Agrochemical combinations, compositions and method of use thereof

Abstract

The present invention relates to an agrochemical combination and compositions of insecticides and a sea-weed algal extract. The said combinations and compositions control pest infestation in crops, improve crop yield and crop health and growth.

Description

[0001] AGROCHEMICAL COMBINATIONS, COMPOSITIONS AND METHOD OF USE THEREOF

[0002] FIELD OF INVENTION

[0003] The present invention relates to agrochemical combinationsand compositions. Particularly, the present invention relates to agrochemical combinations and compositions comprising insecticide(s) and a sea-weed algal extract. The said combinations and compositions control pest infestation in crops, improve crop yield and crop growth.

[0004] BACKGROUND OF INVENTION

[0005] Maintaining crop quality is a greater concern for consumers, crop quality indicators can be used as a measure of crop health and yield potentiality. Crop damage from pathogens and pests is a worldwide problem. Agriculture crop productivity has been severely affected by various pests. The protection of crops and its produce from insect pest damage is essential in agriculture produce enhancement. Chemical control by use of various chemicals and formulations is an important tool in agriculture for the prevention and control of pests. Insecticides of many types and groups are reported in literature and a large number are in use, commercially, for control of pests in agriculture.

[0006] However, the excessive use of chemicals in agriculture has led to a multitude of effects, including entry of toxic pesticide by-products into the food chain, increased residues in plants, insect resistance, and contamination of soil, water, and air. Disturbances in the food chain due to the accumulation of pesticide residues have a significant impact on ecology. Biological-based pesticides, or biopesticides, have been adopted in agriculture as an alternative solution to chemical pesticides.

[0007] Pyrethroids and pyrethrins are a predominant class of insecticides against pests. Pyrethroids are synthetic derivates of pyrethrins, which are natural organic insecticides procured from the flowers of Chrysanthemum cinerariaefolium and C. coccineum. Pyrethroids are classified into two groups — class I and class II — based on their toxicity and physical properties. Class I pyrethroids have a basic structure of cyclopropane carboxylic ester. These include allethrin, bifenthrin, permethrin, phenothrin, resmethrin, tefluthrin, and tetramethrin. Class II pyrethroids have a cyano group and cause choreoathetosis and salivation. These include cyfluthrin, cyhalothrin, cypermethrin, deltamethrin, fenvalerate, fenpropathrin, flucythrinate, flumethrin, fluvalinate, and tralomethrin. The prominent site of insecticidal action of pyrethroids is the voltage-sensitive sodium channels.

[0008] Avermectins are a family of 16-membered macrocyclic lactone natural product homologues produced by the soil microorganism Streptomyces avermitilis MA-4680 (NR.R.L 8165). The avermectins are used in agriculture / horticulture to control insects on many crops and ornamentals. The avermectins exert therapeutic effects by binding to glutamate-gated chloride channels, causing a flaccid paralysis and insect death.

[0009] Insecticide resistance is a heritable change in the susceptibility of a pest population to a particular insecticide, or group. Insects that repeatedly fail to be adequately controlled by the registered rate of an insecticide are said to be insecticide resistant. Where insecticide resistance levels are high, pest populations may be unaffected by many times the normal rate of insecticide application.

[0010] Organophosphorus insecticides, which are active against a broad spectrum of insects, have accounted for a large share of all insecticides used. Although organophosphorus insecticides are still used for insect control on many food crops, most residential uses have been phased out in the United States by implementation of the Food Quality Protection Act of 1996. The acute high dose effects of organophosphorus insecticides from intentional and unintentional overdoses or from high-dose worker exposures are well known and include neurological dysfunction that results from the inhibition of enzyme acetylcholinesterase leading to excess acetylcholine in the central and peripheral nervous systems.

[0011] Therefore, one of the challenges of modem agriculture is to address the social demand for sustainability, quality, and safety in agricultural production and to adapt itself to the increasing world population by improving yields and crop tolerance to a changing environment. To address these issues, high productivity in agriculture is necessary that can meet the rising demand of food supply and thereby novel agrochemical combinations are need of today’s times. Seaweeds are multicellular, macroscopic organisms found in coastal, marine ecosystems and possess high quantity of polysaccharides, polyunsaturated fatty acids (PUFA), enzymes, minerals, bioactive enzymes and other components.

[0012] WO202 1069866 relates to a composition of seaweed algal extracts having a dry matter content between 18-36%.

[0013] WO2021140327 relates to a combination comprising: a) at least one seaweed extract b) at least one superabsorbent polymer and c) at least one pesticide.

[0014] WO2022049498 relates to a composition comprising a pesticide and at least one bio-stimulant in an amount sufficient to stimulate or promote plant growth.

[0015] WO2022018745 relates to a synergistic agrochemical mixture for foliar spray and soil application comprising of bioactive amount of diamide insecticide, one or more plant health additives and an insecticide.

[0016] Single actives and double active combinations used over a long period of time have resulted in rampant insect resistance. With the onset of resistance in certain pests, there is a need in the art for a combination of actives that decrease the chances of resistance and improves the spectrum of disease and pest control while simultaneously improving the crop yield and growth.

[0017] There is a continuous need in the domain to have agrochemical combinations and compositions comprising an algal sea-weed extract which are environmentally safe, effective, stable, stimulate crop growth and overcome issues relating to pest resistance and are easy to use. However, an issue that lies with liquid and concentrated algal extract compositions is their degradation and shorter shelf life due to the presence of large amounts of organic content. Moreover, prior art methods of preparing, characterizing, and using compositions derived from algae, have often been ineffective or limited in their applicability with respect to their stability, concentrations and in affecting plant growth and / or health characteristics. There still remains a dire need for algal extract-based compositions which can be applied in low doses to generate higher yield, growth and stress tolerance in plants. The present inventors have surprisingly developed an effective agrochemical combination of insecticides and sea-weed algal extract which overcome the shortcomings of the prior art and those that are encountered in cultivation. The present inventors have also provided an agrochemical combination / composition comprising a sea-weed algal extract and chemical insecticides such that the application dosage of chemical insecticides is significantly reduced.

[0018] The present disclosure aims to provide compositions and methods that are markedly different from such compositions known in the prior art and provide new, eco-friendly, and effective ways of modulating plant health and / or growth characteristics.

[0019] OBJECTS OF THE INVENTION

[0020] It is an object of the present invention to provide a novel and an effective agrochemical combination / composition that improves crop yield and crop growth.

[0021] It is an object of the present invention to provide an agrochemical combination / composition that improves control of insect infestation in crops.

[0022] It is an object of the present invention to provide an agrochemical combination / composition comprising a sea-weed algal extract and chemical insecticides such that the application dosage of chemical insecticides is significantly reduced.

[0023] It is another object of the present invention to provide an agrochemical combination and a composition that broadens the spectrum of control of insect pests, reduces environmental impact, decreases the chances of development of resistance in insects, results in an improved control on the insect pests and overall crop health as well as improved plant vigor in field condition.

[0024] It is another object of the present invention to provide a method of improving crop growth, quality, yield and health.

[0025] SUMMARY OF THE INVENTION

[0026] According to an aspect of the present invention there is provided an agrochemical combination comprising: (i) one or more insecticides of the group selected from pyrethroid(s) and avermectin(s) or combinations thereof; and

[0027] (ii) a sea-weed algal extract.

[0028] According to another aspect of the present invention there is provided an agrochemical combination comprising:

[0029] A) an insecticidal component comprising:

[0030] (i) cypermethrin or its derivatives or isomers,

[0031] (ii) abamectin or its isomers or salts, and

[0032] B) a sea-weed algal extract of Ascophyllum sp.

[0033] According to another aspect of the present invention there is provided an agrochemical combination comprising:

[0034] A) an insecticidal component comprising:

[0035] (i) cypermethrin or its derivatives or isomers,

[0036] (ii) abamectin or its isomers or salts, and

[0037] B) a sea-weed algal extract of Ascophyllum nodosum.

[0038] According to yet another aspect of the present invention there is provided an agrochemical composition comprising:

[0039] A) an insecticidal component comprising one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof;

[0040] B) a sea-weed algal extract of Ascophyllum sp., and

[0041] C) agrochemically acceptable excipient(s).

[0042] According to yet another aspect of the present invention there is provided an agrochemical composition comprising:

[0043] A) an insecticidal component comprising:

[0044] (i) cypermethrin or its derivatives or isomers, and

[0045] (ii) abamectin or its isomers or salts,

[0046] B) a sea-weed algal extract of Ascophyllum nodosum, and

[0047] C) agrochemically acceptable excipient(s). According to yet another aspect of the present invention there is provided a water dispersible granular (WDG) agrochemical composition comprising:

[0048] A) an insecticidal component comprising:

[0049] (i) cypermethrin or its derivatives or isomers,

[0050] (ii) abamectin or its isomers or salts,

[0051] B) a sea-weed algal extract of Ascophyllum nodosum, and

[0052] C) agrochemically acceptable excipient(s).

[0053] According to yet another aspect of the present invention there is provided a method for controlling insect infestation in plants and improving crop quality and yield comprising applying to the plant, plant part, and / or plant propagation material thereof, an effective amount of an agrochemical combination or composition comprising one or more insecticides selected from pyrethroid insecticides and avermectin insecticides or combinations thereof; and a sea-weed algal extract.

[0054] According to yet another aspect of the present invention there is provided a method for controlling insect infestation in plants comprising applying to the plant, plant part, plant organ, and / or plant propagation material thereof, an effective amount of an agrochemical combination or composition comprising cypermethrin, abamectin and a sea-weed algal extract of Ascophyllum nodosum.

[0055] According to yet another aspect of the present invention there is provided a method for improving crop quality and yield comprising applying to the plant, plant part, and / or plant propagation material thereof, an effective amount of a combination or a composition comprising cypermethrin, abamectin and an extract of nodosum.

[0056] According to yet another aspect of the present invention there is provided use of a combination or a composition comprising cypermethrin, abamectin and an extract of A. nodosum for controlling insect infestation in crops, improving crop quality and yield.

[0057] Advantageously, the present invention provides an agrochemical combination / composition comprising a sea-weed algal extract and chemical insecticides such that the application dosage of chemical insecticides is significantly reduced. DETAILED DESCRIPTION OF THE INVENTION

[0058] For the purposes of the following detailed description, it is to be understood that the invention may assume various alternative variations and step sequences, except where expressly specified to the contrary.

[0059] Thus, before describing the present invention in detail, it is to be understood that this invention is not limited to particularly exemplified systems or process parameters that may of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control.

[0060] It should be noted that, as used in this specification, the singular forms “a,” “an” and “the” include plural referents unless the content clearly dictates otherwise. The terms “preferred” and “preferably” refer to embodiments of the invention that may afford certain benefits, under certain circumstances.

[0061] As used herein, the terms “comprising” “including,” “having,” “containing,” “involving,” and the like are to be understood to be open-ended, i.e., to mean including but not limited to. It should be emphasized that the term “comprises / comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components or groups thereof.

[0062] Accordingly, it should be apparent to those skilled in the art that the following description of exemplary embodiments of the present invention are provided for illustration purpose only and not for the purpose of limiting the scope of the invention as defined by the appended claims and their equivalents. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, suitable methods and materials are described herein.

[0063] “About” or “approximately” as used herein is inclusive of the stated value and means within an acceptable range of deviation for the particular value as determined by one of ordinary skill in the art, considering the measurement in question and the error associated with measurement of the particular quantity (i.e., the limitations of the measurement system). For example, “about” can mean within one or more standard deviations, or within ± 10% or ± 5% of the stated value.

[0064] Recitation of ranges of values are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. The endpoints of all ranges are included within the range and independently combinable. As used herein, all numerical values or numerical ranges include integers within such ranges and fractions of the values or the integers within ranges unless the context clearly indicates otherwise. Thus, for example, reference to a range of 90-100%, includes 91%, 92%, 93%, 94%, 95%, 95%, 97%, etc., as well as 91.1%, 91.2%, 91.3%, 91.4%, 91.5%, etc., 92.1%, 92.2%, 92.3%, 92.4%, 92.5%, etc., and so forth. All methods described herein can be performed in a suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.

[0065] The use of the terms “a”, “an” and “the” and similar referents (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms first, second etc., as used herein are not meant to denote any particular ordering, but simply for convenience to denote a plurality of, for example, layers.

[0066] The expression of various quantities in terms of “%” or “% w / w” means the percentage by weight of the composition unless otherwise specified. The term "plants' as used herein, refers to all physical parts of a plant, including seeds, seedlings, saplings, roots, tubers, stems, stalks, foliage and fruits.

[0067] The term "insects" as used herein, includes all organisms in the class "Insecta."

[0068] The term "Insecticidal" as used herein, refers to the ability of a substance to increase mortality or inhibit, growth rate of insects.

[0069] The term "locus" as used herein, refers to the part to which a combination according to the invention is applied. It includes application to an individual plant, a group of plants such as a plant and / or its surrounding, and the region in which plants may be planted as well applied directly to an insect or insects and / or at the vicinity in which they are located.

[0070] The term “ plant propagation material' or “ propagule" is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil. These young plants may be protected before transplantation by a total or partial treatment by immersion.

[0071] The term “ agriculturally acceptable amount of active" refers to an amount of an active that kills or inhibits the plant disease for which control is desired, in an amount not significantly toxic to the plant being treated.

[0072] To “ control' or “ controlling' insects means to inhibit, through a toxic effect, the ability of insect pests to survive, grow, feed, and / or reproduce, or to limit insect- related damage or loss in crop plants. To “control” insects may or may not mean killing the insects, although it preferably means killing the insects.

[0073] As described herein, the term “increased yield' of an agricultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the compositions described herein.

[0074] As used herein, the term “% disease severity” refers to the percentage decay observed in the crops in terms of the percentage of relevant host tissues covered by lesions or damaged by the disease in crops. Severity results from the number and size of the lesions. The % severity indicates the extent of damage caused by the disease.

[0075] As used herein, the term “% disease control” refers to the % control and prevention of a disease in crops.

[0076] The term “fruit set rate ” refers to the rate at which fertilized flowers develop into fruits.

[0077] The term “abortion rate ” refers to the rate at which the growth of the plant part, i.e. fruit or flower is inhibited and thus retarded.

[0078] The term “kg / L” as used herein denotes the concentration of the composition in “kilograms” present “per litre” of the composition.

[0079] The term “kg / ha” as used herein denotes the concentration of the composition in “kilograms” applied “per hectare” of the crop field.

[0080] Each of the embodiments described hereinafter may apply to one or all the aspects described hereinabove. These embodiments are intended to be read as being preferred features of one or all the aspects described hereinabove. Each of the embodiments described hereinafter applies to each of the aspects described hereinabove individually.

[0081] Development of novel and effective agrochemical combinations / compositions as well as new crop management programmes are ongoing because the environmental and financial demands placed on contemporary crop protection compositions are continuously rising, for example, the spectrum of action, toxicity, selectivity, application rate, formation of residues, favourable preparation ability and for solving problems related to resistance to the composition.

[0082] Surprisingly, inventors of the present invention have found that the agrochemical combinations of the present invention comprising insecticidal agents and a sea-weed algal extract provide nutrition to the plants along with reduced pest resistance and long-life cycle, thereby solving current issues associated with conventional crop protection products. The advantages of the invention include managing insect resistance and increased pesticide residues in field slurry and agricultural produce, enhancing crop protection, targeting higher yields and betterquality agricultural products / grain.

[0083] The present inventors have found that the addition of the sea-weed algal extract of Ascophyllum nodosum to an insecticidal combination of a pyrethroid insecticide and an avermectin class of insecticides results in the enhanced control of insect infestation and improved plant growth parameters compared to the insecticidal combination. The addition of the sea-weed algal extract to the combination / composition resulted in the reduction in application dosage of the chemical insecticides. The present disclosure provides that the algal extract along with the insecticides is stable and functionally effective in affecting yield, plant growth and / or health characteristics.

[0084] The present combination broadens the spectrum of control of insect pests and parasitic nematodes, reduces disease severity, reduces environmental impact of synthetic insecticides, decreases the chances of development of insect resistance, improves plant growth, increases yield and enhances overall plant health.

[0085] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0086] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof; and

[0087] (ii) a sea-weed algal extract.

[0088] In another embodiment of the present invention, there is provided an agrochemical combination comprising:

[0089] (i) at least one insecticide of the group selected from pyrethroids; and

[0090] (ii) a sea-weed algal extract.

[0091] In another embodiment of the present invention, there is provided an agrochemical combination comprising:

[0092] (i) at least one insecticide of the group selected from avermectin; and

[0093] (ii) a sea-weed algal extract.

[0094] In a preferred embodiment of the present invention, there is provided an agrochemical combination comprising:

[0095] A) an insecticidal component comprising: (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0096] (ii) at least one insecticide of the group selected from an avermectin insecticide or combinations thereof; and

[0097] B) a sea-weed algal extract.

[0098] In an embodiment, the insecticide is of the group selected from pyrethroids and avermectin or combinations thereof.

[0099] Pyrethroid insecticides may be selected from but are not limited to cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin, zeta- cypermethrin, acrinathrin, allethrin, bioallethrin, esdepallethrine, , bifenthrin, kappa- bifenthrin, bromethrin, butethrin, chlorempenthrin, cyclethrin, cycloprothrin, cyfluthrin, beta- cyfluthrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cyphenothrin, deltamethrin, dimefluthrin, dimethrin, empenthrin, d-fanshiluquebingjuzhi, chloroprallethrin, fenfluthrin, fenpirithrin, fenpropathrin, fenvalerate, esfenvalerate, flucythrinate, fluvalinate, tau-fluvalinate, furamethrin, furethrin, heptafluthrin, imiprothrin, japothrins, kadethrin, methothrin, metofluthrin, epsilon-metofluthrin, momfluorothrin, epsilon-momfluorothrin, pentmethrin, permethrin, biopermethrin, transpermethrin, phenothrin, prallethrin, profluthrin, proparthrin, pyresmethrin, renofluthrin, meperfluthrin, resmethrin, bioresmethrin, cismethrin, tefluthrin, kappa- tefluthrin, tetramethrin, tralomethrin, transfluthrin, etofenprox, halfenprox, silafluofen, Empenthrin [(EZ)- (1R)- isomers], natural pyrethrins such as cinerin-1, cinerin-11, jasmolin-1, jasmolin-11, pyrethrin-1 and pyrethrin-11 or isomers or derivatives or combinations thereof.

[0100] In a preferred embodiment, the pyrethroid insecticide comprises allethrin, bifenthrin, cyhalothrin, cypermethrin, deltamethrin, permethrin, cyfluthrin derivatives or isomers thereof.

[0101] In a more preferred embodiment, the pyrethroid insecticide comprises cypermethrin or its derivatives or isomers. In a preferred embodiment, the pyrethroid insecticide comprises cypermethrin and its isomers selected from alpha-cypermethrin, beta-cypermethrin, theta- cypermethrin and zeta-cypermethrin.

[0102] Avermectin insecticides may be selected from but are not limited to abamectin, emamectin benzoate, lepimectin or combinations thereof.

[0103] In a preferred embodiment, the avermectin insecticide is abamectin.

[0104] In an embodiment, the seaweed extract is from algae obtained from species selected from Ascophyllum sp., Ascophyllum nodosum, Fucus vesiculosus, Laminaria digitata, Laminaria hyperborea, Laminaria saccharina, Eklonia maxima, sargassum spp. Or combinations thereof.

[0105] In a preferred embodiment, the sea-weed algal extract is from Ascophyllum sp.

[0106] In a preferred embodiment, the sea-weed algal extract is from A. nodosum.

[0107] In an embodiment, the concentrated algal extract has a dry matter content of more than 5%, preferably about 5% to about 25%, about 6% to about 25%, about 8% to about 25%, about 10% to about 25%, about 12% to about 25%, about 14% to about 25%, about 16% to about 25%, about 18% to about 25%, more preferably about 20% to about 25%, wherein the dry matter is w / w of the total weight of the extract.

[0108] In an embodiment, the dry matter content of the concentrated algal extract in the composition is more than about 5%, preferably about 5% to about 25%, about 6% to about 25%, about 8% to about 25%, about 10% to about 25%, about 12% to about 25%, about 14% to about 25%, about 16% to about 25%, about 18% to about 25%, more preferably about 20% to about 25%, wherein the dry matter is w / w of the total weight of the composition.

[0109] In an embodiment, the concentrated extract of Ascophyllum nodosum is characterized by the presence of metabolites comprising mannitol, fucoidans, polyphenols, glycine, lamine and such other metabolites.

[0110] The dry matter in the extract or the composition may be analysed by any conventional methods or any other methods known to a person skilled in the art. Such methods include analysis by moisture analyzers (thermobalance) such as, but not limited to, Sartorius MA35 Series Moisture Analyzer.

[0111] The term “dry matter” includes any one or both of inorganic components and organic components. Dry matter is usually measured using available technologies such as thermobalance or other conventional methods known to a person skilled in the art.

[0112] In an embodiment, the concentrated algal extract has a total organic content of more than 5%. In an embodiment, the concentrated algal extract has a total organic content of about 5% to about 100%, about 5% to about 90%, about 5% to about 80%, about 5% to about 70%, about 5% to about 60%, about 5% to about 50%, about 5% to about 40%, about 5% to about 30% and more preferably about 5% to about 20%, wherein the total organic content is w / w content of the total weight of the extract. In another preferred embodiment, the concentrated algal extract has a total organic content of about 11% to about 18%.

[0113] The total organic content in the extract or the composition may be analysed by any conventional methods, or any other methods known to a person skilled in the art. Such methods include analysis by elemental analyzers such as, but not limited to, Thermoscientific, FlashSmart model. The total organic content is calculated as a measure of total carbon content. The formula used for calculation is as follows:

[0114] Total organic content = Total carbon content x 2

[0115] In an embodiment, the application of the seaweed extract of the present invention along with the aforementioned group of insecticides to the plants confers one or more of the following advantages: improved plant nutrient use efficiency, increased flowering, increased fruit set quality, facilitates root growth etc., which are not to be considered as limiting.

[0116] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0117] A) an insecticidal component comprising:

[0118] (i) cypermethrin or its derivatives or isomers,

[0119] (ii) abamectin or its isomer or salts, and B) sea-weed algal extract of Ascophyllum sp.

[0120] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0121] A) an insecticidal component comprising:

[0122] (i) cypermethrin or its derivatives or isomers,

[0123] (ii) abamectin or its isomer or salts, and

[0124] B) sea-weed algal extract of Ascophyllum nodosum.

[0125] In an embodiment of the present invention, there is provided an agrochemical combination comprising: (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof; and (ii) a sea-weed algal extract, wherein the ratio of the insecticidal component to the sea-weed algal extract is ranging from 1 : 100 to 100: 1, 1 :90 to 90: 1, 1 :80 to 80: 1, 1 :70 to 70: 1, 1:60 to 60: 1, 1 :50 to 50: 1, 1 :40 to 40: 1, 1 :30 to 30: 1, 1 :40 to 40: 1, 1 :30 to 30: 1, 1 :20 to 20: 1 and 1 : 10 to 10: 1.

[0126] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0127] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0128] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :30 to 30: 1.

[0129] In a preferred embodiment of the present invention, there is provided an agrochemical combination comprising:

[0130] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0131] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :20 to 20: 1.

[0132] In another preferred embodiment of the present invention, there is provided an agrochemical combination comprising: (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0133] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 : 10 to 10: 1.

[0134] In another preferred embodiment of the present invention, there is provided an agrochemical combination comprising:

[0135] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0136] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :5 to 5: 1.

[0137] In a more preferred embodiment of the present invention, there is provided an agrochemical combination comprising:

[0138] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0139] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is 1 : 10.

[0140] In a more preferred embodiment of the present invention, there is provided an agrochemical combination comprising:

[0141] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof, and

[0142] (ii) a sea-weed algal extract, wherein the ratio of the insecticides to the sea-weed algal extract is 1 :4.

[0143] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0144] A) an insecticidal component comprising;

[0145] (i) cypermethrin or its derivatives or isomers,

[0146] (ii) abamectin or its isomers or salts, and

[0147] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :30 to 30: 1.

[0148] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0149] A) an insecticidal component comprising;

[0150] (i) cypermethrin or its derivatives or isomers,

[0151] (ii) abamectin or its isomers or salts, and

[0152] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :20 to 20: 1.

[0153] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0154] A) an insecticidal component comprising;

[0155] (i) cypermethrin or its derivatives or isomers,

[0156] (ii) abamectin or its isomers or salts, and

[0157] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 : 10 to 10: 1.

[0158] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0159] A) an insecticidal component comprising;

[0160] (i) cypermethrin or its derivatives or isomers,

[0161] (ii) abamectin or its isomers or salts, and

[0162] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is ranging from 1 :5 to 5: 1.

[0163] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0164] A) an insecticidal component comprising;

[0165] (i) cypermethrin or its derivatives or isomers,

[0166] (ii) abamectin or its isomers or salts, and

[0167] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is 1 : 10.

[0168] In an embodiment of the present invention, there is provided an agrochemical combination comprising:

[0169] A) an insecticidal component comprising;

[0170] (i) cypermethrin or its derivatives or isomers,

[0171] (ii) abamectin or its isomers or salts, and

[0172] B) a sea-weed algal extract of Ascophyllum nodosum, wherein the ratio of the insecticides to the sea-weed algal extract is 1 :4.

[0173] In an embodiment of the present invention, the pyrethroid insecticide and avermectin insecticide is in a ratio ranging from 1 : 100 to 100: 1, 1 :90 to 90: 1, 1 :80 to 80: 1, 1 :70 to 70: 1, 1 :60 to 60: 1, 1 :50 to 50: 1, 1:40 to 40: 1, 1 :30 to 30: 1, 1 :40 to 40: 1, 1 :30 to 30: 1, 1 :20 to 20: 1, l : 10 to 10: 1. I :5 to 5: l, l :3 to 3: l, 1 :2.5 to 2.5 : 1 and 1 : 1.

[0174] In an embodiment of the present invention, the pyrethroid insecticide and avermectin insecticide is in a ratio ranging from 1 :3 to 3 : 1.

[0175] In an embodiment of the present invention, cypermethrin and abamectin is in a ratio ranging from 1 :3 to 3 : 1.

[0176] The present invention provides that the actives of the combination can be applied as a tank mix or applied simultaneously or sequentially in no order of preference. The present invention provides that the actives of the combination can be applied with one or more adjuvants.

[0177] The combination of the present invention maybe applied simultaneously as a tank mix or a premix formulation or may be applied sequentially. The application may be made to the soil before emergence of the plants, either pre-planting or post-planting. The application may be made to the soil after emergence of the plants. The application may be made as a foliar spray at different timings during crop development, with either one or more applications early or late post-emergence. The combinations according to the invention can also be incorporated into the soil before, during or after sowing seeds of a crop. These combinations as described above may be applied to the locus of the weeds, in an insecticidally effective amount. The amount of the combination according to the invention to be applied, will depend on various factors, such as the subject of the treatment, for example plants, soil or seeds; the type of treatment, such as, for example spraying, dusting or seed dressing; the purpose of the treatment, such as, for example prophylactic or therapeutic disease control; in case of disease control the type of insect to be controlled or the application time. This amount of the combination of the present invention to be applied can be readily deduced by a skilled agronomist.

[0178] In an embodiment, the agrochemical combinations of the present disclosure are applied as tank mixes or ready mix or as a pre-mix.

[0179] The combinations of the present disclosure may be developed as a pre-mix composition or a kit of parts such that individual actives may be mixed before applying.

[0180] The present invention also provides a composition comprising a combination according to any one of the above-described embodiments, and agrochemically acceptable excipient(s).

[0181] In an embodiment of the present invention, there is provided an agrochemical composition comprising:

[0182] (i) one or more insecticides of the group selected from pyrethroid and avermectin or combinations thereof,

[0183] (ii) a sea-weed algal extract, and

[0184] (iii) agrochemically acceptable excipient(s).

[0185] In a preferred embodiment of the present invention, there is provided an agrochemical composition comprising:

[0186] A) an insecticidal component comprising;

[0187] (i) a pyrethroid insecticide(s) or combinations thereof,

[0188] (ii) an avermectin insecticide or combinations thereof,

[0189] B) a sea-weed algal extract, and

[0190] C) agrochemically acceptable excipient(s).

[0191] In an embodiment, the insecticide is of the group selected from pyrethroid and avermectin or combinations thereof. Pyrethroid insecticides may be selected from but are not limited to cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin, zeta- cypermethrin, acrinathrin, allethrin, bioallethrin, esdepallethrine, , bifenthrin, kappa- bifenthrin, bromethrin, butethrin, chlorempenthrin, cyclethrin, cycloprothrin, cyfluthrin, beta- cyfluthrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cyphenothrin, deltamethrin, dimefluthrin, dimethrin, empenthrin, d-fanshiluquebingjuzhi, chloroprallethrin, fenfluthrin, fenpirithrin, fenpropathrin, fenvalerate, esfenvalerate, flucythrinate, fluvalinate, tau-fluvalinate, furamethrin, furethrin, heptafluthrin, imiprothrin, japothrins, kadethrin, methothrin, metofluthrin, epsilon-metofluthrin, momfluorothrin, epsilon-momfluorothrin, pentmethrin, permethrin, biopermethrin, transpermethrin, phenothrin, prallethrin, profluthrin, proparthrin, pyresmethrin, renofluthrin, meperfluthrin, resmethrin, bioresmethrin, cismethrin, tefluthrin, kappa- tefluthrin, tetramethrin, tralomethrin, transfluthrin, etofenprox, halfenprox, silafluofen, Empenthrin [(EZ)- (1R)- isomers], natural pyrethrins such as cinerin-1, cinerin-11, jasmolin-1, jasmolin-11, pyrethrin-1 and pyrethrin-11 or isomers or derivatives or combinations thereof. In a preferred embodiment, the pyrethroid insecticide comprises allethrin, bifenthrin, cyhalothrin, cypermethrin, deltamethrin, permethrin, cyfluthrin derivatives or isomers thereof.

[0192] In a more preferred embodiment, the pyrethroid insecticide comprises cypermethrin or its derivatives or isomers.

[0193] In a preferred embodiment, the pyrethroid insecticide comprises cypermethrin and its isomers selected from alpha-cypermethrin, beta-cypermethrin, theta- cypermethrin and zeta-cypermethrin.

[0194] Avermectin insecticides may be selected from but are not limited to abamectin, emamectin benzoate, lepimectin or combinations thereof.

[0195] In a preferred embodiment, the avermectin insecticide is abamectin.

[0196] In an embodiment, the seaweed extract is from algae obtained from species selected from Ascophyllum sp., Ascophyllum nodosum, Fucus vesiculosus, Laminaria digitata, Laminaria hyperborea, Laminaria saccharina, Eklonia maxima, sargassum spp. Or combinations thereof. In a preferred embodiment, the sea-weed algal extract is from Ascophyllum sp.

[0197] In a preferred embodiment, the sea-weed algal extract is from Ascophyllum nodosum.

[0198] In a preferred embodiment of the present invention, there is provided an agrochemical composition comprising:

[0199] A) an insecticidal component comprising;

[0200] (i) cypermethrin or its derivatives or isomers,

[0201] (ii) abamectin or its isomers or salts,

[0202] B) a sea-weed algal extract, and

[0203] C) agrochemically acceptable excipient(s).

[0204] In a preferred embodiment of the present invention, there is provided an agrochemical composition comprising:

[0205] A) an insecticidal component comprising;

[0206] (i) cypermethrin or its derivatives or isomers,

[0207] (ii) abamectin or its isomers or salts,

[0208] B) a sea-weed algal extract of Ascophyllum sp. , and

[0209] C) agrochemically acceptable excipient(s).

[0210] In a preferred embodiment of the present invention, there is provided an agrochemical composition comprising:

[0211] A) an insecticidal component comprising;

[0212] (i) cypermethrin or its derivatives or isomers,

[0213] (ii) abamectin or its isomers or salts,

[0214] B) a sea-weed algal extract of Ascophyllum nodosum, and

[0215] C) agrochemically acceptable excipient(s).

[0216] In an embodiment, the total concentration of the insecticide in the composition may be in the range of 0.1 to 99% by weight of the composition.

[0217] In an embodiment, pyrethroid insecticide may be present in an amount ranging from 0.1 to 99% by weight of the composition.

[0218] In an embodiment, avermectin insecticide may be present in an amount ranging from 0.1 to 99% by weight of the composition. In an embodiment, the concentration of the insecticidal component is ranging from 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, 0.1% to 10%, 0.1% to 8%, 0.1% to 6%, 0.1% to 5.5%, 0.1% to 5%, and 0.1% to 3% by weight of the composition.

[0219] In an embodiment, the pyrethroid insecticide of the insecticide component is ranging from 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, and 0.1% to 10% by weight of the composition.

[0220] In an embodiment, the avermectin insecticide is ranging from 0.1% to 50%, 0.1% to 40%, 0.1% to 30%, 0.1% to 20%, and 0.1% to 10% and 0.1% to 5.5% by weight of the composition.

[0221] In a preferred embodiment, the concentration of the pyrethroid is ranging from 0.1% to 10% by weight of the composition.

[0222] In another preferred embodiment, the concentration of the pyrethroid is ranging from 0.1% to 5% by weight of the composition.

[0223] In an embodiment, the concentration of the avermectin insecticide in the composition is ranging from 0.1% to 10% by weight of the composition.

[0224] In another preferred embodiment, the concentration of the avermectin insecticide is ranging from 0.1% to 5% by weight of the composition.

[0225] In an embodiment, the concentration of seaweed extract is ranging from 0.1 to 99%, 1 to 50%, 1 to 40 %, 1 to 35 % and 5% to 35%, by weight of the composition.

[0226] In an embodiment, the concentration of Ascophyllum sp. is ranging from 0.1 to 99%, 1 to 50%, 1 to 40 %, 1 to 35 % and 5% to 35%, by weight of the composition.

[0227] In an embodiment, the concentration of Ascophyllum nodosum is ranging from 0.1 to 99%, 1 to 50%, 1 to 40 %, 1 to 35 % and 5% to 35%, by weight of the composition.

[0228] In an embodiment, the agrochemically acceptable excipient(s) is present in a concentration ranging from 0.1 % to 60%, preferably in a concentration ranging from 0.1% to 50% by weight of the composition. In an embodiment of the present invention, there is provided an agrochemical composition comprising:

[0229] A) an insecticidal component comprising:

[0230] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof in a concentration ranging from 0.1% to 10% w / w,

[0231] (ii) at least one insecticide of the group selected from an avermectin insecticide or combinations thereof in a concentration ranging from 0.1% to 10% w / w;

[0232] B) a sea-weed algal extract in a concentration ranging from 1% to 50% w / w, and

[0233] C) agrochemically acceptable excipient(s).

[0234] In an embodiment of the present invention, there is provided an agrochemical composition comprising:

[0235] A) an insecticidal component comprising;

[0236] (i) cypermethrin or its derivatives or isomers in a concentration ranging from 0.1% to 8% w / w,

[0237] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 5% l i,

[0238] B) a sea-weed algal extract in a concentration ranging from 1% to 40% w / w, and

[0239] C) agrochemically acceptable excipient(s).

[0240] In an embodiment of the present invention, there is provided an agrochemical composition comprising:

[0241] A) an insecticidal component comprising;

[0242] (i) cypermethrin or its derivatives or isomers in a concentration ranging from

[0243] 0.1% to 6% w / w,

[0244] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 3% l i,

[0245] B) a sea-weed algal extract in a concentration ranging from 1% to 35% w / w, and

[0246] C) agrochemically acceptable excipient(s).

[0247] In an embodiment of the present invention, there is provided an agrochemical composition comprising:

[0248] A) an insecticidal component comprising; (i) cypermethrin or its derivatives or isomers in a concentration ranging from

[0249] 0.1% to 5.5% w / w,

[0250] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 2% l i,

[0251] B) a sea-weed algal extract in a concentration ranging from 5% to 35% w / w, and

[0252] C) agrochemically acceptable excipient(s).

[0253] In an embodiment, the constituents of the compositions of the present invention can be tank mixed and sprayed at the locus of the infection or may be alternatively mixed with surfactants and then sprayed.

[0254] In an embodiment, the constituents of the composition of the present invention may be used for foliar application, ground or applications to plant propagation materials, preferably seed applications.

[0255] In an embodiment, the combinations and compositions according to the invention can be converted into customary formulations, typically by mixing the actives in the composition with an inert carrier and adding surfactants and other adjuvants and carriers as needed.

[0256] The combinations can be formulated into solid, or liquid formulations, including but not limited to wettable powders, granules, dusts, Soluble (liquid) concentrates, suspension concentrates, oil in water emulsion, water in oil emulsion, emulsifiable concentrates, capsule suspensions, ZC formulations, oil dispersions or other known formulation types. The composition may also be used for treatment of a plant propagation material such as seeds etc. These formulations are produced in the known manner, for example by mixing the active compound (s) with suitable extenders, that is, liquid solvents and / or solid carriers, optionally with the use of surfactants, that is, emulsifiers and / or dispersants.

[0257] The compositions of the invention may thus be formulated as: a water-soluble concentrate (SL), an emulsifiable concentrate (EC), an emulsion (EW), a microemulsion (ME), a suspension concentrate (SC), an oil-based suspension concentrate (OD), a flowable suspension (FS), a water-dispersible granule (WG), a water-soluble granule (SG), a water-dispersible powder (WP), a water soluble powder (SP), a granule (GR), an encapsulated granule (CG), a fine granule (FG), a macrogranule (GG), an aqueous suspo-emulsion (SE), a micro-encapsulated suspension (CS), and a microgranule (MG).

[0258] In an embodiment, the agrochemical composition is selected from powder for dry soil application (DS), granule / soil applied (GR), Controlled (slow or fast) release granules (CR), jambo bolls or bags (bags in water soluble pouch), water soluble granule (SG), water dispersible granule (WG or WDG), or wettable granules or soil applied granules (SAG).

[0259] The compositions of the present invention can be applied in a variety of ways known to those skilled in the art, at various concentrations. The compositions are useful in preventing, controlling and / or treating insect, nematode and fungal infestations in plants, plant parts and / or surroundings by preemergence or postemergence application to plants, plant parts and / or surroundings is desired. The synergistic compositions of this invention preferably also comprise an agriculturally acceptable carrier therefor. In practice, the composition is the industry for facilitating dispersion. The choice of formulation and mode of application for any given compound may affect its activity, and selection will be made accordingly.

[0260] Using such formulations, either straight (that is undiluted) or diluted with a suitable solvent, especially water, plants, plant parts and / or the surroundings can be treated and protected against insect, nematode and fungi by spraying, pouring immersing or treating. Generally, formulation can be diluted with water having the rate of about 100 mL -5L of composition in 100 L of water. In some embodiments, the composition is formulated as suspension concentrate. For SC formulation, the composition can be diluted with water having the rate of 2L of composition in 100 L of water. For FS formulation, the composition can be diluted with water having a concentration of 360 mL of composition in 100 L of water.

[0261] The composition can be applied with the methods known in the art. These methods include coating, spraying, dipping, soaking, injection, irrigation etc. Further, other biocidally active ingredients or compositions may be combined with the synergistic composition of this invention. As one skilled in the art is aware, in insect and nematode testing, a significant number of factors that are not readily controllable can affect results of individual tests and render them non-reproducible. For example, the results may vary depending on environmental factors, such as amount of sunlight and water, soil type, pH of the soil, temperature, and humidity, among other factors. Also, the depth of planting, the application rate of individual and combined insecticides and sea-weed extract, and the ratio of each insecticide and sea-weed extract, as well as nature of crops being tested, can affect test results. Results may vary from crop to crop within the crop varieties.

[0262] The process for preparing the present novel synergistic composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variation and modification is still covered by the scope of present invention.

[0263] These and other aspects of the invention may become more apparent from the examples set forth herein below. These examples are provided merely as illustrations of the invention and are not intended to be construed as a limitation thereof.

[0264] In a preferred embodiment, the composition of present invention is in the form of wettable granules (WG) or water dispersible granules (WDG’s).

[0265] In an embodiment of the present invention, there is provided a water dispersible granular composition comprising:

[0266] A) an insecticidal component comprising;

[0267] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0268] (ii) at least one insecticide of the group selected from avermectin or combinations thereof;

[0269] B) a sea-weed algal extract, and

[0270] C) agrochemically acceptable excipient(s).

[0271] In a preferred embodiment of the present invention, there is provided a water dispersible granular composition comprising:

[0272] A) an insecticidal component comprising;

[0273] (i) cypermethrin or its derivatives or isomers, (ii) abamectin or its isomers or salts,

[0274] B) a sea-weed algal extract, and

[0275] C) agrochemically acceptable excipient(s).

[0276] In a preferred embodiment of the present invention, there is a water dispersible granular composition comprising:

[0277] A) an insecticidal component comprising;

[0278] (i) cypermethrin or its derivatives or isomers,

[0279] (ii) abamectin or its isomers or salts,

[0280] B) a sea-weed algal extract of Ascophyllum sp. , and

[0281] C) agrochemically acceptable excipient(s).

[0282] In a preferred embodiment of the present invention, there is provided a water dispersible granular composition comprising:

[0283] A) an insecticidal component comprising;

[0284] (i) cypermethrin or its derivatives or isomers,

[0285] (ii) abamectin or its isomers or salts,

[0286] B) a sea-weed algal extract of Ascophyllum nodosum, and

[0287] C) agrochemically acceptable excipient(s).

[0288] In an embodiment of the present invention, there is provided a water dispersible granular composition comprising:

[0289] A) an insecticidal component comprising:

[0290] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof in a concentration ranging from 0.1% to 10% w / w,

[0291] (ii) at least one insecticide of the group selected from an avermectin insecticide or combinations thereof in a concentration ranging from 0.1% to 10% w / w;

[0292] B) a sea-weed algal extract in a concentration ranging from 1% to 50% w / w, and

[0293] C) agrochemically acceptable excipient(s).

[0294] In a preferred embodiment of the present invention, there is provided a water dispersible granule composition comprising:

[0295] A) an insecticidal component comprising; (i) cypermethrin or its derivatives or isomers in a concentration ranging from 0.1% to 8% w / w,

[0296] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 5% l i,

[0297] B) a sea-weed algal extract in a concentration ranging from 1% to 40% w / w, and

[0298] C) agrochemically acceptable excipient(s).

[0299] In a preferred embodiment of the present invention, there is a water dispersible granule composition comprising:

[0300] A) an insecticidal component comprising;

[0301] (i) cypermethrin or its derivatives or isomers in a concentration ranging from

[0302] 0.1% to 6% w / w,

[0303] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 3% l i,

[0304] B) a sea-weed algal extract in a concentration ranging from 1% to 40% w / w, and

[0305] C) agrochemically acceptable excipient(s).

[0306] In a preferred embodiment of the present invention, there is provided a water dispersible granule composition comprising:

[0307] A) an insecticidal component comprising;

[0308] (i) cypermethrin or its derivatives or isomers in a concentration ranging from

[0309] 0.1% to 5.5% w / w,

[0310] (ii) abamectin or its isomers or salts in a concentration ranging from 0.1% to 2% l i,

[0311] B) a sea-weed algal extract in a concentration ranging from 5% to 35% w / w, and

[0312] C) agrochemically acceptable excipient(s).

[0313] In an embodiment, the agrochemically acceptable excipient is in a concentration ranging from 1% to 75% w / w of the composition.

[0314] According to an embodiment of the present disclosure, agrochemically acceptable excipients are selected from one or more of emulsifiers, colorants, thickeners / binders, antifreeze agents, antifoaming agents, antioxidants, solvents, preservatives, glidants, anticaking agents, pH-regulating agents, buffering agents, formulation aids, disintegrants, inert, wetting agents, solid carriers or combinations thereof.

[0315] In another embodiment, emulsifiers which can be advantageously employed herein can be readily determined by those skilled in the art and include various non-ionic, anionic, cationic, and amphoteric emulsifiers, or a blend of two or more emulsifiers. Examples of non-ionic emulsifiers useful in preparing the emulsifiable concentrates include the polyalkylene glycol ethers and condensation products of alkyl and aryl phenols, aliphatic alcohols, aliphatic amines or fatty acids with ethylene oxide, propylene oxides such as the ethoxylated alkyl phenols and carboxylic esters solubilized with the polyol or polyoxyalkylene. Cationic emulsifiers include quaternary ammonium compounds and fatty amine salts. Anionic emulsifiers include the oilsoluble salts (e.g., calcium) of alkylaryl sulfonic acids, oil-soluble salts or sulfated polyglycol ethers and appropriate salts of phosphated polyglycol ether.

[0316] In an embodiment, colorants may be selected from iron oxide, titanium oxide and Prussian Blue, and organic dyestuffs, such as alizarin dyestuffs, azo dyestuffs or metal phthalocyanine dyestuffs, and trace elements, such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.

[0317] In an embodiment, thickener / binders or gelling agent may be selected from but not limited to molasses, granulated sugar, alginates, karaya gum, jaguar gum, tragacanth gum, polysaccharide gum, mucilage, xanthan gum, or combinations thereof. In another embodiment, the binder may be selected from silicates such as magnesium aluminium silicate, polyvinyl acetates, polyvinyl acetate copolymers, polyvinyl alcohols, polyvinyl alcohol copolymers, celluloses, including ethylcelluloses and methylcelluloses, hydroxymethyl celluloses, hydroxypropylcelluloses, hydroxymethylpropyl-celluloses, polyvinylpyrolidones, dextrins, malto-dextrins, polysaccharides, fats, oils, proteins, gum arabics, shellacs, vinylidene chloride, vinylidene chloride copolymers, acrylic copolymers, starches, polyvinylacrylates, zeins, gelatin, carboxymethylcellulose, chitosan, polyethylene oxide, acrylimide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylimide monomers, alginate, ethylcellulose, polychloroprene and syrups or mixtures thereof; polymers and copolymers of vinyl acetate, methyl cellulose, vinylidene chloride, acrylic, cellulose, polyvinylpyrrolidone and polysaccharide; polymers and copolymers of vinylidene chloride and vinyl acetate-ethylene copolymers; combinations of polyvinyl alcohol and sucrose; plasticizers such as glycerol, propylene glycol, polyglycols.

[0318] In another embodiment, antifreeze agent(s) added to the composition may be alcohols selected from the group comprising of but not limited to ethylene glycol, 1,2- propylene glycol, 1,3 -propylene glycol, 1,2-butanediol, 1,3 -butanediol, 1,4-butanediol, 1,4-pentanediol, 3-methyl-l,5-pentanediol, 2,3-dimethyl-2,3-butanediol, trimethylol propane, mannitol, sorbitol, glycerol, pentaerythritol, 1,4-cyclohexanedimethanol, xylenol, bisphenols such as bisphenol A or the like. In addition, ether alcohols such as diethylene glycol, triethylene glycol, tetraethylene glycol, polyoxyethylene or polyoxypropylene glycols of molecular weight up to about 4000, diethylene glycol monomethylether, diethylene glycol monoethylether, triethylene glycol monomethylether, butoxyethanol, butylene glycol monobutylether, dipentaerythritol, tripentaerythritol, tetrapentaerythritol, diglycerol, triglycerol, tetraglycerol, pentaglycerol, hexaglycerol, heptaglycerol, octaglycerol.

[0319] According to an embodiment, antifoam agent may be selected from polydimethoxysiloxane, polydimethyl siloxane, alkyl poly acrylates, castor oil, fatty acids, fatty acids esters, fatty acids sulfate, fatty alcohol, fatty alcohol esters, fatty alcohol sulfate, foot olive oil, mono & di glyceride, paraffin oil, paraffin wax, poly propylene glycol, silicone, silicone oil, vegetable fats, vegetable fats sulfate, vegetable oil, vegetable oil sulfate, vegetable wax, vegetable wax sulfate, agents based on silicon or magnesium stearate, and blend of maltodextrin, and methylated silica.

[0320] The agrochemical composition may also comprise one or more antioxidants. Preferably, the agrochemical formulation comprises an antioxidant. Antioxidants are, for example, amino acids (e.g., glycine, histidine, tyrosine, tryptophan) and derivatives thereof, imidazole and imidazole derivatives (e.g., urocanic acid), peptides, such as, for example, D,L-carnosine, D-carnosine, L-camosine and derivatives thereof (e.g., anserine), carotenoids, carotenes (e.g., a-carotene, P-carotene, lycopene) and derivatives thereof, lipoic acid and derivatives thereof (e.g., dihydrolipoic acid), aurothioglucose, propylthiouracil and further thio compounds (e.g., thioglycerol, thiosorbitol, thioglycolic acid, thioredoxin, glutathione, cysteine, cystine, cystamine and the glycosyl, N-acetyl, methyl, ethyl, propyl, amyl, butyl, lauryl, palmitoyl, oleyl, y-linoleyl, cholesteryl and glyceryl esters thereof), and salts thereof, dilauryl thiodipropionate, distearyl thiodipropionate, thiodipropionic acid and derivatives thereof (esters, ethers, peptides, lipids, nucleotides, nucleosides and salts), and sulfoximine compounds (e.g., buthionine sulfoximines, homocysteine sulfoximine, buthionine sulfones, penta-, hexa-, heptathionine sulfoximine) in very low tolerated doses (e.g., pmol / kg to pmol / kg), also metal chelating agents (e.g., a-hydroxy fatty acids, EDTA, EGTA, phytic acid, lactoferrin), a-hydroxy acids (e.g., citric acid, lactic acid, malic acid), humic acids, bile acid, bile extracts, gallic esters (e.g., propyl, octyl and dodecyl gallate), flavonoids, catechins, bilirubin, biliverdin and derivatives thereof, unsaturated fatty acids and derivatives thereof (e.g., y-linolenic acid, linoleic acid, arachidonic acid, oleic acid), folic acid and derivatives thereof, hydroquinone and derivatives thereof (e.g., arbutin), ubiquinone and ubiquinol, and derivatives thereof, vitamin C and derivatives thereof (e.g., ascorbyl palmitate, stearate, dipalmitate, acetate, Mg ascorbyl phosphates, sodium and magnesium ascorbate, disodium ascorbyl phosphate and sulfate, potassium ascorbyl tocopheryl phosphate, chitosan ascorbate), isoascorbic acid and derivatives thereof, tocopherols and derivatives thereof (e.g., tocopheryl acetate, linoleate, oleate and succinate, tocophereth-5, tocophereth-10, tocophereth-12, tocophereth-18, tocophereth-50, tocophersolan), vitamin A and derivatives (e.g., vitamin A palmitate), the coniferyl benzoate of benzoin resin, rutin, rutinic acid and derivatives thereof, disodium rutinyl disulfate, cinnamic acid and derivatives thereof (e.g., ferulic acid, ethyl ferulate, caffeic acid), kojic acid, chitosan glycolate and salicylate, butylhydroxytoluene, butylhydroxyanisol, nordihydroguaiacic acid, nordihydroguaiaretic acid, trihydroxybutyrophenone, uric acid and derivatives thereof, mannose and derivatives thereof, selenium and selenium derivatives (e.g., selenomethionine), stilbenes and stilbene derivatives (e.g., stilbene oxide, trans-stilbene oxide). According to the disclosure, suitable derivatives (salts, esters, sugars, nucleotides, nucleosides, peptides, and lipids) and mixtures of these specified active ingredients or plant extracts (e.g., teatree oil, rosemary extract and rosemarinic acid) which comprise these antioxidants can be used. In general, mixtures of the aforementioned antioxidants are possible. According to an embodiment, examples of suitable solvents are water, oils of vegetable, or derivatives. In principle, solvent mixtures may also be used.

[0321] In another embodiment, suitable preservatives are for example benzothiazoles, l,2-benzisothiazolin-3-one, sodium dichloro-s-triazinetrione, sodium benzoate, potassium sorbate, l,2-phenyl-isothiazolin-3-one, inter chloroxylenol paraoxybenzoate butyl, benzoic acid, or combinations thereof.

[0322] In another embodiment, suitable inert are for example precipitated silica, titanium dioxide, kaolin, bentonite, soapstone, talc, attapulgite, ceramic, montmorillonite, pumice, sepiolite, diatomaceous earth, sand, clay, dolomite, calcite, magnesium oxides, magnesium carbonates, sucrose, com starch, citric acid and its salts, or combinations thereof.

[0323] In an embodiment, other formulation aids may be selected from fillers such as calcium sulphate dihydrate (gypsum).

[0324] In an embodiment, the wetting agent may be selected from dialkyl naphthalene sulphonate formaldehyde condensate, dialkyl naphthalene sulphonate sodium salt and sodium naphthalene sulfonate - formaldehyde condensate.

[0325] According to an embodiment of the present disclosure, the agrochemical composition comprises from about 1% w / w to about 30% w / w of the agrochemically acceptable excipient of total weight of the composition.

[0326] Examples of the solid carriers used in formulation include but are not limited to fine powders or granules such as minerals such as kaolin clay, attapulgite clay, bentonite, montmorillonite, acid white clay, pyrophyllite, talc, diatomaceous earth and calcite; natural organic materials such as com rachis powder and walnut husk powder; synthetic organic materials such as urea; salts such as calcium carbonate and ammonium sulfate; synthetic inorganic materials such as synthetic hydrated silicon oxide; and as a liquid carrier, aromatic hydrocarbons such as xylene, alkylbenzene and methylnaphthalene; alcohols such as 2-propanol, ethyleneglycol, propylene glycol, and ethylene glycol monoethyl ether; ketones such as acetone, cyclohexanone and isophorone; vegetable oil such as soybean oil and cotton seed oil; petroleum aliphatic hydrocarbons, esters, dimethylsulfoxide, acetonitrile and water. Examples of the surfactants include but are not limited to anionic surfactants such as alkyl sulfate ester salts, alkylaryl sulfonate salts, dialkyl sulfosuccinate salts, polyoxyethylene alkylaryl ether phosphate ester salts, ligno sulfonate salts and naphthalene sulfonate formaldehyde polycondensates; and nonionic surfactants such as polyoxyethylene alkyl aryl ethers, polyoxyethylene alkylpolyoxypropylene block copolymers and sorbitan fatty acid esters and cationic surfactants such as alkyltrimethylammonium salts.

[0327] The formulations of the present invention may be applied either straight (that is undiluted) or diluted with a suitable solvent, especially water, to the plants, plant parts and / or the surrounding can be treated and protected against insects by spraying, pouring or immersing.

[0328] In an embodiment of the present invention, the combination is provided at a dosage in the range of about 0.1 L / ha to about 20 L / ha. In a preferred embodiment, the dosage of the combination is about 0.1 to about 10 L / ha. In another preferred embodiment, the dosage of the combination is about 0.1 to about 5 L / ha. In a preferred embodiment, the dosage of the combination is about 0.5 L / ha.

[0329] In an embodiment of the present invention, the composition is provided at a dosage in the range of about 0.1 kg / ha to about 20 kg / ha. In a preferred embodiment, the dosage of the combination is about 0.1 to about 10 kg / ha. In another preferred embodiment, the dosage of the combination is about 0.1 to about 5 kg / ha. In a preferred embodiment, the dosage of the combination is about 0.5 kg / ha.

[0330] The doses herein indicated are given as illustrative examples of the method according to the invention. A person skilled in the art will know how to adapt the application doses, notably according to the nature of the plant or crop to be treated.

[0331] The composition of the present invention can be applied with the active insecticide component and sea-weed algal extract obtained from a separate formulation source and mixed together for example as a tank-mix, ready-to-apply, spray broth, or slurry, optionally with other pesticides, or the active insecticide components can be obtained as a single formulation mixture for example as a pre-mix, concentrate, formulated compound (or product), and mixed together with the sea-weed algal extract before being applied. The active compound content in the commercially available formulations can vary.

[0332] The combinations of the present invention may be sold as a pre-mix composition or a kit of parts such that individual actives may be mixed before spraying. Alternatively, the kit of parts may contain the pre-mixed pyrethroid insecticide and / or the pre-mixed avermectin insecticide and the sea-weed algal extract packaged separately, such that the two / three components may be tank mixed before spraying.

[0333] In an embodiment, the present invention provides a kit of parts comprising at least one insecticide, a sea-weed algal extract optionally with one or more agrochemically acceptable excipients and an instruction information to apply said components at the locus of the plant to be treated against pests specifically insects.

[0334] The composition of the present invention maybe applied simultaneously as a tank mix or a formulation or may be applied sequentially. The application may be made to the soil before emergence of the plants, either pre -planting or post-planting. The application may be made as a foliar spray at different timings during crop development, with either one or two or three applications early or late post-emergence.

[0335] The active components of the composition can be applied to the plants, plant parts and / or their surroundings where control is desired either simultaneously or sequentially, for example on the same day in any order. The composition may be applied just once or a plurality of times. Preferably, composition is applied a plurality of times, for example 1 to 6 times preferably about 2 to 5 times within an interval of 5 days to 20 days for example the compositions are applied within an interval of 10 days to 15 days after each application.

[0336] Depending on the nature of the active substance employed, the formulations according to the invention are suitable for any useful plants, for example grain crops, root crops, oil crops, vegetables, spices, ornamentals and the like, for example for the treatment of following plants: durum wheat and other wheat species, oats, rye, barley, maize, including fodder maize and sugar maize, soybeans, bras sicas, okra, cotton, sunflower, bananas, rice, oilseed rape, beet, sugarbeet, fodder beet, aubergines, potatoes, turf, grass seed, tomatoes, leek, pumpkin / squash, cabbage, lettuces, bell peppers, cucumbers, melons, beans, peas, garlic, onions, carrots, tobacco, grapes, petunias, geraniums, pelargoniums, pansies, and the like.

[0337] In a preferred embodiment, the compositions of present invention improve crop yield, crop growth and control insect infestation in cotton plant. More, specifically, the claimed composition improves number of cotton bolls at harvest.

[0338] The combinations of the present invention may be provided as a pre-mix composition or a kit of parts such that individual actives may be mixed before spraying.

[0339] One or more of the components may already be combined together or preformulated. In those embodiments where more than two components are provided in a kit, the components may already be combined and as such are packaged in a single container such as a vial, bottle, can, pouch, bag and / or canister. In other embodiments, two or more components of a kit may be packaged separate from one another, i.e., they are not pre-formulated. As such, a kit may include one or more separate containers such as vials, cans, bottles, pouches, bags and / or canisters, each container containing a separate component of the insecticidal combination.

[0340] In both forms, an individual component of the kit may be applied separate from or together with the other components of the kit or as a component of a combination or a composition.

[0341] The disclosure also provides a kit comprising insecticidal composition for the controlling the growth of insects and instructions for use. The instructions for use typically comprise instructions for the application of the insecticidal composition to a plant, or a locus, or a plant propagation material thereof.

[0342] In an embodiment, the kit of parts comprises at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof, at least one insecticide of the group selected from avermectin or combinations thereof; and a seaweed algal extract.

[0343] According to an embodiment, the kit of parts comprises:

[0344] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof, (ii) at least one insecticide of the group selected from avermectin or combinations thereof;

[0345] (iii) a sea-weed algal extract, and

[0346] (iv)an instruction manual for use.

[0347] According to an embodiment, the kit of parts comprises:

[0348] A) an insecticidal component comprising;

[0349] (i) cypermethrin or its derivatives or isomers,

[0350] (ii) abamectin or its isomers or salts,

[0351] B) a sea-weed algal extract of Ascophyllum sp., and

[0352] C) an instruction manual for use.

[0353] According to an embodiment, the kit of parts comprises:

[0354] A) an insecticidal component comprising;

[0355] (i) cypermethrin or its derivatives or isomers,

[0356] (ii) abamectin or its isomers or salts,

[0357] B) a sea-weed algal extract of Ascophyllum nodosum, and

[0358] C) agrochemically acceptable excipient(s).

[0359] Accordingly, ingredients may be pre-mixed and optional third active is admixed with an adjuvant such that two components may be tank mixed before spraying.

[0360] The present invention provides a method for controlling pest infestation in plants and improving yield comprising applying to the plant, part of a plant, plant organ, and / or plant propagation material thereof, a synergistically effective amount of combination comprising at least one insecticide of the class selected from pyrethroid and avermectin or combinations thereof; and a sea-weed algal extract.

[0361] In an embodiment of the present invention there is provided a method for reducing overall damage of plants and plant parts in addition to losses in harvested fruits or vegetables caused by insects, nematodes and / or phytopathogens and improving the yield and / or vigour of the plant comprising applying simultaneously or sequentially, a synergistically effective amount of the agrochemical combination comprising at least one insecticide and sea-weed algal extract on the plant, plant parts, harvested fruits, vegetables and / or plant's locus of growth. When it is said that the present method is capable of "improving the yield and / or vigour" of a plant, the present method results in an increase in either the yield, as described above, or the vigor of the plant, as described above, or both the yield and the vigor of the plant.

[0362] In a preferred embodiment, the present invention provides a method for improving the number of cotton bolls at harvest by applying the combination of the present invention.

[0363] The compositions according to the present invention is synergistically effective for the following plant treatments against insects.

[0364] In an embodiment the insect pests controlled by the combinations of the present invention may belong to the class Insecta, Arachnida and Nematoda. Exemplary pests may include: from the order Lepidoptera, pests such as Acleris spp., Adoxophyes spp., Aegeria spp., Agrotis spp., Alabama argillaceae, Amylois spp., Anticarsia gemmatalis, Archips spp., Argyrotaenia spp., Autographa spp., Busseola fusca, Cadra cautella, Carposina nipponensis, Chilo spp., Choristoneura spp., Clysia ambiguella, Cnaphalocrocis spp., Cnephasia spp., Cochylis spp., Coleophora spp., Crocidolomia spp., Cryptophlebia leucotreta, Crysodeixis includens, Cydia spp., Diatraea spp., Dipar op sis castanea, Earias spp., Elasmopalpus spp., Ephestia spp., Eucosma spp., Eupoecilia ambiguella, Euproctis spp., Euxoa spp., Grapholita spp., Hedya nubiferana, Heliothis spp., Hellula undalis, Hyphantria cunea, Keiferia ly coper sicella, Leucoptera scitella, Lithocollethis spp., Lobesia botrana, Lymantria spp., Lyonetia spp., Malacosoma spp., Mamestra brassicae, Manduca sexta, Operophtera spp., Ostrinia nubilalis, Pammene spp., Pandemis spp., Panolis flammea, Pectinophora gossypiella, Phthorimaea operculella, Pieris rapae, Pieris spp., Plutella xylo Stella, Prays spp., Scirpophaga spp., Sesamia spp., Sparganothis spp., Spodoptera spp., Synanthedon spp., Thaumetopoea spp., Tortrix spp., Trichoplusia ni and Yponomeuta spp.; from the order Coleoptera, pest such as Agriotes spp., Anthonomus spp., Atomaria linearis, Ceutorhynchus spp., Chaetocnema tibialis, Cosmopolites spp., Curculio spp., Dermestes spp., Diabrotica spp., Epilachna spp., Eremnus spp., Gonocephalum spp., Heteronychus spp., Leptinotarsa decemlineata, Lissorhoptms spp., Melolontha spp., Orycaephilus spp., Otiorhynchus spp., Phlyctinus spp., Phyllotreta spp., Popillia spp., Protostrophus spp., Psylliodes spp., Rhizopertha spp., Scarabeidae, Sitophilus spp., Sitotroga spp., Tenebrio spp., Tribolium spp. and Trogoderma spp.; from the order Orthoptera, pests such as Blatta spp., Blattella spp., Gryllotalpa spp., Leucophaea maderae, Locusta spp., Periplaneta spp. and Schistocerca spp.; from the order Isoptera, pests such as Reticulitermes spp.; from the order Psocoptera pest such as, Liposcelis spp.; from the order Anoplura, pests such as Haematopinus spp., Linognathus spp., Pediculus spp., Pemphigus spp. and Phylloxera spp.; from the order Mallophaga pests such as Damalinea spp. and Trichodectes spp.; rom the order Thysanoptera, pests such as Frankliniella spp., Hercinothrips spp., Taeniothrips spp., Thrips palmi, Thrips tabaci and Scirtothrips aurantii; from the order Heteroptera, pests such as Dichelops melacanthus, Distantiella theobroma, Dysdercus spp., Euchistus spp., Eurygaster spp., Leptocorisa spp., Nezara spp., Piesma spp., Rhodnius spp., Sahlbergella singular is, Scotinophara spp. and Triatoma spp.; from the order Homoptera, insect pests such as Aleurothrixus floccosus, Aleyrodes brassicae, Aonidiella spp., Aphididae, Aphis spp., Aspidiotus spp., Bemisia tabaci, Ceroplaster spp., Chrysomphalus aonidium, Chrysomphalus dictyospermi, Coccus hesperidum, Empoasca spp., Eriosoma larigerum, Erythroneura spp., Gascardia spp., Laodelphax spp., Lecanium comi, Lepidosaphes spp., Macrosiphus spp., Myzus spp., Nephotettix spp., Nilaparvata spp., Paratoria spp., Pemphigus spp., Pianococcus spp., Pseudaulacaspis spp., Pseudococcus spp., Psylla spp., Pulvinaria aethiopica, Quadraspidiotus spp., Rhopalosiphum spp., Saissetia spp., Scaphoideus spp., Schizaphis spp., Sitobion spp., Trialeurodes vaporariorum, Trioza erytreae and Unaspis citri; from the order Hymenoptera, insect pests such as Acromyrmex, Athalia rosae, Atta spp., C ephus spp., Diprion spp., Diprionidae, Gilpinia polytoma, Hoplocampa spp., Lasius spp., Monomorium pharaonis, Neodiprion spp., Solenopsis spp. and Vespa spp.; from the order Diptera, insect pests such as Antherigona soccata, Bibio hortulanus, Ceratitis spp., Chrysomyia spp., Culex spp., Cuterebra spp., Dacus spp., Delia spp., Drosophila melanogaster, Liriomyza spp., Melanagromyza spp., Orseolia spp., Oscinella frit, Pegomyia hyoscyami, Phorbia spp., Rhagoletis pomonella, Sciara spp.; from the order Acarina, pests such as Acarus siro, Aceria sheldoni, Aculus schlechtendali, Amblyomma spp., Argas spp., Brevipalpus spp., Bryobia praetiosa, Calipitrimerus spp., Chorioptes spp., Dermanyssus gallinae, Eotetranychus carpini, Eriophyes spp., Hyalomma spp., Olygonychus pratensis, Ornithodoros spp., Panonychus spp., Phyllocoptruta spp. (such as Phyllocoptruta oleivora), Polyphagotarsonemus latus, Psoroptes spp., Rhipicephalus spp., Rhizoglyphus spp., Sarcoptes spp., Tarsonemus spp. and Tetranychus spp.; and from the class Nematoda, the species of Meloidogyne spp. (for example, Meloidogyne incoginita and Meloidogyne javanica), Heterodera spp. (for example, Heterodera glycines, Heterodera schachtii, Heterodora avenae and Heterodora trifolii), Globodera spp. (for example, Globodera rostochiensis), Radopholus spp. (for example, Radopholus similes), Rotylenchulus spp., Pratylenchus spp. (for example, Pratylenchus neglectans and Pratylenchus penetrans), Aphelenchoides spp., Helicotylenchus spp., Hoplolaimus spp., Paratrichodorus spp., Longidorus spp., Nacobbus spp., Subanguina spp. Belonlaimus spp., Criconemella spp., Criconemoides spp. Ditylenchus spp., Dolichodorus spp., Hemicriconemoides spp., Hemicycliophora spp., Hirschmaniella spp., Hypsoperine spp., Macroposthonia spp., Melinius spp., Punctodera spp., Quinisulcius spp., Scutellonema spp., Xiphinema spp., and Tylenchorhynchus spp .

[0365] In a preferred embodiment, the target insects controlled by the combinations of the present invention are Haritalodes sp, Aphis sp, Jacobiella sp, Pectinophora sp.

[0366] In a preferred embodiment, the target insects controlled by the combinations of the present invention are Haritalodes derogate, Aphis gossypii, Jacobiella facialis, Pectinophora gossypiella.

[0367] In a preferred embodiment, the target larvae controlled by the combinations of the present invention are Earias insulana, Thaumatotibia leucotreta.

[0368] In an embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination comprising:

[0369] (i) at least one insecticide of the group selected from pyrethroids; and

[0370] (ii) a sea-weed algal extract.

[0371] In an embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination comprising: (i) at least one insecticide of the group selected from avermectins; and

[0372] (ii) a sea-weed algal extract.

[0373] In a preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination comprising:

[0374] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0375] (ii) at least one insecticide of the group selected from avermectin or combinations thereof; and

[0376] (iii) a sea-weed algal extract.

[0377] In another preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination comprising:

[0378] (i) cypermethrin or its derivatives or isomers,

[0379] (ii) abamectin or its isomer or salts, and

[0380] (iii) a sea-weed algal extract of Ascophyllum sp..

[0381] In another preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination comprising:

[0382] (i) cypermethrin or its derivatives or isomers,

[0383] (ii) abamectin or its isomer or salts, and

[0384] (iii) a sea-weed algal extract of Ascophyllum nodosum.

[0385] In an embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a cotton plant, plant part or plant propagation material thereof an agrochemical combination comprising:

[0386] (i) at least one insecticide of the group selected from pyrethroids; and

[0387] (ii) a sea-weed algal extract. In an embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a cotton plant, plant part or plant propagation material thereof an agrochemical combination comprising:

[0388] (i) at least one insecticide of the group selected from avermectins; and

[0389] (ii) a sea-weed algal extract.

[0390] In a preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a cotton plant, plant part or plant propagation material thereof an agrochemical combination comprising:

[0391] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0392] (ii) at least one insecticide of the group selected from avermectin or combinations thereof; and

[0393] (iii) a sea-weed algal extract.

[0394] In another preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a cotton plant, plant part or plant propagation material thereof an agrochemical combination comprising:

[0395] (i) cypermethrin or its derivatives or isomers,

[0396] (ii) abamectin or its isomer or salts, and

[0397] (iii) a sea-weed algal extract of Ascophyllum sp..

[0398] In another preferred embodiment, there is provided a method of controlling insect infestation and improving crop quality, growth and yield comprising applying at a locus of a cotton plant, plant part or plant propagation material thereof an agrochemical combination comprising:

[0399] (i) cypermethrin or its derivatives or isomers

[0400] (ii) abamectin or its isomer or salts, and

[0401] (iii) a sea-weed algal extract of Ascophyllum nodosum. In an embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0402] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0403] (ii) at least one insecticide of the group selected from avermectin or combinations thereof; and

[0404] (iii) a sea-weed algal extract, wherein the application is made at a rate ranging from 0.1 to 20 kg / ha.

[0405] In another embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0406] (i) cypermethrin or its derivatives or isomers,

[0407] (ii) abamectin or its isomer or salts, and

[0408] (iii) a sea-weed algal extract of Ascophyllum sp., wherein the application is made at a rate ranging from 0.1 to 20 kg / ha.

[0409] In another embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0410] (i) cypermethrin or its derivatives or isomers,

[0411] (ii) abamectin or its isomer or salts, and

[0412] (iii) a sea-weed algal extract of Ascophyllum sp., wherein the application is made at a rate ranging from 0.1 to kg / ha.

[0413] In an embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0414] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0415] (ii) at least one insecticide of the group selected from avermectin or combinations thereof; and

[0416] (iii) a sea-weed algal extract, wherein the application is made at a rate ranging from 0.1 to 10 kg / ha.

[0417] In another embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0418] (i) cypermethrin or its derivatives or isomers,

[0419] (ii) abamectin or its isomer or salts, and

[0420] (iii) a sea-weed algal extract of Ascophyllum sp., wherein the application is made at a rate ranging from 0.1 to 10 kg / ha.

[0421] In another embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0422] (i) cypermethrin or its derivatives or isomers,

[0423] (ii) abamectin or its isomer or salts, and

[0424] (iii) a sea-weed algal extract of Ascophyllum nodosum, wherein the application is made at a rate ranging from 0.1 to 10 kg / ha.

[0425] In a preferred embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0426] (i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,

[0427] (ii) at least one insecticide of the group selected from avermectin or combinations thereof; and

[0428] (iii) a sea-weed algal extract, wherein the composition is applied at a rate ranging from 0.1 to 5 kg / ha.

[0429] In another preferred embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0430] (i) cypermethrin or its derivatives or isomers,

[0431] (ii) abamectin or its isomer or salts, and

[0432] (iii) a sea-weed algal extract of Ascophyllum sp., wherein the composition is applied at a rate ranging from 0.1 to 5 kg / ha. In another preferred embodiment, there is provided a method of controlling insect infestation comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical combination / composition comprising:

[0433] (i) cypermethrin or its derivatives or isomers,

[0434] (ii) abamectin or its isomer or salts, and

[0435] (iii) a sea-weed algal extract of Ascophyllum nodosum, wherein the composition is applied at a rate ranging from 0.1 to 5 kg / ha.

[0436] In accordance with the aforesaid embodiment, the present invention provides a method of improving crop quality, growth and yield comprising by applying the composition in rates as mentioned above.

[0437] The present agrochemical combinations are environmentally safe, effective, stimulate crop growth and overcome issues relating to pest resistance.

[0438] The present invention provides the use of the present agrochemical combination for the following:

[0439] 1. controlling insect infestation in crops,

[0440] 2. improving crop quality and yield,

[0441] 3. Reduction in % damage in fruits, eg reduction in % damage of cotton bolls,

[0442] 4. Increase in yield of crop or fruit,

[0443] 5. Increase in fruit set rate and reduction in abortion rate.

[0444] In view of the above, it will be seen that the several advantages of the disclosure are achieved, and other advantageous results are attained. Although the present disclosure has been disclosed in full, it will be understood that numerous additional modifications and variations could be made thereto without departing from the scope of the disclosure. The embodiments may be combined together for better understanding of the disclosure, without departing from the scope of the disclosure.

[0445] In another embodiment, alternative or multiple embodiments of the disclosure disclosed herein are not to be construed as limitations. Each embodiment can be referred to and claimed individually or in any combination with other embodiments of the disclosure. One or more embodiments of the disclosure can be combined together to exhibit the teaching of the invention, without departing from the scope of the disclosure.

[0446] The invention shall now be described with reference to the following specific examples. It should be noted that the examples appended below illustrate rather than limit the invention and that those skilled in the art will be able to design many alternative embodiments without departing from the scope of the present invention.

[0447] EXAMPLES:

[0448] The following examples are meant to illustrate the present invention. The examples are presented to exemplify the invention and are not to be considered as limiting the scope of the invention.

[0449] EXAMPLE - 1:

[0450] Water dispersible granules formulation:

[0451] Cypermethrin 36 g / Kg + Abamectin 14 g / Kg + Ascophyllum nodosum extract 194 g / Kg Table 1 : Formulation recipe q.s. : quantity sufficient

[0452] Process

[0453] The ingredients ammonium sulphate, kaolin, precipitated silica, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, dialkyl napthalene sulfonate sodium salt and abamectin were blend and mixed well to obtain a homogenous mixture of the components. The said mixture was milled and passed through wet sieve to obtain a uniform abamectin premix which was further milled with particle size distribution (d90) which is 4 - 20 microns.

[0454] In another suitable vessel, A. nodosum extract along with the aqueous emulsion with polysiloxane and emulsifier, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, cypermethrin were added with continuous mixing and stirred for 10 to 60 min to obtain a uniform distribution of cypermethrin and obtain a cypermethrin-premix / emulsion.

[0455] Abamectin premix was charged in a dough maker and the dough was prepared by charging Cypermethrin premix / emulsion. Prepared dough was processed through granulator to obtain the granules which were then dried in fluidized bed dryer. The dried granules were sieved through a suitable mesh sized sieve.

[0456] EXAMPLE -2:

[0457] Trials for testing efficacy of the combination (Cypermethrin + Abamectin + Extract of Ascophyllum nodosum on cotton plant

[0458] Example 2(a): Efficacy of present agrochemical combination for reducing % plant infested and attacked by insects

[0459] Trials were conducted to test the efficacy of the combination of cypermethrin, abamectin and the extract of Ascophyllum nodosum on cotton plants for controlling infestation by insects of Haritalodes derogate and Jacobiella facialis at an application amount of 0.5 L / ha.

[0460] Treatment no. 2 is an emulsifiable concentrate formulation of cypermethrin and abamectin (100 EC). Treatment no. 3 refers to the emulsifiable concentrate formulation of cypermethrin and abamectin which is tank mixed with the extract of Ascophyllum nodosum. Table 2(a): % plants attacked by adult insects

[0461] Observations: A considerable reduction in the number of cotton plants attacked by the

[0462] J. facialis and H. derogate was observed in cotton crop treated with the combination of cypermethrin, abamectin and the extract of A. nodosum compared to the insecticidal combination of cypermethrin and abamectin.

[0463] Example 2(b): Efficacy of present agrochemical combination for reducing insect larvae

[0464] Trials were conducted to test the efficacy of the combination of cypermethrin, abamectin and the extract of Ascophyllum nodosum on cotton plants for controlling infestation by larvae of Earias insulana, Thaumatotibia leucotreta at an application amount of 0.5 L / ha. Treatment no. 1(b) refers to the emulsifiable concentrate formulation of cypermethrin and abamectin which is tank mixed with the extract of Ascophyllum nodosum. Table 2(b): Reduction in number of larvae

[0465] Observations: A considerable reduction in the number of cotton plants attacked by the J. facialis and H. derogate was observed in cotton crop treated with the combination of cypermethrin, abamectin and the extract of A. nodosum compared to the insecticidal combination of cypermethrin and abamectin and the untreated crop.

[0466] Example 2(c): Efficacy of present agrochemical combination for improving plant characters

[0467] Trials were conducted to test the efficacy of the combination of cypermethrin, abamectin and the extract of Ascophyllum nodosum on cotton plants for improving plant characters at an application amount of 0.5 L / ha.

[0468] Table2(c): Improvement in cotton plant characteristics

[0469] Observations: A considerable increase in the yield of cotton was observed in cotton crop treated with the combination of cypermethrin, abamectin and the extract of Ascophyllum nodosum in treatments 1 and 2. Further, in soils that are nutrient deficient, chlorosis or yellow discoloration of cotton may be a result of this deficiency. The reduction in the % of yellow cotton was observed for crops treated with the agrochemical combination compared to the insecticidal combination of cypermethrin and abamectin and the untreated crop.

[0470] Conclusion: From the above data, it was concluded that the agrochemical combination of cypermethrin, abamectin and the extract of Ascophyllum nodosum of the present invention shows an improved control over the insects and larvae infestation in cotton plant, improves yield of cotton and reduces the yellowing of cotton. The said agrochemical combination was observed to have enhanced insecticidal control compared to the insecticidal combination of cypermethrin and abamectin.

[0471] EXAMPLE -3:

[0472] Water dispersible granules / Wettable Granules (WG) formulation:

[0473] Cypermethrin 18 g / Kg + Abamectin 7 g / Kg + extract Ascophyllum nodosum 97 g / Kg

[0474] WG

[0475] Table 3: Formulation recipe

[0476] Process

[0477] The ingredients ammonium sulphate, kaolin, precipitated silica, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, dialkyl napthalene sulfonate sodium salt and abamectin were blend and mixed well to obtain a homogenous mixture of the components. The said mixture was milled and passed through wet sieve to obtain a uniform abamectin premix which was further milled with particle size distribution (d90) which is 4 - 20 microns.

[0478] In another suitable vessel, A. nodosum extract along with the aqueous emulsion with polysiloxane and emulsifier, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, cypermethrin were added with continuous mixing and stirred for 10 to 60 min to obtain a uniform distribution of cypermethrin and obtain a cypermethrin-premix / emulsion.

[0479] Abamectin premix was charged in a dough maker and the dough was prepared by charging Cypermethrin premix / emulsion. Prepared dough was processed through granulator to obtain the granules which were then dried in fluidized bed dryer. The dried granules were sieved through a suitable mesh sized sieve.

[0480] EXAMPLE -4:

[0481] Water dispersible granules / Wettable Granules (WG) formulation:

[0482] Cypermethrin 36 g / Kg + Abamectin 14 g / Kg + extract oi Ascophyllum nodosum 200 g / Kg WG

[0483] Table 4: Formulation recipe Process

[0484] The ingredients ammonium sulphate, kaolin, precipitated silica, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, dialkyl napthalene sulfonate sodium salt and abamectin were blend and mixed well to obtain a homogenous mixture of the components. The said mixture was milled and passed through wet sieve to obtain a uniform abamectin premix which was further milled with particle size distribution (d90) which is 4 - 20 microns.

[0485] In another suitable vessel, A. nodosum extract along with the aqueous emulsion with polysiloxane and emulsifier, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, cypermethrin were added with continuous mixing and stirred for 10 to 60 min to obtain a uniform distribution of cypermethrin and obtain a cypermethrin-premix / emulsion.

[0486] Abamectin premix was charged in a dough maker and the dough was prepared by charging Cypermethrin premix / emulsion. Prepared dough was processed through granulator to obtain the granules which were then dried in fluidized bed dryer. The dried granules were sieved through a suitable mesh sized sieve.

[0487] EXAMPLE -5:

[0488] Water dispersible granules / Wettable Granules (WG) formulation:

[0489] Cypermethrin 54 g / Kg + Abamectin 21 g / Kg + extract oi Ascophyllum nodosum 300 g / Kg WG

[0490] Table 5: Formulation recipe

[0491] Process

[0492] The ingredients ammonium sulphate, kaolin, precipitated silica, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, dialkyl napthalene sulfonate sodium salt and abamectin were blend and mixed well to obtain a homogenous mixture of the components. The said mixture was milled and passed through wet sieve to obtain a uniform abamectin premix which was further milled with particle size distribution (d90) which is 4 - 20 microns.

[0493] In another suitable vessel, A. nodosum extract along with the aqueous emulsion with polysiloxane and emulsifier, sodium lignosulfonate, calcium lignosulfonate, sodium lauryl sulfate, cypermethrin were added with continuous mixing and stirred for 10 to 60 min to obtain a uniform distribution of cypermethrin and obtain a cypermethrin-premix / emulsion.

[0494] Abamectin premix was charged in a dough maker and the dough was prepared by also charging in the cypermethrin premix. Prepared dough was processed through granulator to obtain the granules which were then dried in fluidized bed dryer. The dried granules were sieved through a suitable mesh sized sieve.

[0495] EXAMPLE -6:

[0496] Dry matter content and total organic content / total carbon content of Ascophyllum Nodosum Extract

[0497] Dry matter content of Ascophyllum Nodosum Concentrated Extract was determined using thermobalance (Sartorius MA35 Series Moisture Analyzer).

[0498] Dry matter content of Ascophyllum Nodosum Concentrated Extract and composition of Example 1 was found to be in the range of 20% to 25%.

[0499] An elemental analyser Thermoscientific - FlashSmart model was used to measure carbon content of the Ascophyllum Nodosum Concentrated Extract (Table 1). Organic matter content was calculated by following formula

[0500] Organic matter content = Carbon content (Ascophyllum Nodosum Extract) x 2

[0501] The organic matter content of the Ascophyllum Nodosum Concentrated Extract was found to be in the range of 11% to 18%.

[0502] EXAMPLE -7:

[0503] Efficacy of the agrochemical composition of cypermethrin abamectin and extract of Ascophyllum nodosum on the growth characters in cotton crop

[0504] Trials were conducted to test the efficacy of the composition of cypermethrin, abamectin and the extract of Ascophyllum nodosum on cotton plants for improving plant / crop growth characters. The agrochemical composition in the form of a water dispersible granule formulation of the present invention was used for the purposes of evaluating the efficacy in improving plant growth parameters. The said agrochemical composition in the form of a water dispersible granule (prepared as per Example- 1 above) formulation of the present invention was used in treatments 2 and 3 for the purposes of evaluating the efficacy of the said composition in reducing the number of attacks in cotton crop. Treatment 4 relates to EC composition of cypermethrin and abamectin.

[0505] An inadequate supply of organic nutrients increases ethylene production, conditions that decrease photosynthesis tend to increase boll shedding, therefore it is imperative to determine the fruit set rate and the abortion rate of the cotton bolls.

[0506] Table 6: % Fruit set rate and abortion rate of cotton bolls

[0507] Observations: The fruit-set rate was observed to be 92% and 94% in the cotton crops treated with the agrochemical composition of the present invention (treatments 2 and 3 respectively), while it was observed to be 66.19% in crops treated with the cypermethrin and abamectin (market reference product - treatment 4) and 58.56% on the untreated cotton crop.

[0508] Further, an increased fruit-set rate than the reference product (treatment no. 4) was observed. As for capsular retention, the abortion rate is significantly higher on untreated cotton (31,95%) compared to treated cotton crops. The crops treated with the agrochemcial composition of cypermethrin + abamectin + extract of Ascophyllum nodosum make it possible to maintain an increased boll number compared to the untreated crops which experienced boll shedding.

[0509] Conclusion: It is evident from Table 6, it was concluded that the agrochemical composition of cypermethrin, abamectin and the extract of Ascophyllum nodosum of the present invention shows an improvement in plant growth parameters.

[0510] EXAMPLE -7:

[0511] Efficacy of the agrochemical composition of cypermethrin abamectin and extract of Ascophyllum nodosum on bud attacks and on yield

[0512] Trials were conducted to test the efficacy of the composition of cypermethrin, abamectin and the extract of Ascophyllum nodosum on cotton plants for improving plant growth characters. The said agrochemical composition in the form of a water dispersible granule formulation of the present invention was used in treatments 2 and 3 for the purposes of evaluating the efficacy of the said composition in reducing the number of attacks in cotton crop. Treatment 4 relates to EC composition of cypermethrin and abamectin.

[0513] Table 7 (a): % unripe cotton bolls attacked by insects Table 7 (b): % Mature cotton bolls attacked by insects

[0514] Table 7 (c): % Yield

[0515] Conclusion: From the above data, it was concluded that the agrochemical composition of cypermethrin, abamectin and the extract of Ascophyllum nodosum of the present invention shows an improved control over the insect infestation in cotton plant and improves yield of cotton. More importantly, the said agrochemical combination was observed to have enhanced insecticidal control compared to the insecticidal combination of cypermethrin and abamectin. It is to be understood that the present invention is susceptible to modifications, changes and adaptations by those skilled in the art. Such modifications, changes, adaptations are intended to be within the scope of the present invention.

Claims

We claim,1. An agrochemical combination comprising:A) an insecticidal component comprising,(i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof, and(ii) at least one insecticide of the group selected from avermectin or combinations thereof, andB) a sea-weed algal extract.

2. The combination as claimed in claim 1, said combination comprising a pyrethroid insecticide selected from the group comprising cypermethrin, allethrin, bifenthrin, cyhalothrin, deltamethrin, permethrin, cyfluthrin derivatives or isomers thereof.

3. The combination as claimed in claim 1, said combination comprising an avermectin insecticide selected from the group comprising abamectin, emamectin benzoate, lepimectin or combinations thereof.

4. The combination as claimed in claim 1, wherein the sea-weed algal extract is of Ascophyllum sp.

5. The combination as claimed in claim 1, said combination comprising:A) an insecticidal component comprising,(i) cypermethrin or its derivatives or isomers,(ii) abamectin or its isomers or salts, andB) a sea-weed algal extract of Ascophyllum nodosum.

6. The combination as claimed in claim 5, wherein the sea-weed algal extract comprises dry matter content ranging from 5% w / w to 25% w / w of the total weight of the extract.

7. The combination as claimed in claim 1, wherein the ratio of the insecticidal component to the sea-weed algal extract is ranging from 1 : 100 to 100: 1.

8. An agrochemical composition comprising:A) an insecticidal component comprising,(i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof, and(ii) at least one insecticide of the group selected from avermectin or combinations thereof,B) a sea-weed algal extract of Ascophyllum sp, andC) agrochemically acceptable excipients.

9. The composition as claimed in claim 8, said composition comprising:A) an insecticidal component comprising,(i) cypermethrin or its derivatives or isomers,(ii) abamectin or isomers or salts thereof, and,B) a sea-weed algal extract of Ascophyllum nodosum,' andC) agrochemically acceptable excipients.

10. The composition as claimed in claim 8, said composition is formulated as a water-dispersible granule (WDG), a suspension concentrate (SC) and a flowable suspension (FS).

11. The composition as claimed in claim 10, the water dispersible granule (WDG) composition comprising:A) an insecticidal component comprising,(i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,(ii) at least one insecticide of the group selected from avermectin or combinations thereof,B) a sea-weed algal extract of Ascophyllum sp., andC) agrochemically acceptable excipients.

12. The composition as claimed in claim 11, said composition comprising:A) an insecticidal component comprising:(i) cypermethrin in a concentration ranging from 0.1% to 10% w / w,(ii) abamectin in a concentration ranging from 0.1% to 10% w / w;B) a sea-weed algal extract of Ascophyllum nodosum in a concentration ranging from 1% to 50% w / w, andC) agrochemically acceptable excipient(s).

13. A method of controlling insect infestation and improving crop quality and yield, said method comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical composition comprising a combination of:A) an insecticidal component comprising;(i) at least one insecticide of the group selected from pyrethroid insecticide(s) or combinations thereof,(ii) at least one insecticide of the group selected from avermectin or combinations thereof, andB) a sea-weed algal extract of Ascophyllum sp.

14. The method as claimed in claim 13, the method comprising applying at a locus of a plant, a plant part or a plant propagation material an agrochemical composition comprising a combination of:(i) cypermethrin or its derivatives or isomers,(ii) abamectin or its isomer or salts, and(iii) sea-weed algal extract of Ascophyllum sp.

15. A kit of parts comprising the combination as claimed in claim 5, said kit comprising:(i) cypermethrin or its derivatives or isomers,(ii) abamectin or its isomer or salts, and(iii) sea-weed algal extract of Ascophyllum nodosum.

16. Use of the combination as claimed in claim 1, the use of said combination for controlling insect infestation in crops, improving crop quality and yield.

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