at the rate of about 125 g a.i. / Ha, and b) Cymoxanil at the rate of about 108 g a.i. / Ha.

[0149] In some embodiments of the method, the fungicidal combination for controlling fungal disease in grapes is applied as: a) Mandipropamid at the rate of about 125 g a.i. / Ha, and b) Cymoxanil at the rate of about 120 g a.i. / Ha.

[0150] In some embodiments of the method, the fungicidal combination for controlling downy mildew disease in grapes caused by Plasmopara viticola is applied as: a) Mandipropamid at the rate of about 125 g a.i. / Ha, and b) Cymoxanil at the rate of about 120 g a.i. / Ha.

[0151] In some embodiments of the method of controlling the growth of phytopathogenic fungi in grapes (Vitis spp.), the phytopathogenic fungi is Plasmopara viticola which causes downy mildew; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal composition comprising: a) fungicidally effective amount of Mandipropamid at an amount of about 1% to about 99%;b) fungicidally effective amount of Cymoxanil at an amount of about 1% to about 99%; and c) optionally, agriculturally acceptable excipient at an amount of about 1% to about 70%, by the weight of the composition.

[0152] In some embodiments of the method of preventing fungal disease in grapes, the fungal disease is downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination or a fungicidal composition comprising: a) fungicidally effective amount of at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) fungicidally effective amount of cyanoacetamide oxime compound which is Cymoxanil.

[0153] In some embodiments of the method of treating fungal disease in grapes, the fungal disease is downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil.

[0154] In some embodiments of the method of treating fungal disease in grapes (Vitis spp.), the fungal disease is downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination or a fungicidal composition comprising: a) fungicidally effective amount of at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) fungicidally effective amount of cyanoacetamide oxime compound which is Cymoxanil.

[0155] In some embodiments of the method of treating fungal disease in grapes (Vitis spp.), the fungal disease is downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil.

[0156] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination or a fungicidal composition comprising: a) fungicidally effective amount of at least one carboxylic acid amide compound selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid; and b) fungicidally effective amount of cyanoacetamide oxime compound which is Cymoxanil.

[0157] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil.

[0158] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil,wherein the Mandipropamid and the Cymoxanil are present in a ratio of 1:10 to 10:1.

[0159] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid and the Cymoxanil are present in a ratio of 1.04:1.

[0160] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid and the Cymoxanil are present in a ratio of 1.^^:1.

[0161] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the fungicidal combination is applied at a rate in the range from 50 g a.i. / Ha to 150 g a.i. / Ha.

[0162] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising:a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid is applied at a rate in the range from 100 g a.i. / Ha to 150 g a.i. / Ha, and the Cymoxanil is applied at a rate in the range from 50 g a.i. / Ha to 140 g a.i. / Ha.

[0163] In some embodiments of the method of improving plant health in grapes, where plant is susceptible to downy mildew caused by phytopathogenic fungi Plasmopara viticola; and said method comprises contacting or applying to seeds, foliage or at the locus of crop plants an effective amount of a fungicidal combination comprising: a) fungicidally effective amount of Mandipropamid; and b) fungicidally effective amount of Cymoxanil, wherein the Mandipropamid is applied at a rate of 150 g a.i. / Ha, and the Cymoxanil is applied at a rate of 108 g a.i. / Ha.

[0164] The methods of the present disclosure can be carried out in agricultural lands such as fields, lawns and orchards, or in non-agricultural lands. The present methods may be used to control diseases in agricultural lands for cultivating the plants without any phytotoxicity to the plant. The fungicidal combinations and compositions of the present disclosure do not show any sign or symptoms of any phytotoxicity and are safe at different growth stages of plants.

[0165] In some embodiments, the combinations or compositions of the present disclosure may be applied by various conventional treating techniques and machines, such as sprayers, fluidized bed techniques, the roller mill method, drones, rotostatic seed treaters, and drum coaters, spouted beds, etc. Pre- and post-coating procedures such as sizing etc., may also be carried out. Such procedures are known in the art. It is readily understood that plant propagation material will typically be treated only once it is removed from the plant and is ready to be re-sown.

[0166] In some embodiments, the treatment may occur before sowing of the plant propagation material so that the sown material has been pre-treated with the present fungicidal combination or composition. In particular, seed coating or seed pelleting are preferred in the treatment of the present fungicidal combination or composition according to the disclosure. As a result of thetreatment, the active ingredients in the combination and / or composition are adhered on to the seed and therefore available for disease control.

[0167] The present disclosure further relates to the use of the fungicidal combination or fungicidal composition described herein, for controlling fungal disease in plants.

[0168] The present disclosure also relates to the use of the fungicidal combination or fungicidal composition described herein, for preventing fungal disease in plants.

[0169] The present disclosure additionally relates to the use of the fungicidal combination or fungicidal composition described herein, for improving plant health.

[0170] While the subsequent embodiments focus on use of the fungicidal combination or fungicidal composition described herein for controlling fungal disease in plants, for treating a fungal disease in plants, and for improving plant health, all the features and characteristics of fungicidal combination, fungicidal composition, fungicides, fungal disease, fungal pathogen, and plant are as described by any of the embodiments above. For the sake of brevity, and avoiding repetition, each of those embodiments are not being reiterated here again. However, each of the said embodiments completely fall within the purview of use of the fungicidal combination or fungicidal composition described herein for controlling fungal disease in plants, for treating a fungal disease in plants, and for improving plant health.

[0171] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicidal composition for controlling growth of phytopathogenic fungi in plants comprising a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound for controlling downy mildew in grapes caused by Plasmopara viticola.

[0172] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicidal composition comprising Mandipropamid and Cymoxanil for controlling downy mildew in grapes caused by Plasmopara viticola.

[0173] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicide composition comprising a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound for preventing downy mildew in grapes caused by Plasmopara viticola.

[0174] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicide composition comprising Mandipropamid and Cymoxanil for preventing downy mildew in grapes caused by Plasmopara viticola.

[0175] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicidal composition comprising a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound for improving plant health where plant is grapes and is susceptible to downy mildew caused by Plasmopara viticola.

[0176] In some embodiments, the present disclosure relates to the use of the fungicidal combination or the fungicide composition comprising Mandipropamid and Cymoxanil for improving plant health where plant is grapes and is susceptible to downy mildew caused by Plasmopara viticola.

[0177] It is to be understood that the foregoing description is illustrative and not limiting. While considerable emphasis has been placed herein on particular features of this disclosure, it will be appreciated that various modifications can be made, and that many changes can be made in the preferred embodiments without departing from the principles of the disclosure. Those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein. Similarly, additional embodiments and features of the present disclosure will be apparent to one of ordinary skill in art based upon description provided herein.

[0178] Descriptions of well-known / conventional methods / steps and techniques are omitted so as to not unnecessarily obscure the embodiments herein. Further, the disclosure herein providesfor examples illustrating the above-described embodiments, and in order to illustrate the embodiments of the present disclosure, certain aspects have been employed. The examples used herein for such illustration are intended merely to facilitate an understanding of ways in which the embodiments may be practiced and to further enable those of skill in the art to practice the embodiments. Accordingly, following examples should not be construed as limiting the scope of the embodiments herein. EXAMPLES

[0179] While the foregoing description discloses various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope of the disclosure. The disclosure is not limited to the described embodiments, versions, or examples, which are included to enable a person having ordinary skill in the art to make and use the disclosure when combined with information and knowledge available to the person having ordinary skill in the art.data

[0180] Trials were performed to evaluate the fungicidal efficacy of the present combination (Mandipropamid + Cymoxanil) on Downy mildew in Vitis vinifera (Grape). Table 1: Results of treatment of grapes with different fungicides S. Fungicide Application % Disease on % Disease No. Treatment Rate (g Surface of Control A.i. / Ha) Leaf 7* 9* 7* 9* dAA dAA dAA dAA 1 Untreated -- 20.2 ^^^^ 0 0 2 Mandipropamid 125 1.7 2 91.58 94.20^ Mandipropamid 125 0 1.1 100 96.81 Cymoxanil 120 Note:- dAA stands for “days after application”.

[0181] The above results demonstrate that as compared to Mandipropamid alone, the present combination of Mandipropamid and Cymoxanil exhibits an overall significantly less spread (%) of the disease, thereby indicating improved control of downy mildew (Plasmopara viticola) in grapes. Particularly, the results show that the present fungicidal combination of Mandipropamid and Cymoxanil was able to eliminate the disease on leaf surface after 7 days of application. The combination of Mandipropamide and Cymoxanil does not exhibit any phytotoxicity.

[0182] Example 2 Trials were performed to evaluate the fungicidal efficacy of the present combination (Mandipropamid + Cymoxanil) on Downy mildew in Vitis vinifera (Grape) Trt Treatment Rate (g % Control No. Name AI / ha) 9 dAA 10 dAA 11 dAA 1 Untreated Check 0 0 0 2 Mandipropamid 100 ^^^^^ 48.51 ^^^^^ ^ Mandipropamid 125 65.97 50.45 42.71 4 Mandipropamid 150 69.22 ^^^^^ ^^^^^ 5 Cymoxanil 72 ^^^^^ 52.51 42.91 6 Cymoxanil 90 ^^^^ ^^^^^ 46.64 7 Cymoxanil 108 74.25 ^^^^ 51.14 8 Mandipropamid 100 76.48 65.4 52.44 Cymoxanil 72 9 Mandipropamid 125 79.78 67.95 56.88 Cymoxanil 90 10 Mandipropamid 150 ^^^^^ ^^^^^ 62.19 Cymoxanil 108 The above results demonstrate that as compared to Mandipropamid alone, the present combination of Mandipropamid and Cymoxanil exhibits an overall significantly less spread (%) of the disease, thereby indicating improved control of downy mildew (Plasmopara viticola) in grapes.ADVANTAGES OF THE PRESENT DISCLOSURE

[0183] The present fungicidal combination and fungicidal composition for controlling fungal disease in plants, preferably grapes, that demonstrates enhanced antifungal efficacy, reduced toxicity, reduced use rates, lesser damage to the environment.

[0184] The methods employed by the present disclosure indicate sustained and effective control and treatment of fungal disease in plants, preferably downy mildew in grapes.

[0185] The methods employed by the present disclosure further result in enhancing plant yield and improving plant health, preferably in grapes susceptible to downy mildew caused by Plasmopara spp.

[0186] The present fungicidal combination and fungicidal composition for controlling fungal disease in crop plants, preferably downy mildew in grapes, demonstrates that combining a cell wall biosynthesis inhibitor (for e.g. Mandipropamid) with a fungicide with unknown mode of action (for e.g. Cymoxanil) leads to an enhanced fungicidal activity. The foregoing description of the specific embodiments reveal the general nature of the embodiments herein that others can, by applying current knowledge, readily modify and / or adapt for various applications such specific embodiments without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments. It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments in this disclosure have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the embodiments as described herein. Throughout this specification, the term ‘combinations thereof’ or ‘any combination thereof’ or ‘any combinations thereof’ are used interchangeably and are intended to have the same meaning, as regularly known in the field of patents disclosures.As regards the embodiments characterized in this specification, it is intended that each embodiment be read independently as well as in combination with another embodiment. For example, in case of some embodiments ^^UHFLWLQJ^^^DOWHUQDWLYHV^$^^%^DQG^&^^some embodiments 2 UHFLWLQJ^^^DOWHUQDWLYHV^'^^(^DQG^)^DQG^some embodiments ^^UHFLWLQJ^^^DOWHUQDWLYHV^*^^+^DQG^,^^LW^LV^ to be understood that the specification unambiguously discloses embodiments corresponding to combinations A, D, G; A, D, H; A, D, I; A, E, G; A, E, H; A, E, I; A, F, G; A, F, H; A, F, I; B, D, G; B, D, H; B, D, I; B, E, G; B, E, H; B, E, I; B, F, G; B, F, H; B, F, I; C, D, G; C, D, H; C, D, I; C, E, G; C, E, H; C, E, I; C, F, G; C, F, H; C, F, I, unless specifically mentioned otherwise. Any discussion of documents, acts, materials, devices, articles and the like that has been included in this specification is solely for the purpose of providing a context for the disclosure. It is not to be taken as an admission that any or all of these matters form a part of the prior art base or were common general knowledge in the field relevant to the disclosure as it existed anywhere before the priority date of this application.

Claims

WE CLAIM:

1. A fungicidal combination for controlling phytopathogenic fungi in plants caused by Plasmopara spp., comprising: - at least one carboxylic acid amide compound; and - at least one cyanoacetamide oxime compound.

2. The fungicidal combination as claimed in claim 1, wherein carboxylic acid amide compound is selected from a group comprising Dimethomorph, Flumorph, Benthiavalicarb, Iprovalicarb, Valifenalate, and Mandipropamid. ^^ The fungicidal combination as claimed in claim 1, wherein cyanoacetamide oxime compound is Cymoxanil.

4. The fungicidal combination as claimed in claim 1, wherein the plant is grapes (Vitis spp.).

5. The fungicidal combination as claimed in claim 1, wherein the fungal disease is downy mildew.

6. The fungicidal combination as claimed in claim 5, wherein downy mildew is caused by the phytopathogenic fungus of Plasmopara spp.

7. The fungicidal combination as claimed in claim 1, wherein the fungicidal combination is employed in a ratio of 1:10 to 10:

1.

8. The fungicidal combination as claimed in claim 1, wherein the carboxylic acid amide compound is applied at the rate of about 100 to 150 g a.i. / Ha.

9. The fungicidal combination as claimed in claim 1, wherein the cyanoacetamide oxime compound is applied at the rate of about 50 to 140 g a.i. / Ha.

10. A method for controlling growth of phytopathogenic fungi in plants, said method comprising contacting or applying to foliage of the plants, a seed, or a locus thereof an effective amount of a fungicidal combination or a fungicidal composition comprising: a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound.

11. A fungicidal combination, comprising: - at least one carboxylic acid amide compound; and - at least one cyanoacetamide oxime compound; for controlling growth of phytopathogenic fungi in plants or improving plant health.

12. The fungicidal combination as claimed in claim 11, comprising: - Mandipropamid; and - Cymoxanil for controlling growth of phytopathogenic fungi in plants or improving plant health. ^^^ Use of a fungicidal combination or a fungicidal composition for controlling growth of phytopathogenic fungi in plants, wherein said fungicide combination or fungicide composition comprises: - at least one carboxylic acid amide compound, and - at least one cyanoacetamide oxime compound.

14. A method of improving plant health in plants susceptible to phytopathogenic fungi, said method comprising contacting or applying to foliage of the plants, a seed, or a locus thereof an effective amount of a fungicidal combination or a fungicidal composition comprising: a) at least one carboxylic acid amide (CAA) compound, and b) at least one cyanoacetamide oxime compound.

15. A fungicidal composition for controlling phytopathogenic fungi in plants caused by Plasmopara spp., comprising: - at least one carboxylic acid amide compound; - at least one cyanoacetamide oxime compound; and - at least an agrochemically acceptable excipient.

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