Composition combining a thermal water and a lactobacillus acidophilus extract and activity thereof for strengthening the skin barrier and combating skin aging

Abstract

The invention relates to a cosmetic and / or dermatological composition. It is characterized in that it comprises, in a physiologically acceptable medium, a thermal water with a mineral content of greater than 20 mg / l, and a concentration of between 1% and 10% of a Lactobacillus acidophilus extract by weight relative to the total weight of the composition. The invention also relates to the uses of such a composition for protecting, strengthening and / or repairing the barrier function of the skin and reducing the feelings of discomfort on sensitive to reactive skin or even atopic skin, for combating skin aging and for improving the skin microbiota.

Description

[0001]COMPOSITION COMBINING A THERMAL SPRING WATER AND AN EXTRACT OF LACTOBACILLUS ACIDOPHILUS AND ITS ACTIVITY OF STRENGTHENING THE SKIN BARRIER AND AGAINST SKIN AGING TECHNICAL FIELD The invention relates to a cosmetic and / or dermatological composition comprising a thermal spring water combined with an extract of Lactobacillus acidophilus, and its use for skin care. PRIOR ART The skin is a constantly renewing organ that covers the surface of the body, which constitutes the interface between the body and the external environment. Its purpose is to protect the body, in particular from external aggressions, but also to combat dehydration by limiting the diffusion of water. The skin is thus a vital organ composed of several layers (dermis, proliferative layers and stratum corneum), which provide protective, sensitive, immune, metabolic or thermoregulatory functions. The skin, like other organs,is subject to aging. The appearance of the skin can be modified by internal alterations (intrinsic aging, diseases and hormonal changes such as pregnancy) or external (environmental factors, such as pollution, sunlight, pathogens, temperature variations, etc.). As a result of these alterations, wrinkles and fine lines, pigmentation defects, dryness or even dehydration of the skin, thinning of the epidermis, elastosis, imperfections, age spots, etc. may appear. All these changes affect not only the skin, but also the keratinous appendages such as nails and hair. Although the entire structure of the skin actively participates in the body's defense, this skin barrier is largely ensured by the epidermis. The epidermis is an epithelium subdivided into several layers or strata, from the basal layer just above the dermis,crossing the granular and spinal layers, up to the upper layer, the horny layer or stratum corneum. The role of the epidermis is essential in preventing the loss of water and other components of the body to its external environment and in protecting the body against a variety of environmental aggressions. Its main function is therefore to protect the body from external threats by establishing physical, chemical, biochemical and immunological barriers to them, while maintaining a certain capacity for exchange between the external and internal environments. Also, the very compact structure of the horny layer forms a barrier to external aggressions and gives the skin its impermeability. Indeed, the lipids of the intercellular cement (polyunsaturated fatty acids, cholesterol, ceramides) and keratin ensure its waterproofing and slow the evaporation of water from the deeper layers. For this effect to be optimal,The stratum corneum must be perfectly intact: if it is hyperhydrated (under an occlusive patch for example), the skin becomes permeable in both directions; if it is dried out, the barrier cracks and becomes vulnerable. The skin's hydration status results from a balance between diffusion and evaporation of water. The margin is very narrow to guarantee optimal hydration: plus or minus 2% around the average value of 13%. The skin is considered dry as soon as hydration is less than 10%. If water evaporation is permanent, it is constantly renewed by diffusion mechanisms. It is the nutritional water intake as well as the intake via the skin that compensates for these losses: the water from the bloodstream supplements the water reserve that constitutes the dermis,water that will continue to diffuse into the upper layers of the skin. Hydration of the skin is therefore essential for it to ensure its barrier function. The water in the skin is found in different states: non-mobilizable water, bound to biological molecules, and mobilizable water that can transit through the different layers of the epidermis. The dermis constitutes the skin's water reservoir: it contains approximately 80%. It can store water because it is largely made up of a gel of proteoglycans (Extra Cellular Matrix) which encases its constituent proteins (collagen, elastin, etc.). This gel is notably composed of hyaluronic acid which has the capacity to fix water (up to 1000 times its weight in water),which retains it mainly at the level of the dermis. This large water reserve ensures the "tension" of the skin. A small fraction of this water is called "mobilizable water". It is able to cross the upper layers of the epidermis by passive diffusion. It also helps hydrate the corneocytes, which in addition to their richness in keratin contain their own natural hydration factor or NMF (Natural Moisturizing Factor) made up of several molecules, such as urea, amino acids from the degradation of filaggrin, lactic acids, sugars, mineral salts, with water retention properties. NMF represents up to 20-30% of the dry matter of the stratum corneum and helps it to remain hydrated and the skin to not have a dry feel and to maintain its suppleness. With age, skin dryness increases due to a decrease in NMF. Similarly,Any mechanical or chemical alteration of corneocytes causes NMF to leak out of the cell and reduces their ability to retain water. The level of NMF components is reduced after washing the skin with soap. A reduction in NMF leads to dry skin and a disruption of its barrier function. The skin, less protected, then becomes much more susceptible to damage caused by irritants. NMF is therefore considered the essential component in the regulation of epidermal homeostasis and there is a need to strengthen it. NMF helps maintain the hydration of the stratum corneum and the suppleness of the skin. When the barrier function is disrupted, the epidermis triggers different signaling pathways to restore the normal state of the skin. Lipid secretion is stimulated and filaggrin is degraded to NMF. These two actions take place simultaneously to restore the barrier function. Therefore,A decrease in filaggrin reveals an alteration of the skin barrier. The main function of this protein lies in the formation of the skin barrier. It is responsible for the aggregation and compaction of intermediate keratin filaments, playing a role in the flattening of cells (corneocytes). The bundles of intermediate keratin filaments aggregated by filaggrin bind to structural proteins through the action of transglutaminases. Filaggrin is said to be one of the "youth proteins", like collagen and elastin. In the stratum corneum, its role is twofold: on the one hand, it helps to conserve water in the skin, and on the other hand, it participates in the protection of the epidermis from solar radiation. It is a marker of improvement in epidermal or keratinocyte differentiation. In addition, since lipids are synthesized in keratinocytes during this epidermal differentiation,Filaggrin is also involved in the metabolism and organization of skin lipids (lipid bilayer). According to the literature, 27.5% of Caucasian Americans, 48% of Europeans, 31.4% of Chinese and 20% of Japanese people with atopic dermatitis have mutations in the filaggrin gene. Around fifty mutations causing a loss of function have been identified. Nearly half of people with the most severe forms of eczema have a filaggrin deficiency and patients with genetic mutations in the gene are three times more likely to have atopic dermatitis. Indeed, a mutation in the filaggrin gene can interfere with the skin's ability to act as a barrier, making it more prone to allergies and infections. Furthermore,Filaggrin deficiency in atopic dermatitis is thought to be linked to an increase in colonization by Staphylococcus aureus. Filaggrin is therefore essential in the formation and function of the skin barrier. Mutations in the filaggrin gene are responsible for skin disorders and increase the risk of developing pathologies such as atopic dermatitis, which is characterized by chronic eczema, with significant pruritus and dry skin (xerosis). Also, the capacity to produce collagen decreases with age. Our skin loses an average of 1% each year, starting from the age of 30. Because of this, the skin becomes fragile, withers and loses its firmness. However, it is known that repeated use of certain topical medications (dermocorticosteroids, retinoids, etc.) can dry out the skin, which, weakened,cannot retain water properly. There are also pathological factors such as alterations in the skin barrier (dermatoses, burns, etc.) which accelerate water loss. Finally, when water intake is insufficient, dehydration of the skin is observed. Water is particularly important in the skin. It is therefore essential in cosmetics, both as a solvent and for its moisturizing role. There are two main possibilities for increasing skin hydration: - Reduce water evaporation, and - Increase water fixation. When a cosmetic product is formulated, it must maintain the integrity of the skin while being pleasant to use for the consumer (texture, smell,color...). It must also respect the pH of the epidermis. Hydration can be easily restored by temporarily using a richer dermocosmetic treatment that will meet the skin's needs. Several types of substances are thus used in the formulations: - hydrophobic film-forming agents, which remain on the surface of the skin after evaporation of the aqueous phase of the emulsion. They form a more or less occlusive impermeable barrier that reduces the evaporation of intrinsic water. Examples include petroleum jelly, paraffin, beeswax, lanolin, cetyl and stearyl alcohol, fatty alcohols (caprylyl glycol, behenyl alcohol, cetearyl alcohol, octyldodecanol), silicones (cyclohexasiloxane, dimethicone), squalane, synthetic fatty esters (cetearyl isononanoate) and fatty acids (stearic and palmitic acid, lauroyl lysine), triglycerides (apricot oil, sweet almond oil, soybean oil),and shea butter; - intercellular cement correctors, which are incorporated into the intercorneocyte spaces to reunite the cells and limit the diffusion of water. Illustrative examples include ceramides, phospholipids, polyunsaturated fatty acids: γ linolenic acid (borage oil, camelina oil), linoleic acid (safflower oil, evening primrose oil); - hydrophilic film-forming agents, which have a high capacity to fix water within the horny layer. These are like "gels" that retain water on the surface of the skin, and which increase the moisturizing capacities of the other associated active ingredients. Illustrative examples include glycosaminoglycans (hyaluronic acid), collagen, chitosan, galactomannans,polyvinyl alcohol...; - hygroscopic "hydrating" substances that capture water in the environment to bring it to the skin. They are widely used and require their association with lipophilic agents. Examples include glycerin, glycerol (stearate), sorbitol, propylene glycol. These substances are used to create emulsions. Their aqueous phase is essentially composed of water, the first element that appears in the composition of dermocosmetics. In general, it is demineralized and decontaminated water that serves as a solvent. Substituting thermal water allows you to benefit from its unique physicochemical composition, which makes it an active ingredient in its own right and not just a solvent. However, its use requires complex formulations with a lipophilic phase to be effective, as the minerals must be in contact with the skin,which is a hydrophobic surface. The presence of surfactants and emulsifiers (such as polyethylene glycol, polysorbate, potassium alkyl phosphate, etc.) is however essential to allow the miscibility of the phases and ensure the stability of the emulsion. Various texturizing agents are also incorporated. Furthermore, to provide a biological solution for the protection of the skin's barrier function and its repair following an external aggression, patent document EP2986347 discloses a dermatological composition for topical use, comprising the combination of a culture supernatant and a cell lysate, said supernatant and lysate being derived from a culture of Lactobacillus pentosus (and more particularly the strain Lactobacillus pentosus CNCM I-4730 as filed on April 4, 2013 in accordance with the Budapest Treaty with the National Collection of Cultures of Microorganisms (CNCM)),for its use in the treatment of irritated or inflammatory skin conditions, said supernatant and said cell lysate originating from a culture of Lactobacillus pentosus in stationary phase, after centrifugation of the culture medium to obtain a supernatant and a biomass, separation of the supernatant and the biomass, complete cell lysis of the biomass to obtain the lysate, and mixing said supernatant and said lysate in a weight ratio of supernatant to lysate of 1 to 50, said combination being present in a concentration of 0.1 to 10% by weight relative to the total weight of the composition. The authors have demonstrated that to possess such properties, said culture extract must combine both a supernatant and a cell lysate of the culture, this combination making it possible to bring together proteins, peptides,polysaccharides and short-chain amino and organic acids and more generally all the compounds forming the bacterial cell and the metabolites produced by L. pentosus, which, in combination, improve the barrier function of the stratum corneum or accelerate its recovery when it is impaired, by nourishing the NMF. Also known is document WO2022 / 013897 which discloses a dermatological composition comprising 0.1-1% (w / w) of a mixture of lysates from the fermentation of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum and a lysate of Streptococcus thermophilus. This is a dermatological composition between skin care and colored makeup base for the needs of those who want a "hybrid" solution halfway between skin care and makeup to rebalance the skin, increase the degree of hydration of the skin,the degree of elasticity and at the same time reduce skin discoloration. It has also been shown that a Bifidobacterium longum lysate has been beneficial on skin sensitivity by inhibiting the release of neurotransmitters such as substance P and improving skin barrier function. Other examples of lactic acid bacteria extracts can be cited, such as Streptococcus salivarius which increased the level of ceramides in the skin (Bifidobacterium longum lysate, a new ingredient for reactive skin. Audrey Guéniche et al., L'Oreal Research, 2009). Lactobacillus ferments are also already used in cosmetics but mainly as skin conditioning agents and antimicrobial preservatives. Document CN106860284 describes an anti-wrinkle composition consisting of the following numerous raw materials taken in combination for their effects, in particular antioxidants, in percentage by weight: 0.5% ginseng extract, 0.6% arbutin, 0,3% Glabridin, 0.2% Snail Protein Powder, 0.3% Licorice Flavonoid Liquid, 0.5% Tranmetracycline Acid, 0.6% Aloe Vera Extract, 0.8% Yeast Extract, 0.7% Honeysuckle Extract, 1.3% Cyanocobalamin, 1.2% Cellulase, 1.5% Lactobacillus Acidophilus, 1.2% Euphorbia Extract, 1.5% Salvia Miltiorrhiza Extract, Moxa Leaf Extract, 1.6% Atractylodes Extract, 1.3% Gynostemma Pentaphylla Extract, 1.5% Luffa Extract, 1.7% Gentian Extract, 1.1% Clove Bud Extract clove, 1.2% licorice root extract, 0.8% vitamin C, 0.9% chamomile extract, 1% centella asiatica extract, 0.9% angelica extract, 1% lily extract, 1.1% cornflower extract, 1.5% caprylic acid triglyceride, 1.2% frankincense and 71,4% ionized water. For example, we know the product LEUCIDAL® LIQUID SF which is a probiotic derived from the fermentation of Lactobacillus acidophillus known to hydrate the skin by reducing water loss from the skin. Furthermore, we know the use of thermal waters for their benefits on the skin. These waters naturally rich in mineral salts and trace elements have in particular moisturizing, soothing, remineralizing, anti-free radical, anti-inflammatory, healing, anti-irritant and decongestant properties, which gives them the ability to resolve many skin disorders (dryness, discomfort, irritations, etc.). However, no two thermal waters are alike. Indeed, depending on the source from which it is taken, thermal water does not have the same composition as other thermal water drawn from another source. For example, Jonzac thermal water is known and used for its regenerative properties (strengthens the skin barrier),anti-inflammatory (limits sensitivity and reactions linked to inflammation), soothing (calms the skin and limits redness) and moisturizing (reinforces the skin's natural hydration). TECHNICAL PROBLEM Considering the above, a problem that the invention seeks to solve is to provide a new biological solution to the protection of the skin's barrier function and its repair, for example following an external aggression. Unexpectedly, the Applicant has demonstrated the synergistic effects of a thermal water and a postbiotic, in particular making it possible to completely reduce the degradation of filaggrin and therefore to preserve the skin's barrier function, and also inducing an increase in the expression of collagen I. In addition, this protective or restorative action,allows to reduce by half the proliferative response and reflects a good anti-aging activity. TECHNICAL SOLUTION The solution to this problem posed has as its first object a cosmetic and / or dermatological composition characterized in that it comprises, in a physiologically acceptable medium, a thermal water whose mineral content is greater than 20 mg / L and a concentration of between 1% and 10% of an extract of Lactobacillus acidophilus by weight of the total weight of the composition. It also has as its object the uses of the composition according to the invention to protect, strengthen and / or repair the barrier function of the skin,to combat skin aging and to improve the skin microbiota. The invention and the advantages resulting therefrom will be better understood upon reading the description and the non-limiting embodiments which follow. BRIEF DESCRIPTION OF THE DRAWINGS The invention and the advantages resulting therefrom will be better understood upon reading the description and the non-limiting embodiments which follow, with regard to the appended figures in which: Figure 1 represents the epidermal hyper-proliferation after stripping at D8 by measuring the thickness of the epidermis on the different batches of human skin explants tested in the context of example 1. Figure 2 represents the effect of the products tested in the context of example 1 on the inhibition of epidermal hyper-proliferation induced by stripping at D8. Figure 3 illustrates the labeling of filaggrin in the stratum corneum,in the different batches of human skin explants tested in Example 1. Figure 4 represents the percentage of surface area occupied by filaggrin in the stratum corneum in the different batches of human skin explants tested in Example 1. Figure 5 illustrates the labeling of collagen I in the papillary dermis in the different batches of human skin explants tested in Example 1. Figure 6 represents the percentage of surface area occupied by collagen I in the papillary dermis in the different batches of human skin explants tested in Example 1. Figure 7 represents a summary table of the results obtained in terms of cell viability, epidermal thickness,expression of filaggrin and expression of collagen I in the different batches of human skin explants tested in the context of Example 1. Figure 8 represents a summary table of the comparative values ​​of expression of filaggrin and collagen I in the different batches of human skin explants tested in the context of Example 1. Figure 9 represents a linearized graph of the percentage (%) of DPPH reduction as a function of the trolox concentration (mg / L). Figure 10 represents the antiradical activity in percentage (%) of DPPH reduction of a combination of 2% Lactobacillus acidophilus extract + 0.5% Distilled water and of a combination of 2% Lactobacillus acidophilus extract + 0.5% Jonzac thermal spring water. DESCRIPTION OF EMBODIMENTS The invention relates to a cosmetic and / or dermatological composition characterized in that it comprises, in a physiologically acceptable medium,a thermal water with a mineral content greater than 20 mg / L and a concentration of between 1% and 10% of an extract of Lactobacillus acidophilus by weight of the total weight of the composition. A physiologically acceptable medium means a medium suitable for use in contact with human and animal cells, in particular epidermal cells, without toxicity, irritation, undue allergic response and the like, and proportionate to a reasonable benefit / risk ratio. Such a physiologically acceptable medium may comprise excipients known and used in the cosmetic and dermatological fields. A person skilled in the art will take care to choose the physiologically acceptable medium so that it does not harm the advantageous properties of the compositions according to the invention. Thermal water means a naturally mineralized spring water, with a mineral content greater than 20 mg / L, preferably greater than 150 mg / L,preferably greater than 1000 mg / L, even more preferably greater than 5000 mg / L, and even more preferably greater than 7000 mg / L. Preferably, the thermal water is chosen from thermal water from Jonzac, Rochefort, Avène, La Roche Posay, Uriage, Saint Gervais, Gamarde, Fumades, Cauteret, Evaux, Vichy, more preferably thermal water from Jonzac and Rochefort, even more preferably thermal water from Jonzac. The thermal water preferably used is a polymetallic sulphated thermal water mixed with calcium, sodium chloride, magnesium, hypermineralized with a mineral content of at least 5000 mg / L, for example of the order of 7000 mg / L, comprising essentially calcium and magnesium, as well as silicon, strontium, boron, lithium and manganese, hypotonic, practically isotonic. Preferably,The thermal water used is Jonzac thermal water. Jonzac thermal water is more specifically a sulfurous, mixed sulfate, sodium chloride type water, rich in trace elements and hypermineralized with a mineral content greater than 6500 mg / L, for example around 7000 mg / L. Among the minerals, we mainly find calcium (Ca2+), magnesium (Mg2+) and also strontium (Sr). Jonzac thermal water is a hyperthermal water (emergence temperature at 62°C), bacteriologically pure and isotonic, and with a pH of 6.95. The composition according to the invention preferably comprises thermal water at a concentration of between 0.5% and 99.5% by weight of the total weight of the composition, for example 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, preferably between 5% and 50%, more preferably between 10% and 35%,even more preferably 20% or 30%. The composition according to the invention comprises a postbiotic, i.e. a preparation of inanimate microorganisms and / or their components which may confer in particular a health benefit. A postbiotic is distinguished from a probiotic insofar as the latter is inactivated following the fermentation process. Thus, the postbiotic may correspond to the microbial cell in its entirety or only to certain components thereof, or even to metabolites. The composition according to the invention comprises a cutaneous postbiotic, namely an extract of Lactobacillus acidophilus. Lactobacillus acidophilus is a Gram+, non-spore-forming, rod-shaped lactic acid bacterium with rounded ends. It is non-motile and appears isolated, in pairs or in short chains. It is a bacterium which grows at an optimal temperature between 35°C and 40°C,although it is capable of growing up to 45°C. Its optimal growth pH is between 5.5 and 6. Most strains are aerotolerant, but growth remains optimal under microaerobic or even anaerobic conditions. The Lactobacillus acidophilus extract preferably used is a Lactobacillus acidophilus cell lysate originating from the fermentation of Lactobacillus acidophilus. According to an advantageous embodiment, the Lactobacillus acidophilus cell lysate originates from the fermentation of Lactobacillus acidophilus in a culture medium comprising ammonium sulfate, magnesium sulfate,disodium phosphate (mineral) and a natural yeast autolysate followed by controlled lysis in the presence of a lysozyme derived from Papaya (Carica papaya) and after filtration, removal of the biomass and recovery of said cell lysate. The Lactobacillus acidophilus extract used is, for example, an extract of the strain Lactobacillus acidophilus ATCC 314. The composition according to the invention preferably comprises the Lactobacillus acidophilus extract at a concentration of between more than 1.5% and 10% by weight of the total weight of the composition, more preferably between 2% and 10% by weight of the total weight of the composition, for example 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, more preferably still between 2% and 5%, even more preferably 2%. Preferably, the composition according to the invention further comprises hyaluronic acid and its derivatives, a hydroxyproline and its derivatives such as dipalmitoyl hydroxyproline, a hydrolyzed lupin protein,an algae extract such as an aqueous extract of brown algae such as fucus and laminaria rich in proteins, an oily extract of green algae such as alaria rich in omega 3, 6 and 9 or chlorella, or an extract of red algae such as Jania rubens, oligosaccharides (prebiotics), zinc PCA, mallow or chamomile floral water, witch hazel extract, Centella asiatica extract, aloe vera extract / juice, ceramides such as N-oleoyl-phytosphingosine (or ceramide NP), phytosphingosine, sodium carrageenan, dextrin, phytic acid, tocopherol, buckwheat wax, shea butter, castor oil, vegetable squalane, glycerin, taken alone or in combination. More preferably, the composition according to the invention further comprises hyaluronic acid and its derivatives, a mallow floral water, an aloe vera extract / juice, an extract of Centella asiatica, an extract of red algae such as Jania rubens,sodium carrageenan, phytosphingosine, dextrin, phytic acid, tocopherol, buckwheat wax, shea butter, castor oil, vegetable squalane, glycerin, taken alone or in combination. Preferably, the compositions according to the invention are in a form suitable for topical administration. As illustrative examples of topical formulations according to the invention, mention may be made of an optionally tinted cream, an oil-in-water (O / W) emulsion, a water-in-oil (W / O) emulsion, a gel, a gel-cream, a water, a body or lip balm, an ointment, a serum, an eye and lip contour product, a micellar water, a mask, or even a cleansing milk. Another subject of the invention relates to a composition according to the invention, used to protect,strengthen and / or repair the skin barrier function and reduce feelings of discomfort on sensitive to reactive skin or even atopic skin. By protecting, strengthening and / or repairing the skin barrier function is meant, for example, improving skin hydration, i.e., all improvements in changes in the external appearance of the skin due to dehydration, such as, for example, dryness, tightness, and soothing feelings of discomfort and reducing reactivity so as to help prevent the appearance of visible skin reactions, in particular on sensitive to reactive skin or even atopic skin. The composition according to the invention is also used to combat skin aging, in particular for normal to sensitive skin, by strengthening the firmness of the skin. By signs of skin aging is meant all changes in the external appearance of the skin due to aging, such as, for example,wrinkles and fine lines, cracks, bags under the eyes, dark circles, sagging, loss of elasticity, firmness and / or tone of the skin, but also any internal changes to the skin which do not systematically result in a modified external appearance such as, for example, thinning of the skin, or any internal degradation of the skin. The composition according to the invention is also used to improve the skin microbiota. As illustrative examples, the composition according to the invention can make the skin less prone and sensitive to the penetration of allergens, bacteria, viruses and inflammation and thus prevent the development of skin pathologies such as Atopic Dermatitis,to prevent premature aging and the appearance of wrinkles and to improve skin hydration. EXAMPLES The present invention will now be illustrated by means of the following examples: Example 1: Evaluation of the activity of Jonzac Thermal Spring Water, Lactobacillus acidophilus and their association on human skin explants This study, divided into two phases, aims to evaluate the anti-aging and epidermal barrier strengthening actions of two products, as well as their synergy on ex vivo human skin explants. The first ex vivo phase makes it possible to reproduce an application of the product on the skin. The second histological phase makes it possible to evaluate the evolution of the biological parameters by staining and immunolabeling. The activity is evaluated by: - ​​an evaluation of cell viability after staining with Masson's trichrome with a measurement of the thickness of the epidermis, - immunolabeling of filaggrin,and - immunolabeling of collagen I. The products tested are as follows: - P1 = Jonzac thermal spring water; and - P2 = Lactobacillus acidophilus extract. In the following examples, it is understood that P1 designates Jonzac thermal spring water and P2 designates an extract of Lactobacillus acidophilus. On D0, products P1 and P2 are diluted in sterile distilled water as follows: - Batch P1 = P1 at 20%; - Batch P2 = P2 at 2%; and - Batch P3 = P1 at 20% + P2 at 2%. The prepared products were stored at 4°C during the ex vivo phase of the study. Phase 1: Explant Preparation For the ex vivo phase of the study, 18 human skin explants measuring 12 ± 1 mm in diameter, 12 of which had undergone 10 strippings (Lot S and S'x'), were prepared from an abdominoplasty of a 69-year-old Caucasian woman (reference P2660-AB69) of phototype II. Stripping is a model of mechanical skin irritation which consists of progressively detaching the superficial part of the epidermis,the stratum corneum. Skin irritation is achieved by successively applying and removing an adhesive strip on the same skin area. The explants were kept alive in a suitable environment at 37°C in a humid atmosphere, enriched with 5% CO2. Aggression of the epidermal barrier On D0, just before application of the products, the explants of batches S and S'x' were stripped 10 times using adhesive tape. Distribution of explants The explants were divided into 6 batches as follows: Table 1: Batch Designation / Treatment Number of explants Stop T0 Plastic surgery control 3 D0 T Untreated control 3 D8 S Stripped control: 3 D8 x10 strippings SP1 Strippings x10 + P1 at 20% 3 D8 SP2 Strippings x10 + P2 at 2% 3 D8 SP3 Strippings x10 + Mixture P1 3 D8 at 20% + P2 at 2% Application of products On D0, D2, D3 and D6, the tested products P1, P2 and P3 were applied topically,at a rate of 2μL per 1cm² explant, i.e. approximately 2mg / cm², and spread using a small spatula. The explants in the control batches received no treatment, except for renewal of the medium. Half of the medium was renewed (1mL / well) on D2, D3 and D6. Samples On D0, the 3 explants from batch T0 were collected. Each sample was cut in half, one half was frozen at -80°C at the Optimal Cutting Temperature (OCT), the other half was fixed in formalin. On D8, 3 explants from each batch were collected and treated in the same way as on D0 described above. Phase 2: Histological treatments The samples described above were conditioned for 24 hours in buffered formalin, then were dehydrated and impregnated in paraffin using a Leica PEARL dehydration machine. They were block-embedded using a Leica EG 1160 embedding station. 5 μm sections were made using a Minot microtome,Leica RM 2125 and mounted on Superfrost® histological glass slides. Microscopic observations were carried out using optical microscopy, using a Leica DMLB, Olympus BX43 or Olympus BX63 microscope. Images were taken with an Olympus DP72 or DP74 camera and cellSens software. 1. Cell viability Cell viability of epidermal and dermal structures was assessed on paraffin sections after staining with Masson trichrome Goldner variant. It was assessed by microscopic examination. All batches are affected by this manipulation. 2. Measurement of epidermal thickness On each image resulting from Masson trichrome staining, the thickness of the epidermis was measured at several points using the measurement module of the Olympus CellSens software. 3 thickness measurements were carried out for each image,corresponding to 27 measurements per batch for all batches. All batches are affected by this manipulation. 3. Filaggrin immunostaining Filaggrin immunostaining was performed on paraffin sections with an anti-filaggrin monoclonal antibody (Santa Cruz, sc-66192, clone AKH1) diluted 1 / 100 in PBS-BSA 0.3%, for 1 hour at room temperature, and revealed in AlexaFluor AF488 (Life Technologies, ref. A11008). The nuclei were counterstained with propidium iodide. Immunostaining was assessed by microscopic observation. All batches are affected by this manipulation. 4. Immunostaining of collagen I Collagen I was labeled on frozen sections with a polyclonal anti-collagen I antibody (Abcam, ab138492-1001) diluted 1 / 800 in PBS-BSA 0.3%, overnight at room temperature, and revealed in AlexaFluor AF488 (Lifetechnologies,ref. A11008). The nuclei were counterstained with propidium iodide. Immunostaining was assessed by microscopic observation and semi-quantified by image analysis. All batches are affected by this manipulation. Results 1. Cell viability The cell viability of all batches is shown in the table below: Table 2: Batch Cell viability Epidermis Dermis T0 BB TJ8 AB B SJ8 AB B SP1J8 AB B SP2J8 AB B SP3J8 AB B Morphology legend: B=Good, AB= Fairly good, TLA= Very slightly altered, LA= Slightly altered, MA= Moderately altered, ANA= Fairly markedly altered, NA= Markedly altered, TNA= Very markedly altered At D0, on the control batch (T0), cell viability is good in the epidermis and papillary dermis. At D8, on the control batch (TJ8),Cell viability is quite good in the epidermis and good in the papillary dermis. Aggression of the epidermal barrier via the performance of 10 strippings does not induce any modification of cell viability compared to the control batch TJ8. Effect of the product on cell viability, compared to batch SJ8: - Product P1 does not induce any modification. - Product P2 does not induce any modification. - Product P3 does not induce any modification. Effect of the product on cell viability, compared to batch SP1J8: - Product P3 does not induce any modification. Effect of the product on cell viability, compared to batch SP2J8: - Product P3 does not induce any modification. 2. Measurement of epidermal hyperproliferation 2.1 Thickness of the epidermis When the skin is weakened by skin stripping,several major phenomena are observed: - an alteration of the barrier function which results in the reduction of certain markers of the stratum corneum including filaggrin or lipids; - epidermal hyper-proliferation put in place by the skin in order to restore an optimal barrier function; - an increased secretion of certain pro-inflammatory cytokines including IL-1 alpha. When a product is tested on an ex vivo model of human skin weakened by stripping, its effectiveness is evaluated on several parameters, namely its capacity to reduce epidermal hyper-proliferation or its activity on the expression of markers of the barrier function. The analysis of epidermal hyperproliferation after stripping via the measurement of epidermal thickness on all batches is shown in the table below and illustrated by Figure 1. Table 3: Epidermal thickness (μm) T0 TJ8 SJ8 SP1J8 SP2J8 SP3J8 Average 27.2 51.1 72.9 73.0 60.8 64.5 Standard deviation 3.5 5.8 7.3 7.8 6.5 7.9 At D0, on the control batch T0, the epidermis measures on average 27.2 μm. At D8, on the control batch TJ8, the epidermis measures on average 51.1 μm. The aggression of the epidermal barrier via the realization of 10 strippings induces epidermal hyper-proliferation which results in a significant increase in the thickness of the epidermis of 43%** compared to the control batch TJ8. Effect of the application of the products on the thickness of the epidermis, compared to the control batch SJ8: - Product P1 does not induce any modification; - Product P2 induces a significant decrease of 17%** therefore it reduces the stripping-induced epidermal hyper-proliferation; - Product P3 induces a significant decrease of 12%**, thus reducing stripping-induced epidermal hyperproliferation. Effect of product application on epidermal thickness,compared to batch SP1J8: - Product P3 induces a significant decrease of 12%**, therefore it reduces stripping-induced epidermal hyperproliferation. Effect of product application on epidermal thickness, compared to batch SP2J8: - Product P3 induces a significant increase of 6%#. Legend: - Non-significant: ns; - Significant: # for p<0.1 (90%); * for p<0.05 (95%); ** for p<0.01 (99%). By removing the upper part of the stratum corneum by stripping, the skin barrier function was impaired. Following stripping (aggression), the epidermis will accelerate the terminal differentiation of keratinocytes into corneocytes to reform the stratum corneum. To compensate for this loss of keratinocytes,there is an activation of proliferation at the level of the basal layer of the epidermis (cf. thickness of the epidermis of the stripped control which increases). Product P1 (ETJ 20%) does not modify the proliferative response (thickness of the epidermis) following the loss of barrier function. Product P2 (Lactobacillus acidophilus extract at 2%) allows the proliferative response to be reduced by half. This therefore means that P2 has a protective action against this response. The combination of the two products allows the proliferative response to be reduced by half and therefore also has a protective action. 2.2 Inhibition of hyper-proliferation The percentage of inhibition of epidermal hyper-proliferation induced by stripping at D8 for the batches concerned is shown in the table below and illustrated by Figure 2. Table 4: % inhibition of epidermal hyper-proliferation SP1D8 SP2D8 SP3D8 0% 56% 39% Effect of product application on epidermal hyper-proliferation,compared to the control batch SJ8: - Product P1 does not induce any modification. - Product P2 induces a significant inhibition of 56%**. - Product P3 induces a significant inhibition of 39%**. Legend: - Non-significant: ns - Significant: # for p<0.1 (90%); * for p<0.05 (95%); ** for p<0.01 (99%). 3. Filaggrin Filaggrin labeling in the stratum corneum, on all batches is illustrated in Figure 3. At D0, on the control batch T0, filaggrin labeling is moderate to fairly clear in the stratum corneum. At D8, on the control batch TJ8, filaggrin expression is moderate to fairly clear in the stratum corneum. The aggression of the epidermal barrier via the realization of 10 strippings (SJ8) induces a moderate decrease in the expression of filaggrin compared to the control batch TJ8. Effect of the application of the products on the expression of filaggrin,compared to the control batch SJ8: - Product P1 induces a slight increase; - Product P2 induces a slight increase; - Product P3 induces a moderate increase. Effect of the application of the products on the expression of filaggrin, compared to batch SP1J8: - Product P3 induces a slight increase. Effect of the application of the products on the expression of filaggrin, compared to batch SP2J8: - Product P3 induces a slight increase. Image analysis of filaggrin For each batch of explants,The percentage of the area of ​​the region of interest covered by the labeling (percentage of labeled area) is determined by image analysis. Comparisons of labeled areas The percentage of labeled area (%area) for each treatment is compared to the untreated condition. Image analysis of filaggrin was performed using cellSens software (Olympus). Area analyzed per image: ROI including the stratum corneum. Number of images analyzed per batch: 9. Statistical test: Student's t-test. Batches analyzed: T0, [T, S, SP1, SP2, SP3] to D8. The percentage of surface area occupied by filaggrin in the stratum corneum is shown in the table below and illustrated in Figure 4. Table 5: Filaggrin (%surface area) T0 TJ8 SJ8 SP1J8 SP2J8 SP3J8 Mean 69.3 91.5 65.4 83.1 77.4 88.6 Standard deviation 8.8 5.1 14.9 12.0 12.9 7.4 At D0, on the control batch T0, filaggrin represents 69.3% of the surface area of ​​the stratum corneum. At D8, on the control batch TJ8,Filaggrin represents 91.5% of the surface of the stratum corneum. The aggression of the epidermal barrier via the realization of 10 strippings induces a significant decrease of 29%** of the expression of filaggrin compared to the control batch TJ8. Effect of the application of the products on the expression of filaggrin, compared to the control batch SJ8: - Product P1 induces a significant increase of 27%*; - Product P2 induces a significant increase of 18%#; - Product P3 induces a significant increase of 35%**. Effect of the application of the product on the expression of filaggrin, compared to batch SP1J8: - Product P3 induces a non-significant increase of 7%ns. Effect of the application of the product on the expression of filaggrin,compared to batch SP2J8: - Product P3 induces a significant increase of 14%*. Legend: - Non-significant: ns; - Significant: # for p<0.1 (90%); * for p<0.05 (95%); ** for p<0.01 (99%). 4. Collagen I The labeling of collagen I in the papillary dermis, on all batches is illustrated in Figure 5. At D0, on the control batch T0, the labeling of collagen I is fairly clear to clear in the papillary dermis. At D8, on the control batch TJ8, the expression of collagen I is moderate to fairly clear in the papillary dermis. The aggression of the epidermal barrier via the performance of 10 strippings does not induce any modification of the expression of collagen I in the control batch TJ8. Effect of the application of the products on the expression of collagen I,compared to the control batch SJ8: - Product P1 induces a slight increase; - Product P2 does not induce any modification; - Product P3 induces a slight increase. Effect of the application of the products on the expression of collagen I, compared to batch SP1J8: - Product P3 does not induce any modification. Effect of the application of the products on the expression of collagen I, compared to batch SP2J8: - Product P3 induces a slight increase. Image analysis of collagen I For each batch of explants,The percentage of the area of ​​the region of interest covered by the labeling (percentage of labeled area) is determined by image analysis. Comparisons of labeled areas The percentage of labeled area (%area) for each treatment is compared to the untreated condition. Image analysis of collagen I was performed using cellSens software (Olympus). Area analyzed per image: ROI including the papillary dermis. Number of images analyzed per batch: 9. Statistical test: Student's t-test. Batches analyzed: T0, [T, S, SP1, SP2, SP3] to D8. The percentage of surface area occupied by collagen I in the papillary dermis is shown below is shown in the table below and illustrated in Figure 6. Table 6: Collagen I (%surface) T0 TJ8 SJ8 SP1J8 SP2J8 SP3J8 Mean 88.0 69.6 70.6 76.5 71.8 78.3 Standard deviation 4.0 7.5 8.0 6.0 6.3 7.9 At D8, on the control batch TJ8,Collagen I represents 69.6% of the surface of the papillary dermis. Aggression of the epidermal barrier via the performance of 10 strippings induces a non-significant increase of 1%ns in the expression of collagen I compared to the control batch TJ8. Effect of the application of the products on the expression of collagen I, compared to the control batch SJ8: - Product P1 induces a significant increase of 8%#; - Product P2 induces a non-significant increase of 2%ns; - Product P3 induces a significant increase of 11%#. Effect of the application of the product on the expression of collagen I, compared to batch SP1J8: - Product P3 induces a non-significant increase of 2%ns. Effect of the application of the product on the expression of collagen I,compared to batch SP2J8: - Product P3 induces a significant increase of 9%#. Legend: - Non-significant: ns; - Significant: # for p<0.1 (90%); * for p<0.05 (95%); ** for p<0.01 (99%). Conclusion The summary of the results obtained is illustrated in Figures 7 and 8. According to the experimental conditions described above and compared to the control batch T or the stripped batch S at D8: After 8 days of treatment, all the products tested are well tolerated by the ex vivo human skin explants. The product “Active 1: Jonzac Thermal Spring Water” at 20% (P1) induces a slight increase in the expression of filaggrin after strippings, highlighting a skin barrier strengthening activity. It also induces a slight increase in the expression of collagen I, reflecting an anti-aging activity. The product “Active 2: Lactobacillus acidophilus” at 2% (P2) induces a slight increase in the expression of filaggrin after stripping,associated with a decrease in epidermal thickness (-17%**) highlighting good skin barrier strengthening activity. The combination of the products “Active 1: Jonzac Thermal Spring Water” at 20% (P1) and “Active 2: Lactobacillus acidophilus” at 2% (P2) induces an increase in filaggrin expression after strippings, associated with a decrease in epidermal thickness (-12%**) highlighting good skin barrier strengthening activity. This combination also induces a slight increase in collagen I expression reflecting good anti-aging activity. The combination of the products “Active 1: Jonzac Thermal Spring Water” at 20% (P1) and “Active 2: Lactobacillus acidophilus” at 2% (P2) has an additional effect on the reinforcement of the barrier effect, the expression of filaggrin and collagen I. Interpretation of the results regarding the thickness of the epidermis: by removing the upper part of the stratum corneum by stripping,the skin's barrier function has been impaired. In response to this loss of barrier function, filaggrin is degraded into amino acids that will strengthen the natural moisturizing factors (NMF Natural moisturizing Factor) and bind a large quantity of water. At the same time, the epidermis will accelerate the terminal differentiation of keratinocytes into corneocytes to reform the stratum corneum. To compensate for this loss of keratinocytes, there is an activation of proliferation at the level of the basal layer of the epidermis. Product P1 reduces the degradation of filaggrin by half, it partially compensates (or repairs) the barrier function of the skin. In addition, it does not modify the proliferative response (thickness of the epidermis) following the loss of barrier function. Product P2 reduces the degradation of filaggrin by half, it partially compensates (or repairs) the barrier function of the skin. In addition, this protective or restorative action,reduces the proliferative response by half. The combination of the two products completely reduces the degradation of filaggrin and therefore preserves the skin's barrier function. In addition, this protective or restorative action reduces the proliferative response by half. Example 2: W / O Emulsion Ingredients % in the formula (w / w) Emulsifier 2-5% Consistency factors 0.5-5% Emollients 10-20% Antioxidant 0.01-0.5% JONZAC thermal spring water 15-50% Organic mallow floral water (or witch hazel) 5-25% or organic aloe vera extract 0.01-1% Moisturizing agent (glycerin, propanediol, pentylene 1-5% glycol) Lactobacillus acidophilus extract 2-10% Other active ingredients (chosen from phytosphingosine, dextrin, 0.05-5% bisabolol, hyaluronic acid, buckwheat wax, Centella asiatica, Jania rubens extract, sodium carrageenan,…) Perfume 0-0.8% Preservatives and antimicrobial agents 0.001-3% pH adjuster 0.010.5% Demineralized water qsp 100% Example 3: O / W emulsion Ingredients % in the formula (w / w) Emulsifier 2-5% Co-emulsifiers 0.1-3% Consistency factors 0.5-5% Emollients 5-25% Antioxidant 0.01-0.5% JONZAC thermal spring water 20-50% Organic mallow floral water (or witch hazel) 5-25% or organic aloe vera extract 0.01-1% Moisturizing agent (glycerin, propanediol, pentylene 1-10% glycol) Gelling agent 0.1-2% Chelating agent 0.05-0.5% Lactobacillus acidophilus extract 2-10% Other active ingredients (phytosphingosine, dextrin, bisabolol, 0.05-5% hyaluronic acid, buckwheat wax, Centella asiatica, Jania rubens extract, sodium carrageenan,…) Perfume 0-0.8% Preservatives and antimicrobial agents 0.001-3% pH adjuster 0.01-0.5% Demineralized water qsp 100% Example 4: Micellar water Ingredients % in the formula (w / w) JONZAC thermal spring water 15-50% Organic mallow floral water (or witch hazel) 5-25% or organic aloe vera extract 0.01-1% Moisturizing agent (glycerin, propanediol, pentylene 1-10% glycol) Lactobacillus acidophilus extract 2-10% Other active ingredients (phytosphingosine, dextrin, bisabolol, 0.05-5% hyaluronic acid, buckwheat wax, Centella asiatica, Jania rubens extract, sodium carrageenan, …) Perfume 0.01-0.8% Preservatives and antimicrobial agents antimicrobials 0.001-3% Solubilizer 0-3% pH adjuster 0.01-0.5% Demineralized water qsp 100% Example 5: Care gel / Makeup remover jelly Ingredients % in the formula (w / w) JONZAC thermal spring water 5-50% Organic mallow floral water (or witch hazel) 5-25% or organic aloe vera extract 0.01-1% Moisturizing agent (glycerin, propanediol,pentylene 1-15% glycol) Gelling agent 0.1-4% Lactobacillus acidophilus extract 2-10% Other active ingredients (phytosphingosine, dextrin, bisabolol, 0.05-5% hyaluronic acid, buckwheat wax, Centella asiatica, Jania rubens extract, sodium carrageenan, …) Perfume 0-0.8% Preservatives and antimicrobial agents 0.001-3% Solubilizer 0-3% pH adjuster 0.01-0.5% Demineralized water qsp 100% Example 6: Gel-cream Ingredients % in the formula (w / w) Emulsifier 0.5-2% Consistency factors 0.5-5% Emollients 2-25% Antioxidant 0.01-0.5% JONZAC thermal spring water 0.05-50% Organic mallow floral water (or witch hazel) 5-25% or organic aloe vera extract 0.01-1% Moisturizing agent (glycerin, propanediol, 1-10% pentylene glycol) Gelling agent 0.2-2% Emulsifying gelling agent 0.05-2% Chelating agent 0.1-0.3% Lactobacillus acidophilus extract 2-10% Other active ingredients (phytosphingosine, dextrin, bisabolol, 0.05-5% hyaluronic acid, buckwheat wax, Centella asiatica, Jania rubens extract, sodium carrageenan,…) Perfume 0.05-0.8% Preservatives and antimicrobial agents 0.001-3% pH adjuster 0.01-0.5% Demineralized water qsp 100% Example 7: Evaluation of the antiradical activity of a combination of active ingredients by the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) test The purpose of this study is to evaluate the antiradical potential of the combination of a Lactobacillus acidophilus extract and Jonzac Thermal Spring Water. The antiradical activity of the product studied is assessed by measuring the reduction rate of the DPPH radical in the presence of said product. The reduction rate of the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) radical is evaluated by the variation in absorbance (abs) of a DPPH solution in the presence of the tested product. DPPH is a stable colored radical. Reduction of the DPPH radical by a 1-1 atom donor leads to the formation of colorless 1,1-diphenyl-2-picrylhydrazine (DPPH-H). The reaction mixture then changes from dark purple to light purple,or even colorless for the most antioxidant products. The absorbance of the solutions is measured at 517 nm after 24 hours of incubation in the presence of the product tested in a DPPH solution (0.126 mM in ethanol). The percentage of DPPH reduction is calculated according to the following formula: % DPPH Reduction = ((Reference Abs – Test Element Abs) / Reference Abs)*100,with: Abs reference = absorbance of the solvent; and Abs test element = absorbance of the test element. The concentration of the test element giving a 50% reduction in DPPH is determined (IC50). It reflects its anti-radical activity. The activity of the test element is compared to that of a known anti-radical active ingredient: Trolox. Four products are tested: - the combination of 2% Lactobacillus acidophilus extract + 0.5% distilled water; - the combination of 2% Lactobacillus acidophilus extract + 0.5% Jonzac Thermal Spring Water; - Jonzac Thermal Spring Water alone; and - Trolox (positive control). The DPPH test results thus obtained are presented in the table below and the linearized graph of % DPPH Reduction as a function of trolox concentration is shown in Figure 9. Table 7: DPPH test results with trolox Concentration Reference 0.625 1.25 2.5 3.75 5 (mg / L) % Reduction / 4.969 9.078 21.548 36.550 48.256 DPPH The IC50 of trolox,effective concentration which corresponds to a 50% reduction in DPPH activity, is determined using the equation of the line (ax+b) shown in Figure 9, according to the following formula: IC50 (mg / L) = (50-b) / a. We thus find the value: IC50 trolox = 5.168 mg / L. This control validates the test. For the test with Jonzac thermal spring water, the following final concentrations (% w / v] were analyzed. The DPPH test results thus obtained are presented in the table below. Table 8: Results of the DPPH test with Jonzac thermal spring water Concentration (% w / v) Reference 2.5 2 1.5 1 0.5 % DPPH reduction / -2.676 -2.793 -1.784 -0.698 -1.474 For the test with the combination 2% Lactobacillus acidophilus extract + 0.5% Jonzac thermal spring water,The test element was tested at the following final concentrations (% w / v). The results obtained are presented in the table below. Jonzac Thermal Spring Water alone does not exhibit any antiradical power. Table 9: Results of the DPPH test with the combination 2% Lactobacillus acidophilus extract + 0.5% Jonzac Thermal Spring Water Concentration Reference 2% Lactobacillus acidophilus extract + (% w / v) 0.5% Jonzac Thermal Spring Water % Reduction / 64.885 DPPH For comparison of the antioxidant power, Jonzac Thermal Spring Water was replaced by distilled water, in the following final concentrations (% w / v). The combination 2% Lactobacillus acidophilus extract + 0.5% distilled water was tested at DPPH,and the results thus obtained are presented in the table below. Table 10: Results of the DPPH test with the combination 2% Lactobacillus acidophilus extract + 0.5% distilled water Concentration Reference 2% Lactobacillus acidophilus extract + (%w / v) 0.5% Distilled water % Reduction / 61.363 DPPH The antiradical power of the combination of Jonzac Thermal Spring Water added to the Lactobacillus acidophilus extract is evaluated by comparison with the antiradical power of the combination of distilled water added to the Lactobacillus acidophilus extract. The results are presented in Figure 10. A statistical test (Student t-test) is carried out to evaluate the significance of the difference in antiradical power between the two combinations. The Student t-test gives the probability for two batches to be significantly different. The difference between two batches is significant if p<0.1(#),either a probability of 90% for two batches to be significantly different or p<0.05(*), or a probability of 95% for two batches to be significantly different or p<0.01(**), or a probability of 99% for two batches to be significantly different. The results thus obtained show that the combination of an extract of Lactobacillus acidophilus in combination with distilled water, at the concentrations tested, highlights a trapping effect against the DPPH radical, with a percentage of DPPH Reduction of 61.363%, greater than 50%. By comparison, the combination of an extract of Lactobacillus acidophilus in combination with Jonzac Thermal Spring Water (instead of distilled water), at the concentrations tested, highlights a trapping effect on the DPPH radical, with a percentage of DPPH Reduction of 64.885%, greater than 50%. The Student t-test obtained gives a value of p=0.013, or p<0.05(*) which indicates a 95% probability that the two batches thus tested are significantly different. In conclusion, under the same conditions, compared to distilled water, the addition of Jonzac Thermal Spring Water (used at a concentration of at least 0.5%), significantly improves the antioxidant effect of Lactobacillus acidophilus extract (used at a concentration of at least 2%). A significant synergistic effect of +5.67% (p<0.05) is observed.

Claims

CLAIMS 1. Cosmetic and / or dermatological composition characterized in that it comprises, in a physiologically acceptable medium, a thermal water whose mineral content is greater than 20 mg / L and a concentration of between 1% and 10% of an extract of Lactobacillus acidophilus by weight of the total weight of the composition.

2. Composition according to claim 1, characterized in that the thermal water is chosen from thermal water from Jonzac, Rochefort, Avène, La Roche Posay, Uriage, Saint Gervais, Gamarde, Fumades, Cauteret, Evaux, Vichy, preferentially thermal water from Jonzac.

3. Composition according to claim 1 or 2, characterized in that it comprises thermal water at a concentration of between 0.5% and 99.5% by weight of the total weight of the composition, preferably between 5% and 50%, more preferably between 10% and 35%, even more preferably 20% or 30%. 4.Composition according to one of the preceding claims, characterized in that the Lactobacillus acidophilus extract is a cell lysate of Lactobacillus acidophilus originating from the fermentation of Lactobacillus acidophilus.

5. Composition according to one of the preceding claims, characterized in that it comprises the Lactobacillus acidophilus extract at a concentration of between more than 1.5% and 10% by weight of the total weight of the composition, preferably between 2% and 10%, more preferably between 2% and 5%, even more preferably 2% by weight of the total weight of the composition. 6.Composition according to one of the preceding claims, characterized in that it further comprises hyaluronic acid and its derivatives, a hydroxyproline and its derivatives such as dipalmitoyl hydroxyproline, a hydrolyzed lupin protein, an algae extract such as an aqueous extract of brown algae such as fucus and laminaria rich in proteins, an oily extract of green algae such as alaria rich in omega 3, 6 and 9 or chlorella, or an extract of red algae such as Jania rubens, oligosaccharides (prebiotics), zinc PCA, a mallow floral water or. of chamomile, a witch hazel extract, a Centella asiatica extract, an aloe vera extract / juice, ceramides such as N-oleoyl-phytosphingosine (or ceramide NP), a phytosphingosine, sodium carrageenan, a dextrin, phytic acid, tocopherol, buckwheat wax, shea butter, castor oil, vegetable squalane, glycerin, taken alone or in combination.

7. Composition according to one of the preceding claims, characterized in that it is in a form suitable for topical administration.

8. Composition according to one of claims 1 to 7 for its use for protecting, strengthening and / or repairing the barrier function of the skin and reducing feelings of discomfort on sensitive to reactive skin or even atopic skin.

9. Composition according to one of claims 1 to 7, for its use in combating skin aging. 10.Composition according to one of claims 1 to 7, for its use in improving the skin microbiota.

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