Composition containing hyaluronic acid, including hydrophobic nanoparticles

Abstract

COMPOSITION COMPRISING HYALURONIC ACID, INCLUDING HYDROPHOBIC NANOPARTICLES. The present invention relates to a composition comprising: (a) at least one cationic polymer; (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives; (b-2) at least one second anionic polymer different from the (b-1) first anionic polymer; (c) at least one non-polymeric acid or a salt thereof; (d) at least one optional metal salt; (e) at least one fatty acid; and (f) water, under specific conditions. The composition according to the present invention may be stable, transparent, or translucent. Furthermore, the composition according to the present invention may deliver hyaluronic acids and / or hyaluronic acid derivatives deeper into a keratinous substance such as skin. Figure for the abstract: none

Description

Description Title of the invention: COMPOSITION COMPRISING OF HYALURONIC ACID, INCLUDING NANO HYDROPHOBIC PARTICLES technical field

[0001] = The present invention relates to a composition comprising a nanoparticle including hyaluronic acid or its salts, as well as a cosmetic process using the composition. PREVIOUS ART

[0002] = Hyaluronic acid is a predominant glucosaminoglycan found in the skin. Thus, fibroblasts primarily synthesize collagens, glyco- matrix proteins other than collagens (fibronectin, laminin), protein- glycans and elastin. Keratinocytes, on the other hand, primarily synthesize sulfated glycosaminoglycans and hyaluronic acid. Hyaluronic acid is also called hyaluronan (HA).

[0003] = Hyaluronic acid is present in a free state in the epidermis and in the dermis and is responsible for skin turgor. This polysaccharide can actually retain a a large volume of water, corresponding to 1000 times its weight. In this sense, hyaluronic acid Luronique plays an important role in increasing the amount of bound water in the tissues, as well as in the mechanical properties of the skin and in the formation of wrinkles.

[0004] = Hyaluronic acid is widely used as a cosmetic ingredient because of its high moisturizing effects.

[0005] — Document JP-A-2014-114272 discloses a complex particle including acid hyaluronic acid, chitosan and an anionic polymer other than hyaluronic acid. DISCLOSURE OF THE INVENTION

[0006] It has been found that a complex particle including hyaluronic acid, such as the one disclosed in JP-A-2014-114272, sometimes has difficulty penetrating a keratinous substance such as skin.

[0007] — Furthermore, in certain cases, a composition including such a complex particle must be stable without causing precipitation, and transparent or translucent with a lower turbidity level. Furthermore, it is preferable that the complex particle be small so that it has a nanometric size (less than 1000 nm), in terms of penetration capacity into a keratinous substance such as skin.

[0008] — Thus, an objective of the present invention is to provide a stable composition, transparent or translucent, comprising a nanoparticle including hyaluronic acid luronique, which could deliver hyaluronic acid deeper into a keratinous substance such as the skin, The above objective of the present invention can be achieved by a composition, preferably a cosmetic composition, and more preferably a cosmetic composition for the skin, comprising nanoparticles including: (a) at least one cationic polymer; (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives: (b-2) at least a second anionic polymer that is different from the (b-1) first anionic polymer; (c) at least one non-polymeric acid or a salt thereof; (d) at least one optional metal salt; and (e) at least one fatty acid, And ({) of water, in which The quantity of nanoparticles is 0.01% to 0.58% by weight, preferably 0.05% to 0.57% by weight and more preferably 0.1% to 0.56% by weight, relative to the total weight of the composition. the weight ratio of the quantity of the (a) cationic polymer(s) to the total weight of the nanoparticles is 0.06 to 0.25, the weight ratio of the quantity of the (b-2) second anionic polymer to the total weight of the nanoparticles is 0.26 or more, the weight ratio of the quantity of the non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles is 0.03 or more, the weight ratio of the quantity of the (d) optional metal salt(s) to the total weight of the nanoparticles is 0.30 or less, and the weight ratio of the quantity of the fatty acid(s) to the total weight of the nanoparticles is 0.001 or more. The (a) cationic polymer may have at least one positively chargeable and / or positively charged fraction selected from the group consisting of a primary, secondary or tertiary amino group, a quaternary ammonium group, a guanidine group, a biguanide group, an imidazole group, an imino group and a pyridyl group. The (a) cationic polymer may be selected from the group consisting of alkyldiallylamine cyclopolymers and dialkyldiallylammonium cyclopolymers such as (co)polydiallyldialkyl ammonium chloride, (co)polyamines such as chitosans and (co)polylysines, cationic (co)polyamino acids such as collagen and arginine / lysine polypeptide, cationic cellulose polymers, and their salts. The weight ratio of the quantity of the (a) cationic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention can be from 0.06 to 0.24, preferably from 0.07 to 0.22, and more preferably from 0.08 to 0.20. The first (b-1) anionic polymer may have a molecular weight of 2,000 kDa or less, preferably 1,000 kDa or less, and more preferably 100 kDa or less. The weight ratio of the quantity of the (b-1) first anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention can be from 0.01 to 0.8, preferably from 0.02 to 0.7, and more preferably from 0.03 to 0.6. The (b-2) second anionic polymer may be chosen from the group consisting of carrageenan, pectin and a mixture thereof. The weight ratio of the quantity of the (b-2) second anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention can be from 0.26 to 0.9, preferably from 0.28 to 0.8, and more preferably from 0.30 to 0.7. (c) non-polymer acid or salt(s) thereof may be selected from the group consisting of phytic acid, citric acid, lactic acid and a mixture thereof. The weight ratio of the quantity of the (c) non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles in the composition according to the present invention can be from 0.03 to 0.6, preferably from 0.035 to 0.5, and more preferably from 0.04 to 0.4. The (d) optional metal salt may be chosen from polyvalent metal salts, preferably divalent metal salts, more preferably calcium salts such as calcium chloride and calcium pyrrolidone carboxylate, and magnesium salts such as magnesium chloride. The weight ratio of the quantity of (d) optional metal salt to the total weight of nanoparticles in the composition according to the present invention can be from 0.001 to 0.30, preferably from 0.005 to 0.25, and more preferably from 0.01 to 0.20. The (e) fatty acid can be chosen from saturated and unsaturated fatty acids, linear or branched in C4-C,,, preferably Ce-Cao, more preferably C:-C;s. The weight ratio of the quantity of (e) fatty acid to the total weight of nanoparticles in the composition according to the present invention can be from 0.001 to 0.4, preferably from 0.002 to 0.3, and more preferably from 0.003 to 0.2. The quantity of (f) water in the composition according to the present invention may be from 50% to 95% by weight, preferably from 60% to 90% by weight and more preferably from 70% to 85% by weight, relative to the total weight of the composition. The present invention also relates to a cosmetic process for a keratinous substance such as skin, comprising: the application, on the keratinous substance, of the composition according to the present invention. Best embodiment of the invention After careful research, the inventors discovered that it is possible to offer a stable, transparent or translucent composition, comprising a nanoparticle including hyaluronic acid, which could deliver hyaluronic acid deeper into a keratinous substance such as skin. Thus, the composition according to the present invention, which is preferably a cosmetic composition, and more preferably a cosmetic composition for the skin, comprises nanoparticles including: (a) at least one cationic polymer; (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives: (b-2) at least a second anionic polymer that is different from the (b-1) first anionic polymer; (c) at least one non-polymeric acid or a salt thereof; (d) at least one optional metal salt; and (e) at least one fatty acid, And ({) of water, in which The quantity of nanoparticles is 0.01% to 0.58% by weight, preferably 0.05% to 0.57% by weight and more preferably 0.1% to 0.56% by weight, relative to the total weight of the composition. the weight ratio of the quantity of the (a) cationic polymer(s) to the total weight of the nanoparticles is 0.06 to 0.25, the weight ratio of the quantity of the (b-2) second anionic polymer to the total weight of the nanoparticles is 0.26 or more, the weight ratio of the quantity of the non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles is 0.03 or more, the weight ratio of the quantity of the (d) optional metal salt(s) per the ratio to the total weight of the nanoparticles is 0.30 or less, and the weight ratio of the quantity of the fatty acid(s) to the total weight of the nanoparticles is 0.001 or more. The term "nanoparticle" refers to a particle with a size or diameter less than 1000 nm. The size or diameter of a nanoparticle can be measured using a dynamic light scattering method. This particle diameter can be based on a volume-average diameter. The composition according to the present invention may include nanoparticles comprising (a) cationic polymer(s), (b-1) first anionic polymer(s), (b-2) second anionic polymer(s), (c) non-polymeric acid(s) or salt(s) thereof, (d) optional metal salt(s), and (e) fatty acid(s). (b-1) the first anionic polymer is selected from hyaluronic acids and their derivatives. Thus, the nanoparticles include at least one hyaluronic acid and / or at least one hyaluronic acid derivative. Nanoparticles can be particles of polyionic complexes that are formed by one or more polyionic complexes. The (a) cationic polymer can be made hydrophobic by the (e) fatty acid. The cationic group, such as a quaternary ammonium group in the (a) cationic polymer, can interact ionically with the anionic carboxyl group of the (D) fatty acid. Since the (e) fatty acid has a hydrophobic fraction, the (a) cationic polymer can be made ionically hydrophobic and thus possess sufficient hydrophobicity. Therefore, nanoparticles, including the (a) cationic polymer, can be made hydrophobic by the (e) fatty acid. Surprisingly, hydrophobic nanoparticles can penetrate more deeply into a keratinous substance such as skin, compared to non-hydrophobic nanoparticles. Nanoparticles can exert cosmetic effects based on hyaluronic acid and / or hyaluronic acid derivatives. Furthermore, the composition according to the present invention can provide cosmetic effects, such as moisturizing effects, based on hyaluronic acid and / or hyaluronic acid derivatives. The composition according to the present invention may be stable, transparent, or translucent. Furthermore, the composition according to the present invention may include nanoparticles containing hyaluronic acid, with an increased capacity for penetration into a keratinous substance such as skin. Thus, the composition according to the present invention can deliver hyaluronic acids and / or hyaluronic acid derivatives deeper into a keratinous substance such as skin. The composition, process and other properties according to the present invention will be described in more detail below. [Nanoparticle] The composition according to the present invention comprises nanoparticles including: (a) of at least one cationic polymer, (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives: (b-2) at least a second anionic polymer that is different from the (b-1) first anionic polymer; (c) at least one non-polymeric acid or a salt thereof; (d) at least one optional metal salt; and (e) at least one fatty acid. Ingredients (a) to (e) above, in particular ingredients (a), (b-1), (b-2) and (c) can form a polyionic complex. The polyionic complex can take the form of a nanoparticle. Two or more different types of nanoparticles may be present in combination. Thus, a single type of nanoparticle or a combination of different types of nanoparticles may be present in the composition according to the present invention. The size or diameter of the nanoparticle can be from 5 nm to 500 nm, preferably from 10 nm to 400 nm, more preferably from 20 nm to 300 nm, and even more preferably from 30 nm to 200 nm. The diameter size can be an average size or diameter based on volume. The quantity of nanoparticles in the composition according to the present invention can be from 0.01% to 0.58% by weight, preferably from 0.05% to 0.57% by weight, more preferably from 0.1% to 0.56% by weight, even more preferably from 0.2% to 0.55% by weight, relative to the total weight of the composition. (Cationic polymer) The composition according to the present invention may comprise (a) at least one cationic polymer. Two or more different types of cationic polymers may be used in combination. Thus, a single type of cationic polymer or a combination of different types of cationic polymers may be used. The (a) cationic polymer has a positive charge density. The charge density of the (a) cationic polymer can be between 0.01 meg / g and 20 meq / g, preferably between 0.05 and 15 meq / g and, more preferably, between 0.1 and 10 meg / g. It may be preferable that the molecular weight of the (a) cationic polymer be equal to or greater than 500, preferably equal to or greater than 1,000, more preferably equal to or greater than 2,000, and even more preferably equal to or greater than 3,000. Unless otherwise defined in the descriptions, "molecular weight" means an average molecular weight by weight. The (a) cationic polymer may have at least one positively chargeable fraction and / or positively charged, selected from the group consisting of a primary, secondary, or tertiary amino group, a quaternary ammonium group, a guanidine group, a biguanide group, an imidazole group, an imino group, and a pyridyl group. The term "amino group" (primary) here means a -NH₂ group. The (a) cationic polymer may be a homopolymer or a copolymer. "Copolymer" includes both copolymers obtained from two types of monomers and those obtained from more than two types of monomers, such as terpolymers obtained from three types of monomers. (a) The cationic polymer may be selected from natural and synthetic cationic polymers. Non-limiting examples of (a) cationic polymers are given below. (1) Homopolymers and copolymers derived from acrylic or methacrylic esters and amides and comprising at least one motif selected from the motifs of the following formulas: Ra Ru Rs ii Î pat Grau Seam 3:1 * z ft D È2 NH } io Î &â to A '= $ 3 —N Micmonhe "emo PEN RU MR 3 ET CPR Ke Ra i not + NH | = Ê£& + Ras Re 1 + D in which: Ry and Ra, which may be identical or different, are chosen from hydrogen and alkyl groups comprising 1 to 6 carbon atoms, for example methyl and ethyl groups; R3, which may be the same or different, is chosen from hydrogen and CH; The symbols A, which may be identical or different, are chosen from linear or branched alkyl groups comprising 1 to 6 carbon atoms, for example 2 to 3 carbon atoms, and hydroxyalkyl groups comprising 1 to 4 carbon atoms; R4, Rs and Re, which may be identical or different, are chosen from alkyl groups comprising from 1 to 18 carbon atoms and benzyl groups, and in at least one embodiment, alkyl groups comprising from 1 to 6 carbon atoms and X is an anion derived from an inorganic or organic acid, such as methosulfate anions and halides, for example chloride and bromide. The copolymers of family (1) above may also include at least one motif derived from comonomers which may be selected from acrylamides, methacrylamides, diacetone acrylamides, acrylamides and methacrylamides substituted on the nitrogen atom with (C,-C4) lower alkyl groups, groups derived from acrylic or methacrylic acids and their esters, vinyllactams such as vinylpyrrolidone and vinylcaprolactam and vinyl esters. Here are some examples of copolymers from family (1): quaternized copolymers of acrylamide and dimethylaminoethyl methacrylate with dimethyl sulfate or with a dimethyl halide, copolymers of acrylamide and methacryloyloxyethyltrimethylammonium chloride described, for example, in European Patent Application No. 0 080 976, copolymers of acrylamide and methacryloyloxyethyltrimethylammonium methosulfate, quaternized or non-quaternized methacrylate or vinylpyrrolidone / dialkylaminoalkyl acrylate copolymers, described, for example, in French Patents No. 2,077,143 and 2,393,573, dimethylaminoethyl / vinylca- methacrylate terpolymers prolactam / vinylpyrrolidone, vinylpyrrolidone / methacrylamidopropyldimethylamine copolymers, quaternized vinylpyrrolidone / dimethylaminopropylmethacrylamide copolymers and crosslinked polymers of methacryloyloxy(C,-C4)alkyltri(C,-C4)alkylammonium salts such as polymers obtained by homopolymerization of quaternized dimethylaminoethyl methacrylate with methyl chloride, or by copolymerization of acrylamide with quaternized dimethylaminoethyl methacrylate with methyl chloride, the homo- or copolymerization being followed by crosslinking with a compound containing an olefinic unsaturation, in particular methylenebisa-erylamide. (2) Cationic cellulose polymers such as cellulose ether derivatives comprising one or more quaternary ammonium groups described, for example, in French patent No. 1,492,597, such as the polymers marketed under the names "JR" (JR 400, JR 125, JR 30M) or "LR" (LR 400, LR 30M) by Union Carbide Corporation. These polymers are also defined in the CTFA dictionary as quaternary ammonium compounds of hydroxyethylcellulose that have reacted with an epoxide substituted with a trimethylammonium group. It is preferable that cationic cellulose polymers have at least one quaternary ammonium group, preferably a quaternary trialkylammonium group and, more preferably, a quaternary trimethylammonium group. The quaternary ammonium group may be present in a quaternary ammonium group which can be represented by the following chemical formula (I): (D OH Ra BR, Hs to H —o—FropEHE-d arr, x OH OR in which each of the groups R, and R; designates an alkyl group C,-C3, preferably a methyl or ethyl group and, more preferably, a methyl group, R3 designates an alkyl group C,- C4, preferably a methyl or ethyl group and, more preferably, a methyl group, X- denotes an anion, preferably a halide and, more preferably, a chloride, n denotes an integer between 0 and 30, preferably between 0 and 10 and, more preferably, equal to 0, and R4 designates a C,-C4 alkylene group, preferably an ethylene or propylene group. The leftmost ether bond (-O-) in the above chemical formula (I) can attach to the sugar ring of the polysaccharide. It is preferable that the group containing the quaternary ammonium be -O-CH; - CH(OH)-CH,-N+(CH,);. (3) Cationic cellulose polymers such as cellulosic copolymers and cellulose derivatives grafted with a water-soluble quaternary ammonium monomer and described, for example, in US Patent No. 4,131,576, such as hydroxyalkylcelluloses, for example, hydroxymethyl-, hydroxyethyl- and hydroxypropylcelluloses grafted, with, for example, a salt selected from the salts of methacryloyl-ethyltrimethylammonium, methacrylamidopropyltrimethylammonium and dimethyldiallylammonium. Commercial products made from these polymers include, for example, products marketed under the names “Celquat® L 200” and “Celquat® H 100” by the company National Starch. (4) Non-cellulosic cationic polysaccharides described in US patents Nos. 3,589,578 and 4,031,307, such as guar gums comprising cationic tri-kylammonium groups, cationic hyaluronic acid, and hydroxypropyltrimonium dextran chloride. Guar gums modified with a salt, for example, the chloride, of 2,3-epoxypropyltrimethylammonium (hydroxypropyltrimonium guar chloride) may also be used. These products are marketed, for example, under the trade names JAGUAR® C13 S, JAGUAR® C15, JAGUAR® C17 and JAGUAR® C162 by the company MEYHALL. (5) Polymers comprising piperazinyl units and divalent alkylene or hydroxyalkylene groups comprising straight or branched chains, optionally interrupted with at least one entity selected from oxygen, sulfur, nitrogen, aromatic and heterocyclic rings, as well as the oxidation and / or quaternization products of these polymers. These polymers are described, for example, in French patents Nos. 2,162,025 and 2,280,361. (6) Water-soluble polyaminoamides prepared, for example, by polycondensation of an acidic compound with a polyamine; these polyaminoamides being optionally crosslinked with an entity selected from epihalohydrins; diepoxides; dianhydrides; unsaturated dianhydrides; diunsaturated derivatives; bishalohydrins; bisazetidiniums; bishaloacyidiamines; bisalkyl halides; oligomers resulting from the reaction of a difunctional compound that is reactive with an entity selected from bishalohydrins; bisazetidiniums, bishaloacyldiamines, bisalkyl halides; epihalohydrins; dihydroxides and bisunsaturated derivatives; the crosslinking agent being used in an amount ranging from 0.025 to 0.35 mole per amine group of polyaminoamides; these polyaminoamides being optionally alkylated or, if they include at least one tertiary amine function, they may be quaternized.These polymers are described, for example, in French patents No. 2,252,840 and 2,368,508. (7) Polyamino-amide derivatives resulting from the condensation of polyalkylene polyamines with polycarboxylic acids, followed by alkylation with difunctional agents, for example, adipic acid polymers / dialkylaminohydroxyalkyldialkylenetriamine in which the alkyl group comprises from 1 to 4 carbon atoms, such as methyl, ethyl, and propyl groups, and the alkylene group comprises from 1 to 4 carbon atoms, such as an ethylene group. These polymers are described, for example, in French patent No. 1,583,363. In at least one embodiment, these derivatives may be selected from polymers of adipic acid / dimethylaminohydroxypropyldiethylenetriamine. (8) Polymers obtained by reacting a polyalkylene polyamine comprising two primary amine groups and at least one secondary amine group with a dicarboxylic acid selected from diglycolic acid and saturated dicarboxylic aliphatic acids comprising from 3 to 8 carbon atoms. The molar ratio of the polyalkylene polyamine to the dicarboxylic acid may vary from 0.8:1 to 1.4:1; the resulting polyaminoamide with epichlorohydrin in a molar ratio of epichlorohydrin to the secondary amine group of the polyaminoamide ranging from 0.5:1 to 1.8:1. These polymers are described, for example, in US Patent Nos. 3,227,615 and 2,961,347. (9) Alkyldiallylamine cyclopolymers and dialkyldiallyl-ammonium cyclopolymers, such as homopolymers and copolymers comprising, as the principal chain constituent, at least one motif selected from motifs of formulas (Ia) and (Ib): (there) ÂCHK 2" > ——(CHAt — CRr OR) — 0H — H:CA 0H ke UT = FAX v = Ris Ru (Ib) SGHak 1°, $“ ——(OHaie — CR GR) ——CH2== Ra \ 0078 i END in which: kett, which can be identical or different, are equal to Ô or |, the sum k+t being equal to 1; Ry; is chosen from among the hydrogen and methyl groups; Ryg and Ry1, which may be identical or different, are chosen from alkyl groups comprising 1 to 6 carbon atoms, hydroxyalkyl groups in which the alkyl group comprises, for example, 1 to 5 carbon atoms, and lower amidoalkyl groups (C,-C4), or Ryp and R, which can form, with the atom of nitrogen to which they are attached, heterocyclic groups such as piperidinyl and morpholinyl and Y' is an anion such as bromide, chloride, acetate, borate, citrate, tartrate, bisulfate, bisulfite, sulfate, and phosphate. These polymers are described, for example, in French patent No. 2,080,759 and its certificate of addition 2,190,406. In one embodiment, Ryp and R,,, which may be identical or different, are selected from alkyl groups comprising 1 to 4 carbon atoms. Examples of such polymers include (co)polydiallyldialkyl ammonium chloride, such as the dimethylidiethylammonium chloride homopolymer sold under the name "MERQUAT® 100" by CALGON (and its low weight-average molecular weight counterparts), and the copolymers of diallyl-dimethylammonium chloride and acrylamide sold under the name "MERQUAT® 550". Quaternary dijammonium polymers comprising at least one repeating unit of formula (ID): (ID Ris fs $ : vs pee Apr Me B-—— 4 x ia Riu in which: Ris, Ri4, Ris, and R16, which may be identical or different, are chosen from aliphatic, alicyclic, and aliphatic aryl groups comprising from 1 to 20 carbon atoms and lower hydroxyalkyl aliphatic groups; or Ry3, R14, Rys, and R16, together or separately, with the nitrogen atoms to which they are attached, heterocycles comprising alternatively a second heteroatom other than nitrogen; or Ry3, R14, R1s, and R15, which may be identical or different, are chosen from linear or branched C1-C1 alkyl groups substituted by at least one group chosen from alkyl groups, ester groups, acyl groups, amide groups, CO-OR7-E groups, and CO-NH-R7-E groups, where R7 is an alkylene group and E is a group quaternary ammonium; A, and B, which may be identical or different, are chosen from polymethylene groups comprising from 2 to 20 carbon atoms, which may be linear or branched, saturated or unsaturated, and which may include, bonded or intercalated in the main chain, at least one entity chosen from aromatic rings, oxygen, sulfur, sulfoxide groups, sulfone groups, disulfide groups, amino groups, alkylamino groups, hydroxyl groups, quaternary ammonium groups, ureido groups, amide groups, and ester groups, and X- denotes an anion derived from an inorganic or organic acid; it being understood that A,, Ry3 and R;s can form, with the two nitrogen atoms to which they are attached, a piperazine ring; If A is chosen from linear or branched, saturated or unsaturated alkylene or hydroxyalkylene groups, B can be chosen from: -(CH,),-CO-E-OC-(CH;),- where E' is chosen from: a) glycolic residues of formula -OZO-, where Z is chosen from linear or branched hydrocarbon groups and groups of the following formulas: -(CH,-CH,-O),-CH,-CH-- -[CH,-CH(CH3)-O],-CH,-CH(CH3)- where x and y, which may be identical or different, are chosen from integers from 1 to 4, representing a defined and unique degree of polymerization, and numbers from 1 to 4, representing an average degree of polymerization; b) a bis-secondary diamine residue such as piperazine derivatives; c) bis-primary diamine residues of formula -NH-Y-NH-, in which Y is chosen from linear or branched hydrocarbon groups and the divalent group -CH2-CH-SS-CH-CH2-CH-. and d) ureylene groups of formula -NH-CO-NH-. In at least one embodiment, X- is an anion such as chloride or bromide. Polymers of this type are described, for example, in French patents Nos. 2,320,330; 2,270,846; 2,316,271; 2,336,434; and 2,413,907 and in US patents Nos. 2,273,780; 2,375,853; 2,388,614; 2,454,547; 3,206,462; 2,261,002; 2,271,378; 3,874,870; 4,001,432; 3,929,990; 3,966,904; 4,005,193; 4,025,617; 4,025,627; 4,025,653; 4,026,945 and 4,027,020. Non-limiting examples of these polymers include those comprising at least one repeating motif of formula (III): Rin Ra + ï | : ue Ne Ge Nm Ode xx ï Reis Ris in which R13, R14, Ry5 and R16, which may be identical or different, are chosen from alkyl and hydroxyalkyl groups comprising 1 to 4 carbon atoms, n and p, which may be identical or different, are integers from 2 to 20, and X- is an anion derived from an inorganic or organic acid. (11) polyquaternary ammonium polymers comprising motifs of formula (IV): Ras av) Rio f ! È — NLOH—NH-C0-—L0H 3" 00-—MH=L0 Hate Ne A x | Ris Rn in which: Ris, Rio, Rao and R>,, which may be identical or different, are chosen from the hydrogen, methyl, ethyl, propyl, β-hydroxyethyl, β-hydroxypropyl, -CH2CH.(OCH;CH»),OH groups, where p is chosen from integers between 0 and 6, provided that Ryg, R19, Rap and R» are not simultaneously hydrogen, ret s, which can be identical or different, are chosen from integers ranging from 1 to 6, q is chosen from integers ranging from 0 to 34, - X- denotes an anion, such as a halide and A is chosen from among the radicals of dihalides and -CH--CH--O-CH,-CH,-. These compounds are described, for example, in European patent application No. 0 122 324. (12) Quaternary polymers of vinylpyrrolidone and vinylimidazole. Other examples of suitable cationic polymers include, notably, cationic proteins and cationic protein hydrolysates, polyalkyleneimines, such as polyethyleneimines, polymers comprising motifs selected from vinylpyridine and vinylpyridinium, polyamine and epichlorohydrin condensates, quaternary polyureylenes, and chitin derivatives. According to one embodiment of the present invention, (a) at least one cationic polymer is selected from cellulose ether derivatives comprising quaternary ammonium groups, such as products sold under the name "JR 400" by UNION CARBIDE CORPORATION, cationic cyclopolymers, for example, dimethyldiallylammonium chloride homopolymers and copolymers sold under the names MERQUAT® 100, MERQUAT® 550, and MERQUATE® S by CALGON, guar gums modified with a salt of 2,3-epoxypropyltrimethylammonium and the quaternary polymers of vinylpyrrolidone and vinylimidazole. (13) Polyamines As a (a) cationic polymer, it is also possible to use (co)polyamines, which can be homopolymers or copolymers, with a A plurality of amino groups. The amino group can be a primary, secondary, tertiary, or quaternary amino group. The amino group can be present in the backbone of a polymer or in a pendant group, if present, of (co)polyamines. Examples of (co)polyamines include chitosans, (co)polylamines, (co)polyvinylamines, (co)polyanilines, (co)polyvinylimidazoles, (co)polydimethylaminoethylene methacrylates, (co)polyvinylpyridines such as (co)poly-1-methyl-2-vinylpyridines, and (co)polyimines such as... (co)polyethyleneimines, (co)polypyridines such as (co)poly(quaternary pyridines), (co)polybiguanides such as (co)polyaminopropyl biguanides, (co)polylysines, (co)polyornithines, (co)polyarginines, (co)polyhistidines, amino-dextrans, aminocelluloses, amino(co)polyvinylacetals and their salts. As with (co)polyamines, it is preferable to use (co)polylysines. Polylysine is well-known. Polylysine can be a natural homopolymer of L-lysine, which can be produced by bacterial fermentation. For example, polylysine can be ε-Poly-L-lysine, commonly used as a natural preservative in food products. Polylysine is a polyelectrolyte that is soluble in polar solvents such as water, propylene glycol, and glycerol. Polylysine is commercially available in various forms, such as poly-D-lysine and poly-L-lysine. Polylysine may be available as a salt and / or a solution. An arginine / lysine polypeptide can also be used. (14) Cationic amino acids As a cationic polymer, it may be possible to use cationic polyamino acids, which can be homopolymers or cationic copolymers, with a plurality of amino and carboxyl groups. The amino group may be primary, secondary, tertiary, or quaternary. The amino group may be present in the backbone of a polymer or in a pendant group, if present, of the cationic polyamino acids. The carboxyl group may be present in a pendant group, if present, of the cationic polyamino acids. Examples of cationic polyamino acids include cationized collagen, cationized gelatin, hydroxypropyl steardimonium hydrolyzed wheat protein, hydroxypropyl cocodimonium hydrolyzed wheat protein, hydroxypropyltrimonium hydrolyzed conchiolin protein, hydroxypropyltrimonium steardimonium hydrolyzed soy protein, hydroxypropyltrimonium hydrolyzed soy protein, cocodimonium hydrolyzed soy protein, and similar products. (15) Cationic starches Cationic starches can also be cited as (a) cationic polymers. Examples of cationic starches include modified starches with a salt of 2,3-epoxypropyltrimethylammonium (ex: chloride), such as the product called starch hydroxypropyltrimonium chloride according to INCI nomenclature and sold under the name SENSOMER CI-50 by Ondeo or PencareTM DP 1015 by Ingredion. (16) Cationic gum Cationic gums can also be cited as (a) cationic polymers. Gums can, for example, be chosen from the group consisting of cassia gum, karaya gum, konjac gum, tragacanth gum, tara gum, acacia gum and gum arabic. Examples of cationic gums include cationic polygalactomannan derivatives such as guar gum derivatives and cassia gum derivatives, for example CTFA: Guar Hydroxypropyltrimonium Chloride, Hydroxypropyl Guar Hydroxypropyltrimonium Chloride, and Cassia Hydroxypropyltrimonium Chloride. Guar hydroxypropyltrimonium chloride is commercially available under the Jaguar™ brand names of Rhodia Inc. and the N-Hance brand names of Ashland Inc. Cassia hydroxypropyltrimonium chloride is commercially available under the Sensomer™ CT-250 and Sensomer™ CT-400 brands of Lubrizol Advanced Materials, Inc. or ClearHance™ of Ashland Inc. The following descriptions relate to preferred embodiments of (a) cationic polymer. It may be preferable that (a) the cationic polymer be chosen from the group consisting of alkyldiallylamine cyclopolymers and dialkyldiallylammonium cyclopolymers such as (co)polydiallyldialkylammonium chloride, (co)polyamines such as (co)polylysines, cationic (co)polyamino acids such as cationized collagen and an arginine / lysine polypeptide, cationic cellulose polymers, and their salts. It may be more preferable for the (a) cationic polymer to be chosen from the group consisting of polylysines such as poly-a-lysine and poly-e-lysine, an arginine / lysine polypeptide, polyquaternium-4, polyquaternium-10, polyquaternium-24, polyquaternium-67, starch hydroxypropyltrimonium chloride, cassia hydroxypropyltrimonium chloride, chitosans such as chitosan and a mixture thereof. It may be even more preferable that the (a) cationic polymer be chosen from polylysines such as poly-a-lysine and poly-e-lysine, an arginine / lysine polypeptide, chitosans such as chitosan, and a mixture of these. The weight ratio of the quantity of the (a) cationic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention is 0.06 to 0.25. The weight ratio of the quantity of the (a) cationic polymer(s) relative to the total weight of nanoparticles in the composition according to the present invention can be from 0.06 to 0.24, preferably from 0.07 to 0.22, and more preferably from 0.08 to 0.20. (Anionic polymer) The composition according to the present invention comprises: (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives; and (b-2) at least a second anionic polymer that is different from the (b-1) first anionic polymer. Two or more different types of anionic polymers can be used in combination for each of the (b-1) first anionic polymer and (b-2) second anionic polymer. Thus, a single type of anionic polymer or a combination of different types of anionic polymers can be used for each of the (b-1) first anionic polymer and (b-2) second anionic polymer. The (b-1) first anionic polymer and the (b-2) second anionic polymer have a negative charge density. The charge density of each of the (b-1) first anionic polymer and (b-2) second anionic polymer can be between 0.1 meq / g and 20 meq / g, preferably between 1 and 15 meq / g, and more preferably between 4 and 10 meq / g First anionic polymer: According to the present invention, the (b-1) first anionic polymer is selected from hyaluronic acids and their derivatives. Hyaluronic acid can be represented by the following chemical formula. OH OH o Ai! SES “eee —a—0 16 OH A OF 10 In the context of the present invention, the term "hyaluronic acid" specifically covers the basic hyaluronic acid motif of formula: GG Patti ilitl TC TOR CC VASL CC 1 ALICE LS CLUTODIQUE CC CHAQH SOON} ps 9 SH °: \ 2 H 5 + ; = He Le CH Ht 5 This refers to the smallest fraction of hyaluronic acid comprising a disaccharide dimer, namely D-glucuronic acid and N-acetylglucosamine. The term "hyaluronic acid and its derivatives" also includes, in the context of the present invention, a linear polymer comprising the polymeric motif described above, linked together in the chain via alternating (1,4) and B(1,3) glycosidic linkages, having a molecular weight (MW) that can range from 380 to 13,000,000 daltons. Unless otherwise defined in the descriptions, "molecular weight" means an average molecular weight by weight. This molecular weight depends largely on the source from which the hyaluronic acid is obtained and / or the preparation processes. The term "hyaluronic acid and its derivatives" also includes, in the context of the present invention, hyaluronic acid salts. Salts may include alkali metal salts such as sodium and potassium salts, alkaline earth metal salts such as magnesium salts, ammonium salts, and mixtures thereof. In its natural state, hyaluronic acid is present in pericellular gels, in the basic substance of connective tissues of vertebrate organs such as the dermis and epithelial tissues and, in particular, in the epidermis, in the synovial fluid of joints, in the vitreous humor, in the human umbilical cord and in the crista galli apophysis. Thus, the term "hyaluronic acid and its derivatives" includes all fractions or subunits of hyaluronic acid having a molecular weight in particular within the molecular weight range mentioned above. In the context of the present invention, preferably hyaluronic acid fractions that do not have inflammatory activity are used. As an illustration of the different fractions of hyaluronic acid, reference can be made to the document "Hyaluronan fragments: an information-rich system", R. Stern et al, European Journal of Cell Biology 58 (2006) 699-715, which reviews the listed biological activities of hyaluronic acid according to its molecular weight. The molecular weight of the (b-1) first anionic polymer, i.e. hyaluronic acid or a derivative thereof, may be less than or equal to 2000 kDa, preferably less than or equal to 1000 kDa and, more preferably, less than or equal to 100 kDa. The molecular weight of the (b-1)first anionic polymer, i.e. hyaluronic acid or a derivative thereof, may be 0.4 kDa or more, preferably 1 kDa or more and, more preferably, 10 kDa or more. The molecular weight of the (b-1) first anionic polymer, i.e., hya- luronique or a derivative thereof, can be from 0.4 to 2000 kDa, preferably from 1 to 1000 kDa, and more preferably from 10 to 100 kDa. Finally, the term "hyaluronic acid and its derivatives" also includes hyaluronic acid esters, in particular those in which all or part of the carboxylic groups of the acid functions are esterified with oxyethylened alkyls or alcohols, containing from 1 to 20 carbon atoms, notably with a degree of substitution at the level of D-glucuronic acid of hyaluronic acid of between 0.5 and 50%. Mention can be made, in particular, of the methyl, ethyl, n-propyl, n-pentyl, benzyl, and dodecyl esters of hyaluronic acid. These esters were notably described in D. Campoccia et al., "Semisynthetic resorbable materials from hyaluronan esterification," Biomaterials 19 (1998) 2101-2127. The hyaluronic acid derivative may be, for example, acetylated hyaluronic acid or a salt thereof such as sodium acetylated hyaluronate. The molecular weights indicated above are also valid for hyaluronic acid esters. The weight ratio of the quantity of the (b-1) first anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention may be 0.01 or more, preferably 0.02 or more, and more preferably 0.03 or more. The weight ratio of the quantity of the (b-1) first anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention may be 0.8 or less, preferably 0.7 or less, and more preferably 0.6 or less. The weight ratio of the quantity of the (b-1) first anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention can be from 0.01 to 0.8, preferably from 0.02 to 0.7, and more preferably from 0.03 to 0.6. Second anionic polymer: The (b-2) second anionic polymer is different from the (b-1) first anionic polymer. The molecular weight of the (b-2) second cationic polymer may be equal to or greater than 1,000, preferably equal to or greater than 10,000, more preferably equal to or greater than 50,000, and even more preferably equal to or greater than 100,000. Unless otherwise defined in the descriptions, "molecular weight" means an average molecular weight by weight. The (b-2) second anionic polymer may have at least one negatively chargeable and / or negatively charged fraction selected from the group composed of a group sulfuric, a sulfate group, a sulfonic group, a sulfonate group, a phosphoric group, a phosphate group, a phosphonic group, a phosphonate group, a carboxylic group and a carboxylate group. The (b-2) second anionic polymer can be a homopolymer or a copolymer. By "copolymer" is meant both copolymers obtained from two kinds of monomers and those obtained from more than two kinds of monomers, such as terpolymers obtained from three kinds of monomers. The (b-2) second anionic polymer can be chosen from natural and synthetic anionic polymers. The (b-2) second anionic polymer may comprise at least one hydrophobic chain. The (b-2) second anionic polymer, which may include at least one hydrophobic chain, may be obtained by copolymerization of a monomer (a) selected from carboxylic acids comprising an ε,P-ethylenic unsaturation (monomer a') and a 2-acrylamido-2-methylpropanesulfonic acid (monomer a") with a surface-inactive monomer (b) comprising an ethyl unsaturation other than (a) and / or a monomer (c) comprising an ethyl unsaturation resulting from the reaction of an acrylic monomer comprising an ε,8-monoethylenic unsaturation or of a monomer comprising a monoethylenic unsaturation with a nonionic amphiphilic monohydric component or with a primary or secondary amine. Thus, the anionic polymer with at least one hydrophobic chain can be obtained by two synthetic routes: - either by copolymerization of monomers (a') and (c), or (a'), (b) and (c), or (a") and (c), or (a"), (b) and (c), - either by modification (and in particular by esterification or amidation) of a co-polymer formed from monomers (a) or monomers (a"") and (b), or (a) and (b), by a non-ionic amphiphilic monohydric compound or a primary or secondary fatty amine. Reference may be made, in particular, to 2-acrylamido-2-methylpropanesulfonic acid copolymers, to those disclosed in the article "Micelle formation of random copolymers of sodium 2-(acrylamido)-2-methylpropanesulfonate and nonionic surfactant macromonomer in water as studied by fluorescence and dynamic light scattering — Macromolecules, 2000, Vol. 33, No. 10 — 3694-3704" and in applications EP-A-0 750 899 and EP-A-1 069 172. The carboxylic acid comprising an α,8-monoethylenic unsaturation constituting the monomer (α') can be chosen from many acids, including acrylic acid, methacrylic acid, crotonic acid, itaconic acid, and maleic acid. Preferably, it is acrylic or methacrylic acid. The copolymer may include a (b) monomer comprising a monoethylenic unsaturation that has no surfactant properties. Preferred monomers are those that give water-insoluble polymers when homopolymerized. These may be selected, for example, from alkyl acrylates and C,-C3 methacrylates, such as methyl acrylate, ethyl acrylate, butyl acrylate, or the corresponding methacrylates. The most preferred monomers are methyl acrylate and ethyl acrylate. Other monomers that may be used include, for example, styrene, vinyltoluene, vinyl acetate, acrylonitrile, and vinylidene chloride. Non-reactive monomers are preferred, these monomers being those in which the single ethylenic group is the only reactive group under the polymerization conditions.However, monomers that include groups that react under the effect of heat, such as hydroxyethyl acrylate, may be used optionally. Monomer (c) is obtained by reaction of an acrylic monomer comprising the α,β-monoethylenic unsaturation, such as (a), or of an isocyanate monomer comprising a monoethylenic unsaturation with a nonionic monohydric amphiphilic compound or a primary or secondary fatty amine. Nonionic monohydric amphiphilic compounds or primary or secondary fatty amines used to produce the nonionic monomer (c) are well known. Nonionic monohydric amphiphilic compounds are generally hydrophobic alkoxylated compounds comprising an alkylene oxide forming the hydrophilic portion of the molecule. The hydrophobic compounds are generally composed of an aliphatic alcohol or an alkylphenol, in which a carbon chain comprising at least six carbon atoms constitutes the hydrophobic portion of the amphiphilic compound. Preferred non-ionic monohydric amphiphilic compounds are compounds corresponding to the following formula (V): R-(OCH,CHR'),-(OCH-CH'),-OH (V) in which R is chosen from alkyl or alkylene groups comprising 6 to 30 carbon atoms and alkylaryl groups having alkyl radicals comprising 8 to 30 carbon atoms, R' is chosen from alkyl groups comprising 1 to 4 carbon atoms, n is an average number from about 1 to 150 and m is an average number from about 0 to 50, provided that n is at least as large as m. Preferably, in compounds of formula (V), the R group is chosen from alkyl groups comprising 12 to 26 carbon atoms and alkylphenyl groups in which the alkyl group is C;-C;3; the R' group is the methyl group; m=0 and n=1a2s Preferred primary and secondary fatty amines consist of one or two alkyl chains comprising 6 to 30 carbon atoms. The monomer used to form the nonionic urethane monomer (c) can be chosen from a wide variety of compounds. Any compound containing a copolymerizable unsaturation, such as an acrylic, methacrylic, or allylic unsaturation, can be used. Monomer (c) can be obtained, in particular, from an isocyanate containing a monoethylenic unsaturation, such as, especially, α,α-dimethyl-μ-isopropenylbenzyl isocyanate. Monomer (c) may be selected, in particular, from among the acrylates, methacrylates or oxyethyl alcohol itaconates (1 to 50 EO) C5-Cyy, such as steareth-20 methacrylate, oxyethyl behenyl methacrylate (25 EO), oxyethylenic monocetyl itaconate (20 EO), oxyethylethyl monostearyl itaconate (20 EO) or polyoxyethylenic alcohol-modified acrylate (25 EO) Cy2-C1 and from the dimethyl-m-isopropenylbenzyl isocyanates of oxyethylenated alcohol (1 to 50 EO) Cs-Cx, such, in particular, dimethyl-m-isopropenylbenzyl isocyanate of oxyethylenated behenyl alcohol. According to a specific embodiment of the present invention, the (b-2) second anionic polymer is selected from acrylic terpolymers obtained from (a) a carboxylic acid comprising a ε,B-ethylenic unsaturation, (b) a surface-nonactive monomer comprising an ethylenic unsaturation other than (a), and (c) a nonionic urethane monomer that is the reaction product of a nonionic monohydric amphiphilic compound with an isocyanate comprising a monoethylenic unsaturation. Reference may be made, in particular, to the following compounds as anionic polymers comprising at least one hydrophobic chain: acrylic acid / ethyl acrylate / alkyl acrylate terpolymer, such as the product in the form of a 30% aqueous dispersion marketed under the name Acusol 823 by Rohm & Haas; acrylates / steareth-20 methacrylate copolymer, such as the product marketed under the name Aculyn 22 by Rohm & Haas; (meth)acrylic acid / ethyl acrylate / oxyethylenated behenyl methacrylate (25 EO) terpopolymer, such as the product in the form of an aqueous emulsion marketed under the name Aculyn-28 by Rohm & Haas; acrylic acid / oxyethylenated monocetyl itaconate copolymer (20 EO), such as the product in the form of a 30% aqueous dispersion marketed under the name Structure 3001 by National Starch;acrylic acid / oxyethylenated monostearyl itaconate co-polymer (20°EO), such as the product in the form of a 30% aqueous dispersion marketed under the name Structure 2001 by National Starch; acrylates / acrylate modified by polyoxyethyl alcohol copolymer (25 EO) C,>-C;,, such as 30-32% copolymer latex com-; marketed under the name Synthalen W2000 by 3V SA; or methacrylic acid / methyl acrylate / dimethyl-isopropenylbenzyl isocyanate terpolymer of ethoxylated behenyl alcohol, as produced in the form of a 24% aqueous dispersion and comprising 40 ethylene oxide groups disclosed in document EP-A-0 173 109. The (b-2) second anionic polymers can also be Polyester-5, such as the product marketed as Eastman AQTM 55S Polymer by EASTMAN CHEMICAL corresponding to a chemical formula below. HO-GAGAGAGAGAGAGAG-AÀ-G-OH SO-Na*t SO,Na*+ To: dicarboxylic acid fraction G: glycol fraction SOyNat: sodium sulfo group OH: hydroxyl group It may be preferable that the (b-2) second anionic polymer be chosen from the group consisting of polysaccharides such as a carrageenan (e.g., 1-carrageenan, and A-carrageenan), pectin, alginic acid (algin), and cellulosic polymers (e.g., carboxymethylcellulose), anionic (co)polyamine acids such as (co)polyglutamic acids, (co)poly(meth)acrylic acids, (co)polyamic acids, (co)polystyrene sulfonate, (co)poly(vinyl sulfate), dextran sulfate, chondroitin sulfate, (co)polymaleic acids, (co)polyfumaric acids, (co)polymers of maleic acid, and their salts. The maleic acid copolymer may comprise one or more maleic acid comonomers, and one or more comonomers selected from vinyl acetate, vinyl alcohol, vinylpyrrolidone, olefins comprising 2 to 20 carbon atoms, and styrene. The term "maleic acid copolymer" means any polymer obtained by copolymerizing one or more maleic acid comonomers with one or more comomers selected from vinyl acetate, vinyl alcohol, vinylpyrrolidone, olefins comprising 2 to 20 carbon atoms, such as octadecene, ethylene, isobutylene, diisobutylene, or isooctylene, and styrene, the maleic acid comonomers optionally being partially or completely hydrolyzed. Hydrophilic polymers, namely polymers having a water solubility greater than or equal to 2 g / L, are preferred. In an advantageous aspect of the present invention, the maleic acid copolymer may have a mole fraction of maleic acid motifs between 0.1 and 1, and preferably between 0.4 and 0.9. The average molar mass by weight of the maleic acid copolymer can be between 1,000 and 500,000 and preferably between 1,000 and 50,000. It is preferable that the maleic acid copolymer be a styrene / maleic acid copolymer and, more preferably, a styrene / maleic acid copolymer. A 50 / 50 copolymer of styrene and maleic acid is preferably used. For example, one can use the styrene / maleic acid copolymer (50 / 50), in the form of a 30% ammonium salt in water, marketed under the reference SMA1000H® by Cray Valley or the styrene / maleic acid copolymer (50 / 50), in the form of a 40% sodium salt in water, marketed under the reference SMA1000HNa® by Cray Valley. It may be preferable that the (b-2) second anionic polymer be chosen from the group consisting of carrageenans such as t-carrageenan and A-carrageenan, algin, chondroitin sulfate, pectin and a mixture of these. It may be preferable for the (b-2) second anionic polymer to be chosen from the group consisting of carrageenan, pectin and a mixture thereof. The weight ratio of the quantity of the (b-2) second anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention is 0.26 or more, preferably 0.28 or more, and more preferably 0.30 or more. The weight ratio of the quantity of the (b-2) second anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention may be 0.9 or less, preferably 0.8 or less, and more preferably 0.7 or less. The weight ratio of the quantity of the (b-2) second anionic polymer(s) to the total weight of the nanoparticles in the composition according to the present invention can be from 0.26 to 0.9, preferably from 0.28 to 0.8, and more preferably from 0.30 to 0.7. (Non-polymer acid) The composition according to the present invention includes (c) at least one non-polymeric acid or salt(s) thereof. Two or more different types of non-polymeric acids or their salts may be used in combination. Thus, a single type of non-polymeric acid or a salt thereof, or a combination of different types of non-polymeric acids or their salts, may be used. The (c) non-polymeric acid(s) or salt(s) thereof may be chosen from monovalent non-polymeric acids and their salts, polyvalent non-polymeric acids (for example, divalent or trivalent) and their salts, and mixtures thereof. Monovalent polymeric acids can have a single pKa value. In contrast, polyvalent non-polymeric acids can have two or more pKa values. The pKa value (acid dissociation constant) is well known to those skilled in the art and should be determined at a constant temperature such as 25 °C. The (c) non-polymeric acid or its salt(s) may be included in the nanoparticles, which may be a polyionic complex. The polyvalent non-polymeric acid or its salt(s) may function as a crosslinker for the (a) cationic polymer, or the (a) cationic polymer and the (b-1) first anionic polymer and / or the (b-2) second anionic polymer. The term "non-polymeric" here means that the acid is not obtained by polymerizing two or more monomers. Therefore, a non-polymeric acid is not the same as an acid obtained by polymerizing two or more monomers, such as a polycarboxylic acid. Preferably the molecular weight of (c) non-polymeric acid or salt(s) thereof should be less than or equal to 1,000, preferably less than or equal to 800 and, more preferably, less than or equal to 700. The term "salt" here refers to a salt formed by adding suitable base(s) to a non-polymeric acid, which can be obtained from a reaction of the non-polymeric acid with the base(s) according to processes known to those skilled in the art. Examples of salts include metal salts, for example, alkali metal salts such as sodium and potassium, alkaline earth metal salts such as magnesium and calcium, and ammonium salts. (c) nonpolymeric acid or a salt (or salts) thereof may be an organic acid or salt(s) thereof, and preferably a hydrophilic or water-soluble organic acid or a salt (or salts) thereof. The monovalent non-polymer acid has a single acid group which can be chosen from the group consisting of a carboxylic group, a sulfuric group, a sulfonic group, a phosphoric group, a phosphonic group or a phenolic hydroxyl group. The nonpolymer polyvalent acid may have at least two acid groups chosen from the group consisting of a carboxylic group, a sulfuric group, a sulfonic group, a phosphoric group, a phosphonic group, a phenolic hydroxyl group and a mixture of these. The monovalent non-polymeric acid can preferably be chosen from among the monovalent carboxylic acids. In one embodiment, the monovalent non-polymer acid can be chosen from among the hydroxyl acids, and preferably alpha-hydroxy acids. Examples of alpha-hydroxy acids include lactic acid and glycolic acid. The polyvalent non-polymer acid or salt thereof may be selected from the group consisting of oxalic acid, malonic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, suberic acid, azelaic acid, sebacic acid, fumaric acid, maleic acid, malic acid, citric acid, aconitic acid, oxaloacetic acid, tartaric acid and their salts; aspartic acid, glutamic acid and their salts; sulfonic acid terephthalylidene diamphr or their salts (Mexoryl SX), Benzophenone-9; phytic acid and its salts;Red 2 (Amaranth), Red 102 (New Coccine), Yellow 5 (Tartrazine), Yellow 6 (Sunset Yellow FCF), Green 3 (Fast Green FCF), Blue 1 (Brillant blue FCF), Blue 2 (Indigo Carmine), Red 202 (Lithol Rubine B), (Lithol Rubine BCA), Red 204 (Lake Red CBA), Red 206 (Lithol Red CA), Red 207 (Lithol Red BA), Red 208 (Lithol Red SR), Red 219 (Brilliant Lake Red R), Red 220 (Deep Maroon), Red 227 (Fast Acid Magenta), Yellow 203 (Quinoline Yellow WS), Green 201 (Alizanine Cyanine Green F), Green 204 (Pyranine Conc), Green 205 (Light Green SF Yellowish), Blue 203 (Patent Blue CA), Blue 205 (Alfazurine FG), Red 401 (Violamine R), Red 405 (Permanent Re FSR), Red 502 (Ponceau 3R), Red 503 (Ponceau R), Red 504 (Ponceau SX), Green 401 (Naphthol Green B), Green 402 (Guinea Green B), and Black 401 (Naphthol Blue Black); acide folique, acide ascorbique, acide erythorbique et leurs sels ; cystine et ses sels ; EDTA et ses sels ; glycyrrhizine et ses sels ; et un mélange de ceux-ci. ; It may be preferable that the polyvalent non-polymer acid or a salt thereof be chosen from the group consisting of terephthalylidene diamphr sulfonic acid and its salts (Mexoryl SX), Yellow 6 (Sunset Yellow FCF), ascorbic acid, phytic acid and its salts, and a mixture of these. (c) nonpolymeric acid or a salt thereof may be selected from the group consisting of phytic acid, citric acid, lactic acid and a mixture thereof. The weight ratio of the quantity of the (c) non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles in the composition according to the present invention is 0.03 or more, preferably 0.035 or more, and more preferably 0.04 or more. The weight ratio of the quantity of the (c) non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles in the composition according to the present invention may be 0.6 or less, preferably 0.5 or less, and more preferably 0.4 or less. The weight ratio of the quantity of the (c) non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles in the composition according to the present invention may be from 0.03 to 0.6, preferably from 0.035 to 0.5, and more preferably rentiellement from 0.04 to 0.4. [Metal salt] The composition according to the present invention may comprise (d) at least one metal salt. Two or more metal salts may be used in combination. (d) at least one metal salt is an optional ingredient. Thus, the nanoparticles may not include (d) the metal salt. The term "metal salt" here refers to a cosmetically acceptable, water-soluble salt that provides cationic ions in an aqueous medium. The metal salt used in the composition of the present invention is preferably chosen from versatile metal salts, such as divalent or trivalent metal salts, and is more preferably chosen from divalent metal salts such as calcium, magnesium, and zinc salts. Calcium salts, such as calcium chloride, calcium pyrrolidone carboxylate (PCA), calcium acetate, calcium aspartate, and calcium lactate, are preferred. Among others, calcium chloride and calcium pyrrolidone carboxylate are the most preferable. The (d) metal salt may be chosen from polyvalent metal salts, preferably divalent metal salts, more preferably calcium salts such as calcium chloride and calcium pyrrolidone carboxylate, and magnesium salts such as magnesium chloride. The weight ratio of the quantity of the (d) metal salt(s) to the total weight of the nanoparticles in the composition according to the present invention may be 0.001 or more, preferably 0.005 or more, and more preferably 0.01 or more. The weight ratio of the quantity of the metal salt(s) (d) to the total weight of the nanoparticles in the composition according to the present invention is 0.30 or less, preferably 0.25 or less, and more preferably 0.20 or less. The weight ratio of the quantity of metal salt (d) to the total weight of nanoparticles in the composition according to the present invention can be from 0.001 to 0.30, preferably from 0.005 to 0.25, and more preferably from 0.01 to 0.20. [Fatty acid] The composition according to the present invention comprises (e) at least one fatty acid. If two or more fatty acids are used, they may be identical or different. The term "fatty acid" here refers to a carboxylic acid with a long aliphatic carbon chain. The fatty acid (e) comprises at least 4 carbon atoms, preferably at least 6 carbon atoms and, more preferably, at least 8 carbon atoms. The fatty acid (e) may comprise up to 24 carbon atoms, preferably up to 22 carbon atoms and, more preferably, up to 20 carbon atoms. It is preferable that the (e) fatty acid be chosen from a C4-Cz4 fatty acid, more preferably a C,-C,2 fatty acid, and even more preferably a C,-C20 fatty acid The (e) fatty acid can be chosen from among the saturated fatty acids which can be linear or branched. Examples of saturated fatty acids include caprylic acid (C;), pelargonic acid (Cs), capric acid (Cy0), lauric acid (C;>), myristic acid (C,4), pentadecanoic acid (C;s), palmitic acid (Cy6), heptadecanoic acid (C,,), stearic acid (Cys), isostearic acid (Cys), nonadecanoic acid (C;s), arachidic acid (C»0), behenic acid (C») and lignoceric acid (C2). The (e) fatty acid can be chosen from unsaturated fatty acids which can be linear or branched. Unsaturated fatty acids, whether linear or branched, can be monounsaturated fatty acids, linear or branched, or polyunsaturated fatty acids, linear or branched. A carbon-carbon double bond or a carbon-carbon triple bond can be used as the unsaturated fraction of unsaturated fatty acids, linear or branched. Examples of unsaturated fatty acids include myristoleic acid (C;4), palmitoleic acid (Cy6), oleic acid (Cys), linoleic acid (C;5), l-inolenic acid (Cys), elaidic acid (C;s), arachidonic acid (C;p), eicosenoic acid (C0), erucic acid (C,.) and nervonic acid (C4). In a preferred embodiment, the (e) fatty acid can be selected from saturated or unsaturated, linear or branched fatty acids. Thus, the (e) fatty acid can be selected from saturated and unsaturated, linear or branched fatty acids in C1-C18, preferably C1-C18, more preferably C1-C18. The (e) fatty acid may be chosen from the group consisting of caprylic acid, capric acid, oleic acid, linoleic acid, myristic acid, stearic acid, isostearic acid, behenic acid and mixtures thereof. The fatty acid (e) may be in the form of a free acid or a salt thereof. Examples of fatty acid salts include inorganic salts such as alkali metal salts (sodium salts, potassium salts, or similar) and alkaline earth metal salts (magnesium salts, salts of salt, or similar); and organic salts such as ammonium salts (quaternary ammonium salts or similar) and amine salts (triethanolamine salts, triethylamine salts, or similar). A single type of fatty acid salt or a combination of different types of fatty acid salts may be used. Furthermore, a combination of one or more fatty acids in free acid form and one or more fatty acids in salt form may be used, in which one or more types of salts may also be employed. The weight ratio of the quantity of the fatty acid(s) to the total weight of the nanoparticles in the composition according to the present invention is 0.001 or more, preferably 0.002 or more, and more preferably 0.003 or more. The weight ratio of the quantity of the fatty acid(s) to the total weight of the nanoparticles in the composition according to the present invention may be 0.4 or less, preferably 0.3 or less, and more preferably 0.2 or less. The weight ratio of the quantity of the fatty acid(s) relative to the total weight of the nanoparticles in the composition according to the present invention can range from 0.001 to 0.4, preferably from 0.002 to 0.3, and more preferably from 0.003 to 0.2. [Water] The composition according to the present invention comprises (f) water. The quantity of (f) water in the composition according to the present invention may be greater than or equal to 50% by weight, preferably greater than or equal to 60% by weight and more preferably greater than or equal to 70% by weight, relative to the total weight of the composition. Furthermore, the quantity of (f) water in the composition according to the present invention may be less than or equal to 95% by weight, preferably less than or equal to 90% by weight and more preferably less than or equal to 85% by weight, relative to the total weight of the composition. The quantity of (f) water in the composition according to the present invention may be from 50% to 95% by weight, preferably from 60% to 90% by weight and more preferably from 70% to 85% by weight, relative to the total weight of the composition. [pH] The pH of the composition according to the present invention can be from 3 to 9, preferably from 3.3 to 8.5 and, more preferably, from 3.5 to 8. At a pH between 3 and 9, the polyionic complex formed by ingredients (a) to (e), in particular ingredients (a), (b-1), (b-2) and (c), can be very stable. The pH of the composition according to the present invention can be corrected by adding at least one alkali and / or at least one acid, other than (c) non-polymer acid or salt(s) thereof. The pH of the composition according to the present invention can also be corrected by adding at least one buffering agent. (Alkaline agent) The composition according to the present invention may comprise at least one alkali agent. Two or more alkali agents may be used in combination. Thus, a single type of alkali agent or a combination of different types of alkali agents may be used. The alkali agent may be an inorganic alkali agent. It is possible that the agent inorganic alkali is chosen from the group consisting of the following: ammonia, alkali metal hydroxides, alkaline earth metal hydroxides, alkali metal phosphates and monohydrogen phosphates such as sodium phosphate or sodium monohydrogen phosphate. Examples of inorganic alkali metal hydroxides include sodium hydroxide and potassium hydroxide. Examples of alkaline earth metal hydroxides include calcium hydroxide and magnesium hydroxide. Sodium hydroxide is the preferred inorganic alkali metal. The alkali agent may be an organic alkali agent. It is preferable that the organic alkali agent be chosen from the group consisting of monoamines and their derivatives; diamines and their derivatives; polyamines and their derivatives; basic amino acids and their derivatives; oligomers of basic amino acids and their derivatives; polymers of basic amino acids and their derivatives; urea and its derivatives; and guanidine and its derivatives. Examples of organic alkali metals include alkanolamines such as mono-, di-, and triethanolamine, and isopropanolamine; urea, guanidine, and their derivatives; basic amino acids such as lysine, ornithine, or arginine; and diamines such as those described by the structure below: R1 R3 x A N—R—N / *% R2 R4 in which R denotes an alkylene such as propylene optionally substituted by a C,-C, hydroxyl or alkyl radical, and R, Ra, R3 and R4 independently denote a hydrogen atom, an alkyl radical or a C,-C, hydroxyalkyl radical, such as may be exemplified by 1,3-propanediamine and its derivatives. Arginine, urea and monoethanolamine are preferable. The alkaline agent(s) may be used in an amount ranging from 0.01% to 15% by weight, preferably from 0.02% to 10% by weight, and more preferably from 0.03% to 5% by weight, relative to the total weight of the composition, depending on their solubility. (Acid) The composition according to the present invention may comprise at least one acid. Two or more acids may be used in combination. Thus, a single type of acid or a combination of different types of acids may be used. As for acids, we can mention all inorganic or organic acids, preferably inorganic, that are commonly used in cosmetic products. A monovalent acid and / or a polyvalent acid can be used. A monovalent acid Acids such as citric acid, phosphoric acid, and hydrochloric acid (HCl) can be used. HCl is preferred. The acid(s) may be used in an amount ranging from 0.01% to 15% by weight, preferably from 0.02% to 10% by weight, and more preferably from 0.03% to 5% by weight, relative to the total weight of the composition, depending on their solubility. (Buffering agent) The composition according to the present invention may comprise at least one buffering agent. Two or more buffering agents may be used in combination. Thus, a single type of buffering agent or a combination of different types of buffering agents may be used. Examples of buffering agents include acetate buffers (e.g., acetic acid + sodium acetate), phosphate buffers (e.g., sodium dihydrogen phosphate + disodium hydrogen phosphate), citrate buffers (e.g., citric acid + sodium citrate), borate buffers (e.g., boric acid + sodium borate), tartrate buffers (e.g., tartaric acid + sodium tartrate dihydrate), and Tris buffers (e.g., tris(hydroxymethyl)aminomethane) and a Hepes buffer (acid 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid). [Optional ingredients] The composition according to the present invention may include, in addition to the aforementioned components, at least one optional ingredient typically used in cosmetics, which may be selected from, specifically, oils, surfactants or emulsifiers, hydrophilic or lipophilic thickeners, volatile or non-volatile organic solvents such as polyols, in particular glycerin and glycols, silicones and silicone derivatives, natural extracts derived from animals or vegetables, waxes, UV filters and the like, in a range which does not affect the effects of the present invention. The composition according to the present invention may comprise the optional ingredient(s) in an amount of 0.01% to 50% by weight, preferably 0.05% to 30% by weight, and more preferably 0.1% to 10% by weight, relative to the total weight of the composition. However, it is preferable that the composition according to the present invention include a very limited amount of surfactant(s) or emulsifier(s). The amount of surfactant(s) or emulsifier(s) in the composition according to the present invention may be 1% by weight or less, preferably 0.1% by weight or less, and more preferably 0.01% by weight or less, relative to the total weight of the composition. It is particularly preferable that the composition according to the present invention not include of surfactant or emulsifier. [Composition] The composition according to the present invention can be prepared by mixing the essential ingredient(s) as explained above, and the optional ingredient(s), if necessary, as explained above. The method and means for mixing the essential and optional ingredients above are not limited. Any conventional method and means may be used to mix the essential and optional ingredients above in order to prepare the composition according to the present invention. The composition according to the present invention can be used as a cosmetic composition. Thus, the cosmetic composition according to the present invention can be intended for application to a keratin fiber. By keratin substance, we mean here a material containing keratin as its principal constituent and, by way of example, skin, scalp, lips, hair, and the like. It is preferable that the cosmetic composition according to the present invention be used for a cosmetic process on the keratin fiber, in particular the skin. The composition according to the present invention may be transparent or translucent, preferably transparent. Transparency can be measured by measuring turbidity (turbidity can be measured, for example, with a 2100Q incandescent lamp (marketed by Hach Company) equipped with a round cell (25 mm in diameter and 60 mm high) and a tungsten filament lamp capable of emitting visible light (between 400 and 800 nm, preferably 400 to 500 nm). The measurement can be performed on the undiluted composition. The blank test can be evaluated with distilled water. The composition according to the present invention may have a turbidity of 300 NTU or less, preferably 200 NTU or less, more preferably 100 NTU or less, and even more preferably 50 NTU or less. [Cosmetic process and use] The present invention also relates to: a cosmetic process for a keratinous substrate such as skin, comprising: the application to the keratinous substrate of the composition according to the present invention. The cosmetic process here refers to a non-therapeutic cosmetic process of care and / or makeup of the surface of a keratinous substance such as the skin. The cosmetic process according to the present invention can provide a keratinous substance such as skin with cosmetic effects derived from hyaluronic acid and / or a hyaluronic acid derivative. For example, the cosmetic process according to the present invention can provide a keratinous substance such as skin with moisturizing effects. The present invention may also relate to the use of (e) at least one fatty acid in a composition comprising: (a) at least one cationic polymer; (b-1) at least one first anionic polymer selected from hyaluronic acids and their derivatives (b-2) at least a second anionic polymer that is different from the (b-1) first anionic polymer; (c) at least one non-polymeric acid or a salt thereof; and (d) at least one optional metal salt; and ({) of water, in which Ingredients (a), (b-1), (b-2), (c), (d) and (e) are capable of forming nanoparticles, the quantity of which is between 0.01% and 0.58% by weight, preferably between 0.05% and 0.57% by weight, and more preferably between 0.1% and 0.56% by weight, relative to the total weight of the composition, the weight ratio of the quantity of the (a) cationic polymer(s) to the total weight of the nanoparticles is 0.06 to 0.25, the weight ratio of the quantity of the (b-2) second anionic polymer to the total weight of the nanoparticles is 0.26 or more, the weight ratio of the quantity of the non-polymer acid(s) or salt(s) thereof to the total weight of the nanoparticles is 0.03 or more, the weight ratio of the quantity of the (d) optional metal salt(s) to the total weight of the nanoparticles is 0.30 or less, and the weight ratio of the quantity of the fatty acid(s) relative to the total weight of the nanoparticles is 0.001 or more, in order to enhance the penetration capacity of nanoparticles into a keratinous substance such as skin. The above-described use can deliver hyaluronic acid and / or a hyaluronic acid derivative deeper into a keratinous substance such as skin. Therefore, the above-described use according to the present invention can provide a keratinous substance such as skin with cosmetic effects derived from hyaluronic acid and / or a hyaluronic acid derivative. For example, the use according to the present invention can provide a keratinous substance such as skin with moisturizing effects. EXAMPLES The present invention will be described in more detail with the aid of examples. However, these examples should not be interpreted as limiting the scope of the present invention. [Example 1 and Comparative Example 1] [Preparation] Each of the compositions according to Example 1 and Comparative Example 1 was prepared by mixing the ingredients listed in Table 1. The numerical values ​​of the quantities of ingredients in Table 1 are all based on a "% by weight" of active substances. The compositions according to Example 1 and Comparative Example 1 included nanoparticles containing hyaluronic acid. "NPAH" in Table 1 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 1 correspond to those of the claims. [Table 1] |Ex.1 [NPAH (b-1) |Sodium hyaluronate labeled with fluorine- 0.05 1 |&-D Fluorescence-labeled sodium hyaluronate Carrageenan Polylysine (c) |Phytic acid (d) Calcium PCA © |Oleic acid Caprylyl Glycol Glycerin Pentylene glycol Propylene glycol, polyglyceryl-2 oleate, polyglyceryl-4 caprate, water Depth of skin penetrated by hyaluronic acid (µm) 112 14 | NPAH concentration (% by weight) 0.25 0, Weight ratio of (b-1) to total NPAH weight [0202 jo. Weight ratio of (b-2) to total NPAH weight 0, Weight ratio of (a) to total NPAH weight 0, Weight ratio of (c) to total NPAH weight 0.087 in Weight ratio of (d) to total NPAH weight 0.154 0, Weight ratio of (e) to total NPAH weight 0.028 |- Appearance Good |B Particle size (nm) 40 a: [Turbidity (NTU) 2 |1 [Reviews] (Depth of skin penetrated by hyaluronic acid) Penetration testing experiments were carried out using a cell Franz diffusion (Hanson) was performed using dermatomic human skin supplied by Biopredic International (France). This apparatus consisted of a donor and a recipient portion, with dermatomic human skin sandwiched between them. The recipient portion, of a predetermined volume, was filled with a recipient solution (phosphate buffer solution, pH 7) maintained at 32°C and continuously stirred with a small magnetic stir bar. Each of the compositions according to Example 1 and Comparative Example 1 was spread with a spatula onto the membrane of the donor portion at a concentration of 30 mg / m². After 24 hours, the dermatomic human skin was removed and cryosectioned, and the depth of NPAH penetration was measured by cross-sectional observation using a fluorescence microscope. The results are shown on the line titled "Depth of skin penetrated by hyaluronic acid (yum)" in Table 1. (Appearance) The appearance of the compositions according to Example 1 and Comparative Example 1 was visually assessed at room temperature (25°C) according to the following criteria. Good: Transparent Bad: Turbid The results are shown on the line marked "Aspect" in Table 1. (Particle size) The particle size or diameter of NPAH in the compositions according to Example 1 and Comparative Example 1 was measured by an ELSZ-2000 particle size analyzer from Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown in the row titled "Particle Size (nm)" in Table |. (Turbidity) The turbidity of the compositions according to Example 1 and Comparative Example 1 was measured using a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown in the row labeled "Turbidity" in Table 1. (Summary) The experimental data shown in Table 1 show that NPAH made hydrophobic with fatty acid penetrates deeper into the skin than NPAH not made hydrophobic. Thus, the nanoparticles in the composition according to the present invention, including (e) at least one fatty acid, can penetrate deeper into the skin. [Examples 2-6 and comparative example 2] [Preparation] Each of the compositions according to examples 2 to 6 and comparative example 2 has been prepared by mixing the ingredients shown in Table 2. The numerical values ​​of the quantities of ingredients in Table 2 are all based on a "% by weight" of active ingredients, The compositions according to Examples 2 to 6 and Comparative Example 2 included particles containing hyaluronic acid. "NPAH" in Table 2 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 2 correspond to those of the claims. [Tables 2] |Ex.2 |Ex. of |Ex. 5 |Ex. 6 Ex. 4 comp. 2 |NPAH (b- |Sodium hyaluronate |0.05 |0.07 |o.09 |o.1 «Jo. 0.12 1) |(20-50 kDa) pr omgpine 01 [0.14 Jo.18 lo2 J022 J0.24 2) Phytic acid 0.051 Calcium PCA 0.090 Oleic acid 0.017 Glycerin 10 10 rer a Pi PE 5 mat Jos [0.14 jo,1s [02 |022 [024 2) [(@) |Polylysine [0.031 [0.044 [0.056 [0.063 [0.069 |0.075 Phytic acid 0.051 Calcium PCA 0.090 Oleic acid 0.017 Glycerin 10 10 Pentylene glycol ps 5 ds ps 8 ds - |Propylene glycol 5 5 5 5 5 5 Polyglyceryl oleate- 0.05 |0.05 |0.05 0.05 |0.05 0.05 2 Polyglyceryl caprate--0.45 |0.45 |0.45 |0.45 |0.45 |0.45 4 (© |Eau lasp Jap Jap asp [asp [asp 100 [0.152 [0.153 [0.152 |© [Water qsp |jqsp asp |asp |qsp |asp 100 100 |100 |100 |100 |100 [NPAH Concentration (% by weight) 0.50 0.59 Weight ratio of (b-1) to total NPAH weight ratio of (b-2) to total NPAH weight ratio of (b-2) to total NPAH weight ratio of (a) to total NPAH weight ratio of (a) to total NPAH weight ratio of (c) to total NPAH weight ratio of (c) to total NPAH weight ratio of (c) to total NPAH weight ratio of (b-2 ... weight ratio of (b-2) to total NPAH weight ratio of (b-2) weight ratio of (b-2) weight ratio of (b-2) weight ratio of (b-2) weight ratio of (b-2) weight ratio of (b- [0.086 |0.085 |0.087 [0.086 0.086 of NPAH Weight ratio of (d) to total weight|0.153 [0.153 [0.153 [0.152 [0.153 [0.152 of NPAH [Weight ratio of (e) to total weight |0.028 |0.029 [0.029 |0.028 0.028 10.029 by NPAH | Aspect [Aspect [Good [Good [Good [Good [Good [Poor | [Particle Size (nm) 324 |572 |622 |140.2 |229.3 |184.9 Turbidity (NTU) 76.6 333 [Reviews] (Appearance) The appearance of the compositions according to examples 2 to 6 and comparative example 2 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Precipitation has been observed The results are shown on the line marked "Aspect" in Table 2. (Particle size) The particle size or diameter of NPAH in the compositions according to Examples 2 to 6 and Comparative Example 2 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 2. (Turbidity) The turbidity of the compositions according to Examples 2 to 6 and Comparative Example 2 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 9. (Summary) The experimental data shown in Table 2 show that if the amount of nanoparticles is 0.59% by weight or more, relative to the total weight of the composition, precipitation occurs. [Reference Example 1 and Examples 7-11] [Preparation] Each of the compositions according to Reference Example 1 and Examples 7-11 was prepared by mixing the ingredients listed in Table 3. The numerical values ​​of the quantities of ingredients in Table 3 are all based on a "% by weight" of active substances, The compositions according to Reference Example 1 and Examples 7-11 included particles including hyaluronic acid (except for Reference Example 1). “NPAH” in Table 3 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 3 correspond to those of the claims. [Tables 3] |Re. por |Ex.8 |Ex.9 Ex. |Ex. 7 10 July | Joo1 Joos Joos jo! |o2 0.1. 0.1 0.1 0.1 0.1 |0.1 |NPAH (β- |Sodium Hyaluronate 1) (20-50 kDa) Carrageenan 2) Polylysine Phytic Acid Calcium PCA Oleic Acid Caprylyl Glycol Glycerin Pentylene glycol - J0.107 [0.101 [0.093 [0.085 |0.070 [0.053 | [0.192 [0.184 [0.167 [0.154 [0.128 [0.095 | Phytic acid 0.021 Calcium PCA 0.038 (© | Oleic acid [0.007 [0.007 [0.007 [0.007 [0.007 |0.007 | Caprylyl Glycol 0.3 0.3 Glycerin 10 |10 10 Pentylene glycol gs qe 5 qp 5 qe Propylene glycol 5 5 5 5 5 5 Polyglyceryl-2 oleate |0.05 [0.05 |005 [0.05 [0.05 0.05 Polyglyceryl-4 caprate 0.45 (© | Water asp jasp asp Jasp asp asp 100 |100 100 |100 100 |100 NPAH concentration (% by weight) 0.40 Weight ratio of (b-1) on Total weight |- | |0.048 |0.132 [0.202 [0.337 |0.504 of NPAH Weight ratio of (b-2) to total weight [0.508 [0.483 [0.440 [0.405 [0.337 [0.252 of NPAH Weight ratio of (a) to total weight 0.157 [0.150 |0.137 J0.126 [0.104 10.078 of NPAH Weight ratio of (c) to total weight “0.107 [0.101 0.093 [0.085 |0.070 0.053 | of NPAH Weight ratio of (d) to total weight [0.192 [0.184 [0.167 [0.154 [0.128 [0.095 | of NPAH Fame weight of (e) on total weight of [0.036 [0.034 0.031 [0.028 [0.024 |0.018 | NPAH Appearance Good |Good |Good |Good |Good |Good Particle size (nm) 37.2 [408 |471 [303 [39.6 512 Turbidity (NTU) 16 15.2 |14.5 |15 13.4 |27.8 [Reviews] (Appearance) The appearance of the compositions according to Reference Example 1 and Examples 7-11 was assessed visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Turbid The results are shown on the line marked "Aspect" in Table 3. (Particle size) The particle size or diameter of NPAH in the compositions according to Reference Example 1 and Examples 7-11 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co, Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 3. (Turbidity) The turbidity of the compositions according to Reference Example 1 and Examples 7-11 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 3. (Summary) The experimental data shown in Table 3 show that the nanoparticles can be prepared without depending on the amount of the (b-1) first anionic polymer. However, if no (b-1) first anionic polymer is used (Reference Example 1), the cosmetic effects derived from the (b-1) first anionic polymer cannot be achieved. [Examples 12 to 15 and Comparative Examples 3 to 6] [Preparation] Each of the compositions according to Examples 12 to 15 and Comparative Examples 3 to 6 was prepared by mixing the ingredients listed in Table 4. The numerical values ​​of the quantities of ingredients in Table 4 are all based on a "% by weight" of active substances. The compositions according to Examples 12-15 and Comparative Examples 3-6 in- included particles containing hyaluronic acid. "NPAH" in Table 4 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 4 correspond to those of the claims. [Tables 4] comp.|Ex. 12 13 -Jo.05 0.05 Jo, 1 jo. 15 Joo31 0.021 0.007 15 |5 [5 \|s -Jo.05 0.05 0.45 [0.45 (© |Water qsp sp |asp 100 100 |100 Concentration in 0.15 0.25 |0.30 NPAH (% by weight) [0.318 [0.282 [0.254 pre [0.202 pr gs pr | 8 there [0.064 0.169 [0.254 |0.405 |0.50 |0.57 067 | 5 6 1 Weight ratio of (b-1) to total NPAH weight ratio of (b-2) to total NPAH weight } |0.175 «Jo,157 / (0.126 [0.10 10.08 |0.06 4 9 9 Weight ratio of (a) 10.211 [0.187 on total weight of NPAH Weight ratio of (c) [0.143 (0.134 [0.119 [0.107 [0.085 0.07 |0.06 004 | on total weight of 1 1 7 NPAH Weight ratio of (d) [0.258 |0.242 |0.215 |0.193 |0.154 [0.12 0.11 10.08 on total weight of 8 0 5 NPAH Weight ratio of (e) [0.048 10.045 [0.04 [0.035 [0.028 0.02 |002 |001 | on total weight of 4 0 6 NPAH [Appearance [Bad [Bad [Bad [Bad [Good [Good [Good [Good | Weight ratio of (e) [0.048 [0.045 |0.04 [0.035 |0.028 pr _ er | on total weight of 4 0 6 NPAH [Aspect Bad Bad Bad Bad Bad [Good [Good [Good [Good | ssss Particle size (nm) [193.6 |129 |255.1 |46718 |324 |72 |59 |604 Turbidity (NTU) 542 |368 [902 |>1000 10.7 [Reviews] (Appearance) The appearance of the compositions according to Examples 12-15 and Comparative Examples 3-6 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Turbid The results are shown on the line marked "Aspect" in Table 4. (Particle size) The particle size or diameter of NPAH in the compositions according to Examples 12-15 and Comparative Examples 3-6 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 4. (Turbidity) The turbidity of the compositions according to Examples 12-15 and Comparative Examples 3-6 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 4. (Summary) The experimental data shown in Table 4 show that if the weight ratio of the amount of the (b-2) second anionic polymer, relative to the total weight of the nanoparticles, is less than or equal to 0.254, the appearance of a composition including the nanoparticles becomes turbid (not transparent). [Examples 16 to 20 and Comparative Examples 7 to 9] [Preparation] Each of the compositions according to Examples 16 to 20 and Comparative Examples 7 to 9 was prepared by mixing the ingredients listed in Table 5. The numerical values ​​of the quantities of ingredients in Table 5 are all based on a "% by weight" of active ingredients. The compositions according to Examples 16-20 and Comparative Examples 7-9 included particles containing hyaluronic acid. "NPAH" in Table 5 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 5 correspond to those of the claims. [Tables 5] 'Jo.05 Joos |oos |oos Jo.05 |0.05 NPA H Jo: for Jos [o1 jo.1 jo: | 0.020 [0.031 [0.038 [0.045 |0.050 0.075 And. comp. Jcomp. JEx. |Ex. |Ex. |Ex. comp. 16 17 18 19 20 Ex.9 7 (b- |Hyaluronate |0.05 Jos 0.05 |0.05 |0.05 |0.05 |0.05 1) |of sodium (20-50 kDa) | jme 0.1 eq.1 01 E: Eq1 Eq1 Eq.1 2) (a) |Polylysine | 0.020 [0.031 [0.038 |0.045 [0.050 [0.075 0.021 0.021 [0.021 [0.021 |0.021 |0.021 |0.021 10.038 0.038 10.038 [0.038 |0.038 |0.038 0.007 0.007 10.007 |0.007 |0.007 |0.007 |0.007 0.3 03 03 [0.3 10.3 0.3 [0.3 kings fie pre po pe 56 6566 |s 5 [5 |5 |5 |s \|s 'I,05 Jo,os Joos |o,os Jo.05 |o,0 Caprate of |0.45 045 |o4s |o.45 |o,4s Jo4s |o,45 polyglyceryl 4 © Eau |asp asp jasp Jap Jesp asp |asp qsp jqasp [asp |asp asp asp 100 |I00 |100 |100 |100 |100 0.24 |0.25 [0.25 [0.26 |0.27 |0.29 [© |Water qsp 100 NPAH concentration |0.22 (wt%) Weight ratio of (b-1) [0.232 0.212 10.202 |0.197 |0.192 |0.188 |0.172 on total weight of NPAH Weight ratio | |0.463 [0.437 (0.424 [405 [0.394 [0.383 [0.376 [0.344 (b-2) on total NPAH weight ratio of (a) |- 0.057 |0.085 [0.126 [0.149 |0.172 [0.188 [0.258 on total NPAH weight ratio of (c) |0.097 |0.092 |0.090 [0.085 0.083 [0.080 0.079 |0.072 on total NPAH weight ratio of (d) |0.176 |0.166 0.159 [0.154 10.149 10.146 [0.143 |0.130 on total NPAH weight ratio in weight of (e) |0.032 |0.030 |0.030 [0.028 |0.028 [0.027 10.026 [0.024 on total weight of NPAH |Appearance |Good [Good [Good [Good [Good [Good [Good [Good |Bad! |Good |Good |Good [Good |Good |Good |Good |Good [Particle size (nm) [ND [ND 107.5 [53.3 [05 [Good |Good near s [172.3 [172.3 [885.7 Turbidity (NTU) [063 [73 |496 [26 [928 |254 [72 |>1000 | [Reviews] (Appearance) The appearance of the compositions according to Examples 16-20 and Comparative Examples 7-9 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Precipitation has been observed The results are shown on the line marked "Aspect" in Table 5. (Particle size) The particle size or diameter of NPAH in the compositions according to Examples 16 to 20 and Comparative Examples 7 to 9 were measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 5. ND (not available) in Table 5 means that no nanoparticles were found. (Turbidity) The turbidity of the compositions according to Examples 16-20 and Comparative Examples 7-9 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table S. (Summary) The experimental data shown in Table 5 show that if the weight ratio of the amount of (a) cationic polymer, relative to the total weight of the particles, is 0.057 or less, the particles cannot have a nanometric size (less than 1000 nm), and that if the weight ratio of the amount of (a) cationic polymer, relative to the total weight of the particles, is 0.258 or more, precipitates form and the turbidity of a composition including the particles becomes too high. [Examples 21 to 26 and Comparative Examples 10 to 11] [Preparation] Each of the compositions according to Examples 21 to 26 and Comparative Examples 10 to 11 was prepared by mixing the ingredients listed in Table 6. The numerical values ​​of the quantities of ingredients in Table 6 are all based on a "% by weight" of active substances. The compositions according to Examples 21-26 and Comparative Examples 10-11 included particles containing hyaluronic acid. "NPAH" in Table 6 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 6 correspond to those of the claims. 0.031 [0.031 |0.031 [0.031 [0.031 [0.031 |0.031 |0.031 - [Tables6] Ex. Comp.|comp |Ex. |Ex. |comp |comp |comp. Ex.10 | Ex. |.21 22 23 .24 |.25 |26 11 NPA |(b-|Hyaluronate |0.05 0.05 |0.05 |0.05 [0.05 |0.05 |0.05 |0.05 H 1) |sodium (20-50 kDa) me 0.1 jo. “Jo. 01 [o.1 [o.1 0.1 lo, 2) I (a) Polylysine [0.031 |0.031 [0.031 [0.031 [0.031 |0.031 [0.031 |0.031 0.007 “0.007 0.007 |0.007 [0.007 [0.007 |0.007 |0.007 0.3 03 (0.3 0.3 0.3 03 0.3 10.3 5 5 |j5 |5 |s |s |s \|s © Acide |- [0.005 [0.010 [0.021 [0.030 [0.040 [0.050 [0.100 phytique | PCAde [0038 |0,038 |0,038 [0,038 [0,038 [0,038 [0.038 [0,038 calcium |) Acide [0.007 [0.007 |o,007 [0,007 [0,007 [0,007 0,007 [0,007 oléique Caprylyl Jos Jos |os Jos Jos Jos Jos Jos Glycol Pentylène |s |s |s |s |s |s |s |s glycol Propylène |5 5 |5 | | |5 | | glycol Oléate de pop 0,05 lo,05 |o,05 Jo,o5 |o,05 |o,05 |0,05 lyglycéryle-2 Capratede [0.45 0.45 |0.45 [0.45 |0.45 |0.45 |0.45 |0.45 polyglycéryle 4 [4 © [Eau [asp 100 asp [asp asp [asp [asp asp [asp 100 100 |100 |100 |100 |100 [100 Concentration en NPAH |0,23 0,23 |0,24 10,25 10,26 |0,27 [0,28 |0,33 (% en poids) [0.221 0.216 0.212 [0.202 [0.196 |0.188 [0.181 [0.153 0.443 |0.433 10.424 10.405 [0.391 |0.376 [0.363 10.307 Weight ratio of (b-1) to total NPAH weight ratio of (b-2) to total NPAH weight -J0.134 0.131 [0.126 0.121 |0.117 0.112 [0.095 Weight ratio of (a) |0.137 to total weight of NPAH [0.081 [0.030 [0.030 [0.028 [0.027 [0.026 [0.025 [0.021 Weight ratio of (c) | (0.022 [0.042 |0.085 [0.117 [0.150 [0.181 [0.307 on total weight of NPAH Weight ratio of (d) [0.168 |0.165 |0.161 |0.154 0.148 |0.143 [0.138 |0.117 on total weight of NPAH Weight ratio of (e) [0.081 10.030 [0.030 [0.028 [0.027 |0.026 [0.025 |0.021 on total weight of NPAH [Appearance [Bad Good [Good [Good [Good [Good [Good [Bad | [ND [Aspect qe [Good [Good [Good [Good [Good Bad | ss [particle range (nm) [ND [ND |9.7 [503 [102.1 362 |472 |469 [Turbidity (NTU) [127 [245 |169 [1309 |229 [218 [256 [Evaluations] (Appearance) The appearance of the compositions according to Examples 21-26 and Comparative Examples 10-11 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Frost The results are shown on the line marked "Aspect" in Table 6. (Particle size) The particle size or diameter of NPAH in the compositions according to Examples 21-26 and Comparative Examples 10-11 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at temperature ambient (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 6. ND (not available) in Table 6 means that no nanoparticles were found. (Turbidity) The turbidity of the compositions according to Examples 21-26 and Comparative Examples 10-11 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 6. (Summary) The experimental data shown in Table 6 show that if the weight ratio of the amount of the (c) non-polymeric acid(s) or salt(s) thereof, relative to the total weight of the particles, is less than or equal to 0.022, the particles cannot have a nanometric size (less than 1000 nm), and that if the amount of the (c) non-polymeric acid(s) or salt(s) is equal to zero, a gel forms. [Examples 27 to 32 and Comparative Examples 12 to 13] [Preparation] Each of the compositions according to Examples 27 to 32 and Comparative Examples 12 to 13 was prepared by mixing the ingredients listed in Table 7. The numerical values ​​of the quantities of ingredients in Table 7 are all based on a "% by weight" of active substances. The compositions according to Examples 27-32 and Comparative Examples 12-13 included particles containing hyaluronic acid. "NPAH" in Table 7 is an abbreviation for Hyaluronic Acid NanoParticle. (a) to (f) in Table 7 correspond to those of the claims. [Tables 7] 0.031 |0.031 [0.031 |0.031 [0.031 [0.031 [0.031 |0.031 - comp. |comp. |comp. |[comp.|comp. |[comp. |Ex. Ex. 27 28 29 30 31 32 |Ex.12 |Ex.13 pr (-|Hyaluronate [0.05 |0.05 |0.05 |0.05 |0.05 0.05 [0.05 Joos H 1) |of sodium (20-50 kDa) rare 0.1 Jos Jos Jos Jos Jos Jo: Jos 2) (a) |Polylysine | |0.031 [0.031 [0.031 [0.031 J0.031 [0.031 [0.031 [0.031 10.007 |0.007 [0.007 10.007 |0.007 0.007 |0.007 |0.007 03 [03 [03 [03 [0.3 03 03 0.3 [eo Acide 0,021 |0.021 [0,021 |0,021 [0,021 |0,021 |0,021 |0.021 phytique Q PCAde |- [0.010 |0,020 [0,038 [0.050 |o,080 [0,100 [0.200 calcium [eo Acide 0,007 [0.007 [0,007 [0,007 |0,007 [0,007 |0,007 [0,007 |0,007 oléique Caprylyl Jo.3 Jo3 Jo3 lo3 Jo3 lo3 Jo3 os Glycol Glycérine |t0 |to io 10 jo jo io jo Pentylène |s |s |s |s |s |s |s |5 |s glycol Propyène [5 [5 5 js |s 5 |5 |s glycol Oléate de po-|0,05 |o.05 |0,05 Joos [0.05 |o,05 0,05 “Joos lyglycéryle-2 Propylene |5 5 js |s |s js | |s glycol Oleate of po-J0.05 |0.05 |0.05 |0.05 |0.05 |0.05 |0.05 |0.05 0.05 lyglyceryl-2 Caprated [0.45 |0.45 |0.45 |0.45 |0.45 |0.45 |0.45 0.45 polyglyceryl 4 (D) |Water asp asp asp asp Jasp |asp las 100 |qsp 100 100 |100 j100 |100 |100 |100 NPAH Concentration [0.21 |0.22 |0.23 [0.25 |0.26 |0.29 |0.31 0.41 (% by weight) Weight Ratio of 0.239 [0.228 [0.218 [0.202 [0.193 [0.173 [0.162 |0.122 0.478 | 0.456 [ 0.437 | 0.405 | 0.386 | 0.346 [ 0.323 | 0.244 (b-1) of total NPAH weight ratio of (b-2) to total NPAH weight 0.046 | 0.087 [ 0.154 [ 0.193 [ 0.277 0.323 10.489 [0.033 [0.032 (0.031 [0.028 [0.027 [0.024 [0.023 [0.017 Weight ratio of (a) |0.148 [0.142 [0.135 [0.126 [0.120 [0.107 [0.101 |0.076 on total weight of NPAH Weight ratio of (c) [0.102 [0.096 |0.092 |0.085 [0.081 [0.073 [0.068 |0.052 on total weight of NPAH Weight ratio of (d) | 0.046 |0.087 [0.154 [0.193 [0.277 0.323 10.489 on total NPAH weight Ratio by weight of (e) [0.033 [0.032 |0.031 |0.028 [0.027 [0.024 |0.023 [0.017 on total NPAH weight [Appearance [Good [Good [Good |Good [Good |Good |Bad Bad |Good [Good |Good [Good |Good |Good |Good [Good Bad | Bad ss | 569.3 > 1000 | > 1000 [Particle size (nm) 33.5 |40.3 |39.2 |549 [75.6 [569.3 >1000 |>1000 Turbidity (NTU) 2.79 |3.89 |5.26 |187 [51.9 161 |167 |185 [Evaluations] (Appearance) The appearance of the compositions according to Examples 27-32 and Comparative Examples 12-13 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Precipitation has been observed The results are shown on the line marked "Aspect" in Table 7. (Particle size) The particle size or diameter of NPAH in the compositions according to Examples 27-32 and Comparative Examples 12-13 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 7. (Turbidity) The turbidity of the compositions according to Examples 27-32 and Comparative Examples 12-13 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 3. (Summary) The experimental data shown in Table 7 show that if the weight ratio of the amount of (d) metal salt to the total weight of the particles is 0.323 or more, the particles cannot have a nanometric size (less than 1000 nm), and precipitation occurs. [Reference Example 2 and Examples 33-39] [Preparation] Each of the compositions according to Reference Example 2 and Examples 33-39 was prepared by mixing the ingredients listed in Table 8. The numerical values ​​of the quantities of ingredients in Table 8 are all based on a "% by weight" of active substances, The compositions according to Reference Example 2 and Examples 33-39 included nanoparticles containing hyaluronic acid. "NPAH" in Table 8 is an abbreviation for Hyaluronic Acid Nanoparticle. (a) to (f) in Table 8 correspond to those of the claims. [Tables8] |Re.2 comp. |comp. |[comp. |Jcomp. |comp. |comp. comp. | 33 34 35 36 37 38 39 e (0.05 [0.05 [0.05 0.05 |0.05 |0.05 0.05 |0.05 NPA |(b-|Hyaluronate [0.05 |0.05 |0.05 0.05 |0.05 |0.05 |0.05 |0.05 H 1) |of sodium (20-50 kKDa) Gerets 1 61 jor Jon Jos Jor jor Jos 2) (a) |Polysine [0.03. |0.031 [0.031 |0.031 J0.031 [0.031 |0.03 | [0.001 |0.003 |0.005 |0.007 [0.010 [0.030 |0.050 | 03 103 03 03 [03 0.3 [0.3 |0.3 [5 |s js |5 |s |s |s |s PPT 1 NN [is Acid 0.021 [0.021 [0.021 [0.021 |0.021 |0.021 |0.021 0.021 | phytic | PCA of [0.038 [0.038 [0.038 [0.038 [0.038] [0.038 [0.038] calcium [© Acid || [o001 [0.003 |0.005 [0.007 |0.010 |0.030 |o.050 | oleic Caprylyl [03 Jo3 |Jo3 Jo3 Jos Jo3 |o3 Jo3 Glycol of “is Jo qe a pe Pentylene |5 |s |s |s |s |s |s |s glycol Propyene [5 |5 |5 |5 |s |s |s | glycol Oleate of po-[0.05 [0.05 Joos Joos Joos Joos lyglyceryl-2 Capratede |0.45 Jo45 |o45 lo45 Jo4s Jo45 |o45 Jo,45 polyglyceryl 4 (© [Water asp jasp asp asp asp asp asp asp asp 100 |ioo |i00 |100 |100 |i00 |100 |100 NPAH concentration |0.24 |0.24 |0.24 |0.25 |0.25 |0.25 0.27 0.29 by weight) (b-1) |0.208 [0.207 [0.206 [0.204 [0.202 [0.200 [0.185 [0.172 | Weight ratio of (b-2) [0.417 [0.415 0.411 [0.408 [0.405 [0.400 [0.370 [0.346 | on total weight of NPAH Weight ratio of (a) |0.129 0.129 |0.128 |0.127 [0.126 |0.124 |0.115 Jo.107 | on total weight of NPAH Weight ratio of (c) |0.088 |0.087 |0.087 |0.086 |0.085 |0.084 |0.078 0.072 | of total NPAH weight ratio of (d) |0.158 [0.158 |0.156 [0.155 [0.154 [0.152 [0.141 [0.131 | of total NPAH weight ratio of (e) |- 0.004 |0.012 [0.020 |0.028 |0.040 |0.111 0.172 | of total NPAH weight Appearance Good |Good |Good |Good |Good |Good |Good |Good Particle size (nm) |34.3 |57.6 |49.5 |345 [33.1 |59.8 |68.6 |52.5 [Turbidity (NTU) 18.1 |34.4 |198 122 [16.5 23.6 |314 |22.7 [Reviews] (Appearance) The appearance of the compositions according to Reference Example 2 and Examples 33 to 39 was assessed visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Turbid The results are shown on the line marked "Aspect" in Table 8. (Particle size) The particle size or diameter of NPAH in the compositions according to Reference Example 2 and Example 33-39 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 8. (Turbidity) The turbidity of the compositions according to Reference Example 2 and Examples 33-39 was measured by a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked “Turbidity (NTU)” in Table s. (Summary) The experimental data shown in Table 8 demonstrate that the nano- particles can be prepared, without depending on the quantity of the (e) fatty acid. However, in the absence of (e) fatty acid (Reference Example 2), the skin penetration enhancement effects derived from (e) fatty acid cannot be achieved. [Examples 40 to 44] [Preparation] Each of the compositions according to Examples 40 to 44 was prepared by mixing the ingredients listed in Table 9. The numerical values ​​of the quantities of ingredients in Table 9 are all based on a "% by weight" of active substances. The compositions according to Examples 40 to 44 included nanoparticles containing hyaluronic acid. "NPAH" in Table 9 is an abbreviation for Hyaluronic Acid Nanoparticle. (a) to (f) in Table 9 correspond to those in the claims. [Tables 9] (b-2 |Carrageenan ox ) Polylysine 0.031 (Ce) Phytic acid 0.021 (d) |Calcium PCA 0.038 Oleic acid 0.007 Caprylyl Glycol 0.3 Glycerin 0.007 Pentylene glycol 0.5 Propylene glycol = Polyglyceryl-2 oleate [0.05 Polyglyceryl-4 caprate |0.45 (D) |Water qs 100 NPAH concentration (% by weight) 0.25 Weight ratio of (b-1) to total weight of [0.202 NPAH Weight ratio of (b-2) to total weight of 0.405 NPAH total weight of |0.085 |0.085 |0.085 |0.085 |0.085 total weight of [0.154 [0.154 [0.154 [0.154 |0.154 | total weight of |0.028 |0.028 |0.028 |0.028 |0.028 Weight ratio of (a) to total weight of [0.126 [0.126 [0.126 [0.126 [0.126 NPAH] Weight ratio of (c) to total weight of |0.085 |0.085 |0.085 |0.085 |0.085 NPAH] Weight ratio of (d) to total weight of 0.154 [0.154 |0.154 |0.154 [0.154 NPAH] Weight ratio of (e) to total weight of [0.028 [0.028 [0.028 [0.028 0.028 NPAH] Appearance Good |Good Good |Good Good Particle size (nm) 45.5 Turbidity (NTU) 165 |232 |177 |124 218 Appearance Good |Good Good |Good Good Particle size (nm) 45.5 Lurbidity (NTU) 16.5 (232 [177 [124 [218 [Evaluations] (Appearance) The appearance of the compositions according to examples 40 to 44 was assessed visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Turbid The results are shown on the line marked "Aspect" in Table 9. (Particle size) The particle size or diameter of NPAH in the compositions according to Example 40-44 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 9. (Turbidity) The turbidity of the compositions according to Examples 40 to 44 was measured using a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line labeled "Turbidity (NTU)" in Table 9. (Summary) The experimental data shown in Table 9 demonstrate that a variety of hyaluronic acids can be used as the (β-1) first anionic polymer. [Examples 45 to 54] [Preparation] Each of the compositions in Examples 45 to 54 was prepared by mixing the ingredients listed in Table 10. The numerical values ​​of the quantities The ingredients in Table 10 are all based on a "% by weight" of active ingredients. The compositions in Examples 45 to 54 included nanoparticles containing hyaluronic acid. "NPAH" in Table 10 is an abbreviation for Hyaluronic Acid Nanoparticle. (a) to (f) in Table 10 correspond to those in the claims. [Tables 10] com |com |com |Jcom PP. |P- |P- |P- 49 |50 |S1 52 0.05 J0.05 J0.05 J0.05 |o.1 0.1 | jo.1 |o.1 [0.1 [0.1 Jo. | a” jo. | = = = 2 (a |Polylysine |003 |- |0.03 [0.03 |0.03 |0.08 |o. 0.03 [0.03 |0.03 10.03 1 1 1 1 [0.02 [0.02 |0.02 |0.02 1 li 1 qi | |- 001 | |E (+ 0.017 0.03 10.03 8 8 [= |- [0.03 |- [= | [= | J= (0.03 |- |- PS8 | [0.03 |- 8 0.00 |0.00 |-|- 7 7 | Jo! po - n (e |Stearic acid |e) |A|- - 0.00 | -7 0.00 7 0.3 0.3 (0.3 10 |i0 |10 5 5 45 [5 |s \|s [0.05 0.05 |0.05 0.45 10.45 [0.45 qsp |asP [asp 100 [100 |100 Continued Concentration in 0.25 |0.25 |0.25 NPAH (% by weight) Weight ratio of [0.20 |0.20 [0.20 [0.21 |0.20 [0.20 |0.20 [0.20 [0.20 |0.20 | (b-1) to total weight |2 1 6 1 2 2 2 2 2 2 of NPAH Weight ratio of |0.40 |0.40 |0.41 |0.42 [0.40 |0.40 |0.40 |0.40 [0.40 [0.40 | (b-2) to total weight |S 2 2 O 5 s 5 5 5 5 of NPAH Weight ratio of |0.12 |0.13 |0.12 |0.13 |0.12 |0.12 |0.12 [0.12 |0.12 0.12 | (a) on total weight of |6 2 7 oO 6 6 6 6 6 6 NPAH 0.08 |0.08 |0.07 [0.05 |0.08 |0.08 [0.08 J0.08 [0.08 008 | 5 4 0 0 5 5 5 5 5 5 Weight ratio of |0.08 |0.08 |0.07 |0.05 |0.08 [0.08 |0.08 [0.08 |0.08 0.08 | (c) on total weight of |5 4 0 oO 5 5 5 5 5 5 NPAH Weight ratio of |0.15 |0.15 |0.15 [0.16 |0.15 |0.15 [0.15 |0.15 |0.15 |0.15 (d) on total weight of |4 3 6 0 4 4 4 4 4 4 NPAH Weight ratio of |0.02 10.02 [0.02 |0.02 10.02 [0.02 [0.02 |0.02 [0.02 00 | (e) on total weight of |8 8 9 9 8 8 8 8 8 8 NPAH [Appearance [Good |Good |Good [Good [Good [Good [Good |Good |Good [Good [Good | Particle Size 74.2 |145, [86 41.3 |238, |465, 133.8 133.8 [33.3 326 | (nm) 3 7 6 Turbidity (NTU) 34 80 |42.6 127.4 101 |140 |17.6 |15.4 13.4 |69.4 [Reviews] (Appearance) The appearance of the compositions according to examples 45 to 54 was evaluated visually at room temperature (25 °C) in accordance with the following criteria. Good: Transparent Bad: Turbid The results are shown in the row titled "Aspect" in Table 10. (Particle size) The particle size or diameter of NPAH in the compositions according to Example 45-55 was measured by an ELSZ-2000 particle size analyzer by Otsuka Electronics Co., Ltd. at room temperature (25 °C). The results are shown on the line titled "Particle size (nm)" in Table 10. (Turbidity) The turbidity of the compositions according to Examples 45 to 55 was measured using a 2100Q turbidimeter (Hach Company) at room temperature (25 °C). The results are shown on the line marked "Turbidity (NTU)" in Table 10. (Summary) The experimental data shown in Table 10 show that a variety of (a) cationic polymers, (b-2) second anionic polymers, (c) non-polymeric acid or salt(s) thereof, (d) metal salts and (e) fatty acids can be used.

Claims

Claims

1. Composition, preferably cosmetic composition, and more preferably- cosmetic composition for the skin, comprising nanoparticles comprising: (a) at least one cationic polymer; (b-1) at least one first anionic polymer chosen from acids hyaluronics and their derivatives; (b-2) at least one second anionic polymer which is different from (b-1) first anionic polymer; (c) at least one non-polymeric acid or salt thereof; (d) at least one optional metal salt; and (e) at least one fatty acid, And (f) water, in which the amount of nanoparticles is 0.01% to 0.58% by weight, preferably, from 0.05% to 0.57% by weight and more preferably, from 0.1% to 0.56% by weight, relative to the total weight of the composition, the weight ratio of the quantity of the (a) cationic polymer(s) relative to the total weight of nanoparticles is 0.06 to 0.25, the weight ratio of the amount of the (b-2) second anionic polymer relative to the total weight of nanoparticles is 0.26 or more, the weight ratio of the quantity of the (c) non-po- lymeric(s) or salt(s) thereof relative to the total weight of the nano- particles is 0.03 or more, the weight ratio of the quantity of the (d) metal salt(s) fa- optional(s) relative to the total weight of nanoparticles is 0.30 or less, and the weight ratio of the quantity of the fatty acid(s) to to the total weight of nanoparticles is 0.001 or more.

2. A composition according to claim 1, wherein the (a) ca- polymer tionic comprises at least one positively chargeable moiety and / or positively charged selected from the group consisting of a group primary, secondary or tertiary amino, a qua- ammonium group ternary, a guanidine group, a biguanide group, a group imidazole, an imino group and a pyridyl group.

3. A composition according to claim 1 or 2, wherein the (a) polymer cationic is selected from the group consisting of cyclopolymers of alkyldiallylamine and dialkyldiallylammonium cyclopolymers such that (co)polydiallyldialkyl ammonium chloride, (co)polyamines such as chitosans and (co)polylysines, (co)polyamino acids cationic such as collagen, and an arginine / lysine polypeptide, the cationic cellulose polymers, and their salts.

4. Composition according to any one of claims 1 to 3, in which the (b-1) first anionic polymer has a molecular weight of 2,000 kDa or less, preferably 1,000 kDa or less, and more preferably- potentially 100 kDa or less.

5. Composition according to any one of claims 1 to 4, in which the weight ratio of the quantity of the first (b-1) anionic polymer(s) relative to the total weight of nanoparticles in the composition is from 0.01 to 0.8, preferably from 0.02 to 0.7, and more preferably from 0.03 to 0.

6.

6. Composition according to any one of claims 1 to 5, in which the (b-2) second anionic polymer is selected from the group consisting of carrageenan, pectin and a mixture thereof.

7. A composition according to any one of claims 1 to 6, in which the weight ratio of the quantity of the (b-2) second(s) anionic polymer(s) relative to the total weight of nanoparticles in the composition is from 0.26 to 0.9, preferably from 0.28 to 0.8, and more preferably from 0.30 to 0.

7.

8. A composition according to any one of claims | to 7, in which the (c) non-polymeric acid or salt(s) thereof is (are) selected from the group consisting of phytic acid, citric acid, lactic acid and a mixture of these.

9. A composition according to any one of claims 1 to 8, in which the (d) optional metal salt is selected from metal salts polyvalent, preferably divalent metal salts, more preferably- entially salts such as calcium chloride and pyrrolidone car- calcium carboxylate, and magnesium salts such as magnesium chloride magnesium.

10. A composition according to any one of claims 1 to 9, in in which the (e) fatty acid is chosen from saturated fatty acids and unsaturated, linear or branched C,-C», preferably Ce-Czp, more pre- ferentially Cz-Cir.