Method of controlling a fluidic valve of a fluidic system

Abstract

The invention relates to a method for controlling a fluidic valve (V) of a fluidic system, the fluidic valve (V) comprising an inlet fluidic channel (11) and an outlet fluidic channel (12), and a membrane (13) arranged between the inlet fluidic channel and the outlet fluidic channel, deformable between two distinct positions, the fluidic system comprising a device for actuating the membrane (13) between its two distinct positions, the actuating device comprising a hydraulic circuit and a pneumatic circuit, said pneumatic circuit being connected to the hydraulic circuit. Figure to be published with the abstract: Figure 2

Description

Title of the invention: Method for controlling a fluidic valve of a fluidic system Technical field of the invention

[0001] The present invention relates to a method for controlling a fluidic valve of a fluidic system. State of the art

[0002] A fluidic valve commonly used in a microfluidic card comprises a membrane that can be deformed between two positions to allow or block the passage of fluid between an inlet channel and an outlet channel. This type of valve is often actuated using pneumatic means connected to a control channel opening at the membrane. Exercising positive or negative pressure through this control channel allows the membrane to be deformed between its two positions.

[0003] The deformable membranes used at the microfluidic valves of a microfluidic card are permeable to gases. Maintaining them in a state by pneumatic effect thus causes the appearance of air bubbles, which can thus propagate in the microfluidic network because of this permeability. The risk of injecting bubbles into the microfluidic network is all the greater when the pressure applied under the membrane to maintain it in position is high and the duration of the application of the pressure is long.

[0004] To solve this problem, it would be possible to use hydraulic control means. However, this type of control creates other problems.

[0005] The aim of the invention is to propose a method for controlling a fluidic valve, using a deformable membrane, which can avoid problems linked to the gas permeability of the membrane and thus the introduction of air bubbles into the fluidic circuit, when the membrane is kept in a given state for a long time. Presentation of the invention

[0006] This object is achieved by a method for controlling a fluidic valve of a fluidic system, the fluidic valve comprising an inlet fluidic channel and an outlet fluidic channel, and a membrane arranged between the inlet fluidic channel and the outlet fluidic channel, deformable between two distinct positions, an open position to allow passage of a fluid from the inlet fluidic channel to the outlet fluidic channel and a closed position to block the passage of the fluid from the inlet fluidic channel to the outlet fluidic channel, the fluidic system comprising: - A device for actuating the membrane between its two distinct positions, the actuating device comprising a hydraulic circuit and a pneumatic circuit, said pneumatic circuit being connected to the hydraulic circuit, - The hydraulic circuit comprising an actuation channel and a priming channel, said priming channel comprising an inlet and an outlet, said actuation channel being connected on one side to the priming channel between its inlet and its outlet and opening on the other side opposite the membrane, - The pneumatic circuit comprising at least one control channel comprising an input intended to be connected to pneumatic means, followed by a connecting channel which communicates with the priming channel, - Said method comprising steps of: - Filling the priming channel of the hydraulic circuit through its inlet using an actuating liquid, - Closing the inlet and outlet of the priming channel, - Applying pressure in the pneumatic circuit inside the control channel to push the actuating liquid present in the priming channel towards the inside of the actuating channel.

[0007] According to a particular feature, the method comprises a step of applying a positive pressure in the pneumatic circuit to push the actuating liquid against the membrane and move it.

[0008] According to another feature, the pneumatic circuit comprises a buffer reservoir positioned on the connecting channel, the latter forming a cavity enlarged compared to the volume of the control channel.

[0009] According to another feature, the connecting channel is connected as close as possible to the inlet and / or the outlet of the priming channel.

[0010] The invention also relates to a fluidic system capable of implementing the control method as defined above, the fluidic system comprising a fluidic valve, said fluidic valve comprising an inlet fluidic channel and an outlet fluidic channel, and a membrane arranged between the inlet fluidic channel and the outlet fluidic channel, deformable between two distinct positions, an open position to allow passage of a fluid from the inlet fluidic channel to the outlet fluidic channel and a closed position to block the passage of the fluid from the inlet fluidic channel to the outlet fluidic channel, the fluidic system comprising: - A device for actuating the membrane between its two distinct positions, the actuating device comprising a hydraulic circuit and a pneumatic circuit, said pneumatic circuit being connected to the hydraulic circuit, - The hydraulic circuit comprising an actuation channel and a priming channel, said priming channel comprising an inlet and an outlet, said actuation channel being connected on one side to the priming channel between its inlet and its outlet and opening on the other side opposite the membrane, - The pneumatic circuit comprising at least one control channel comprising an input intended to be connected to pneumatic means, followed by a connecting channel which communicates with the priming channel.

[0011] According to a particular feature, the connecting channel is connected as close as possible to the inlet and / or the outlet of the priming channel.

[0012] According to another feature, the pneumatic circuit comprises a buffer reservoir positioned on the connecting channel, forming a cavity enlarged relative to the volume of the connecting channel.

[0013] According to another feature, the connecting channel has a fluid resistance greater than that of the priming channel.

[0014] According to another feature, the connecting channel has a hydrophobic internal surface.

[0015] According to another feature, the actuation channel has a volume which is at least 50% smaller than that of the priming channel. Brief description of the figures

[0016] Other characteristics and advantages will appear in the detailed description which follows, given with reference to the appended drawings in which: - Figures IA and IB illustrate the operating principle of a fluidic valve; - [Fig.2] shows an example of embodiment of the fluidic system of the invention; - Figures 3A to 3C illustrate the operating principle of the fluidic system of the invention; - [Fig.4] represents a first advantageous variant embodiment of the invention; - Figures 5A and 5B show a second advantageous variant embodiment of the invention and illustrate its operating principle; - [Fig.6] illustrates a manufacturing principle of the fluidic system of the invention;

[0017] Detailed description of at least one embodiment

[0018] For the remainder of the description, an orthonormal reference frame X, Y, Z is defined, the Z axis being oriented in the vertical direction. The terms "upper", "lower", "above" and "below" or equivalent are to be understood as following the Z axis. Microfluidic card and fluidic valve

[0019] [Fig.lA]

[0020] [Fig.lB]

[0021] The invention applies to the control of a fluidic valve V conventionally used in a microfluidic card.

[0022] A microfluidic card is used in particular in the medical field to analyze a fluid, such as a liquid sample (blood for example) by connecting to an analysis machine. The microfluidic card can thus integrate an entire microfluidic network composed of microfluidic elements such as valves, chambers and microfluidic channels.

[0023] A microfluidic card can be made in a single layer or by assembling several layers together. Its layers are for example assembled together by thermal sealing. Each layer can be machined so as to create at least part of the microfluidic network of the card, the assembly of the layers by stacking making it possible to form the entire microfluidic network of the microfluidic card.

[0024] The microfluidic card has two opposite faces, each extending in the two dimensions X, Y and has a small thickness (a few mm) along Z with respect to the other two dimensions.

[0025] The microfluidic card is advantageously made of a transparent material of the COP (Cyclo-Olefin Polymer), COC (Cyclo-Olefin Copolymer), PMMA (Polymethyl Acrylic Methacrylate), PDMS (Polydimethyl Siloxane), Silicon type, etc.

[0026] The microfluidic network of the microfluidic card may comprise one or more fluidic valves.

[0027] The fluidic valve V conventionally comprises a cavity 10 into which an inlet fluidic channel 11 and an outlet fluidic channel 12 open. The fluidic valve also comprises a membrane 13 deformable between at least two positions inside the cavity, an open position in which it allows the passage of fluid from the inlet fluidic channel 11 to the outlet fluidic channel 12, through the cavity 10 ([Fig.lA]), and a closed position, in which it deploys inside the cavity 10 to block the passage of fluid between the inlet fluidic channel 11 and the outlet fluidic channel 12 ([Fig.lB]). In its closed position, the membrane 13 is for example kept pressed against a wall of the cavity, closing the passage present between the two channels via the cavity.

[0028] The membrane 13 is for example interposed between two layers of the microfluidic card and is sealed between these two layers.

[0029] To actuate the membrane 13 between its two positions, the microfluidic card comprises an actuating channel 20 opening opposite the membrane 13. This actuating channel 20 is part of an actuating device of the fluidic system.

[0030] In the context of the invention, the actuating device consists of a hydraulic circuit and a pneumatic circuit. The membrane is actuated between its two positions using an actuating liquid and the actuating liquid is moved using the pneumatic circuit, by applying a positive or negative pressure in the pneumatic circuit. Actuating device

[0031] [Fig.2]

[0032] According to the invention, the actuating device therefore consists of a hydraulic circuit and a pneumatic circuit.

[0033] The hydraulic circuit and the pneumatic circuit are advantageously integrated into the layers of the microfluidic card.

[0034] The hydraulic circuit is intended to be filled with an actuating liquid L, coming from a liquid reservoir 2, and the pneumatic circuit is intended to be filled with a gas (for example air), activating a pneumatic system 3 (compressor for example).

[0035] The hydraulic circuit comprises the actuation channel 20 described above, as well as a priming channel 21.

[0036] The actuating channel 20 has two distinct ends, a first end opening opposite the membrane 13 of the fluidic valve V and a second end connected to the priming channel 21.

[0037] The priming channel 21 also comprises two distinct ends, a first end forming an inlet 210 for the actuating liquid and a second end forming an outlet 211. The actuating channel 20 is therefore connected to the priming channel 21, between its inlet 210 and its outlet 211. The terms "inlet" and "outlet" are to be considered in a non-limiting manner and their role can be reversed.

[0038] In a non-limiting manner, the inlet and outlet of the priming channel 21 are accessible from the outside of the microfluidic card, for example on its upper face, so as to be able to inject the actuating liquid L therein.

[0039] The pneumatic circuit comprises for its part a control channel 30 integrated into the microfluidic card, which also comprises two distinct ends, a first end which communicates directly with the priming channel 21, via a connecting channel 40, and a second end which connects to a pneumatic system 3. By "communicates directly", we mean that the air injected into the control channel 30 comes into physical contact with the actuating liquid L present in the hydraulic circuit and that there is no intermediate wall or specific membrane separating the two circuits.

[0040] The connecting channel 40 forms the junction between the control channel 30 and the priming channel 21 (possibly via a buffer tank 31 - see below).

[0041] When the actuating liquid L is introduced into the priming channel 21, via the inlet of this channel, it fills the priming channel 21, without penetrating into the connecting channel 40.

[0042] It is also noted that the air trapped in the actuation channel 20 makes it possible to prevent the actuation liquid L from penetrating into the actuation channel 20.

[0043] When filling the priming channel 21, one of the objectives of the invention is in particular that the channel 40 does not fill with actuating liquid L, then the two circuits are in direct communication. To achieve this objective, different solutions can be envisaged: • The cross-section of the connecting channel 40 is chosen to be smaller than that of the priming channel 21. This is why the fluid resistance of the channel 40 is higher than that of the channel 21. During the filling (step E1 - see below) of the priming channel 21, the actuating liquid L therefore preferably flows towards the outlet 211 of the priming channel, and not towards the connecting channel 40. • Channel 40 has an internal surface of a hydrophobic nature, which reinforces the effect described above.

[0044] Similarly, when actuating the fluidic valve V between its two positions, these principles remain valid to limit the risk of penetration of the actuating liquid L into the connecting channel 40. This is particularly the case when applying negative pressure to open the valve. Functioning

[0045] [Fig.3A]

[0046] [Fig.3B]

[0047] [Fig.3C]

[0048] The operating principle of the system is described below.

[0049] El - [Fig.3A]: The actuating liquid L is introduced into the priming channel 21 via its inlet 210. When the actuating liquid L is introduced into the priming channel 21, it occupies the entire priming channel 21, without penetrating into the connecting channel 40. The excess injected liquid can be recovered via the outlet 211 of the priming channel 21.

[0050] E2: The inlet 210 and the outlet 211 of the priming channel 21 are closed, for example using adhesives 50 stuck to the upper face of the microfluidic card. The actuating liquid L thus occupies the priming channel 21, without having penetrated the actuating channel 20. Residual air bubbles may be present at the ends of the priming channel 21.

[0051] E3: A positive pressure is applied in the control channel 30 of the pneumatic circuit, using the pneumatic system 3.

[0052] By applying this pressure, the injected air pushes the actuating liquid L present in the priming channel 21 towards the inside of the actuating channel 20, until the liquid occupies the entire actuating channel 20. An air pocket (meniscus) can be created at the inlet and / or outlet of the priming channel 21.

[0053] The actuating device is thus ready for use to actuate the fluidic valve. To actuate the fluidic valve V, it is then sufficient to control the pneumatic system 3 so that it applies a positive pressure in the control channel 30, in order to push the actuating liquid L present in the actuating channel 20. Pressurized, the actuating liquid L pushes the membrane 13 and deforms it.

[0054] It should be noted that there is an advantage in the total volume of the actuation channel 20 being particularly small compared to that of the priming channel 21. Thus, the time required to implement this step E3 is short and the loss of liquid present in the priming channel 21 to fill the actuation channel 20 is low. In addition, the presence of a small volume at the actuation channel 20 presents a significant advantage during actuation under negative pressure to open the fluidic valve V. The displacement of the liquid thus remains small compared to the total volume of the actuation liquid L present in the priming channel 21. If this displaced volume were larger, there would be more risk of the liquid being sucked into the connecting channel 40.

[0055] By way of example, the volume of the actuation channel 20 is at least 50% smaller than that of the priming channel 21, for example 75% smaller than that of the priming channel 21. Implementation variants

[0056] [Fig.4]

[0057] [Fig.5A]

[0058] [Fig.5B]

[0059] According to a particular feature, the connecting channel 40 is arranged so as to open as close as possible to the outlet of the priming channel 21, this in order to prevent the actuating liquid L from escaping by gravity and / or capillarity.

[0060] Preferably, the connection channel 40 is directly connected to the output 211 of the priming channel 21 ([Fig.4]).

[0061] Advantageously, the connecting channel 40 is directly connected to the outlet 211 of the priming channel 21 but also to the inlet 210 of the priming channel 21 ([Fig.5A]). In this way, when the positive pressure is applied inside the control channel 30, the air pushes the actuating liquid L in parallel through the inlet 210 of the priming channel 21 and through the outlet 211 of the priming channel 21, to push it towards the inside of the actuating channel 20 ([Fig.5B]).

[0062] In these last two configurations, the connecting channel 40 is produced by a cavity hollowed out on the upper face of the microfluidic card to connect the control channel 30 to the inlet and outlet of the priming channel 21. During step E2 above, said cavity is then completely closed from above, so as to ensure sealed communication between the control channel 30 and the priming channel 21.

[0063] Advantageously, the pneumatic circuit comprises a buffer tank 31 positioned on the connecting channel 40 and used to store any excess actuating liquid which would flow back from the hydraulic circuit, and thus avoid damaging the pneumatic system connected to the control channel 30.

[0064] Advantageously, the internal wall of the connecting channel 40 can be coated, at least in part, with a hydrophobic compound 32, in particular in its portion opening into the priming channel 21, this in order to limit any reflux of actuating liquid L towards the pneumatic system 3. It should be noted that a material such as COC is basically rather hydrophobic.

[0065] It should be noted that it is also advantageous to leave a residual air bubble at the connection of the connecting channel 40 to the priming channel 21, in order to limit any backflow of liquid. The connecting channel 40 will advantageously have a reduced cross-section, to avoid backflow of liquid by capillarity.

[0066] According to an alternative embodiment, the inlet 210 and / or the outlet 211 of the priming channel 21 can be arranged to open onto the lower face of the microfluidic card 1. Manufacturing

[0067] [Fig.6]

[0068] The microfluidic card is for example produced by assembling several layers sealed together.

[0069] In a non-limiting manner, we have for example: - A first lower layer Cl which comprises a through hole for the input of the control channel 30; - A second layer C2 stacked on the first layer, which comprises impressions to form the initiation channel 21, the actuation channel 20, and a portion of the control channel 30. - A third layer C3 stacked on the second layer, consisting of the deformable membrane 13. The membrane 13 can be cut on certain targeted areas which correspond to fluid passages. - A fourth layer C4 stacked on the third layer, comprising the microfluidic network, in particular the fluidic valve V, and the portions of the priming channel 21 forming its inlet and its outlet, as well as the control channel 30, the buffer reservoir 31 and the cavity forming the connecting channel 40 connecting the control channel 30 to the inlet and the outlet of the priming channel 21. - A final upper layer C5, formed by one or more adhesives 50 closing said cavity from above.

[0070] During manufacturing, it will be noted that it is relevant that: - The actuation channel 20 has a low volume, in order to have little volume of liquid to move when actuation of the fluidic valve V; - The connecting channel 40 also has a small volume, to increase its fluid resistance and limit any reflux of liquid towards the control channel 30;

[0071] The invention thus presents numerous advantages, among which: - A solution that overcomes the problems of gas permeability of membranes, by offering actuation by a hydraulic circuit; - A solution that allows you to manage the filling of the hydraulic circuit, limiting the appearance of air bubbles; - A solution equipped with means to prevent the backflow of liquid into the pneumatic circuit, even though the hydraulic circuit and the pneumatic circuit are in direct communication;

Claims

Claims

1. Method for controlling a fluidic valve (V) of a fluidic system, the fluidic valve (V) comprising an inlet fluidic channel (11) and an outlet fluidic channel (12), and a membrane (13) arranged between the inlet fluidic channel and the outlet fluidic channel, deformable between two distinct positions, an open position to allow passage of a fluid from the inlet fluidic channel to the outlet fluidic channel and a closed position to block the passage of the fluid from the inlet fluidic channel to the outlet fluidic channel, the fluidic system comprising: A device for actuating the membrane (13) between its two separate positions, the actuating device comprising a hydraulic circuit and a pneumatic circuit, said pneumatic circuit being connected to the hydraulic circuit, The hydraulic circuit comprising an actuating channel (20) and a priming channel (21), said priming channel (21) comprising an inlet (210) and an outlet (211), said actuating channel (20) being connected on one side to the priming channel between its inlet and its outlet and opening on the other side opposite the membrane (13), The pneumatic circuit comprising at least one control channel (30) comprising an inlet intended to be connected to pneumatic means, followed by a connecting channel (40) which communicates with the priming channel (21), Said method being characterized in that it comprises steps of: Filling the priming channel (21) of the hydraulic circuit through its inlet with an actuating liquid (L), Closing the inlet and outlet of the priming channel (21), Applying pressure in the pneumatic circuit inside the control channel (30) to push the actuating liquid (L) present in the priming channel (21) towards the inside of the actuating channel (20).

2. Method according to claim 1, characterized in that it comprises a step of applying a positive pressure in the pneumatic circuit to push the actuating liquid (L) against the membrane (13) and move it.

3. Method according to claim 1 or 2, characterized in that the pneumatic circuit comprises a buffer reservoir (31) positioned on the connecting channel (40) and which forms a cavity enlarged compared to the volume of the control channel.

4. Method according to one of claims 1 to 3, characterized in that the connecting channel (40) is connected as close as possible to the inlet and / or the outlet of the priming channel (21).

5. Fluidic system comprising a fluidic valve (V), said fluidic valve comprising an inlet fluidic channel (11) and an outlet fluidic channel (12), and a membrane (13) arranged between the inlet fluidic channel and the outlet fluidic channel, deformable between two distinct positions, an open position to allow passage of a fluid from the inlet fluidic channel to the outlet fluidic channel and a closed position to block the passage of the fluid from the inlet fluidic channel to the outlet fluidic channel, characterized in that the fluidic system comprises: - A device for actuating the membrane (13) between its two distinct positions, the actuating device comprising a hydraulic circuit and a pneumatic circuit, said pneumatic circuit being connected to the hydraulic circuit, - The hydraulic circuit comprising an actuating channel (20) and a priming channel (21),said priming channel (21) comprising an inlet (210) and an outlet (211), said actuating channel (20) being connected on one side to the priming channel between its inlet and its outlet and opening on the other side opposite the membrane (13), - The pneumatic circuit comprising at least one control channel (30) comprising an inlet intended to be connected to pneumatic means, followed by a connecting channel which communicates with the priming channel (21) and in that:,

6.

7.

8.

9.

10. - Said hydraulic circuit and said pneumatic circuit are capable of being controlled according to the control method as defined in one of claims 1 to 4. System according to claim 5, characterized in that the connecting channel (40) is connected as close as possible to the inlet and / or the outlet of the priming channel (21). System according to claim 5 or 6, characterized in that the pneumatic circuit comprises a buffer tank (31) positioned on the connecting channel (40) and which forms a cavity enlarged compared to the volume of the control channel. System according to one of claims 5 to 7, characterized in that the connecting channel (40) has a fluid resistance greater than that of the priming channel (21). System according to one of claims 5 to 8, characterized in that the connecting channel (40) has a hydrophobic internal surface. System according to one of claims 5 to 9, characterized in that the actuation channel (20) has a volume which is at least 50% smaller than that of the priming channel (21).

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